Kunitz-type protease inhibitor

Overview


Name	Kunitz-type protease inhibitor
Smed ID	SMED30017992
Length (bp)	431

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Kunitz-type protease inhibitor (SMED30017992) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Kunitz-type protease inhibitor (SMED30017992) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30017992

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30017992	SMESG000037999.1 SMESG000037995.1	Contig6004	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30017992	SMESG000037999.1 SMESG000037995.1	Contig6004	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
nervous system	SMED30017992	SMESG000037999.1 SMESG000037995.1	dd_Smed_v4_1072_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30017992	SMESG000037999.1 SMESG000037995.1	dd_Smed_v4_1072_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30017992	SMESG000037999.1 SMESG000037995.1	dd_Smed_v4_1072_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017992	SMESG000038002.1 SMESG000037999.1 SMESG000037997.1 SMESG000037995.1	Contig44493	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017992	SMESG000038002.1 SMESG000037999.1 SMESG000037997.1 SMESG000037995.1	Contig44493	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
epidermis	SMED30017992	SMESG000038002.1 SMESG000037999.1 SMESG000037997.1 SMESG000037995.1	Contig44493	newmark_ests	PMID:27612384 Roberts-Galbraith et al., 2016
epidermis	SMED30017992	SMESG000038002.1 SMESG000037999.1 SMESG000037997.1 SMESG000037995.1	Contig44493	uc_Smed_v2	PMID:27612384 Roberts-Galbraith et al., 2016
nervous system	SMED30017992	SMESG000037999.1 SMESG000037995.1	dd_Smed_v4_1072_0_1	dd_Smed_v4	PMID:30962434 Cote et al., 2019

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 68.9366 bits (167), Expect = 6.076e-14
Identity = 28/54 (51.85%), Postives = 39/54 (72.22%), Query Frame = 3

Query:  207 LKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            L  C+ P   G CKAY  R+ +NS+TG+C++F+YGGC  NGNNF++ + CEE C
Sbjct:  290 LAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 343

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 68.5514 bits (166), Expect = 8.337e-14
Identity = 28/54 (51.85%), Postives = 39/54 (72.22%), Query Frame = 3

Query:  207 LKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            L  C+ P   G CKAY  R+ +NS+TG+C++F+YGGC  NGNNF++ + CEE C
Sbjct:  383 LAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 436

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: SPINT3 (serine peptidase inhibitor, Kunitz type 3 [Source:HGNC Symbol;Acc:HGNC:11248])

HSP 1 Score: 59.6918 bits (143), Expect = 3.646e-12
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  P + G C+ Y  R++FN ETG+CE F YGGCG N NNF  +++CE+ C
Sbjct:   36 CAFPMEKGPCQTYMTRWFFNFETGECELFAYGGCGGNSNNFLRKEKCEKFC 86

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 63.5438 bits (153), Expect = 5.198e-12
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C++   +G C+A   R+YF+   GKC  FIYGGCG N NNF++ED C  +C
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVC 360

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 63.5438 bits (153), Expect = 5.208e-12
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C++   +G C+A   R+YF+   GKC  FIYGGCG N NNF++ED C  +C
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVC 360

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3d.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 62.3882 bits (150), Expect = 4.734e-12
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C  P  +G+C  Y  R++FNS+TG+CE F YG CG N NNF   + CE  C+
Sbjct: 2168 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 2219

HSP 2 Score: 61.2326 bits (147), Expect = 1.316e-11
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            KEC      G C   F+R+++N ++ KCE F Y GCG NGNN+++ + CE+ C
Sbjct:  421 KECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 3 Score: 59.3066 bits (142), Expect = 7.181e-11
Identity = 22/54 (40.74%), Postives = 33/54 (61.11%), Query Frame = 3

Query:  207 LKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            +  C  P+ SG C+  F R++F+ E   C+ F Y GC  NGNNF +++EC  +C
Sbjct:  725 VNRCLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 778

HSP 4 Score: 58.9214 bits (141), Expect = 8.792e-11
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+ PK+ G C  Y  RFYFN++  +C+ F +GGC  N NNF+  ++CE  C
Sbjct:  539 CSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 5 Score: 57.3806 bits (137), Expect = 2.688e-10
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            ++C +P + G CK +  R+YFN + G C  F YGGC  N N+F T+ ECE  C
Sbjct: 1072 EKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1124

HSP 6 Score: 53.9138 bits (128), Expect = 4.137e-9
Identity = 22/52 (42.31%), Postives = 26/52 (50.00%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C    + G C   F RF F+     C +F YGGCG N NNF T  EC   C+
Sbjct:  857 CEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 908

HSP 7 Score: 51.2174 bits (121), Expect = 4.619e-8
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+  K SG  K Y  ++YFN +  +CE F++ G G N N F+T  ECE +C
Sbjct: 2232 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 2282

HSP 8 Score: 49.2914 bits (116), Expect = 1.987e-7
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C +P   G C+    R+Y++  T  C  F Y GC  N NNF + ++C+ LC+
Sbjct: 2733 CIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCV 2784

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 62.3882 bits (150), Expect = 4.734e-12
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C  P  +G+C  Y  R++FNS+TG+CE F YG CG N NNF   + CE  C+
Sbjct: 2177 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 2228

HSP 2 Score: 61.2326 bits (147), Expect = 1.291e-11
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            KEC      G C   F+R+++N ++ KCE F Y GCG NGNN+++ + CE+ C
Sbjct:  421 KECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 3 Score: 59.3066 bits (142), Expect = 7.181e-11
Identity = 22/54 (40.74%), Postives = 33/54 (61.11%), Query Frame = 3

Query:  207 LKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            +  C  P+ SG C+  F R++F+ E   C+ F Y GC  NGNNF +++EC  +C
Sbjct:  734 VNRCLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 787

HSP 4 Score: 58.9214 bits (141), Expect = 8.708e-11
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+ PK+ G C  Y  RFYFN++  +C+ F +GGC  N NNF+  ++CE  C
Sbjct:  539 CSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 5 Score: 57.3806 bits (137), Expect = 2.663e-10
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            ++C +P + G CK +  R+YFN + G C  F YGGC  N N+F T+ ECE  C
Sbjct: 1081 EKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1133

