tRNA pseudouridine synthase A

Overview
NametRNA pseudouridine synthase A
Smed IDSMED30017871
Length (bp)1449
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tRNA pseudouridine synthase A (SMED30017871) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA pseudouridine synthase A (SMED30017871) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA pseudouridine synthase A (SMED30017871) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA pseudouridine synthase A (SMED30017871) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017871

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30017871h1SMcG0007866 SmedASXL_011302SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30017871h1SMcG0007866 dd_Smed_v4_7557_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30017871h1SMcG0007866 dd_Smed_v4_7557_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30017871h1SMcG0007866 dd_Smed_v4_7557_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30017871h1SMcG0007866 dd_Smed_v4_7557_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30017871h1SMcG0007866 dd_Smed_v4_7557_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Human
Match: PUS1 (pseudouridine synthase 1 [Source:HGNC Symbol;Acc:HGNC:15508])

HSP 1 Score: 301.982 bits (772), Expect = 3.985e-98
Identity = 142/328 (43.29%), Postives = 216/328 (65.85%), Query Frame = 2 
Query:  389 ESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            E ++ K  KR   ++M YSG GY GMQRN G   FKTIE D++ A++ +  I     +   K+ FQR ++TDK VSA GQV S+K+   +D+++ +NS LP  IRIL + + T  F++K     RTY YL P+FAF+ K+  + +E    +R +  T  +VNR+L+ YKGTHNFHNFTS++ P D S  RY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+ +Y  I   E +E SM  WL TL +H++
Sbjct:   47 EERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDE---TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 371
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Human
Match: PUS1 (pseudouridine synthase 1 [Source:HGNC Symbol;Acc:HGNC:15508])

HSP 1 Score: 301.597 bits (771), Expect = 7.976e-98
Identity = 142/328 (43.29%), Postives = 216/328 (65.85%), Query Frame = 2 
Query:  389 ESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            E ++ K  KR   ++M YSG GY GMQRN G   FKTIE D++ A++ +  I     +   K+ FQR ++TDK VSA GQV S+K+   +D+++ +NS LP  IRIL + + T  F++K     RTY YL P+FAF+ K+  + +E    +R +  T  +VNR+L+ YKGTHNFHNFTS++ P D S  RY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+ +Y  I   E +E SM  WL TL +H++
Sbjct:   47 EERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDE---TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 371
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Human
Match: PUS1 (pseudouridine synthase 1 [Source:HGNC Symbol;Acc:HGNC:15508])

HSP 1 Score: 301.597 bits (771), Expect = 1.840e-97
Identity = 142/328 (43.29%), Postives = 216/328 (65.85%), Query Frame = 2 
Query:  389 ESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            E ++ K  KR   ++M YSG GY GMQRN G   FKTIE D++ A++ +  I     +   K+ FQR ++TDK VSA GQV S+K+   +D+++ +NS LP  IRIL + + T  F++K     RTY YL P+FAF+ K+  + +E    +R +  T  +VNR+L+ YKGTHNFHNFTS++ P D S  RY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+ +Y  I   E +E SM  WL TL +H++
Sbjct:   75 EERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDE---TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 399
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Human
Match: PUS1 (pseudouridine synthase 1 [Source:HGNC Symbol;Acc:HGNC:15508])

HSP 1 Score: 277.33 bits (708), Expect = 1.010e-88
Identity = 130/302 (43.05%), Postives = 200/302 (66.23%), Query Frame = 2 
Query:  467 QRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            QRN G   FKTIE D++ A++ +  I     +   K+ FQR ++TDK VSA GQV S+K+   +D+++ +NS LP  IRIL + + T  F++K     RTY YL P+FAF+ K+  + +E    +R +  T  +VNR+L+ YKGTHNFHNFTS++ P D S  RY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+ +Y  I   E +E SM  WL TL +H++
Sbjct:   48 QRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDE---TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 346
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Human
Match: PUS1 (pseudouridine synthase 1 [Source:HGNC Symbol;Acc:HGNC:15508])

HSP 1 Score: 90.8929 bits (224), Expect = 5.892e-21
Identity = 48/107 (44.86%), Postives = 68/107 (63.55%), Query Frame = 2 
Query:  389 ESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRIL 700
            E ++ K  KR   ++M YSG GY GMQRN G   FKTIE D++ A++ +  I     +   K+ FQR ++TDK VSA GQV S+K+   +D+++ +NS LP  IRIL
Sbjct:   47 EERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRIL 153
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Celegans
Match: pus-1 (tRNA pseudouridine synthase [Source:UniProtKB/TrEMBL;Acc:B1V8I7])

HSP 1 Score: 213.001 bits (541), Expect = 7.164e-64
Identity = 119/337 (35.31%), Postives = 194/337 (57.57%), Query Frame = 2 
Query:  389 ESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFN-----DVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFS-PKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVF------QENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPI-NW-LVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            E KKP+  +   A+++ Y G  Y+GMQ  +   TIE  +L+AM  A  I   + ++P    FQRA++TD+ VSA  Q+C ++L R +     +    LN+ LP  IR+  + +TT  FH +     RTY Y  PSF F+ P E++     +FR +  T  +VN +LS Y GTHNF N+T+KR   D S  RY+++  C+  F      ++  +E+V I++ GQSF+LHQIRKM+G+ + V+R    ++  ++ F+ Q++DIP APGLGL+L++ +Y+SY+ K +     +P+ +W  + + +IE  + + +   + + E     M  WL  L  H +
Sbjct:   24 EPKKPRVKQAKYAMLLGYQGKKYYGMQLQKDLPTIESKLLEAMETAGWITTAQKEKPFDFFFQRAARTDRAVSAARQMCGMQLPRDDAKYQAEGAGILNNILPADIRVFGMRRTTNFFHPQKQCDHRTYSYTCPSFVFAKPTELTNS---SFRLSPETLAEVNSILSIYLGTHNFFNYTAKRAYDDMSSNRYIISFECKEPFLFRDDFRKEDVEFVQIVIKGQSFVLHQIRKMVGMVITVIRELHLKSSIQRSFEGQRMDIPMAPGLGLLLERTHYDSYDRKNS--KSHEPLTDWGEIIEKEIERTKFELITKDMIETELLTQGMLKWLADLVHHDF 355
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Celegans
Match: tag-124 (Probable tRNA pseudouridine synthase tag-124 [Source:UniProtKB/Swiss-Prot;Acc:Q09524])

HSP 1 Score: 57.7658 bits (138), Expect = 7.373e-9
Identity = 77/329 (23.40%), Postives = 127/329 (38.60%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGYFGM-QRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSR---------FND------------------VVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNF-----TSKRLPTDSSCRRYLMNLTCESVFQENGI-EYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILD------KVNYES---------YNNKYAVEGGRKPINWLVYQSQ 1252
            +R  AI   Y G  + G+ Q+     T+E  ++QA++   LI++       K  F R  +TDK VSA  Q  ++ +           ++D                   V  LN  LP  IR+       ++F+A+     RTY+Y   SFA +               ++  +K+ +      G H+F NF       KRL      + Y + +   S   EN +   V + V+G  F+ H IR ++ +   + R     ++  ++  L+K   P  P   L  D         Y+S         Y  K  V G +K   W  YQ++
Sbjct:   29 RRKIAIQFFYLGWEHDGLVQQPHTQNTVENHIMQALIKTHLIEDW-----TKCDFSRCGRTDKGVSAFKQTAAMVVRSLCPGDSGVFWSDSTQEHQKVDYKASGEELPYVKMLNGVLPKTIRVFAWAPVAQTFNARFDCNRRTYKY---SFAKA---------------DLNLEKMRQGAELLVGEHDFSNFCQIDMNEKRLLQSYVRKVYEVKVEQVSTHPENDMYSMVELTVSGSGFLWHMIRYIVTILQEIGRENEQPSLISQLLDLKKY--PSRPQYTLASDTPLCLFDCGYKSEDVEWKVHDYTLKSTVTGLQK--TWATYQAR 330
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Fly
Match: Pus1 (gene:FBgn0038811 transcript:FBtr0083944)

HSP 1 Score: 288.115 bits (736), Expect = 1.002e-92
Identity = 143/320 (44.69%), Postives = 205/320 (64.06%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELIN-FRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQE-NGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFR 1366
            ++  AI++ Y G  Y+GMQRN G +TIE ++ +AML  + I  + F+Q     FQRA++TDK VSA  QVCSVKL    D+ +  N+ LP QIR+  V + TK F+AK     RTY Y  P+ AF+P E   +++ + FR +     KV   L  Y+GT NFHNFTSK+   D S +R++M+ T    F+    IE+V++ V GQSFMLHQIRKM+GLA+A+VRG       E+    ++LD+P APGLGL+LD V+YE YN++Y  +G   P+ W   ++Q+++F E+ ++S I+K E E+ +M DW+ TL  HSYD R
Sbjct:   69 RKKSAILLSYCGANYYGMQRNPGMQTIEEELFKAMLKHKWITEDSFEQIQISCFQRAARTDKGVSAARQVCSVKLPEELDL-EAFNADLPQQIRLFGVERVTKGFNAKDQCNARTYTYTLPTVAFAPFEEKVDDVHDTFRISPELLQKVKETLKLYEGTKNFHNFTSKKSFLDPSSKRFIMSFTSSEPFRSPQDIEFVTLKVKGQSFMLHQIRKMVGLAIAIVRGNTTAATLERALTEERLDLPMAPGLGLVLDTVHYERYNDRYGKDGIHNPLTWQAQEAQVQEFIEREIFSQIYKTEAEQRNMLDWIGTLHYHSYDTR 387
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Fly
Match: Pus1 (gene:FBgn0038811 transcript:FBtr0302323)

