Spermatogenesis associated 1

Overview
NameSpermatogenesis associated 1
Smed IDSMED30017491
Length (bp)867
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Spermatogenesis associated 1 (SMED30017491) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Spermatogenesis associated 1 (SMED30017491) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017491

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30017491h1SMcG0000907 SmedASXL_008906SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30017491h1SMcG0000907 dd_Smed_v4_13541_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30017491h1SMcG0000907 dd_Smed_v4_13541_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30017491h1SMcG0000907 dd_Smed_v4_13541_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Spermatogenesis associated 1 vs. Ensembl Xenopus
Match: SPATA1 (spermatogenesis associated 1 [Source:NCBI gene;Acc:100498562])

HSP 1 Score: 57.3806 bits (137), Expect = 1.008e-8
Identity = 50/162 (30.86%), Postives = 94/162 (58.02%), Query Frame = 2 
Query:  197 KELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQ-DIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAI 679
            ++L  +  +R  MEK RE+L+RK   L+ + K  R ++ + WKK+++E+KK+T  +E+ +N+ R +LE  + K++  + A  RD++  K    P L   S    N V  +I  KQ ++++++R V+  + KL  EIK+  QA  E   ++A+  ++  Q ++
Sbjct:  277 QQLNQLKDERMQMEKAREELVRKAKVLIEQYKLKRHQARDSWKKKYFERKKVTSVLEETLNKQRNDLEVYYKKLMIQLAA--RDSR--KRSKAPALAANSK---NAVIISITTKQHELDQLKRKVENARIKLLIEIKMRKQASSELNVLKAELAQKKAQSSL 431
[Close]
BLAST of Spermatogenesis associated 1 vs. Ensembl Mouse
Match: Spata1 (spermatogenesis associated 1 [Source:MGI Symbol;Acc:MGI:1918201])

HSP 1 Score: 50.8322 bits (120), Expect = 8.834e-7
Identity = 41/157 (26.11%), Postives = 95/157 (60.51%), Query Frame = 2 
Query:  200 ELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPEL-DKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNI 667
            +++ + ++R  +E IRE+L++K+  L  + K  R  +++ WKK++ + KK+T  +E+ + +LR +LE  + K+  +M+   R+ K ++   L  + D ++Y    ++ +  +++  I++++R +D  K KL  E+K+  QA  + + ++AD T++ +
Sbjct:  280 QMKQVKEERKYLENIREELIKKVDKLFEQNKSKRYHASDSWKKKYLDTKKVTASLEEVLTKLREDLELYYKKL--LMQLEAREIK-MRPRNLANISDSKNY----LIIQITEVQHAIDQLKRKLDTDKMKLILEVKMRKQAVSDLQTLKADLTQKKM 429
[Close]
BLAST of Spermatogenesis associated 1 vs. Ensembl Mouse
Match: Spata1 (spermatogenesis associated 1 [Source:MGI Symbol;Acc:MGI:1918201])

