SMED30017542

Overview
NameSMED30017542
Smed IDSMED30017542
Length (bp)1295
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30017542

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30017542SMESG000033620.1 dd_Smed_v4_22810_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30017542 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.017862:2163..3515 -6408
v31.017862:13242..14530 +5461
Homology
[Close]
BLAST of SMED30017542 vs. Ensembl Human
Match: POTEE (POTE ankyrin domain family member E [Source:HGNC Symbol;Acc:HGNC:33895])

HSP 1 Score: 70.4774 bits (171), Expect = 5.527e-18
Identity = 38/80 (47.50%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            ++  I T  DD+  IWH +FYN+LR  P EH +LLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:  771 MEHGIITNWDDMEKIWHHTFYNELRVAPEEHPILLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVPSLYTSGRTTG 850

HSP 2 Score: 40.4318 bits (93), Expect = 5.527e-18
Identity = 23/51 (45.10%), Postives = 31/51 (60.78%), Query Frame = -1 
Query:  282 LYISQKCTVGIVLDSGNGY----------ALSNTICRMDLSGRDLSEYLMK 404
            LY S + T GIV+DSG+G           AL +   R+DL+GR+L +YLMK
Sbjct:  842 LYTSGR-TTGIVMDSGDGVTHTVPIYEGNALPHATLRLDLAGRELPDYLMK 891
[Close]
BLAST of SMED30017542 vs. Ensembl Human
Match: ACTG1 (actin gamma 1 [Source:HGNC Symbol;Acc:HGNC:144])

HSP 1 Score: 75.0998 bits (183), Expect = 2.371e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Human
Match: ACTB (actin beta [Source:HGNC Symbol;Acc:HGNC:132])

HSP 1 Score: 74.7146 bits (182), Expect = 2.917e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Human
Match: ACTB (actin beta [Source:HGNC Symbol;Acc:HGNC:132])

HSP 1 Score: 74.7146 bits (182), Expect = 3.073e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Human
Match: ACTG1 (actin gamma 1 [Source:HGNC Symbol;Acc:HGNC:144])

HSP 1 Score: 75.0998 bits (183), Expect = 6.061e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150

HSP 2 Score: 48.521 bits (114), Expect = 6.438e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  140 LSLYASGRTT-GIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 191
[Close]
BLAST of SMED30017542 vs. Ensembl Celegans
Match: act-4 (Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986])

HSP 1 Score: 74.7146 bits (182), Expect = 1.378e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   28 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 107

HSP 2 Score: 48.1358 bits (113), Expect = 7.140e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T G+VLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:   97 LSLYASGRTT-GVVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 148
[Close]
BLAST of SMED30017542 vs. Ensembl Celegans
Match: act-4 (Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986])

HSP 1 Score: 74.7146 bits (182), Expect = 1.378e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   28 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 107

HSP 2 Score: 48.1358 bits (113), Expect = 7.140e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T G+VLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:   97 LSLYASGRTT-GVVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 148
[Close]
BLAST of SMED30017542 vs. Ensembl Celegans
Match: act-4 (Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986])

HSP 1 Score: 74.7146 bits (182), Expect = 1.378e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   28 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 107

HSP 2 Score: 48.1358 bits (113), Expect = 7.140e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T G+VLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:   97 LSLYASGRTT-GVVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 148
[Close]
BLAST of SMED30017542 vs. Ensembl Celegans
Match: act-4 (Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986])

HSP 1 Score: 74.7146 bits (182), Expect = 1.378e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   28 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 107

HSP 2 Score: 48.1358 bits (113), Expect = 7.140e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T G+VLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:   97 LSLYASGRTT-GVVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 148
[Close]
BLAST of SMED30017542 vs. Ensembl Celegans
Match: act-4 (Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986])

HSP 1 Score: 74.7146 bits (182), Expect = 1.378e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   28 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 107

HSP 2 Score: 48.1358 bits (113), Expect = 7.140e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T G+VLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:   97 LSLYASGRTT-GVVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 148
[Close]
BLAST of SMED30017542 vs. Ensembl Fly
Match: Act42A (gene:FBgn0000043 transcript:FBtr0086029)

HSP 1 Score: 74.7146 bits (182), Expect = 2.840e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 48.521 bits (114), Expect = 7.611e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGR-TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Fly
Match: Act5C (gene:FBgn0000042 transcript:FBtr0070822)

HSP 1 Score: 74.3294 bits (181), Expect = 2.892e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 48.521 bits (114), Expect = 7.885e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGR-TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Fly
Match: Act5C (gene:FBgn0000042 transcript:FBtr0100662)

HSP 1 Score: 74.3294 bits (181), Expect = 2.892e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 48.521 bits (114), Expect = 7.885e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGR-TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Fly
Match: Act5C (gene:FBgn0000042 transcript:FBtr0345894)

