SMED30017899

Overview


Name	SMED30017899
Smed ID	SMED30017899
Length (bp)	386

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30017899 (SMED30017899) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017899 (SMED30017899) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017899 (SMED30017899) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017899 (SMED30017899) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30017899

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 18

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30017899	h1SMcG0009729	Contig7552	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30017899	h1SMcG0009729	Contig7552	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig47447	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig47447	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig17060	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig17060	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig16537	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig16537	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig45798	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig45798	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig35011	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899	h1SMcG0009729	Contig35011	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899		Contig16094	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017899		Contig16094	newmark_ests	PMID:29674431 Fincher et al., 2018
oocyte	SMED30017899	h1SMcG0009729	Contig36008	newmark_ests	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30017899	h1SMcG0009729	Contig36008	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30017899	h1SMcG0009729	Contig36008	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30017899	h1SMcG0009729	Contig36008	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30017899 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.019091:4809..5194 +	1759

Homology

[Close]

BLAST of SMED30017899 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 47.3654 bits (111), Expect = 6.192e-7
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  111 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 175

[Close]

BLAST of SMED30017899 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 47.3654 bits (111), Expect = 6.714e-7
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  109 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 173

HSP 2 Score: 46.595 bits (109), Expect = 1.129e-6
Identity = 24/59 (40.68%), Postives = 33/59 (55.93%), Query Frame = 2

Query:  104 CSTCQRIGHRSENC-FRNYTCQKCGRRGHTEKICRQPN---NKACYNCGQQGHFARQCN 268
            C  C   GH +++C  +   C  CGR GH  K C++P     + CYNCG+ GH AR C+
Sbjct:   54 CYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCD 112

[Close]

BLAST of SMED30017899 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 47.3654 bits (111), Expect = 6.831e-7
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  104 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 168

[Close]

BLAST of SMED30017899 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 47.3654 bits (111), Expect = 7.286e-7
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  110 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 174

HSP 2 Score: 45.4394 bits (106), Expect = 3.342e-6
Identity = 24/60 (40.00%), Postives = 33/60 (55.00%), Query Frame = 2

Query:  104 CSTCQRIGHRSENCF--RNYTCQKCGRRGHTEKICRQPN---NKACYNCGQQGHFARQCN 268
            C  C   GH +++C    +  C  CGR GH  K C++P     + CYNCG+ GH AR C+
Sbjct:   54 CYRCGESGHLAKDCDLQEDEACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCD 113

[Close]

BLAST of SMED30017899 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 46.9802 bits (110), Expect = 7.866e-7
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  102 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 166

HSP 2 Score: 46.595 bits (109), Expect = 1.258e-6
Identity = 24/59 (40.68%), Postives = 33/59 (55.93%), Query Frame = 2

Query:  104 CSTCQRIGHRSENC-FRNYTCQKCGRRGHTEKICRQPN---NKACYNCGQQGHFARQCN 268
            C  C   GH +++C  +   C  CGR GH  K C++P     + CYNCG+ GH AR C+
Sbjct:   47 CYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCD 105

[Close]

BLAST of SMED30017899 vs. Ensembl Celegans
Match: glh-4 (ATP-dependent RNA helicase glh-4 [Source:UniProtKB/Swiss-Prot;Acc:O76743])

HSP 1 Score: 53.1434 bits (126), Expect = 5.426e-9
Identity = 26/71 (36.62%), Postives = 37/71 (52.11%), Query Frame = 2

Query:  104 CSTCQRIGHRSENCFRNYT----CQKCGRRGHTEKICRQPN--NKACYNCGQQGHFARQCN---IRREPNQ 289
            C  C+++GH + +C +       C+ CG  GH    C QP      C NCGQ+GHFA+ C    +R EP +
Sbjct:  595 CRNCEQLGHFASDCDQPRVPRGPCRNCGIEGHFAVDCDQPKVPRGPCRNCGQEGHFAKDCQNERVRMEPTE 665

HSP 2 Score: 47.7506 bits (112), Expect = 4.189e-7
Identity = 29/89 (32.58%), Postives = 41/89 (46.07%), Query Frame = 2

Query:   44 GNNQVSYNNNFRSNPNANEK---CSTCQRIGHRSENCFR----NYTCQKCGRRGHTEKICRQPN--NKACYNCGQQGHFARQCNIRREP 283
            GN     ++N R N +  E+   C  C   GH S+ C +     + C+ C + GH    C QP      C NCG +GHFA  C+  + P
Sbjct:  549 GNTFGEPSDNQRGNWDGGERPRGCHNCGEEGHISKECDKPKVPRFPCRNCEQLGHFASDCDQPRVPRGPCRNCGIEGHFAVDCDQPKVP 637

[Close]

Query:  104 CSTCQRIGHRSENCFRNYT----CQKCGRRGHTEKICRQPN--NKACYNCGQQGHFARQCN---IRREPNQ 289
            C  C+++GH + +C +       C+ CG  GH    C QP      C NCGQ+GHFA+ C    +R EP +
Sbjct:  595 CRNCEQLGHFASDCDQPRVPRGPCRNCGIEGHFAVDCDQPKVPRGPCRNCGQEGHFAKDCQNERVRMEPTE 665

HSP 2 Score: 47.7506 bits (112), Expect = 4.189e-7
Identity = 29/89 (32.58%), Postives = 41/89 (46.07%), Query Frame = 2

