SMED30017463

Overview
NameSMED30017463
Smed IDSMED30017463
Length (bp)4236
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30017463 (SMED30017463) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017463 (SMED30017463) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017463 (SMED30017463) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017463 (SMED30017463) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017463

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30017463h1SMcG0020944 SmedASXL_013871SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ventral epidermisSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
whole organismSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
dorsal region of the epidermisSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
non-ciliated epidermisSMED30017463h1SMcG0020944 dd_Smed_v4_4940_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30017463 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000265:99199..121888 +20502
Homology
[Close]
BLAST of SMED30017463 vs. Planmine SMEST
Match: SMESG000016833.1 (SMESG000016833.1)

HSP 1 Score: 2615.88 bits (6779), Expect = 0.000e+0
Identity = 1352/1363 (99.19%), Postives = 1355/1363 (99.41%), Query Frame = 3 
Query:   42 MISYDNYENQNMFQDQYEIQSDSNSKDELTLITGNNLTERAQSLIDDDVGSNTNLDFIPVRDRCSKNKQSNSADEIEIIDVPIKIQSYEDKSKIYKCEDSNEGWKNMNYKRNNANRDVDEYDLEGNRYGFADSNRYCDELLHTVRHIAQPSVTLTKHENRRLDEPCQHRNYQPVFNQMNEPSSPSCYSPVTTDIGRQITQLARRIESLGNISRICQDDIENYSVIPKCDTSYCSPDNLIAMPHQESHISDNYSVKAARKLDEIGRLQKKQEAMQNRLVDLAKKLNDSTRDLTRIPSKPILNEEPVTESDYFHGXXXXXXXXXXXXXXXXXXXXITKPHQSSFLKNILNDANEGKTTELPLLSSSYCLHNDLSMGTAESGISESTSKYLSDELANIRQEFRTQVEEIRKLLSERYQQSIEQPKKPEIVQQPYELLSDQIAKSVQKLSRDNSPSLKXXXXXXXXXXSECPLDKYLIKSNKIEELPSLHSEIVPSLRPGILPSVARAIKSKQRMLSDPVEAEKFLIXXXXXXXXXXXXXXXXXXTKEKEFLFSILERLQGDENNVDLARIQAMVDDTIASINPVVKFEVKQEYLLWIERRKEQREQQLIAEEEEKQFINKLKSTKQTKLQRPVHHHPLPSASAINGKPWICKKYLSVRSKVDSGVKKQPEIPISRVARPQSPSRHTNLPSASTHATWSNRRRYRSPYLHFINDVKLAKKSINPLGDGTRKIPIITHTETKSVKRRSRSSPQRQNDDLILMSVKQYSLDYKEDEEQIVPIEKPQLVAKSTSPRTISVPESTSTVSRNSYDNKHDTNQSNKNWATQTQNQSHYXXXXXXXXXXXXKGTGFQLKSKPSLKPREERVGTYVPMDPTETTKLVSHPVHVMLSKPQPVAEPDINGLRLLIERALLDQIQIPSPGASYEESETVTRVGNVATPLPTDGGDEYSSHFSPEHSTKEHYDRTLSYVDNIKTPEISQIISEHSFRPTRVSTPSNSLNTXXXXXXXXXXXXQMVSEAIQTICENAVQTEEDKEIQTIPVQSGTQTPKSPRKPPSDTSAGSITSVFDESQSDTQAHSFSDGMWLYDRSEGEMAPYQIETVSRWKLGNCPENPLWYNGTDDIINVDRQSIASFGEFTKPTLPGLIPTLDPVLQLKTLPFKPSYETPDINKDDDFMYMSHRQQSFPTNFKSLGVSRDSKSKFPTPQNDQMSSGNFNPSEGEINDKEDGNDYVSEEEDKEPDNFNYRSSEDFRPESPFLMNVKQSKGSGRSDTIDPVRDSQSDRMHIDVNKYLRASKQSHSDMSDTAHLMSTNQIVENAFRGGDSADFEGYSDYGNDRHIGDNPNANKQTVKIPTLGTVEQSNPSISDDNLE 4130
            MISYDNYENQNMFQDQYEIQSDSNSKDELTLITGNNLTERAQSLIDDDVGSNTNLDFIPVRDRCSKNKQSNSADEIEIIDVPIKIQSYEDKSKIYKCEDSNE WKNMNYKRNNANRDVDEYDLEGNRYGFADSNRYCDELLHTVRHIAQPSVTLTKHENRRLDEPCQHRNYQPVFNQMNEPSSPSCYSPVTTDIGRQITQLARRIESLGNISRICQDDIENYSVIPKCDTSYCSPDNLIAMPHQESHISDNYSVKAARKLDEIGRLQKKQEAMQNRLVDLAKKLNDSTRDLTRIPSKPILNEEPVTESDYFHGKVAKPSKKSSKKLKQKNRKSITKPHQSSFLKNILNDANEGKTTELPLLSSSYCLHNDLSMGTAESGISESTSKYLSDELANIRQEFRTQVEEIRKLLSERYQQSIEQPKKPEIVQQPYE LSDQIAKSVQKLSRDNSPSLKNITNINNNYNSECPLDKYLIKSNK EELPSLHSEIVPSLRPGILPSVARAIKSKQRMLSDPVEAEKFLIELANRRANLENNLRCLERTKEKEFLFSILERLQGDENNVDLARIQAMVDDTIASINPVVKFEVKQEYLLWIERRKEQREQQLIAEEEEKQFINKLKSTKQTKLQRPVHHHPLPSASAINGKPWICKKYLSVRSKVDSGVKKQPEIPISRVARPQSPSRHTNLPSASTHATWSNRRRYRSPYLHFINDVKLAKKSINPLGDGTRKIPIITHTETKSVKRRSR+SPQRQNDDLILMSVKQYSLDYKEDEEQIVPIEKPQLVAKSTSPRTISVPESTS VSRNSYDNKHDTNQSNKNWATQTQNQSHYSEPAPPSPSLSEKGTGFQLKSKPSLKPREERVGTY PMDPTETTKLVSHPVHVMLSKPQPVAEPDI+GLRLLIERALLDQIQIPSPGASYEESETVTRVGNVATPLPTDGGDEYSSHFSPEHSTKEHYDRTLSYVDNIKTPEISQIISEHSFRPTRVSTPSNSLNTSHKSSPIKSPKHQMVSEAIQTICENA QTEEDKEIQTIPVQSGTQTPKSPRKPPSDTSAGSITSVFDESQSDTQAHSFSDGMWLYDRSEGEMAPYQIETVSRWKLGNCPENPLWYNGTDDIINVDRQSIASFGEFTKPTLPGLIPTLDPVLQLKTLPFKPSYETPDINKDDDFMYMSHRQQSFPTNFKSLGVSRDSKSKFPT QNDQMSSGNFNPSEGEIND+EDGNDYVSEEEDKEPDNFNYRSSEDFRPESPFLMNVKQSKGSGRSDTIDPVRDSQSDRMHIDVNKYLRASKQSHSDMSDTAHLMSTNQIVENAFRGGDSADFEGYSDYGNDRHIGDNPNA KQTVKIPTLGTVEQSNPSISDDNLE