HSP 1 Score: 74.3294 bits (181), Expect = 2.892e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 48.521 bits (114), Expect = 7.885e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGR-TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Fly
Match: Act5C (gene:FBgn0000042 transcript:FBtr0070823)

HSP 1 Score: 74.3294 bits (181), Expect = 2.892e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 48.521 bits (114), Expect = 7.885e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGR-TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Zebrafish
Match: actb1 (actin, beta 1 [Source:NCBI gene;Acc:57934])

HSP 1 Score: 74.7146 bits (182), Expect = 6.301e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150

HSP 2 Score: 48.1358 bits (113), Expect = 5.998e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  140 LSLYASGRTT-GIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 191
[Close]
BLAST of SMED30017542 vs. Ensembl Zebrafish
Match: actb1 (actin, beta 1 [Source:NCBI gene;Acc:57934])

HSP 1 Score: 74.3294 bits (181), Expect = 2.280e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Zebrafish
Match: actc1a (actin alpha cardiac muscle 1a [Source:ZFIN;Acc:ZDB-GENE-040520-4])

HSP 1 Score: 74.7146 bits (182), Expect = 3.485e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Zebrafish
Match: acta2 (actin alpha 2, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-030131-1229])

HSP 1 Score: 74.3294 bits (181), Expect = 3.713e-14
Identity = 40/80 (50.00%), Postives = 47/80 (58.75%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH SFYN+LR  P EH  LLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   30 IEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 109

HSP 2 Score: 48.521 bits (114), Expect = 8.482e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:   99 LSLYASGRTT-GIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMK 150
[Close]
BLAST of SMED30017542 vs. Ensembl Zebrafish
Match: actb1 (actin, beta 1 [Source:NCBI gene;Acc:57934])

HSP 1 Score: 74.7146 bits (182), Expect = 3.749e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Xenopus
Match: actg1 (actin gamma 1 [Source:NCBI gene;Acc:394957])

HSP 1 Score: 74.7146 bits (182), Expect = 5.012e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Xenopus
Match: actb (actin, beta [Source:NCBI gene;Acc:407952])

HSP 1 Score: 74.3294 bits (181), Expect = 5.440e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Xenopus
Match: acta2 (actin, alpha 2, smooth muscle, aorta [Source:NCBI gene;Acc:496696])

HSP 1 Score: 73.9442 bits (180), Expect = 7.712e-14
Identity = 40/80 (50.00%), Postives = 47/80 (58.75%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH SFYN+LR  P EH  LLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   73 IEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 152
[Close]
BLAST of SMED30017542 vs. Ensembl Xenopus
Match: acta2 (actin, alpha 2, smooth muscle, aorta [Source:NCBI gene;Acc:496696])

HSP 1 Score: 73.9442 bits (180), Expect = 8.075e-14
Identity = 40/80 (50.00%), Postives = 47/80 (58.75%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH SFYN+LR  P EH  LLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   70 IEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 149
[Close]
BLAST of SMED30017542 vs. Ensembl Xenopus
Match: acta2 (actin, alpha 2, smooth muscle, aorta [Source:NCBI gene;Acc:496696])

HSP 1 Score: 73.9442 bits (180), Expect = 8.373e-14
Identity = 40/80 (50.00%), Postives = 47/80 (58.75%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH SFYN+LR  P EH  LLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   73 IEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 152
[Close]
BLAST of SMED30017542 vs. Ensembl Mouse
Match: Actb (actin, beta [Source:MGI Symbol;Acc:MGI:87904])

HSP 1 Score: 73.9442 bits (180), Expect = 2.418e-15
Identity = 39/75 (52.00%), Postives = 46/75 (61.33%), Query Frame = -3 
Query:  391 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKS 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S
Sbjct:   74 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAS 148
[Close]
BLAST of SMED30017542 vs. Ensembl Mouse
Match: Actg2 (actin, gamma 2, smooth muscle, enteric [Source:MGI Symbol;Acc:MGI:104589])

HSP 1 Score: 73.9442 bits (180), Expect = 7.732e-15
Identity = 40/80 (50.00%), Postives = 47/80 (58.75%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH SFYN+LR  P EH  LLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 48.521 bits (114), Expect = 3.807e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGR-TTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Mouse
Match: Actg1 (actin, gamma, cytoplasmic 1 [Source:MGI Symbol;Acc:MGI:87906])

HSP 1 Score: 75.0998 bits (183), Expect = 9.457e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:  128 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 207

HSP 2 Score: 48.521 bits (114), Expect = 6.290e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  197 LSLYASGRTT-GIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 248
[Close]
BLAST of SMED30017542 vs. Ensembl Mouse
Match: Acta2 (actin, alpha 2, smooth muscle, aorta [Source:MGI Symbol;Acc:MGI:87909])

HSP 1 Score: 73.9442 bits (180), Expect = 1.117e-14
Identity = 40/80 (50.00%), Postives = 47/80 (58.75%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH SFYN+LR  P EH  LLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   73 IEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 152

