SMED30017611

Overview
NameSMED30017611
Smed IDSMED30017611
Length (bp)1930
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30017611 (SMED30017611) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017611 (SMED30017611) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017611 (SMED30017611) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017611 (SMED30017611) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Alignments
SMED30017611 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000552:27365..39245 +9369
Homology
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BLAST of SMED30017611 vs. Planmine SMEST
Match: SMESG000038056.1 (SMESG000038056.1)

HSP 1 Score: 1055.43 bits (2728), Expect = 0.000e+0
Identity = 530/533 (99.44%), Postives = 532/533 (99.81%), Query Frame = 3 
Query:  123 MDDQCSVLSSCYWDATSVTSDGTIPTEFYHTPTGSINDIPLSEISGTSRDSRNFTPTFSNMNEPKFIDEDLELVKSEIEKAPSIELAPIASEVVLRPPLIKQQKAEIEENPNNKNEDLSRKRSWKHDIAKRFSSTRSRFIKSFXXXXXXXXXXXXXXXXNFFQSMRHSIRKIKSFDKKSNRNKSVEKVPSKSASAVIGSDCDYFNFAKDTEGSELTKQSEEISIDLELEGGTPLKPRRQFTQEKYLTGSRDVLKKAFNSLKRKSHMQDDKSLFDICSTVSYLFSNELEEMSFAKSEEKMFQKFMTNCVNSFLDVHKIQIGTSLEMINRNIGEHVDLYSPNLDVINKSKKYQITEIFGLKGTCIITNTGNKKKFIALKQGVKNWVLFTSFLQHIEQDTPLPIHHQEWICFPAQSITNLYIEKNFFNPLHVECKLLLDSLIQLIVILYCQNIHNIHIKLNPSNILIVSSNLSVIFVFIESDAGCASTDESQWLNLYDYSDKEMRLAYQQSVMKLNKYVEKFLSLPENASHLITAS 1721
            MDDQCSVLSSCYWDATSVTSDGTIPTEFYHTPTGSINDIPLSEISGTSRDSRNFTPTFSNMNEPKFIDEDLELVKSEIEKAPSIELAPIASEVVLRPPLIKQQKAEIEENPNNKNEDLSRKRSWKHDIAKRFSSTRSRFIKSFSISKTKDESKKEESSKNFFQSMRHSIRKIKSFDKKSNRNKSVEKVPSKSASAVIGSDCDYFNFAKDTE SELTKQSEEISIDLELEGGTPLKPRRQFTQEKYLTGSRDVLKKAFNSLKRKSHMQDDKSLFDICSTVSYLFSNELEEMSFAKS+EKMFQKFMTNCVNSFLDVHKIQIGTSLEMINRNIGEHVDLYSPNLDVINKSKKYQITEIFGLKGTCIITNTGNKKKFIALKQGVKNWVLFTSFLQHIEQDTPLPIHHQEWICFPAQSITNLYIEKNFFNPLHVECKLLLDSLIQLIVILYCQNIHNIHIKLNPSNILIVSSNLSVIFVFIESDAGCASTDESQWLNLYDYSDKEMRL+YQQSVMKLNKYVEKFLSLPENASHLITAS
Sbjct:    1 MDDQCSVLSSCYWDATSVTSDGTIPTEFYHTPTGSINDIPLSEISGTSRDSRNFTPTFSNMNEPKFIDEDLELVKSEIEKAPSIELAPIASEVVLRPPLIKQQKAEIEENPNNKNEDLSRKRSWKHDIAKRFSSTRSRFIKSFSISKTKDESKKEESSKNFFQSMRHSIRKIKSFDKKSNRNKSVEKVPSKSASAVIGSDCDYFNFAKDTEDSELTKQSEEISIDLELEGGTPLKPRRQFTQEKYLTGSRDVLKKAFNSLKRKSHMQDDKSLFDICSTVSYLFSNELEEMSFAKSDEKMFQKFMTNCVNSFLDVHKIQIGTSLEMINRNIGEHVDLYSPNLDVINKSKKYQITEIFGLKGTCIITNTGNKKKFIALKQGVKNWVLFTSFLQHIEQDTPLPIHHQEWICFPAQSITNLYIEKNFFNPLHVECKLLLDSLIQLIVILYCQNIHNIHIKLNPSNILIVSSNLSVIFVFIESDAGCASTDESQWLNLYDYSDKEMRLSYQQSVMKLNKYVEKFLSLPENASHLITAS 533
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BLAST of SMED30017611 vs. Planmine SMEST
Match: SMESG000061850.1 (SMESG000061850.1)

HSP 1 Score: 63.929 bits (154), Expect = 1.694e-10