Sbjct:    1 MISYDNYENQNMFQDQYEIQSDSNSKDELTLITGNNLTERAQSLIDDDVGSNTNLDFIPVRDRCSKNKQSNSADEIEIIDVPIKIQSYEDKSKIYKCEDSNEFWKNMNYKRNNANRDVDEYDLEGNRYGFADSNRYCDELLHTVRHIAQPSVTLTKHENRRLDEPCQHRNYQPVFNQMNEPSSPSCYSPVTTDIGRQITQLARRIESLGNISRICQDDIENYSVIPKCDTSYCSPDNLIAMPHQESHISDNYSVKAARKLDEIGRLQKKQEAMQNRLVDLAKKLNDSTRDLTRIPSKPILNEEPVTESDYFHGKVAKPSKKSSKKLKQKNRKSITKPHQSSFLKNILNDANEGKTTELPLLSSSYCLHNDLSMGTAESGISESTSKYLSDELANIRQEFRTQVEEIRKLLSERYQQSIEQPKKPEIVQQPYESLSDQIAKSVQKLSRDNSPSLKNITNINNNYNSECPLDKYLIKSNKTEELPSLHSEIVPSLRPGILPSVARAIKSKQRMLSDPVEAEKFLIELANRRANLENNLRCLERTKEKEFLFSILERLQGDENNVDLARIQAMVDDTIASINPVVKFEVKQEYLLWIERRKEQREQQLIAEEEEKQFINKLKSTKQTKLQRPVHHHPLPSASAINGKPWICKKYLSVRSKVDSGVKKQPEIPISRVARPQSPSRHTNLPSASTHATWSNRRRYRSPYLHFINDVKLAKKSINPLGDGTRKIPIITHTETKSVKRRSRASPQRQNDDLILMSVKQYSLDYKEDEEQIVPIEKPQLVAKSTSPRTISVPESTSPVSRNSYDNKHDTNQSNKNWATQTQNQSHYSEPAPPSPSLSEKGTGFQLKSKPSLKPREERVGTYAPMDPTETTKLVSHPVHVMLSKPQPVAEPDIHGLRLLIERALLDQIQIPSPGASYEESETVTRVGNVATPLPTDGGDEYSSHFSPEHSTKEHYDRTLSYVDNIKTPEISQIISEHSFRPTRVSTPSNSLNTSHKSSPIKSPKHQMVSEAIQTICENAAQTEEDKEIQTIPVQSGTQTPKSPRKPPSDTSAGSITSVFDESQSDTQAHSFSDGMWLYDRSEGEMAPYQIETVSRWKLGNCPENPLWYNGTDDIINVDRQSIASFGEFTKPTLPGLIPTLDPVLQLKTLPFKPSYETPDINKDDDFMYMSHRQQSFPTNFKSLGVSRDSKSKFPTIQNDQMSSGNFNPSEGEINDEEDGNDYVSEEEDKEPDNFNYRSSEDFRPESPFLMNVKQSKGSGRSDTIDPVRDSQSDRMHIDVNKYLRASKQSHSDMSDTAHLMSTNQIVENAFRGGDSADFEGYSDYGNDRHIGDNPNATKQTVKIPTLGTVEQSNPSISDDNLE 1363
[Close]
BLAST of SMED30017463 vs. Planmine SMEST
Match: SMESG000016833.1 (SMESG000016833.1)

HSP 1 Score: 2545.77 bits (6597), Expect = 0.000e+0
Identity = 1326/1363 (97.29%), Postives = 1329/1363 (97.51%), Query Frame = 3 
Query:   42 MISYDNYENQNMFQDQYEIQSDSNSKDELTLITGNNLTERAQSLIDDDVGSNTNLDFIPVRDRCSKNKQSNSADEIEIIDVPIKIQSYEDKSKIYKCEDSNEGWKNMNYKRNNANRDVDEYDLEGNRYGFADSNRYCDELLHTVRHIAQPSVTLTKHENRRLDEPCQHRNYQPVFNQMNEPSSPSCYSPVTTDIGRQITQLARRIESLGNISRICQDDIENYSVIPKCDTSYCSPDNLIAMPHQESHISDNYSVKAARKLDEIGRLQKKQEAMQNRLVDLAKKLNDSTRDLTRIPSKPILNEEPVTESDYFHGXXXXXXXXXXXXXXXXXXXXITKPHQSSFLKNILNDANEGKTTELPLLSSSYCLHNDLSMGTAESGISESTSKYLSDELANIRQEFRTQVEEIRKLLSERYQQSIEQPKKPEIVQQPYELLSDQIAKSVQKLSRDNSPSLKXXXXXXXXXXSECPLDKYLIKSNKIEELPSLHSEIVPSLRPGILPSVARAIKSKQRMLSDPVEAEKFLIXXXXXXXXXXXXXXXXXXTKEKEFLFSILERLQGDENNVDLARIQAMVDDTIASINPVVKFEVKQEYLLWIERRKEQREQQLIAEEEEKQFINKLKSTKQTKLQRPVHHHPLPSASAINGKPWICKKYLSVRSKVDSGVKKQPEIPISRVARPQSPSRHTNLPSASTHATWSNRRRYRSPYLHFINDVKLAKKSINPLGDGTRKIPIITHTETKSVKRRSRSSPQRQNDDLILMSVKQYSLDYKEDEEQIVPIEKPQLVAKSTSPRTISVPESTSTVSRNSYDNKHDTNQSNKNWATQTQNQSHYXXXXXXXXXXXXKGTGFQLKSKPSLKPREERVGTYVPMDPTETTKLVSHPVHVMLSKPQPVAEPDINGLRLLIERALLDQIQIPSPGASYEESETVTRVGNVATPLPTDGGDEYSSHFSPEHSTKEHYDRTLSYVDNIKTPEISQIISEHSFRPTRVSTPSNSLNTXXXXXXXXXXXXQMVSEAIQTICENAVQTEEDKEIQTIPVQSGTQTPKSPRKPPSDTSAGSITSVFDESQSDTQAHSFSDGMWLYDRSEGEMAPYQIETVSRWKLGNCPENPLWYNGTDDIINVDRQSIASFGEFTKPTLPGLIPTLDPVLQLKTLPFKPSYETPDINKDDDFMYMSHRQQSFPTNFKSLGVSRDSKSKFPTPQNDQMSSGNFNPSEGEINDKEDGNDYVSEEEDKEPDNFNYRSSEDFRPESPFLMNVKQSKGSGRSDTIDPVRDSQSDRMHIDVNKYLRASKQSHSDMSDTAHLMSTNQIVENAFRGGDSADFEGYSDYGNDRHIGDNPNANKQTVKIPTLGTVEQSNPSISDDNLE 4130
            MISYDNYENQNMFQDQYEIQSDSNSKDELTLITGNNLTERAQSLIDDDVGSNTNLDFIPVRDRCSKNKQSNSADEIEIIDVPIKIQSYEDKSKIYKCEDSNE WKNMNYKRNNANRDVDEYDLEGNRYGFADSNRYCDELLHTVRHIAQPSVTLTKHENRRLDEPCQHRNYQPVFNQMNEPSSPSCYSPVTTDIGRQITQLARRIESLGNISRICQDDIENYSVIPKCDTSYCSPDNLIAMPHQESHISDNYSVKAARKLDEIGRLQKKQEAMQNRLVDLAKKLNDSTRDLTRIPSKPILNEEPVTESDYFHGKVAKPSKKSSKKLKQKNRKSITKPHQSSFLKNILNDANEGKTTELPLLSSSYCLHNDLSMGTAESGISESTSKYLSDELANIRQEFRTQVEEIRKLLSERYQQSIEQPKKPEIVQQPYE LSDQIAKSVQKLSRDNSPSLKNITNINNNYNSECPLDKYLIKSNK EELPSLHSEIVPSLRPGILPSVARAIKSKQRMLSDPVEAEKFLIELANRRANLENNLRCLERTKEKEFLFSILERLQGDENNVDLARIQAMVDDTIASINPVVKFEVKQEYLLWIERRKEQREQQLIAEEEEKQFINKLKSTKQTKLQRPVHHHPLPSASAINGKPWICKKYLSVRSKVDSGVKKQPEIPISRVARPQSPSRHTNLPSASTHATWSNRRRYRSPYLHFINDVKLAKKSINPLGDGTRKIPIITHTETKSVKRRSR+SPQRQNDDLILMSVKQYSLDYKEDEEQIVPIEKPQLVAKSTSPRTISVPES                           NQSHYSEPAPPSPSLSEKGTGFQLKSKPSLKPREERVGTY PMDPTETTKLVSHPVHVMLSKPQPVAEPDI+GLRLLIERALLDQIQIPSPGASYEESETVTRVGNVATPLPTDGGDEYSSHFSPEHSTKEHYDRTLSYVDNIKTPEISQIISEHSFRPTRVSTPSNSLNTSHKSSPIKSPKHQMVSEAIQTICENA QTEEDKEIQTIPVQSGTQTPKSPRKPPSDTSAGSITSVFDESQSDTQAHSFSDGMWLYDRSEGEMAPYQIETVSRWKLGNCPENPLWYNGTDDIINVDRQSIASFGEFTKPTLPGLIPTLDPVLQLKTLPFKPSYETPDINKDDDFMYMSHRQQSFPTNFKSLGVSRDSKSKFPT QNDQMSSGNFNPSEGEIND+EDGNDYVSEEEDKEPDNFNYRSSEDFRPESPFLMNVKQSKGSGRSDTIDPVRDSQSDRMHIDVNKYLRASKQSHSDMSDTAHLMSTNQIVENAFRGGDSADFEGYSDYGNDRHIGDNPNA KQTVKIPTLGTVEQSNPSISDDNLE