HSP 2 Score: 48.521 bits (114), Expect = 4.461e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  142 LSLYASGRTT-GIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMK 193
[Close]
BLAST of SMED30017542 vs. Ensembl Mouse
Match: Acta1 (actin, alpha 1, skeletal muscle [Source:MGI Symbol;Acc:MGI:87902])

HSP 1 Score: 72.0182 bits (175), Expect = 1.174e-14
Identity = 38/75 (50.67%), Postives = 45/75 (60.00%), Query Frame = -3 
Query:  391 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKS 588
            I+  I T  DD+  IWH +FYN+LR  P EH  LLT+A LNPK +RE  TQIM +         AIQA  SLY S
Sbjct:   73 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYAS 147
[Close]
BLAST of SMED30017542 vs. UniProt/SwissProt
Match: sp|Q6S8J3|POTEE_HUMAN (POTE ankyrin domain family member E OS=Homo sapiens OX=9606 GN=POTEE PE=2 SV=3)

HSP 1 Score: 70.4774 bits (171), Expect = 2.398e-17
Identity = 38/80 (47.50%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            ++  I T  DD+  IWH +FYN+LR  P EH +LLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:  771 MEHGIITNWDDMEKIWHHTFYNELRVAPEEHPILLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVPSLYTSGRTTG 850

HSP 2 Score: 40.4318 bits (93), Expect = 2.398e-17
Identity = 23/51 (45.10%), Postives = 31/51 (60.78%), Query Frame = -1 
Query:  282 LYISQKCTVGIVLDSGNGY----------ALSNTICRMDLSGRDLSEYLMK 404
            LY S + T GIV+DSG+G           AL +   R+DL+GR+L +YLMK
Sbjct:  842 LYTSGR-TTGIVMDSGDGVTHTVPIYEGNALPHATLRLDLAGRELPDYLMK 891
[Close]
BLAST of SMED30017542 vs. UniProt/SwissProt
Match: sp|P24263|ACTD_PHYPO (Actin, spherule isoform OS=Physarum polycephalum OX=5791 GN=ARDD PE=2 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 6.198e-16
Identity = 37/80 (46.25%), Postives = 47/80 (58.75%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I++ I    DD+  IWH +FYN++R  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SL  S   +G
Sbjct:   69 IEQGIVNNWDDMEQIWHHTFYNEMRVAPEEHPVLLTEAPLNPKANREKMTQIMFESFSAPAMYVAIQAVLSLRASGRTTG 148

HSP 2 Score: 37.3502 bits (85), Expect = 6.198e-16
Identity = 20/42 (47.62%), Postives = 25/42 (59.52%), Query Frame = -1 
Query:  288 TVGIVLDSGNGY----------ALSNTICRMDLSGRDLSEYL 383
            T GIVLDSG+G           AL + I R+D +GRDL+ YL
Sbjct:  146 TTGIVLDSGDGASHAVPIYEGCALPHAILRIDAAGRDLTHYL 187
[Close]
BLAST of SMED30017542 vs. UniProt/SwissProt
Match: sp|P30169|ACT7_SOLTU (Actin-75 OS=Solanum tuberosum OX=4113 GN=AC75 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.333e-15
Identity = 28/44 (63.64%), Postives = 33/44 (75.00%), Query Frame = -3 
Query:  430 DDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIM 561
            DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM
Sbjct:   82 DDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 125

HSP 2 Score: 39.6614 bits (91), Expect = 2.333e-15
Identity = 20/40 (50.00%), Postives = 26/40 (65.00%), Query Frame = -1 
Query:  282 VLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 371
            VLDSG+G          YAL + I R+DL+GRDL+E+L K
Sbjct:  154 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTEHLAK 193
[Close]
BLAST of SMED30017542 vs. UniProt/SwissProt
Match: sp|Q11212|ACT_SPOLI (Actin (Fragment) OS=Spodoptera littoralis OX=7109 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.317e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   62 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG 141
[Close]
BLAST of SMED30017542 vs. UniProt/SwissProt
Match: sp|P84336|ACTB_CAMDR (Actin, cytoplasmic 1 OS=Camelus dromedarius OX=9838 GN=ACTB PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.558e-14
Identity = 44/96 (45.83%), Postives = 56/96 (58.33%), Query Frame = -3 
Query:  376 GEIIKDEKCIY-----IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 621
            G+  + ++CI      I+R I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   55 GDEAQSKRCILTLKYPIERGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. TrEMBL
Match: F7E7I5 (Uncharacterized protein OS=Ornithorhynchus anatinus OX=9258 PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.191e-19
Identity = 39/75 (52.00%), Postives = 45/75 (60.00%), Query Frame = -3 
Query:  391 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKS 588
            I+  I T  DD+  IWH SFYN+LR  P EH  LLT+A LNPK +RE  TQIM +         AIQA  SLY S
Sbjct:   31 IEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYAS 105

