Calpain-5
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30017727 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Calpain-5 vs. Ensembl Human
Match: CAPN5 (calpain 5 [Source:HGNC Symbol;Acc:HGNC:1482]) HSP 1 Score: 157.532 bits (397), Expect = 1.444e-39 Identity = 168/649 (25.89%), Postives = 273/649 (42.06%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTK--ESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRDGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ L+ + R L+EDP FPA+ D L+ K + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + E + T Q M K G+ + + A+ + H Y +T ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC V+ S +++ K W+ W H D PR RG I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP+ T++ V+GA ++ + T N + I CE + S + T +P+++ +FYR
Sbjct: 12 NYSALRRDCRRRK---VLFEDPLFPATDDSLYYKGTP---GPAVRWKRPKGICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------NAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI--DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVI-----NTSHLSIHKT---WEEARLHGAWTLHED-PRQNRGGGCINHKDTFFQNPQYIFEVKKPE----DEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTG--ANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. Ensembl Human
Match: CAPN5 (calpain 5 [Source:HGNC Symbol;Acc:HGNC:1482]) HSP 1 Score: 157.532 bits (397), Expect = 1.444e-39 Identity = 168/649 (25.89%), Postives = 273/649 (42.06%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTK--ESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRDGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ L+ + R L+EDP FPA+ D L+ K + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + E + T Q M K G+ + + A+ + H Y +T ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC V+ S +++ K W+ W H D PR RG I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP+ T++ V+GA ++ + T N + I CE + S + T +P+++ +FYR
Sbjct: 12 NYSALRRDCRRRK---VLFEDPLFPATDDSLYYKGTP---GPAVRWKRPKGICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------NAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI--DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVI-----NTSHLSIHKT---WEEARLHGAWTLHED-PRQNRGGGCINHKDTFFQNPQYIFEVKKPE----DEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTG--ANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. Ensembl Human
Match: CAPN5 (calpain 5 [Source:HGNC Symbol;Acc:HGNC:1482]) HSP 1 Score: 157.532 bits (397), Expect = 1.444e-39 Identity = 168/649 (25.89%), Postives = 273/649 (42.06%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTK--ESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRDGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ L+ + R L+EDP FPA+ D L+ K + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + E + T Q M K G+ + + A+ + H Y +T ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC V+ S +++ K W+ W H D PR RG I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP+ T++ V+GA ++ + T N + I CE + S + T +P+++ +FYR
Sbjct: 12 NYSALRRDCRRRK---VLFEDPLFPATDDSLYYKGTP---GPAVRWKRPKGICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------NAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI--DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVI-----NTSHLSIHKT---WEEARLHGAWTLHED-PRQNRGGGCINHKDTFFQNPQYIFEVKKPE----DEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTG--ANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. Ensembl Human
Match: CAPN5 (calpain 5 [Source:HGNC Symbol;Acc:HGNC:1482]) HSP 1 Score: 140.198 bits (352), Expect = 8.663e-34 Identity = 168/685 (24.53%), Postives = 277/685 (40.44%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSK---------PNKEFISNDIIWRRPG-----QLSKLP-------------------C---LMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTK--ESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRDGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ L+ + R L+EDP FPA+ D L+ K ++ + + RPG ++ +P C + + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + E + T Q M K G+ + + A+ + H Y +T ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC V+ S +++ K W+ W H D PR RG I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP+ T++ V+GA ++ + T N + I CE + S + T +P+++ +FYR
Sbjct: 12 NYSALRRDCRRRK---VLFEDPLFPATDDSLYYKGTPGPAVRWKRPKYSGHSLAPHRPGWGDARRMENVPRPGSSGCPSSSLRARDLGICEDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------NAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI--DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVI-----NTSHLSIHKT---WEEARLHGAWTLHED-PRQNRGGGCINHKDTFFQNPQYIFEVKKPE----DEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGA--NSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYR 610
BLAST of Calpain-5 vs. Ensembl Human
Match: CAPN6 (calpain 6 [Source:HGNC Symbol;Acc:HGNC:1483]) HSP 1 Score: 137.117 bits (344), Expect = 6.158e-33 Identity = 164/651 (25.19%), Postives = 266/651 (40.86%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLK-ERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI--CKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFN--RN-SIIALAKKQLY-----WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQP-ETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWI-AAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEV----NIQLFCENKLG 2015
L+ DP F D LF + ++W+RP + P L+ N + + G+L + S L + + + +PN Q Q+ + Y G F F F G+ V+IDD LPT + S S++ NE F AL+EKAYAK LG Y L+ + I+DFTG L E++ K +++ E F + TK C IE P Q ++ H Y +T +I L L+ F + V ++RL + +E + + WQ+ T +K GL ++ E W+++E C FH N RN + +K+L W V D +R G G FL+N QY+ VP+ + + +++ Y +G N IG ELF+VE NR +R+H L + ++ + + R + ++ YL++G YV++P + G +++ L++F S+ V+ L D+P+ C YPK T+I+V A D+ + N + I C E S + +T+ +D +FYR D + N + FC+ LG
Sbjct: 26 LFCDPTFLPENDSLFYN---RLLPGKVVWKRPQDICDDPHLI-VGNISNHQLTQGRLGHKPMVSAFSCLAVQESHWTKTIPNHKEQEWDPQK-------TEKYAGIFHFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNE-FWNALLEKAYAKLLGCYEALDGLTITDIIVDFTGTLAETVDMQKGRYTELVEEKYKLFGELYKTFTKGGLICCSIESPNQEEQEVETDWGLLKGHTYTMTDIRKIRLG------ERLVEVFSAEK---VYMVRLRNPLGRQEWSGPWSEISEEWQQLTASDRKNLGLVMSDDGEFWMSLEDF-----CRN-FHKLNVCRNVNNPIFGRKELESVLGCWT-VDDDPLMNRSG---GCYNNRDTFLQNPQYIFTVPEDGHKVIMSLQQKDLRTYRRMGR-----PDNYIIGFELFKVEMNRKFRLHHL-YIQERAGTSTYIDTRTVFLSKYLKKGNYVLVPTMFQHGRTSEFLLRIF-SEVPVQLRELTLDMPKMS--------------------------------------------CWNLARGYPKVVTQITVHSAEDLEKKYANETVNPYLVIKCGKEEVRSPVQKNTVHAIFDTQAIFYRRTTDIPIIVQVWNSRKFCDQFLG 594
BLAST of Calpain-5 vs. Ensembl Celegans
Match: clp-7 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:U4PMH7]) HSP 1 Score: 111.309 bits (277), Expect = 6.904e-25 Identity = 131/528 (24.81%), Postives = 217/528 (41.10%), Query Frame = 3
Query: 75 LKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFH--PGKLSPFWLPILISLLKERN--MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPY---HVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS--LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLL----DISKCS----VLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQY-------LIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAG--NTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSS 1577
+ + E R D L+EDP FPA+ L+ K + IIW+RPG++ + P L+ + +RF G L WL ++ L R+ + +V P+ S + +Y G F F F R G+ V+IDDRLPT V L + S N EF AL+EKAYAK GGY L+ + A+ DFTG L E S +K T+ V M S C I+ N K + ++ H Y IT + N+ G VP++R+ + + E NG + W + +++ G++ E W++ + + + C+ V+ + + K++ W S D W+ G + N QY + V C + + +QKY ++T G N IG +++ + + +HD++ K + F R + + + G+YV++P A + L++FS+
Sbjct: 295 INFQQERQRCLDQRCLFEDPQFPANDSSLYYKTRPD---EPIIWKRPGEIYENPQLIV---GEKSRFDVKQGALGDCWLLAAVANLTLRDELFYRVVPPDQS--------------FTENYAGIFHFQFWRYGQWVDVVIDDRLPT-VDGRLCYMRSQENNEFWSALLEKAYAKLYGGYENLDGGTTAEALEDFTGGLTEFFDISKGDKS-----TTLAMLVRGMQMGSLFGCSID--ADENVKEAQLTNGLVRGHAYSITAIQTV-----------------NTYGGQVPLLRIRNPWGNSKEWNGAWSDGSSE-WSQVDPQQREQMGVQFAKDGEFWMSFDDFMTNFTQMECCNLSADVMDEISEMTGVEVHDKQKHQWVEKSEDGEWNSRQGTAGGCQNNDTYCNNPQYGTYFQVPMDSVEHDGKCTVIGAV--LQKY----RRELRTKGLDNLPIGFSVYKADGSGQ-AIHDVSGQKPIARTKVFINMREVTVRFRVPPGQYVIVPCTFDAHDDASFLLRIFSN 769
BLAST of Calpain-5 vs. Ensembl Celegans
Match: clp-7 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:U4PMH7]) HSP 1 Score: 111.309 bits (277), Expect = 6.904e-25 Identity = 131/528 (24.81%), Postives = 217/528 (41.10%), Query Frame = 3
Query: 75 LKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFH--PGKLSPFWLPILISLLKERN--MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPY---HVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS--LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLL----DISKCS----VLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQY-------LIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAG--NTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSS 1577
+ + E R D L+EDP FPA+ L+ K + IIW+RPG++ + P L+ + +RF G L WL ++ L R+ + +V P+ S + +Y G F F F R G+ V+IDDRLPT V L + S N EF AL+EKAYAK GGY L+ + A+ DFTG L E S +K T+ V M S C I+ N K + ++ H Y IT + N+ G VP++R+ + + E NG + W + +++ G++ E W++ + + + C+ V+ + + K++ W S D W+ G + N QY + V C + + +QKY ++T G N IG +++ + + +HD++ K + F R + + + G+YV++P A + L++FS+
Sbjct: 295 INFQQERQRCLDQRCLFEDPQFPANDSSLYYKTRPD---EPIIWKRPGEIYENPQLIV---GEKSRFDVKQGALGDCWLLAAVANLTLRDELFYRVVPPDQS--------------FTENYAGIFHFQFWRYGQWVDVVIDDRLPT-VDGRLCYMRSQENNEFWSALLEKAYAKLYGGYENLDGGTTAEALEDFTGGLTEFFDISKGDKS-----TTLAMLVRGMQMGSLFGCSID--ADENVKEAQLTNGLVRGHAYSITAIQTV-----------------NTYGGQVPLLRIRNPWGNSKEWNGAWSDGSSE-WSQVDPQQREQMGVQFAKDGEFWMSFDDFMTNFTQMECCNLSADVMDEISEMTGVEVHDKQKHQWVEKSEDGEWNSRQGTAGGCQNNDTYCNNPQYGTYFQVPMDSVEHDGKCTVIGAV--LQKY----RRELRTKGLDNLPIGFSVYKADGSGQ-AIHDVSGQKPIARTKVFINMREVTVRFRVPPGQYVIVPCTFDAHDDASFLLRIFSN 769
BLAST of Calpain-5 vs. Ensembl Celegans
Match: clp-6 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:Q9N4B2]) HSP 1 Score: 95.9005 bits (237), Expect = 3.258e-20 Identity = 124/489 (25.36%), Postives = 195/489 (39.88%), Query Frame = 3
Query: 120 LYEDPNFPAS-YDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKS-SPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENG-IIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLL----DISKCS----VLFHMFNRNSIIALAKKQLYWKYVSFDHFW-HRDGPRRGPKVIGQEFLKNNQY----LIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNT-QIGIELFEVEKNRLYRVHDLNHTKQVTCQTD-FQQGRYIVITAYLERGRYVVIP 1526
L+EDP FPA+ I + KP K + I+W RP ++ P L+ N G L W + L +R+ +F VVP +F + +Y G F F F R G+ V+IDDRLPT V+ L+ +SS + +E L+EKAYAK G Y L+ + A+ DFTG L E + +K + I + K + M S C I+ P+ + A H Y +TG + D V ++R+ + D E NG DK + W++ ++K E W+ ++ + ++ C+ V+ + I + K+ W S D W R G G F N QY P C ++ QKY N + + QIG+ ++++ ++ T T F R V+ + G YV++P
Sbjct: 157 LFEDPQFPANNSSIYYKKPPK----SHIVWLRPAKIFANPRLI-VNGKSRFDVRQGSLGNCWFLAAAATLAQRDELFYRVVPP---DQSFTE----------NYAGIFHFQFWRYGEWVDVVIDDRLPT-VNGKLIYMSSQDGDECWGPLLEKAYAKLYGTYEHLDGGTTTEALEDFTGGLTEFYDLTSTDKTM---ILAMIMKGMQMG--SLFACSIDPDPREKEAQLANGLVRGHAYSVTGVHTVETDKEK-----------------VALLRIRNPWGDTEWNGDWSDKSSL--WEQVDQEQREKMEFRIKEDGEFWMCLDDFMAQFANLDCCNLSADVMHEITEMTEIEVMEKQSKQWIQKSADGEWCSRKGTAGGCSNNENTFCTNPQYETYFRATSPSSNDKCT-VIAAVFQKYRR---NQLHRGLDMLQIGLSVYKMSGPNEKVTAEMMKTHAPIASTKLFVDYREAVVRFTVPPGYYVIVP 598