Sbjct:    1 MISYDNYENQNMFQDQYEIQSDSNSKDELTLITGNNLTERAQSLIDDDVGSNTNLDFIPVRDRCSKNKQSNSADEIEIIDVPIKIQSYEDKSKIYKCEDSNEFWKNMNYKRNNANRDVDEYDLEGNRYGFADSNRYCDELLHTVRHIAQPSVTLTKHENRRLDEPCQHRNYQPVFNQMNEPSSPSCYSPVTTDIGRQITQLARRIESLGNISRICQDDIENYSVIPKCDTSYCSPDNLIAMPHQESHISDNYSVKAARKLDEIGRLQKKQEAMQNRLVDLAKKLNDSTRDLTRIPSKPILNEEPVTESDYFHGKVAKPSKKSSKKLKQKNRKSITKPHQSSFLKNILNDANEGKTTELPLLSSSYCLHNDLSMGTAESGISESTSKYLSDELANIRQEFRTQVEEIRKLLSERYQQSIEQPKKPEIVQQPYESLSDQIAKSVQKLSRDNSPSLKNITNINNNYNSECPLDKYLIKSNKTEELPSLHSEIVPSLRPGILPSVARAIKSKQRMLSDPVEAEKFLIELANRRANLENNLRCLERTKEKEFLFSILERLQGDENNVDLARIQAMVDDTIASINPVVKFEVKQEYLLWIERRKEQREQQLIAEEEEKQFINKLKSTKQTKLQRPVHHHPLPSASAINGKPWICKKYLSVRSKVDSGVKKQPEIPISRVARPQSPSRHTNLPSASTHATWSNRRRYRSPYLHFINDVKLAKKSINPLGDGTRKIPIITHTETKSVKRRSRASPQRQNDDLILMSVKQYSLDYKEDEEQIVPIEKPQLVAKSTSPRTISVPES---------------------------NQSHYSEPAPPSPSLSEKGTGFQLKSKPSLKPREERVGTYAPMDPTETTKLVSHPVHVMLSKPQPVAEPDIHGLRLLIERALLDQIQIPSPGASYEESETVTRVGNVATPLPTDGGDEYSSHFSPEHSTKEHYDRTLSYVDNIKTPEISQIISEHSFRPTRVSTPSNSLNTSHKSSPIKSPKHQMVSEAIQTICENAAQTEEDKEIQTIPVQSGTQTPKSPRKPPSDTSAGSITSVFDESQSDTQAHSFSDGMWLYDRSEGEMAPYQIETVSRWKLGNCPENPLWYNGTDDIINVDRQSIASFGEFTKPTLPGLIPTLDPVLQLKTLPFKPSYETPDINKDDDFMYMSHRQQSFPTNFKSLGVSRDSKSKFPTIQNDQMSSGNFNPSEGEINDEEDGNDYVSEEEDKEPDNFNYRSSEDFRPESPFLMNVKQSKGSGRSDTIDPVRDSQSDRMHIDVNKYLRASKQSHSDMSDTAHLMSTNQIVENAFRGGDSADFEGYSDYGNDRHIGDNPNATKQTVKIPTLGTVEQSNPSISDDNLE 1336
The following BLAST results are available for this feature:
BLAST of SMED30017463 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017463 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000016833.10.000e+099.19SMESG000016833.1[more]
