SMED30017473

Overview
NameSMED30017473
Smed IDSMED30017473
Length (bp)2104
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30017473 (SMED30017473) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017473 (SMED30017473) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017473 (SMED30017473) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017473 (SMED30017473) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017473

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30017473h1SMcG0001443 SmedASXL_016751SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
zeta neoblastSMED30017473h1SMcG0001443 dd_Smed_v4_10617_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30017473 vs. Planmine SMEST
Match: SMESG000007496.1 (SMESG000007496.1)

HSP 1 Score: 764.222 bits (1972), Expect = 0.000e+0
Identity = 462/589 (78.44%), Postives = 497/589 (84.38%), Query Frame = 1 
Query:   55 YIFRH--TRCQTGADINKPLSLLEKVEHQAAKR*RSYSRRPAKKPESVETLINTESKNEETGLKXXXXXXXXXXXXXXXXRE*TDQEKNGILREPKVNSRINMTENDTTTPITNSNEMTSQPERVDIGELREKLRGEPFDGSIDKLQTFIKELVVYTTICHWPDIVIRERLPLYLTGSV*DVYLEAKRDPSKLKTWNEIK*FLINTLENAKELNRKMLEFLHRRQKQNE*NVLFACELKRFAREAFGEEKVPVERIVLVFIKGLKHADIRAGMGCQAPQIVDKALEIANIC*KLRLPQQQVAAMTAGAVSTTLSSEFTNSQTTNINPTMNQFIPRSQQMFKVPQTRNPXXXXXXXXXXXXXXXXXXXXXXXPVCEIYQRIGHQRNE*FKAKTCQRCGKRGHTEVTCREFXXXXXXXXXXXXXXXXXXXCVNGEPKIVTCFTCNQVGHMSRNCPHQIQSXXXXXXXXXXXVQINLMQEVERLRNEIQQMKGNNQQQRIIMLKDNGDVREQIINVNQEKQRTEWQRLQMEEAKEIQARWERRNGTMKPINMMRAVNENEIHTAEQCVGNSDGDETSVNEDVLPENIIKCET 1815
            +IF H  TR QT AD NK  SLLE VEHQAAKR RSYSRRP+KKPESVET+INTES+NEETGLKLLEDILDN SD+E SDRE TDQE+N IL E KVNS INM E+ TTTPI NSNEMTSQPERVDIGE REK RGEPFDGSIDKLQTFIKE  VYTTICHWPDI IRERLPL LTGS  +VYLEAKRDP+KL+ WNEIK FLINT  NAKELNRK  EFLHR+QKQNE NVLFA ELKR AREAFG++K                 DIRAG+GCQAPQ VD+ALEIANIC   RLPQ QVAAMTAGAV+TT SSE  NSQTTN+NPTMNQFIPR+QQMFK P  RNP NNG GNLNYTNNFRFNNN NNG V E  QR GHQ ++ FK KTCQRCGKRGHT V CREFGN NVR+NG+G+NRGYGRN +NGE ++VTCFTCNQVGHMSRNCPH++QSNP  PN  N NVQINLMQE ERLRNEI+QMKG+NQQQRI ML++NGDVREQIINVNQE+QR EWQRLQMEEAKEIQARWERRNGT K INMMRAVNENE+HTA+QCV NSDGDETSVNEDVLPENIIKCET
Sbjct:  395 HIFIHLKTRSQTKADTNKNPSLLETVEHQAAKRSRSYSRRPSKKPESVETIINTESENEETGLKLLEDILDNPSDNEKSDRESTDQEENSILGESKVNSPINMAEDGTTTPIFNSNEMTSQPERVDIGEFREKFRGEPFDGSIDKLQTFIKEFEVYTTICHWPDIAIRERLPLCLTGSDQEVYLEAKRDPTKLRNWNEIKDFLINTFGNAKELNRKTFEFLHRKQKQNESNVLFAFELKRLAREAFGDKK-----------------DIRAGVGCQAPQTVDEALEIANICETFRLPQLQVAAMTAGAVTTTSSSEVNNSQTTNVNPTMNQFIPRAQQMFKAPPIRNPMNNGNGNLNYTNNFRFNNNENNGSVYENCQRFGHQSSDCFKTKTCQRCGKRGHTAVICREFGNGNVRFNGDGNNRGYGRNRINGESRMVTCFTCNQVGHMSRNCPHRMQSNPSEPNQTNANVQINLMQEFERLRNEIEQMKGSNQQQRINMLQENGDVREQIINVNQEQQRAEWQRLQMEEAKEIQARWERRNGTTKRINMMRAVNENEMHTADQCVVNSDGDETSVNEDVLPENIIKCET 966