BLAST of Calpain-5 vs. Ensembl Celegans
Match: clp-4 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:Q8IAA9]) HSP 1 Score: 95.5153 bits (236), Expect = 4.495e-20 Identity = 104/371 (28.03%), Postives = 153/371 (41.24%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFH--PGKLSPFW-LPILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGE--SICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPY---HVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLL----DISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWH 1196
L+EDP FPA+ L+ + + IIW+RPG++ P L+ +RF G L W L L ++ +F +VP NQ+ + +Y G F F F GK V++DDRLPT V+ L + S +N EF AL+EKAYAK GGY L+ + A+ DFTG L E + KS VL+ V M S C I+ +N K + + H Y IT I D + L N + N + E NG + W K KK+ ++ E W++ E + C++ +F+ I + + V +H WH
Sbjct: 240 LFEDPEFPATAASLYYRTPPR---DRIIWKRPGEIIANPQLI---TQGESRFDVKQGALGDCWFLAALANITLYDALFYRIVP--PNQS-----------FTENYAGIFHFQFWHYGKWVDVVVDDRLPT-VNNQLYYLHSADNTEFWSALVEKAYAKLHGGYENLDGGTTAEALEDFTGGLTEYFDLRKSEKAAVLA-------ALVKGMEMGSLFGCSID--ADANIKEAQLRNGLVCGHAYSITAIHSITYYGEDTTLLRLRNPWGNEK---------------EWNGAWSDGSSE-WSKIDEATKKQIDVQFARDGEFWMSFEDFFSNFTQMEVCNLTAEIFDE--IAEMTGVNRATETVEEEHQWH 563
BLAST of Calpain-5 vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 95.9005 bits (237), Expect = 4.760e-20 Identity = 129/508 (25.39%), Postives = 213/508 (41.93%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILF--SKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFH--PGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQS-NKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLL----DISKCSVLFHMFNR-NSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVP------QPETCCIKMVLTNVQKY-----------VEVGANAIQTAGNT--QIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLE 1502
+F +L+ + L L+EDP F A+ LF +P K + W RPG++++ P L+ + +RF G+L WL + L ++ +F VVP +F + +Y G F F F + GK V+IDDRLPT+ + LL + S +N EF AL+EKAYAK G Y L+ + A+ D TG L E I D K ++ + M FG C IE P K S H Y ITG + D P+G + + IR ++ E NG + W+ VK+ GL+ ++ E W++ + + + C++ + + + + + W + D W R+ G + F N Q+ + + E C + + +QKY V +G A+ AGN ++ + F K+ + +N +++T + G Y+V+ + E
Sbjct: 281 DFYELRDQCLESK---RLFEDPQFLANDSSLFFSKRPPKR-----VEWLRPGEITREPQLI---TEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPP---DQSFTE----------NYAGIFHFQFWQYGKWVDVVIDDRLPTS-NGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFI----DLKNPPRNLMQMMMRGFEMGS-LFG-CSIEADPNVWEAKMSNGLVKGHAYSITGCRIV--DGPNGQ-------------TCILRIRNPWGNEQEWNGPWSDN-SREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTV--IFAVLQKYRRNLKQDGLDNVPIGF-AVYDAGNNRGRLSKQFFAANKSAMRSAAFIN-LREMTGRFRVPPGNYVVVPSTFE 737
BLAST of Calpain-5 vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086530) HSP 1 Score: 102.064 bits (253), Expect = 3.878e-22 Identity = 127/496 (25.60%), Postives = 205/496 (41.33%), Query Frame = 3
Query: 117 ALYEDPNFPASYDIL-FSK-PNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARF--HPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKS-SPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS--LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLY----------WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPE--------TCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP 1526
+L+EDP FPAS + L FS+ P++ I W RP ++++ P +F + +RF G+L WL +L +E N+F V+P + +F + +Y G F F F + GK VIIDDRLPT + L+ + S EF AL+EKAYAK G Y L+ S A+ DFTG + + D K ++ + QK + S C IE P + + + H Y IT I + P+ R +P+IR+ + ++ E NG + W+ K + GL + E W++ + L+ F+R I L+ L W+ ++ W G + F N QY+I + P+ T + ++ N + +G + T G + E+E NR ++ + V F R + L G Y+++P
Sbjct: 87 SLFEDPLFPASNESLQFSRRPDRH-----IEWLRPHEIAENP---QFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA---EQSFEE----------NYAGIFHFRFWQYGKWVDVIIDDRLPT-YNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTG----GVSEWYDLKEAPGNLFTILQKAAE--RNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPN-------------RQGKIPMIRMRNPWGNEAEWNGPWSDSSPE-WRYIPEEQKAEIGLTFDRDGEFWMSFQDFLN---------HFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECL-TIGFAIYSLNDRELE-NRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVP 529
BLAST of Calpain-5 vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0340532) HSP 1 Score: 101.293 bits (251), Expect = 1.513e-21 Identity = 127/496 (25.60%), Postives = 205/496 (41.33%), Query Frame = 3
Query: 117 ALYEDPNFPASYDIL-FSK-PNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARF--HPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKS-SPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS--LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLY----------WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPE--------TCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP 1526
+L+EDP FPAS + L FS+ P++ I W RP ++++ P +F + +RF G+L WL +L +E N+F V+P + +F + +Y G F F F + GK VIIDDRLPT + L+ + S EF AL+EKAYAK G Y L+ S A+ DFTG + + D K ++ + QK + S C IE P + + + H Y IT I + P+ R +P+IR+ + ++ E NG + W+ K + GL + E W++ + L + F+R I L+ L W+ ++ W G + F N QY+I + P+ T + ++ N + +G + T G + E+E NR ++ + V F R + L G Y+++P
Sbjct: 102 SLFEDPLFPASNESLQFSRRPDRH-----IEWLRPHEIAENP---QFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA---EQSFEE----------NYAGIFHFRFWQYGKWVDVIIDDRLPT-YNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTG----GVSEWYDLKEAPGNLFTILQKAAE--RNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPN-------------RQGKIPMIRMRNPWGNEAEWNGPWSDSSPE-WRYIPEEQKAEIGLTFDRDGEFWMSFQDFL---------NHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECL-TIGFAIYSLNDRELE-NRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVP 544
BLAST of Calpain-5 vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0112869) HSP 1 Score: 100.908 bits (250), Expect = 1.524e-21 Identity = 127/496 (25.60%), Postives = 205/496 (41.33%), Query Frame = 3
Query: 117 ALYEDPNFPASYDIL-FSK-PNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARF--HPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKS-SPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS--LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLY----------WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPE--------TCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP 1526
+L+EDP FPAS + L FS+ P++ I W RP ++++ P +F + +RF G+L WL +L +E N+F V+P + +F + +Y G F F F + GK VIIDDRLPT + L+ + S EF AL+EKAYAK G Y L+ S A+ DFTG + + D K ++ + QK + S C IE P + + + H Y IT I + P+ R +P+IR+ + ++ E NG + W+ K + GL + E W++ + L + F+R I L+ L W+ ++ W G + F N QY+I + P+ T + ++ N + +G + T G + E+E NR ++ + V F R + L G Y+++P
Sbjct: 87 SLFEDPLFPASNESLQFSRRPDRH-----IEWLRPHEIAENP---QFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA---EQSFEE----------NYAGIFHFRFWQYGKWVDVIIDDRLPT-YNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTG----GVSEWYDLKEAPGNLFTILQKAAE--RNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPN-------------RQGKIPMIRMRNPWGNEAEWNGPWSDSSPE-WRYIPEEQKAEIGLTFDRDGEFWMSFQDFL---------NHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECL-TIGFAIYSLNDRELE-NRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVP 529
BLAST of Calpain-5 vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086529) HSP 1 Score: 100.908 bits (250), Expect = 1.524e-21 Identity = 127/496 (25.60%), Postives = 205/496 (41.33%), Query Frame = 3
Query: 117 ALYEDPNFPASYDIL-FSK-PNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARF--HPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKS-SPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS--LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLY----------WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPE--------TCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP 1526
+L+EDP FPAS + L FS+ P++ I W RP ++++ P +F + +RF G+L WL +L +E N+F V+P + +F + +Y G F F F + GK VIIDDRLPT + L+ + S EF AL+EKAYAK G Y L+ S A+ DFTG + + D K ++ + QK + S C IE P + + + H Y IT I + P+ R +P+IR+ + ++ E NG + W+ K + GL + E W++ + L + F+R I L+ L W+ ++ W G + F N QY+I + P+ T + ++ N + +G + T G + E+E NR ++ + V F R + L G Y+++P
Sbjct: 87 SLFEDPLFPASNESLQFSRRPDRH-----IEWLRPHEIAENP---QFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPA---EQSFEE----------NYAGIFHFRFWQYGKWVDVIIDDRLPT-YNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTG----GVSEWYDLKEAPGNLFTILQKAAE--RNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPN-------------RQGKIPMIRMRNPWGNEAEWNGPWSDSSPE-WRYIPEEQKAEIGLTFDRDGEFWMSFQDFL---------NHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECL-TIGFAIYSLNDRELE-NRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVP 529
BLAST of Calpain-5 vs. Ensembl Fly
Match: CalpB (gene:FBgn0025866 transcript:FBtr0076343) HSP 1 Score: 93.2041 bits (230), Expect = 4.634e-19 Identity = 125/536 (23.32%), Postives = 220/536 (41.04%), Query Frame = 3
Query: 30 KMSDAVESQMSSPNFLKLKSELLRHPDGVALYEDPNFPAS-YDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARF--HPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKS-SPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSL--SDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLY----------WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPE--------TCCIKMVLTNVQKYVEVGANAIQ--------TAGNTQI---GIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYL---ERGRYVV 1520
K + A ++ +S +F L+ L + ++EDP+FPA+ +++S+ + W RPG ++ P +F + +RF G+L WL +L ++ +F V+P F + +Y G F F F + GK V+IDDRLPT + L+ + S EF AL+EKAYAK G Y L+ + A+ DFTG + + D K ++ S K + S C +E P + + + H Y IT + + P N +G L P+IR+ + +D E +G + W+ K++ GL + E W++ + L+ F+R I L+ L W+ F+ W G + + F N QY+I + P+ T + ++ N + VG + + T + Q+ G+ F+ + H +N T++V + G Y+++ + E G +++
Sbjct: 232 KATSARQNSVSKGDFQSLRDSCLAN---GTMFEDPDFPATNASLMYSRRPDRYYE----WLRPGDIADDP---QFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPP---DQDFQE----------NYAGIFHFKFWQYGKWVEVVIDDRLPT-YNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTG----GVTEWYDIKEAPPNLFSIMMKAAE--RGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTP------------NRQGKL-PMIRMRNPWGNDAEWSGPWSDSSPE-WRFIPEHTKEEIGLNFDRDGEFWMSFQDFLN---------HFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFIN-TREVCARFKLPPGHYLIVPSTFDPNEEGEFII 713
BLAST of Calpain-5 vs. Ensembl Zebrafish
Match: BX324225.2 (calpain 5b [Source:NCBI gene;Acc:100006332]) HSP 1 Score: 144.05 bits (362), Expect = 2.800e-35 Identity = 159/628 (25.32%), Postives = 262/628 (41.72%), Query Frame = 3
Query: 117 ALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITS---FYQKVVNM-TKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW-HRDGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
AL+EDP FPA D LF + N+ + W+RP +L P L F + SA H G+L W S L R ++++ V+P+ Q ++ + SY G F F F R G+ V+IDDRLPT V L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + + E ++ T+ +++V+ + ++ C I + ++ H Y +T ++ L + LL YF + + + +IR+ + +E + W+K + ++K G+ + E W+ E +L + N S +++ K W+ W HRD P R I + FL+N QY+ V + E VL +Q+ N IG ++ VE NR YR+H +Q + + R + + L+ GRYV+IP G ++ L+VF+ D+P C + + P C YP+ T++ V+ A ++ + + I+CE E S + T P +D +FYR