Query:   44 GNNQVSYNNNFRSNPNANEK---CSTCQRIGHRSENCFR----NYTCQKCGRRGHTEKICRQPN--NKACYNCGQQGHFARQCNIRREP 283
            GN     ++N R N +  E+   C  C   GH S+ C +     + C+ C + GH    C QP      C NCG +GHFA  C+  + P
Sbjct:  549 GNTFGEPSDNQRGNWDGGERPRGCHNCGEEGHISKECDKPKVPRFPCRNCEQLGHFASDCDQPRVPRGPCRNCGIEGHFAVDCDQPKVP 637

[Close]

BLAST of SMED30017899 vs. Ensembl Fly
Match: CNBP (gene:FBgn0034802 transcript:FBtr0303464)

HSP 1 Score: 43.1282 bits (100), Expect = 9.097e-6
Identity = 26/71 (36.62%), Postives = 38/71 (53.52%), Query Frame = 2

Query:   77 RSNPNANEKCSTCQRIGHRSENCFR--NYTCQKCGRRGHTEKIC------RQPNNKACYNCGQQGHFARQC 265
            R+ P   E+C  C  IGH S++C +  N TC +C + GH  + C      R P N +CY C + GH ++ C
Sbjct:   68 RACPEEAERCYRCNGIGHISKDCTQADNPTCYRCNKTGHWVRNCPEAVNERGPTNVSCYKCNRTGHISKNC 138

[Close]

BLAST of SMED30017899 vs. Ensembl Fly
Match: CNBP (gene:FBgn0034802 transcript:FBtr0071994)

HSP 1 Score: 43.1282 bits (100), Expect = 9.097e-6
Identity = 26/71 (36.62%), Postives = 38/71 (53.52%), Query Frame = 2

Query:   77 RSNPNANEKCSTCQRIGHRSENCFR--NYTCQKCGRRGHTEKIC------RQPNNKACYNCGQQGHFARQC 265
            R+ P   E+C  C  IGH S++C +  N TC +C + GH  + C      R P N +CY C + GH ++ C
Sbjct:   68 RACPEEAERCYRCNGIGHISKDCTQADNPTCYRCNKTGHWVRNCPEAVNERGPTNVSCYKCNRTGHISKNC 138

[Close]

BLAST of SMED30017899 vs. Ensembl Zebrafish
Match: cnbpb (CCHC-type zinc finger, nucleic acid binding protein b [Source:ZFIN;Acc:ZDB-GENE-030131-7782])

HSP 1 Score: 48.1358 bits (113), Expect = 1.994e-7
Identity = 30/79 (37.97%), Postives = 40/79 (50.63%), Query Frame = 2

Query:   53 QVSYN-----NNFRSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            QV YN     +  R   +ANE KC +C   GH  + C     C +CG  GH    C + +   CYNCG+ GH A++C I
Sbjct:   80 QVCYNCGKAGHMARDCDHANEQKCYSCGGFGHIQKGC-EKVKCYRCGEIGHVAVQCSKASEVNCYNCGKSGHVAKECTI 157

HSP 2 Score: 44.669 bits (104), Expect = 3.940e-6
Identity = 24/59 (40.68%), Postives = 32/59 (54.24%), Query Frame = 2

Query:  104 CSTCQRIGHRSENCFRNY-TCQKCGRRGHTEKICRQPNNK---ACYNCGQQGHFARQCN 268
            C  C   GH + +C R    C  CGR GH  + C++P  +    CYNCG+ GH AR C+
Sbjct:   38 CYRCGEPGHVARDCERTEDACYNCGRGGHISRDCKEPKKEREQVCYNCGKAGHMARDCD 96

[Close]

Query:   53 QVSYN-----NNFRSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            QV YN     +  R   +ANE KC +C   GH  + C     C +CG  GH    C + +   CYNCG+ GH A++C I
Sbjct:   80 QVCYNCGKAGHMARDCDHANEQKCYSCGGFGHIQKGC-EKVKCYRCGEIGHVAVQCSKASEVNCYNCGKSGHVAKECTI 157

HSP 2 Score: 44.669 bits (104), Expect = 3.940e-6
Identity = 24/59 (40.68%), Postives = 32/59 (54.24%), Query Frame = 2

Query:  104 CSTCQRIGHRSENCFRNY-TCQKCGRRGHTEKICRQPNNK---ACYNCGQQGHFARQCN 268
            C  C   GH + +C R    C  CGR GH  + C++P  +    CYNCG+ GH AR C+
Sbjct:   38 CYRCGEPGHVARDCERTEDACYNCGRGGHISRDCKEPKKEREQVCYNCGKAGHMARDCD 96

[Close]

BLAST of SMED30017899 vs. Ensembl Zebrafish
Match: cnbpa (CCHC-type zinc finger, nucleic acid binding protein a [Source:ZFIN;Acc:ZDB-GENE-030131-5045])

HSP 1 Score: 46.595 bits (109), Expect = 6.667e-7
Identity = 27/66 (40.91%), Postives = 35/66 (53.03%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +ANE KC +C   GH  + C +   C +CG  GH    C +     CYNCG+ GH AR C+I
Sbjct:   95 RDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVQCSKATEVNCYNCGKTGHLARDCSI 159

[Close]

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +ANE KC +C   GH  + C +   C +CG  GH    C +     CYNCG+ GH AR C+I
Sbjct:   95 RDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVQCSKATEVNCYNCGKTGHLARDCSI 159

