SMED30017584

Overview
NameSMED30017584
Smed IDSMED30017584
Length (bp)1258
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30017584 (SMED30017584) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017584 (SMED30017584) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017584 (SMED30017584) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017584 (SMED30017584) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017584

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30017584SMESG000071184.1 dd_Smed_v4_13189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30017584SMESG000071184.1 dd_Smed_v4_13189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30017584SMESG000071184.1 dd_Smed_v4_13189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30017584SMESG000071184.1 dd_Smed_v4_13189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30017584SMESG000071184.1 dd_Smed_v4_13189_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30017584SMESG000071184.1 dd_Smed_v6_13189_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30017584 vs. TrEMBL
Match: A0A4S2MH70 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000928 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.468e-21
Identity = 92/252 (36.51%), Postives = 130/252 (51.59%), Query Frame = 1 
Query:  301 SLLPNNDIRRPSL-IQQ--------------SKEKLE-LHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAE--QKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICEC-GYYREITNHSISISYNKWNPLI-SYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQR 996
            +L PN   R P + I+Q              S+E    L  C+++VLLV+MQ+ LG S  ALG YLH   P L L++C +WAA PL  TG +G  FC     Q+  +   VSM  R  +   S++  +LC +AS FSG  GN +A     C +    T  K++          Q+  +P+   +C      +  H++    N  N +I SYH + C Y+ SS+KDYLILQ AL AVAAGV +W  V++  QR
Sbjct:  430 TLAPNTSFRFPGIDIEQGSPPSVGSPVCPPDSQETARGLCFCQLHVLLVIMQIVLGVSTTALGFYLHWKIPLLPLEECAFWAAIPLSFTGMIGSLFCFENRCQRFNRSYSVSM--RRATGILSLISCVLCLVASIFSGQKGNVIATSGHPCQSIRLTTQTKNAT---------QSSGLPEYEHQCCSDTSGLDGHNVCQCLNMRNQVIWSYHKITCRYLISSVKDYLILQSALMAVAAGVCLWFWVVLTEQR 670
[Close]
BLAST of SMED30017584 vs. TrEMBL
Match: A0A419Q0G8 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_3134s PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 2.950e-21
Identity = 79/212 (37.26%), Postives = 114/212 (53.77%), Query Frame = 1 
Query:  367 LHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICE-CGYYREITNHSISISYNKWNPLI-SYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQR 996
            L  C+++VLLV+MQ+ LG S  ALG YLH   P L L++C +WAA PL  TG +G  FC   +        S+ +R  +   +++  +LC +AS FSG  GN +A     C      T  K++          Q+  +P+   + C     +  H++    N  N +I SYH + C Y+ SS+KDYLILQ AL AVAAGV +W  V++  QR
Sbjct:  468 LCFCQLHVLLVIMQIVLGMSTTALGFYLHWKIPLLPLEECAFWAAIPLSFTGIIGSLFCFENRCQLFNRSYSVSMRRATGILTLISCVLCLVASIFSGQKGNVIASSGHPCQIVRPTTQTKNAT---------QSSGLPEYEHQCCSDTSSLDGHNVCQCLNMRNQVIWSYHKITCRYLISSVKDYLILQSALMAVAAGVCLWFWVVLTEQR 670
[Close]
BLAST of SMED30017584 vs. TrEMBL
Match: A0A4E0RIR9 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_000341 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.549e-20
Identity = 81/232 (34.91%), Postives = 121/232 (52.16%), Query Frame = 1 
Query:  367 LHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVC-PANLHETSEKHSIH--NHPTTSCCQTLQIPKLICECGYYREI-------TNHSISISYNKWNPLI-SYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQ-----RVHDQNK 1014
            L  C++ VLLV MQ+ LG +   LGLYL    P L L++C +WAA P+ +TG  G YFC   +   K   +++ +R  + A S++  +LC +AS FSG  G+ +A Y   C P+N       +SIH  +          Q  K   + G+ ++         +  +   +N  N L+ SY  + C Y+FSS+KDYLILQ AL AV  GV +W  V++  Q     R  ++NK
Sbjct:  535 LCFCQLIVLLVSMQISLGLAATGLGLYLRWRVPLLPLEECSFWAALPITITGLFGTYFCFQRRISRKNPQITLAIRRIAGALSLISCILCLVASIFSGQKGSLIATYGTDCIPSN-----PLYSIHNSSIKNNDNSNNNQEKKTDYDLGFEQQCCIDGVDRMSEPLCQCFNVRNQLMWSYRGIACRYLFSSVKDYLILQSALMAVGTGVCLWFWVVLTEQFQPVKRTDNENK 761
[Close]
BLAST of SMED30017584 vs. TrEMBL
Match: A0A1S3J0X6 (uncharacterized protein LOC106169229 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106169229 PE=4 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.638e-20
Identity = 99/334 (29.64%), Postives = 148/334 (44.31%), Query Frame = 1 
Query:   49 HQAMLEMNDNRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPD------KGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQ--------SKEKLELHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLG-LYFCSAEQKVFKKDLVSM-IVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICECGYYREITNHSISISYNKWNPLISY-HNVNCY-YVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQR 996
            H+ ML+ N  RPRA SAP+V        +I   ++ KK  + QP          N  TV    + +    +          + +          N+  RR S+ QQ        ++E  E+H CRI++LL  MQL LGS++  LG ++    P+L  ++ PYWA  PL L G  G ++FC AE + +      + +++      S +   +C +AS F G HG  +A Y                     T  C +T +    IC C    +          N    + +Y H ++C    F+S+KD LILQCAL A+  GVSIWL VL+W  R
Sbjct:   20 HKTMLD-NAGRPRAHSAPAV-----ATLSIVQPKKKKKCGQAQPSYLMELKPMLNGHTVHADTENNISTHDPTCRHHPRNQRSHHHHGNHHHHSNHHGRRMSMGQQVQRHFQHVTRESCEMHCCRIHILLTTMQLCLGSTIIGLGAFMGVAIPSLHTRETPYWAGIPLFLAGVFGAIFFCVAENRYYYSGSCKLFVIKAVVFTLSAISVFVCIVASIFPGIHGRRIAQY---------------------TGDCVRTNE----ICYCHLGDD----------NGMVRVYAYPHLLDCRPETFTSIKDNLILQCALNAIGCGVSIWLVVLLWQSR 312
[Close]
BLAST of SMED30017584 vs. TrEMBL
Match: A0A504YEC9 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_08303 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.953e-20
Identity = 82/241 (34.02%), Postives = 124/241 (51.45%), Query Frame = 1 
Query:  367 LHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVC-PAN----LH------ETSEKHSIHNHP------TTSCC--QTLQIPKLICECGYYREITNHSISISYNKWNPLI-SYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQ-----RVHDQNK 1014
            L  C++ VLLV MQ+ LG +   LGLYL    P L L++C +WAA P+ +TGF+G YFC   +   K   +++ +R  +   S++  +LC +AS FSG  G+ +A Y   C P+N    +H        +  +S+   P         CC     ++ + +C+C              +N  N L+ SY  + C Y+FSS+KDYLILQ AL AV  GV +W  V++  Q     R  ++NK
Sbjct:  536 LCFCQLIVLLVSMQISLGLAATGLGLYLRWRVPLLPLEECSFWAALPISITGFIGTYFCFQRRISRKNPQITLAIRRIAGVLSLISCILCLVASIFSGQKGSLIATYGTDCIPSNPLYSVHNSSIKNNNNSNNSLERKPDYDLGFEQQCCIDGVDRMSEPLCQC--------------FNVRNQLMWSYRGIACRYLFSSVKDYLILQSALMAVGTGVCLWFWVVLTEQFQPVKRTDNENK 762
[Close]
BLAST of SMED30017584 vs. Planmine SMEST
Match: SMESG000071184.1 (SMESG000071184.1)