HSP 1 Score: 287.73 bits (735), Expect = 3.468e-92
Identity = 143/320 (44.69%), Postives = 205/320 (64.06%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELIN-FRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQE-NGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFR 1366
            ++  AI++ Y G  Y+GMQRN G +TIE ++ +AML  + I  + F+Q     FQRA++TDK VSA  QVCSVKL    D+ +  N+ LP QIR+  V + TK F+AK     RTY Y  P+ AF+P E   +++ + FR +     KV   L  Y+GT NFHNFTSK+   D S +R++M+ T    F+    IE+V++ V GQSFMLHQIRKM+GLA+A+VRG       E+    ++LD+P APGLGL+LD V+YE YN++Y  +G   P+ W   ++Q+++F E+ ++S I+K E E+ +M DW+ TL  HSYD R
Sbjct:  101 RKKSAILLSYCGANYYGMQRNPGMQTIEEELFKAMLKHKWITEDSFEQIQISCFQRAARTDKGVSAARQVCSVKLPEELDL-EAFNADLPQQIRLFGVERVTKGFNAKDQCNARTYTYTLPTVAFAPFEEKVDDVHDTFRISPELLQKVKETLKLYEGTKNFHNFTSKKSFLDPSSKRFIMSFTSSEPFRSPQDIEFVTLKVKGQSFMLHQIRKMVGLAIAIVRGNTTAATLERALTEERLDLPMAPGLGLVLDTVHYERYNDRYGKDGIHNPLTWQAQEAQVQEFIEREIFSQIYKTEAEQRNMLDWIGTLHYHSYDTR 419
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Match: pus1 (pseudouridine synthase 1 [Source:ZFIN;Acc:ZDB-GENE-041212-26])

HSP 1 Score: 293.893 bits (751), Expect = 7.241e-95
Identity = 140/334 (41.92%), Postives = 217/334 (64.97%), Query Frame = 2 
Query:  368 EDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRN---EGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYD 1360
            ED +L ++ ++ K  K+  A++M YSG GY+GMQRN     FKTIE +++ A++ A  I     ++  K+ FQR+++TDK VSA+GQV S+K+    ++++ +N+ LP QIR+L   + T  F++K     RTY Y+ P+ AFSPK+ ++E+  +FR    T   VNR+   YKGTHNFHNFTS++   D S  RY+ +++C   F    +E+  I V GQSFM+HQIRKMIGL +AVV+GY DE + EK +  +K+ +PKAPGLGL+L++V+++ YN ++  +G  + + W   +  I DF+EK +Y  I   E  E SM +W+ +L +H ++
Sbjct:   60 EDKKLKTDQEERKYPKKKVALLMAYSGKGYYGMQRNARNSQFKTIEDELVTALVGARCIPEGHAEEMKKMSFQRSARTDKGVSAVGQVVSLKVWMIENILEKINAHLPPQIRVLGCKRVTGGFNSKNNCYARTYSYMLPTVAFSPKDYNQED-TSFRLTSDTLQTVNRLFGLYKGTHNFHNFTSQKGHRDPSAMRYITHMSCGEPFIRKDVEFAVITVRGQSFMMHQIRKMIGLVIAVVKGYVDEGVIEKSWGEEKVGVPKAPGLGLVLERVHFDGYNKRFGGDGIHETLEWTEQEEAIADFKEKHIYPTIVDTELNEKSMVNWMTSLCIHDFE 392
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Match: pus1 (pseudouridine synthase 1 [Source:ZFIN;Acc:ZDB-GENE-041212-26])

HSP 1 Score: 249.21 bits (635), Expect = 2.528e-79
Identity = 113/262 (43.13%), Postives = 173/262 (66.03%), Query Frame = 2 
Query:  575 QRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYD 1360
            QR+++TDK VSA+GQV S+K+    ++++ +N+ LP QIR+L   + T  F++K     RTY Y+ P+ AFSPK+ ++E+  +FR    T   VNR+   YKGTHNFHNFTS++   D S  RY+ +++C   F    +E+  I V GQSFM+HQIRKMIGL +AVV+GY DE + EK +  +K+ +PKAPGLGL+L++V+++ YN ++  +G  + + W   +  I DF+EK +Y  I   E  E SM +W+ +L +H ++
Sbjct:    2 QRSARTDKGVSAVGQVVSLKVWMIENILEKINAHLPPQIRVLGCKRVTGGFNSKNNCYARTYSYMLPTVAFSPKDYNQED-TSFRLTSDTLQTVNRLFGLYKGTHNFHNFTSQKGHRDPSAMRYITHMSCGEPFIRKDVEFAVITVRGQSFMMHQIRKMIGLVIAVVKGYVDEGVIEKSWGEEKVGVPKAPGLGLVLERVHFDGYNKRFGGDGIHETLEWTEQEEAIADFKEKHIYPTIVDTELNEKSMVNWMTSLCIHDFE 262
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Match: pusl1 (pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB-GENE-101110-1])

HSP 1 Score: 56.225 bits (134), Expect = 2.950e-8
Identity = 66/279 (23.66%), Postives = 120/279 (43.01%), Query Frame = 2 
Query:  428 IMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFND--------VVDTLNSKLPLQ-IRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE-----ISEEELI-NFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSS--CRRYLMNLT----CESVFQ---ENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQ-KLDIPK---APGLGLILDKVNY 1180
            I   Y+G  Y G+ +    + ++G V   + +A      + K   ++    +S+TD  V AL     V + R  D        +++ LN +L  + IRI   ++    FHA+   + RTY Y     A   +      ++E++L    R   +  D +    +   GTH+F  F  + L +D+   C    ++L      ES  Q   +  I++  +    +SF+  Q+R+M G+ +A+ +G    +  + + + +  L  P    AP  GL L  V Y
Sbjct:   10 IFFQYTGSKYSGVMKAPAHQAVQG-VGNHLENAV----RKLKPVNEVCVSISSRTDTGVHALCNSAHVDIQRRADKPPLSEQSLLEALNFQLKEEPIRITRAYRVHSDFHARYQAQSRTYVY---RLALGCQHHTLMPLTEKDLCWALRDTRLNIDAMREASALLLGTHDFSTF--RALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQRDIQFWELTFKSRSFLYRQVRRMTGVLVAIGQGRLSVSKIQDLLEARDSLAFPNNLTAPAHGLFLTNVQY 278
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Match: pusl1 (pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB-GENE-101110-1])

HSP 1 Score: 55.8398 bits (133), Expect = 3.012e-8
Identity = 66/279 (23.66%), Postives = 120/279 (43.01%), Query Frame = 2 
Query:  428 IMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFND--------VVDTLNSKLPLQ-IRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE-----ISEEELI-NFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSS--CRRYLMNLT----CESVFQ---ENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQ-KLDIPK---APGLGLILDKVNY 1180
            I   Y+G  Y G+ +    + ++G V   + +A      + K   ++    +S+TD  V AL     V + R  D        +++ LN +L  + IRI   ++    FHA+   + RTY Y     A   +      ++E++L    R   +  D +    +   GTH+F  F  + L +D+   C    ++L      ES  Q   +  I++  +    +SF+  Q+R+M G+ +A+ +G    +  + + + +  L  P    AP  GL L  V Y
Sbjct:   10 IFFQYTGSKYSGVMKAPAHQAVQG-VGNHLENAV----RKLKPVNEVCVSISSRTDTGVHALCNSAHVDIQRRADKPPLSEQSLLEALNFQLKEEPIRITRAYRVHSDFHARYQAQSRTYVY---RLALGCQHHTLMPLTEKDLCWALRDTRLNIDAMREASALLLGTHDFSTF--RALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQRDIQFWELTFKSRSFLYRQVRRMTGVLVAIGQGRLSVSKIQDLLEARDSLAFPNNLTAPAHGLFLTNVQY 278
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Match: PUS1 (pseudouridylate synthase 1 [Source:NCBI gene;Acc:100380023])

HSP 1 Score: 306.99 bits (785), Expect = 6.341e-100
Identity = 148/322 (45.96%), Postives = 215/322 (66.77%), Query Frame = 2 
Query:  404 KQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYD 1360
            K  KR   ++M YSG GY GMQRN G   FKTIE +++QA++ +  I +   +   K+ FQR ++TDK VSA GQV S+K+   ++VV+ +N  LP  IRIL + + T  F++K     RTY Y  P+FAFS K+  E +  NFR ++ T ++VN +L+ YKGT NFHNFTS++ P D S +RY+M + CE  FQ+ G+E   I V GQSFM+HQIRKMIGL +AVV+G+   +I E+ +  +K+DIPKAPGLGL+L++V++E YN ++  +G  + +NW   + +IE F+++ +Y  I + E +E SM  WL TL +H YD
Sbjct:   51 KHPKRKVVLLMAYSGKGYHGMQRNVGSSQFKTIEDELVQALVLSGCIPDNHAEDMKKMSFQRCARTDKGVSAAGQVVSLKIWLIDNVVEKINENLPSHIRILGLKRVTGRFNSKNSCDARTYSYTLPTFAFSHKD-KEAQDENFRLSQETLNRVNELLALYKGTRNFHNFTSQKGPRDPSAKRYIMEMHCEPPFQQGGLELAVIKVKGQSFMMHQIRKMIGLVIAVVKGFAPTSIIERSWGEEKVDIPKAPGLGLVLERVHFEKYNRRFGNDGLHESLNWTEEEEKIESFKKEHIYPTIIQTEIDEKSMLAWLATLPIHDYD 371
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Match: ENSXETT00000026732.1 (tRNA pseudouridine synthase A, mitochondrial-like [Source:NCBI gene;Acc:100490752])

HSP 1 Score: 247.669 bits (631), Expect = 8.457e-77
Identity = 133/354 (37.57%), Postives = 208/354 (58.76%), Query Frame = 2 
Query:  308 MPEIKNDILEESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRN---EGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKL-SRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            M + K D+  E        +  +E+L  +   +  KR  AIM+ Y G GY+GMQ +     F TIE  ++ A++ A  I    + Q  ++KFQR ++TDK VSAL Q+ SV+L    ++ V+ +NS LP +I I+D+ + TK F  K M   R+Y Y+ P+FA S    S  +  NFR     F  +N +LS+YKGTHNFHNFTS++   D S  R++++++C   F  +G E+  I + GQSFML+QIRKM+GL +A+ +G        +  Q +K++IP APGLGL+L++V+++ YN +Y  +G  +PI+W      +  F E+ +   I + E E  SM  W++ L+ H++
Sbjct:   49 MAKNKEDVPHEELDPAGTINASVEVLENNSNIQIPKRKFAIMLAYCGRGYYGMQMDLTRPNFPTIESALISALIQARCIPEMFYMQMKQLKFQRCARTDKGVSALSQLVSVRLLPSCSNPVEKINSHLPPEILIIDMKRVTKGFCPKKMCDKRSYSYMVPTFALSCCAPSVPD-SNFRMPREDFHNINNLLSFYKGTHNFHNFTSRKAFEDPSSFRHMLDVSCSEPFVFHGTEFAQIHITGQSFMLYQIRKMVGLIIAIAQGIVPADFLPQCMQREKINIPPAPGLGLVLERVHFDWYNKRYGGDGFHQPISWEKSMPTVSVFWEERILPDILEGELENLSMSYWIEKLNRHNF 401
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Match: prkd3 (protein kinase D3 [Source:Xenbase;Acc:XB-GENE-6036037])