HSP 1 Score: 50.8322 bits (120), Expect = 8.834e-7
Identity = 41/157 (26.11%), Postives = 95/157 (60.51%), Query Frame = 2 
Query:  200 ELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPEL-DKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNI 667
            +++ + ++R  +E IRE+L++K+  L  + K  R  +++ WKK++ + KK+T  +E+ + +LR +LE  + K+  +M+   R+ K ++   L  + D ++Y    ++ +  +++  I++++R +D  K KL  E+K+  QA  + + ++AD T++ +
Sbjct:  280 QMKQVKEERKYLENIREELIKKVDKLFEQNKSKRYHASDSWKKKYLDTKKVTASLEEVLTKLREDLELYYKKL--LMQLEAREIK-MRPRNLANISDSKNY----LIIQITEVQHAIDQLKRKLDTDKMKLILEVKMRKQAVSDLQTLKADLTQKKM 429
[Close]
BLAST of Spermatogenesis associated 1 vs. UniProt/SwissProt
Match: sp|Q6AY22|SPAT1_RAT (Spermatogenesis-associated protein 1 OS=Rattus norvegicus OX=10116 GN=Spata1 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 1.759e-6
Identity = 41/157 (26.11%), Postives = 95/157 (60.51%), Query Frame = 2 
Query:  200 ELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPEL-DKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNI 667
            +++ + ++R  +E IRE+L++K+  L  + K  R  + + WKK++++ KK+T  +E+ + +LR +LE  + K+  +M+   R+ K ++   L  + D ++Y    ++ +  +++  I++++R +D  K KL  E+K+  QA  + + ++AD T++ +
Sbjct:  280 QMKQVKEERKYLENIREELIKKVEKLFEQSKSKRYHACDSWKKKYFDTKKVTASLEEVLTKLREDLELYYKKL--LMQLEAREIK-MRPRNLANISDSKNY----LIIQITEVQHAIDQLKRKLDTDKMKLILEVKMRKQAASDLQTLRADLTQKKM 429
[Close]
BLAST of Spermatogenesis associated 1 vs. UniProt/SwissProt
Match: sp|Q9D5R4|SPAT1_MOUSE (Spermatogenesis-associated protein 1 OS=Mus musculus OX=10090 GN=Spata1 PE=2 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 6.179e-6
Identity = 41/157 (26.11%), Postives = 95/157 (60.51%), Query Frame = 2 
Query:  200 ELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPEL-DKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNI 667
            +++ + ++R  +E IRE+L++K+  L  + K  R  +++ WKK++ + KK+T  +E+ + +LR +LE  + K+  +M+   R+ K ++   L  + D ++Y    ++ +  +++  I++++R +D  K KL  E+K+  QA  + + ++AD T++ +
Sbjct:  280 QMKQVKEERKYLENIREELIKKVDKLFEQNKSKRYHASDSWKKKYLDTKKVTASLEEVLTKLREDLELYYKKL--LMQLEAREIK-MRPRNLANISDSKNY----LIIQITEVQHAIDQLKRKLDTDKMKLILEVKMRKQAVSDLQTLKADLTQKKM 429
[Close]
BLAST of Spermatogenesis associated 1 vs. TrEMBL
Match: A0A267F575 (SPATA1_C domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005998g1 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 7.156e-25
Identity = 66/177 (37.29%), Postives = 116/177 (65.54%), Query Frame = 2 
Query:  170 DGDQLFRKPKELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELA 700
            D +QL     EL+ I  DR  +E+ R+ L+R+   L A+ + +R ++ + WKKR++E+KKLT P+E + N LR  LE+ H ++V+ M +R+++ K   ++  P+    +  QLN+  +A +++ +IEE+QR VD+ K KLTAE+KL  QAE E R ++A+ T+R + L++ R++++ 
Sbjct:   22 DREQLL---SELQRIRDDRTEIERRRDDLIRRARQLQARTQNMRNQARDMWKKRYFEEKKLTTPLEDQANRLRDELESYHKRMVSTMTSRVKEGK---NIDTPD----ATQQLNLRIQATRMQHEIEELQRRVDDSKMKLTAEVKLRTQAEAELRAMKAELTQRKLNLSMTRTQQMG 188
[Close]
BLAST of Spermatogenesis associated 1 vs. TrEMBL