HSP 2 Score: 52.7582 bits (125), Expect = 1.191e-19
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  100 LSLYASGRTTXGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMK 152
[Close]
BLAST of SMED30017542 vs. TrEMBL
Match: A0A0G4AMA0 (Actin OS=Lucilia sericata OX=13632 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 5.323e-19
Identity = 41/80 (51.25%), Postives = 49/80 (61.25%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA FSLY S   +G
Sbjct:   72 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVFSLYASGRTTG 151

HSP 2 Score: 47.7506 bits (112), Expect = 5.323e-19
Identity = 27/51 (52.94%), Postives = 34/51 (66.67%), Query Frame = -1 
Query:  282 LYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 404
            LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  143 LYASGR-TTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. TrEMBL
Match: D9YST6 (Actin (Fragment) OS=Anopheles quadrimaculatus OX=7166 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.470e-13
Identity = 41/80 (51.25%), Postives = 49/80 (61.25%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIMC+         AIQA  SLY S   +G
Sbjct:    9 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMCETFNSPAMYVAIQAGLSLYASGRTTG 88
[Close]
BLAST of SMED30017542 vs. TrEMBL
Match: A0A076FQY7 (Beta-actin (Fragment) OS=Thunnus alalunga OX=8235 GN=Actb PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 4.908e-13
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   11 IEHGIITNRDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 90
[Close]
BLAST of SMED30017542 vs. TrEMBL
Match: A0A443QLF2 (Putative beta-actin-like protein (Fragment) OS=Leptotrombidium deliense OX=299467 GN=B4U80_11349 PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 8.862e-13
Identity = 41/80 (51.25%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH SFYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   28 IEHGIITNWDDMEKIWHHSFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 107
[Close]
BLAST of SMED30017542 vs. Ensembl Cavefish
Match: ENSAMXT00000008835.2 (pep primary_assembly:Astyanax_mexicanus-2.0:18:331874:339765:-1 gene:ENSAMXG00000008598.2 transcript:ENSAMXT00000008835.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.7146 bits (182), Expect = 2.564e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   73 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 152
[Close]
BLAST of SMED30017542 vs. Ensembl Cavefish
Match: actb1 (actin, cytoplasmic 1 [Source:NCBI gene;Acc:103039558])

HSP 1 Score: 74.7146 bits (182), Expect = 3.177e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Cavefish
Match: actc1c (actin, alpha cardiac muscle 1 [Source:NCBI gene;Acc:103039206])

HSP 1 Score: 74.7146 bits (182), Expect = 3.186e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   73 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG 152
[Close]
BLAST of SMED30017542 vs. Ensembl Cavefish
Match: actb2 (actin beta [Source:NCBI gene;Acc:103030443])

HSP 1 Score: 74.3294 bits (181), Expect = 3.206e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Cavefish
Match: actb1 (actin, cytoplasmic 1 [Source:NCBI gene;Acc:103039558])

HSP 1 Score: 74.3294 bits (181), Expect = 3.245e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   73 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 152
[Close]
BLAST of SMED30017542 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011113.1 (pep scaffold:Pmarinus_7.0:GL498954:115:1245:1 gene:ENSPMAG00000010086.1 transcript:ENSPMAT00000011113.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.3294 bits (181), Expect = 6.543e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 47.7506 bits (112), Expect = 2.726e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGR-TTGIVMDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004586.1 (pep scaffold:Pmarinus_7.0:GL479516:23873:27955:-1 gene:ENSPMAG00000004161.1 transcript:ENSPMAT00000004586.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.3294 bits (181), Expect = 6.663e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 47.7506 bits (112), Expect = 2.849e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGR-TTGIVMDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011230.1 (pep scaffold:Pmarinus_7.0:GL476986:121012:122166:1 gene:ENSPMAG00000010203.1 transcript:ENSPMAT00000011230.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 9.330e-9
Identity = 28/80 (35.00%), Postives = 44/80 (55.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            ++R I T  DD+  +W  ++  +L A P +  VLLT+A LNP  +RE   Q++ +         A+QA  SLY S + +G
Sbjct:   82 VERGIVTRWDDMERVWAHAYEAELHARPQDQPVLLTEAPLNPLENREQAAQLLFERFGAPAVHIAVQAVLSLYASGMVTG 161

HSP 2 Score: 25.7942 bits (55), Expect = 9.330e-9
Identity = 9/23 (39.13%), Postives = 15/23 (65.22%), Query Frame = -1 
Query:  288 NGYALSNTICRMDLSGRDLSEYL 356
             GY L   + R D++GRD+++ L
Sbjct:  178 EGYGLPRAVLRSDVAGRDVTDNL 200
[Close]
BLAST of SMED30017542 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008200.1 (pep scaffold:Pmarinus_7.0:GL477804:3783:17322:1 gene:ENSPMAG00000007412.1 transcript:ENSPMAT00000008200.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 35.8094 bits (81), Expect = 3.923e-7
Identity = 18/53 (33.96%), Postives = 30/53 (56.60%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY       G+V+DSG+G          ++L +   R+D++GRD++ YL+K
Sbjct:  128 LTLYAQAGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIK 180