HSP 2 Score: 70.0922 bits (170), Expect = 5.568e-12
Identity = 40/53 (75.47%), Postives = 44/53 (83.02%), Query Frame = 2 
Query: 1952 KPS*DDIATKNRIQRILLRKLEEKNITRK--TLTDEQIKQKLEKRWFTTKRWC 2104
            KPS   +ATKNRIQRILL+KLEEK I R+  TLTDEQ+KQKLEKRW T KRWC
Sbjct:  978 KPSKKAMATKNRIQRILLQKLEEK-IRREDETLTDEQVKQKLEKRWATAKRWC 1029
[Close]
BLAST of SMED30017473 vs. Planmine SMEST
Match: SMESG000007496.1 (SMESG000007496.1)

HSP 1 Score: 741.495 bits (1913), Expect = 0.000e+0
Identity = 451/578 (78.03%), Postives = 486/578 (84.08%), Query Frame = 1 
Query:   55 YIFRH--TRCQTGADINKPLSLLEKVEHQAAKR*RSYSRRPAKKPESVETLINTESKNEETGLKXXXXXXXXXXXXXXXXRE*TDQEKNGILREPKVNSRINMTENDTTTPITNSNEMTSQPERVDIGELREKLRGEPFDGSIDKLQTFIKELVVYTTICHWPDIVIRERLPLYLTGSV*DVYLEAKRDPSKLKTWNEIK*FLINTLENAKELNRKMLEFLHRRQKQNE*NVLFACELKRFAREAFGEEKVPVERIVLVFIKGLKHADIRAGMGCQAPQIVDKALEIANIC*KLRLPQQQVAAMTAGAVSTTLSSEFTNSQTTNINPTMNQFIPRSQQMFKVPQTRNPXXXXXXXXXXXXXXXXXXXXXXXPVCEIYQRIGHQRNE*FKAKTCQRCGKRGHTEVTCREFXXXXXXXXXXXXXXXXXXXCVNGEPKIVTCFTCNQVGHMSRNCPHQIQSXXXXXXXXXXXVQINLMQEVERLRNEIQQMKGNNQQQRIIMLKDNGDVREQIINVNQEKQRTEWQRLQMEEAKEIQARWERRNGTMKPINMMRAVNENEIHTAEQCVGNSDGDETSVNED 1782
            +IF H  TR QT AD NK  SLLE VEHQAAKR RSYSRRP+KKPESVET+INTES+NEETGLKLLEDILDN SD+E SDRE TDQE+N IL E KVNS INM E+ TTTPI NSNEMTSQPERVDIGE REK RGEPFDGSIDKLQTFIKE  VYTTICHWPDI IRERLPL LTGS  +VYLEAKRDP+KL+ WNEIK FLINT  NAKELNRK  EFLHR+QKQNE NVLFA ELKR AREAFG++K                 DIRAG+GCQAPQ VD+ALEIANIC   RLPQ QVAAMTAGAV+TT SSE  NSQTTN+NPTMNQFIPR+QQMFK P  RNP NNG GNLNYTNNFRFNNN NNG V E  QR GHQ ++ FK KTCQRCGKRGHT V CREFGN NVR+NG+G+NRGYGRN +NGE ++VTCFTCNQVGHMSRNCPH++QSNP  PN  N NVQINLMQE ERLRNEI+QMKG+NQQQRI ML++NGDVREQIINVNQE+QR EWQRLQMEEAKEIQARWERRNGT K INMMRAVNENE+HTA+QCV NSDGDETSVNED
Sbjct:  395 HIFIHLKTRSQTKADTNKNPSLLETVEHQAAKRSRSYSRRPSKKPESVETIINTESENEETGLKLLEDILDNPSDNEKSDRESTDQEENSILGESKVNSPINMAEDGTTTPIFNSNEMTSQPERVDIGEFREKFRGEPFDGSIDKLQTFIKEFEVYTTICHWPDIAIRERLPLCLTGSDQEVYLEAKRDPTKLRNWNEIKDFLINTFGNAKELNRKTFEFLHRKQKQNESNVLFAFELKRLAREAFGDKK-----------------DIRAGVGCQAPQTVDEALEIANICETFRLPQLQVAAMTAGAVTTTSSSEVNNSQTTNVNPTMNQFIPRAQQMFKAPPIRNPMNNGNGNLNYTNNFRFNNNENNGSVYENCQRFGHQSSDCFKTKTCQRCGKRGHTAVICREFGNGNVRFNGDGNNRGYGRNRINGESRMVTCFTCNQVGHMSRNCPHRMQSNPSEPNQTNANVQINLMQEFERLRNEIEQMKGSNQQQRINMLQENGDVREQIINVNQEQQRAEWQRLQMEEAKEIQARWERRNGTTKRINMMRAVNENEMHTADQCVVNSDGDETSVNED 955