[Close]

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +ANE KC +C   GH  + C +   C +CG  GH    C +     CYNCG+ GH AR C+I
Sbjct:   95 RDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVQCSKATEVNCYNCGKTGHLARDCSI 159

[Close]

BLAST of SMED30017899 vs. Ensembl Mouse
Match: Zcchc13 (zinc finger, CCHC domain containing 13 [Source:MGI Symbol;Acc:MGI:1922314])

HSP 1 Score: 51.2174 bits (121), Expect = 1.673e-8
Identity = 33/90 (36.67%), Postives = 43/90 (47.78%), Query Frame = 2

Query:   80 SNPNANEKCSTCQRIGHRSENC-FRNYTCQKCGRRGHTEKICRQPN---NKACYNCGQQGHFARQCNIRREPNQSRLNNSMGNPQINVMQ 337
            S  N ++ C  C   GH +++C     TC  CGRRGH  K C Q      + CY C Q GH AR CN R+E  +       G+ Q +  Q
Sbjct:   39 STANQSDVCYRCGETGHYAKDCDLLQDTCYNCGRRGHIAKDCTQAKREREQCCYICSQPGHLARDCN-RQEEQKCYTCGEFGHIQKDCTQ 127

HSP 2 Score: 46.2098 bits (108), Expect = 1.126e-6
Identity = 24/63 (38.10%), Postives = 34/63 (53.97%), Query Frame = 2

Query:   83 NPNANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            N    +KC TC   GH  ++C +   C +CG  GH    C + +  +CY CG+ GH AR+C I
Sbjct:  105 NRQEEQKCYTCGEFGHIQKDCTQ-IKCYRCGENGHMAVNCSKTSEVSCYRCGESGHLARECPI 166

[Close]

BLAST of SMED30017899 vs. Ensembl Mouse
Match: Cnbp (cellular nucleic acid binding protein [Source:MGI Symbol;Acc:MGI:88431])

HSP 1 Score: 47.3654 bits (111), Expect = 4.609e-7
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  109 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 173

HSP 2 Score: 46.595 bits (109), Expect = 7.748e-7
Identity = 24/59 (40.68%), Postives = 33/59 (55.93%), Query Frame = 2

Query:  104 CSTCQRIGHRSENC-FRNYTCQKCGRRGHTEKICRQPN---NKACYNCGQQGHFARQCN 268
            C  C   GH +++C  +   C  CGR GH  K C++P     + CYNCG+ GH AR C+
Sbjct:   54 CYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCD 112

[Close]

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  109 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 173

HSP 2 Score: 46.595 bits (109), Expect = 7.748e-7
Identity = 24/59 (40.68%), Postives = 33/59 (55.93%), Query Frame = 2

Query:  104 CSTCQRIGHRSENC-FRNYTCQKCGRRGHTEKICRQPN---NKACYNCGQQGHFARQCN 268
            C  C   GH +++C  +   C  CGR GH  K C++P     + CYNCG+ GH AR C+
Sbjct:   54 CYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCD 112

[Close]

BLAST of SMED30017899 vs. Ensembl Mouse
Match: Cnbp (cellular nucleic acid binding protein [Source:MGI Symbol;Acc:MGI:88431])

HSP 1 Score: 47.3654 bits (111), Expect = 5.002e-7
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  110 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 174

HSP 2 Score: 45.4394 bits (106), Expect = 2.295e-6
Identity = 24/60 (40.00%), Postives = 33/60 (55.00%), Query Frame = 2

Query:  104 CSTCQRIGHRSENCF--RNYTCQKCGRRGHTEKICRQPN---NKACYNCGQQGHFARQCN 268
            C  C   GH +++C    +  C  CGR GH  K C++P     + CYNCG+ GH AR C+
Sbjct:   54 CYRCGESGHLAKDCDLQEDEACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCD 113

[Close]

BLAST of SMED30017899 vs. Ensembl Mouse
Match: Cnbp (cellular nucleic acid binding protein [Source:MGI Symbol;Acc:MGI:88431])

HSP 1 Score: 46.9802 bits (110), Expect = 5.400e-7
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +A+E KC +C   GH  ++C +   C +CG  GH    C + +   CY CG+ GH AR+C I
Sbjct:  102 RDCDHADEQKCYSCGEFGHIQKDCTK-VKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTI 166

HSP 2 Score: 46.595 bits (109), Expect = 8.639e-7
Identity = 24/59 (40.68%), Postives = 33/59 (55.93%), Query Frame = 2

Query:  104 CSTCQRIGHRSENC-FRNYTCQKCGRRGHTEKICRQPN---NKACYNCGQQGHFARQCN 268
            C  C   GH +++C  +   C  CGR GH  K C++P     + CYNCG+ GH AR C+
Sbjct:   47 CYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCD 105

[Close]

BLAST of SMED30017899 vs. UniProt/SwissProt
Match: sp|O65639|CSP1_ARATH (Cold shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=CSP1 PE=1 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 4.126e-8
Identity = 27/77 (35.06%), Postives = 40/77 (51.95%), Query Frame = 2

Query:   68 NNFRSNPNANEKCSTCQRIGHRSENCFR----NYTCQKCGRRGHTEKICRQ------PNNKACYNCGQQGHFARQCN 268
             N RS    +  C +C  +GH + +C      +  C +CG  GH  + C Q       N+ ACY CG++GHFAR+C+
Sbjct:  220 GNVRSGGGGSGTCYSCGGVGHIARDCATKRQPSRGCYQCGGSGHLARDCDQRGSGGGGNDNACYKCGKEGHFARECS 296

