SMED30017988
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30017988 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 27
Alignments
SMED30017988 aligns in the following genomic locations:
Homology
BLAST of SMED30017988 vs. Planmine SMEST
Match: SMESG000064314.1 (SMESG000064314.1) HSP 1 Score: 284.648 bits (727), Expect = 3.187e-89 Identity = 147/165 (89.09%), Postives = 155/165 (93.94%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPE VSKVNEKDNSYDPNE LA++ I EIDQG+NNI D VN++E+SKVDE+ HSAD E+EPSE Sbjct: 3254 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNLNEISKVDEEDHSADLNENEPSE 3418 HSP 2 Score: 183.341 bits (464), Expect = 7.904e-54 Identity = 106/140 (75.71%), Postives = 112/140 (80.00%), Query Frame = 1 Query: 100 ADQIERE--PAESNINEAGEEEITQEK------FNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 D+I+R P ES + E EI Q K NL ++SKV EK S D NE+EPSE INEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE Sbjct: 2942 IDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 3081 HSP 3 Score: 138.272 bits (347), Expect = 4.462e-38 Identity = 99/198 (50.00%), Postives = 117/198 (59.09%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNE-------------------------------ETDHSADQIEREPAESNINEA--GEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 +NN+ DPV ++E S K S DLNE E D+S D E E A+ NI E GE I + N++++SKV EK S DS ESEPSE INEVDEGEPKQQEQ NPE V KVN+KDNS DPN+ EL + +I EIDQGENNIAD VNVDEVSKV+E H+ + ESEP E Sbjct: 2967 SKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIA-DSVNVDEVSKVDEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYE 3163 HSP 4 Score: 122.479 bits (306), Expect = 1.398e-32 Identity = 87/195 (44.62%), Postives = 110/195 (56.41%), Query Frame = 1 Query: 4 ENNVADPVYVDEGSNFYGKDDSGDLNE-------------------------------ETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 E N++DPV VDE S D + +LNE E D+S D EP +SNINE G + +T EKFNLE++SK E DDS D NE E + I +DEGE E+ N +SK++E D S PNE +LA+H I EIDQ +NNI D VN++E+SKVDEK HSAD E+EPSE Sbjct: 2806 EKNISDPVNVDEVSKVEETDRTANLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGK-MTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSE 2999 HSP 5 Score: 121.709 bits (304), Expect = 2.357e-32 Identity = 91/192 (47.40%), Postives = 112/192 (58.33%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNE-------------------------------ETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQ-VNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKE 480 G+NN+ DPV ++E S +D S DLNE E D+S D E EPAESNINE G E +T EKFNL + SK++EKDDS D N+SE +E IN VDE EPKQQ+Q N E SKV+EKDNS DPNE EL + +I I +GE NI++ NV E++KV K HS D E Sbjct: 3386 GKNNITDPVNLNEISKVDEEDHSADLNENEPSESNINEVDEGEPKQQEQVNPEGVSKVNEKDNSNDPSEIEPAESNINEFGGE-MTNEKFNLVEASKIFEKDDSFDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISEESNVKELTKVFGKQHSFDQIE 3576 HSP 6 Score: 110.923 bits (276), Expect = 1.475e-28 Identity = 88/207 (42.51%), Postives = 112/207 (54.11%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGE-EEITQEKFN-----------------------------------------LEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 GENN+AD V VDE S +E HSAD E EP+E NINE E E QE+FN ++++SKV E D +G+ NESEP ES I EVDEGEPKQQEQVNPE VSKVNEK +S D NE E+ + NI + +GE +I++ NV+E+SK+DE S + ES+ +E Sbjct: 3049 GENNIADSVNVDEVSKV----------DEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAE 3245 HSP 7 Score: 109.383 bits (272), Expect = 4.528e-28 Identity = 81/165 (49.09%), Postives = 104/165 (63.03%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 GE N++D V VD+ S KDD E+DHS D + EP +S INE E EIT +K NL+++ KV E +DS D NE E ES I V+EG+ E+ N S ++E D S DPN+ EL++H I EIDQ E NI+D VNVDEVSKV+E +A+ ESEP E Sbjct: 2684 GEKNISDKVNVDQVS----KDD------ESDHSVDPNKSEPGKSYINEI-EGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQVEKNISDPVNVDEVSKVEETDRTANLNESEPYE 2837 HSP 8 Score: 109.383 bits (272), Expect = 5.286e-28 Identity = 75/145 (51.72%), Postives = 100/145 (68.97%), Query Frame = 1 Query: 64 DSGDLNEETDHSADQIEREPAESNINEAGEEEITQEK-FNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 D ++ +ETDHSA+ + EP+ES INE E + Q+K +L+ +SKV +KD S + NESE +ES I+EVDE E KQ EQVN E VS V+ D+S +PNE ELA+ NI E+D + NI++ VN+DEVSKVDE D ESE +E Sbjct: 2123 DQVNVVDETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAE 2267 HSP 9 Score: 108.997 bits (271), Expect = 5.936e-28 Identity = 86/176 (48.86%), Postives = 102/176 (57.95%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGE-EEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKV----------DEKYHSADSKESEPSE 495 GENN+AD V VDE S EETDH+ + E EP ESNI E E E QE+ N E++SKV EK DSGD+NESE E I V EGE E+ N +SK++E D S NE +LA+H I EIDQGENN+AD V VDE S +E HSAD E EP+E Sbjct: 3131 GENNIADHVNVDEVSKV----------EETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAEHYIIEIDQGENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAE 3296 HSP 10 Score: 107.457 bits (267), Expect = 2.275e-27 Identity = 75/137 (54.74%), Postives = 93/137 (67.88%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 ET++S DQIERE AESNI+E EEEITQEKFNL+++SKV E D S D NESEPS S INE+ +GE ++VN + VSK +E D+S DPN+ E + I EI +GE +N+DEV KVDE S D E E E Sbjct: 2621 ETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEIT-HKKINLDEVPKVDENNDSVDPNEIEIVE 2755 HSP 11 Score: 105.531 bits (262), Expect = 1.110e-26 Identity = 76/162 (46.91%), Postives = 100/162 (61.73%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESE 486 GE N+++ ++E SN + D S DLNE EP ESNIN+ EEE TQE+ E++SKV EKD S D NES+ E I +VDE E KQ +Q+N + VSK++E D+S D NE + + NI E+D GE + VN +EVS VD+ YHS D ESE Sbjct: 1837 GERNISEQDNLEEVSNVFETDPSVDLNE----------NEPTESNINKVDEEETTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINLD-VSKLDETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESE 1987 HSP 12 Score: 92.4337 bits (228), Expect = 3.768e-22 Identity = 69/138 (50.00%), Postives = 87/138 (63.04%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEA-GEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 E D+S DQ E E + E E I ++ N E+ SK+ E D S D NESE +ES I+EVDEGE KQ EQVN E VS V+ D+S +PNE ELA+ NI +D + NI++ VN+DEVSKVDE D ESE +E Sbjct: 1404 ERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAE 1541 HSP 13 Score: 92.0485 bits (227), Expect = 5.874e-22 Identity = 67/137 (48.91%), Postives = 90/137 (65.69%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 ETDHS DQ + E A+ I E E EK L+ ++ V E D S +SN+SEPSES INEVDE + KQQ++V+ +GVSKV++KD S + NE ELA+ NI+E+D+ E + VN +EVS VD HS + E E +E Sbjct: 2094 ETDHSDDQNDGELAKQIIIEIDE----VEKNILDQVNVVDETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAE 2226 HSP 14 Score: 87.8113 bits (216), Expect = 1.475e-20 Identity = 63/136 (46.32%), Postives = 84/136 (61.76%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEA--GEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESE 486 +T HS DQ E E A NI + GE+ I L+ ++ +Y+ D S D N+ + +E INEVD GEPKQQEQVN + VSKV+ KD S D NE EL + N+ EID+ E NI++ NV E+ V+E HS D + E Sbjct: 1976 KTYHSVDQNESELANQNIIKIDKGEKNI------LDQVNVLYKTDHSVDPNKIKLAEFNINEVDNGEPKQQEQVNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNVKELLNVNETDHSDDQNDGE 2105 HSP 15 Score: 87.8113 bits (216), Expect = 1.640e-20 Identity = 66/135 (48.89%), Postives = 85/135 (62.96%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQ-EKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESE 486 ETDHS + EPAESNIN+ EEE Q E+ N +++ V KD S + +ESE E E+ E + E+ N + + KV E ++S D NE EL + NI EID+ E NI+D VNVDE+SKVDE HSAD ESE Sbjct: 2335 ETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESE 2469 HSP 16 Score: 87.0409 bits (214), Expect = 3.349e-20 Identity = 63/138 (45.65%), Postives = 88/138 (63.77%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQEKFNL-EDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 + DHS + E+ E E + E TQ + E+ SK+ EK S D NESEP+ES INEVD+ E KQQ++++ E VS V++ D+S D NE +LA+ NI E+D GE NI++ N++EVS V E S D E+EP+E Sbjct: 1732 KIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTE 1869 HSP 17 Score: 86.2705 bits (212), Expect = 5.219e-20 Identity = 60/130 (46.15%), Postives = 82/130 (63.08%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEI-TQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSAD 471 ET+HS DQ E E E NI E + E ++ N+++LSKV E D S D NESE + S INEV++ E KQQE VN + +S+V EKDNSY+PN+ E + NI +D+ E NI + + +S +D HS D Sbjct: 2417 ETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVD 2546 HSP 18 Score: 85.8853 bits (211), Expect = 7.038e-20 Identity = 72/176 (40.91%), Postives = 92/176 (52.27%), Query Frame = 1 Query: 94 HSADQIEREPAESNINEAGEEEITQEK-FNLEDLSKVYEKDDSGDSNES-----------------------------------------EPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 +SAD E EPAESNINE + E Q+K +LE++S V + D S DSNES EP+ES IN+VDE E QEQ+ E VSKV EKD S DPNE + +HNI ++D+ E +N+D VSK+DE HS D+ ES+P E Sbjct: 1776 YSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDEEE-TTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINLD-VSKLDETDHSVDTNESKPVE 1949 HSP 19 Score: 84.3445 bits (207), Expect = 2.742e-19 Identity = 74/167 (44.31%), Postives = 95/167 (56.89%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEA--GEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 GE N+ D V V Y +TDHS D + + AE NINE GE + QE+ N +++SKV KD S D+NESE E + E+DE E E+ N + + VNE D+S D N+ ELA+ I EID+ E NI D VNV VDE HSA+S +SEPSE Sbjct: 1999 GEKNILDQVNV-----LY----------KTDHSVDPNKIKLAEFNINEVDNGEPK-QQEQVNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNVKELLNVNETDHSDDQNDGELAKQIIIEIDEVEKNILDQVNV-----VDETDHSANSNKSEPSE 2144 HSP 20 Score: 82.4185 bits (202), Expect = 1.345e-18 Identity = 68/137 (49.64%), Postives = 82/137 (59.85%), Query Frame = 1 Query: 88 TDHSADQIEREPAESNINEAG-EEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 TDHS + E E AE NI E ++ EK NL+++SKV E D D NESE +ES IN+VDEGE KQQEQVN E VS V E +NS NE L + E+D EN N +E SKVDE HS +SEP+E Sbjct: 2213 TDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAE 2349 HSP 21 Score: 80.4925 bits (197), Expect = 6.289e-18 Identity = 71/167 (42.51%), Postives = 93/167 (55.69%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAES--NINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 GE + V +E SN Y ++S LNE T + + E + E+ N++ EEE SKV E D S N+SEP+ES IN+VDE E KQQ+QVN E V +V EK NS DPN E N+ +D+ E NI + NV +S VDEK HS D +SE +E Sbjct: 1550 GETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEE-----------SKVDETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNVKSLSNVDEKEHSTDQIKSELAE 1705 HSP 22 Score: 80.1073 bits (196), Expect = 6.793e-18 Identity = 68/138 (49.28%), Postives = 83/138 (60.14%), Query Frame = 1 Query: 88 TDHSADQIEREPAESNI--NEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 TDHS + E E AE NI + + I+ EK NL+++SKV E D D NESE +ES IN+VDEGE KQQEQVN E VS V E +NS NE L + E+D EN N +E SKVDE HS +SEP+E Sbjct: 1487 TDHSGEPNEHELAEQNIIGVDVDKRNIS-EKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAE 1623 HSP 23 Score: 78.1814 bits (191), Expect = 3.667e-17 Identity = 60/121 (49.59%), Postives = 75/121 (61.98%), Query Frame = 1 Query: 154 EEITQEKFNLEDLSKVYEKDD---------SGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEP 489 E IT+E N+E++ Y K + S D E E +ES I+EVDE E QE+ N + VSKV+E D+S DPNE E + NI EI QGE NI+D VNVD+VSK DE HS D +SEP Sbjct: 2595 EPITKEVDNIENIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEI-TQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEP 2714 HSP 24 Score: 77.7962 bits (190), Expect = 4.620e-17 Identity = 61/139 (43.88%), Postives = 84/139 (60.43%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEE--ITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 E +HS DQI+ E AE NI EEE IT + NL++ SKV + D S + NE +E E+D+ E Q + V E SK++EK S DPNE E A+ NI E+D+ E ++++EVS VD+ HSADS ES+ +E Sbjct: 1691 EKEHSTDQIKSELAEPNIRNVDEEERNITADA-NLKEESKVDKIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAE 1828 HSP 25 Score: 73.9442 bits (180), Expect = 1.076e-15 Identity = 55/126 (43.65%), Postives = 77/126 (61.11%), Query Frame = 1 Query: 118 EPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 E A+ NI + EE QE+ NLE+++ V E+D S D NE +E + EV E Q ++VN E SK++E D+S D NE ELA+ NI+E+D+GE + VN +EVS VD HS + E E +E Sbjct: 1375 ELADHNIIKVEEETKKQEQINLEEVTNVDERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAE 1500 HSP 26 Score: 73.9442 bits (180), Expect = 1.140e-15 Identity = 58/122 (47.54%), Postives = 74/122 (60.66%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQ-EKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKV 447 ETDHS D E E AESNI+E E E Q E+ N E++S V D SG+ NE E +E I VD + E+VN + VSKV+E D DPNE E A+ NI ++D+GE + VN +EVS V Sbjct: 1445 ETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNV 1566 HSP 27 Score: 71.2478 bits (173), Expect = 1.041e-14 Identity = 60/155 (38.71%), Postives = 88/155 (56.77%), Query Frame = 1 Query: 31 VDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 V+E N Y ++ D+S D E E+ IN+ EE I Q++ NLE++ KV E ++ + N SE ++ I +V+E E K+QEQ+N E V+ V+E+D S D NE L + + E+ EN VN +E SK+DE HS D ESE +E Sbjct: 1310 VNETFNLYVMTKM----DQKDNSVDPYESAHIEAKINKIDEETIKQKQVNLEEVPKVDETEEFINPNRSELADHNIIKVEE-ETKKQEQINLEEVTNVDERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAE 1459 HSP 28 Score: 68.9366 bits (167), Expect = 6.467e-14 Identity = 57/138 (41.30%), Postives = 80/138 (57.97%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQE-KFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 