HSP 2 Score: 35.039 bits (79), Expect = 3.923e-7
Identity = 21/65 (32.31%), Postives = 34/65 (52.31%), Query Frame = -3 
Query:  397 DDLGNIWHCSFY-NKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLY 561
            DD+ ++W  +F  +KL     E  +LLT+  +NP  +RE   ++M +         AIQA  +LY
Sbjct:   67 DDMKHLWDYTFGPDKLNVNTRECKILLTEPPMNPMKNREKIIEVMFEQYGFSAVYIAIQAVLTLY 131
[Close]
BLAST of SMED30017542 vs. Ensembl Yeast
Match: ACT1 (Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions [Source:SGD;Acc:S000001855])

HSP 1 Score: 73.559 bits (179), Expect = 8.219e-15
Identity = 38/80 (47.50%), Postives = 49/80 (61.25%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A +NPK++RE  TQIM +         +IQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Nematostella
Match: EDO27265 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T9Q8])

HSP 1 Score: 73.559 bits (179), Expect = 4.013e-16
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   10 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG 89
[Close]
BLAST of SMED30017542 vs. Ensembl Nematostella
Match: EDO38006 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEB1])

HSP 1 Score: 74.7146 bits (182), Expect = 9.320e-15
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG 150

HSP 2 Score: 48.1358 bits (113), Expect = 3.756e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  140 LSLYASGRTT-GIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 191
[Close]
BLAST of SMED30017542 vs. Ensembl Nematostella
Match: EDO45013 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RU31])

HSP 1 Score: 74.7146 bits (182), Expect = 1.012e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG 150

HSP 2 Score: 47.7506 bits (112), Expect = 4.602e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  140 LSLYASGRTT-GIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 191
[Close]
BLAST of SMED30017542 vs. Ensembl Nematostella
Match: EDO42646 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0V7])

HSP 1 Score: 74.7146 bits (182), Expect = 1.069e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG 150

HSP 2 Score: 48.1358 bits (113), Expect = 4.139e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  140 LSLYASGRTT-GIVMDSGDGVTHTVPIYEGYALPHAIIRLDLAGRDLTDYLMK 191
[Close]
BLAST of SMED30017542 vs. Ensembl Nematostella
Match: EDO37976 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEA7])

HSP 1 Score: 74.7146 bits (182), Expect = 1.075e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 47.7506 bits (112), Expect = 4.778e-6
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV+DSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGRTT-GIVMDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Ensembl Medaka
Match: ENSORLT00000021723.2 (actin-15A [Source:NCBI gene;Acc:101155777])

HSP 1 Score: 72.4034 bits (176), Expect = 7.747e-20
Identity = 38/75 (50.67%), Postives = 45/75 (60.00%), Query Frame = -3 
Query:  391 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKS 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE   QIM +         AIQA  SLY S
Sbjct:   68 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMAQIMFETFNSPAMYVAIQAVLSLYAS 142

HSP 2 Score: 44.2838 bits (103), Expect = 7.747e-20
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV++SG+G          YAL++ I R++L+GRDL++YLMK
Sbjct:  137 LSLYASGRIT-GIVMESGDGVSQIVPIYEGYALTHAILRLNLAGRDLTDYLMK 188
[Close]
BLAST of SMED30017542 vs. Ensembl Medaka
Match: ENSORLT00000021734.2 (actin-15A [Source:NCBI gene;Acc:101155777])

HSP 1 Score: 72.4034 bits (176), Expect = 8.205e-20
Identity = 38/75 (50.67%), Postives = 45/75 (60.00%), Query Frame = -3 
Query:  391 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKS 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE   QIM +         AIQA  SLY S
Sbjct:   68 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMAQIMFETFNSPAMYVAIQAVLSLYAS 142

HSP 2 Score: 44.2838 bits (103), Expect = 8.205e-20
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIV++SG+G          YAL++ I R++L+GRDL++YLMK
Sbjct:  137 LSLYASGRIT-GIVMESGDGVSQIVPIYEGYALTHAILRLNLAGRDLTDYLMK 188
[Close]
BLAST of SMED30017542 vs. Ensembl Medaka
Match: ENSORLT00000021483.2 (actin, cytoplasmic-like [Source:NCBI gene;Acc:101169951])

HSP 1 Score: 70.0922 bits (170), Expect = 2.333e-19
Identity = 36/73 (49.32%), Postives = 45/73 (61.64%), Query Frame = -3 
Query:  397 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLY 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  T++M +         AIQA  SLY
Sbjct:   26 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTEVMFETFNSPAMYLAIQAVLSLY 98