HSP 1 Score: 740.725 bits (1911), Expect = 0.000e+0
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 1 
Query:   58 MLEMNDNRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRNSYARRRIKAMQDFINEDTDEISDHLNNNSVPPFANDCQTKITIGD 1170
            MLEMNDNRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQLGLGSSMAALGLYLHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRNSYARRRIKAMQDFINEDTDEISDHLNNNSVPPFANDCQTKITIGD
Sbjct:    1 MLEMNDNRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQLGLGSSMAALGLYLHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRNSYARRRIKAMQDFINEDTDEISDHLNNNSVPPFANDCQTKITIGD 371
[Close]
BLAST of SMED30017584 vs. Planmine SMEST
Match: SMESG000071184.1 (SMESG000071184.1)

HSP 1 Score: 733.406 bits (1892), Expect = 0.000e+0
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 1 
Query:   67 MNDNRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRNSYARRRIKAMQDFINEDTDEISDHLNNNSVPPFANDCQTKITIGD 1170
            MNDNRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQLGLGSSMAALGLYLHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRNSYARRRIKAMQDFINEDTDEISDHLNNNSVPPFANDCQTKITIGD
Sbjct:    1 MNDNRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQLGLGSSMAALGLYLHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRNSYARRRIKAMQDFINEDTDEISDHLNNNSVPPFANDCQTKITIGD 368
[Close]
BLAST of SMED30017584 vs. Planmine SMEST
Match: SMESG000063964.1 (SMESG000063964.1)