HSP 1 Score: 78.9518 bits (193), Expect = 4.262e-15
Identity = 73/264 (27.65%), Postives = 114/264 (43.18%), Query Frame = 2 
Query:  392 SKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFK-TIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFND------VVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDS-SCRRYLMNLTCESVFQENG----IEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAP 1147
            S  PKQ     A+ + Y G GY G    E  K T+E  V  A++   L+++ +        + R  +TD+ VSALGQV S+ L    D       V  LN  LP  IR+L       SF A+   + RTYRYL P                 + +    DK  R+L   +GTH+F N     +     +  R +++   E V +  G     +   + + G +F+ HQ+R ++G+   V +G     +  ++     LD+ K P
Sbjct:   57 SAHPKQ---HVALRLAYLGWGYQGFASQENTKNTVEETVFNALIKTRLVESRQTS-----NYHRCGRTDRGVSALGQVISLDLRCSGDGKPEIRYVHILNRVLPPDIRVLSWAAVPPSFSARFSCRSRTYRYLFPC---------------AQLDVEAMDKAARLL---EGTHDFRNLCKMDVANGVMNFIRTVLSARVEPVPEIPGDMGPFQLYRLQITGLAFLYHQVRCIMGVLFLVGQGKEKPEVITEL-----LDVAKNP 289
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Match: PUSL1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:100497905])

HSP 1 Score: 59.6918 bits (143), Expect = 2.766e-9
Identity = 68/292 (23.29%), Postives = 118/292 (40.41%), Query Frame = 2 
Query:  410 CKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFND--------VVDTLNSKL-PLQIRILDVFQTTKSFHAKGMVKCRTYRY-LTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGI----------EYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIP----KAPGLGLILDKVNY-ESYNNKYAVE 1210
            CK    +   Y G  Y G+      +++ G  +Q  L+   +  ++ +    +KF  +S+TD  V A+     V + R N         +V  LN  L P  IR+L   +   +FHA+     RTY Y +    + S   + EE L       +    ++       GTHNF  F S    +++  +  +  L    +   +GI          ++       +SF+  Q+R+M G  +AV +G       ++  + Q  +       AP  GL L+ V Y E+  N ++ E
Sbjct:    4 CKARYLVFFQYYGTKYSGVMETPVTQSVLG--VQNYLE---MAAQKLRPVGTVKFFISSRTDSGVHAICNSAHVDIQRANGKPPFTEDILVQALNHHLQPEPIRLLKAVRVCDNFHARHKALSRTYVYRVAAGCSHSELPVFEENLCWAVPGSLNVAAIHEASEMLLGTHNFSAFRSAN--SENVFKSPIKTLQQADITPSSGILTHHWQYRNLQFWDFTFRARSFLYKQVRRMTGALVAVGQGRLTPLQIKEFLENQDPNCTLGNFVAPPHGLFLNNVEYDETELNNFSAE 288
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Match: PUSL1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:100497905])

HSP 1 Score: 58.9214 bits (141), Expect = 4.860e-9
Identity = 66/285 (23.16%), Postives = 113/285 (39.65%), Query Frame = 2 
Query:  410 CKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAE---LIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFND--------VVDTLNSKL-PLQIRILDVFQTTKSFHAKGMVKCRTYRY-LTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGI----------EYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIP----KAPGLGLILDKVNYE 1183
            CK    +   Y G  Y G+        +E  V Q++L  +    +  ++ +    +KF  +S+TD  V A+     V + R N         +V  LN  L P  IR+L   +   +FHA+     RTY Y +    + S   + EE L       +    ++       GTHNF  F S    +++  +  +  L    +   +GI          ++       +SF+  Q+R+M G  +AV +G       ++  + Q  +       AP  GL L+ V Y+
Sbjct:    4 CKARYLVFFQYYGTKYSGV--------METPVTQSVLGVQNYLEMAAQKLRPVGTVKFFISSRTDSGVHAICNSAHVDIQRANGKPPFTEDILVQALNHHLQPEPIRLLKAVRVCDNFHARHKALSRTYVYRVAAGCSHSELPVFEENLCWAVPGSLNVAAIHEASEMLLGTHNFSAFRSAN--SENVFKSPIKTLQQADITPSSGILTHHWQYRNLQFWDFTFRARSFLYKQVRRMTGALVAVGQGRLTPLQIKEFLENQDPNCTLGNFVAPPHGLFLNNVEYD 278
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Mouse
Match: Pus1 (pseudouridine synthase 1 [Source:MGI Symbol;Acc:MGI:1929237])

HSP 1 Score: 303.138 bits (775), Expect = 1.180e-98
Identity = 143/331 (43.20%), Postives = 219/331 (66.16%), Query Frame = 2 
Query:  386 SESKKP--KQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
             E ++P  K  KR   ++M YSG GY GMQRN G   F+TIE D++ A++ A  I         K+ FQR ++TDK VSA GQV S+K+   +D++D +NS LP  IRIL + + T  F++K     RTY Y+ P+FAF+ K+  + +E   ++R +  T  +VNR+L+ YKGTHNFHNFTS++ P + S RRY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+++Y  I   E +E SM  WL TL +H++
Sbjct:   38 GEDEEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDE---SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 365
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Mouse
Match: Pus1 (pseudouridine synthase 1 [Source:MGI Symbol;Acc:MGI:1929237])

HSP 1 Score: 303.138 bits (775), Expect = 1.180e-98
Identity = 143/331 (43.20%), Postives = 219/331 (66.16%), Query Frame = 2 
Query:  386 SESKKP--KQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
             E ++P  K  KR   ++M YSG GY GMQRN G   F+TIE D++ A++ A  I         K+ FQR ++TDK VSA GQV S+K+   +D++D +NS LP  IRIL + + T  F++K     RTY Y+ P+FAF+ K+  + +E   ++R +  T  +VNR+L+ YKGTHNFHNFTS++ P + S RRY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+++Y  I   E +E SM  WL TL +H++
Sbjct:   38 GEDEEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDE---SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 365
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Mouse
Match: Pus1 (pseudouridine synthase 1 [Source:MGI Symbol;Acc:MGI:1929237])

HSP 1 Score: 303.523 bits (776), Expect = 2.529e-98
Identity = 145/338 (42.90%), Postives = 219/338 (64.79%), Query Frame = 2 
Query:  359 KASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            K  ED     E    K  KR   ++M YSG GY GMQRN G   F+TIE D++ A++ A  I         K+ FQR ++TDK VSA GQV S+K+   +D++D +NS LP  IRIL + + T  F++K     RTY Y+ P+FAF+ K+  + +E   ++R +  T  +VNR+L+ YKGTHNFHNFTS++ P + S RRY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+++Y  I   E +E SM  WL TL +H++
Sbjct:   66 KGGED-----EEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDE---SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Mouse
Match: Pus1 (pseudouridine synthase 1 [Source:MGI Symbol;Acc:MGI:1929237])

HSP 1 Score: 291.582 bits (745), Expect = 1.653e-93
Identity = 145/356 (40.73%), Postives = 219/356 (61.52%), Query Frame = 2 
Query:  359 KASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKT------------------VSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            K  ED     E    K  KR   ++M YSG GY GMQRN G   F+TIE D++ A++ A  I         K+ FQR ++TDK                   VSA GQV S+K+   +D++D +NS LP  IRIL + + T  F++K     RTY Y+ P+FAF+ K+  + +E   ++R +  T  +VNR+L+ YKGTHNFHNFTS++ P + S RRY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+++Y  I   E +E SM  WL TL +H++
Sbjct:   66 KGGED-----EEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKPLRTSACFCLYTGFLFHWGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDE---SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 413
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Mouse
Match: Pus1 (pseudouridine synthase 1 [Source:MGI Symbol;Acc:MGI:1929237])

HSP 1 Score: 254.603 bits (649), Expect = 2.035e-80
Identity = 127/335 (37.91%), Postives = 193/335 (57.61%), Query Frame = 2 
Query:  359 KASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            K  ED     E    K  KR   ++M YSG GY GMQ                                            VSA GQV S+K+   +D++D +NS LP  IRIL + + T  F++K     RTY Y+ P+FAF+ K+  + +E   ++R +  T  +VNR+L+ YKGTHNFHNFTS++ P + S RRY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+++Y  I   E +E SM  WL TL +H++
Sbjct:   36 KGGED-----EEPPRKLPKRKIVLLMAYSGKGYHGMQ-------------------------------------------GVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDE---SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 319
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|Q9WU56|TRUA_MOUSE (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 303.523 bits (776), Expect = 1.769e-97
Identity = 145/338 (42.90%), Postives = 219/338 (64.79%), Query Frame = 2 
Query:  359 KASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            K  ED     E    K  KR   ++M YSG GY GMQRN G   F+TIE D++ A++ A  I         K+ FQR ++TDK VSA GQV S+K+   +D++D +NS LP  IRIL + + T  F++K     RTY Y+ P+FAF+ K+  + +E   ++R +  T  +VNR+L+ YKGTHNFHNFTS++ P + S RRY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+++Y  I   E +E SM  WL TL +H++
Sbjct:   66 KGGED-----EEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDE---SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|Q4KM92|TRUA_RAT (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 303.138 bits (775), Expect = 1.929e-97
Identity = 145/338 (42.90%), Postives = 218/338 (64.50%), Query Frame = 2 
Query:  359 KASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            K  ED     E    K  KR   ++M YSG GY GMQRN G   F+TIE D++ A++ A  I         K+ FQR ++TDK VSA GQV S+K+   +D++D +NS LP  IRIL + + T  F++K     RTY Y+ P+FAF+ K+  + +E   ++R +  T  +VNR+L  YKGTHNFHNFTS++ P + S RRY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+++Y  I   E +E SM  WL TL +H++
Sbjct:   66 KGGED-----EEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKDRDVQDE---SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|Q9Y606|TRUA_HUMAN (tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3)