HSP 2 Score: 47.3654 bits (111), Expect = 6.740e-6
Identity = 27/78 (34.62%), Postives = 36/78 (46.15%), Query Frame = 2

Query:   89 NANEKCSTCQRIGHRSENCFRNY-------------TCQKCGRRGHTEKIC---RQPNNKACYNCGQQGHFARQCNIR 274
              N+ C TC  +GH + +C +               TC  CG  GH  + C   RQP+ + CY CG  GH AR C+ R
Sbjct:  195 GGNDGCYTCGDVGHFARDCTQKVAAGNVRSGGGGSGTCYSCGGVGHIARDCATKRQPS-RGCYQCGGSGHLARDCDQR 271

[Close]

BLAST of SMED30017899 vs. UniProt/SwissProt
Match: sp|O76743|GLH4_CAEEL (ATP-dependent RNA helicase glh-4 OS=Caenorhabditis elegans OX=6239 GN=glh-4 PE=1 SV=2)

HSP 1 Score: 53.1434 bits (126), Expect = 6.901e-8
Identity = 26/71 (36.62%), Postives = 37/71 (52.11%), Query Frame = 2

Query:  104 CSTCQRIGHRSENCFRNYT----CQKCGRRGHTEKICRQPN--NKACYNCGQQGHFARQCN---IRREPNQ 289
            C  C+++GH + +C +       C+ CG  GH    C QP      C NCGQ+GHFA+ C    +R EP +
Sbjct:  595 CRNCEQLGHFASDCDQPRVPRGPCRNCGIEGHFAVDCDQPKVPRGPCRNCGQEGHFAKDCQNERVRMEPTE 665

HSP 2 Score: 47.7506 bits (112), Expect = 5.328e-6
Identity = 29/89 (32.58%), Postives = 41/89 (46.07%), Query Frame = 2

Query:   44 GNNQVSYNNNFRSNPNANEK---CSTCQRIGHRSENCFR----NYTCQKCGRRGHTEKICRQPN--NKACYNCGQQGHFARQCNIRREP 283
            GN     ++N R N +  E+   C  C   GH S+ C +     + C+ C + GH    C QP      C NCG +GHFA  C+  + P
Sbjct:  549 GNTFGEPSDNQRGNWDGGERPRGCHNCGEEGHISKECDKPKVPRFPCRNCEQLGHFASDCDQPRVPRGPCRNCGIEGHFAVDCDQPKVP 637

[Close]

BLAST of SMED30017899 vs. UniProt/SwissProt
Match: sp|Q89928|POL_HV2EH (Gag-Pol polyprotein OS=Human immunodeficiency virus type 2 subtype B (isolate EHO) OX=388821 GN=gag-pol PE=3 SV=3)

HSP 1 Score: 53.1434 bits (126), Expect = 7.778e-8
Identity = 19/43 (44.19%), Postives = 26/43 (60.47%), Query Frame = 2

Query:  149 RNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRR 277
            R  TC  CG+ GHT + C+ P  + C+ CGQQGH   +C  R+
Sbjct:  385 RTVTCWNCGKAGHTARQCKAPRRQGCWKCGQQGHIMSKCPERQ 427

[Close]

BLAST of SMED30017899 vs. UniProt/SwissProt
Match: sp|Q9Q720|POL_HV1V9 (Gag-Pol polyprotein OS=Human immunodeficiency virus type 1 group M subtype H (isolate VI991) OX=388888 GN=gag-pol PE=3 SV=3)

HSP 1 Score: 51.9878 bits (123), Expect = 1.700e-7
Identity = 18/43 (41.86%), Postives = 24/43 (55.81%), Query Frame = 2

Query:  149 RNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRR 277
            R   C  CG+ GH  + CR P  K C+ CGQ+GH  + C  R+
Sbjct:  391 RTVKCSNCGKEGHIARNCRAPRKKGCWKCGQEGHQMKDCTGRQ 433

[Close]

BLAST of SMED30017899 vs. UniProt/SwissProt
Match: sp|Q76634|POL_HV2UC (Gag-Pol polyprotein OS=Human immunodeficiency virus type 2 subtype B (isolate UC1) OX=388822 GN=gag-pol PE=3 SV=3)

HSP 1 Score: 51.9878 bits (123), Expect = 1.786e-7
Identity = 19/40 (47.50%), Postives = 25/40 (62.50%), Query Frame = 2

Query:  158 TCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRR 277
            TC  CG+ GHT K CR P  + C+ CG+QGH   +C  R+
Sbjct:  389 TCWNCGKVGHTAKQCRAPRRQGCWKCGKQGHIMSKCPERQ 428

[Close]

BLAST of SMED30017899 vs. TrEMBL
Match: A0A261Y734 (Uncharacterized protein OS=Bifiguratus adelaidae OX=1938954 GN=BZG36_00744 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.445e-10
Identity = 28/55 (50.91%), Postives = 33/55 (60.00%), Query Frame = 2

Query:  104 CSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCN 268
            C TC + GHR+ NC     C  CG+ GH    C QP  KACY CGQ GH +R+CN
Sbjct:   69 CYTCGQTGHRAANCPNGQKCYNCGQTGHISSQCSQPQTKACYQCGQPGHISRECN 123