Sbjct: 25 ALFEDPLFPAVDDSLFYQGNR---IGRVHWKRPEELCDDPHL--FVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEK-------ADSYAGIFHFRFWRFGEWVDVVIDDRLPT-VDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPM--DLLEGQFAQDETARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRLG------HGLLAYFKSEK---LHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFEDFCHYFTDLILCRLIN-TSYLSIHKT---WEEEVMRGLWLHRDEPLRNRAGGCINHKTTFLQNPQYVFDVRKVE----DEVLICLQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHS---AQQKVAGSIYINSRCVFLRKELKEGRYVIIPTTFDPGQQGEFLLRVFT------------DVPSD--CKELTLDEP-------------------------------PQTCWTGMCGYPQLVTQVHVLSAEGLQGQDANGASDPYVIITCEGEKVRSPVHKDTRCPNFDIKGIFYR 572
BLAST of Calpain-5 vs. Ensembl Zebrafish
Match: capn5b (calpain 5b [Source:ZFIN;Acc:ZDB-GENE-091116-29]) HSP 1 Score: 143.665 bits (361), Expect = 3.041e-35 Identity = 159/628 (25.32%), Postives = 262/628 (41.72%), Query Frame = 3
Query: 117 ALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITS---FYQKVVNM-TKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW-HRDGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
AL+EDP FPA D LF + N+ + W+RP +L P L F + SA H G+L W S L R ++++ V+P+ Q ++ + SY G F F F R G+ V+IDDRLPT V L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + + E ++ T+ +++V+ + ++ C I + ++ H Y +T ++ L + LL YF + + + +IR+ + +E + W+K + ++K G+ + E W+ E +L + N S +++ K W+ W HRD P R I + FL+N QY+ V + E VL +Q+ N IG ++ VE NR YR+H +Q + + R + + L+ GRYV+IP G ++ L+VF+ D+P C + + P C YP+ T++ V+ A ++ + + I+CE E S + T P +D +FYR
Sbjct: 25 ALFEDPLFPAVDDSLFYQGNR---IGRVHWKRPEELCDDPHL--FVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEK-------ADSYAGIFHFRFWRFGEWVDVVIDDRLPT-VDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPM--DLLEGQFAQDETARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRLG------HGLLAYFKSEK---LHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFEDFCHYFTDLILCRLIN-TSYLSIHKT---WEEEVMRGLWLHRDEPLRNRAGGCINHKTTFLQNPQYVFDVRKVE----DEVLICLQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHS---AQQKVAGSIYINSRCVFLRKELKEGRYVIIPTTFDPGQQGEFLLRVFT------------DVPSD--CKELTLDEP-------------------------------PQTCWTGMCGYPQLVTQVHVLSAEGLQGQDANGASDPYVIITCEGEKVRSPVHKDTRCPNFDIKGIFYR 572
BLAST of Calpain-5 vs. Ensembl Zebrafish
Match: capn5a (calpain 5a [Source:ZFIN;Acc:ZDB-GENE-061103-214]) HSP 1 Score: 136.346 bits (342), Expect = 8.289e-33 Identity = 165/646 (25.54%), Postives = 265/646 (41.02%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI----CKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEVNIQLFCENKL 2012
L+EDP F A LF + N+ + W+RP L + P L F N SA + G+L W S L R +++ V+P+ +Q +P SY G F F F R G V+IDDRLPT + L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + K ++ + KV N + C I Q++ ++ H Y +T ++ L + LL +F + + S+ IR+ + E NG + W++ + +++ G+ + E W+ + +L + N S +++ K W+ W R D P R I + FL+N QY+ V + E VL +Q+ A N IG ++ VE NR+YR+H + ++ + + R + + L+ GRYV++P G + L++F +D + L D P Q T C C YPK T++ VI A ++ + + ISCE E S + T SP +D +FYR + IQ++ +N L
Sbjct: 26 LFEDPVFRAEDQSLFLQGNR---LGKVTWKRPKDLCEDPHL--FVNGISAHDLNQGQLGNCWFVAACSSLASREALWQKVIPDWKDQEWDKNKP-------ESYAGIFHFRFWRFGDWVDVVIDDRLPT-ANGKLIYCHSNDSNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPMDLLEGKFAQDEEARHQLFDRVLKVHN--RGGLISCSIRATTQADMEARLDCGLVKGHAYAVTDVRKVRLG------SGLLAFFKSEKLSM---IRMRNPWGQREWNGAWSDS-SEEWKRVSKGERERLGVTVEDDGEFWMDFDDFCKHFTDLILCRLIN-TSYLSIHKT---WEEEVMRGCWSRHDDPLRNRSGGCINHKTTFLQNPQYVFDVTKAE----DEVLICLQQQ---DKRARAKGENMAIGFDIQRVELNRIYRMHSI---QKSVGSSIYINSRSVFLRKDLKEGRYVIVPSTFDPGMPGDFLLRIF-TDVPSDCKELTVDEPPQTCWTSC---------------------------------------CG-----YPKLVTQVHVIRADGLKALDGDGSSDPYIIISCEGEKVCSPVHKDTQSPDFDVKAVFYR-KKPKQPIRIQIYNKNLL 586
BLAST of Calpain-5 vs. Ensembl Zebrafish
Match: capn5a (calpain 5a [Source:ZFIN;Acc:ZDB-GENE-061103-214]) HSP 1 Score: 136.346 bits (342), Expect = 8.289e-33 Identity = 165/646 (25.54%), Postives = 265/646 (41.02%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI----CKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEVNIQLFCENKL 2012
L+EDP F A LF + N+ + W+RP L + P L F N SA + G+L W S L R +++ V+P+ +Q +P SY G F F F R G V+IDDRLPT + L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + K ++ + KV N + C I Q++ ++ H Y +T ++ L + LL +F + + S+ IR+ + E NG + W++ + +++ G+ + E W+ + +L + N S +++ K W+ W R D P R I + FL+N QY+ V + E VL +Q+ A N IG ++ VE NR+YR+H + ++ + + R + + L+ GRYV++P G + L++F +D + L D P Q T C C YPK T++ VI A ++ + + ISCE E S + T SP +D +FYR + IQ++ +N L
Sbjct: 26 LFEDPVFRAEDQSLFLQGNR---LGKVTWKRPKDLCEDPHL--FVNGISAHDLNQGQLGNCWFVAACSSLASREALWQKVIPDWKDQEWDKNKP-------ESYAGIFHFRFWRFGDWVDVVIDDRLPT-ANGKLIYCHSNDSNEFWSALVEKAYAKMCGCYEALDGGNTADALVDFTGGVSEPMDLLEGKFAQDEEARHQLFDRVLKVHN--RGGLISCSIRATTQADMEARLDCGLVKGHAYAVTDVRKVRLG------SGLLAFFKSEKLSM---IRMRNPWGQREWNGAWSDS-SEEWKRVSKGERERLGVTVEDDGEFWMDFDDFCKHFTDLILCRLIN-TSYLSIHKT---WEEEVMRGCWSRHDDPLRNRSGGCINHKTTFLQNPQYVFDVTKAE----DEVLICLQQQ---DKRARAKGENMAIGFDIQRVELNRIYRMHSI---QKSVGSSIYINSRSVFLRKDLKEGRYVIVPSTFDPGMPGDFLLRIF-TDVPSDCKELTVDEPPQTCWTSC---------------------------------------CG-----YPKLVTQVHVIRADGLKALDGDGSSDPYIIISCEGEKVCSPVHKDTQSPDFDVKAVFYR-KKPKQPIRIQIYNKNLL 586
BLAST of Calpain-5 vs. Ensembl Zebrafish
Match: BX324225.2 (calpain 5b [Source:NCBI gene;Acc:100006332]) HSP 1 Score: 132.88 bits (333), Expect = 1.072e-31 Identity = 156/653 (23.89%), Postives = 261/653 (39.97%), Query Frame = 3
Query: 117 ALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQ--------------------------SARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITS---FYQKVVNM-TKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW-HRDGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
AL+EDP FPA D LF + N+ + W+RP S +P +N + + H G+L W S L R ++++ V+P+ Q ++ + SY G F F F R G+ V+IDDRLPT V L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + + E ++ T+ +++V+ + ++ C I + ++ H Y +T ++ L + LL YF + + + +IR+ + +E + W+K + ++K G+ + E W+ E +L + N S +++ K W+ W HRD P R I + FL+N QY+ V + E VL +Q+ N IG ++ VE NR YR+H +Q + + R + + L+ GRYV+IP G ++ L+VF+ D+P C + + P C YP+ T++ V+ A ++ + + I+CE E S + T P +D +FYR
Sbjct: 25 ALFEDPLFPAVDDSLFYQGNR---IGRVHWKRPEVSSIMPFSRSWNEARRRISFTDKHTSAELCDDPHLFVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEK-------ADSYAGIFHFRFWRFGEWVDVVIDDRLPT-VDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPM--DLLEGQFAQDETARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRLG------HGLLAYFKSEK---LHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFEDFCHYFTDLILCRLIN-TSYLSIHKT---WEEEVMRGLWLHRDEPLRNRAGGCINHKTTFLQNPQYVFDVRKVE----DEVLICLQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHS---AQQKVAGSIYINSRCVFLRKELKEGRYVIIPTTFDPGQQGEFLLRVFT------------DVPSD--CKELTLDEP-------------------------------PQTCWTGMCGYPQLVTQVHVLSAEGLQGQDANGASDPYVIITCEGEKVRSPVHKDTRCPNFDIKGIFYR 599
BLAST of Calpain-5 vs. Ensembl Xenopus
Match: ppa2 (pyrophosphatase (inorganic) 2 [Source:Xenbase;Acc:XB-GENE-961143]) HSP 1 Score: 139.428 bits (350), Expect = 5.523e-34 Identity = 142/527 (26.94%), Postives = 228/527 (43.26%), Query Frame = 3
Query: 48 ESQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLK-ERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI-CKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVE----KLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGP----RRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDN 1583
+ Q+ + N+ +LK E +++ L+EDP FPAS + LF + I W+RP +L + P L + S H G+L W S L ++ ++ V+ N Q + P Y G F F F G V+IDDRLP+ V +L+ SN EF AL+EKAYAK G Y L+ + AI+DFTG + ES D + T +Q+++ + + G+ + P S+ A +S H Y +T ++P V + F NS + + +IR+ + E + W K + K GL + E W+T E C +L S ++ K WK W ++ R G F +N QY+ V + E VL ++Q+ + N IG E+F+VE NR YR+H ++ ++ T ++ + R I + L GRYV+IP G + + L++F+ ++
Sbjct: 5 DKQLKTQNYHELKKECIKNG---KLFEDPEFPASDESLFYSKAPDL---RIEWKRPKELCEDPHLF-VDGISSHDLHQGRLGNCWFVAACSCLALKKALWTKVISNWKKQEWDPKRP-------HRYAGIFHFKFWCLGDWVDVVIDDRLPS-VDGNLVYCHSNVKNEFWSALLEKAYAKLAGSYEALDGGNTADAIVDFTGAVTESFELDKGDYRTSLMEQTKLFQELLKVYRAG-GLISCYIKPVSSNDMEAVTSYGLVKGHAYSVTAIKKMP-------VGQRILCFGNS--NKLFMIRMRNPWGKREWKGAWSDESEEWNKVSRSEKATLGLTVADDGEFWMTFEDWCLSFTHADVCRIL-----NTSYLSCYKT---WKQEEVHGAWSKNTDPLLNRSGGCFNETTFFQNPQYIFEVRKKED----EVLISLQQKDQRIHKKDGKGNNFIIGFEIFKVELNREYRIHKIS-GQEKTLRSSYINLRAIFLRKVLTHGRYVIIPTTFHPGISSSFILRLFTDES 493
BLAST of Calpain-5 vs. Ensembl Xenopus
Match: ppa2 (pyrophosphatase (inorganic) 2 [Source:Xenbase;Acc:XB-GENE-961143]) HSP 1 Score: 135.576 bits (340), Expect = 1.450e-32 Identity = 136/527 (25.81%), Postives = 220/527 (41.75%), Query Frame = 3
Query: 48 ESQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLK-ERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI-CKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVE----KLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGP----RRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDN 1583
+ Q+ + N+ +LK E +++ L+EDP FPAS + LF + I W+RP +L + P L + S H G+L W S L ++ ++ V+ N Q + P Y G F F F G V+IDDRLP+ V +L+ SN EF AL+EKAYAK G Y L+ + AI+DFTG + ES D + T +Q+++ + + G+ + P S+ A +S H Y +T ++P+ R++ + +W K + K GL + E W+T E C +L S ++ K WK W ++ R G F +N QY+ V + E VL ++Q+ + N IG E+F+VE NR YR+H ++ ++ T ++ + R I + L GRYV+IP G + + L++F+ ++
Sbjct: 5 DKQLKTQNYHELKKECIKNG---KLFEDPEFPASDESLFYSKAPDL---RIEWKRPKELCEDPHLF-VDGISSHDLHQGRLGNCWFVAACSCLALKKALWTKVISNWKKQEWDPKRPH-------RYAGIFHFKFWCLGDWVDVVIDDRLPS-VDGNLVYCHSNVKNEFWSALLEKAYAKLAGSYEALDGGNTADAIVDFTGAVTESFELDKGDYRTSLMEQTKLFQELLKVYRAG-GLISCYIKPVSSNDMEAVTSYGLVKGHAYSVTAIKKMPVGQ-----------------------RILCFG------------MTSWNKVSRSEKATLGLTVADDGEFWMTFEDWCLSFTHADVCRIL-----NTSYLSCYKT---WKQEEVHGAWSKNTDPLLNRSGGCFNETTFFQNPQYIFEVRKKE----DEVLISLQQKDQRIHKKDGKGNNFIIGFEIFKVELNREYRIHKIS-GQEKTLRSSYINLRAIFLRKVLTHGRYVIIPTTFHPGISSSFILRLFTDES 467
BLAST of Calpain-5 vs. Ensembl Xenopus
Match: icmt (isoprenylcysteine carboxyl methyltransferase [Source:Xenbase;Acc:XB-GENE-976857]) HSP 1 Score: 115.161 bits (287), Expect = 4.037e-26 Identity = 134/550 (24.36%), Postives = 230/550 (41.82%), Query Frame = 3
Query: 342 FELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLS-EHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRD---GPRRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
F +V+P+ Q + +P I Y G F F F + G+ V+IDDRLPT ++ L+ SN E AL+EKAYAK G Y L+ + A++DFTG + E I + V E +++V+ + I C I S+ ++ H Y +T ++ L + LL +F + + + +IR+ + + E NG + WQK + ++K G+ + E W+T E ++ + N S +++ K W+ W H+D G +L+N Q++ V +PE VL +Q+ + A N IG E+ +VE NR +R+H L + + + R + + L+ GRYV+IP G ++ L+VF+ D+P C C++K D T T +C YP+ +++ V+ A ++ + + + ISCE S ++ L P+++ +FYR
Sbjct: 10 FIVVIPDWKEQEWYADKPQI-------YAGIFHFHFWKFGEWVDVVIDDRLPT-INNQLIYCHSNEKNELWCALVEKAYAKLAGCYEALDGGNTADALVDFTGGVSEPIELTEGHFVTDVEKRNDLFERVLKVHNRGGLISCSIRATSASDMEARMACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LEMIRMRNPWGEREWNGPWSDSS-EEWQKVSKSEREKLGVTVEDDGEFWMTFEDFCKYYTDIIMCRLIN-TSYLSIHKT---WEEAVLRSSWVQHQDPLQNRSGGCLNFKATYLQNPQFVFDVKKPE----DEVLVCLQQKTKRTTRQEGKAENLAIGFEIHQVELNRRFRMHTL---QPKVASSIYINSRCVFLRTDLKEGRYVIIPTTFDPGHTGEFLLRVFT------------DLPSN--C--CELK-------------LDEPT-----------KTCWSGLCG-----YPQLVSQVHVVSAQGLKQQDSNGGADPYVIISCEGNKVQSPVIKDCLDPKFNVKGVFYR 485
BLAST of Calpain-5 vs. Ensembl Xenopus