HSP 1 Score: 301.597 bits (771), Expect = 8.836e-97
Identity = 142/328 (43.29%), Postives = 216/328 (65.85%), Query Frame = 2 
Query:  389 ESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE--ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            E ++ K  KR   ++M YSG GY GMQRN G   FKTIE D++ A++ +  I     +   K+ FQR ++TDK VSA GQV S+K+   +D+++ +NS LP  IRIL + + T  F++K     RTY YL P+FAF+ K+  + +E    +R +  T  +VNR+L+ YKGTHNFHNFTS++ P D S  RY++ + CE  F   G+E+  I V GQSFM+HQIRKM+GL +A+V+GY  E++ E+ +  +K+D+PKAPGLGL+L++V++E YN ++  +G  +P++W   + ++  F+E+ +Y  I   E +E SM  WL TL +H++
Sbjct:   75 EERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDE---TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 399
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|Q6FV05|PUS1_CANGA (tRNA pseudouridine synthase 1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 1.308e-44
Identity = 116/381 (30.45%), Postives = 175/381 (45.93%), Query Frame = 2 
Query:  395 KKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLS-RFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCR-------TYRYLTPSFA--------------------------------------FSPKEIS---------------EEELIN---------------FRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVF--QENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIE 1303
            ++P+  KR  A+M+ Y G GY GMQ N    TIE  + +A +DA  I         K  F RA++TDK V A G + S+K+     +++D +N+ LP  IRI D+ +  K+F  + M   R       TY  + P                                         F+P+EI+               +EEL                 +R +E+  +K    ++ Y G HNFHNFT  +   D S  R++  +     F   +   E+VSI ++GQSFMLHQIRKMI +A  + R         + F  QK++IPKAP LGL+L+   +ESYN +   + G KPI++  YQ +++ F+ K +Y  I+  E
Sbjct:   80 REPRLPKRKVAVMIGYCGTGYHGMQYNPPNPTIEAALFKAFVDAGAISKANSNDLKKNNFMRAARTDKGVHAGGNLISLKMIIEDPEIMDKINANLPEGIRIWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGTILNNDIESSKTELPGVLDEDLESKEFWENFEAAAKKEFTPEEIAAIKDYSPPPREEFDDQEELYQKVKQWKLLENTHRRQYRISEMKLNKFRAAMNQYLGAHNFHNFTLGKEFKDPSAIRFMKEIKVSDPFVIGDAKAEWVSIKIHGQSFMLHQIRKMISMATLIARCGTPVERISQAFGPQKINIPKAPALGLLLEAPVFESYNKRLE-KFGYKPIDFSKYQEKVDAFKMKHIYDKIYAEE 459
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|O74451|YCG6_SCHPO (Putative tRNA pseudouridine synthase C16C4.06c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC16C4.06c PE=3 SV=2)

HSP 1 Score: 136.732 bits (343), Expect = 2.534e-34
Identity = 91/342 (26.61%), Postives = 164/342 (47.95%), Query Frame = 2 
Query:  386 SESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKL---SRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTP-SFAFSPKEISEEELI---------------------------------NFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQEN-GIEYVSIIVNGQSFMLHQIRKMIGLAMAVVR-GYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIF 1294
            S S KP+  KRM   ++ Y G G+ G+Q N   KTI+  + QA      +       P KI+   A++TDK V A+  V  +K+        VV+ +N  LP  IR+  + +T  SF    +   R Y Y  P S   +P+  + E  +                                  FR ++   D +    + ++GTH FH++T+++  +D+S RR+L+++  +++  +    ++V +I +GQSFM HQIRKM+G+ + + R G+  + +        ++ IP+AP   L+L++  ++++N K +     +P+ W   Q  I+ F   F+ + +F
Sbjct:   29 SISVKPR--KRMVYCVVSYRGTGFAGLQYNANVKTIQDTLFQAFARVGAVVQVNADSPKKIRMCSAARTDKGVHAIVNVLGLKVLDNQPLPHVVNLVNDILPPCIRVWKMARTFNSFSPHTVCDSRVYEYWLPVSSLLTPRPCTLEAYVIAKASEKAFPINETLSHLANLSKQDCVANSLSEPFRLSKPKLDFLKHACTMFRGTHRFHSYTTEKGFSDASSRRFLLDVRVDNLHIDKLNRQWVKLIFHGQSFMKHQIRKMVGILIHLTRTGWNAQVLLNTFNNSYRIRIPRAPAEFLLLNQPIFQAFNKKCS-RFDHEPVEWSCAQKGIDQFAHSFLRTPMF 367
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A0R3W683 (tRNA pseudouridine synthase OS=Taenia asiatica OX=60517 GN=TASK_LOCUS5686 PE=4 SV=2)

HSP 1 Score: 345.125 bits (884), Expect = 1.180e-111
Identity = 173/360 (48.06%), Postives = 238/360 (66.11%), Query Frame = 2 
Query:  350 LKRKASEDIELLSESKKPK---------QCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQP---------MKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPK-EISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGI-EYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD 1369
            +KR+   D E   ++K PK         +  R CAI+++YSG G+ GMQRNEGF+TIEG+++ A+ +A  I  E  K P         ++I FQRASKTDK VSA GQVC+ KL      VD +NS LP QIRILDVF+ TK F+ K     R Y+YL P+F+FSP   I+ E    +RF+  T  +VN +  YYKGTHNF NFTS+R P D SC+RY+M++ C S F  +G  E+  I V GQSFMLHQIRKMIGL +A+ +GY  E IFE VF+ +++D+P APGLGL+LD+V+Y  YN ++  +G  + I+W  Y+ QI++F+E +++ +I K E E++SM +W+  LS HSYD R+
Sbjct:    1 MKRQQEPDCEASYDAKAPKFDQVVSTGARGFRKCAILVVYSGAGFCGMQRNEGFRTIEGELMMALCNAGYILEEHMKNPSMFSNPVKIIQICFQRASKTDKNVSAAGQVCAAKLPIDTLSVDKINSHLPDQIRILDVFRVTKKFNPKTACNHRIYQYLLPTFSFSPGGSITLENCWKYRFSAETLTRVNELFRYYKGTHNFFNFTSRRTPDDKSCQRYIMSIECGSPFLLDGDREFALITVQGQSFMLHQIRKMIGLVIAIAKGYATEAIFENVFKTERMDVPMAPGLGLLLDQVDYTLYNKRFCADGSHQSIDWERYKRQIDEFKEAYIFQHIVKHEVEDNSMFEWMGNLSNHSYDRRE 360
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A068Y8Z5 (tRNA pseudouridine synthase A mitochondrial OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000889900 PE=4 SV=1)

HSP 1 Score: 338.576 bits (867), Expect = 6.482e-109
Identity = 161/320 (50.31%), Postives = 222/320 (69.38%), Query Frame = 2 
Query:  416 RMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFS-PKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENG-IEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD 1369
            R CA++MMYSG GYFGMQRNEGF+TIEG+++ A+ ++  I  E  K P  I FQRASKTDK VSA GQVC+ KL      +D +NS LP QIRILDVF+ TK F+ K     R Y+YL P+FAFS    I+ E    +R +  T  ++N +  +YKGTHNF NFTS+R P D SC RY+M++ C   F  +G  E+  I V GQSFMLHQIRKMIGL +A+V+G   E +FE VF+ +K+DIPKAPGLGL+LD+VNY  YN ++  +G  + I+W  Y+ QI++F+E +++ ++ + E E++SM +W++ L+ H+Y  R+
Sbjct:   57 RNCAMLMMYSGTGYFGMQRNEGFRTIEGELMSALYNSGYILEEHMKTPSMIHFQRASKTDKNVSAAGQVCAAKLPIDTLSIDKINSHLPDQIRILDVFRVTKKFNPKTACNYRVYQYLLPTFAFSFGGGITLENCWRYRLSAETLTRINELFRHYKGTHNFFNFTSRRTPDDRSCLRYIMSIECGPPFLLDGDREFAVITVQGQSFMLHQIRKMIGLVVAIVKGCATEAVFENVFKAEKMDIPKAPGLGLLLDRVNYTHYNKRFCSDGSHQSIDWERYKRQIDEFKEAYIFRHMVEREVEDNSMFEWMENLNNHAYGKRE 376
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A0X3Q5Z4 (tRNA pseudouridine synthase A OS=Schistocephalus solidus OX=70667 GN=TRUA PE=4 SV=1)

HSP 1 Score: 338.191 bits (866), Expect = 2.451e-108
Identity = 162/332 (48.80%), Postives = 233/332 (70.18%), Query Frame = 2 
Query:  383 LSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFND--VVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE-ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGI-EYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFR 1366
            L E+    + +R CAI+MMYSG  Y+GMQRN GF+TIEGD+L A+  + LI  +    P  I+FQRASKTDK VSA+GQVC+ K+  F+D   V+ +N  LP QIR+LDVF+ T+SF +K     RTY+Y+ P+FAF+ ++ ++ E    +R +  T   VN +L+++KGTHNF NFTS+R   D SCRRY+M++ C + F  +G  E+  I V GQSFMLHQIRKMIGL +A+V G+  E IFEK FQ ++LD+PKAPGLGL LDKV++  YN ++  +G   PI+W  Y+ +++ F+E+ ++ +I + E EE+SM++WL+ L  H++D+R
Sbjct:   44 LDENLPCAERRRNCAILMMYSGWDYYGMQRNAGFRTIEGDLLLALHKSGLISKDHLDNPFLIRFQRASKTDKNVSAVGQVCNAKIP-FSDESPVEKINEHLPQQIRVLDVFRVTQSFSSKTACNHRTYQYILPTFAFAARDSLTRESCWQYRISAETLTHVNNMLNHFKGTHNFFNFTSRRTANDLSCRRYIMSIECGAPFLLDGDREFAVITVVGQSFMLHQIRKMIGLVIAIVSGHTTEAIFEKAFQGERLDLPKAPGLGLFLDKVDFTRYNTRFCNDGSHHPIDWNAYKEKMDVFKEEHIFRHIVQKEVEENSMQEWLEGLCKHTFDYR 374
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A564Y4Y1 (PseudoU_synth_1 domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS2754 PE=4 SV=1)

HSP 1 Score: 337.035 bits (863), Expect = 2.483e-108
Identity = 161/320 (50.31%), Postives = 222/320 (69.38%), Query Frame = 2 
Query:  416 RMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE-ISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGI-EYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD 1369
            R CAI+M+YSG GY+GMQ+NEGFKTIEG++L A+ +A  I  E  K    I F RASKTDK VSA GQVC+ KL   N  VD +NS LP QIR+LDVF+ TK F+ K     R Y+YL P+FAF+P++ +   +  N+R    T  +VN +L  YKGTHNF NFTS R   D SC RY+M++ C S F  +G  E+  I + GQSFMLHQIRKMIGL + + +GY  E +FEKVF+ ++LD+P+APGLGL+LD+V +  YNN++  +   K I+W +Y+ QIE F+E++++ ++ K E E +SM +W+  L+LH++D R+
Sbjct:   56 RKCAILMIYSGTGYYGMQKNEGFKTIEGELLSALHNAGYITREHVKNQQLIHFSRASKTDKNVSAAGQVCAAKLPIDNLSVDKINSFLPDQIRVLDVFRVTKKFNPKTACNYRIYQYLIPTFAFAPRDSLKLSDCWNYRLPSKTLSRVNELLLRYKGTHNFFNFTSGRASDDKSCVRYIMSMECGSPFLLDGCREFAVISIQGQSFMLHQIRKMIGLVIGIAKGYATEDVFEKVFKTERLDVPRAPGLGLLLDQVCFTRYNNRFCADCSHKSIDWDMYKPQIEKFKEEYIFKHMVKCEVEGNSMFEWMSNLNLHTFDKRE 375
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A068WDG6 (tRNA pseudouridine synthase A mitochondrial OS=Echinococcus granulosus OX=6210 GN=EgrG_000889900 PE=4 SV=1)