Match: A0A267F5C1 (SPATA1_C domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024004g1 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 7.232e-25
Identity = 66/177 (37.29%), Postives = 116/177 (65.54%), Query Frame = 2 
Query:  170 DGDQLFRKPKELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELA 700
            D +QL     EL+ I  DR  +E+ R+ L+R+   L A+ + +R ++ + WKKR++E+KKLT P+E + N LR  LE+ H ++V+ M +R+++ K   ++  P+    +  QLN+  +A +++ +IEE+QR VD+ K KLTAE+KL  QAE E R ++A+ T+R + L++ R++++ 
Sbjct:   22 DREQLL---SELQRIRDDRTEIERRRDDLIRRARQLQARTQNMRNQARDMWKKRYFEEKKLTTPLEDQANRLRDELESYHKRMVSTMTSRVKEGK---NIDTPD----ATQQLNLRIQATRMQHEIEELQRRVDDSKMKLTAEVKLRSQAEAELRAMKAELTQRKLNLSMTRTQQMG 188
[Close]
BLAST of Spermatogenesis associated 1 vs. TrEMBL
Match: A0A1I8I535 (SPATA1_C domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.137e-23
Identity = 63/167 (37.72%), Postives = 112/167 (67.07%), Query Frame = 2 
Query:  200 ELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELA 700
            EL+ I  DR  +E+ R+ L+R+   L A+ + +R ++ + WKKR++E+KKLT P+E + N LR  LE+ H ++V+ M +R+++ K   ++  P+    +  QLN+  +A +++ +IEE+QR VD+ K KLTAE+KL  QAE E R ++A+ T+R + L++ R++++ 
Sbjct:  190 ELQRIRDDRTEIERRRDDLIRRARQLQARTQNMRNQARDMWKKRYFEEKKLTTPLEDQANRLRDELESYHKRMVSTMTSRVKEGK---NIDTPD----ATQQLNLRIQATRMQHEIEELQRRVDDSKMKLTAEVKLRTQAEAELRAMKAELTQRKLNLSMTRTQQMG 349
[Close]
BLAST of Spermatogenesis associated 1 vs. TrEMBL
Match: A0A267FRY9 (SPATA1_C domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003676g2 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 3.099e-21
Identity = 61/167 (36.53%), Postives = 110/167 (65.87%), Query Frame = 2 
Query:  203 LEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELAK 703
            L+ I  DR  +E+ RE L+R+   L ++++ LR ++ + WKKR++E KK T P+E++ N+LR  LEA H ++V  M +R+++ + +          ++  QLN+  +A +++ +IEE+QR VD+ K KLTAE+KL  QAE E R ++++ T+R + L++ RS+++ +
Sbjct:  335 LQKIRDDRLEIERRREDLMRRAKQLQSRMQNLRNQARDMWKKRYFEAKKQTVPLEEQTNKLREELEAYHKRMVGTMTSRVKEGRNVDA-------PDATQQLNLRIQATRMQHEIEELQRRVDDSKMKLTAEVKLRTQAESELRTMKSELTQRKLNLSVTRSQQMGQ 494
[Close]
BLAST of Spermatogenesis associated 1 vs. TrEMBL
Match: A0A267GYE7 (SPATA1_C domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003676g1 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 3.605e-21
Identity = 61/167 (36.53%), Postives = 110/167 (65.87%), Query Frame = 2 
Query:  203 LEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELAK 703
            L+ I  DR  +E+ RE L+R+   L ++++ LR ++ + WKKR++E KK T P+E++ N+LR  LEA H ++V  M +R+++ + +          ++  QLN+  +A +++ +IEE+QR VD+ K KLTAE+KL  QAE E R ++++ T+R + L++ RS+++ +
Sbjct:  340 LQKIRDDRLEIERRREDLMRRAKQLQSRMQNLRNQARDMWKKRYFEAKKQTVPLEEQTNKLREELEAYHKRMVGTMTSRVKEARNVDA-------PDATQQLNLRIQATRMQHEIEELQRRVDDSKMKLTAEVKLRTQAESELRTMKSELTQRKLNLSVTRSQQMGQ 499
[Close]
BLAST of Spermatogenesis associated 1 vs. Ensembl Cavefish
Match: si:dkey-205k8.5 (spermatogenesis associated 1 [Source:NCBI gene;Acc:103031330])

