SMED30018035

Overview
NameSMED30018035
Smed IDSMED30018035
Length (bp)1467
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018035 (SMED30018035) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018035 (SMED30018035) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018035 (SMED30018035) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018035 (SMED30018035) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30018035

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30018035h1SMcG0019554 SmedASXL_000463SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30018035h1SMcG0019554 SmedASXL_000214SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
zeta neoblastSMED30018035h1SMcG0019554 dd_Smed_v4_50305_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018035 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001173:11838..13289 +7260
Homology
BLAST of SMED30018035 vs. Planmine SMEST
Match: SMESG000015075.1 (SMESG000015075.1)

HSP 1 Score: 777.319 bits (2006), Expect = 0.000e+0
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 1
Query:   58 MLEISKSCFICYSDILETYGFSDCMAYHTFCLQCITHLYLNDWEYYKCPFDRMINKQIVTNINGKLKPFSNYSILFSNFEIEKSIGKCLFSILLLIADKLDAYIHLLTEMINXXXXXXXXXXXLLVRYTSDNSHSIPSVLNMQSQLKSDRLEIRKLLHNNCYLFDKLCRIYFSSNLSSFETILNELTELQMKRIDLNHHFQMLRFEAINRNYFDKDEYANLFGNDGKFQINNCVLCLKYHMLTNSVKLVDCQHILXXXXXXXXXXXXFICPIDLIKSQINNSFQSVEQXXXXXXXXXXXXTVLNIYKIDNQFISSCRDNKIKFYNQFFRFPQGIIREDLCFIPDDKTIHXXXXXXXXXFHRLYWRSLAGNYDLKKLYKRVATLIFGCHHQHEYLMIFKQYLVDSECLEQTVVIKTAINNLIKIFSYNFLETV 1353
            MLEISKSCFICYSDILETYGFSDCMAYHTFCLQCITHLYLNDWEYYKCPFDRMINKQIVTNINGKLKPFSNYSILFSNFEIEKSIGKCLFSILLLIADKLDAYIHLLTEMINIIITTTRPRTILLVRYTSDNSHSIPSVLNMQSQLKSDRLEIRKLLHNNCYLFDKLCRIYFSSNLSSFETILNELTELQMKRIDLNHHFQMLRFEAINRNYFDKDEYANLFGNDGKFQINNCVLCLKYHMLTNSVKLVDCQHILCESCAESVESESFICPIDLIKSQINNSFQSVEQSSSENVLNLLSETVLNIYKIDNQFISSCRDNKIKFYNQFFRFPQGIIREDLCFIPDDKTIHENLLEELENFHRLYWRSLAGNYDLKKLYKRVATLIFGCHHQHEYLMIFKQYLVDSECLEQTVVIKTAINNLIKIFSYNFLETV
Sbjct:    1 MLEISKSCFICYSDILETYGFSDCMAYHTFCLQCITHLYLNDWEYYKCPFDRMINKQIVTNINGKLKPFSNYSILFSNFEIEKSIGKCLFSILLLIADKLDAYIHLLTEMINIIITTTRPRTILLVRYTSDNSHSIPSVLNMQSQLKSDRLEIRKLLHNNCYLFDKLCRIYFSSNLSSFETILNELTELQMKRIDLNHHFQMLRFEAINRNYFDKDEYANLFGNDGKFQINNCVLCLKYHMLTNSVKLVDCQHILCESCAESVESESFICPIDLIKSQINNSFQSVEQSSSENVLNLLSETVLNIYKIDNQFISSCRDNKIKFYNQFFRFPQGIIREDLCFIPDDKTIHENLLEELENFHRLYWRSLAGNYDLKKLYKRVATLIFGCHHQHEYLMIFKQYLVDSECLEQTVVIKTAINNLIKIFSYNFLETV 432          
The following BLAST results are available for this feature:
BLAST of SMED30018035 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018035 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000015075.10.000e+0100.00SMESG000015075.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018035 ID=SMED30018035|Name=SMED30018035|organism=Schmidtea mediterranea sexual|type=transcript|length=1467bp
CAGCGAGTAATAGTAATATTCAATAATATTTGACCTCAAATAATTTTACA
TTTCAAAATGTTAGAAATATCCAAAAGTTGCTTTATTTGTTATAGTGATA
TTTTGGAAACTTATGGATTCTCAGACTGTATGGCTTATCACACATTTTGT
TTACAATGTATCACGCATTTATATTTAAATGACTGGGAGTATTATAAATG
CCCCTTTGATAGAATGATCAACAAACAAATTGTTACAAATATTAATGGAA
AATTGAAACCATTTAGCAACTATTCTATTTTATTCTCAAATTTTGAAATT
GAAAAGTCAATAGGCAAATGTTTATTTAGTATATTACTATTAATCGCTGA
CAAACTCGATGCATATATTCATTTACTTACAGAAATGATCAATATTATCA
TTACAACAACTAGACCAAGGACTATTTTGCTTGTTCGATATACCTCAGAC
AATTCCCACTCAATTCCTTCAGTTCTAAATATGCAAAGCCAATTGAAATC
GGATCGTCTGGAAATTAGAAAACTGTTACACAACAATTGCTATTTGTTTG
ACAAGCTTTGTAGAATCTATTTCTCGTCTAACCTCTCATCGTTTGAAACG
ATTTTGAATGAACTCACAGAATTGCAAATGAAACGAATTGATCTAAATCA
TCATTTTCAAATGTTGAGATTCGAAGCAATAAATCGAAACTACTTCGACA
AAGATGAATACGCAAATCTATTCGGAAACGATGGGAAATTCCAAATTAAT
AATTGCGTACTCTGCTTAAAGTACCATATGTTGACAAATAGCGTCAAATT
GGTTGATTGTCAACATATTTTGTGTGAATCCTGTGCCGAGTCGGTGGAAA
GTGAAAGTTTCATTTGTCCGATCGATTTAATTAAATCGCAAATCAATAAT
AGTTTTCAATCGGTTGAACAATCTTCCTCAGAAAATGTGTTGAATTTATT
ATCAGAAACAGTCTTGAATATCTATAAAATCGATAATCAATTTATTAGCA
GCTGCCGAGACAATAAAATCAAATTTTACAACCAATTCTTTCGATTTCCC
CAAGGAATTATCAGAGAGGATTTATGTTTCATTCCCGACGACAAAACAAT
CCATGAAAATCTATTAGAGGAATTGGAAAATTTCCATCGATTATATTGGA
GATCATTAGCGGGAAATTACGATTTGAAGAAACTTTACAAGAGAGTTGCA
ACGTTGATTTTCGGTTGTCATCATCAGCATGAATATTTGATGATTTTCAA
GCAATATCTCGTCGATTCGGAATGTCTGGAGCAAACTGTCGTTATCAAAA
CGGCAATTAACAATCTAATCAAGATATTTTCATACAATTTTCTTGAGACT
GTTTGAAATAGATTTGTTTATATAACTTTTGTGTTTTCATTAACGCAGCT
AATTCTGAAAATCGGTGGAATATGATTGGTTAGCTCATAAAATTGTAATT
GCCCAATCAAAATTTGG
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protein sequence of SMED30018035-orf-1