Match: icmt (isoprenylcysteine carboxyl methyltransferase [Source:Xenbase;Acc:XB-GENE-976857]) HSP 1 Score: 111.694 bits (278), Expect = 8.628e-25 Identity = 133/547 (24.31%), Postives = 228/547 (41.68%), Query Frame = 3
Query: 351 VVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLS-EHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRDGPRR---GPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
V+P+ Q + +P I Y G F F F + G+ V+IDDRLPT ++ L+ SN E AL+EKAYAK G Y L+ + A++DFTG + E I + V E +++V+ + I C I S+ ++ H Y +T ++ L + LL +F + + + +IR+ + + E NG + WQK + ++K G+ + E W+T E ++ + N S +++ K W+ W H+D + G +L+N Q++ V +PE VL +Q+ + A N IG E+ +VE NR +R+H L + + + R + + L+ GRYV+IP G ++ L+VF+ D+P C C++K D T T +C YP+ +++ V+ A ++ + + ISCE S ++ L P+++ +FYR
Sbjct: 70 VIPDWKEQEWYADKPQI-------YAGIFHFHFWKFGEWVDVVIDDRLPT-INNQLIYCHSNEKNELWCALVEKAYAKLAGCYEALDGGNTADALVDFTGGVSEPIELTEGHFVTDVEKRNDLFERVLKVHNRGGLISCSIRATSASDMEARMACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LEMIRMRNPWGEREWNGPWSDSS-EEWQKVSKSEREKLGVTVEDDGEFWMTFEDFCKYYTDIIMCRLIN-TSYLSIHKT---WEEAVLRSSWVQHQDPLQNRSGGCLNFKATYLQNPQFVFDVKKPE----DEVLVCLQQKTKRTTRQEGKAENLAIGFEIHQVELNRRFRMHTL---QPKVASSIYINSRCVFLRTDLKEGRYVIIPTTFDPGHTGEFLLRVFT------------DLPSN--C--CELK-------------LDEPT-----------KTCWSGLCG-----YPQLVSQVHVVSAQGLKQQDSNGADPYVI-ISCEGNKVQSPVIKDCLDPKFNVKGVFYR 541
BLAST of Calpain-5 vs. Ensembl Xenopus
Match: icmt (isoprenylcysteine carboxyl methyltransferase [Source:Xenbase;Acc:XB-GENE-976857]) HSP 1 Score: 110.923 bits (276), Expect = 1.045e-24 Identity = 133/547 (24.31%), Postives = 227/547 (41.50%), Query Frame = 3
Query: 351 VVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLS-EHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRD---GPRRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
V+P+ Q + +P I Y G F F F + G+ V+IDDRLPT ++ L+ SN E AL+EKAYAK G Y L+ + A++DFTG + E I + V E +++V+ + I C I S+ ++ H Y +T ++ L + LL +F + + + +IR+ + + E NG + WQK + ++K G+ + E W+T E ++ + N S +++ K W+ W H+D G +L+N Q++ V +PE VL +Q+ + A N IG E+ +VE NR +R+H L + + + R + + L+ GRYV+IP G ++ L+VF+ D+P C C++K D T T +C YP+ +++ V+ A ++ + + ISCE S ++ L P+++ +FYR
Sbjct: 38 VIPDWKEQEWYADKPQI-------YAGIFHFHFWKFGEWVDVVIDDRLPT-INNQLIYCHSNEKNELWCALVEKAYAKLAGCYEALDGGNTADALVDFTGGVSEPIELTEGHFVTDVEKRNDLFERVLKVHNRGGLISCSIRATSASDMEARMACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LEMIRMRNPWGEREWNGPWSDSS-EEWQKVSKSEREKLGVTVEDDGEFWMTFEDFCKYYTDIIMCRLIN-TSYLSIHKT---WEEAVLRSSWVQHQDPLQNRSGGCLNFKATYLQNPQFVFDVKKPE----DEVLVCLQQKTKRTTRQEGKAENLAIGFEIHQVELNRRFRMHTL---QPKVASSIYINSRCVFLRTDLKEGRYVIIPTTFDPGHTGEFLLRVFT------------DLPSN--C--CELK-------------LDEPT-----------KTCWSGLCG-----YPQLVSQVHVVSAQGLKQQDSNGADPYVI-ISCEGNKVQSPVIKDCLDPKFNVKGVFYR 509
BLAST of Calpain-5 vs. Ensembl Mouse
Match: Capn5 (calpain 5 [Source:MGI Symbol;Acc:MGI:1100859]) HSP 1 Score: 160.614 bits (405), Expect = 9.380e-41 Identity = 163/646 (25.23%), Postives = 272/646 (42.11%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSE-HITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ LK LR L+EDP FPA+ D L+ K + W+RP + P L + S H G++ W S L R ++++ V+P+ Q ++P SY G F F F R G+ VI+DDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + + E +++V+ + G+ + + A+ + H Y +T ++ L + LL +F + + + +IRL + E + WQK + ++K G+ + E W+T E + DI KC ++ S +++ K W+ W R + P+ R I + F +N QY+ V +PE VL ++Q+ + N IG ++++VE+NR YR+H L H + + R + + L GRYV+IP G ++ L+VF+ D+P C ++ + P C YP++ ++ V+GA ++ + T N + I CE E S + T +P+++ +FYR
Sbjct: 12 NYSALKRACLRKK---VLFEDPLFPATDDSLYYKGTP---GPTVRWKRPKDICDDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKP-------DSYAGIFHFNFWRFGEWVDVIVDDRLPT-VNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRNQLFERVLKVHSRG-GLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLI-----NTSYLSIHKT---WEEARLHGAWTRHEDPQQNRSGGCINHKDTFFQNPQYVFEVKKPE----DEVLISIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRTCWSSLCGYPQQVAQVHVLGAAGLKDSPTG--ANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. Ensembl Mouse
Match: Capn5 (calpain 5 [Source:MGI Symbol;Acc:MGI:1100859]) HSP 1 Score: 160.614 bits (405), Expect = 9.380e-41 Identity = 163/646 (25.23%), Postives = 272/646 (42.11%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSE-HITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ LK LR L+EDP FPA+ D L+ K + W+RP + P L + S H G++ W S L R ++++ V+P+ Q ++P SY G F F F R G+ VI+DDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + + E +++V+ + G+ + + A+ + H Y +T ++ L + LL +F + + + +IRL + E + WQK + ++K G+ + E W+T E + DI KC ++ S +++ K W+ W R + P+ R I + F +N QY+ V +PE VL ++Q+ + N IG ++++VE+NR YR+H L H + + R + + L GRYV+IP G ++ L+VF+ D+P C ++ + P C YP++ ++ V+GA ++ + T N + I CE E S + T +P+++ +FYR
Sbjct: 12 NYSALKRACLRKK---VLFEDPLFPATDDSLYYKGTP---GPTVRWKRPKDICDDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKP-------DSYAGIFHFNFWRFGEWVDVIVDDRLPT-VNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRNQLFERVLKVHSRG-GLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLI-----NTSYLSIHKT---WEEARLHGAWTRHEDPQQNRSGGCINHKDTFFQNPQYVFEVKKPE----DEVLISIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRTCWSSLCGYPQQVAQVHVLGAAGLKDSPTG--ANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. Ensembl Mouse
Match: Capn6 (calpain 6 [Source:MGI Symbol;Acc:MGI:1100850]) HSP 1 Score: 138.658 bits (348), Expect = 1.690e-33 Identity = 168/665 (25.26%), Postives = 275/665 (41.35%), Query Frame = 3
Query: 72 FLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLK-ERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI--CKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFN--RN-SIIALAKKQLY-----WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQP-ETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWI-AAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEV----NIQLFCENKLG 2015
+ +LK E ++ DG L+ DP F D LF + ++W+RP +S P L+ N + + G+L + S L + + + +PN +Q ++P Y G F F F G+ V+IDD LPT + S S++ NE F AL+EKAYAK LG Y L+ + IMDFTG L E I K ++ E F + TK C IE P Q ++ L+ G+T D + + L +++ + ++RL + +E + + WQ+ T +K GL ++ E W+++E C FH N RN + +K+L W V D +R G G FL+N QY+ VP+ + + +++ Y +G N IG ELF+VE NR +R+H L + ++ + + R + ++ YL++G YV++P + G +++ L++F S+ V+ L D+P+ C YPK T+I+V A + + N + I C E S + +T+ +D +FYR D + N + FC+ LG
Sbjct: 13 YQELKQECMK--DG-RLFCDPTFLPENDSLFFN---RLLPGKVVWKRPQDISDDPHLI-VGNISNHQLIQGRLGNKAMISAFSCLAVQESHWTKAIPNHKDQEWDPRKP-------EKYAGIFHFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNE-FWNALLEKAYAKLLGCYEALDGLTITDIIMDFTGTLAEIIDMQKGRYTDLVEEKYKLFGELYKTFTKGGLICCSIESPSQEEQEVETDWG-----LLKGYTYTMTDIRKLRLGERLVEVFSTEK--LYMVRLRNPLGRQEWSGPWSEISEEWQQLTVTDRKNLGLVMSDDGEFWMSLEDF-----CHN-FHKLNVCRNVNNPVFGRKELESVVGCWT-VDDDPLMNRSG---GCYNNRDTFLQNPQYIFTVPEDGHKVIMSLQQKDLRTYRRMGR-----PDNYIIGFELFKVEMNRRFRLHHL-YIQERAGTSTYIDTRTVFLSKYLKKGSYVLVPTMFQHGRTSEFLLRIF-SEVPVQLRELTLDMPKMS--------------------------------------------CWNLARGYPKVVTQITVHSAEGLEKKYANETVNPYLIIKCGKEEVRSPVQKNTVHAIFDTQAIFYRRTTDIPIIIQVWNSRKFCDQFLG 594
BLAST of Calpain-5 vs. Ensembl Mouse
Match: Capn9 (calpain 9 [Source:MGI Symbol;Acc:MGI:1920897]) HSP 1 Score: 85.1149 bits (209), Expect = 1.921e-16 Identity = 78/278 (28.06%), Postives = 118/278 (42.45%), Query Frame = 3
Query: 36 SDAVESQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILF--SKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKE-SFGICQIEL--PPQSNKKSSAKSSPYHVYLITGFTEI 851
DA S +F +L+ L+ L+ED +FPAS LF +P F+ W+RPG++ + P + G+L WL + SL + VVP Y G F F F + + V+IDDRLPT L+ + S ++ EF AL+EKAYAK G Y L+ S + A+ DFTG + E+ + E F++ + K S C I+ +S ++S H Y +TG ++
Sbjct: 17 GDARTIHSSGQSFEQLRQGCLQSG---TLFEDADFPASNVSLFYSERPQVPFV-----WKRPGEIVEKPEFI-LGGATRTDICQGELGDCWLLAAIASLTLNQKALTRVVPQDQG-------------FGSGYAGIFHFQFWQHSEWLDVVIDDRLPT-FKDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENF-------QIREAPEDFFEILEKALKRGSLLGCSIDTLNASESEARTSLGLIKGHAYTVTGLDQV 264
BLAST of Calpain-5 vs. Ensembl Mouse
Match: Capn2 (calpain 2 [Source:MGI Symbol;Acc:MGI:88264]) HSP 1 Score: 83.9593 bits (206), Expect = 3.506e-16 Identity = 107/427 (25.06%), Postives = 161/427 (37.70%), Query Frame = 3
Query: 30 KMSDAVESQMSSPNFLKLKSELLRHP--DGVALYEDPNFPASYDILFSKPNKEFIS--NDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILIS--LLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSA--KSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLY------WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLI 1265
+ ++ + S + +L E LR+ + AL++DP+FPA L K + S I W+RP ++ P + G L WL I+ L E + +V P+ S Q +Y G F F F + G+ V++DDRLPT LL + S EF AL+EKAYAK G Y L + DFTG I + + + ++ QK + K S C I++ ++ ++ K H Y +TG E+ S GSL +IR+ + E G + +W V+ +G E W++ L ++R I L L WK D W R G + F N QYLI
Sbjct: 13 EAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDPSFPALPSSLGYKELGPYSSKTRGIEWKRPTEICADPQFI-IGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPPDQSFQE--------------NYAGIFHFQFWQYGEWVEVVVDDRLPTK-DGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTG----GIAEWYELRKPPPNLFKIIQKA--LEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEV-----------------ESSGSLQKLIRIRNPWGQVEWTGKWNDN-CPSWNTVDPEVRANLTERQEDG-EFWMSFSDFL---------RHYSRLEICNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389
BLAST of Calpain-5 vs. UniProt/SwissProt
Match: sp|O08688|CAN5_MOUSE (Calpain-5 OS=Mus musculus OX=10090 GN=Capn5 PE=1 SV=1) HSP 1 Score: 160.614 bits (405), Expect = 6.561e-40 Identity = 163/646 (25.23%), Postives = 272/646 (42.11%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSE-HITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ LK LR L+EDP FPA+ D L+ K + W+RP + P L + S H G++ W S L R ++++ V+P+ Q ++P SY G F F F R G+ VI+DDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + + E +++V+ + G+ + + A+ + H Y +T ++ L + LL +F + + + +IRL + E + WQK + ++K G+ + E W+T E + DI KC ++ S +++ K W+ W R + P+ R I + F +N QY+ V +PE VL ++Q+ + N IG ++++VE+NR YR+H L H + + R + + L GRYV+IP G ++ L+VF+ D+P C ++ + P C YP++ ++ V+GA ++ + T N + I CE E S + T +P+++ +FYR
Sbjct: 12 NYSALKRACLRKK---VLFEDPLFPATDDSLYYKGTP---GPTVRWKRPKDICDDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKP-------DSYAGIFHFNFWRFGEWVDVIVDDRLPT-VNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRNQLFERVLKVHSRG-GLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLI-----NTSYLSIHKT---WEEARLHGAWTRHEDPQQNRSGGCINHKDTFFQNPQYVFEVKKPE----DEVLISIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRTCWSSLCGYPQQVAQVHVLGAAGLKDSPTG--ANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. UniProt/SwissProt
Match: sp|O15484|CAN5_HUMAN (Calpain-5 OS=Homo sapiens OX=9606 GN=CAPN5 PE=1 SV=2) HSP 1 Score: 157.532 bits (397), Expect = 6.933e-39 Identity = 168/649 (25.89%), Postives = 273/649 (42.06%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTK--ESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRDGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ L+ + R L+EDP FPA+ D L+ K + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + E + T Q M K G+ + + A+ + H Y +T ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC V+ S +++ K W+ W H D PR RG I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP+ T++ V+GA ++ + T N + I CE + S + T +P+++ +FYR