HSP 1 Score: 332.798 bits (852), Expect = 1.458e-106
Identity = 161/327 (49.24%), Postives = 223/327 (68.20%), Query Frame = 2 
Query:  416 RMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSP-KEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKR-------LPTDSSCRRYLMNLTCESVFQENG-IEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD 1369
            R CA++MMYSG GYFGMQRNEGF+TIEG+++ A+ ++  I  E  K P  I FQRASKTDK VSA GQVC+ KL      +D +NS LP QIRILD+F+ TK F+ K +   R Y+YL P+FAFS    I+ E    +R +  T  ++N +  +YKGTHNF NFTS+R        P D SC RY+M++ C   F  +G  E+  I V GQSFMLHQIRKMIGL +A+V+G   E +FE VF+ +K+DIPKAPGLGL+LD+VNY  YN ++  +G  + I+W  Y+ QI++F+E +++ ++ + E E++SM +W++ L+ HSY  R+
Sbjct:   57 RNCAMLMMYSGTGYFGMQRNEGFRTIEGELMSALYNSGYILEEHMKTPSMIHFQRASKTDKNVSAAGQVCAAKLPIDTLSIDKINSHLPDQIRILDIFRVTKKFNPKTVCNYRVYQYLLPTFAFSSGGGITLENCWRYRLSAETLTRINELFRHYKGTHNFFNFTSRRQVSPVALTPDDKSCLRYIMSIECGPPFLLDGDREFAVITVQGQSFMLHQIRKMIGLVVAIVKGCATEAVFENVFKAEKMDIPKAPGLGLLLDQVNYTHYNKRFCSDGSHQSIDWERYKRQIDEFKEAYIFRHMVEREVEDNSMFEWMENLNKHSYGKRE 383
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Match: pus1 (pseudouridine synthase 1 [Source:NCBI gene;Acc:103043305])

HSP 1 Score: 296.975 bits (759), Expect = 8.306e-96
Identity = 144/345 (41.74%), Postives = 218/345 (63.19%), Query Frame = 2 
Query:  335 EESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYD 1360
            E    +K+  ++D +   E + PK   R   ++M YSG GY+GMQRN G   FKTIE +++ A++ A  I         K+ FQR ++TDK VSA GQV S+KL    D++  +N +LP QIR+L   + T  F++K     RTY Y+ P+ AFSPK+ ++E+  +FR +  T  KVNR+ + YKGTHNFHNFTS++ P D S +RY+ +++C   F    +E+  I V GQSFM+HQIRKMIGL +AVV+GY  E I E+ +  +K+D+PKAPGLGL+L++V+++ YN ++  +G  + + W   +  I  F++  +Y  I + E +E SM  W+ TL +H ++
Sbjct:   71 ENDHQVKKLKTDDDQTSDEKRFPK---RKVVLLMAYSGKGYYGMQRNCGNSQFKTIEDELVTALIKAGCIPENHGDDMKKMSFQRCARTDKGVSAAGQVVSLKLWLIEDILQKINKQLPSQIRVLGFKRVTGGFNSKNNCDARTYSYMLPTVAFSPKDHNQED-TSFRLDAETLQKVNRLFNLYKGTHNFHNFTSQKGPRDPSAKRYITHMSCGEPFIRKDVEFAEITVRGQSFMMHQIRKMIGLVIAVVKGYAGEQIIERSWGEEKVDVPKAPGLGLVLERVHFDRYNKRFGGDGLHETLEWTQEEDAIAAFKDAHIYPSIVETECQEKSMVSWMATLPIHDFE 411
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:103044309])

HSP 1 Score: 60.4622 bits (145), Expect = 1.069e-9
Identity = 65/272 (23.90%), Postives = 114/272 (41.91%), Query Frame = 2 
Query:  428 IMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFND--------VVDTLNSKLPLQ-IRILDVFQTTKSFHAKGMVKCRTY--RYLTPSFAFSPKEISEEELI-NFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLM-----NLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQ-KLDIPK---APGLGLILDKVNY 1180
            +   Y G  Y G+ +    + +EG  +Q  ++  +      K   ++    +S+TD  V AL     + + R +D        + D LN  L  + IRI   ++ +  FHA+     RTY  R  T   ++S   I+E++L    R  ++    +    +   GTH+F  F ++     +  +   M      L+         IEY  +    +S++  Q+R+M G  +AV +G       +K+   +  L  P+   AP  GL L KV Y
Sbjct:   10 LFFQYLGTKYSGVMKIPAHQPVEG--VQNHIENAV---RRLKPLNEVSLYISSRTDTGVHALCNSAHLDIQRKDDKPPFTGKVLTDALNLYLKKEPIRITRAYRVSNEFHARHSAISRTYVYRLATGLSSYSDMPITEKDLCWALRDTKLDISAMEEASAVLTGTHDFSTFQARGSDPKNPVKTMEMVQVQPGLSFSQRHFNRDIEYWELSFKCRSYLYKQVRRMTGALVAVGQGKLSVRQMQKLLDARDSLAYPQNMCAPACGLYLTKVEY 276
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:103044309])

HSP 1 Score: 57.3806 bits (137), Expect = 6.963e-9
Identity = 57/221 (25.79%), Postives = 95/221 (42.99%), Query Frame = 2 
Query:  581 ASKTDKTVSALGQVCSVKLSRFND--------VVDTLNSKLPLQ-IRILDVFQTTKSFHAKGMVKCRTY--RYLTPSFAFSPKEISEEELI-NFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLM-----NLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQ-KLDIPK---APGLGLILDKVNY 1180
            +S+TD  V AL     + + R +D        + D LN  L  + IRI   ++ +  FHA+     RTY  R  T   ++S   I+E++L    R  ++    +    +   GTH+F  F ++     +  +   M      L+         IEY  +    +S++  Q+R+M G  +AV +G       +K+   +  L  P+   AP  GL L KV Y
Sbjct:   34 SSRTDTGVHALCNSAHLDIQRKDDKPPFTGKVLTDALNLYLKKEPIRITRAYRVSNEFHARHSAISRTYVYRLATGLSSYSDMPITEKDLCWALRDTKLDISAMEEASAVLTGTHDFSTFQARGSDPKNPVKTMEMVQVQPGLSFSQRHFNRDIEYWELSFKCRSYLYKQVRRMTGALVAVGQGKLSVRQMQKLLDARDSLAYPQNMCAPACGLYLTKVEY 254
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Match: pus3 (pseudouridine synthase 3 [Source:NCBI gene;Acc:103046008])

HSP 1 Score: 56.225 bits (134), Expect = 3.822e-8
Identity = 64/285 (22.46%), Postives = 107/285 (37.54%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGY--FGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFN---------DVVDT--------------LNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSII--------VNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILD 1168
            +R  AI + Y G  Y  F +Q N    T+E  + +A+L   LI + +        + R  +TDK VSA  QV S+ L             D +D               LN  LP  IR L        F A+     RTYRY  P                    E+  + +      Y+GTH++ N    ++   +   ++   +   SV      E   +         V G +F+ HQ+R M+ + + + +      I +K+  ++  + P+ P   + +D
Sbjct:   75 RRHVAIRLAYLGWNYQGFAVQENTD-NTVEARLFEALLKTRLIQDRQSS-----NYHRCGRTDKGVSAYSQVISIDLRSCQYSGLGVTVPDGIDPKSNAPKEELPYVKILNRVLPPDIRALCWSPAETGFSARFDCVYRTYRYYFPR------------------GELDVELMATAARRYEGTHDYRNLC--KMDVGNGVLQFQRTIQSASVQPAQPCEPCPVNPLQLYVFEVKGLAFLYHQVRCMMAVLLLIGQKLEAPEIIDKLLDVE--NNPRKPQYSMAVD 331
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Match: pus3 (pseudouridine synthase 3 [Source:NCBI gene;Acc:103046008])

HSP 1 Score: 56.225 bits (134), Expect = 4.587e-8
Identity = 64/285 (22.46%), Postives = 107/285 (37.54%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGY--FGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFN---------DVVDT--------------LNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSII--------VNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILD 1168
            +R  AI + Y G  Y  F +Q N    T+E  + +A+L   LI + +        + R  +TDK VSA  QV S+ L             D +D               LN  LP  IR L        F A+     RTYRY  P                    E+  + +      Y+GTH++ N    ++   +   ++   +   SV      E   +         V G +F+ HQ+R M+ + + + +      I +K+  ++  + P+ P   + +D
Sbjct:  130 RRHVAIRLAYLGWNYQGFAVQENTD-NTVEARLFEALLKTRLIQDRQSS-----NYHRCGRTDKGVSAYSQVISIDLRSCQYSGLGVTVPDGIDPKSNAPKEELPYVKILNRVLPPDIRALCWSPAETGFSARFDCVYRTYRYYFPR------------------GELDVELMATAARRYEGTHDYRNLC--KMDVGNGVLQFQRTIQSASVQPAQPCEPCPVNPLQLYVFEVKGLAFLYHQVRCMMAVLLLIGQKLEAPEIIDKLLDVE--NNPRKPQYSMAVD 386
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005107.1 (pep scaffold:Pmarinus_7.0:GL480125:12705:18545:-1 gene:ENSPMAG00000004636.1 transcript:ENSPMAT00000005107.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 285.034 bits (728), Expect = 3.200e-93
Identity = 140/319 (43.89%), Postives = 203/319 (63.64%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPK-EISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            K+  A+++ YSG GY GMQRN G   ++TIE ++++A++ A  I +       K+ FQR ++TDK VSA+GQV S+K+   +DVV+ +N+ LP QIRI+ + + T  F++K +   RTY YL PSF F PK + + EE  +FR  E T   VN +L  Y GTHNFHNFTS +   ++S RRY+    C+    + GIE+  I + GQSFMLHQIRKM+GL +AV RG+  +   +  F   KLD+PKAPGLGL+L++V+Y+ Y+++YA +G    + W      + +F E+ VY+ I   E EE SM+ WL TL  H +
Sbjct:   11 KKKVALLVAYSGKGYHGMQRNAGNAAYRTIEDELVEALVRAGCIPDNHAADMRKMSFQRCARTDKGVSAIGQVVSLKMWLIDDVVNKINAHLPQQIRIMGMQRVTNGFNSKQLCDARTYIYLLPSFTFGPKGQAAWEE--SFRLPEETLSHVNALLRRYVGTHNFHNFTSGKGAQEASARRYVTEAYCQPPSLQQGIEFALIRIRGQSFMLHQIRKMVGLVVAVARGHAGDQRMQDAFGNDKLDVPKAPGLGLVLERVHYDRYDSRYAGDGVHAALAWPELAPALAEFAERHVYAGIEAAEREEGSMRRWLGTLDKHDF 327
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Yeast
Match: PUS1 (tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication [Source:SGD;Acc:S000006133])