HSP 2 Score: 69.707 bits (169), Expect = 6.207e-12
Identity = 40/53 (75.47%), Postives = 44/53 (83.02%), Query Frame = 2 
Query: 1952 KPS*DDIATKNRIQRILLRKLEEKNITRK--TLTDEQIKQKLEKRWFTTKRWC 2104
            KPS   +ATKNRIQRILL+KLEEK I R+  TLTDEQ+KQKLEKRW T KRWC
Sbjct:  983 KPSKKAMATKNRIQRILLQKLEEK-IRREDETLTDEQVKQKLEKRWATAKRWC 1034
[Close]
BLAST of SMED30017473 vs. Planmine SMEST
Match: SMESG000007496.1 (SMESG000007496.1)

HSP 1 Score: 740.725 bits (1911), Expect = 0.000e+0
Identity = 451/578 (78.03%), Postives = 486/578 (84.08%), Query Frame = 1 
Query:   55 YIFRH--TRCQTGADINKPLSLLEKVEHQAAKR*RSYSRRPAKKPESVETLINTESKNEETGLKXXXXXXXXXXXXXXXXRE*TDQEKNGILREPKVNSRINMTENDTTTPITNSNEMTSQPERVDIGELREKLRGEPFDGSIDKLQTFIKELVVYTTICHWPDIVIRERLPLYLTGSV*DVYLEAKRDPSKLKTWNEIK*FLINTLENAKELNRKMLEFLHRRQKQNE*NVLFACELKRFAREAFGEEKVPVERIVLVFIKGLKHADIRAGMGCQAPQIVDKALEIANIC*KLRLPQQQVAAMTAGAVSTTLSSEFTNSQTTNINPTMNQFIPRSQQMFKVPQTRNPXXXXXXXXXXXXXXXXXXXXXXXPVCEIYQRIGHQRNE*FKAKTCQRCGKRGHTEVTCREFXXXXXXXXXXXXXXXXXXXCVNGEPKIVTCFTCNQVGHMSRNCPHQIQSXXXXXXXXXXXVQINLMQEVERLRNEIQQMKGNNQQQRIIMLKDNGDVREQIINVNQEKQRTEWQRLQMEEAKEIQARWERRNGTMKPINMMRAVNENEIHTAEQCVGNSDGDETSVNED 1782
            +IF H  TR QT AD NK  SLLE VEHQAAKR RSYSRRP+KKPESVET+INTES+NEETGLKLLEDILDN SD+E SDRE TDQE+N IL E KVNS INM E+ TTTPI NSNEMTSQPERVDIGE REK RGEPFDGSIDKLQTFIKE  VYTTICHWPDI IRERLPL LTGS  +VYLEAKRDP+KL+ WNEIK FLINT  NAKELNRK  EFLHR+QKQNE NVLFA ELKR AREAFG++K                 DIRAG+GCQAPQ VD+ALEIANIC   RLPQ QVAAMTAGAV+TT SSE  NSQTTN+NPTMNQFIPR+QQMFK P  RNP NNG GNLNYTNNFRFNNN NNG V E  QR GHQ ++ FK KTCQRCGKRGHT V CREFGN NVR+NG+G+NRGYGRN +NGE ++VTCFTCNQVGHMSRNCPH++QSNP  PN  N NVQINLMQE ERLRNEI+QMKG+NQQQRI ML++NGDVREQIINVNQE+QR EWQRLQMEEAKEIQARWERRNGT K INMMRAVNENE+HTA+QCV NSDGDETSVNED
Sbjct:  395 HIFIHLKTRSQTKADTNKNPSLLETVEHQAAKRSRSYSRRPSKKPESVETIINTESENEETGLKLLEDILDNPSDNEKSDRESTDQEENSILGESKVNSPINMAEDGTTTPIFNSNEMTSQPERVDIGEFREKFRGEPFDGSIDKLQTFIKEFEVYTTICHWPDIAIRERLPLCLTGSDQEVYLEAKRDPTKLRNWNEIKDFLINTFGNAKELNRKTFEFLHRKQKQNESNVLFAFELKRLAREAFGDKK-----------------DIRAGVGCQAPQTVDEALEIANICETFRLPQLQVAAMTAGAVTTTSSSEVNNSQTTNVNPTMNQFIPRAQQMFKAPPIRNPMNNGNGNLNYTNNFRFNNNENNGSVYENCQRFGHQSSDCFKTKTCQRCGKRGHTAVICREFGNGNVRFNGDGNNRGYGRNRINGESRMVTCFTCNQVGHMSRNCPHRMQSNPSEPNQTNANVQINLMQEFERLRNEIEQMKGSNQQQRINMLQENGDVREQIINVNQEQQRAEWQRLQMEEAKEIQARWERRNGTTKRINMMRAVNENEMHTADQCVVNSDGDETSVNED 955