Sbjct: 12 NYSALRRDCRRRK---VLFEDPLFPATDDSLYYKGTP---GPAVRWKRPKGICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------NAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPI--DLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADMEARLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVI-----NTSHLSIHKT---WEEARLHGAWTLHED-PRQNRGGGCINHKDTFFQNPQYIFEVKKPE----DEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTG--ANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. UniProt/SwissProt
Match: sp|Q8R4C0|CAN5_RAT (Calpain-5 OS=Rattus norvegicus OX=10116 GN=Capn5 PE=1 SV=1) HSP 1 Score: 157.147 bits (396), Expect = 9.652e-39 Identity = 165/656 (25.15%), Postives = 273/656 (41.62%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITS-FYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS----------LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ LK LR L+EDP+FPAS D L+ K + W+RP + P L + S H G++ W S L R ++++ V+P+ Q ++P SY G F F F R G+ V++DDRLPT V+ L+ SN+ EF A +EKAYAK G Y L+ + A++DFTG + E I + + E S +++V+ + G+ + + + + H Y +T ++ L + LL +F + + + +IRL + SD E WQK + ++K G+ + E W+T E + DI KC ++ S +++ K W+ W R + P+ R I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + L GRYV+IP G + L+VF+ D+P C ++ + P C YP++ T++ V+GA ++ ++T N + I CE E S + T +P+++ +FYR
Sbjct: 12 NYSALKRACLRRK---VLFEDPHFPASDDSLYYKGTP---GPTVRWKRPFDICDDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKP-------DSYAGIFHFNFWRFGEWVDVVVDDRLPT-VNNQLIYCHSNSKNEFWCAPVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRSQLFERVLKVHSRG-GLISASIKAMTAADMETRLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGERVWTGPWSDTSEE----------WQKVSKSEREKMGVTVQDDGEFWMTYEDMCRYFTDIIKCRLI-----NTSYLSIHKT---WEEARLRGAWTRHEDPQQNRSGGCINHKDTFFQNPQYIFEVKKPE----DEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---AASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGELLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRTCWSSLCGYPQQVTQVHVLGAAGLKDSSTG--ANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. UniProt/SwissProt
Match: sp|O35646|CAN6_MOUSE (Calpain-6 OS=Mus musculus OX=10090 GN=Capn6 PE=1 SV=2) HSP 1 Score: 138.658 bits (348), Expect = 1.182e-32 Identity = 168/665 (25.26%), Postives = 275/665 (41.35%), Query Frame = 3
Query: 72 FLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLK-ERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI--CKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFN--RN-SIIALAKKQLY-----WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQP-ETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWI-AAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEV----NIQLFCENKLG 2015
+ +LK E ++ DG L+ DP F D LF + ++W+RP +S P L+ N + + G+L + S L + + + +PN +Q ++P Y G F F F G+ V+IDD LPT + S S++ NE F AL+EKAYAK LG Y L+ + IMDFTG L E I K ++ E F + TK C IE P Q ++ L+ G+T D + + L +++ + ++RL + +E + + WQ+ T +K GL ++ E W+++E C FH N RN + +K+L W V D +R G G FL+N QY+ VP+ + + +++ Y +G N IG ELF+VE NR +R+H L + ++ + + R + ++ YL++G YV++P + G +++ L++F S+ V+ L D+P+ C YPK T+I+V A + + N + I C E S + +T+ +D +FYR D + N + FC+ LG
Sbjct: 13 YQELKQECMK--DG-RLFCDPTFLPENDSLFFN---RLLPGKVVWKRPQDISDDPHLI-VGNISNHQLIQGRLGNKAMISAFSCLAVQESHWTKAIPNHKDQEWDPRKP-------EKYAGIFHFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNE-FWNALLEKAYAKLLGCYEALDGLTITDIIMDFTGTLAEIIDMQKGRYTDLVEEKYKLFGELYKTFTKGGLICCSIESPSQEEQEVETDWG-----LLKGYTYTMTDIRKLRLGERLVEVFSTEK--LYMVRLRNPLGRQEWSGPWSEISEEWQQLTVTDRKNLGLVMSDDGEFWMSLEDF-----CHN-FHKLNVCRNVNNPVFGRKELESVVGCWT-VDDDPLMNRSG---GCYNNRDTFLQNPQYIFTVPEDGHKVIMSLQQKDLRTYRRMGR-----PDNYIIGFELFKVEMNRRFRLHHL-YIQERAGTSTYIDTRTVFLSKYLKKGSYVLVPTMFQHGRTSEFLLRIF-SEVPVQLRELTLDMPKMS--------------------------------------------CWNLARGYPKVVTQITVHSAEGLEKKYANETVNPYLIIKCGKEEVRSPVQKNTVHAIFDTQAIFYRRTTDIPIIIQVWNSRKFCDQFLG 594
BLAST of Calpain-5 vs. UniProt/SwissProt
Match: sp|O88501|CAN6_RAT (Calpain-6 OS=Rattus norvegicus OX=10116 GN=Capn6 PE=2 SV=1) HSP 1 Score: 137.502 bits (345), Expect = 2.539e-32 Identity = 167/665 (25.11%), Postives = 275/665 (41.35%), Query Frame = 3
Query: 72 FLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLK-ERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI--CKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFN--RN-SIIALAKKQLY-----WKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQP-ETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWI-AAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEV----NIQLFCENKLG 2015
+ +LK + ++ DG L+ DP F D LF + ++W+RP +S P L+ N + + G+L + S L + + + +PN Q ++P Y G F+F F G+ V+IDD LPT + S S++ NE F AL+EKAYAK LG Y L+ + IMDFTG L E I K ++ E F + TK C IE P Q ++ L+ G+T D + + L +++ + ++RL + +E + + WQ+ T +K GL ++ E W+++E C FH N RN + +K+L W V D +R G G FL+N QY+ VP+ + + +++ Y +G N IG ELF+VE NR +R+H L + ++ + + R + ++ YL++G YV++P + G +++ L++F S+ V+ L D+P+ C YPK T+I+V A + + N + I C E S + +T+ +D +FYR D + N + FC+ LG
Sbjct: 13 YQELKQDCMK--DG-RLFCDPTFLPENDSLFFN---RLLPGKVVWKRPQDISDDPHLI-VGNISNHQLIQGRLGNKAMISAFSCLAVQESHWTKAIPNHKEQEWDPRKP-------EKYAGIFRFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNE-FWNALLEKAYAKLLGCYEALDGLTITDIIMDFTGTLAEIIDMQKGRYTDLVEEKYKLFGELYKTFTKGGLISCSIESPSQEEQEVETDWG-----LLKGYTYTMTDIRKLRLGERLVEVFSTEK--LYMVRLRNPLGRQEWSGPWSEISEEWQQLTVTDRKNLGLVMSDDGEFWMSLEDF-----CHN-FHKLNVCRNVNNPVFGRKELESVVGCWT-VDDDPLMNRSG---GCYNNRDTFLQNPQYIFTVPEDGHKVIMSLQQKDLRTYRRMGR-----PDNYIIGFELFKVEMNRRFRLHHL-YIQERAGTSTYIDTRTVFLSKYLKKGNYVLVPTMFQHGRTSEFLLRIF-SEVPVQLRELTLDMPKMS--------------------------------------------CWNLARGYPKVVTQITVHSAEGLEKKYANETVNPYLTIKCGKEEVRSPVQKNTVHAIFDTQAIFYRRTTDIPIIIQVWNSRKFCDQFLG 594
BLAST of Calpain-5 vs. TrEMBL
Match: A0A091E4X5 (Calpain-5 OS=Fukomys damarensis OX=885580 GN=H920_00185 PE=3 SV=1) HSP 1 Score: 166.392 bits (420), Expect = 6.504e-39 Identity = 169/647 (26.12%), Postives = 276/647 (42.66%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSE-HITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ LK LR L+EDP+FPA+ D L+ K + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P I Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + + E +++V+ + G+ + + A+ H Y +T ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC +L S +++ K W+ W R + PR R I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YPK+ T++ V+GA ++ + T PN + I CE E S++ T P+++ +FYR
Sbjct: 128 NYSALKRACLRRK---VLFEDPHFPATDDSLYYK---GIPGPAVRWKRPKDICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPDI-------YAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDFANDEAKRNQLFERVLKVHSRG-GLISASIKAVTAADMEARLGCGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLL-----NTSYLSIHKT---WEEARLRGAWTRHEDPRQNRSGGCINHKDTFFQNPQYIFEVKKPE----DEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHK---VASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRTCWSSLCGYPKQVTQVHVLGAAGLKDSTTG--PNSYVIIKCEGEKVHSSVQKGTSMPEYNVKGIFYR 686
BLAST of Calpain-5 vs. TrEMBL
Match: A0A485NT48 (Calpain-5-like OS=Lynx pardinus OX=191816 GN=LYPA_23C014118 PE=3 SV=1) HSP 1 Score: 164.851 bits (416), Expect = 8.214e-39 Identity = 167/647 (25.81%), Postives = 277/647 (42.81%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSE-HITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ LK LR L+EDPNFPA+ D L+ K + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + E +++V+ + G+ + + A+ + H Y IT ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC V+ S +++ K W+ W R + PR R I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP++ T++ ++GAT ++ ++T N + I CE + S + T +P+++ +FYR
Sbjct: 12 NYSALKRACLRRK---VLFEDPNFPATDDSLYYKGTP---GPTVRWKRPKDICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------SAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRSEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGGFANDEAKRNQLFERVLKVHSRG-GLISASIKAVTAADMEARLACGLVKGHAYAITDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVI-----NTSYLSIHKT---WEEARLRGAWTRHEDPRKNRSGGCINHKDTFFQNPQYIFDVKKPE----DEVLICIQQRPKQSTRRDGKGENLAIGFDIYKVEENREYRMHGLQHR---AASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRSCWSSLCGYPQQVTQVHILGATGLKDSSTG--ANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. TrEMBL
Match: A0A337SKD2 (Calpain 5 OS=Felis catus OX=9685 GN=CAPN5 PE=3 SV=2) HSP 1 Score: 164.851 bits (416), Expect = 8.214e-39 Identity = 167/647 (25.81%), Postives = 277/647 (42.81%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSE-HITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGPR--RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ LK LR L+EDPNFPA+ D L+ K + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + E +++V+ + G+ + + A+ + H Y IT ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC V+ S +++ K W+ W R + PR R I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP++ T++ ++GAT ++ ++T N + I CE + S + T +P+++ +FYR
Sbjct: 12 NYSALKRACLRRK---VLFEDPNFPATDDSLYYKGTP---GPTVRWKRPKDICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------SAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRSEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGGFANDEAKRNQLFERVLKVHSRG-GLISASIKAVTAADMEARLACGLVKGHAYAITDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVI-----NTSYLSIHKT---WEEARLRGAWTRHEDPRKNRSGGCINHKDTFFQNPQYIFDVKKPE----DEVLICIQQRPKQSTRRDGKGENLAIGFDIYKVEENREYRMHGLQHR---AASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRSCWSSLCGYPQQVTQVHILGATGLKDSSTG--ANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. TrEMBL
Match: A0A1L5BWZ9 (Calpain-5 OS=Castor fiber OX=10185 GN=CAPN5 PE=2 SV=1) HSP 1 Score: 163.31 bits (412), Expect = 2.194e-38 Identity = 164/647 (25.35%), Postives = 278/647 (42.97%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITS-FYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW--HRDGPR-RGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+L LK LR L+EDP+FPA+ D L+ K + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ SN+ EF AL+EKAYAK G Y L+ + A++DFTG + E I + + + E S +++V+ + G+ + + + + H Y +T ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E + DI KC ++ S +++ K W+ W H D + R I + F +N QY+ V +PE VL +Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP++ T++ V+ AT ++ ++T N + I CE + S + T +P+++ +FYR
Sbjct: 12 NYLSLKRACLRRK---VLFEDPHFPATDDSLYYKGAP---GPTVRWKRPKDICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------DAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLINDETKRSQLFERVLKVHSRG-GLISASIKAMTAADMETRLACGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRLI-----NTSYLSIHKT---WEEARLHGAWTQHEDPQKNRSGGCINHKDTFFQNPQYIFEVKKPE----DEVLICIQQRPKRSTRQEGKGENLAIGFDIYKVEENRQYRMHSLQHK---VASSIYINSRSVFLRTEQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRTCWSSLCGYPRQVTQVHVLAATGLKDSSTG--ANSYVIIKCEGDKVCSAVQKGTSTPEYNVKGIFYR 570
BLAST of Calpain-5 vs. TrEMBL
Match: L8IUH0 (Calpain-5 (Fragment) OS=Bos mutus OX=72004 GN=M91_05035 PE=3 SV=1) HSP 1 Score: 163.31 bits (412), Expect = 2.404e-38 Identity = 166/647 (25.66%), Postives = 279/647 (43.12%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSE-HITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKL----LDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGP---RRGPKVIGQE-FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