HSP 6 Score: 53.9138 bits (128), Expect = 4.138e-9
Identity = 22/52 (42.31%), Postives = 26/52 (50.00%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C    + G C   F RF F+     C +F YGGCG N NNF T  EC   C+
Sbjct:  866 CEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 917

HSP 7 Score: 51.2174 bits (121), Expect = 4.531e-8
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+  K SG  K Y  ++YFN +  +CE F++ G G N N F+T  ECE +C
Sbjct: 2241 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 2291

HSP 8 Score: 49.2914 bits (116), Expect = 1.987e-7
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C +P   G C+    R+Y++  T  C  F Y GC  N NNF + ++C+ LC+
Sbjct: 2742 CIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCV 2793

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 62.3882 bits (150), Expect = 5.108e-12
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C  P  +G+C  Y  R++FNS+TG+CE F YG CG N NNF   + CE  C+
Sbjct: 2048 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 2099

HSP 2 Score: 61.2326 bits (147), Expect = 1.434e-11
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            KEC      G C   F+R+++N ++ KCE F Y GCG NGNN+++ + CE+ C
Sbjct:  292 KECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 344

HSP 3 Score: 58.9214 bits (141), Expect = 7.899e-11
Identity = 22/54 (40.74%), Postives = 33/54 (61.11%), Query Frame = 3

Query:  207 LKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            +  C  P+ SG C+  F R++F+ E   C+ F Y GC  NGNNF +++EC  +C
Sbjct:  605 VNRCLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 658

HSP 4 Score: 58.9214 bits (141), Expect = 9.305e-11
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+ PK+ G C  Y  RFYFN++  +C+ F +GGC  N NNF+  ++CE  C
Sbjct:  410 CSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 460

HSP 5 Score: 57.3806 bits (137), Expect = 2.873e-10
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            ++C +P + G CK +  R+YFN + G C  F YGGC  N N+F T+ ECE  C
Sbjct:  952 EKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1004

HSP 6 Score: 53.9138 bits (128), Expect = 4.421e-9
Identity = 22/52 (42.31%), Postives = 26/52 (50.00%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C    + G C   F RF F+     C +F YGGCG N NNF T  EC   C+
Sbjct:  737 CEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 788

HSP 7 Score: 50.8322 bits (120), Expect = 4.888e-8
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+  K SG  K Y  ++YFN +  +CE F++ G G N N F+T  ECE +C
Sbjct: 2112 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 2162

HSP 8 Score: 49.2914 bits (116), Expect = 2.103e-7
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C +P   G C+    R+Y++  T  C  F Y GC  N NNF + ++C+ LC+
Sbjct: 2613 CIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCV 2664

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 61.6178 bits (148), Expect = 9.161e-12
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C  P  +G+C  Y  R++FNS+TG+CE F YG CG N NNF   + CE  C+
Sbjct: 1651 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 1702

HSP 2 Score: 60.4622 bits (145), Expect = 2.450e-11
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            KEC      G C   F+R+++N ++ KCE F Y GCG NGNN+++ + CE+ C
Sbjct:  421 KECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 3 Score: 58.5362 bits (140), Expect = 1.349e-10
Identity = 22/54 (40.74%), Postives = 33/54 (61.11%), Query Frame = 3

Query:  207 LKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            +  C  P+ SG C+  F R++F+ E   C+ F Y GC  NGNNF +++EC  +C
Sbjct:  734 VNRCLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 787

HSP 4 Score: 58.151 bits (139), Expect = 1.605e-10
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+ PK+ G C  Y  RFYFN++  +C+ F +GGC  N NNF+  ++CE  C
Sbjct:  539 CSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 5 Score: 56.6102 bits (135), Expect = 4.858e-10
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            ++C +P + G CK +  R+YFN + G C  F YGGC  N N+F T+ ECE  C
Sbjct: 1081 EKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1133

HSP 6 Score: 53.5286 bits (127), Expect = 6.918e-9
Identity = 22/52 (42.31%), Postives = 26/52 (50.00%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C    + G C   F RF F+     C +F YGGCG N NNF T  EC   C+
Sbjct:  866 CEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 917

HSP 7 Score: 50.447 bits (119), Expect = 7.571e-8
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+  K SG  K Y  ++YFN +  +CE F++ G G N N F+T  ECE +C
Sbjct: 1715 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 1765

HSP 8 Score: 48.521 bits (114), Expect = 3.224e-7
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C +P   G C+    R+Y++  T  C  F Y GC  N NNF + ++C+ LC+
Sbjct: 2216 CIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCV 2267

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3h.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 61.6178 bits (148), Expect = 9.240e-12
Identity = 25/52 (48.08%), Postives = 33/52 (63.46%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C  P  +G+C  Y  R++FNS+TG+CE F YG CG N NNF   + CE  C+
Sbjct: 1575 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKCM 1626

HSP 2 Score: 60.4622 bits (145), Expect = 2.378e-11
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            KEC      G C   F+R+++N ++ KCE F Y GCG NGNN+++ + CE+ C
Sbjct:  200 KECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 252

HSP 3 Score: 58.5362 bits (140), Expect = 1.322e-10
Identity = 22/54 (40.74%), Postives = 33/54 (61.11%), Query Frame = 3

Query:  207 LKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            +  C  P+ SG C+  F R++F+ E   C+ F Y GC  NGNNF +++EC  +C
Sbjct:  513 VNRCLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 566

HSP 4 Score: 58.151 bits (139), Expect = 1.619e-10
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+ PK+ G C  Y  RFYFN++  +C+ F +GGC  N NNF+  ++CE  C
Sbjct:  318 CSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 368

HSP 5 Score: 56.6102 bits (135), Expect = 4.853e-10
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            ++C +P + G CK +  R+YFN + G C  F YGGC  N N+F T+ ECE  C
Sbjct:  860 EKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 912