[Close]

BLAST of SMED30017899 vs. TrEMBL
Match: A0A1R0H6H2 (DNA-binding protein HEXBP OS=Smittium mucronatum OX=133383 GN=AYI68_g1092 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.792e-9
Identity = 29/68 (42.65%), Postives = 40/68 (58.82%), Query Frame = 2

Query:   62 YNNNFRSNPNANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQC 265
            YN N R   N+ E C +C  +GHRS +C     C  CG  GH    C++ +NK+CYNCG+ GH ++ C
Sbjct:  146 YNRNDRGGNNSRESCFSCGEVGHRSRDCPNGTKCYNCGEFGHMSSDCKKASNKSCYNCGEAGHTSKAC 213

[Close]

BLAST of SMED30017899 vs. TrEMBL
Match: A0A396HR19 (Putative nucleotidyltransferase, Ribonuclease H OS=Medicago truncatula OX=3880 GN=MtrunA17_Chr5g0422121 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 8.089e-9
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 2

Query:   80 SNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQ-PNNKACYNCGQQGHFARQC 265
            S P+  E KC  C  +GH + +C  ++TC KCG+ GH    CR       CYNCG++GH + +C
Sbjct:  268 SRPSLAEVKCFKCGAMGHYASDCKNDFTCHKCGKAGHKAVDCRGVARETTCYNCGEKGHISTKC 331

[Close]

BLAST of SMED30017899 vs. TrEMBL
Match: A0A146I6E9 (Uncharacterized protein OS=Mycena chlorophos OX=658473 GN=MCHLO_12297 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.445e-8
Identity = 23/58 (39.66%), Postives = 33/58 (56.90%), Query Frame = 2

Query:   92 ANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQC 265
            +++ C TC  +GH S +C +   C  CG  GH  + C Q   +ACY CG +GH +R C
Sbjct: 1347 SSKTCYTCGGVGHLSRDCVQGSKCYNCGEIGHISRDCPQAQRRACYTCGSEGHISRDC 1404

[Close]

BLAST of SMED30017899 vs. TrEMBL
Match: A0A146I6C1 (Uncharacterized protein OS=Mycena chlorophos OX=658473 GN=MCHLO_12297 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.446e-8
Identity = 23/58 (39.66%), Postives = 33/58 (56.90%), Query Frame = 2

Query:   92 ANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQC 265
            +++ C TC  +GH S +C +   C  CG  GH  + C Q   +ACY CG +GH +R C
Sbjct: 1360 SSKTCYTCGGVGHLSRDCVQGSKCYNCGEIGHISRDCPQAQRRACYTCGSEGHISRDC 1417

[Close]

BLAST of SMED30017899 vs. Ensembl Cavefish
Match: ENSAMXT00000005562.2 (cellular nucleic acid-binding protein [Source:NCBI gene;Acc:103027392])

HSP 1 Score: 48.521 bits (114), Expect = 1.119e-7
Identity = 30/79 (37.97%), Postives = 40/79 (50.63%), Query Frame = 2

Query:   53 QVSYN-----NNFRSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            QV YN     +  R   +ANE KC +C   GH  + C     C +CG  GH    C + +   CYNCG+ GH A++C I
Sbjct:   80 QVCYNCGKAGHVARDCDHANEQKCYSCGGFGHIQKGC-EKVKCYRCGEIGHVAVQCSKASEVNCYNCGKSGHLAKECTI 157

HSP 2 Score: 44.669 bits (104), Expect = 3.339e-6
Identity = 24/59 (40.68%), Postives = 32/59 (54.24%), Query Frame = 2

Query:  104 CSTCQRIGHRSENCFRNY-TCQKCGRRGHTEKICRQPNNK---ACYNCGQQGHFARQCN 268
            C  C   GH + +C R    C  CGR GH  + C++P  +    CYNCG+ GH AR C+
Sbjct:   38 CYRCGEPGHVARDCERTEDACYNCGRGGHISRDCKEPKKEREQVCYNCGKAGHVARDCD 96

[Close]

BLAST of SMED30017899 vs. Ensembl Cavefish
Match: cnbpa (cellular nucleic acid-binding protein-like [Source:NCBI gene;Acc:103024401])

HSP 1 Score: 47.7506 bits (112), Expect = 2.523e-7
Identity = 27/66 (40.91%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +ANE KC +C   GH  + C +   C +CG  GH    C + +   CYNCG+ GH AR+C I
Sbjct:  104 RDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVQCSKASEVNCYNCGKTGHLARECTI 168

[Close]

BLAST of SMED30017899 vs. Ensembl Cavefish
Match: ENSAMXT00000033510.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000951.1:16495:20219:1 gene:ENSAMXG00000033087.1 transcript:ENSAMXT00000033510.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.4394 bits (106), Expect = 3.304e-6
Identity = 28/97 (28.87%), Postives = 39/97 (40.21%), Query Frame = 2

Query:   44 GNNQVSYNNNFRSNPNANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRREPNQSRLNNS-MGNPQINV 331
            G+  +  +  F   P     C  C   GH  E C     C+ CG  GH    CR P  K+C  CG + H  R C  +  P  S +    +G P  ++
Sbjct:  157 GSFAIGPHQGFLHYPGQPLYCRRCGGPGHTKEAC-TGLRCRFCGEEGHVAANCRAP--KSCSLCGSRDHLFRDCPEKSAPAASVVTGGPVGRPSPSL 250

[Close]

BLAST of SMED30017899 vs. Ensembl Yeast
Match: GIS2 (Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 [Source:SGD;Acc:S000005199])

HSP 1 Score: 48.521 bits (114), Expect = 1.260e-8
Identity = 23/65 (35.38%), Postives = 34/65 (52.31%), Query Frame = 2

Query:   92 ANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNN---KACYNCGQQGHFARQCNIRR 277
            + + C  C +IGH +E+C     C  C + GH +  C  P     K CYNCG+ GH   +C ++R
Sbjct:    2 SQKACYVCGKIGHLAEDCDSERLCYNCNKPGHVQTDCTMPRTVEFKQCYNCGETGHVRSECTVQR 66

[Close]

BLAST of SMED30017899 vs. Ensembl Medaka
Match: ENSORLT00000043369.1 (pep primary_assembly:ASM223467v1:17:15543647:15547570:1 gene:ENSORLG00000023375.1 transcript:ENSORLT00000043369.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 6.691e-8
Identity = 21/57 (36.84%), Postives = 29/57 (50.88%), Query Frame = 2

Query:  128 HRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRREPNQSRL 298
             R E       C +CGR GH  + C    NK C NCG++GHFA+ C  +     +R+
Sbjct:  206 QRQEGGEHRIKCFRCGRSGHLSRECTITKNKKCNNCGKEGHFAKMCKTKMTHKVNRV 262

[Close]

BLAST of SMED30017899 vs. Ensembl Medaka
Match: cnbpa (CCHC-type zinc finger nucleic acid binding protein [Source:NCBI gene;Acc:101173222])

HSP 1 Score: 46.9802 bits (110), Expect = 2.973e-7
Identity = 26/66 (39.39%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +ANE KC +C   GH  + C +   C +CG  GH    C + +   CYNCG+ GH A++C I
Sbjct:   76 RDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVHCSKASETNCYNCGKAGHLAKECTI 140

[Close]

BLAST of SMED30017899 vs. Ensembl Medaka
Match: cnbpa (CCHC-type zinc finger nucleic acid binding protein [Source:NCBI gene;Acc:101173222])

HSP 1 Score: 47.3654 bits (111), Expect = 3.415e-7
Identity = 26/66 (39.39%), Postives = 36/66 (54.55%), Query Frame = 2

Query:   77 RSNPNANE-KCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNI 271
            R   +ANE KC +C   GH  + C +   C +CG  GH    C + +   CYNCG+ GH A++C I
Sbjct:   98 RDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVHCSKASETNCYNCGKAGHLAKECTI 162

[Close]

BLAST of SMED30017899 vs. Planmine SMEST
Match: SMESG000074160.1 (SMESG000074160.1)

HSP 1 Score: 242.662 bits (618), Expect = 2.756e-75
Identity = 121/128 (94.53%), Postives = 124/128 (96.88%), Query Frame = 2

Query:    2 QMFKPPATRNPEDRGNNQVSYNNNFRSNPNANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRREPNQSRLNNSMGNPQINVMQEIDMLRETLQRMPMEL 385
            QMFKPP TRNPED+GNNQVSYNNNFR+NPNANEKCSTCQRIGHRSENCFRNY CQ CG+RGHTEKICRQPNNKACYNCGQQGH ARQCNIRREPNQSRLNNSMGNPQINVMQEIDMLRETLQRMPMEL
Sbjct:  396 QMFKPPVTRNPEDKGNNQVSYNNNFRNNPNANEKCSTCQRIGHRSENCFRNYNCQICGKRGHTEKICRQPNNKACYNCGQQGHLARQCNIRREPNQSRLNNSMGNPQINVMQEIDMLRETLQRMPMEL 523

[Close]

BLAST of SMED30017899 vs. Planmine SMEST
Match: SMESG000019863.1 (SMESG000019863.1)

HSP 1 Score: 232.261 bits (591), Expect = 1.348e-71
Identity = 109/129 (84.50%), Postives = 118/129 (91.47%), Query Frame = 2

Query:    2 QMFKPPATRNPEDRGNNQVSYNNNFRSNPNANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRREPNQSRLNNS-MGNPQINVMQEIDMLRETLQRMPMEL 385
            QMFKPP TRNPED+GNNQVSYNNNFR+ PNANE+CSTCQ++GHRSENCFRNY CQKCGRRGHTE+ICRQ +NKACYNCGQQGH AR+CNIR E NQ R NN+ M NPQINVMQEIDMLRETLQRMPMEL
Sbjct:  234 QMFKPPTTRNPEDKGNNQVSYNNNFRNKPNANEQCSTCQKMGHRSENCFRNYNCQKCGRRGHTERICRQLDNKACYNCGQQGHIARRCNIRGEANQPRPNNNPMRNPQINVMQEIDMLRETLQRMPMEL 362

[Close]

BLAST of SMED30017899 vs. Planmine SMEST
Match: SMESG000019863.1 (SMESG000019863.1)

HSP 1 Score: 232.261 bits (591), Expect = 1.442e-71
Identity = 109/129 (84.50%), Postives = 118/129 (91.47%), Query Frame = 2