HSP 2 Score: 69.707 bits (169), Expect = 6.517e-12
Identity = 40/53 (75.47%), Postives = 44/53 (83.02%), Query Frame = 2 
Query: 1952 KPS*DDIATKNRIQRILLRKLEEKNITRK--TLTDEQIKQKLEKRWFTTKRWC 2104
            KPS   +ATKNRIQRILL+KLEEK I R+  TLTDEQ+KQKLEKRW T KRWC
Sbjct:  987 KPSKKAMATKNRIQRILLQKLEEK-IRREDETLTDEQVKQKLEKRWATAKRWC 1038
[Close]
BLAST of SMED30017473 vs. Planmine SMEST
Match: SMESG000059107.1 (SMESG000059107.1)

HSP 1 Score: 445.277 bits (1144), Expect = 7.920e-137
Identity = 282/376 (75.00%), Postives = 299/376 (79.52%), Query Frame = 1 
Query:   70 TRCQTGADINKPLSLLEKVEHQAAKR*RSYSRRPAKKPESVETLINTESKNEETGLKXXXXXXXXXXXXXXXXRE*TDQEKNGILREPKVNSRINMTENDTTTPITNSNEMTSQPERVDIGELREKLRGEPFDGSIDKLQTFIKELVVYTTICHWPDIVIRERLPLYLTGSV*DVYLEAKRDPSKLKTWNEIK*FLINTLENAKELNRKMLEFLHRRQKQNE*NVLFACELKRFAREAFGEEKVPVERIVLVFIKGLKHADIRAGMGCQAPQIVDKALEIANIC*KLRLPQQQVAAMTAGAVSTTLSSEFTNSQTTNINPTMNQFIPRSQQMFKVPQTRNPXXXXXXXXXXXXXXXXXXXXXXXPVCEIYQRIGHQ 1197
            TR QT ADINK  SLLE VEHQAAKR RSYSRRP+KKPESVET+INTES+NEETGLKLLEDIL++ SD+ENSDRE TDQE+N IL E KVNS INM E+ TTT I NSNE TSQPERVDIGE REK RGEPF GSIDKLQTFIKE  VYTTIC WPDI IRERL LYLT S  DV+LEAKRDPSKLK WNEIK FLINT  NAKELNRK+                          EAFG+EKVP ERIV VFIKGLKH DIRAG+GCQAPQ VDKALEIANIC   RLPQ QVAAMTAGA++TT +SE  NSQTTN+NPTMNQFIPR+QQMFK P  RNP NNG GNLNYTNNFRFNNNGNNGPVCE  QR GHQ
Sbjct: 1557 TRSQTRADINKNPSLLETVEHQAAKRSRSYSRRPSKKPESVETIINTESENEETGLKLLEDILNDPSDNENSDRESTDQEENSILGESKVNSPINMAEDGTTTSIFNSNETTSQPERVDIGEFREKFRGEPFAGSIDKLQTFIKEFEVYTTICQWPDIAIRERLSLYLTESAQDVHLEAKRDPSKLKNWNEIKDFLINTFGNAKELNRKI--------------------------EAFGDEKVPEERIVSVFIKGLKHEDIRAGVGCQAPQTVDKALEIANICETFRLPQLQVAAMTAGAITTTSNSEVNNSQTTNVNPTMNQFIPRAQQMFKAPPIRNPMNNGNGNLNYTNNFRFNNNGNNGPVCENCQRFGHQ 1906