N+ LK LR L+EDPNFPA+ D L+ K + + W+RP + + P L + S H G++ W S L R ++++ V+P+ Q ++P +Y G F F F R G+ V+IDDRLPT V+ L+ S++ EF AL+EKAYAK G Y L+ + A++DFTG + E I + + E +++V+ + G+ + + A+ + H Y IT ++ L + LL +F + + + +IRL + + E NG + WQK + ++K G+ + E W+T E L DI KC ++ S +++ K W+ W R + P R G + ++ F +N QY+ V +PE VL ++Q+ + N IG ++++VE+NR YR+H L H + + R + + GRYV+IP G ++ L+VF+ D+P C ++ + P C YP++ T++ V+GA ++ + T N + I CE + S + T +P++D +FYR
Sbjct: 18 NYSALKRACLRRK---VLFEDPNFPATDDSLYYKGSP---GPTVRWKRPKDICEDPRLF-VDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKP-------NAYAGIFHFHFWRFGEWVDVVIDDRLPT-VNNQLIYCHSSSRNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDFANDEAKRNQLFERVLKVHSRG-GLISASIKAVTAADMEARLACGLVKGHAYAITDVRKVRLG------HGLLAFFKSEK---LDMIRLRNPWGEREWNGPWSD-TSEEWQKVSKSEREKLGVTVQDDGEFWMTFEDLCRYFTDIIKCRLI-----NTSYLSIHKT---WEEARLRGAWTRHEDPLQNRSGGCINHKDSFFQNPQYIFDVKKPE----DEVLISIQQRPKQSTRRDGKGENLAIGFDIYKVEENRQYRMHSLQHR---AASSIYINSRSVFLRTEQPEGRYVIIPTTFEPGHTGEFLLRVFT------------DVPSN--CRELRLDEP-------------------------------PRTCWSSLCGYPQQVTQVHVLGAAGLKDSPTGA--NSYVIIKCEGDKVRSAVQKGTSTPEYDVKGVFYR 576
BLAST of Calpain-5 vs. Ensembl Cavefish
Match: capn5a (calpain 5 [Source:NCBI gene;Acc:103038917]) HSP 1 Score: 144.821 bits (364), Expect = 1.101e-35 Identity = 167/646 (25.85%), Postives = 269/646 (41.64%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLS---EHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEVNIQLFCENKL 2012
L+EDP FP + LF + N+ + W+RP +L P L F N SA H G+L W S L R +++ V+P+ +Q + P SY G F F F G+ V+IDDRLPT + +L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + + E E + +V+ + I C I Q++ ++ H Y +T + L + LL YF + + + +IR+ + + E NG + WQK + ++K G+ + E W+ E +L + N S +++ K W+ W R D P R I + +L+N QY+ V +PE VL +Q+ N IG +L VE NR+YR+H ++Q C + + R + + L+ GRYV+IP G + L+VF+ D+P C + + P C YP+ T++ V+ ++ + + + ISCE S S LSP++D LFYR ++I+++ +N L
Sbjct: 26 LFEDPLFPPVDESLFYQTNQ---IGKVTWKRPKELCSDPHL--FVNGISAHDLHQGRLGNCWFVAACSSLASREALWQKVIPDWKDQEWDSNHP-------DSYAGIFHFRFWLFGEWVDVVIDDRLPT-ANGNLIYCHSNDSNEFWSALVEKAYAKIHGCYEALDGGNTADALVDFTGGVSEPV--DLLEGRFGQEEEARNQLFDRVLKVHNRGGLISCSIRATTQADMEARLDCGLVKGHAYAVTDVRRVRLG------HGLLAYFKSEK---LMMIRMRNPWGEREWNGPWSD-SSEEWQKVSKGEREKLGVTVQDDGEFWMMFEDFSKHFTDLILCRLIN-TSYLSIHKT---WEEAVMMGSWSRHDDPLSNRSGGCINNKTTYLQNPQYVFDVKKPE----DEVLICLQQKDRRARPKEGKGENLAIGFDLQRVELNRVYRMHT---SQQKLCSSVYINSRSVFVRKDLKEGRYVIIPTTFNPGLQGDFLLRVFT------------DVPSD--CKELTLDEP-------------------------------PQTCWTGLCGYPQLVTQVHVMKGVGLQGQDSDGSSDPYIIISCEGVKVKSPAHKSNLSPEFDVKALFYR-KKPGQGIHIEVYNKNVL 589
BLAST of Calpain-5 vs. Ensembl Cavefish
Match: capn5b (calpain-5-like [Source:NCBI gene;Acc:103035636]) HSP 1 Score: 135.191 bits (339), Expect = 1.822e-32 Identity = 154/627 (24.56%), Postives = 261/627 (41.63%), Query Frame = 3
Query: 117 ALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHIT--SFYQKVVNM-TKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW-HRDGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
L+EDP FPA D LF + N+ + W+RP +L P L F + SA H G+L W S L R ++++ V+P+ Q ++ SY G F F F R G+ V+IDDRLPT V L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + ++ ++ + + +++V+ + ++ C I + ++ H Y +T ++ L + LL +F + + + +IR+ + +E + W+K + ++K G+ + E W+ + +L + N S +++ K W+ W +RD P R I + FL+N QY+ V + E VL +Q+ + N IG ++ VE NR YR+H +Q + + R + + L+ GRYV+IP G ++ L+VF T + D E L P P C YP+ T++ V+ A ++ + + + I+CE E S + T P +D +F+R
Sbjct: 25 TLFEDPLFPAVDDSLFYQGNR---IGRVHWKRPRELCDDPHL--FVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWREQEWDPEK-------DESYVGIFHFRFWRFGEWVDVVIDDRLPT-VDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPM-DLLEGQMAQDEVARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVKKVRLG------HGLLAFFKSEK---LHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFDDFCQYFTDLILCRLIN-TSYLSIHKT---WEEEVMRGTWVYRDDPLRNRSGGCINHKATFLQNPQYVFDVRKVE----DEVLICLQQKEKRATPKEGKGENLAIGFDIHRVELNRKYRMHS---AQQKVAGSIYINSRCVFLRKELKEGRYVIIPTTFDPGQQGEFLLRVF--------TDVPSDCKELTLDDP-------------------------------------PQTCWTGMCGYPQLVTQVHVLSAEGLQGQDSNGASDPYVIITCEGEKVRSPVHKDTRCPNFDVKGVFFR 572
BLAST of Calpain-5 vs. Ensembl Cavefish
Match: capn5b (calpain-5-like [Source:NCBI gene;Acc:103035636]) HSP 1 Score: 134.42 bits (337), Expect = 2.299e-32 Identity = 154/626 (24.60%), Postives = 261/626 (41.69%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHIT--SFYQKVVNM-TKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW-HRDGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
L+EDP FPA D LF + N+ + W+RP +L P L F + SA H G+L W S L R ++++ V+P+ Q ++ SY G F F F R G+ V+IDDRLPT V L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + ++ ++ + + +++V+ + ++ C I + ++ H Y +T ++ L + LL +F + + + +IR+ + +E + W+K + ++K G+ + E W+ + +L + N S +++ K W+ W +RD P R I + FL+N QY+ V + E VL +Q+ + N IG ++ VE NR YR+H +Q + + R + + L+ GRYV+IP G ++ L+VF T + D E L P P C YP+ T++ V+ A ++ + + + I+CE E S + T P +D +F+R
Sbjct: 26 LFEDPLFPAVDDSLFYQGNR---IGRVHWKRPRELCDDPHL--FVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWREQEWDPEK-------DESYVGIFHFRFWRFGEWVDVVIDDRLPT-VDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPM-DLLEGQMAQDEVARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVKKVRLG------HGLLAFFKSEK---LHMIRMRNPWGEKEWSGPWSDSSEEWKKVSKSEREKLGVTVQDDGEFWMNFDDFCQYFTDLILCRLIN-TSYLSIHKT---WEEEVMRGTWVYRDDPLRNRSGGCINHKATFLQNPQYVFDVRKVE----DEVLICLQQKEKRATPKEGKGENLAIGFDIHRVELNRKYRMHS---AQQKVAGSIYINSRCVFLRKELKEGRYVIIPTTFDPGQQGEFLLRVF--------TDVPSDCKELTLDDP-------------------------------------PQTCWTGMCGYPQLVTQVHVLSAEGLQGQDSNGASDPYVIITCEGEKVRSPVHKDTRCPNFDVKGVFFR 572
BLAST of Calpain-5 vs. Ensembl Cavefish
Match: ENSAMXT00000048279.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000063.1:113453:132090:1 gene:ENSAMXG00000038709.1 transcript:ENSAMXT00000048279.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 126.331 bits (316), Expect = 1.061e-29 Identity = 151/641 (23.56%), Postives = 257/641 (40.09%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDE-KVLSEHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEE--NGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRR----GPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
++ +LK E L+ L+EDP F S LF + + W+RPG++ + P L F SA + G++ W S L + +++ V+PN Q P Y G F F F G+ V++DDRLPT ++ L+ S +N EF AL+EKAYAK G Y L + A++DF+G + E + + S+ + ++N+ + I C I P + + + H Y +TG + L + LL YF N V +IR+ + E D++ L K + C N D+ C ++ S+++L K+ + + V F + P R G Q FL+N QY++ + + + + +++ + G N IG +F+VE NR YR+HD+ T+Q + + R + + L +GRY++IP G + L+V+++ + + C + + + R + F YP+ T++ V GA + + + + ISCE S + T PQ+D +FYR
Sbjct: 10 HYSELKRECLQQK---KLFEDPEFLCSNRSLFYRTPP---PGPVQWKRPGEMCEAPRL--FVEGISAHDLNQGEVGNCWFVAACSCLALKPQLWQKVIPNWKEQEWDPDRP-------DRYTGIFHFQFWIFGEWTDVVVDDRLPT-INGKLIYCHSKSNNEFWSALLEKAYAKLSGCYESLNGGNTGDAVVDFSGAVNEPVSLETGSFRTDSKTREKLFSDLLNVNDQGGIISCSIASPHERELRLANGLVKGHAYSVTGVRRVRLG------HGLLAYFKND---TVDLIRMRNPWGKHEWRGAWSDRWGEFLSDDFKLLKKMSFDDWCQN----------FTDVDVCRLM-----NTSVLSLQKR--WREVVHFGSWIKHSEPLRNRSGGCMNHRQTFLQNPQYILDLQDEDELLVSLQQRDMKIHRAHGQGE-----NLTIGFAIFKVELNRKYRMHDI-ITQQNVATSSYINARSVFLRKLLPKGRYIIIPSTFTPGILGDFMLRVYANQDLI--------------CRELDVDKPSVRCWSSFMG-------------------------------YPQVVTQVYVHGAEGLENQDS---ADPYVIISCEKNSIQSEVQKDTRDPQFDIRAIFYR 554
BLAST of Calpain-5 vs. Ensembl Cavefish
Match: ENSAMXT00000050823.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000063.1:117226:132090:1 gene:ENSAMXG00000038709.1 transcript:ENSAMXT00000050823.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 122.865 bits (307), Expect = 1.444e-28 Identity = 152/648 (23.46%), Postives = 260/648 (40.12%), Query Frame = 3
Query: 51 SQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDE-KVLSEHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKS-SPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTW-QKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRR----GPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
S + ++ +LK E L+ L+EDP F S LF + + W+RPG + + P L F SA + G++ W S L + +++ V+PN Q P Y G F F F G+ V++DDRLPT ++ L+ S +N EF AL+EKAYAK G Y L + A++DF+G + E + + S+ + ++N+ + I C I P + A H Y +TG + L + LL YF N V +IR+ + E G W + +++ + C + W + D+ C ++ S+++L K+ + + V F + P R G Q FL+N QY++ + + + + +++ + G N IG +F+VE NR YR+HD+ T+Q + + R + + L +GRY++IP G + L+V+++ + + C + + + R + F YP+ T++ V GA + + + + ISCE S + T PQ+D +FYR
Sbjct: 6 SAYKNQHYSELKRECLQQK---KLFEDPEFLCSNRSLFYRTPP---PGPVQWKRPG-MCEAPRL--FVEGISAHDLNQGEVGNCWFVAACSCLALKPQLWQKVIPNWKEQEWDPDRP-------DRYTGIFHFQFWIFGEWTDVVVDDRLPT-INGKLIYCHSKSNNEFWSALLEKAYAKLSGCYESLNGGNTGDAVVDFSGAVNEPVSLETGSFRTDSKTREKLFSDLLNVNDQGGIISCSIAASPHERELRLANGLVKGHAYSVTGVRRVRLG------HGLLAYFKND---TVDLIRMRNPWGKHEWRG--------AWSDRWGNVLRTFLCVFCRMSFDDW--CQNFTDVDVCRLM-----NTSVLSLQKR--WREVVHFGSWIKHSEPLRNRSGGCMNHRQTFLQNPQYILDLQDEDELLVSLQQRDMKIHRAHGQGE-----NLTIGFAIFKVELNRKYRMHDI-ITQQNVATSSYINARSVFLRKLLPKGRYIIIPSTFTPGILGDFMLRVYANQDLI--------------CRELDVDKPSVRCWSSFMG-------------------------------YPQVVTQVYVHGAEGLENQDSSGGADPYVIISCEKNSIQSEVQKDTRDPQFDIRAIFYR 559
BLAST of Calpain-5 vs. Ensembl Sea Lamprey
Match: capn1a (calpain 1, (mu/I) large subunit a [Source:ZFIN;Acc:ZDB-GENE-040426-1263]) HSP 1 Score: 82.4185 bits (202), Expect = 1.787e-16 Identity = 81/275 (29.45%), Postives = 122/275 (44.36%), Query Frame = 3
Query: 54 QMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFIS---NDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPY-----HVYLITGFTEI 851
+ S F ++K E L+ L+EDP+FPAS L + I+ N I W+RP +L + P + + A + G L WL + SL ++ VVP +F+++ Y G F F F + G+ V +DDRLPT L+ + S EF AL+EKAYAK G Y L + A+ DFTG +GE+ + + + I+ + K + C IE S + K +P H Y ITG ++
Sbjct: 24 KTKSKTFQEIKKECLQRK---ILFEDPDFPASLSSLHIRVQPTVITQSNNFIEWKRPWELVRHPEFI-VSGATRADINQGTLPDCWLLSAIASLTLYPDLLAKVVPM---GQSFHED----------YAGIFLFRFCQYGEWVDVHVDDRLPTK-KGELVFVQSGTGNEFWSALLEKAYAKLNGCYEALAYGFPVEALCDFTGGVGETFTMNNAPRDYWDGISR------AIKKGAIICCGIETLAASGR--GEKVTPMGLVEGHAYSITGVIKL 272
BLAST of Calpain-5 vs. Ensembl Sea Lamprey
Match: capn3a (calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]) HSP 1 Score: 73.9442 bits (180), Expect = 9.811e-14 Identity = 60/200 (30.00%), Postives = 93/200 (46.50%), Query Frame = 3
Query: 69 NFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFH--PGKLSPFWLPILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGE 662
+F +L++ L H L+EDP+FPA + ++ N W RP +L C+ + ++ H G L W+ I+ + EL+ + +F + +Y G F F F R G+ V++DDRLPT + L+ + S EF AL+EKAYAK G Y L+ + A+ DFTG + E
Sbjct: 52 SFEELRTLCLSHD---VLFEDPDFPAHNNSIYFSQNAPM---QFQWMRPKEL----CVPRSIVIEANHTHICEGDLGDCWVLAAIACMTMNE--ELLARVIPAGQSFEE----------NYAGIFHFQFWRFGEWVDVVVDDRLPT-FNNQLVFVQSAERNEFWSALLEKAYAKVHGSYEALKGGNTTEAMEDFTGGVTE 228
BLAST of Calpain-5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000152.1 (pep scaffold:Pmarinus_7.0:GL488881:291:5685:-1 gene:ENSPMAG00000000135.1 transcript:ENSPMAT00000000152.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 67.3958 bits (163), Expect = 8.267e-13 Identity = 36/81 (44.44%), Postives = 47/81 (58.02%), Query Frame = 3