HSP 6 Score: 53.5286 bits (127), Expect = 6.979e-9
Identity = 22/52 (42.31%), Postives = 26/52 (50.00%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C    + G C   F RF F+     C +F YGGCG N NNF T  EC   C+
Sbjct:  645 CEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCV 696

HSP 7 Score: 50.447 bits (119), Expect = 7.491e-8
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+  K SG  K Y  ++YFN +  +CE F++ G G N N F+T  ECE +C
Sbjct: 1639 CSFDKDSGSGKGYNVKWYFNMKNLRCEQFVFEGLGGNTNQFETLSECERIC 1689

HSP 8 Score: 48.521 bits (114), Expect = 3.284e-7
Identity = 19/52 (36.54%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C +P   G C+    R+Y++  T  C  F Y GC  N NNF + ++C+ LC+
Sbjct: 2140 CIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLCV 2191

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0077545)

HSP 1 Score: 63.929 bits (154), Expect = 1.180e-13
Identity = 24/54 (44.44%), Postives = 41/54 (75.93%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            ++C++PK++G C A F R+ +N +T  CE F+YGGC  N NNF+++++CE+ C+
Sbjct:   53 EDCHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQACL 106

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0346581)

HSP 1 Score: 63.929 bits (154), Expect = 1.180e-13
Identity = 24/54 (44.44%), Postives = 41/54 (75.93%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            ++C++PK++G C A F R+ +N +T  CE F+YGGC  N NNF+++++CE+ C+
Sbjct:   53 EDCHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQACL 106

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: CG15418 (gene:FBgn0031554 transcript:FBtr0077556)

HSP 1 Score: 56.9954 bits (136), Expect = 2.449e-11
Identity = 23/57 (40.35%), Postives = 36/57 (63.16%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            ++  C +PK  G+C+ +  R+ +N +TG CE+FIY GC    NNF T +EC   C++
Sbjct:   37 QIIACRQPKAPGLCRGHQLRYAYNKKTGNCESFIYTGCASTENNFLTFEECRRDCMQ 93

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: CG15418 (gene:FBgn0031554 transcript:FBtr0446100)

HSP 1 Score: 56.9954 bits (136), Expect = 2.449e-11
Identity = 23/57 (40.35%), Postives = 36/57 (63.16%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            ++  C +PK  G+C+ +  R+ +N +TG CE+FIY GC    NNF T +EC   C++
Sbjct:   37 QIIACRQPKAPGLCRGHQLRYAYNKKTGNCESFIYTGCASTENNFLTFEECRRDCMQ 93

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: CG34276 (gene:FBgn0085305 transcript:FBtr0112471)

HSP 1 Score: 53.5286 bits (127), Expect = 4.211e-10
Identity = 20/55 (36.36%), Postives = 34/55 (61.82%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            + C +P   G  KAY + +++NS + +C+ FI+ G   NGNNF T+  C ++C+ 
Sbjct:   24 RRCLQPLDVGKGKAYLRNWFYNSTSQRCQRFIFYGGASNGNNFNTQARCHKICLA 78

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Match: appb (amyloid beta (A4) precursor protein b [Source:ZFIN;Acc:ZDB-GENE-020220-1])

HSP 1 Score: 63.5438 bits (153), Expect = 3.237e-12
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  P +SG C A   R+YF +E G+C +F +GGCG N NNF++E+ C  +C
Sbjct:  294 CWAPARSGPCHAKLPRWYFVAEKGRCASFTFGGCGGNRNNFESEEYCMAVC 344

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Match: appb (amyloid beta (A4) precursor protein b [Source:ZFIN;Acc:ZDB-GENE-020220-1])

HSP 1 Score: 63.5438 bits (153), Expect = 3.307e-12
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  P +SG C A   R+YF +E G+C +F +GGCG N NNF++E+ C  +C
Sbjct:  306 CWAPARSGPCHAKLPRWYFVAEKGRCASFTFGGCGGNRNNFESEEYCMAVC 356

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Match: col7a1 (collagen, type VII, alpha 1 [Source:ZFIN;Acc:ZDB-GENE-030131-2427])

HSP 1 Score: 63.5438 bits (153), Expect = 3.379e-12
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            C  P + G C  Y  R+YFNS+   C  FIY GCG N N +  ++ECE+LC++
Sbjct: 3040 CILPMEEGDCSRYTLRWYFNSQVDACRPFIYSGCGGNANRYLQKEECEKLCLQ 3092

[Close]

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            C  P + G C  Y  R+YFNS+   C  FIY GCG N N +  ++ECE+LC++
Sbjct: 3040 CILPMEEGDCSRYTLRWYFNSQVDACRPFIYSGCGGNANRYLQKEECEKLCLQ 3092

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Match: appb (amyloid beta (A4) precursor protein b [Source:ZFIN;Acc:ZDB-GENE-020220-1])

HSP 1 Score: 63.5438 bits (153), Expect = 3.532e-12
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  P +SG C A   R+YF +E G+C +F +GGCG N NNF++E+ C  +C
Sbjct:  295 CWAPARSGPCHAKLPRWYFVAEKGRCASFTFGGCGGNRNNFESEEYCMAVC 345

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:NCBI gene;Acc:100486381])

HSP 1 Score: 65.855 bits (159), Expect = 7.278e-13
Identity = 27/51 (52.94%), Postives = 38/51 (74.51%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            CN P   GMCKAY  R+ +N +  +C++FIYGGCG N NNF+T++ CE++C
Sbjct:  370 CNFPALQGMCKAYKPRWAYNKQLKQCQSFIYGGCGGNDNNFETKETCEDMC 420

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:Xenbase;Acc:XB-GENE-976485])

HSP 1 Score: 65.4698 bits (158), Expect = 1.152e-12
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            CN  K+ G C+ +  ++Y++ ET  C  F YGGCG N N F T+ ECE++CI
Sbjct: 2829 CNLQKEEGTCRDFVLKWYYDPETKSCARFWYGGCGGNENRFSTQKECEKICI 2880

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:Xenbase;Acc:XB-GENE-976485])