HSP 1 Score: 110.538 bits (275), Expect = 1.881e-26
Identity = 59/166 (35.54%), Postives = 94/166 (56.63%), Query Frame = 2 
Query:  824 FRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVF--QENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEES 1315
            +R +     K     S Y G HNFHNFT  +   + S  R++ ++     F   +   E++SI ++GQSFMLHQIRKM+ +A  + R  C      + +  QK++IPKAP LGL+L+   +E YN +   + G K I++  YQ +++ F+ K +Y  I+K E +E+
Sbjct:  287 YRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKEPSAIRFMKDIKVSDPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERISQAYGQQKINIPKAPALGLLLEAPVFEGYNKRLE-QFGYKAIDFSKYQDEVDKFKMKHIYDKIYKEEVDEN 451

HSP 2 Score: 88.9669 bits (219), Expect = 2.343e-19
Identity = 51/139 (36.69%), Postives = 75/139 (53.96%), Query Frame = 2 
Query:  395 KKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKL-SRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAF-SPKEIS 805
            K+P+  KR  A+M+ Y G GY GMQ N    TIE  + +A ++A  I  +      K  F RA++TDK V A G + S+K+     D+   +N KLP  IR+ D+ +  K+F  + M   R Y YL P+++   PK  S
Sbjct:   68 KEPRLPKRKVAVMVGYCGTGYHGMQYNPPNPTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLISLKMIIEDPDIKQKINEKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGS 206
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Yeast
Match: PUS2 (Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification; mutation also affects pseudouridylation of some nuclear-encoded mRNAs; PUS2 has a paralog, PUS1, that arose from the whole genome duplication [Source:SGD;Acc:S000003031])

HSP 1 Score: 90.8929 bits (224), Expect = 1.646e-20
Identity = 45/118 (38.14%), Postives = 66/118 (55.93%), Query Frame = 2 
Query:  428 IMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSF 781
            +++ Y G GY+GMQ N   KTIEG++L  + D   I  E    P K  F  A++TDK V A+  + S+K++   D V  LN+ LP +IR+  +    K F+A+     R Y+YL P F
Sbjct:    1 MLLGYCGSGYYGMQYNPPHKTIEGEILTKLFDVGAISEENSLAPKKNSFMAAARTDKGVHAMLNLLSLKITLREDTVAKLNAALPPEIRVWGIQPVNKKFNARSACDSRWYQYLIPEF 118

HSP 2 Score: 82.4185 bits (202), Expect = 1.161e-17
Identity = 42/119 (35.29%), Postives = 63/119 (52.94%), Query Frame = 2 
Query:  845 FDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLD-IPKAPGLGLILDKVNYESYNNK 1198
             D     +  Y GTHNFHNFT+ +L  D S +R++  +    V  +    ++ + ++GQSFMLHQIR+M+ LA+   R      I    F       IP+AP  GL+L+   ++ YN K
Sbjct:  200 LDAFEAAMQEYVGTHNFHNFTTGKLWGDPSAQRHIKKV----VVSQASPGWICVRIHGQSFMLHQIRRMVALAVLAARCQLPPNIVRNYFNAGPRKYIPRAPAQGLLLEGPVFDGYNTK 314
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Yeast
Match: DEG1 (tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability [Source:SGD;Acc:S000001895])

HSP 1 Score: 62.7734 bits (151), Expect = 4.582e-11
Identity = 74/291 (25.43%), Postives = 120/291 (41.24%), Query Frame = 2 
Query:  353 KRKASEDIELLSESKKP-------KQCKRMCAIMMMYSGVGYFGMQRNEGF---KTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKL---------------SRFNDVVDTLNSKLPLQIRILDV-FQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAP 1147
            KRK S++    S++KK        K   R  A+   Y G  Y G+   + +    T+EG +L+AM   +L+ +   +     KF R  +TDK VSA+ QV S+++               SR    V  LN  LP  IRI  V  +   +F A+     R Y+Y+                  F    +  +K+++  SY+ G  +F NF   +L        +   +    +   +   Y   +V G +F+ HQ+R M+ +   V +      + E V +L   DI K P
Sbjct:   64 KRKISQEEVTRSKAKKAPKKFDFSKHNTRFIALRFAYLGWNYNGLAVQKEYTPLPTVEGTILEAMNKCKLVPSMVLQD---YKFSRCGRTDKGVSAMNQVISLEVRSNLTDEEQRDPTNDSREIPYVHVLNQLLPDDIRISAVCLRPPPNFDARFSCVHRHYKYI------------------FNGKNLNIEKMSKAASYFVGERDFRNFC--KLDGSKQITNFKRTIISSKILPLSETFYCFDLV-GSAFLWHQVRCMMAILFLVGQSL---EVPEIVLRLT--DIEKTP 325
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Nematostella
Match: EDO48717 (tRNA pseudouridine synthase [Source:UniProtKB/TrEMBL;Acc:A7RII9])

HSP 1 Score: 275.018 bits (702), Expect = 1.371e-89
Identity = 136/315 (43.17%), Postives = 201/315 (63.81%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            K+ CA+++ Y GVGY GMQ N G +TIE  ++ A+  AE+I ++  +   K+ FQR ++TDK VSA  QV S+K+S  + ++  +NS LP +IRI  + +TTK+F  K     RTY YLTP+FAF+   +S  E I         +++N+ LS YKGT N+HNFTS +   D S  RY+++  C   F+ NG E+V + V GQSF+LHQIRKMIGL +AVVRG    + F+ +      DIPKAP LGL+LD V++++YN K+  +G  +P++W +   ++  F+E F++ +I   E +E +M  WL TLS H+Y
Sbjct:    1 KKKCALLISYCGVGYAGMQINPGVRTIEEQLINALHQAEVITDDAKECLGKMSFQRCARTDKGVSAARQVVSLKISALSSLLPKINSFLPPEIRIFGMIKTTKAFDCKQNCSARTYEYLTPTFAFAKDYVSRTEKI---------EEINKALSNYKGTKNYHNFTSGKKFEDPSANRYIISFKCGKPFERNGREFVVLTVKGQSFVLHQIRKMIGLVIAVVRGVAPSSTFDLI------DIPKAPALGLVLDTVHFDAYNKKFGNDGLHEPLDWQLINEEVYKFKEDFIHKHIIDTETKEHTMMRWLNTLSSHTY 300
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Nematostella
Match: EDO36025 (tRNA pseudouridine synthase [Source:UniProtKB/TrEMBL;Acc:A7SJV8])

HSP 1 Score: 65.4698 bits (158), Expect = 2.008e-11
Identity = 64/286 (22.38%), Postives = 108/286 (37.76%), Query Frame = 2 
Query:  416 RMCAIMMMYSGVGYFGMQRNEGF-KTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFND--------------------------VVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTC---------------ESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAP 1147
            R  A+ + Y G    G    E    T+E  +  A++ A L+ + E        + R  +TDK VSA GQV S+ +    D                           VD LN  LP +IR+L        F A+     R Y+YL PS                    +  D +N      +G H+F NF   ++   +   ++  N+T                E+  ++ G E    ++ G +F+ HQ+R M+ + + V +G     + + +  ++K   P+ P
Sbjct:   80 RRIALRLSYLGWDMHGFASQEKIDNTVEAHLFDALIKACLVKSRE-----SCSYSRCGRTDKGVSAFGQVISLNVRTNLDKGPGVILRQESSVGRDTKGKTMKEILYVDILNKVLPKEIRVLAWAPVELDFDARFSCSYRKYKYLFPS------------------ANLDIDLMNLASKKLEGEHDFRNFC--KMDVANGVIQFTRNITSFKVQKVSNATYSAPGENKAKQRGYEMCEAVICGSAFLWHQVRCMMSILLMVGQGLEQPEVIDWLLDIEK--CPQRP 338
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Match: pus1 (pseudouridine synthase 1 [Source:NCBI gene;Acc:101161660])

HSP 1 Score: 294.664 bits (753), Expect = 3.531e-95
Identity = 143/338 (42.31%), Postives = 212/338 (62.72%), Query Frame = 2 
Query:  353 KRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEG---FKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY 1357
            KR   E+    +E K PK   +  A+++ YSG GY+GMQRN G   F+TIE D++ A++ +  I      +  K+ FQR ++TDK VSA GQV S+K+    DV++ +N  LP QIR+L + + T+ F++K     RTY Y+ P+ AFS K+     +  FR    T  +VNR+ S YKGTHNFHNFTS++ P+D S RRY+  ++C   F  N  E+  I V GQSFMLHQIRKMIGL +AV++GY  E + E+ +  +K+D+PKAPGLGL+L+ V+++ YN ++  +G  + + W      I+ F+E ++Y  I + E +E SM  W+ TL +H +
Sbjct:   57 KRIKVEEEHTENERKYPK---KKVALLLAYSGKGYYGMQRNPGTSQFRTIEDDLVSALVKSGCIPGNHGDEMKKMSFQRCARTDKGVSAAGQVVSLKVWLIEDVLEKINEHLPPQIRVLGLKRVTQGFNSKNNCDARTYSYMLPTVAFSQKDYDVGNIAAFRLQTETLQRVNRLFSLYKGTHNFHNFTSQKAPSDPSARRYITEMSCGEPFIRNNCEFAVINVRGQSFMLHQIRKMIGLVIAVIKGYAQEKVIERSWGEEKVDVPKAPGLGLVLEMVHFDRYNKRFGGDGLHERLEWDKQTEAIKAFKEAYIYPTIVETEIQEGSMVSWMSTLPIHDF 391
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Match: pus3 (pseudouridine synthase 3 [Source:NCBI gene;Acc:101173982])