>SMED30018035-orf-1 ID=SMED30018035-orf-1|Name=SMED30018035-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=433bp
MLEISKSCFICYSDILETYGFSDCMAYHTFCLQCITHLYLNDWEYYKCPF
DRMINKQIVTNINGKLKPFSNYSILFSNFEIEKSIGKCLFSILLLIADKL
DAYIHLLTEMINIIITTTRPRTILLVRYTSDNSHSIPSVLNMQSQLKSDR
LEIRKLLHNNCYLFDKLCRIYFSSNLSSFETILNELTELQMKRIDLNHHF
QMLRFEAINRNYFDKDEYANLFGNDGKFQINNCVLCLKYHMLTNSVKLVD
CQHILCESCAESVESESFICPIDLIKSQINNSFQSVEQSSSENVLNLLSE
TVLNIYKIDNQFISSCRDNKIKFYNQFFRFPQGIIREDLCFIPDDKTIHE
NLLEELENFHRLYWRSLAGNYDLKKLYKRVATLIFGCHHQHEYLMIFKQY
LVDSECLEQTVVIKTAINNLIKIFSYNFLETV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
IPR017907Znf_RING_CS
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 233..273
e-value: 0.018
score: 24.2
coord: 8..51
e-value: 0.52
score: 13.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 8..52
score: 8.772
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 233..273
score: 9.893
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 251..260
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 7..58
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 232..273