SMED30017519
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30017519 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30017519 aligns in the following genomic locations:
Homology
BLAST of SMED30017519 vs. Planmine SMEST
Match: SMESG000055195.1 (SMESG000055195.1) HSP 1 Score: 684.1 bits (1764), Expect = 0.000e+0 Identity = 352/496 (70.97%), Postives = 392/496 (79.03%), Query Frame = 3 Query: 6 FCSFLMDNFKCYFFILGFVNLISIFSSSYLYAEYNKTFRKMERIVFNISFRDFGVFLLYATDSDGFKLLIEAGNLYKTKEIYFNFNGHLFNKSITNFSNFINHFDVVLTFQSNSLKYDENGVISDIFYMKPFFPDAFRNYPVPKDFTNLKNKVYFWIGNIGRYVGFKGRIYEPFTTPTFGCLNSLYVDDYSLEFIKEAEYFSRTCLHLVDEHLCVKDNCSLIKTKVNFLDCSFPSVNTKRMCYKDLIRTDNQSVIYKKFPDSNESTSFKIIWKQERFEKFCLTEIEMXXXXXXXXXXXXXXXRDGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFKLICPSKIPSTPHKIYFFLSLIYDAMMTFLFAVLCTLRFCSNSKQKVVKITSPIEINNNETTYVEETGDYEPIYSQIATRNEYTEAEPS 1493 F SF M+ KCY FI N ISIFSSSYLYAEY KTFR M+R NISFR F FLLYA D+DG ++L+E N Y K+I NFNG L S+ ++ + HF ++ F++N L +D+ G I DIF MK FPD+F NYPVP +++ + NK YFWIGN+G Y G G YE FGC++ LY+D ++ I ++ FS++C + E +CVKDNCS+ NFLDCSF +NT RMCYKDLIR DNQSV+YKKFPDSNESTSFKIIWKQERFE+FCL EI+M +DGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFKLICPSKIPS PHKIYFFLSLIYDAMMTFLFAVLCTLRFCSNSKQKVVKITSPIEINNNETTYVEETGDYEPIYSQIATRNE TEAEPS Sbjct: 3 FVSFAMEYLKCYLFISHLSNFISIFSSSYLYAEYGKTFRNMKRFSINISFRGFDGFLLYAIDTDGNRMLVEMENFYGRKQISLNFNGILHKISVIYHTDSVYHFAQIMRFETNKLLFDQ-GAIYDIFPMKRVFPDSFENYPVPNNYSRIANKTYFWIGNVGPYTGMNGIKYEALRDAIFGCIHFLYIDFKKIQIINNSKNFSKSCTNSYRETICVKDNCSVQNKSENFLDCSFSGLNTNRMCYKDLIRNDNQSVVYKKFPDSNESTSFKIIWKQERFERFCLAEIDM---------------KDGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFKLICPSKIPSIPHKIYFFLSLIYDAMMTFLFAVLCTLRFCSNSKQKVVKITSPIEINNNETTYVEETGDYEPIYSQIATRNENTEAEPS 482
BLAST of SMED30017519 vs. Planmine SMEST
Match: SMESG000055195.1 (SMESG000055195.1) HSP 1 Score: 679.093 bits (1751), Expect = 0.000e+0 Identity = 349/491 (71.08%), Postives = 389/491 (79.23%), Query Frame = 3 Query: 21 MDNFKCYFFILGFVNLISIFSSSYLYAEYNKTFRKMERIVFNISFRDFGVFLLYATDSDGFKLLIEAGNLYKTKEIYFNFNGHLFNKSITNFSNFINHFDVVLTFQSNSLKYDENGVISDIFYMKPFFPDAFRNYPVPKDFTNLKNKVYFWIGNIGRYVGFKGRIYEPFTTPTFGCLNSLYVDDYSLEFIKEAEYFSRTCLHLVDEHLCVKDNCSLIKTKVNFLDCSFPSVNTKRMCYKDLIRTDNQSVIYKKFPDSNESTSFKIIWKQERFEKFCLTEIEMXXXXXXXXXXXXXXXRDGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFKLICPSKIPSTPHKIYFFLSLIYDAMMTFLFAVLCTLRFCSNSKQKVVKITSPIEINNNETTYVEETGDYEPIYSQIATRNEYTEAEPS 1493 M+ KCY FI N ISIFSSSYLYAEY KTFR M+R NISFR F FLLYA D+DG ++L+E N Y K+I NFNG L S+ ++ + HF ++ F++N L +D+ G I DIF MK FPD+F NYPVP +++ + NK YFWIGN+G Y G G YE FGC++ LY+D ++ I ++ FS++C + E +CVKDNCS+ NFLDCSF +NT RMCYKDLIR DNQSV+YKKFPDSNESTSFKIIWKQERFE+FCL EI+M +DGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFKLICPSKIPS PHKIYFFLSLIYDAMMTFLFAVLCTLRFCSNSKQKVVKITSPIEINNNETTYVEETGDYEPIYSQIATRNE TEAEPS Sbjct: 1 MEYLKCYLFISHLSNFISIFSSSYLYAEYGKTFRNMKRFSINISFRGFDGFLLYAIDTDGNRMLVEMENFYGRKQISLNFNGILHKISVIYHTDSVYHFAQIMRFETNKLLFDQ-GAIYDIFPMKRVFPDSFENYPVPNNYSRIANKTYFWIGNVGPYTGMNGIKYEALRDAIFGCIHFLYIDFKKIQIINNSKNFSKSCTNSYRETICVKDNCSVQNKSENFLDCSFSGLNTNRMCYKDLIRNDNQSVVYKKFPDSNESTSFKIIWKQERFERFCLAEIDM---------------KDGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFKLICPSKIPSIPHKIYFFLSLIYDAMMTFLFAVLCTLRFCSNSKQKVVKITSPIEINNNETTYVEETGDYEPIYSQIATRNENTEAEPS 475