HSP 1 Score: 64.3142 bits (155), Expect = 1.145e-10
Identity = 66/286 (23.08%), Postives = 113/286 (39.51%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGY--FGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFN--------------------------DVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDS-SCRRYLMNLTCESVF--QENGIEYVSII---VNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILD 1168
            +R  A+ + Y G  Y  F +Q N    T+E  + +A+L   LI + +        + R  +TDK VSA  QV ++ L                                V  LN  LP  IRILD     + F A+   + RTYRY  P  +                  +  D   R    Y+GTH+F N     +        R +++ T   ++  Q + ++  S+    V G +F+ HQ+R M+ + + + +   D  I  ++  ++  + P+ P   + +D
Sbjct:   74 RRHVALRLAYLGWAYQGFAVQENTD-NTVEARLFEALLKTRLIQDRQSS-----NYHRCGRTDKGVSAFSQVITIDLRSTQFCGGLGVTVPEKVDPSIKSKAASSELPYVKMLNRVLPQDIRILDCAPVAEGFSARFDCQSRTYRYYFPRGSLDVA--------------LMEDAAKR----YEGTHDFRNLCKMDVGNGVLQFERTILSATIRPMWSQQASSMDQYSLFVFEVKGLAFLYHQVRCMMAVLLLIGQKLEDPQIISQLLDVE--NNPRKPQYSMAVD 333
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Match: pus3 (pseudouridine synthase 3 [Source:NCBI gene;Acc:101173982])

HSP 1 Score: 64.3142 bits (155), Expect = 1.145e-10
Identity = 66/286 (23.08%), Postives = 113/286 (39.51%), Query Frame = 2 
Query:  413 KRMCAIMMMYSGVGY--FGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFN--------------------------DVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDS-SCRRYLMNLTCESVF--QENGIEYVSII---VNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILD 1168
            +R  A+ + Y G  Y  F +Q N    T+E  + +A+L   LI + +        + R  +TDK VSA  QV ++ L                                V  LN  LP  IRILD     + F A+   + RTYRY  P  +                  +  D   R    Y+GTH+F N     +        R +++ T   ++  Q + ++  S+    V G +F+ HQ+R M+ + + + +   D  I  ++  ++  + P+ P   + +D
Sbjct:   74 RRHVALRLAYLGWAYQGFAVQENTD-NTVEARLFEALLKTRLIQDRQSS-----NYHRCGRTDKGVSAFSQVITIDLRSTQFCGGLGVTVPEKVDPSIKSKAASSELPYVKMLNRVLPQDIRILDCAPVAEGFSARFDCQSRTYRYYFPRGSLDVA--------------LMEDAAKR----YEGTHDFRNLCKMDVGNGVLQFERTILSATIRPMWSQQASSMDQYSLFVFEVKGLAFLYHQVRCMMAVLLLIGQKLEDPQIISQLLDVE--NNPRKPQYSMAVD 333
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:101163566])

HSP 1 Score: 54.299 bits (129), Expect = 9.720e-8
Identity = 56/228 (24.56%), Postives = 97/228 (42.54%), Query Frame = 2 
Query:  581 ASKTDKTVSALGQVCSVKLSRFND--------VVDTLNSKLPL-QIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE-----ISEEELI-NFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENG---------IEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQ-KLDIPK---APGLGLILDKVNY 1180
            +S+TD  V AL       L R N+        +V+ LN  L   QIRI    +    FHA+   + RTY Y     A   +      ++E  L  + +  E+  + +    +   GTH+F +F  + + +D + +  +  L   S+   N          + ++ +    +SF+  Q+R+M G  +AV RG    +    + + +  L  P+   AP  GL L +V Y
Sbjct:   56 SSRTDTGVHALSNSAHFDLQRRNNKPPFAEDLLVEALNYHLQTEQIRINQAHRVADDFHARYRAQSRTYVY---RVALGVRHRTLLPLTESNLCWDIQDTELDVEAMREAAAVLIGTHDFSSF--RAVNSDMTFKNPVKTLDVASIQLGNSFASTYFHRQVSFLELTFTSRSFLYKQVRRMTGALVAVGRGLLTVSQLRDLLEAKDNLAYPQNLVAPAHGLFLTQVAY 278
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:101163566])

HSP 1 Score: 53.9138 bits (128), Expect = 1.161e-7
Identity = 56/228 (24.56%), Postives = 97/228 (42.54%), Query Frame = 2 
Query:  581 ASKTDKTVSALGQVCSVKLSRFND--------VVDTLNSKLPL-QIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKE-----ISEEELI-NFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENG---------IEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQ-KLDIPK---APGLGLILDKVNY 1180
            +S+TD  V AL       L R N+        +V+ LN  L   QIRI    +    FHA+   + RTY Y     A   +      ++E  L  + +  E+  + +    +   GTH+F +F  + + +D + +  +  L   S+   N          + ++ +    +SF+  Q+R+M G  +AV RG    +    + + +  L  P+   AP  GL L +V Y
Sbjct:   57 SSRTDTGVHALSNSAHFDLQRRNNKPPFAEDLLVEALNYHLQTEQIRINQAHRVADDFHARYRAQSRTYVY---RVALGVRHRTLLPLTESNLCWDIQDTELDVEAMREAAAVLIGTHDFSSF--RAVNSDMTFKNPVKTLDVASIQLGNSFASTYFHRQVSFLELTFTSRSFLYKQVRRMTGALVAVGRGLLTVSQLRDLLEAKDNLAYPQNLVAPAHGLFLTQVAY 279
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BLAST of tRNA pseudouridine synthase A vs. Planmine SMEST
Match: SMESG000074599.1 (SMESG000074599.1)

HSP 1 Score: 735.332 bits (1897), Expect = 0.000e+0
Identity = 353/354 (99.72%), Postives = 354/354 (100.00%), Query Frame = 2 
Query:  308 MPEIKNDILEESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD 1369
            MPEIKNDILEESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPI+WLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD
Sbjct:    1 MPEIKNDILEESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPIDWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD 354
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BLAST of tRNA pseudouridine synthase A vs. Planmine SMEST
Match: SMESG000074591.1 (SMESG000074591.1)

HSP 1 Score: 735.332 bits (1897), Expect = 0.000e+0
Identity = 353/354 (99.72%), Postives = 354/354 (100.00%), Query Frame = 2 
Query:  308 MPEIKNDILEESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD 1369
            MPEIKNDILEESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPI+WLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD
Sbjct:    1 MPEIKNDILEESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAVEGGRKPIDWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSYDFRD 354
[Close]
BLAST of tRNA pseudouridine synthase A vs. Planmine SMEST
Match: SMESG000063186.1 (SMESG000063186.1)

HSP 1 Score: 56.225 bits (134), Expect = 2.916e-8
Identity = 83/355 (23.38%), Postives = 139/355 (39.15%), Query Frame = 2 
Query:  338 ESFILKRKASEDIELLSESKKPKQCK--------------RMCAIMMMYSGVGYFGM-QRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCS------------------VKLSRFNDVVD-------TLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFTSKRLPTD-SSCRRYLMNLTCESVFQ-ENGIEYVSIIVN--GQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAV--EGGRKPINWLVYQSQIEDF 1264
            E+ IL+RK  E   L S+ K PKQ K              R   +   Y G  Y G+ Q+     T+   ++ A++  +LI++ E    +        +TDK VSA  QV S                  + +S+  ++ D        LN  LP  IRIL        F A+     R+Y+Y  P                   ++++ D +        G+H+F NF S ++    +   R +  +T E   + E   EY   ++N    SF+ HQIR  + + + + +    E + + +     LD+    GLG    K  Y+  ++   V  E   + INW   +  + D+
Sbjct:    8 ENEILRRKIKE---LQSQIKAPKQSKKNKSRKLEFSHYKMRHVVLKFCYLGWSYCGLAQQATVDNTVIEKIINALVLCKLIEDRETNIVI------CGRTDKGVSAFQQVISLDVRSALASGIGMIENSGINISQRKNIADEEIDYVFILNKVLPKDIRILAWSPVDVDFSARFNSSGRSYKYFFPK------------------SDLSIDLMQEAGKRLIGSHDFRNFCSMQISQGITQFVRNIYEVTIEPFDKSEADSEYRMYVINISASSFIYHQIRCTVAILLMIGQRKESEDVIDHL-----LDVEGIGGLG----KPFYQMASDLPLVFYEASYEGINWQTSEEAVNDY 326
[Close]
BLAST of tRNA pseudouridine synthase A vs. Planmine SMEST
Match: SMESG000041371.1 (SMESG000041371.1)