HSP 2 Score: 45.0542 bits (105), Expect = 2.333e-19
Identity = 22/44 (50.00%), Postives = 29/44 (65.91%), Query Frame = -1 
Query:  282 TVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 383
            T GIV+DSG+G          YAL +   R+DL+GRDL++YLMK
Sbjct:  103 TTGIVMDSGDGVSQTVPIYEGYALPHATIRLDLAGRDLTDYLMK 146
[Close]
BLAST of SMED30017542 vs. Ensembl Medaka
Match: ENSORLT00000021726.2 (actin, cytoplasmic [Source:NCBI gene;Acc:101155523])

HSP 1 Score: 74.7146 bits (182), Expect = 2.804e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Ensembl Medaka
Match: ENSORLT00000029375.1 (actin, cytoplasmic 2 [Source:NCBI gene;Acc:101168921])

HSP 1 Score: 74.7146 bits (182), Expect = 2.962e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 150
[Close]
BLAST of SMED30017542 vs. Planmine SMEST
Match: SMESG000033620.1 (SMESG000033620.1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.210e-19
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = -3 
Query: 1177 RRRLHWHAVYDDKNSLAHKIENLYLTNLGWCFSYGLI 1287
            RRRLHWHAVYDDKNSLAHKIENLYLTNLGWCFSYGLI
Sbjct:   51 RRRLHWHAVYDDKNSLAHKIENLYLTNLGWCFSYGLI 87
[Close]
BLAST of SMED30017542 vs. Planmine SMEST
Match: SMESG000033620.1 (SMESG000033620.1)

HSP 1 Score: 81.6481 bits (200), Expect = 4.741e-19
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = -3 
Query: 1177 RRRLHWHAVYDDKNSLAHKIENLYLTNLGWCFSYGLI 1287
            RRRLHWHAVYDDKNSLAHKIENLYLTNLGWCFSYGLI
Sbjct:   43 RRRLHWHAVYDDKNSLAHKIENLYLTNLGWCFSYGLI 79
[Close]
BLAST of SMED30017542 vs. Planmine SMEST
Match: SMESG000035518.1 (SMESG000035518.1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.796e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   71 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNCPAMYVAIQAVLSLYASGRTTG 150

HSP 2 Score: 52.373 bits (124), Expect = 3.167e-7
Identity = 29/53 (54.72%), Postives = 36/53 (67.92%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + ICR+DL+GRDL++YLMK
Sbjct:  140 LSLYASGR-TTGIVLDSGDGVTHTVPIYEGYALPHAICRLDLAGRDLTDYLMK 191
[Close]
BLAST of SMED30017542 vs. Planmine SMEST
Match: SMESG000066020.1 (SMESG000066020.1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.084e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 48.521 bits (114), Expect = 5.606e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGRTT-GIVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
[Close]
BLAST of SMED30017542 vs. Planmine SMEST
Match: SMESG000066029.1 (SMESG000066029.1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.142e-14
Identity = 40/80 (50.00%), Postives = 48/80 (60.00%), Query Frame = -3 
Query:  376 IQRDIWTYNDDLGNIWHCSFYNKLRAVPVEHSVLLTKAQLNPKTDRETFTQIMCD---------AIQAAFSLYKSEVHSG 588
            I+  I T  DD+  IWH +FYN+LR  P EH VLLT+A LNPK +RE  TQIM +         AIQA  SLY S   +G
Sbjct:   72 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 151