Query: 420 SYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGE 662
+Y G F F F R G+ V++DDRLPT + L + S + EF AL+EKAYAK G Y L F A++DFTG + E
Sbjct: 34 AYAGIFHFKFWRYGEWVDVVVDDRLPT-LKGKLAFVRSCESNEFWGALLEKAYAKVCGSYEALRSGRFTEALVDFTGGVKE 113
BLAST of Calpain-5 vs. Ensembl Sea Lamprey
Match: capn9 (calpain 9 [Source:ZFIN;Acc:ZDB-GENE-010724-2]) HSP 1 Score: 63.1586 bits (152), Expect = 1.434e-10 Identity = 71/294 (24.15%), Postives = 112/294 (38.10%), Query Frame = 3
Query: 420 SYCGTFQFT--FRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIEL--PPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQL-------YWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLI 1265
Y G F F F G+ V IDDRLPT + L+ + S + EF AL+EKAY+K G Y L+ S A+ DFTG + + +D K + K K S C I++ +S ++S H Y ITG E+ N RG V ++R+ + + E NG + + W+ + + E W++ + + F + I L ++ YW D W + G + + F N Q+ +
Sbjct: 63 GYAGIFHFQQKFWHFGEWMDVFIDDRLPTK-NGQLVFVHSADKREFWSALLEKAYSKLNGSYEALKGGSIAEAMEDFTG----GVYELLDLKTAPPDLFRIMCKA--RQKGSMMGCSIDVTNASESEARTSNGLVKGHAYSITGVDEV-----------------NYRGQKVKLLRIRNPWGEVEWNGAWSDHAPE-WKALDQGERNRLQQVMAEDGEFWMSFDDFM---------RHFTKMEICNLTPDRVEREGDPRYWTVTVHDGRWLKGCTAGGCRNFPETFWTNPQFRL 322
BLAST of Calpain-5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003998.1 (pep scaffold:Pmarinus_7.0:GL501031:16584:50555:1 gene:ENSPMAG00000003440.1 transcript:ENSPMAT00000003998.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 60.077 bits (144), Expect = 1.770e-9 Identity = 35/79 (44.30%), Postives = 44/79 (55.70%), Query Frame = 3
Query: 414 SMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTG 650
S Y G F F F + G+ V+IDD LPT + L + S + EF AL+EKAYAK G Y L+ S A+ DFTG
Sbjct: 57 SDGYAGIFHFQFWQFGEWVDVVIDDLLPTQ-NNKLAFLHSTDRNEFWSALLEKAYAKLNGSYEALKGGSIAEAMEDFTG 134
BLAST of Calpain-5 vs. Ensembl Nematostella
Match: EDO34860 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN59]) HSP 1 Score: 110.153 bits (274), Expect = 6.374e-25 Identity = 154/618 (24.92%), Postives = 249/618 (40.29%), Query Frame = 3
Query: 198 IIWRRPGQLS--KLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLS-EHITSFYQKVVNMTKE-SFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLN--YFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLL-DISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQE-FLKNNQYLIMVP--QPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLP-NFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEVNIQLFCEN 2006
+ W+RP ++ +P L D S GKL W S L R + V+P+ Q ++P +Y G F+F F R G+ V++DD LPT V+ L+ I S EF ALIEKAYAK G Y L S A++DFTG + E+I S E ++ + KE S I++ ++ + + H Y +T ++ + +GL++ L N +FY +IRL + + E NG + W+K +KK G+ N E W+ + + + C+ L H+ N+ I + K+ W ++ W + G G V Q+ FLKN QY + + E + ++ + +K + + GN IG + +VE+NR YRVH + + F R ++ +L GRY+V+P + + + R FL+ D S K+ P CR P+ + V A ++ T L + + I CE + + TL P WD LFY S++ +Q++ N
Sbjct: 49 VEWKRPSEICHPAVPHLFVDGAD-SHDVTQGKLGNCWFVAACSSLALRPFLLYKVIPDKDKQEWDPKKP-------ENYQGIFRFRFYRQGEWTEVVVDDLLPT-VNGKLVYIHSKEKNEFWSALIEKAYAKLAGTYEALAAGSTADALVDFTGGVAEAISLSNGGYAEDQEKRLQLFKTLERGHKEKSLMSASIKINNRNEMEQRLDTGLIKGHAYGVTMVKKVTVG--EGLLS-LFNKKHFY--------MIRLRNPWGEKEWNGRWSDE-SEEWKKLKPGDRKKMGIVFENDGEFWMEFDDFCREFTDCT-LCHVINKAMIHKVFKQ---WHIYKHNYNWTQ-GQNAGGCVQNQDTFLKNPQYAFSITDNEGEDAMVSLMQEDRRK------DKSKGVGNLTIGFYIMKVEENRKYRVHSMFPK---AGDSIFINAREVMNRFFLAAGRYLVVPSTYEPHTPGHFMMRIFTRKRSNAMFLDQD-----------------------------------HSVGSKIWCCLP----RCRA--PQCVVSVYVTSAAGLQKTGKMSLTCDPYALIICEGRKIKTPIKYDTLIPSWDAGALFYVRQPQKSQIVVQIWDSN 590
BLAST of Calpain-5 vs. Ensembl Nematostella
Match: EDO48633 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ83]) HSP 1 Score: 85.8853 bits (211), Expect = 2.923e-17 Identity = 90/346 (26.01%), Postives = 147/346 (42.49%), Query Frame = 3
Query: 72 FLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFW-LPILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSS----PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS--LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLD 1088
F L+ +LL + L+ED +FPAS +LF++ S +IW+RP ++SK P + S+ G++ W + L L ++ V P +F EP SYCG F+F F + G+ +I+DD LPT + L S++ +EF AL+EKAYAK G Y + A+ D TG + E++ L + Y+ ++S C IE+ S+ + H Y ITG + L G V +IRL + + E G + W ++ K++ L ++ E W++ + L+
Sbjct: 12 FASLQRQLL---NSKRLFEDRDFPASNRVLFTRRR----SVQLIWKRPHEISKSPQFIA-RGFSSSDVAQGRIRNCWFVAALADLTTNIDLLHHVCPP---DQSF--EP-------GSYCGMFRFNFWQFGEWVEIIVDDYLPT-YNGRLYFTKSDDPDEFWSALLEKAYAKLCGSYEAMSLGRISEALQDMTGGMIENVDLQNPPHGLFNVMMKAYE------RQSLMGCSIEVKKYSSDQYEGTLENGLITRHAYSITGLRKATLSSLRGRPE-------------VDLIRLRNPHANSSEWRGAWSDRSPE-WNMLSSQEKRELNLTFDDDGEFWMSFDDFLN 316
BLAST of Calpain-5 vs. Ensembl Nematostella
Match: EDO40193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7W7]) HSP 1 Score: 76.6406 bits (187), Expect = 1.994e-14 Identity = 91/387 (23.51%), Postives = 152/387 (39.28%), Query Frame = 3
Query: 123 YEDPNFPA-SYDILFSK-PNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPY---HVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLI 1265
+ED F A I FSK P + F W+RP + + P + G L WL I+ L + + + VVP + ++ P +Y G +F + G+ + VIIDDRLPT + L+ + S+ EF AL+EKAYAK G Y L+ A+ DFTG L ES L++++ ++ ++ + + ++N+ + ++ H Y +TG ++ G ++ L N G+ E WQ + KK GL + E W++ + D K + + A+ + ++ S W R G + + F KN Q+ I
Sbjct: 20 FEDDEFLAIDSSIFFSKRPPRPFE-----WKRPSDICEDPHMF-VGGASRLDIQQGMLGDCWLLAAIAALTQHSALMNKVVP----------QDYVANPNDRNYTGALKFCIWQYGEWKDVIIDDRLPT-YNGKLVFVHSSERNEFWSALLEKAYAKLNGSYESLKGGQTGEALEDFTGGLAESFD-------LNKNVPKDLFCIMQTAEKRHALMGCSIAAKANEIEAKLNNGLVKGHAYTVTGCRQVKAKTRRGDLDVELVRVRNPWGNEREWKGAWGDGSSE------------WQLLSDYEKKSIGLTFEDDGEFWMSFD---DFKKHFTTLEICMLGADSAMEGSSVGFESRSEHGMWRRGLTAGGCRNFIKTFHKNPQFRI 367
BLAST of Calpain-5 vs. Ensembl Nematostella
Match: EDO48536 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ84]) HSP 1 Score: 55.4546 bits (132), Expect = 7.402e-8 Identity = 47/142 (33.10%), Postives = 67/142 (47.18%), Query Frame = 3
Query: 246 KFNNDQSARF--HPGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGE 662
+F D ++R G L WL +S+L + + + ++P + + + F S Y G F + G+ VIIDDRLPT L+ + S EF AL+EKAYAK G Y L+ A+ DFTG L E
Sbjct: 18 RFYVDGASRLDIKQGVLGNCWLLAAMSVLAQHKPLVDRIMP----EGVYEADRF-----SPRYTGKVVFKLWQYGEWVTVIIDDRLPT-YKGKLVFVHSPEKNEFWCALLEKAYAKIHGSYEALKGGLTGEALEDFTGGLSE 149
BLAST of Calpain-5 vs. Ensembl Medaka
Match: ENSORLT00000002598.2 (calpain-5 [Source:NCBI gene;Acc:101166153]) HSP 1 Score: 156.377 bits (394), Expect = 1.831e-39 Identity = 167/632 (26.42%), Postives = 269/632 (42.56%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKERN-MFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI------CKSVDEKVLSEHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHR-DGPRR----GPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQP-DICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
L+EDP FP S LF + N+ ++W+RPG+LS P L F + SA H G+L W S L R +++ V+P+ Q ++P SY G F F F R G+ V+IDD LPT VS LL SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E I K DEK +++V+ + I C I ++ ++ H Y +T + L + LL +F + + S+ IR+ + E NG + W+K + ++K G+ ++ E W+T + L +L + N S ++L K W+ V W R D P R G +N QY+ V +PE V+ +Q+ N IG ++ +VE NR+YR+H +Q + + R + + L++GRYV++P AG ++ L++F+ PS+ R+++ +P C YP T++ V A + + ++ + + I CE + S++ +T SP +D LFYR
Sbjct: 23 LFEDPLFPPSDKSLFYEGNR---VGQVVWKRPGELSSDPHL--FVDGISAHDLHQGQLGNCWFVAACSSLASREALWQKVIPDWKEQEWDKEKP-------QSYAGIFHFRFWRFGEWVDVVIDDLLPT-VSGQLLYGHSNDHNEFWSALVEKAYAKMFGCYEALDGGNTADALVDFTGGVSEPIDLIQNGYKEDDEKR-----DELFERVLKVHNRGGLISCSIRATSAADMEARLGCGLVKGHAYAVTDVRRVRLG------HGLLAFFKSDKLSM---IRMRNPWGQKEWNGPWSD-SSEEWKKVSKSEREKMGVTVDDDGEFWMTFDDFLTNFTDLILCRLIN-TSYLSLHKT---WEEVVVRGSWRRHDEPLRNRAGGCSNNKHSHFQNPQYVFDVTKPE----DEVMVCLQQKDRRATLKEGRGENLPIGFDIQKVELNRVYRMH---AAQQKVGGSIYINSRAVFLRIDLKKGRYVIVPTTFEAGLEGEFLLRIFTD----------------------------------------------VPSNCRELTLDEPRRSCWSGLRGYPSLVTQVYVQQANGLAGQDSDRVSDPYVIIRCEGQEVRSDVHKNTCSPVFDTKALFYR 569
BLAST of Calpain-5 vs. Ensembl Medaka
Match: capn5b (calpain 5 [Source:NCBI gene;Acc:101155135]) HSP 1 Score: 145.206 bits (365), Expect = 1.537e-35 Identity = 156/625 (24.96%), Postives = 258/625 (41.28%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHI-TSFYQKVVNM-TKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW-HRDGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
L+EDP FPA D LF N+ ++W+RP +L + P L F + SA H G+L W S L R ++++ V+P+ Q ++P SY G F F F R G+ V+IDDRLPT V L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + + E +++V+ + ++ C I + ++ H Y +T ++ L + LL +F + + + +IR+ + +E + W K + ++K G+ + E W+T + +L + N S +++ K W+ W HR P R I + FL+N QY+ V + E VL +Q+ N IG ++ VE NR YR+H +Q + + R + + ++ GRYV+IP G ++ L+VF+ D+P C + + P C YP+ T++ V+ A ++ + + + I+CE E S + T P +D LFYR
Sbjct: 98 LFEDPRFPAIDDSLFYLGNR---IGRVVWKRPRELCEDPHL--FVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDTEKP-------DSYTGIFHFRFWRFGEWVDVVIDDRLPT-VDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPVDLLEGQMATDEAARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LQMIRMRNPWGEKEWSGPWSDSSEEWNKVSKSEREKLGVTVQDDGEFWMTFDDFCQYFTDLILCRLIN-TSYLSIHKT---WEEEVIRGLWVHRQDPLRNRAGGCINHKSTFLQNPQYVFDVKKLE----DEVLICLQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHT---AQQKVAGSIYINSRCVFLRKEMKEGRYVIIPTTFDPGQQGEFLLRVFT------------DVPSD--CKELTLDEP-------------------------------PQTCWTGMCGYPQLVTQVHVLNAEGLQGQDSNGAVDSYVVITCEGERVRSPVQKDTRCPNFDFKGLFYR 644
BLAST of Calpain-5 vs. Ensembl Medaka
Match: capn5b (calpain 5 [Source:NCBI gene;Acc:101155135]) HSP 1 Score: 141.739 bits (356), Expect = 1.948e-34 Identity = 159/642 (24.77%), Postives = 259/642 (40.34%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSAR-FHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHI-TSFYQKVVNM-TKESFGICQIELPPQSNKKSSAKSS--PYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFW-HRDGP--RRGPKVIGQE--FLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDI-----------------RLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
L+EDP FPA D LF N+ ++W+RP +L + P L F + SA H G+L W S L R ++++ V+P+ Q ++P SY G F F F R G+ V+IDDRLPT V L+ SN++ EF AL+EKAYAK G Y L+ + A++DFTG + E + + E +++V+ + ++ C I + ++ H Y +T ++ L + LL +F + + + +IR+ + +E + W K + ++K G+ + E W+T + +L + N S +++ K W+ W HR P R I + FL+N QY+ V + E VL +Q+ N IG ++ VE NR YR+H +Q + + R + + ++ GRYV+IP G ++ L+VF+ D+P C + + P C YP+ T++ V+ A + RL Q + + I+CE E S + T P +D LFYR
Sbjct: 98 LFEDPRFPAIDDSLFYLGNR---IGRVVWKRPRELCEDPHL--FVDGISAHDLHQGQLGNCWFVAACSSLASRESLWQKVIPDWKEQEWDTEKP-------DSYTGIFHFRFWRFGEWVDVVIDDRLPT-VDNQLVYCHSNDSNEFWSALVEKAYAKVYGCYEALDGGNTADALVDFTGGVSEPVDLLEGQMATDEAARNQLFERVLKVHNRDGLISCSIRATTVEDMEARLDCGLVKGHAYAVTDVRKVRLG------HGLLAFFKSEK---LQMIRMRNPWGEKEWSGPWSDSSEEWNKVSKSEREKLGVTVQDDGEFWMTFDDFCQYFTDLILCRLIN-TSYLSIHKT---WEEEVIRGLWVHRQDPLRNRAGGCINHKSTFLQNPQYVFDVKKLE----DEVLICLQQKERRATPKEGKGENLAIGFDIHRVELNRKYRMHT---AQQKVAGSIYINSRCVFLRKEMKEGRYVIIPTTFDPGQQGEFLLRVFT------------DVPSD--CKELTLDEP-------------------------------PQTCWTGMCGYPQLVTQVHVLNAEGLQGQDSNGELHCLNPQKKRLLGKAQAVDSYVVITCEGERVRSPVQKDTRCPNFDFKGLFYR 661
BLAST of Calpain-5 vs. Ensembl Medaka