HSP 2 Score: 202.601 bits (514), Expect = 1.506e-66
Identity = 138/158 (87.34%), Postives = 146/158 (92.41%), Query Frame = 1 
Query: 1480 VERLRNEIQQMKGNNQQQRIIMLKDNGDVREQIINVNQEKQRTEWQRLQMEEAKEIQARWERRNGTMKPINMMRAVNENEIHTAEQCVGNSDGDETSVNEDVLPENIIKCETNKLTSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNDETKTIKK 1953
            VERLRNEI+QMKG+NQQQRI ML++N DVREQ INVNQ +QR EWQRLQMEEAKEIQARWERRNGT K INMM AVNE+EIHTAEQCV NSDGDE SVNEDVLPEN IKCET+KLTSEEKIKRIQEFIKEKKEKRREIKEIQMKIEE+QNDETKTI K
Sbjct: 1909 VERLRNEIEQMKGSNQQQRINMLQENEDVREQTINVNQGQQRAEWQRLQMEEAKEIQARWERRNGTTKRINMMEAVNEDEIHTAEQCVVNSDGDEMSVNEDVLPENKIKCETSKLTSEEKIKRIQEFIKEKKEKRREIKEIQMKIEEIQNDETKTITK 2066

HSP 3 Score: 71.2478 bits (173), Expect = 1.506e-66
Identity = 40/53 (75.47%), Postives = 45/53 (84.91%), Query Frame = 2 
Query: 1952 KPS*DDIATKNRIQRILLRKLEEKNITRK--TLTDEQIKQKLEKRWFTTKRWC 2104
            KPS + +ATKNRIQRILL+KLEEK I R+  TLTDEQ+KQKLE RW TTKRWC
Sbjct: 2066 KPSKEAMATKNRIQRILLQKLEEK-IRREDETLTDEQVKQKLETRWATTKRWC 2117
[Close]
BLAST of SMED30017473 vs. Planmine SMEST
Match: SMESG000074160.1 (SMESG000074160.1)