HSP 1 Score: 58.151 bits (139), Expect = 2.594e-9
Identity = 50/169 (29.59%), Postives = 93/169 (55.03%), Query Frame = 2 
Query:  173 GDQLFRKPKELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAI 679
            G++L    KEL  + ++R  +EK R++LLRK   LLA  +  R ++ + WK+++++ KK T P+E+ +  LR  LE  + K+   ++AR    +  +  G P   K      N++ + +    +I+ +++ +D+ K KL  E KL  QA  E R ++A+  ++  Q +I
Sbjct:  217 GNELL---KELALVKEERKQLEKTRQELLRKGKELLALNRHRRNQARDKWKRKYFDTKKATAPLEEALKSLRQELETFYDKLFQQLQARDGRNRQ-RRTGNPSSTKN-----NLIIQIMTESWEIDNLKKNLDDAKMKLVTETKLKKQAATELRALKAELMQKKSQCSI 376
[Close]
BLAST of Spermatogenesis associated 1 vs. Ensembl Cavefish
Match: si:dkey-205k8.5 (spermatogenesis associated 1 [Source:NCBI gene;Acc:103031330])

HSP 1 Score: 58.151 bits (139), Expect = 3.047e-9
Identity = 51/177 (28.81%), Postives = 96/177 (54.24%), Query Frame = 2 
Query:  149 KCQITIEDGDQLFRKPKELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAI 679
            K Q  + + ++L    KEL  + ++R  +EK R++LLRK   LLA  +  R ++ + WK+++++ KK T P+E+ +  LR  LE  + K+   ++AR    +  +  G P   K      N++ + +    +I+ +++ +D+ K KL  E KL  QA  E R ++A+  ++  Q +I
Sbjct:  311 KSQTALTNRNELL---KELALVKEERKQLEKTRQELLRKGKELLALNRHRRNQARDKWKRKYFDTKKATAPLEEALKSLRQELETFYDKLFQQLQARDGRNRQ-RRTGNPSSTKN-----NLIIQIMTESWEIDNLKKNLDDAKMKLVTETKLKKQAATELRALKAELMQKKSQCSI 478
[Close]
BLAST of Spermatogenesis associated 1 vs. Ensembl Nematostella
Match: EDO46192 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN1])

HSP 1 Score: 66.2402 bits (160), Expect = 3.121e-12
Identity = 51/171 (29.82%), Postives = 95/171 (55.56%), Query Frame = 2 
Query:  200 ELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRS-KELAKMG 709
            EL     +R ++EK RE+ +R+   L  +  + R +S   WKK+F+++K+ T P+E+++N LR   E  H  +++ ++ +       K  G  ELDK    + N +    +L+ ++E+++R V+E K  L +E+KL   A+ E + ++ D   + I L + +S K LA +G
Sbjct:  583 ELRRAKNERVDLEKQREQKVRRAKTLQTQTMQKRNQSKNIWKKKFFDEKRKTAPLEEQVNRLRYETEVQHQAMLSFLETKKDREGGAK--GGSELDKTPSQKSNQLILLARLQNEVEDLRRKVEETKMMLASEMKLRDAAQNELKQLREDLLDKKINLTLTKSQKSLATLG 751
[Close]
BLAST of Spermatogenesis associated 1 vs. Ensembl Medaka
Match: si:dkey-205k8.5 (spermatogenesis associated 1 [Source:NCBI gene;Acc:101173620])

HSP 1 Score: 47.3654 bits (111), Expect = 9.353e-6
Identity = 45/136 (33.09%), Postives = 78/136 (57.35%), Query Frame = 2 
Query:  200 ELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKL 607
            E++ + + R  +E  +++LLRK   LLA+    R ++   WKK+F+E KK T P+E+ +  LR  +E  ++K+V  ++AR    K ++  G   L  E    L V+   +K   +I+ ++R V++ + KL AEIKL
Sbjct:  340 EIKLVREKRKQLEWTKQELLRKGKDLLAQSSHRRNQARNCWKKKFFETKKATGPLEETLKNLRQEMETFYNKLVLQLQARESRGK-MRRQGRSSLKNE----LTVLI--MKETYEIDNLKRKVEDAEMKLVAEIKL 468
[Close]
BLAST of Spermatogenesis associated 1 vs. Ensembl Medaka
Match: si:dkey-205k8.5 (spermatogenesis associated 1 [Source:NCBI gene;Acc:101173620])

HSP 1 Score: 47.3654 bits (111), Expect = 9.735e-6
Identity = 45/136 (33.09%), Postives = 78/136 (57.35%), Query Frame = 2 
Query:  200 ELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKL 607
            E++ + + R  +E  +++LLRK   LLA+    R ++   WKK+F+E KK T P+E+ +  LR  +E  ++K+V  ++AR    K ++  G   L  E    L V+   +K   +I+ ++R V++ + KL AEIKL
Sbjct:  345 EIKLVREKRKQLEWTKQELLRKGKDLLAQSSHRRNQARNCWKKKFFETKKATGPLEETLKNLRQEMETFYNKLVLQLQARESRGK-MRRQGRSSLKNE----LTVLI--MKETYEIDNLKRKVEDAEMKLVAEIKL 473
[Close]
BLAST of Spermatogenesis associated 1 vs. Planmine SMEST
Match: SMESG000029165.1 (SMESG000029165.1)