HSP 1 Score: 65.4698 bits (158), Expect = 1.154e-12
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            CN  K+ G C+ +  ++Y++ ET  C  F YGGCG N N F T+ ECE++CI
Sbjct: 3009 CNLQKEEGTCRDFVLKWYYDPETKSCARFWYGGCGGNENRFSTQKECEKICI 3060

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:Xenbase;Acc:XB-GENE-976485])

HSP 1 Score: 65.4698 bits (158), Expect = 1.154e-12
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            CN  K+ G C+ +  ++Y++ ET  C  F YGGCG N N F T+ ECE++CI
Sbjct: 3026 CNLQKEEGTCRDFVLKWYYDPETKSCARFWYGGCGGNENRFSTQKECEKICI 3077

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 61.2326 bits (147), Expect = 3.180e-11
Identity = 24/51 (47.06%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C++   +G C+A   R+YF+    KC  FIYGGCG N NNF++ED C  +C
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVC 347

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: Eppin (epididymal peptidase inhibitor [Source:MGI Symbol;Acc:MGI:1922776])

HSP 1 Score: 64.3142 bits (155), Expect = 1.129e-13
Identity = 27/51 (52.94%), Postives = 35/51 (68.63%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+ PK SG C AYF+R++FN E   C+ FIYGGC  N NNFQ++  C+  C
Sbjct:   77 CSLPKDSGYCMAYFRRWWFNKENSTCQVFIYGGCQGNNNNFQSQSICQNAC 127

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: Wfikkn2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:MGI Symbol;Acc:MGI:2669209])

HSP 1 Score: 66.6254 bits (161), Expect = 3.348e-13
Identity = 27/51 (52.94%), Postives = 37/51 (72.55%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+ P   G CKAY  R+ +NS+TG C++F+YGGC  NGNNF++ + CEE C
Sbjct:  381 CSLPALQGPCKAYVPRWAYNSQTGLCQSFVYGGCEGNGNNFESREACEESC 431

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: Spint3 (serine peptidase inhibitor, Kunitz type, 3 [Source:MGI Symbol;Acc:MGI:3651470])

HSP 1 Score: 60.8474 bits (146), Expect = 8.723e-13
Identity = 27/51 (52.94%), Postives = 35/51 (68.63%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  PK  G C+A F R+Y+NS+TGKCE F YGGC  N NNF +  +C+ +C
Sbjct:   35 CILPKDIGGCRAVFVRWYYNSKTGKCEWFRYGGCKGNENNFPSRSQCQAVC 85

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: Spint5 (serine protease inhibitor, Kunitz type 5 [Source:MGI Symbol;Acc:MGI:3651687])

HSP 1 Score: 60.4622 bits (145), Expect = 2.174e-12
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            CN+P + G C   F R+YFN E+  CE+FI+ GCG N NNF+T+  CE  CI 
Sbjct:   49 CNQPVKKGFCSFTFYRYYFNPESALCESFIFTGCGGNRNNFKTKYLCEVRCIH 101

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: Spint5 (serine protease inhibitor, Kunitz type 5 [Source:MGI Symbol;Acc:MGI:3651687])

HSP 1 Score: 60.4622 bits (145), Expect = 2.221e-12
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            CN+P + G C   F R+YFN E+  CE+FI+ GCG N NNF+T+  CE  CI 
Sbjct:   49 CNQPVKKGFCSFTFYRYYFNPESALCESFIFTGCGGNRNNFKTKYLCEVRCIH 101

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|B2G331|VKT1_HETCR (TauPI-stichotoxin-Hcr2b OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 6.093e-17
Identity = 33/51 (64.71%), Postives = 37/51 (72.55%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C EPK  G C AYF+RFYF+SETGKC  FIYGGC  NGNNF+T   C  +C
Sbjct:   26 CLEPKVVGPCTAYFRRFYFDSETGKCTVFIYGGCEGNGNNFETLRACRAIC 76

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DL86|VKT21_HETCR (Kunitz-type serine protease inhibitor HCRG21 OS=Heteractis crispa OX=175771 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 6.336e-17
Identity = 33/51 (64.71%), Postives = 38/51 (74.51%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+EPK  G C AYF+RFYF+SETGKC  FIYGGC  NGNNF+T   C  +C
Sbjct:    4 CSEPKVVGPCTAYFRRFYFDSETGKCTPFIYGGCEGNGNNFETLRACRAIC 54

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DMJ5|VKT_HETCR (PI-stichotoxin-Hcr2e OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.760e-16
Identity = 33/51 (64.71%), Postives = 36/51 (70.59%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C EPK  G C AYF RFYF+SETGKC  FIYGGC  NGNNF+T   C  +C
Sbjct:   26 CLEPKVVGPCTAYFPRFYFDSETGKCTPFIYGGCEGNGNNFETLHACRAIC 76

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|C0HJF4|VKT2_HETCR (TauPI-stichotoxin-Hcr2c OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.956e-16
Identity = 33/51 (64.71%), Postives = 37/51 (72.55%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C EPK  G C AYF+RFYF+SETGKC  FIYGGC  NGNNF+T   C  +C
Sbjct:    4 CLEPKVVGPCTAYFRRFYFDSETGKCTPFIYGGCEGNGNNFETLRACRAIC 54

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|C0HK74|VKT3_HETMG (Kunitz-type serine protease inhibitor HMGS2 OS=Heteractis magnifica OX=38281 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 2.593e-16
Identity = 33/51 (64.71%), Postives = 36/51 (70.59%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C EPK  G C AYF RFYF+SETGKC  FIYGGC  NGNNF+T   C  +C
Sbjct:    4 CLEPKVVGPCTAYFPRFYFDSETGKCTPFIYGGCEGNGNNFETLHACRAIC 54

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: A0A3G1GLI4 (Kunitz-type peptide HCRG21 (Fragment) OS=Heteractis crispa OX=175771 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.878e-14
Identity = 33/51 (64.71%), Postives = 38/51 (74.51%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+EPK  G C AYF+RFYF+SETGKC  FIYGGC  NGNNF+T   C  +C
Sbjct:    4 CSEPKVVGPCTAYFRRFYFDSETGKCTPFIYGGCEGNGNNFETLRACRAIC 54