HSP 1 Score: 154.451 bits (389), Expect = 1.026e-42
Identity = 115/330 (34.85%), Postives = 178/330 (53.94%), Query Frame = 1 
Query:   79 RPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITVTVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKEKLELHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQ---TLQIPK--------LICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHDQNKNIIITRN 1035
            +PR +SAP V+  + K+    S+   +K+ K +     ++I++        D+  +  +   +   Y S  R  S++     RR S ++Q+KEK E+  CRINVLL+ MQLGLG SM+ LG+YL ++SP+LT KDC YWA  PLL +G  GLYFC ++ +++K+  +S +V+   A  S+V + L   AS  SG HG W++ +   C  +LH   E     ++P   CC+   T  +P+         +C C       N S+ + + +      Y  V C YVFS+LKDY ILQ +L  VA GV IW+ +L    ++ D+  N+I   N
Sbjct:   16 KPRVNSAPEVLAVINKKHK--SRGPVEKHLKHKRKSSQSSISI--------DVFRSPNNNFKNTESYLSISRNFSIVSG---RRLSSMEQNKEKFEIQFCRINVLLISMQLGLGFSMSGLGVYLRSVSPSLTWKDCTYWAGLPLLCSGAFGLYFCLSDHEIYKRSHLSRLVKIVCACMSVVTAALTLSASIMSGTHGKWISDFSSTC--DLHFIREHSETDSYP-DKCCEDKHTHPMPRHKLLPMNDTMCVCK-TAPFGNFSL-LKFER-----EYAAVTCRYVFSALKDYFILQSSLMGVATGVCIWIVIL----QIKDEQPNMIDQEN 318
[Close]
BLAST of SMED30017584 vs. Planmine SMEST
Match: SMESG000033408.1 (SMESG000033408.1)

HSP 1 Score: 119.783 bits (299), Expect = 1.616e-29
Identity = 78/225 (34.67%), Postives = 122/225 (54.22%), Query Frame = 1 
Query:  349 SKEKLELHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKL------ICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHD 1005
            S++ L+  +C+I VLLV M++ LGS+M  LG++L   S ++ +KDC +WA  PLL +G   ++  +   K+ K+  +  ++       S +CS+LC L+S FSG HGNW++ +   C       S+  +  N    +CC  +    +       C C   RE         Y+K    ++Y N+NC YVFS+LKDYLILQCAL  V +GV +WL  +   + + D
Sbjct:  169 SRDSLQHRLCKIRVLLVCMKILLGSTMTGLGIFLLMASKSIKVKDCAFWAGGPLLFSGLFEVFIYNWRNKLRKRMKICYLMTIACGVISFICSILCLLSSIFSGVHGNWISGHSDNC------LSKDDNNQNDMLLACCYDIHTKGINSSINSTCFCREARENI-------YSKPIITMTYENLNCRYVFSALKDYLILQCALMGVGSGVCLWLLQMTIQRLIQD 380
[Close]
BLAST of SMED30017584 vs. Planmine SMEST
Match: SMESG000033408.1 (SMESG000033408.1)