HSP 1 Score: 360.533 bits (924), Expect = 1.317e-107
Identity = 221/481 (45.95%), Postives = 285/481 (59.25%), Query Frame = 1 
Query:  331 PKVNSRINMTENDTTTPITNSNEMTSQPERVDIGELREKLRGEPFDGSIDKLQTFIKELVVYTTICHWPDIVIRERLPLYLTGSV*DVYLEAKRDPSKLKTWNEIK*FLINTLENAKELNRKMLEFLHRRQKQNE*NVLFACELKRFAREAFGEEKVPVERIVLVFIKGLKHADIRAGMGCQAPQIVDKALEIANIC*KLRLPQQQVAAMTAGAVSTTLSSEFTNSQTTNINPTMNQFIPRSQQMFKVPQTRNPXXXXXXXXXXXXXXXXXXXXXXXPVCEIYQRIGHQRNE*FKAKTCQRCGKRGHTEVTCREFXXXXXXXXXXXXXXXXXXXCVNGEPKIVTCFTCNQVGHMSRNCPHQIQSXXXXXXXXXXXVQINLMQEVERLRNEI-----------QQMKGNNQQQRIIMLKDNGDVREQIINVNQEKQRTEWQRLQMEEAKEIQARWERRNGTMKPINMMRAVNENEIHTAEQC 1740
            P + + IN +  D  T + N+N          +GE REK RGEPFDGS++KL TFIKE  VY  ICHW D  ++ERLPLYL GS  DV++E  RD +KL TW EIK FLI      K+ NRK+L+FLHR+Q+++E N ++ACELK+  +EAFGE  +P +++V VFI+G+K  DIRA +GC AP+ +DKA+ IAN C        QVA +   A   T S+   N+Q  N NP MNQFIPR+QQMFK P TRNP + G   ++Y NNFR N N N    C   QRIGH+    F+   CQ CGKRGHTE  CR                         +P    C+ C Q GH++R C  + + N    N+   N QIN+MQE++ LR  +           QQM  N QQQRI +++ N +++E      QE+Q+ EWQRLQ EEA+EIQ RWE R  T K INMMR    +E HTAEQC
Sbjct:  164 PNMTTSINSSIPDANTMLNNNN--------ATVGEFREKFRGEPFDGSLNKLDTFIKEFEVYKNICHWTDQKVKERLPLYLKGSAQDVFVEEARDATKLTTWIEIKEFLIKIFGIEKKGNRKILDFLHRKQRRDESNAVYACELKKLCKEAFGEADLPEDKMVDVFIRGIKREDIRANLGCLAPETLDKAVAIANRCEAHLGLGYQVAVL---ATEPTTSTVNANNQNNNTNPAMNQFIPRAQQMFKPPVTRNPEDKGNNQVSYNNNFRNNPNANEK--CSTCQRIGHRSENCFRNYNCQICGKRGHTEKICR-------------------------QPNNKACYNCGQQGHLARQCNIRREPNQSRLNNSMGNPQINVMQEIDMLRETLQRMPMELKEMMQQMPTNIQQQRINVMRSNEEIQELRTREYQEQQQAEWQRLQTEEAQEIQQRWENRKQTPKRINMMRMEIPDETHTAEQC 606
The following BLAST results are available for this feature:
BLAST of SMED30017473 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017473 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000007496.15.568e-1278.44SMESG000007496.1[more]
SMESG000007496.16.207e-1278.03SMESG000007496.1[more]
SMESG000007496.16.517e-1278.03SMESG000007496.1[more]
SMESG000059107.17.920e-13775.00SMESG000059107.1[more]
SMESG000074160.11.317e-10745.95SMESG000074160.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017473 ID=SMED30017473|Name=SMED30017473|organism=Schmidtea mediterranea sexual|type=transcript|length=2104bp
CCCGGTGGAAATAAAAATAAAGATATAAGTCAAAGAACTTAATCCAAAAT
TGATTACATATTCAGACATACCAGATGTCAAACAGGAGCAGACATCAATA
AACCCCTCAGTTTATTAGAAAAAGTAGAACACCAAGCCGCAAAAAGATAG
AGAAGTTATTCAAGAAGACCGGCAAAGAAACCAGAATCAGTCGAAACACT
AATAAACACCGAATCAAAGAACGAAGAAACAGGACTTAAATTACTTGAGG