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: B7P626 (Colostrum trypsin inhibitor, putative (Fragment) OS=Ixodes scapularis OX=6945 GN=8025167 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.044e-14
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C++PK SG C AY +RFY+NSET +CE FIYGGC  NGNNF T D+C   C
Sbjct:    9 CHQPKYSGPCYAYLRRFYYNSETERCEPFIYGGCMANGNNFYTMDQCNRHC 59

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: A0A3G1GLH1 (Kunitz-type peptide HMGS3c1 (Fragment) OS=Heteractis magnifica OX=38281 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 5.798e-14
Identity = 33/51 (64.71%), Postives = 37/51 (72.55%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C EPK  G C AYF+RFYF+SETGKC  FIYGGC  NGNNF+T   C  +C
Sbjct:    4 CLEPKVVGPCTAYFRRFYFDSETGKCTPFIYGGCEGNGNNFETLRACRAIC 54

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: A0A3G1GLH6 (Kunitz-type peptide HMGS3c4 (Fragment) OS=Heteractis magnifica OX=38281 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 7.123e-14
Identity = 33/51 (64.71%), Postives = 37/51 (72.55%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C EPK  G C AYF+RFYF+SETGKC  FIYGGC  NGNNF+T   C  +C
Sbjct:    4 CLEPKIVGPCTAYFRRFYFDSETGKCTPFIYGGCEGNGNNFETLRACRAIC 54

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: C3Z9Z8 (BPTI/Kunitz inhibitor domain-containing protein (Fragment) OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_163244 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 7.415e-14
Identity = 32/51 (62.75%), Postives = 39/51 (76.47%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C +PK+ G CKA F R +FNS+TG+CE FIYGGCG N NNF+T +ECE  C
Sbjct:    9 CQQPKKVGNCKAAFPRVFFNSQTGQCENFIYGGCGGNDNNFETAEECENTC 59

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: ENSAMXT00000015806.2 (tissue factor pathway inhibitor-like [Source:NCBI gene;Acc:103025270])

HSP 1 Score: 64.6994 bits (156), Expect = 6.217e-13
Identity = 28/48 (58.33%), Postives = 36/48 (75.00%), Query Frame = 3

Query:  228 KQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            K  G CKA   RFYF+ ++G+CE+F YGGC  N NNFQT D+CEE+C+
Sbjct:   40 KDEGPCKAVKDRFYFDIDSGRCESFEYGGCQGNANNFQTLDQCEEMCV 87

HSP 2 Score: 46.2098 bits (108), Expect = 2.192e-6
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+   + G C+    R++F+S   +C  F YGGC  N NNF+T  EC + C
Sbjct:   95 CHLEDEPGPCRGLVPRYFFDSRKQECRRFFYGGCFGNANNFKTLKECRDRC 145

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: appb (amyloid beta A4 protein-like [Source:NCBI gene;Acc:103046668])

HSP 1 Score: 64.3142 bits (155), Expect = 1.750e-12
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  P + G C+A   R+YF +ETG+C  F +GGCG N NNF++E+ C  +C
Sbjct:  311 CWAPPRRGPCRATLSRWYFVAETGRCAPFSFGGCGGNRNNFESEEYCMAVC 361

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: appb (amyloid beta A4 protein-like [Source:NCBI gene;Acc:103046668])

HSP 1 Score: 63.929 bits (154), Expect = 1.919e-12
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  P + G C+A   R+YF +ETG+C  F +GGCG N NNF++E+ C  +C
Sbjct:  311 CWAPPRRGPCRATLSRWYFVAETGRCAPFSFGGCGGNRNNFESEEYCMAVC 361

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: ENSAMXT00000053501.1 (pep primary_assembly:Astyanax_mexicanus-2.0:12:31379453:31384888:1 gene:ENSAMXG00000037311.1 transcript:ENSAMXT00000053501.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.225 bits (134), Expect = 2.578e-11
Identity = 23/47 (48.94%), Postives = 32/47 (68.09%), Query Frame = 3

Query:  228 KQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
             ++G C+A   R+YF  E G+C  FIYGGCG N NNF++E+ C  +C
Sbjct:   10 AETGPCRAMLPRWYFVKEEGRCAPFIYGGCGGNRNNFESEEYCLSVC 56

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: ENSAMXT00000002208.2 (pep primary_assembly:Astyanax_mexicanus-2.0:13:36140062:36218718:1 gene:ENSAMXG00000002013.2 transcript:ENSAMXT00000002208.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.077 bits (144), Expect = 5.694e-11
Identity = 22/52 (42.31%), Postives = 30/52 (57.69%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C  P   G C  Y   +YFNS+ G C  F+Y GCG N N +  ++ECE+ C+
Sbjct: 2228 CALPLDEGDCSRYTLHWYFNSQVGACRPFVYSGCGGNANRYTQKEECEKHCL 2279

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:ZFIN;Acc:ZDB-GENE-060503-38])

HSP 1 Score: 60.4622 bits (145), Expect = 3.181e-12
Identity = 26/51 (50.98%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  P + G C+A F R++FN  T +CE+FIYGGC  N NNF+T  EC   C
Sbjct:   27 CTLPAKPGDCRAAFPRYFFNRSTLRCESFIYGGCHSNENNFETMQECRAAC 77

HSP 2 Score: 51.9878 bits (123), Expect = 3.286e-9
Identity = 22/55 (40.00%), Postives = 32/55 (58.18%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            K C    ++G C+A+  R+++N   G C  F+YGGCG N N F+  + C   CIE
Sbjct:   85 KLCRPDAETGRCRAHITRYFYNMTLGMCMPFVYGGCGGNNNRFEDMESCVFSCIE 139

HSP 3 Score: 47.3654 bits (111), Expect = 1.719e-7
Identity = 21/55 (38.18%), Postives = 29/55 (52.73%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            +EC     +G C+  F+RFY+N +   C  F Y GCG N NNF +   C   C+ 
Sbjct:  145 RECLSRHDTGNCQGNFRRFYYNIQKRSCLPFSYSGCGGNNNNFISAKTCLSTCLS 199