HSP 1 Score: 408.683 bits (1049), Expect = 4.574e-144
Identity = 212/213 (99.53%), Postives = 213/213 (100.00%), Query Frame = 2 
Query:  110 RNSQSRNHGNSGNKCQITIEDGDQLFRKPKELEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELAKMGTRDYHAHIQTSFH 748
            +NSQSRNHGNSGNKCQITIEDGDQLFRKPKELEDIIKDRDNMEKIREKLLRKMFYLLAKLKKLRAKSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELAKMGTRDYHAHIQTSFH
Sbjct:  132 KNSQSRNHGNSGNKCQITIEDGDQLFRKPKELEDIIKDRDNMEKIREKLLRKMFYLLAKLKKLRAKSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELAKMGTRDYHAHIQTSFH 344
[Close]
BLAST of Spermatogenesis associated 1 vs. Planmine SMEST
Match: SMESG000004654.1 (SMESG000004654.1)

HSP 1 Score: 93.5893 bits (231), Expect = 2.416e-21
Identity = 63/181 (34.81%), Postives = 102/181 (56.35%), Query Frame = 2 
Query:  221 DRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTK------WIKDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELAKMGTRDYHAHIQTSF 745
            +R + E  R+ L+RK   L  K    +  + + WKKR++E+KK  PP+E  +N+ R+ L+A H KI++ M++ ++D +       I D+G             +  +A KL+ +IEE QR VDE K KLT EIK+  QA  E   ++AD  ++ I L++ RS+ L+ MGT+D +   + S 
Sbjct:  316 ERMDTESRRDDLIRKAKQLYTKTANQKNIARDMWKKRYFEEKKRNPPMEDHLNKQRSELDAIHRKIMSTMESFMKDNRNRGANEKIVDVG------------KMRMQAKKLEYEIEEAQRGVDEAKLKLTCEIKMTHQARAEVVAMRADLVQKKINLSMARSQHLSSMGTQDVYRSTRVSL 484
[Close]
BLAST of Spermatogenesis associated 1 vs. Planmine SMEST
Match: SMESG000049418.1 (SMESG000049418.1)