BLAST of SMED30017519 vs. Planmine SMEST
Match: SMESG000055206.1 (SMESG000055206.1) HSP 1 Score: 657.522 bits (1695), Expect = 0.000e+0 Identity = 344/455 (75.60%), Postives = 361/455 (79.34%), Query Frame = 3 Query: 126 MERIVFNISFRDFGVFLLYATDSDGFKLLIEAGNLYKTKEIYFNFNGHLFNKSITNFSNFINHFDVVLTFQSNSLKYDENGVISDIFYMKPFFPDAFRNYPVPKDFTNLKNKVYFWIGNIGRYVGFKGRIYEPFTTPTFGCLNSLYVDDYSLEFIKEAEYFSRTCLHLVDEHLCVKDNCSLIKTKVNFLDCSFPSVNTKRMCYKDLIRTDNQSVIYKKFPDSNESTSFKIIWKQERFEKFCLTEIEMXXXXXXXXXXXXXXXRDGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFKLICPSKIPSTPHKIYFFLSLIYDAMMTFLFAVLCTLRFCSNSKQKVVKITSPIEINNNETTYVEETGDYEPIYSQIATRNEYTEAEP 1490 MERIVFNISFRDFG FLLYATDSDGFKLLIEAGNLYKTKEIYFNFNGHLFNKSITNFSNFINHFDVVLTFQSNSLKYD+NGVISDIFYMKPFF DAFRNYPVPKDFTNLKNKVYFWIGNIGRYVGF+GRIYEPFTTPTFGCLNSLYVDDYSLEFIKEAEYFSRTCLHLVDEHLCVKDNCSLIKTK NFLDCSFPSVNTKRMCYKDLIR DNQSV+YKKF DSNESTSFKIIWKQERFE+FCL EI+M +DGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSC Y+FLS Y +T L + F S +++ SP NN E+ V+ + P+ I + E EP Sbjct: 1 MERIVFNISFRDFGGFLLYATDSDGFKLLIEAGNLYKTKEIYFNFNGHLFNKSITNFSNFINHFDVVLTFQSNSLKYDKNGVISDIFYMKPFFSDAFRNYPVPKDFTNLKNKVYFWIGNIGRYVGFEGRIYEPFTTPTFGCLNSLYVDDYSLEFIKEAEYFSRTCLHLVDEHLCVKDNCSLIKTKENFLDCSFPSVNTKRMCYKDLIRNDNQSVVYKKFLDSNESTSFKIIWKQERFERFCLAEIDM---------------KDGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSC------------------------------FYYFLSFGYSFFITLLCGASFLIYFKSKFRRQNPTFESPYNGNNYESVEVQNMA-FRPL--PIPEKEEKNLTEP 407
BLAST of SMED30017519 vs. Planmine SMEST
Match: SMESG000055117.1 (SMESG000055117.1) HSP 1 Score: 175.637 bits (444), Expect = 6.895e-49 Identity = 134/490 (27.35%), Postives = 229/490 (46.73%), Query Frame = 3 Query: 27 NFKCYFFILGFVNLISIFSSSYLYAEYNKTFRKMERIVFNISFRDFGVFLLYATDSDGFKLLIEAGNLYKTK-EIYFNFNGHLFNKSITNFS-NFINHFDVVLTFQSNSL--KYDENGVISDIFYMKPFFPDAFRNYPVPKDFTNLKNKVYFWIGNIGRYVGFKGRIYEPFTTPTFGCLNSLYVDDYSLEFIKEAEYFSRTCLHLVDEHLCVKDNCSLIKTKVNFLDCSFPSVNTKRMCYKDLIRTDNQSVIYKKFPDSNESTSFKIIWKQERFEKFCLTEIEMXXXXXXXXXXXXXXXRDGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFK---LICPSKIPSTPH-----KIYFFLSLIYDAMMTFLFAVLCTL-----RFCSNSKQKVVKITSPIEINNNETTYVEETGDYEPI 1445 NF+ Y +L I++ S+LY E + F + F ++ +DF LLYA D++G L I+ +L+ K E +NFNG F K + + + + ++L F+ N+L KY+ +SDIF D F +P+P + ++KNK + WIGN+ Y+ + GCL+ Y++ ++F + + FS C+ DE++C ++C K N LDCSF S+N C+KDL +NQ +Y+ + E +SFK+IW+ FCL + + D S FY+ N + + +++L I++++ +++++ +KI+NG + +F+D +K I +G S C F G I YD +QF+ L +K S+ + I LS ++ L +CT+ +F S + + + E+ N TTY E+ YE I Sbjct: 2 NFQVYLIMLMLNFQINLSRESHLYQEIDGEFGNKISLSFGVTLKDFNGVLLYAIDTNGNWLSIKTRDLFYLKNEFVYNFNGQKFIKPSDSVNVHSVLQMHILLKFKENNLYFKYEPEYSLSDIFNKSHLHEDYFDKFPMPHRYLSVKNKTFIWIGNVPSG-------YKTYVKSNIGCLDYFYINSLRVKFDQIGKEFSPFCIEEKDENICYNNSCYSNKFFENILDCSFFSLNVNSHCHKDLSDHENQRNLYENLTSNVEESSFKLIWRFNYTNNFCLLNVILS---------------DNSTIRFYMDNENTVRMRLTGRNSKTISLQRTL---RAGSINIIIIKCEDTELYVKITNGSSVTSFRDYISSTVKLIYLGVSTGMTDCTDFNGHIDLLYDATQFQRRALPIINKCTSSNYLKENTWIILLLSSGVINLVLILAVSVCTIKWKVRKFSSLKSRNSYETVNHSELPNVVTTYQLESVGYETI 466
BLAST of SMED30017519 vs. Planmine SMEST
Match: SMESG000056437.1 (SMESG000056437.1) HSP 1 Score: 170.629 bits (431), Expect = 6.458e-47 Identity = 122/471 (25.90%), Postives = 219/471 (46.50%), Query Frame = 3 Query: 18 LMDNFKCYFFILGFVNLISIFSSSYLYAEYNKTFRKMERIVFNISFRDFG-VFLLYATDSDGFKLLIEAGNLYKTK-EIYFNFNGHLFNKSIT--NFSNFINHFDVVLTFQSNSLKYDE----NGVISDIFYMKPFFPDAFRNYPVPKDFTNLKNKVYFWIGNIGRYVGFKGRIYEPFTTPTFGCLNSLYVDDYSLEFIKEAEYFSRTCLHLVDEHLCVKDNCSLIKTKVNFLDCSFPSVNTKRMCYKDLIRTDNQSVIYKKFPDSNESTSFKIIWKQERFEKFCLTEIEMXXXXXXXXXXXXXXXRDGSYFIFYITENNELSKCFIENRFSPVCTKKSLFNVTTDEIHVLMFTFDNSQVLIKISNGKYIQTFKDEFQRKIKQIKIGYSENTKSCVRFQGEITFRYDISQFKLICPSK--------IPSTPHKIYFFLSLIYDAMMTFLFAV-LCTLRFCSNSKQKVVKIT 1379 +M +FK + F+N I +FSS YLY + ++ F + ++ FRD LLYA D + + LIE+ Y+ + +I FNFNG +FN + T FS + F + L F+ ++L + + + ++F PD F YP+ D + FW+GN+G +G +++P GC+ + ++ ++F + C E +C+K C + + LDCS +NTK CY+D + Q++ Y E K++WKQE + +CL EIE + S + Y NE+ NR S T+++L + + IHVL+ F ++KISNG + TF + + I +I + Y + C F+G++ F+Y+ F + + I + + ++++ ++ + + +C L+ NS++K+ +T Sbjct: 1 MMLSFKRCLLLSFFINYIQLFSSGYLYGKLDENFENGMQFYIDLDFRDVSHGILLYAIDRNRNRFLIESAEKYRGRRKIVFNFNGQIFNSTNTYWRFSG-TSTFTIFLQFKYDNLYIMDYSYRHATLLNVFNRNKTNPDTFVGYPISDDHNSTTRTTDFWLGNVGPSIGKDKTVFQPSKVQMVGCVYNFRINYKEIKFTTYGDDLLPFCRKQFSETICIKGKCKKEQRRSYILDCSLSEMNTKEYCYEDFLTYMGQTLSYLTVGLGEEPRIMKMVWKQEFSKDYCLLEIE---------------TKKESSILVYANRLNEIVLNIRSNR-SVEWTRRTLCKMQPNLIHVLLIEFVYPNFVLKISNGSNVLTFIEHYPDYIIKIDVAYKSHRNLCSTFEGQLAFQYEPGLFLQLNKQETTAVNVFHIIHSWRSVLCLITILSLGLVILVIGICICHLKKVENSRKKLTIVT 454 The following BLAST results are available for this feature:
BLAST of SMED30017519 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30017519 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30017519 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30017519 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30017519 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30017519 ID=SMED30017519|Name=SMED30017519|organism=Schmidtea mediterranea sexual|type=transcript|length=1579bpback to top protein sequence of SMED30017519-orf-1 >SMED30017519-orf-1 ID=SMED30017519-orf-1|Name=SMED30017519-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=499bp MDNFKCYFFILGFVNLISIFSSSYLYAEYNKTFRKMERIVFNISFRDFGVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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