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005252.1 (pep scaffold:Pmarinus_7.0:GL477257:283672:285029:-1 gene:ENSPMAG00000004785.1 transcript:ENSPMAT00000005252.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 3.185e-10
Identity = 23/51 (45.10%), Postives = 30/51 (58.82%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C  P  SG C+    R +FN+  G CE+F Y GCG NGN+F +   C+ LC
Sbjct:   65 CKLPADSGPCEESTYRIHFNASNGLCESFPYSGCGGNGNSFPSTQACQRLC 115

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008261.1 (pep scaffold:Pmarinus_7.0:GL478901:735:1448:1 gene:ENSPMAG00000007479.1 transcript:ENSPMAT00000008261.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 1.118e-9
Identity = 22/53 (41.51%), Postives = 31/53 (58.49%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            + C+ P   G CKA+  R+ +N    +C TF+YGGC  NGN+F +   CE  C
Sbjct:   19 RVCSLPPVQGPCKAWETRWSYNPLGKRCATFLYGGCEGNGNSFASRSACERAC 71

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 53.9138 bits (128), Expect = 1.127e-9
Identity = 23/51 (45.10%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C+ P Q G CK +  R+ +N    +C++FIYGGC  N NNFQ+++ CE+ C
Sbjct:  374 CSLPVQQGPCKQWALRWAYNPLIKRCQSFIYGGCEGNENNFQSQELCEDNC 424

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 1.398e-9
Identity = 23/53 (43.40%), Postives = 31/53 (58.49%), Query Frame = 3

Query:  234 SGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE*KYFWK 392
             G C A   RF++N +T  CE+F YGGCG N NNF +E  C  +C +  Y  +
Sbjct:  172 PGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVCGQVGYVQR 224

HSP 2 Score: 50.447 bits (119), Expect = 1.129e-8
Identity = 22/55 (40.00%), Postives = 32/55 (58.18%), Query Frame = 3

Query:  210 KECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            K C    + G+C+A  +R++FN  +G CE F YGGC  N NNF+    C + C+ 
Sbjct:   72 KACRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCVH 126

HSP 3 Score: 50.0618 bits (118), Expect = 1.876e-8
Identity = 22/45 (48.89%), Postives = 28/45 (62.22%), Query Frame = 3

Query:  234 SGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
             G C+AY  R ++N  T +CE F YGGC  NGNNF++  EC   C
Sbjct:    9 PGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO29819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2J6])

HSP 1 Score: 68.5514 bits (166), Expect = 1.604e-16
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            C  PK+ G C+A   R +FNS++GKCE FIYGGCG N NNF T +EC++ C+ 
Sbjct:   14 CTLPKKVGDCRAAMPRVFFNSQSGKCEDFIYGGCGGNANNFHTMEECKKACMS 66

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO38284 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDB9])

HSP 1 Score: 63.1586 bits (152), Expect = 2.371e-14
Identity = 26/52 (50.00%), Postives = 37/52 (71.15%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C  PK +G C A  +RFY+N++ GKC++F++GGC  NGNNF+T   CE  C+
Sbjct:   13 CKLPKFAGPCMASIERFYYNAKIGKCQSFVFGGCLPNGNNFKTRKACEAKCM 64

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 62.003 bits (149), Expect = 4.167e-14
Identity = 26/52 (50.00%), Postives = 40/52 (76.92%), Query Frame = 3

Query:  213 ECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            EC +PK +G C+AYF+R+++N  + KC+ F+YGGC  N NNF+++ ECE+ C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO26263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 62.003 bits (149), Expect = 4.167e-14
Identity = 26/52 (50.00%), Postives = 40/52 (76.92%), Query Frame = 3

Query:  213 ECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            EC +PK +G C+AYF+R+++N  + KC+ F+YGGC  N NNF+++ ECE+ C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO31760 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX09])

HSP 1 Score: 58.151 bits (139), Expect = 1.677e-12
Identity = 24/53 (45.28%), Postives = 34/53 (64.15%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            C+ PK  G+C+A  +R+Y+N+ET KC  F +GGC  N NNF T+  C   C+ 
Sbjct:    1 CDLPKNPGVCRASIQRWYYNNETRKCIKFTFGGCHGNANNFPTKSSCRNACMR 53

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: APP (amyloid-beta A4 protein [Source:NCBI gene;Acc:101162104])

HSP 1 Score: 62.003 bits (149), Expect = 1.101e-11
Identity = 23/51 (45.10%), Postives = 34/51 (66.67%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C    ++G C+A   R++F+ E G+C  FIYGGCG N NNF++E+ C  +C
Sbjct:  330 CWADAETGPCRALLPRWFFDREEGRCVQFIYGGCGGNRNNFESEEYCMSVC 380

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: appb (amyloid-beta A4 protein [Source:NCBI gene;Acc:101172288])

HSP 1 Score: 61.6178 bits (148), Expect = 1.170e-11
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            C    +SG C    +R+YF  E+G+C  F++GGCG N NNF+TE+ C  +C
Sbjct:  286 CWARAESGPCHGMLERWYFEPESGRCAPFLFGGCGGNRNNFETEEYCLAVC 336

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: ENSORLT00000012975.2 (pep primary_assembly:ASM223467v1:12:15740273:15760140:1 gene:ENSORLG00000010354.2 transcript:ENSORLT00000012975.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.6918 bits (143), Expect = 1.396e-11
Identity = 26/52 (50.00%), Postives = 35/52 (67.31%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C +   SG+C  YF+R+++N   G+CE FIYGGC  N NNF TE EC + C+
Sbjct:  125 CAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKECLQRCL 176

HSP 2 Score: 56.9954 bits (136), Expect = 1.462e-10
Identity = 25/52 (48.08%), Postives = 34/52 (65.38%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            C +   SG+C  YF+R+++N   G+CE FIYGGC  N NNF TE EC +  +
Sbjct:   73 CAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKECLQRWV 124

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: ENSORLT00000029029.1 (pep primary_assembly:ASM223467v1:5:3412877:3416364:1 gene:ENSORLG00000024614.1 transcript:ENSORLT00000029029.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.6102 bits (135), Expect = 1.944e-11
Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 3