Identity = 68/263 (25.86%), Postives = 118/263 (44.87%), Query Frame = 3 
Query:  939 FDICSTVSYLFSNELEEMSFAKSEEKMFQKFMTNCVNSFLDVHKIQIGTSLEMINRNIGEHVDLYSPNLDVINKSKKYQITEIFGLKGTCIITNTGNKKKFIALKQG-VKNWVLFTSFLQHIEQDTPLPIHHQEWICFPAQSITNLYIEKNFFNPLHVECKLLLDSLIQLIVILYCQNIHNIHIKLNPSNILIVSSNLSVIFVFIESDAGCASTDESQWLNLYDYSDKE-MRLAYQQSVMKLNKYVEKFLSLPENASHLITAS 1721
            FDI   ++   ++EL + + +      F +++ +C+ +F+    +Q+  +L  +N  +   +DL S +L   N+ + Y +  I G K    I +   K+K+I+LK    ++WV   +      + TP     Q WICFP  SI     E +       +  LL   LI LI I    N       ++P+ IL+VSS   V+ +F E    C +  E   L  +  +  E +     +S     K ++ F   PE  S LI+++
Sbjct:  180 FDIIPLLNNYLASELRDSAISD-----FDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISL-TSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINA-----SKTTP----GQRWICFPIHSINTFENEISLSEKDDFQKTLL--KLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKE----CLNESELFSLEFFGCNSIENISEVLLESKRNTQKLIKDFFQRPEVISILISST 421
[Close]
BLAST of SMED30017611 vs. Planmine SMEST
Match: SMESG000061850.1 (SMESG000061850.1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.409e-10
Identity = 68/263 (25.86%), Postives = 118/263 (44.87%), Query Frame = 3 
Query:  939 FDICSTVSYLFSNELEEMSFAKSEEKMFQKFMTNCVNSFLDVHKIQIGTSLEMINRNIGEHVDLYSPNLDVINKSKKYQITEIFGLKGTCIITNTGNKKKFIALKQG-VKNWVLFTSFLQHIEQDTPLPIHHQEWICFPAQSITNLYIEKNFFNPLHVECKLLLDSLIQLIVILYCQNIHNIHIKLNPSNILIVSSNLSVIFVFIESDAGCASTDESQWLNLYDYSDKE-MRLAYQQSVMKLNKYVEKFLSLPENASHLITAS 1721
            FDI   ++   ++EL + + +      F +++ +C+ +F+    +Q+  +L  +N  +   +DL S +L   N+ + Y +  I G K    I +   K+K+I+LK    ++WV   +      + TP     Q WICFP  SI     E +       +  LL   LI LI I    N       ++P+ IL+VSS   V+ +F E    C +  E   L  +  +  E +     +S     K ++ F   PE  S LI+++
Sbjct:  203 FDIIPLLNNYLASELRDSAISD-----FDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISL-TSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINA-----SKTTP----GQRWICFPIHSINTFENEISLSEKDDFQKTLL--KLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKE----CLNESELFSLEFFGCNSIENISEVLLESKRNTQKLIKDFFQRPEVISILISST 444
The following BLAST results are available for this feature:
BLAST of SMED30017611 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017611 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000038056.10.000e+099.44SMESG000038056.1[more]
SMESG000061850.11.694e-1025.86SMESG000061850.1[more]
SMESG000061850.12.409e-1025.86SMESG000061850.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017611 ID=SMED30017611|Name=SMED30017611|organism=Schmidtea mediterranea sexual|type=transcript|length=1930bp
TTCAAATTGCAGATTTCAGTGGTTGTTGTTGTTGTTTTCGTTGTTGTAGT
ATTATTATTATTGTTAACTTTGTGTTATAATAAATCGATATCAGAATAAA
TAAATTCTCTTTACACATTTGAATGGATGATCAATGTTCGGTGCTTAGCA