HSP 2 Score: 48.521 bits (114), Expect = 5.460e-6
Identity = 28/53 (52.83%), Postives = 35/53 (66.04%), Query Frame = -1 
Query:  282 LFLYISQKCTVGIVLDSGNG----------YALSNTICRMDLSGRDLSEYLMK 410
            L LY S + T GIVLDSG+G          YAL + I R+DL+GRDL++YLMK
Sbjct:  141 LSLYASGRTT-GIVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 192
The following BLAST results are available for this feature:
BLAST of SMED30017542 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
POTEE5.527e-1847.50POTE ankyrin domain family member E [Source:HGNC S... [more]
ACTG12.371e-1550.00actin gamma 1 [Source:HGNC Symbol;Acc:HGNC:144][more]
ACTB2.917e-1550.00actin beta [Source:HGNC Symbol;Acc:HGNC:132][more]
ACTB3.073e-1550.00actin beta [Source:HGNC Symbol;Acc:HGNC:132][more]
ACTG16.061e-1550.00actin gamma 1 [Source:HGNC Symbol;Acc:HGNC:144][more]
back to top
BLAST of SMED30017542 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
act-41.378e-1450.00Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986][more]
act-41.378e-1450.00Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986][more]
act-41.378e-1450.00Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986][more]
act-41.378e-1450.00Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986][more]
act-41.378e-1450.00Actin-4 [Source:UniProtKB/Swiss-Prot;Acc:P10986][more]
back to top
BLAST of SMED30017542 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Act42A2.840e-1450.00gene:FBgn0000043 transcript:FBtr0086029[more]
Act5C2.892e-1450.00gene:FBgn0000042 transcript:FBtr0070822[more]
Act5C2.892e-1450.00gene:FBgn0000042 transcript:FBtr0100662[more]
Act5C2.892e-1450.00gene:FBgn0000042 transcript:FBtr0345894[more]
Act5C2.892e-1450.00gene:FBgn0000042 transcript:FBtr0070823[more]
back to top
BLAST of SMED30017542 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
actb16.301e-1550.00actin, beta 1 [Source:NCBI gene;Acc:57934][more]
actb12.280e-1450.00actin, beta 1 [Source:NCBI gene;Acc:57934][more]
actc1a3.485e-1450.00actin alpha cardiac muscle 1a [Source:ZFIN;Acc:ZDB... [more]
acta23.713e-1450.00actin alpha 2, smooth muscle [Source:ZFIN;Acc:ZDB-... [more]
actb13.749e-1450.00actin, beta 1 [Source:NCBI gene;Acc:57934][more]
back to top
BLAST of SMED30017542 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
actg15.012e-1450.00actin gamma 1 [Source:NCBI gene;Acc:394957][more]
actb5.440e-1450.00actin, beta [Source:NCBI gene;Acc:407952][more]
acta27.712e-1450.00actin, alpha 2, smooth muscle, aorta [Source:NCBI ... [more]
acta28.075e-1450.00actin, alpha 2, smooth muscle, aorta [Source:NCBI ... [more]
acta28.373e-1450.00actin, alpha 2, smooth muscle, aorta [Source:NCBI ... [more]
back to top
BLAST of SMED30017542 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Actb2.418e-1552.00actin, beta [Source:MGI Symbol;Acc:MGI:87904][more]
Actg27.732e-1550.00actin, gamma 2, smooth muscle, enteric [Source:MGI... [more]
Actg19.457e-1550.00actin, gamma, cytoplasmic 1 [Source:MGI Symbol;Acc... [more]
Acta21.117e-1450.00actin, alpha 2, smooth muscle, aorta [Source:MGI S... [more]
Acta11.174e-1450.67actin, alpha 1, skeletal muscle [Source:MGI Symbol... [more]
back to top
BLAST of SMED30017542 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6S8J3|POTEE_HUMAN2.398e-1747.50POTE ankyrin domain family member E OS=Homo sapien... [more]
sp|P24263|ACTD_PHYPO6.198e-1646.25Actin, spherule isoform OS=Physarum polycephalum O... [more]
sp|P30169|ACT7_SOLTU2.333e-1563.64Actin-75 OS=Solanum tuberosum OX=4113 GN=AC75 PE=3... [more]
sp|Q11212|ACT_SPOLI1.317e-1450.00Actin (Fragment) OS=Spodoptera littoralis OX=7109 ... [more]
sp|P84336|ACTB_CAMDR1.558e-1445.83Actin, cytoplasmic 1 OS=Camelus dromedarius OX=983... [more]
back to top
BLAST of SMED30017542 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
F7E7I51.191e-1952.00Uncharacterized protein OS=Ornithorhynchus anatinu... [more]
A0A0G4AMA05.323e-1951.25Actin OS=Lucilia sericata OX=13632 PE=2 SV=1[more]
D9YST62.470e-1351.25Actin (Fragment) OS=Anopheles quadrimaculatus OX=7... [more]
A0A076FQY74.908e-1350.00Beta-actin (Fragment) OS=Thunnus alalunga OX=8235 ... [more]
A0A443QLF28.862e-1351.25Putative beta-actin-like protein (Fragment) OS=Lep... [more]