HSP 1 Score: 51.2174 bits (121), Expect = 9.660e-7
Identity = 75/298 (25.17%), Postives = 122/298 (40.94%), Query Frame = 2 
Query:  356 RKASEDIELLSESKKPKQCKRMCAIMMMYSGVGYFGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVSALGQVCSVKL-------------------SRFN------DVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRTYRYLTPSFAFSPKEISEE--ELINFRFNEITFDKVNRVLSYYKGTHNFHNFTS---KRLPTDSSCRRYLMNL-TCESVFQENGIEYVSIIVNGQSFMLHQIRKMIGLAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLG 1156
            RK S D    SE K      + C +   YSGV   G  +++    ++  + +++  ++L ++ +    + I F    +TDK VSA  QV S+ +                   +R N      D V  LN  LP  I+IL      K FHA      R+Y+Y  P    S + + E   +LI                    G+H+F NF +   K+  T  +   Y + +   + +  E+  +   I ++G SF+ HQIR ++G+ + +  G     I +     Q LDI    GLG
Sbjct:   44 RKYSLD---FSEYKLRHIALKFCYLGWRYSGV--IGQYQSDN--VVKSKLQESLFKSKLTEDRD----VNISF--CGRTDKGVSAFQQVISLVVRSNLVSGRGIIENSGININNRLNTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLI--------------------GSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEIID-----QLLDIDGIGGLG 303
The following BLAST results are available for this feature:
BLAST of tRNA pseudouridine synthase A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PUS13.985e-9843.29pseudouridine synthase 1 [Source:HGNC Symbol;Acc:H... [more]
PUS17.976e-9843.29pseudouridine synthase 1 [Source:HGNC Symbol;Acc:H... [more]
PUS11.840e-9743.29pseudouridine synthase 1 [Source:HGNC Symbol;Acc:H... [more]
PUS11.010e-8843.05pseudouridine synthase 1 [Source:HGNC Symbol;Acc:H... [more]
PUS15.892e-2144.86pseudouridine synthase 1 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pus-17.164e-6435.31tRNA pseudouridine synthase [Source:UniProtKB/TrE... [more]
tag-1247.373e-923.40Probable tRNA pseudouridine synthase tag-124 [Sou... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Pus11.002e-9244.69gene:FBgn0038811 transcript:FBtr0083944[more]
Pus13.468e-9244.69gene:FBgn0038811 transcript:FBtr0302323[more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pus17.241e-9541.92pseudouridine synthase 1 [Source:ZFIN;Acc:ZDB-GENE... [more]
pus12.528e-7943.13pseudouridine synthase 1 [Source:ZFIN;Acc:ZDB-GENE... [more]
pusl12.950e-823.66pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB... [more]
pusl13.012e-823.66pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PUS16.341e-10045.96pseudouridylate synthase 1 [Source:NCBI gene;Acc:1... [more]
ENSXETT00000026732.18.457e-7737.57tRNA pseudouridine synthase A, mitochondrial-like ... [more]
prkd34.262e-1527.65protein kinase D3 [Source:Xenbase;Acc:XB-GENE-6036... [more]
PUSL12.766e-923.29pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
PUSL14.860e-923.16pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pus11.180e-9843.20pseudouridine synthase 1 [Source:MGI Symbol;Acc:MG... [more]
Pus11.180e-9843.20pseudouridine synthase 1 [Source:MGI Symbol;Acc:MG... [more]
Pus12.529e-9842.90pseudouridine synthase 1 [Source:MGI Symbol;Acc:MG... [more]
Pus11.653e-9340.73pseudouridine synthase 1 [Source:MGI Symbol;Acc:MG... [more]
Pus12.035e-8037.91pseudouridine synthase 1 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9WU56|TRUA_MOUSE1.769e-9742.90tRNA pseudouridine synthase A OS=Mus musculus OX=1... [more]
sp|Q4KM92|TRUA_RAT1.929e-9742.90tRNA pseudouridine synthase A OS=Rattus norvegicus... [more]
sp|Q9Y606|TRUA_HUMAN8.836e-9743.29tRNA pseudouridine synthase A OS=Homo sapiens OX=9... [more]
sp|Q6FV05|PUS1_CANGA1.308e-4430.45tRNA pseudouridine synthase 1 OS=Candida glabrata ... [more]
sp|O74451|YCG6_SCHPO2.534e-3426.61Putative tRNA pseudouridine synthase C16C4.06c OS=... [more]
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BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3W6831.180e-11148.06tRNA pseudouridine synthase OS=Taenia asiatica OX=... [more]
A0A068Y8Z56.482e-10950.31tRNA pseudouridine synthase A mitochondrial OS=Ech... [more]
A0A0X3Q5Z42.451e-10848.80tRNA pseudouridine synthase A OS=Schistocephalus s... [more]
A0A564Y4Y12.483e-10850.31PseudoU_synth_1 domain-containing protein OS=Hymen... [more]
A0A068WDG61.458e-10649.24tRNA pseudouridine synthase A mitochondrial OS=Ech... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pus18.306e-9641.74pseudouridine synthase 1 [Source:NCBI gene;Acc:103... [more]
pusl11.069e-923.90pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
pusl16.963e-925.79pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
pus33.822e-822.46pseudouridine synthase 3 [Source:NCBI gene;Acc:103... [more]
pus34.587e-822.46pseudouridine synthase 3 [Source:NCBI gene;Acc:103... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000005107.13.200e-9343.89pep scaffold:Pmarinus_7.0:GL480125:12705:18545:-1 ... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
PUS11.881e-2635.54tRNA:pseudouridine synthase; introduces pseudourid... [more]
PUS21.646e-2038.14Mitochondrial tRNA:pseudouridine synthase; acts at... [more]
DEG14.582e-1125.43tRNA:pseudouridine synthase; introduces pseudourid... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO487171.371e-8943.17tRNA pseudouridine synthase [Source:UniProtKB/TrE... [more]
EDO360252.008e-1122.38tRNA pseudouridine synthase [Source:UniProtKB/TrE... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pus13.531e-9542.31pseudouridine synthase 1 [Source:NCBI gene;Acc:101... [more]
pus31.145e-1023.08pseudouridine synthase 3 [Source:NCBI gene;Acc:101... [more]
pus31.145e-1023.08pseudouridine synthase 3 [Source:NCBI gene;Acc:101... [more]
pusl19.720e-824.56pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
pusl11.161e-724.56pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
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BLAST of tRNA pseudouridine synthase A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000074599.10.000e+099.72SMESG000074599.1[more]
SMESG000074591.10.000e+099.72SMESG000074591.1[more]
SMESG000063186.12.916e-823.38SMESG000063186.1[more]
SMESG000041371.19.660e-725.17SMESG000041371.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017871 ID=SMED30017871|Name=tRNA pseudouridine synthase A|organism=Schmidtea mediterranea sexual|type=transcript|length=1449bp
TAGAAGCAAGTACAGTTGTATATTTTTTATAAATTAAATTGCAAACTTTC
TCTGTAGTTGGTGGTTGATTGTATGGAAAAATCTATTAAAAATTATAATC
CAACAAAAACTACGAAATTCTTACATTTTTTTCATAAATTATCAATCTCA
TCTATCGTTTTAACCACAGCTGCCATATTTATAAGTATATCTATTTCAGG
GTTCAAATATTACATTTATACGAAACCAAAGAAGCTTGAAGAACAAAAGC
TATACGAAGAGGCTGTTGCATTAAAAAAATCTGTTGAAAAAAAAGCACGT
ATTTAAAATGCCTGAAATTAAAAACGATATTTTAGAAGAATCTTTCATTC
TAAAACGCAAAGCTTCTGAAGACATAGAACTATTAAGTGAATCTAAAAAG
CCAAAACAATGTAAACGTATGTGTGCGATAATGATGATGTATTCTGGTGT
TGGTTACTTTGGAATGCAACGTAATGAAGGCTTTAAAACAATCGAAGGAG
ATGTTTTACAAGCTATGCTTGACGCTGAATTAATAGATAATGAAGAGTTT
AAGCAGCCTATGAAGATTAAATTTCAACGTGCATCAAAAACTGATAAAAC
TGTTTCAGCTTTAGGTCAAGTATGCTCAGTGAAATTATCCAGATTCAATG
ATGTTGTTGATACATTAAACAGTAAGTTACCACTTCAAATAAGAATATTG
GATGTATTTCAAACGACAAAAAGTTTCCATGCGAAAGGAATGGTTAAATG
CCGAACCTATCGATATTTAACTCCGAGTTTTGCTTTCTCTCCTAAGGAGA
TATCTGAAGAAGAATTGATAAATTTCCGATTTAACGAAATCACGTTTGAT
AAAGTGAATCGGGTATTATCATATTATAAAGGAACTCATAATTTCCACAA
TTTCACATCCAAGAGACTTCCTACCGATTCGAGTTGTAGGAGGTATCTGA
TGAATTTGACGTGTGAATCTGTATTTCAAGAAAACGGCATTGAGTATGTT
TCAATAATTGTCAACGGACAATCGTTTATGCTCCATCAGATAAGGAAAAT
GATAGGATTAGCCATGGCTGTAGTTAGGGGCTATTGCGATGAAACTATAT
TTGAAAAAGTATTTCAACTACAAAAGCTTGATATACCAAAGGCTCCCGGA
CTTGGATTAATTCTAGACAAAGTAAACTACGAATCATACAACAACAAGTA
CGCAGTAGAAGGTGGAAGAAAACCCATCAACTGGCTGGTTTATCAAAGTC
AAATTGAAGATTTCAGAGAGAAATTTGTCTATTCATATATTTTTAAAATT
GAAAATGAAGAATCTTCAATGAAGGATTGGTTGAAGACTTTGTCGTTGCA
TTCATACGATTTTAGGGATTTATAATTGATAAAAATAAAACATGTTATAA
AAATTAGTTGATTGTGATTTTATTAAATATATTTCCATCTTGACAAAAA
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protein sequence of SMED30017871-orf-1

>SMED30017871-orf-1 ID=SMED30017871-orf-1|Name=SMED30017871-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=356bp
MPEIKNDILEESFILKRKASEDIELLSESKKPKQCKRMCAIMMMYSGVGY
FGMQRNEGFKTIEGDVLQAMLDAELIDNEEFKQPMKIKFQRASKTDKTVS
ALGQVCSVKLSRFNDVVDTLNSKLPLQIRILDVFQTTKSFHAKGMVKCRT
YRYLTPSFAFSPKEISEEELINFRFNEITFDKVNRVLSYYKGTHNFHNFT
SKRLPTDSSCRRYLMNLTCESVFQENGIEYVSIIVNGQSFMLHQIRKMIG
LAMAVVRGYCDETIFEKVFQLQKLDIPKAPGLGLILDKVNYESYNNKYAV
EGGRKPINWLVYQSQIEDFREKFVYSYIFKIENEESSMKDWLKTLSLHSY
DFRDL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0031119tRNA pseudouridine synthesis
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
GO:0008033tRNA processing
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR001406PsdUridine_synth_TruA
IPR041708PUS1/PUS2-like
IPR029208COX14
IPR020094PsdUridine_synth_TruA_N
IPR020095PsdUridine_synth_TruA_C
IPR020097PsdUridine_synth_TruA_a/b_dom
IPR020103PsdUridine_synth_cat_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
GO:0016853isomerase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3DG3DSA:3.30.70.660coord: 147..355
e-value: 3.2E-75
score: 254.2
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 188..292
e-value: 5.4E-12
score: 46.1
NoneNo IPR availableGENE3DG3DSA:3.30.70.580coord: 27..146
e-value: 1.5E-32
score: 113.9
NoneNo IPR availablePANTHERPTHR11142:SF4TRNA PSEUDOURIDINE SYNTHASE Acoord: 22..351
IPR001406Pseudouridine synthase I, TruATIGRFAMTIGR00071TIGR00071coord: 39..286
e-value: 6.2E-43
score: 145.1
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 22..351
IPR041708Pseudouridine synthase PUS1/ PUS2-likeCDDcd02568PseudoU_synth_PUS1_PUS2coord: 41..291
e-value: 2.55084E-96
score: 284.506
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILYSSF55120Pseudouridine synthasecoord: 37..300