HSP 1 Score: 75.0998 bits (183), Expect = 6.558e-15
Identity = 49/171 (28.65%), Postives = 96/171 (56.14%), Query Frame = 2 
Query:  203 LEDIIKDRDNMEKIREKLLRKMFYXXXXXXXXXXXSNEDWKKRFYEQKKLTPPIEKKMNELRANLEATHSKIVNIMKARLRDTKWI-KDLGLPELDKESYDQLNVVTRAIKLKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAIIRSKELAKMGT 712
            L+  I +R   E  RE+ +RK+      +   R +  + WKKR++E+KK TPP+E ++   R  +E  H K++  M++ LR+ + + K  G+        ++ N++ +A +++ +IE +QR ++  + +L+AE+KL  Q E E + ++ +  +  I+L+  RS +L  M  
Sbjct:  570 LKSAIAERQQSESRREEAIRKIRASQMNVHTKRNQIRDFWKKRYFEEKKKTPPLEDQLARYREEIEILHKKLLGFMESGLREHRKLQKRNGI----MSPSNKANLMLQAARIQHEIENLQRQIEHCQMRLSAEMKLRSQTETELKVLRIEAMQNKIRLSRTRSDQLVIMAA 736
The following BLAST results are available for this feature:
BLAST of Spermatogenesis associated 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SPATA11.008e-830.86spermatogenesis associated 1 [Source:NCBI gene;Acc... [more]
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BLAST of Spermatogenesis associated 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Spata18.834e-726.11spermatogenesis associated 1 [Source:MGI Symbol;Ac... [more]
Spata18.834e-726.11spermatogenesis associated 1 [Source:MGI Symbol;Ac... [more]
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BLAST of Spermatogenesis associated 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q6AY22|SPAT1_RAT1.759e-626.11Spermatogenesis-associated protein 1 OS=Rattus nor... [more]
sp|Q9D5R4|SPAT1_MOUSE6.179e-626.11Spermatogenesis-associated protein 1 OS=Mus muscul... [more]
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BLAST of Spermatogenesis associated 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267F5757.156e-2537.29SPATA1_C domain-containing protein OS=Macrostomum ... [more]
A0A267F5C17.232e-2537.29SPATA1_C domain-containing protein OS=Macrostomum ... [more]
A0A1I8I5352.137e-2337.72SPATA1_C domain-containing protein OS=Macrostomum ... [more]
A0A267FRY93.099e-2136.53SPATA1_C domain-containing protein (Fragment) OS=M... [more]
A0A267GYE73.605e-2136.53SPATA1_C domain-containing protein (Fragment) OS=M... [more]
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BLAST of Spermatogenesis associated 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:dkey-205k8.52.594e-929.59spermatogenesis associated 1 [Source:NCBI gene;Acc... [more]
si:dkey-205k8.53.047e-928.81spermatogenesis associated 1 [Source:NCBI gene;Acc... [more]
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BLAST of Spermatogenesis associated 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spermatogenesis associated 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO461923.121e-1229.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Spermatogenesis associated 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:dkey-205k8.59.353e-633.09spermatogenesis associated 1 [Source:NCBI gene;Acc... [more]
si:dkey-205k8.59.735e-633.09spermatogenesis associated 1 [Source:NCBI gene;Acc... [more]
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BLAST of Spermatogenesis associated 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000029165.14.574e-14499.53SMESG000029165.1[more]
SMESG000004654.12.416e-2134.81SMESG000004654.1[more]
SMESG000049418.16.558e-1528.65SMESG000049418.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017491 ID=SMED30017491|Name=Spermatogenesis associated 1|organism=Schmidtea mediterranea sexual|type=transcript|length=867bp
GTGGATAAGCGGGGTTCACTGTACCATCTTGATGATTTAGTATTCGAATT
CGGTTCAATCATTTATTTACCACAAATTACAAATGAGTGTTAAAATTGTT
TTATTTCAAAGAAATTCCCAATCTCGAAATCATGGAAATTCTGGAAACAA
ATGCCAAATAACTATTGAAGATGGCGATCAATTATTTAGAAAACCAAAAG
AACTTGAAGACATAATAAAAGACAGGGATAACATGGAGAAAATACGAGAA
AAACTTTTAAGGAAAATGTTTTATTTGTTGGCTAAATTAAAGAAATTAAG
AGCTAAAAGTAACGAAGATTGGAAGAAACGATTTTATGAACAAAAAAAAC
TTACTCCACCAATAGAAAAGAAAATGAATGAGTTACGGGCCAATTTAGAA
GCGACTCATTCGAAAATAGTCAATATTATGAAAGCAAGACTCAGAGACAC
CAAATGGATTAAAGATCTTGGATTGCCAGAACTTGATAAAGAATCTTATG
ATCAGTTGAATGTGGTCACTCGAGCAATAAAACTCAAACAAGATATAGAA
GAAATCCAAAGAATTGTAGATGAAGGAAAAGAAAAGCTTACTGCTGAAAT
CAAGTTGCATTGCCAAGCAGAACAAGAAGCTCGGTTTATTCAAGCGGACA
CCACAAGACGAAATATTCAATTAGCAATTATAAGATCTAAAGAATTGGCC
AAAATGGGCACCCGAGATTACCATGCTCATATTCAAACATCTTTTCACTG
ATTTACTAGTATAAATGTTACTGTTACTAATTTACATTTATCTATTAAAA
AAAATATATATATAAATTTATATTTTTATTTTTTGCCTATTTTCGTCACC
TTTGATTTAAATTGTTT
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protein sequence of SMED30017491-orf-1

>SMED30017491-orf-1 ID=SMED30017491-orf-1|Name=SMED30017491-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=223bp
MSVKIVLFQRNSQSRNHGNSGNKCQITIEDGDQLFRKPKELEDIIKDRDN
MEKIREKLLRKMFYLLAKLKKLRAKSNEDWKKRFYEQKKLTPPIEKKMNE
LRANLEATHSKIVNIMKARLRDTKWIKDLGLPELDKESYDQLNVVTRAIK
LKQDIEEIQRIVDEGKEKLTAEIKLHCQAEQEARFIQADTTRRNIQLAII
RSKELAKMGTRDYHAHIQTSFH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR039062SPAT1
IPR031478SPATA1_C
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 41..61
NoneNo IPR availableCOILSCoilCoilcoord: 148..168
IPR031478Spermatogenesis-associated protein 1, C-terminal domainPFAMPF15743SPATA1_Ccoord: 40..189
e-value: 1.5E-19
score: 70.6
IPR039062Spermatogenesis-associated protein 1PANTHERPTHR14421SPERMATOGENESIS-ASSOCIATED PROTEIN 1coord: 39..202