GTTGCTACTGGGACGCTACTAGTGTGACTAGTGATGGAACTATTCCGACA
GAATTCTATCATACACCGACTGGTTCAATAAACGACATTCCATTATCTGA
AATTAGCGGCACCAGCAGAGATTCAAGAAACTTTACACCGACATTTTCCA
ATATGAATGAACCGAAATTTATTGACGAAGATTTGGAATTAGTGAAAAGT
GAAATTGAGAAAGCTCCATCAATTGAATTGGCACCGATTGCCAGTGAAGT
CGTCTTGAGGCCGCCATTAATCAAACAACAGAAAGCGGAAATTGAAGAAA
ATCCCAACAATAAAAATGAGGATTTGTCTAGAAAGCGAAGTTGGAAGCAT
GATATTGCCAAGAGATTCTCGTCAACTAGGTCGAGGTTTATTAAATCTTT
TTCTATTAGTAAAACGAAAGATGAATCTAAAAAGGAAGAATCGTCAAAAA
ACTTTTTCCAAAGTATGCGGCATTCAATACGAAAAATCAAATCGTTTGAT
AAGAAATCCAACCGAAACAAAAGCGTGGAGAAAGTTCCGTCGAAATCGGC
ATCCGCTGTCATTGGATCGGATTGCGATTATTTTAACTTCGCCAAGGACA
CGGAAGGTTCTGAATTAACGAAGCAAAGTGAAGAAATCTCAATTGATCTA
GAGCTTGAAGGAGGTACGCCACTCAAGCCACGAAGACAGTTTACACAGGA
GAAATACTTGACTGGCTCTAGAGATGTATTGAAGAAAGCTTTCAATTCCT
TGAAACGAAAATCCCACATGCAGGATGATAAATCCCTATTCGACATTTGT
TCGACAGTTTCTTATCTATTCTCCAATGAGCTCGAAGAAATGTCGTTTGC
CAAGAGTGAAGAGAAAATGTTTCAAAAGTTTATGACGAATTGCGTCAACT
CGTTTCTGGATGTCCACAAGATTCAGATTGGGACATCTCTGGAAATGATC
AACCGAAACATTGGCGAACATGTCGATTTGTACTCGCCGAATTTGGATGT
CATTAATAAATCTAAAAAATATCAAATCACAGAAATATTCGGACTAAAAG
GGACATGTATCATTACCAATACTGGGAATAAGAAAAAGTTTATTGCCCTG
AAACAAGGGGTCAAAAATTGGGTTTTGTTCACCAGTTTCTTGCAGCATAT
TGAACAGGACACTCCGCTTCCTATTCATCATCAAGAATGGATCTGTTTTC
CCGCTCAGTCAATCACCAACTTATACATTGAAAAGAATTTTTTCAATCCT
CTCCATGTTGAATGCAAATTACTTTTAGACAGTCTAATTCAGCTGATTGT
CATTTTGTATTGCCAAAATATCCACAACATACATATCAAATTGAATCCCT
CTAACATTCTGATTGTCTCCTCTAATCTTTCGGTCATTTTTGTATTCATC
GAATCAGATGCCGGATGCGCTAGTACCGATGAGAGCCAATGGCTGAACTT
GTACGATTACAGCGATAAAGAAATGAGACTAGCATATCAACAATCTGTCA
TGAAACTCAACAAATACGTCGAGAAATTCCTCTCTTTACCGGAGAATGCT
TCTCATTTGATTACAGCTTCATATCCTCTTGATTATTTGTTTTATCTTTT
ATTGAAATTGAACAAAGATATTAATATTCTTTTAAACAGCTTATAAATTT
TTATTTTATGAAAATTCTGATTTTTCATTATTTTACACATTTGGTAAGTT
TTTTCTTTAACTCGAATAAGATTTGATATAATTGCCTTCAATTTATTATG
TAATATTGTTCAATAAATCTGTGAGAATTC
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protein sequence of SMED30017611-orf-1

>SMED30017611-orf-1 ID=SMED30017611-orf-1|Name=SMED30017611-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=558bp
MDDQCSVLSSCYWDATSVTSDGTIPTEFYHTPTGSINDIPLSEISGTSRD
SRNFTPTFSNMNEPKFIDEDLELVKSEIEKAPSIELAPIASEVVLRPPLI
KQQKAEIEENPNNKNEDLSRKRSWKHDIAKRFSSTRSRFIKSFSISKTKD
ESKKEESSKNFFQSMRHSIRKIKSFDKKSNRNKSVEKVPSKSASAVIGSD
CDYFNFAKDTEGSELTKQSEEISIDLELEGGTPLKPRRQFTQEKYLTGSR
DVLKKAFNSLKRKSHMQDDKSLFDICSTVSYLFSNELEEMSFAKSEEKMF
QKFMTNCVNSFLDVHKIQIGTSLEMINRNIGEHVDLYSPNLDVINKSKKY
QITEIFGLKGTCIITNTGNKKKFIALKQGVKNWVLFTSFLQHIEQDTPLP
IHHQEWICFPAQSITNLYIEKNFFNPLHVECKLLLDSLIQLIVILYCQNI
HNIHIKLNPSNILIVSSNLSVIFVFIESDAGCASTDESQWLNLYDYSDKE
MRLAYQQSVMKLNKYVEKFLSLPENASHLITASYPLDYLFYLLLKLNKDI
NILLNSL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0003116parenchymal cell