ATATTCTAGACAATTCATCAGACAGCGAGAACAGCGACAGAGAATAGACA
GACCAAGAGAAAAACGGTATTTTAAGAGAACCAAAAGTCAATAGTCGAAT
CAACATGACAGAGAACGATACGACGACACCCATTACCAACAGCAATGAAA
TGACATCACAACCCGAAAGAGTGGATATCGGGGAGCTTAGAGAAAAATTG
CGAGGTGAACCATTTGACGGAAGTATCGATAAATTACAAACGTTTATCAA
AGAATTGGTGGTGTATACGACAATATGTCATTGGCCAGATATAGTAATTA
GAGAGAGACTGCCACTGTATTTAACAGGGAGCGTCTAAGATGTATATTTA
GAAGCCAAACGAGACCCAAGCAAACTTAAAACCTGGAACGAGATTAAATA
GTTCCTAATTAACACATTGGAAAACGCGAAAGAGCTCAACCGAAAAATGT
TAGAATTCCTACACCGAAGGCAAAAACAAAACGAATAAAATGTACTATTT
GCGTGTGAACTAAAACGATTTGCAAGAGAAGCTTTTGGCGAAGAGAAAGT
ACCGGTGGAAAGAATAGTCTTAGTATTCATAAAAGGACTAAAACATGCGG
ATATCAGAGCAGGCATGGGATGCCAAGCACCGCAAATAGTAGATAAAGCA
CTTGAAATCGCCAATATATGCTAAAAATTACGACTACCACAACAACAAGT
AGCAGCAATGACAGCAGGAGCGGTTTCAACAACATTAAGCAGTGAATTTA
CCAACAGTCAGACAACTAATATCAATCCAACGATGAATCAATTCATTCCA
AGGTCACAACAGATGTTCAAGGTTCCACAGACAAGAAATCCAACTAATAA
TGGTATTGGTAATTTGAATTACACTAATAATTTCAGATTCAATAACAACG
GAAATAACGGACCAGTATGCGAGATTTATCAGAGAATCGGTCATCAAAGA
AACGAGTGATTCAAAGCAAAAACATGTCAGAGATGCGGTAAAAGAGGCCA
TACGGAAGTAACCTGCAGAGAATTCGGGAACAGAAATGTCAGATACAACG
GAAACGGCAGCAACAGAGGATACGGCAGAAATTGTGTTAATGGCGAACCG
AAAATAGTAACTTGCTTCACATGTAATCAAGTGGGACACATGTCAAGGAA
TTGCCCACATCAGATACAATCAAATCCACCTGGACCAAACCATGGAAATC
CAAACGTACAAATCAATCTGATGCAAGAAGTTGAACGACTGCGAAACGAA
ATACAACAAATGAAAGGCAATAATCAACAACAACGCATAATCATGCTGAA
GGACAACGGAGATGTCAGAGAGCAAATAATAAATGTCAACCAAGAAAAAC
AGAGGACGGAATGGCAAAGACTACAAATGGAAGAAGCAAAAGAGATCCAA
GCAAGATGGGAACGCCGAAACGGAACTATGAAACCCATAAATATGATGAG
AGCAGTAAATGAAAACGAAATTCACACTGCGGAGCAGTGTGTTGGGAACA
GCGACGGTGACGAAACGAGTGTTAACGAGGATGTATTGCCAGAAAATATA
ATAAAATGTGAAACGAATAAATTGACTAGTGAAGAGAAAATAAAACGAAT
CCAAGAATTTATCAAAGAGAAAAAAGAGAAAAGACGAGAAATTAAAGAAA
TCCAAATGAAAATTGAAGAAATGCAAAATGACGAGACAAAAACAATAAAA
AAAACCTTCATAGGATGATATAGCTACAAAAAATCGAATACAAAGGATCC
TACTACGGAAATTAGAAGAAAAAAATATAACGAGAAAAACGTTGACGGAT
GAACAAATCAAACAAAAACTAGAAAAGAGATGGTTCACAACTAAAAGATG
GTGC
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protein sequence of SMED30017473-orf-1

>SMED30017473-orf-1 ID=SMED30017473-orf-1|Name=SMED30017473-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=193bp
MSRNCPHQIQSNPPGPNHGNPNVQINLMQEVERLRNEIQQMKGNNQQQRI
IMLKDNGDVREQIINVNQEKQRTEWQRLQMEEAKEIQARWERRNGTMKPI
NMMRAVNENEIHTAEQCVGNSDGDETSVNEDVLPENIIKCETNKLTSEEK
IKRIQEFIKEKKEKRREIKEIQMKIEEMQNDETKTIKKTFIG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR001878Znf_CCHC
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0008270zinc ion binding