Query:    2 QMFKPPATRNPEDRGNNQVSYNNNFRSNPNANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRREPNQSRLNNS-MGNPQINVMQEIDMLRETLQRMPMEL 385
            QMFKPP TRNPED+GNNQVSYNNNFR+ PNANE+CSTCQ++GHRSENCFRNY CQKCGRRGHTE+ICRQ +NKACYNCGQQGH AR+CNIR E NQ R NN+ M NPQINVMQEIDMLRETLQRMPMEL
Sbjct:  234 QMFKPPTTRNPEDKGNNQVSYNNNFRNKPNANEQCSTCQKMGHRSENCFRNYNCQKCGRRGHTERICRQLDNKACYNCGQQGHIARRCNIRGEANQPRPNNNPMRNPQINVMQEIDMLRETLQRMPMEL 362

[Close]

BLAST of SMED30017899 vs. Planmine SMEST
Match: SMESG000019863.1 (SMESG000019863.1)

HSP 1 Score: 232.261 bits (591), Expect = 1.528e-71
Identity = 109/129 (84.50%), Postives = 118/129 (91.47%), Query Frame = 2

Query:    2 QMFKPPATRNPEDRGNNQVSYNNNFRSNPNANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRREPNQSRLNNS-MGNPQINVMQEIDMLRETLQRMPMEL 385
            QMFKPP TRNPED+GNNQVSYNNNFR+ PNANE+CSTCQ++GHRSENCFRNY CQKCGRRGHTE+ICRQ +NKACYNCGQQGH AR+CNIR E NQ R NN+ M NPQINVMQEIDMLRETLQRMPMEL
Sbjct:  234 QMFKPPTTRNPEDKGNNQVSYNNNFRNKPNANEQCSTCQKMGHRSENCFRNYNCQKCGRRGHTERICRQLDNKACYNCGQQGHIARRCNIRGEANQPRPNNNPMRNPQINVMQEIDMLRETLQRMPMEL 362

[Close]

BLAST of SMED30017899 vs. Planmine SMEST
Match: SMESG000059476.1 (SMESG000059476.1)

HSP 1 Score: 218.394 bits (555), Expect = 8.902e-67
Identity = 99/128 (77.34%), Postives = 112/128 (87.50%), Query Frame = 2

Query:    2 QMFKPPATRNPEDRGNNQVSYNNNFRSNPNANEKCSTCQRIGHRSENCFRNYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRREPNQSRLNNSMGNPQINVMQEIDMLRETLQRMPMEL 385
            QMFKPP TRNPE+ GNNQVSYNNNFR+NP ANE C+TCQRIGHR+E+C+RNY CQKCGRRGH EK+C Q +N+ACYNC ++GH AR CN+RR PNQ R NNS GNPQINVMQEID+LRET QRMPMEL
Sbjct:  309 QMFKPPPTRNPENGGNNQVSYNNNFRNNPKANEICTTCQRIGHRAEDCYRNYNCQKCGRRGHIEKVCGQLDNRACYNCNRKGHLARNCNMRRGPNQQRTNNSTGNPQINVMQEIDLLRETSQRMPMEL 436

The following BLAST results are available for this feature:

BLAST of SMED30017899 vs. Ensembl Human
BLAST of SMED30017899 vs. Ensembl Celegans
BLAST of SMED30017899 vs. Ensembl Fly
BLAST of SMED30017899 vs. Ensembl Zebrafish
BLAST of SMED30017899 vs. Ensembl Xenopus
BLAST of SMED30017899 vs. Ensembl Mouse
BLAST of SMED30017899 vs. UniProt/SwissProt
BLAST of SMED30017899 vs. TrEMBL
BLAST of SMED30017899 vs. Ensembl Cavefish
BLAST of SMED30017899 vs. Ensembl Sea Lamprey
BLAST of SMED30017899 vs. Ensembl Yeast
BLAST of SMED30017899 vs. Ensembl Nematostella
BLAST of SMED30017899 vs. Ensembl Medaka
BLAST of SMED30017899 vs. Planmine SMEST

BLAST of SMED30017899 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CNBP	6.192e-7	37.88	CCHC-type zinc finger nucleic acid binding protein...	[more]
CNBP	6.714e-7	37.88	CCHC-type zinc finger nucleic acid binding protein...	[more]
CNBP	6.831e-7	37.88	CCHC-type zinc finger nucleic acid binding protein...	[more]
CNBP	7.286e-7	37.88	CCHC-type zinc finger nucleic acid binding protein...	[more]
CNBP	7.866e-7	37.88	CCHC-type zinc finger nucleic acid binding protein...	[more]

BLAST of SMED30017899 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
glh-4	5.426e-9	36.62	ATP-dependent RNA helicase glh-4 [Source:UniProtK...	[more]
glh-4	5.426e-9	36.62	ATP-dependent RNA helicase glh-4 [Source:UniProtK...	[more]

BLAST of SMED30017899 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
CNBP	9.097e-6	36.62	gene:FBgn0034802 transcript:FBtr0303464	[more]
CNBP	9.097e-6	36.62	gene:FBgn0034802 transcript:FBtr0071994	[more]

BLAST of SMED30017899 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cnbpb	1.994e-7	37.97	CCHC-type zinc finger, nucleic acid binding protei...	[more]
cnbpb	1.994e-7	37.97	CCHC-type zinc finger, nucleic acid binding protei...	[more]
cnbpa	6.667e-7	40.91	CCHC-type zinc finger, nucleic acid binding protei...	[more]
cnbpa	6.667e-7	40.91	CCHC-type zinc finger, nucleic acid binding protei...	[more]
cnbpa	6.667e-7	40.91	CCHC-type zinc finger, nucleic acid binding protei...	[more]