Query:  222 EPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            E K+   C+ Y  R+YF+S+ G C  FIY GCG N N F  ++ECEELC+
Sbjct:    2 ELKRWQSCQRYTLRWYFSSQAGACRPFIYSGCGGNSNRFLHQEECEELCL 51

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: ENSORLT00000042019.1 (pep primary_assembly:ASM223467v1:12:15740119:15780496:1 gene:ENSORLG00000010354.2 transcript:ENSORLT00000042019.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.077 bits (144), Expect = 2.214e-11
Identity = 29/56 (51.79%), Postives = 38/56 (67.86%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCI 371
            K++ C +   SG+C  YF+R+++N   G+CE FIYGGC  N NNF TE EC  LCI
Sbjct:   21 KVRVCAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKEC--LCI 74

HSP 2 Score: 59.6918 bits (143), Expect = 3.352e-11
Identity = 27/51 (52.94%), Postives = 35/51 (68.63%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDEC 356
            KL + + P  SG+C  YF+R+++N   G+CE FIYGGC  N NNF TE EC
Sbjct:   77 KLPQPHIPPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKEC 127

HSP 3 Score: 48.1358 bits (113), Expect = 4.807e-7
Identity = 24/59 (40.68%), Postives = 32/59 (54.24%), Query Frame = 3

Query:  216 CNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE*KYFWK 392
            C      G C+ Y +R+++N +  +CE FIYG C  N NNF+TE EC   C    Y W 
Sbjct:  133 CTLDPDPGPCECYSERYFYNFKKRRCEIFIYGCCNGNQNNFETEQECLRRCRFHYYEWS 191

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000037999.1 (SMESG000037999.1)

HSP 1 Score: 116.701 bits (291), Expect = 5.934e-35
Identity = 55/57 (96.49%), Postives = 57/57 (100.00%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            +LKECNEPKQSGMC+AYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE
Sbjct:   23 RLKECNEPKQSGMCEAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 79

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000037999.1 (SMESG000037999.1)

HSP 1 Score: 116.316 bits (290), Expect = 6.534e-35
Identity = 55/57 (96.49%), Postives = 57/57 (100.00%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            +LKECNEPKQSGMC+AYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE
Sbjct:   21 RLKECNEPKQSGMCEAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 77

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000037995.1 (SMESG000037995.1)

HSP 1 Score: 116.316 bits (290), Expect = 7.303e-35
Identity = 55/57 (96.49%), Postives = 56/57 (98.25%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            +LKECNEPKQSGMC AYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE
Sbjct:   23 RLKECNEPKQSGMCMAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 79

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000037994.1 (SMESG000037994.1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.232e-22
Identity = 37/57 (64.91%), Postives = 45/57 (78.95%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELCIE 374
            +  ECNEPK++GMC AYF RFYFN ++G CE+FIYGGC  NGNNF T +ECE  C+E
Sbjct:   21 RAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARCLE 77

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000038052.1 (SMESG000038052.1)

HSP 1 Score: 69.707 bits (169), Expect = 1.479e-16
Identity = 31/55 (56.36%), Postives = 39/55 (70.91%), Query Frame = 3

Query:  204 KLKECNEPKQSGMCKAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 368
            K  ECN P ++G+C+AYF R+ F+ + GKC TF+YGGC  N NNF T  ECEE C
Sbjct:   21 KSPECNLPLETGLCEAYFVRYGFDVKLGKCRTFVYGGCHVNDNNFHTRAECEERC 75

The following BLAST results are available for this feature:

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
BLAST of Kunitz-type protease inhibitor vs. TrEMBL
BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
BLAST of Kunitz-type protease inhibitor vs. Ensembl Yeast
BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
WFIKKN2	6.076e-14	51.85	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
WFIKKN2	8.337e-14	51.85	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
SPINT3	3.646e-12	50.98	serine peptidase inhibitor, Kunitz type 3 [Source:...	[more]
APLP2	5.198e-12	49.02	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
APLP2	5.208e-12	49.02	amyloid beta precursor like protein 2 [Source:HGNC...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mlt-11	4.734e-12	48.08	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	4.734e-12	48.08	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	5.108e-12	48.08	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]
mlt-11	9.161e-12	48.08	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	9.240e-12	48.08	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CG3604	1.180e-13	44.44	gene:FBgn0031562 transcript:FBtr0077545	[more]
CG3604	1.180e-13	44.44	gene:FBgn0031562 transcript:FBtr0346581	[more]
CG15418	2.449e-11	40.35	gene:FBgn0031554 transcript:FBtr0077556	[more]
CG15418	2.449e-11	40.35	gene:FBgn0031554 transcript:FBtr0446100	[more]
CG34276	4.211e-10	36.36	gene:FBgn0085305 transcript:FBtr0112471	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
appb	3.237e-12	47.06	amyloid beta (A4) precursor protein b [Source:ZFIN...	[more]
appb	3.307e-12	47.06	amyloid beta (A4) precursor protein b [Source:ZFIN...	[more]
col7a1	3.379e-12	45.28	collagen, type VII, alpha 1 [Source:ZFIN;Acc:ZDB-G...	[more]
col7a1	3.379e-12	45.28	collagen, type VII, alpha 1 [Source:ZFIN;Acc:ZDB-G...	[more]
appb	3.532e-12	47.06	amyloid beta (A4) precursor protein b [Source:ZFIN...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
WFIKKN2	7.278e-13	52.94	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
itch	1.152e-12	46.15	itchy E3 ubiquitin protein ligase [Source:Xenbase;...	[more]
itch	1.154e-12	46.15	itchy E3 ubiquitin protein ligase [Source:Xenbase;...	[more]
itch	1.154e-12	46.15	itchy E3 ubiquitin protein ligase [Source:Xenbase;...	[more]
APLP2	3.180e-11	47.06	amyloid beta (A4) precursor-like protein 2 [Source...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Eppin	1.129e-13	52.94	epididymal peptidase inhibitor [Source:MGI Symbol;...	[more]
Wfikkn2	3.348e-13	52.94	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
Spint3	8.723e-13	52.94	serine peptidase inhibitor, Kunitz type, 3 [Source...	[more]
Spint5	2.174e-12	50.94	serine protease inhibitor, Kunitz type 5 [Source:M...	[more]
Spint5	2.221e-12	50.94	serine protease inhibitor, Kunitz type 5 [Source:M...	[more]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|B2G331\|VKT1_HETCR	6.093e-17	64.71	TauPI-stichotoxin-Hcr2b OS=Heteractis crispa OX=17...	[more]
sp\|P0DL86\|VKT21_HETCR	6.336e-17	64.71	Kunitz-type serine protease inhibitor HCRG21 OS=He...	[more]
sp\|P0DMJ5\|VKT_HETCR	1.760e-16	64.71	PI-stichotoxin-Hcr2e OS=Heteractis crispa OX=17577...	[more]
sp\|C0HJF4\|VKT2_HETCR	1.956e-16	64.71	TauPI-stichotoxin-Hcr2c OS=Heteractis crispa OX=17...	[more]
sp\|C0HK74\|VKT3_HETMG	2.593e-16	64.71	Kunitz-type serine protease inhibitor HMGS2 OS=Het...	[more]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3G1GLI4	1.878e-14	64.71	Kunitz-type peptide HCRG21 (Fragment) OS=Heteracti...	[more]
B7P626	3.044e-14	62.75	Colostrum trypsin inhibitor, putative (Fragment) O...	[more]
A0A3G1GLH1	5.798e-14	64.71	Kunitz-type peptide HMGS3c1 (Fragment) OS=Heteract...	[more]
A0A3G1GLH6	7.123e-14	64.71	Kunitz-type peptide HMGS3c4 (Fragment) OS=Heteract...	[more]
C3Z9Z8	7.415e-14	62.75	BPTI/Kunitz inhibitor domain-containing protein (F...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000015806.2	6.217e-13	58.33	tissue factor pathway inhibitor-like [Source:NCBI ...	[more]
appb	1.750e-12	47.06	amyloid beta A4 protein-like [Source:NCBI gene;Acc...	[more]
appb	1.919e-12	47.06	amyloid beta A4 protein-like [Source:NCBI gene;Acc...	[more]
ENSAMXT00000053501.1	2.578e-11	48.94	pep primary_assembly:Astyanax_mexicanus-2.0:12:313...	[more]
ENSAMXT00000002208.2	5.694e-11	42.31	pep primary_assembly:Astyanax_mexicanus-2.0:13:361...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tfpi2	3.181e-12	50.98	tissue factor pathway inhibitor 2 [Source:ZFIN;Acc...	[more]
ENSPMAT00000005252.1	3.185e-10	45.10	pep scaffold:Pmarinus_7.0:GL477257:283672:285029:-...	[more]
ENSPMAT00000008261.1	1.118e-9	41.51	pep scaffold:Pmarinus_7.0:GL478901:735:1448:1 gene...	[more]
WFIKKN1	1.127e-9	45.10	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
ENSPMAT00000005043.1	1.398e-9	43.40	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO29819	1.604e-16	54.72	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38284	2.371e-14	50.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41696	4.167e-14	50.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO26263	4.167e-14	50.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO31760	1.677e-12	45.28	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
APP	1.101e-11	45.10	amyloid-beta A4 protein [Source:NCBI gene;Acc:1011...	[more]
appb	1.170e-11	45.10	amyloid-beta A4 protein [Source:NCBI gene;Acc:1011...	[more]
ENSORLT00000012975.2	1.396e-11	50.00	pep primary_assembly:ASM223467v1:12:15740273:15760...	[more]
ENSORLT00000029029.1	1.944e-11	50.00	pep primary_assembly:ASM223467v1:5:3412877:3416364...	[more]
ENSORLT00000042019.1	2.214e-11	51.79	pep primary_assembly:ASM223467v1:12:15740119:15780...	[more]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000037999.1	5.934e-35	96.49	SMESG000037999.1	[more]
SMESG000037999.1	6.534e-35	96.49	SMESG000037999.1	[more]
SMESG000037995.1	7.303e-35	96.49	SMESG000037995.1	[more]
SMESG000037994.1	2.232e-22	64.91	SMESG000037994.1	[more]
SMESG000038052.1	1.479e-16	56.36	SMESG000038052.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30017992 ID=SMED30017992|Name=Kunitz-type protease inhibitor|organism=Schmidtea mediterranea sexual|type=transcript|length=431bp
TAAACTTTTGTTTTAATTATTTGTGTTAAATACATGTACATGAAGTCTAA
AATTCTAGTTTTAGTTGTATTATTCTCTTTGTCACAACTCTTGGAAAGTC
GGGTAGCCTTTTTTTGATTTTCTCCGATGTCAATTTTTTATTTATTTTTA
TTTAAAATTTGTTCATTATTTTACTTATTTATTATAATTATCTTTTTTAT
TTAAAGCTCAAGGAGTGTAATGAACCTAAACAGTCCGGAATGTGTAAGGC
ATATTTTAAAAGGTTCTATTTTAACAGTGAAACAGGAAAATGTGAAACGT
TTATTTATGGAGGATGTGGAAAAAATGGAAACAATTTTCAAACCGAAGAT
GAGTGTGAAGAATTGTGTATAGAATAAAAATATTTTTGGAAAATATTGTA
ATAAATTTTAATTTCGTTACTTTTATCGGAA

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000025	nervous system
PLANA:0000034	epidermis
PLANA:0000070	intestinal phagocyte
PLANA:0000099	neuron

Vocabulary: **INTERPRO**
Term	Definition
IPR002223	Kunitz_BPTI
IPR020901	Prtase_inh_Kunz-CS

Vocabulary: **molecular function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0030414	peptidase inhibitor activity

Vocabulary: **cellular component**
Term	Definition
GO:0005576	extracellular region

Vocabulary: **biological process**
Term	Definition
GO:0010466	negative regulation of peptidase activity
GO:0010951	negative regulation of endopeptidase activity

Main menu

Kunitz-type protease inhibitor

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Owlery

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