HSP 1 Score: 119.783 bits (299), Expect = 1.695e-29
Identity = 78/225 (34.67%), Postives = 122/225 (54.22%), Query Frame = 1 
Query:  349 SKEKLELHICRINVLLVMMQXXXXXXXXXXXXXXHNLSPALTLKDCPYWAAFPLLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFSGHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKL------ICECGYYREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAGVSIWLSVLIWSQRVHD 1005
            S++ L+  +C+I VLLV M++ LGS+M  LG++L   S ++ +KDC +WA  PLL +G   ++  +   K+ K+  +  ++       S +CS+LC L+S FSG HGNW++ +   C       S+  +  N    +CC  +    +       C C   RE         Y+K    ++Y N+NC YVFS+LKDYLILQCAL  V +GV +WL  +   + + D
Sbjct:  168 SRDSLQHRLCKIRVLLVCMKILLGSTMTGLGIFLLMASKSIKVKDCAFWAGGPLLFSGLFEVFIYNWRNKLRKRMKICYLMTIACGVISFICSILCLLSSIFSGVHGNWISGHSDNC------LSKDDNNQNDMLLACCYDIHTKGINSSINSTCFCREARENI-------YSKPIITMTYENLNCRYVFSALKDYLILQCALMGVGSGVCLWLLQMTIQRLIQD 379
The following BLAST results are available for this feature:
BLAST of SMED30017584 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2MH701.468e-2136.51Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A419Q0G82.950e-2137.26Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A4E0RIR92.549e-2034.91Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A1S3J0X62.638e-2029.64uncharacterized protein LOC106169229 isoform X1 OS... [more]
A0A504YEC92.953e-2034.02Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
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BLAST of SMED30017584 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017584 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000071184.10.000e+0100.00SMESG000071184.1[more]
SMESG000071184.10.000e+0100.00SMESG000071184.1[more]
SMESG000063964.11.026e-4234.85SMESG000063964.1[more]
SMESG000033408.11.616e-2934.67SMESG000033408.1[more]
SMESG000033408.11.695e-2934.67SMESG000033408.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017584 ID=SMED30017584|Name=SMED30017584|organism=Schmidtea mediterranea sexual|type=transcript|length=1258bp
GTCCAATTGAAAACAAGCAGGAAACCTTGTGAAATATAATTTTGATGACA
CCAGGCTATGTTAGAAATGAATGATAACCGACCAAGGGCTTCGTCTGCAC
CGTCAGTAATATTGCAAGTACAGAAACGGCAAAATATTTTCTCAAAACAA
CAAGATAAGAAAAATAAGAAAACTCAGCCTGATAAAGGAGCTAACAACAT
TACTGTGACAGTGAAATTTAAAGGATCAGATGATTTAATGGAAGCCGATA
TATCTGGTGACAATTCAGCGGGAAAATATTATTCAAAACTCCGTACGCTT
TCTCTTTTGCCAAATAATGACATCAGACGCCCTTCGTTGATTCAACAAAG
TAAAGAAAAGTTGGAATTACACATTTGCAGAATTAATGTCTTACTTGTAA
TGATGCAATTAGGACTTGGCAGTTCGATGGCAGCGTTGGGTCTTTACCTT
CATAACCTATCTCCAGCTCTTACTTTAAAAGACTGTCCGTATTGGGCTGC
ATTTCCTCTATTATTAACGGGATTTCTGGGTTTGTACTTTTGCTCTGCTG
AACAGAAAGTTTTCAAGAAAGATCTTGTGTCAATGATAGTTAGAAATACA
AGTGCTGCATTTTCTATTGTATGCTCTCTTCTATGTTCATTAGCAAGTGG
TTTTTCAGGACATCATGGTAACTGGCTAGCGTATTATTGTAAGGTTTGTC
CAGCAAATCTTCACGAAACCTCAGAAAAGCATTCGATTCACAACCATCCA
ACAACATCCTGTTGTCAAACATTGCAAATACCAAAACTCATTTGCGAATG
TGGATATTATCGTGAAATAACCAACCATTCTATTTCCATTTCATACAACA
AATGGAATCCGTTGATATCATATCACAATGTGAATTGTTATTACGTCTTC
TCGTCTCTCAAAGATTATCTCATTCTTCAATGTGCTCTTTATGCTGTAGC
GGCTGGGGTTTCAATTTGGTTGTCAGTTTTGATTTGGTCTCAAAGGGTTC
ATGATCAAAATAAAAACATCATAATAACAAGAAACAGTTACGCAAGACGA
CGCATTAAGGCGATGCAGGACTTTATTAATGAGGACACCGATGAAATTTC
CGATCATTTAAACAATAATTCTGTACCACCGTTCGCTAATGACTGTCAAA
CGAAAATCACAATTGGTGACTAAGGTTACAATTGAAAACAGACTTCAACC
GGGTTTTTAATCTTTCATATAATTTAGATTTGAAACAATGTCAAGATATA
GAAAAGAG
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protein sequence of SMED30017584-orf-1

>SMED30017584-orf-1 ID=SMED30017584-orf-1|Name=SMED30017584-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=372bp
MLEMNDNRPRASSAPSVILQVQKRQNIFSKQQDKKNKKTQPDKGANNITV
TVKFKGSDDLMEADISGDNSAGKYYSKLRTLSLLPNNDIRRPSLIQQSKE
KLELHICRINVLLVMMQLGLGSSMAALGLYLHNLSPALTLKDCPYWAAFP
LLLTGFLGLYFCSAEQKVFKKDLVSMIVRNTSAAFSIVCSLLCSLASGFS
GHHGNWLAYYCKVCPANLHETSEKHSIHNHPTTSCCQTLQIPKLICECGY
YREITNHSISISYNKWNPLISYHNVNCYYVFSSLKDYLILQCALYAVAAG
VSIWLSVLIWSQRVHDQNKNIIITRNSYARRRIKAMQDFINEDTDEISDH
LNNNSVPPFANDCQTKITIGD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR030429Sarcospan
Vocabulary: cellular component
TermDefinition
GO:0016010dystrophin-associated glycoprotein complex
GO:0005887integral component of plasma membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR030429SarcospanPANTHERPTHR15260FAMILY NOT NAMEDcoord: 97..319
NoneNo IPR availableTMHMMTMhelixcoord: 146..164
NoneNo IPR availableTMHMMTMhelixcoord: 177..199
NoneNo IPR availableTMHMMTMhelixcoord: 287..309
NoneNo IPR availableTMHMMTMhelixcoord: 109..131