Match: ENSORLT00000034978.1 (calpain-5 [Source:NCBI gene;Acc:101158220]) HSP 1 Score: 137.117 bits (344), Expect = 4.048e-33 Identity = 167/659 (25.34%), Postives = 271/659 (41.12%), Query Frame = 3
Query: 33 MSDAVESQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVL---SEHITSFYQKVVNMTKESFGI--CQIELPPQSNK-KSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVE----KLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGP----RRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
M +VE+ + ++ LK + L++ L+EDP FPA+ +F + E + W+RPG++S P L S + G + W S L + ++++ V+P+ Q + P +Y G F F F G+ V++DDRLPT ++ L+ SN+ EF AL+EKAYAK G Y L+ + A++DF+G + E I ++ E E + ++ ++ + E GI C I+ P + K + H Y +T I L + L+ YF N +P+IR+ + E NG + K + GL + E W++ E K D CS++ S++++ K W V W + G R G FL+N QY+ V T I+ VL +Q+ N IG + +VE NR YR+H L K + F R + + L +GRY++IP AG ++ L++F+ + E D P+ + TP L+ YP+ T + V A + + + ISCE + S + TL+PQ+D +FYR
Sbjct: 1 MFSSVEA-FKNQHYADLKRDCLQNK---KLFEDPEFPANNSSVFYR---EPPPGTVQWKRPGEISSDPHLF-VEGINSHDLNQGNVGNCWFVAASSCLALKPHLWKKVIPDWQEQEWDPKHP-------ENYAGIFHFQFWVFGQWTDVVVDDRLPT-INGELIYCQSNSKNEFWSALLEKAYAKLYGCYESLKGGNTGDAVVDFSGAVYEPI--NLQEGAFYKDPEKQNTLFENLLKVY-ERGGIISCSIKAEPHELEFKMANGLVKGHAYSVTAVKRIRLG------HGLVAYFKN---ETIPMIRMRNPWGRMEWNGSWSDSSEEW-SKIGDTERHNLGLTVEDDGEFWMSFEDWCKKFTDADVCSII-----NTSLVSIHKT---WSEVLHFGSWTKHGDPLLNRCGGCGNNNTFLQNPQYMFDV----TKEIEEVLITLQQKDRRTFKKTGQGENLSIGFSVLKVELNRKYRIHRLLSQKSMESSV-FINARSVFVRCILPQGRYIIIPSTFQAGELGEFMLRIFTDVDSGCRELTE-DKPKVKCWTP----------------LFG----------------------------YPQAVTHVFVEKAERLENQDRTAGADPYVIISCEGKSVKSIVHKDTLNPQFDTSAVFYR 572
BLAST of Calpain-5 vs. Ensembl Medaka
Match: ENSORLT00000039414.1 (calpain-5 [Source:NCBI gene;Acc:101158220]) HSP 1 Score: 134.035 bits (336), Expect = 3.329e-32 Identity = 158/627 (25.20%), Postives = 250/627 (39.87%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKER-NMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGI--CQIELPPQSNK-KSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVE----KLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGP----RRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP-WIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYR 1958
L+ DP FPA LF K E + W+RP ++S P L S + G++ W S L ++++ V+P+ Q + P +Y G F F F G+ V++DDRLPT ++ L+ SN+ +EF AL+EKAYAK G Y L A++DFTG + E I + + + + + E GI C I+ P + K + H Y +T I L ++L+ +F + +P+IR+ + E NG + W K + GL + E W++ E K D CS++ S++++ K W V W + G R G FL+N QY+ V + I+ VL +Q+ N IG + +VE NR YR+H L K + F R + + L +GRY++IP AG ++ L++F+ + E D P+ + TP L+ YP+ T + V A + + + ISCE + S + TL+PQ+D +FYR
Sbjct: 26 LFRDPEFPADDSSLFYK---EPPPGKVEWKRPWKISSDPHLF-VEGINSHDLYQGEIGNCWFVAASSCLAMMPHLWQKVIPDWQEQEWDPKHP-------ENYAGIFHFQFWVFGQWTDVVVDDRLPT-INGKLIYCHSNSKKEFWSALLEKAYAKLYGCYESLSGGFAGDAVVDFTGAVYERIDLEREAFYKDQRNQDYLFENLLKVYEHGGIISCSIKAKPDELEFKMANGLVKGHAYSVTAVKRI------HLCHELVAHF---KTETLPMIRMRNPWGKMEWNGAWSD-SSEEWSKIGDTERHNLGLTVEDDGEFWMSFEDWCKKFTDADVCSII-----NTSLVSIHKT---WSEVLHFGSWTKHGDPLLNRCGGCGNNNTFLQNPQYMFDVTKE----IEEVLITLQQKDRRTFKKTGQGENLSIGFSVLKVELNRKYRIHRLLSQKSMESSV-FINARSVFVRCILPQGRYIIIPSTFQAGELGEFMLRIFTDVDSGCRELTE-DKPKVKCWTP----------------LFG----------------------------YPQAVTHVFVEKAERLENQDRTAGADPYVIISCEGKSVKSIVHKDTLNPQFDTSAVFYR 572
BLAST of Calpain-5 vs. Planmine SMEST
Match: SMESG000012000.1 (SMESG000012000.1) HSP 1 Score: 1442.17 bits (3732), Expect = 0.000e+0 Identity = 714/716 (99.72%), Postives = 715/716 (99.86%), Query Frame = 3
Query: 33 MSDAVESQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEVNIQLFCENKLGLKSRIFDYTFSEKELRDKCRVSLPIQDDXXXXXXXXXXXXXXXXXXYFLSEFNRA 2180
MSDAVESQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKS PYHVY+ITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEVNIQLFCENKLGLKSRIFDYTFSEKELRDKCRVSLPIQDDISPENESEENRIIIEIENYFLSEFNRA
Sbjct: 1 MSDAVESQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFHPGKLSPFWLPILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGICQIELPPQSNKKSSAKSPPYHVYIITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLPNFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEVNIQLFCENKLGLKSRIFDYTFSEKELRDKCRVSLPIQDDISPENESEENRIIIEIENYFLSEFNRA 716
BLAST of Calpain-5 vs. Planmine SMEST
Match: SMESG000078391.1 (SMESG000078391.1) HSP 1 Score: 242.276 bits (617), Expect = 4.316e-69 Identity = 192/657 (29.22%), Postives = 312/657 (47.49%), Query Frame = 3
Query: 195 DIIWRRPGQLSKLPCLMK-FNNDQSARFHPGKLSPFWLPILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCH-LLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESI--------CKSVDEKVLSEHITSFYQKVV-----NMTKESFGICQIELPPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMSLSDCEENGIID-KYVVKT--WQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAKKQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLT--NVQKYVEVGANAIQTAGNTQIGIELFEVEKNRLYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIPWIAAGSGTKYTLQVFSSDNRVETTFLEFDIPEQQLCTPCQIKQTAYRTQNRFKSLYDRSTSEYTPSSQRKVSTIQPDICQECREYYPKRATRISVIGATDIRLTATYQLP----NFFFKISCENELYISNLVSSTLSPQWDEHFLFYRINNDFSEVNIQLFCENKLGLKSRIFDYTFSEKEL-RDKCRVSLPI 2090
+IIW+RP L PCL++ ++ + G +P W PI+I+LLK +N+ LVV ++ Q+ FN+ SY G F+F+F ++IDD+LP + L S+N EEFLP+L+EKAYA+FLGGY L+ FL +M+FTG + ES+ ++K+L + + S V+ N +E + + P +N A H YLI+GFTEIPL LV +++ F + I L+ L + NG + + V + W+ V++ + L+ N+ E WIT++ + K +L H+ + + + K W + + P K + EFLKN+QYLI V +K VLT N+ K + +T + L +VE NRLYR+HDL +++ + + G ++ L +G+Y++IPW S +Y L+++SS + + L + P C C + T NR + L + S + +P+ + C C P+ A RI+++ A + L N +F I N Y SN + PQW+E F+ YR +++I+LF E ++G + FSE +L +D+ L I
Sbjct: 41 EIIWKRPIDLVDDPCLLETYDQEIDGYIICGSQTPAWFPIVITLLKTKNILHLVVKDIKKQDNFNKS---------SYIGCFEFSFFGKNMWTTILIDDKLPHIAGENKSLYCVSSNKEEFLPSLLEKAYARFLGGYGKLQTVDFLDVLMNFTGGIVESVDLKRNISLLTYQEQKILFKKLRSSVSDVMVIGLTNSNQEE----KTDFPEDNNNFVLAHD---HYYLISGFTEIPLQSNLNLVGTVISSFLDIHNH--ECINLLRLHNPVLNGDWNGAFSVGSPEWEGINESVRRVFNLKFNHEFEFWITMKDFIRNMKNIILCHIPSYSYVDRFLKGVKVWSSFHHNGIYSSLIPGGSIKSLDDEFLKNDQYLIEVYNQNLIEMKFVLTMNNIDKSLRY---------DTGCCLTLLKVESNRLYRLHDLQNSEISNLRLLSESGSMYSVSTTLCKGKYILIPWRKEISEVEYYLRIYSSQKSINISLLLLNQPPFVQCLKCSLVNTIL---NRKEFLNNVSGNCESPN-----DSFTKHRCSSCFSVNPQSAVRITIVSAYGLESNEDNILNTRSVNSYFVIKYPNFTYRSNTKYLSNKPQWNESFIVYRNAKQRDDISIELFAEQRIGFDKSFGIHYFSETDLCKDEIVAGLRI 662
BLAST of Calpain-5 vs. Planmine SMEST
Match: SMESG000017238.1 (SMESG000017238.1) HSP 1 Score: 111.309 bits (277), Expect = 7.494e-25 Identity = 122/482 (25.31%), Postives = 203/482 (42.12%), Query Frame = 3
Query: 120 LYEDPNFPASYDILFSKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARFH--PGKLSPFWLPILISLLKERNM-FELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVV-NMTKESFGICQIELPPQ-SNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLLNYFYNSRGSLVPVIRLMS-LSDCEENGIIDKYVVKTWQKCTTLVKKKYGLECNNGKEHWITVEKLLDISKCSVLFHMFNRNSIIALAK--KQLYWKYVSFDHFWHRDGPRRGPKVIGQEFLKNNQYLIMVPQPETCCIKMVLTNVQKYVEVGANAIQTAGNT--QIGIELFEVEKNR---LYRVHDLNHTKQVTCQTDFQQGRYIVITAYLERGRYVVIP 1526
LYED +FPA+ + ++ NK + W+RP ++ P +F ++RF G+L WL ++ L + M F V PN + F YCG F F F G+ + +IIDDRLPT + L+ + S ++ EF AL+EKAYAK G Y L+ S A+ DFTG + E I D + +E +Q ++ + +K S C I+ P+ + K H Y IT +I P YN G ++ ++R+ + E NG +W + + GL +N E W++ ++ + + H+ ++ +L K + W+ W + G + F N Q+ I+V P+ + T + ++ +I+ GN IG L+++ L +V ++V F R + L G Y +IP
Sbjct: 105 LYEDEDFPANGESIYFTSNK---GGNFQWKRPHEICSDP---QFFVGGASRFDVCQGELGDCWLLAAVASLSMQTMLFSQVAPN--------DQSF----AKGKYCGAFHFNFWHYGEWKEIIIDDRLPTR-NNRLVFMHSRDSNEFWSALLEKAYAKLCGSYELLKGGSSSEAMEDFTGGITEIIDLKGDNRKNNESKEKLFQIMIQSHSKMSLMGCSIDANPKVTEAKLGNGLIMGHAYSITAVKQISAKVPG----------YN--GDIIKLVRIRNPWGQSEWNGEWGDS-SDSWHHISDKERGVLGLNFDNDGEFWMSFNDFVNNFEKLEICHLGPQSVGESLLKNSNKRTWESSVQQGAWKKGASAGGCLNFQETFWSNPQFRIVVTDPDKDDTDNMGTVIIGLLQKNMRSIKNRGNDLFPIGYVLYKLPSTDCGPLDKVF-FRTNRKVAMSPAFINMREVCGRHRLPPGSYAIIP 553
BLAST of Calpain-5 vs. Planmine SMEST
Match: SMESG000062904.1 (SMESG000062904.1) HSP 1 Score: 102.834 bits (255), Expect = 2.650e-22 Identity = 93/274 (33.94%), Postives = 135/274 (49.27%), Query Frame = 3
Query: 87 SELLRHPDGVA-LYEDPNFPASYDILF-SKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARF--HPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLL 890
S++L H G L+EDP+FPA +F SK +K + + W+RP + K P L D +RF G L WL ++ SL +F+ VVP ++Q+ +Y G F+F F R GK V++DDRLPT L+ + S +N EF AL+EKAYAK G Y L S + A+ DFTG L E+I ++EK E Y +++ K+S + C IE ++ S H Y IT ++ DG V +L+
Sbjct: 53 SDILEHLKGTGKLWEDPDFPADTSSVFVSKASKGY--REFEWKRPKDICKNPQLFA---DGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVP--TDQD-----------FDKNYIGAFRFRFWRFGKWVEVVVDDRLPT-YRGRLVFMHSADNNEFWSALLEKAYAKMCGIYESLNGGSQIEAMEDFTGGLCETI--KLNEK---ERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISG-HAYSITDVQKVK--QTDGTVVELV 299
BLAST of Calpain-5 vs. Planmine SMEST
Match: SMESG000062904.1 (SMESG000062904.1) HSP 1 Score: 102.834 bits (255), Expect = 2.783e-22 Identity = 93/274 (33.94%), Postives = 135/274 (49.27%), Query Frame = 3
Query: 87 SELLRHPDGVA-LYEDPNFPASYDILF-SKPNKEFISNDIIWRRPGQLSKLPCLMKFNNDQSARF--HPGKLSPFWL-PILISLLKERNMFELVVPNLSNQNTFNQEPFIITPVSMSYCGTFQFTFRRGGKLEHVIIDDRLPTTVSCHLLSISSNNNEEFLPALIEKAYAKFLGGYYCLEKESFLTAIMDFTGVLGESICKSVDEKVLSEHITSFYQKVVNMTKESFGI-CQIELPPQSNKKSSAKSSPYHVYLITGFTEIPLDPPDGLVNDLL 890
S++L H G L+EDP+FPA +F SK +K + + W+RP + K P L D +RF G L WL ++ SL +F+ VVP ++Q+ +Y G F+F F R GK V++DDRLPT L+ + S +N EF AL+EKAYAK G Y L S + A+ DFTG L E+I ++EK E Y +++ K+S + C IE ++ S H Y IT ++ DG V +L+
Sbjct: 53 SDILEHLKGTGKLWEDPDFPADTSSVFVSKASKGY--REFEWKRPKDICKNPQLFA---DGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVP--TDQD-----------FDKNYIGAFRFRFWRFGKWVEVVVDDRLPT-YRGRLVFMHSADNNEFWSALLEKAYAKMCGIYESLNGGSQIEAMEDFTGGLCETI--KLNEK---ERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISG-HAYSITDVQKVK--QTDGTVVELV 299 The following BLAST results are available for this feature:
BLAST of Calpain-5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Calpain-5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Calpain-5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Calpain-5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Calpain-5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Calpain-5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Calpain-5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Calpain-5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Calpain-5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Calpain-5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Calpain-5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Calpain-5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 4
BLAST of Calpain-5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Calpain-5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30017727 ID=SMED30017727|Name=Calpain-5|organism=Schmidtea mediterranea sexual|type=transcript|length=2463bpback to top protein sequence of SMED30017727-orf-1 >SMED30017727-orf-1 ID=SMED30017727-orf-1|Name=SMED30017727-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=717bp MSDAVESQMSSPNFLKLKSELLRHPDGVALYEDPNFPASYDILFSKPNKEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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