BLAST of SMED30017899 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30017899 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Zcchc13	1.673e-8	36.67	zinc finger, CCHC domain containing 13 [Source:MGI...	[more]
Cnbp	4.609e-7	37.88	cellular nucleic acid binding protein [Source:MGI ...	[more]
Cnbp	4.609e-7	37.88	cellular nucleic acid binding protein [Source:MGI ...	[more]
Cnbp	5.002e-7	37.88	cellular nucleic acid binding protein [Source:MGI ...	[more]
Cnbp	5.400e-7	37.88	cellular nucleic acid binding protein [Source:MGI ...	[more]

BLAST of SMED30017899 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|O65639\|CSP1_ARATH	4.126e-8	35.06	Cold shock protein 1 OS=Arabidopsis thaliana OX=37...	[more]
sp\|O76743\|GLH4_CAEEL	6.901e-8	36.62	ATP-dependent RNA helicase glh-4 OS=Caenorhabditis...	[more]
sp\|Q89928\|POL_HV2EH	7.778e-8	44.19	Gag-Pol polyprotein OS=Human immunodeficiency viru...	[more]
sp\|Q9Q720\|POL_HV1V9	1.700e-7	41.86	Gag-Pol polyprotein OS=Human immunodeficiency viru...	[more]
sp\|Q76634\|POL_HV2UC	1.786e-7	47.50	Gag-Pol polyprotein OS=Human immunodeficiency viru...	[more]

BLAST of SMED30017899 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A261Y734	4.445e-10	50.91	Uncharacterized protein OS=Bifiguratus adelaidae O...	[more]
A0A1R0H6H2	2.792e-9	42.65	DNA-binding protein HEXBP OS=Smittium mucronatum O...	[more]
A0A396HR19	8.089e-9	40.63	Putative nucleotidyltransferase, Ribonuclease H OS...	[more]
A0A146I6E9	1.445e-8	39.66	Uncharacterized protein OS=Mycena chlorophos OX=65...	[more]
A0A146I6C1	1.446e-8	39.66	Uncharacterized protein OS=Mycena chlorophos OX=65...	[more]

BLAST of SMED30017899 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ENSAMXT00000005562.2	1.119e-7	37.97	cellular nucleic acid-binding protein [Source:NCBI...	[more]
cnbpa	2.523e-7	40.91	cellular nucleic acid-binding protein-like [Source...	[more]
ENSAMXT00000033510.1	3.304e-6	28.87	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of SMED30017899 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30017899 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
GIS2	1.260e-8	35.38	Translational activator for mRNAs with internal ri...	[more]

BLAST of SMED30017899 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30017899 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ENSORLT00000043369.1	6.691e-8	36.84	pep primary_assembly:ASM223467v1:17:15543647:15547...	[more]
cnbpa	2.973e-7	39.39	CCHC-type zinc finger nucleic acid binding protein...	[more]
cnbpa	3.415e-7	39.39	CCHC-type zinc finger nucleic acid binding protein...	[more]

BLAST of SMED30017899 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000074160.1	2.756e-75	94.53	SMESG000074160.1	[more]
SMESG000019863.1	1.348e-71	84.50	SMESG000019863.1	[more]
SMESG000019863.1	1.442e-71	84.50	SMESG000019863.1	[more]
SMESG000019863.1	1.528e-71	84.50	SMESG000019863.1	[more]
SMESG000059476.1	8.902e-67	77.34	SMESG000059476.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30017899 ID=SMED30017899|Name=SMED30017899|organism=Schmidtea mediterranea sexual|type=transcript|length=386bp
GCAGATGTTCAAGCCACCAGCAACGAGAAATCCGGAGGATAGAGGTAATA
ACCAAGTAAGTTATAATAATAATTTCAGGAGCAATCCAAACGCAAACGAA
AAATGCTCCACATGCCAAAGAATAGGACATCGGTCAGAGAATTGTTTCCG
AAATTACACTTGTCAAAAATGTGGTAGACGAGGGCATACTGAGAAAATAT
GTAGACAACCTAATAACAAAGCATGTTACAATTGTGGACAACAAGGACAT
TTCGCAAGGCAATGTAACATAAGAAGAGAACCAAACCAGTCAAGATTAAA
CAACTCTATGGGAAATCCACAAATTAACGTGATGCAAGAGATAGACATGC
TACGAGAAACACTACAACGAATGCCCATGGAATTAA

protein sequence of SMED30017899-orf-1

>SMED30017899-orf-1 ID=SMED30017899-orf-1|Name=SMED30017899-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=128bp
QMFKPPATRNPEDRGNNQVSYNNNFRSNPNANEKCSTCQRIGHRSENCFR
NYTCQKCGRRGHTEKICRQPNNKACYNCGQQGHFARQCNIRREPNQSRLN
NSMGNPQINVMQEIDMLRETLQRMPMEL

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000070	intestinal phagocyte
PLANA:0000074	oocyte
PLANA:0000231	vitelline gland

Vocabulary: **INTERPRO**
Term	Definition
IPR001878	Znf_CCHC
IPR025836	Zn_knuckle_CX2CX4HX4C

Vocabulary: **molecular function**
Term	Definition
GO:0008270	zinc ion binding
GO:0003676	nucleic acid binding

Main menu

SMED30017899

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 18

Owlery

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