back to top
BLAST of SMED30017542 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000008835.22.564e-1450.00pep primary_assembly:Astyanax_mexicanus-2.0:18:331... [more]
actb13.177e-1450.00actin, cytoplasmic 1 [Source:NCBI gene;Acc:1030395... [more]
actc1c3.186e-1450.00actin, alpha cardiac muscle 1 [Source:NCBI gene;Ac... [more]
actb23.206e-1450.00actin beta [Source:NCBI gene;Acc:103030443][more]
actb13.245e-1450.00actin, cytoplasmic 1 [Source:NCBI gene;Acc:1030395... [more]
back to top
BLAST of SMED30017542 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSPMAT00000011113.16.543e-1550.00pep scaffold:Pmarinus_7.0:GL498954:115:1245:1 gene... [more]
ENSPMAT00000004586.16.663e-1550.00pep scaffold:Pmarinus_7.0:GL479516:23873:27955:-1 ... [more]
ENSPMAT00000011230.19.330e-935.00pep scaffold:Pmarinus_7.0:GL476986:121012:122166:1... [more]
ENSPMAT00000008200.13.923e-733.96pep scaffold:Pmarinus_7.0:GL477804:3783:17322:1 ge... [more]
back to top
BLAST of SMED30017542 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
ACT18.219e-1547.50Actin; structural protein involved in cell polariz... [more]
back to top
BLAST of SMED30017542 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO272654.013e-1650.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO380069.320e-1550.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO450131.012e-1450.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO426461.069e-1450.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO379761.075e-1450.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMED30017542 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000021723.27.747e-2050.67actin-15A [Source:NCBI gene;Acc:101155777][more]
ENSORLT00000021734.28.205e-2050.67actin-15A [Source:NCBI gene;Acc:101155777][more]
ENSORLT00000021483.22.333e-1949.32actin, cytoplasmic-like [Source:NCBI gene;Acc:1011... [more]
ENSORLT00000021726.22.804e-1450.00actin, cytoplasmic [Source:NCBI gene;Acc:101155523... [more]
ENSORLT00000029375.12.962e-1450.00actin, cytoplasmic 2 [Source:NCBI gene;Acc:1011689... [more]
back to top
BLAST of SMED30017542 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000033620.14.210e-19100.00SMESG000033620.1[more]
SMESG000033620.14.741e-19100.00SMESG000033620.1[more]
SMESG000035518.11.796e-1450.00SMESG000035518.1[more]
SMESG000066020.12.084e-1450.00SMESG000066020.1[more]
SMESG000066029.12.142e-1450.00SMESG000066029.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017542 ID=SMED30017542|Name=SMED30017542|organism=Schmidtea mediterranea sexual|type=transcript|length=1295bp
CATTTCACACCCTTCTCCATTCAATCATTTTTACCAAAGCTCATGAACGA
TTTAATATTGTTCCTGGGTTCGCTTAATTTGAATATTTTACATGTTGCAT
TTGGTGAACGTAATTTATTTTTCTTGAATGGGTTTTGCATTTTATATTTT
ATGTAAAATTTGACTTTGTAACTAAAAAGGTATTAATTAAATTTGTAGTT
GATCTTTTGGATTTAATTTCACTGTTGATCTCTAGTTTGTTAGATATAGC
AGTGAAAATGAACCCTCGTCCAGTCAGTTTTTTTCATCAAATATTCTGAC
AAATCTCTACCAGATAGATCCATTCTACAAATTGTATTAGACAACGCATA
ACCATTTCCGGAATCAAGAACGATACCCACTGTGCACTTCTGACTTATAT
AAAGAAAAAGCTGCTTGAATTGCATCACACATAATTTGAGTAAAAGTTTC
TCGGTCTGTCTTTGGATTCAACTGAGCTTTAGTCAACAAAACTGAATGTT
CTACTGGTACTGCTCTCAGTTTATTATAAAATGAGCAATGCCAAATATTT
CCCAAATCATCATTATATGTCCAAATATCGCGTTGAATATAGATACATTT
TTCATCCTTTATTATTTCCCCATATGATTCAGTAAAATTCGACGATAGAA
AAATTCAAGTAGACATTTTCGTAACCAGAAGATTAAATAAATAACGTAAA
AAGTGTTTACTTCAAATGCAAATTAACCAAAAGAACCCATAAATTCAAAT
TCCGTCTAAAGTTCTTTAATTTCGAAAAACTAAATTAAATATAATAAACC
AATTGAATCTTGTCAAAATCAAAAGTATAAAAAAGCCACAAAATCGCATT
ACGCTTACAGGCGCTAAACATAATCACAAGAAAGCGCACACAGACACGAT
GAAAAATAAATATTTGAATGTCCTTTCAACTATCAAATCCTTTTCCATCT
TTATTTTCCTTCACAACAATTTCTTTATTTTCTTCGATTCTCACTGTTTA
GGTTTTGCTGGAATTTTCACTTTCTGGCATCTCAGAGTCATGTATTCTTC
GTTATATGTATATATTTAGGTTTTTTCCAGGACTAGACACCAACATAATG
AAGGTCTAGTGATGAAATTTGGGGGGAATGTCTTGGCTGTTTAAGTTACC
GATCTTGTAGCTAGTCCACCTGGCTAAATTAGTCCGTATGAAAAACACCA
TCCAAGATTGGTCAGATATAAATTTTCTATCTTGTGTGCAAGTGAATTTT
TATCATCATAAACTGCATGCCAGTGAAGGCGTCTTCTGTTTTGAA
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
Vocabulary: INTERPRO
TermDefinition
IPR004000Actin
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.420.40coord: 7..36
e-value: 2.2E-5
score: 25.9
NoneNo IPR availableGENE3DG3DSA:3.90.640.10coord: 37..48
e-value: 2.2E-5
score: 25.9