ETDHS + EPAESNIN+ EEE Q+ + N E + +V EK +S D N E + + VDE E E+ N + +S V+EK++S D + ELA+ NI +D+ E NI N+ E SKVD+ HS + E +E Sbjct: 1609 ETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNVKSLSNVDEKEHSTDQIKSELAEPNIRNVDEEERNITADANLKEESKVDKIDHSVNLNEKTLTE 1746 HSP 29 Score: 68.5514 bits (166), Expect = 6.984e-14 Identity = 66/166 (39.76%), Postives = 96/166 (57.83%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKF-NLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSE 495 E N++D V VDE S +E DHSAD E E A SNINE ++E Q++ N ++LS+VYEKD+S + N+ E E I VDE E E+ + +S ++ +S D NE ELA+ NI +++ E ++ + N+ EVSKVDE Y+S D E+ +E Sbjct: 2440 SEKNISDQVNVDELSKV----------DEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTE 2595 HSP 30 Score: 63.1586 bits (152), Expect = 6.478e-12 Identity = 47/121 (38.84%), Postives = 70/121 (57.85%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKV 447 +T+HS +Q++ + E NI + NL DLS+V E D S +SNE + + EVD+G+ Q E N E +SKV+E +S +PNE EL + NI E+D+GE N VN +E + + Sbjct: 3674 KTEHSVNQMKCKIEERNITDDA---------NLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVNPNESTPI 3785 HSP 31 Score: 62.7734 bits (151), Expect = 9.060e-12 Identity = 60/157 (38.22%), Postives = 82/157 (52.23%), Query Frame = 1 Query: 1 GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSAD 471 GE N+++ V E + +GK HS DQIE E NI + N +D+S V E D + DSNESEP+ES NEVDE E +E+ SKV+EKDNS DP + EL + I + GE NI+ + ++ KVD+ HS + Sbjct: 3549 GEKNISEESNVKELTKVFGKQ----------HSFDQIEGNSEERNITD---------DVNYKDVSHVDETDHADDSNESEPAESNFNEVDEEEGNSEEE------SKVDEKDNSDDPYKNELVEPLIKNFNVGEKNISGESCLKDLLKVDKTEHSVN 3680 HSP 32 Score: 60.8474 bits (146), Expect = 3.599e-11 Identity = 56/134 (41.79%), Postives = 70/134 (52.24%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQ-EKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKES 483 E DHS + ER N+ E + + Q E NLE++SKV E S + NESE ES I EVDEGE QQ+QVNP N P EC N E+D GE + VN++E S VD+ + D KES Sbjct: 3705 EIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVNP----------NESTPIEC-----NTNEVDGGEKYQTEHVNLEEESMVDKIDNFVDPKES 3823 HSP 33 Score: 48.9062 bits (115), Expect = 5.241e-7 Identity = 47/132 (35.61%), Postives = 64/132 (48.48%), Query Frame = 1 Query: 85 ETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKE 480 E HSA+ E E ESNI E E E Q+K + NES P E NEVD GE Q E VN E S V++ DN DP E + ++ ++ +GE N A++ + + + VD + D KE Sbjct: 3746 EIHHSANPNESELDESNIYEVDEGETNQQK--------------QVNPNESTPIECNTNEVDGGEKYQTEHVNLEEESMVDKIDNFVDPKESPFTKPDVNKVSEGEKNKAET-QIVKSTDVDTIFVQNDVKE 3862 The following BLAST results are available for this feature:
BLAST of SMED30017988 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30017988 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30017988 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30017988 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30017988 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30017988 ID=SMED30017988|Name=SMED30017988|organism=Schmidtea mediterranea sexual|type=transcript|length=496bpback to top protein sequence of SMED30017988-orf-1 >SMED30017988-orf-1 ID=SMED30017988-orf-1|Name=SMED30017988-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=165bp GENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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