SMESG000016833.10.000e+097.29SMESG000016833.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017463 ID=SMED30017463|Name=SMED30017463|organism=Schmidtea mediterranea sexual|type=transcript|length=4236bp
TACTAACTAAAATATCAAATATACAATTTTAAGTATATCCAATGATTAGT
TATGATAATTATGAAAATCAAAATATGTTTCAAGACCAGTATGAAATACA
GTCTGATTCAAACTCGAAAGATGAATTAACATTAATTACTGGAAATAATT
TAACTGAACGTGCTCAAAGTTTAATTGATGATGATGTAGGATCAAACACT
AATTTAGATTTTATACCGGTAAGAGATAGATGTTCAAAAAATAAACAATC
TAACTCAGCAGATGAAATTGAAATAATTGATGTTCCCATAAAAATCCAAT
CATATGAAGATAAATCAAAAATATACAAATGTGAAGATTCAAACGAGGGT
TGGAAAAATATGAATTATAAACGGAATAATGCCAATCGTGATGTCGATGA
ATATGATTTGGAAGGAAATCGTTACGGATTTGCTGATAGCAATAGATACT
GTGATGAGCTTTTACATACTGTAAGACATATTGCTCAACCTTCCGTTACT
CTGACTAAACACGAAAACCGTCGTTTGGATGAACCTTGTCAACATCGAAA
TTATCAGCCAGTATTTAATCAAATGAATGAGCCGTCTTCACCATCATGTT
ATTCTCCAGTGACTACCGATATTGGTAGACAAATCACTCAACTTGCTAGA
CGAATAGAAAGTCTGGGAAATATTAGTCGTATTTGTCAAGACGATATTGA
AAATTATTCTGTCATTCCTAAATGTGATACTTCTTACTGCTCGCCAGATA
ATCTCATTGCTATGCCTCACCAAGAATCGCATATCTCGGATAACTATTCC
GTCAAAGCCGCTCGAAAACTCGATGAAATTGGACGACTCCAAAAGAAACA
GGAAGCGATGCAAAATCGACTTGTTGATTTGGCAAAAAAACTAAACGACT
CGACTCGTGATTTAACTCGCATTCCAAGCAAACCGATACTCAATGAAGAA
CCCGTCACTGAGAGTGATTATTTTCACGGGAAAGTAGCAAAACCTTCAAA
GAAGTCTTCGAAAAAACTCAAACAGAAAAATCGAAAGTCCATTACAAAAC
CTCATCAGTCTTCGTTCTTAAAAAATATCCTCAATGACGCCAATGAGGGC
AAAACAACTGAGTTGCCATTGTTGAGTAGTAGCTATTGTCTGCATAACGA
TCTGTCAATGGGGACTGCCGAGAGCGGGATATCTGAATCCACTAGTAAAT
ATTTGAGTGATGAATTGGCAAATATTCGTCAGGAATTCCGAACCCAAGTC
GAGGAAATCCGGAAATTATTGAGTGAAAGATATCAGCAATCGATTGAGCA
ACCTAAAAAACCGGAAATCGTTCAACAGCCTTATGAATTACTGTCCGATC
AAATCGCAAAGTCCGTTCAGAAGCTCAGTCGGGACAACTCTCCCAGCCTT
AAAAACATAACCAATATAAACAACAACTACAATTCCGAATGCCCATTGGA
TAAATATCTAATTAAAAGCAACAAAATTGAAGAGTTGCCATCTTTACATT
CGGAAATCGTTCCGAGTCTCCGTCCGGGAATTCTACCAAGCGTTGCCAGA
GCCATTAAAAGCAAACAGCGAATGCTCAGTGATCCGGTTGAGGCGGAAAA
GTTCCTCATTGAATTGGCCAATCGTCGCGCTAATTTAGAAAACAATCTTA
GATGTCTTGAGCGAACGAAAGAAAAAGAATTTCTATTTTCCATTCTAGAA
AGACTACAAGGAGACGAAAACAATGTGGATTTGGCACGCATCCAAGCGAT
GGTAGATGATACAATAGCCTCCATCAACCCTGTTGTTAAATTTGAAGTAA
AACAAGAATATTTGCTATGGATTGAAAGGCGAAAAGAGCAAAGAGAACAA
CAGTTGATAGCGGAAGAAGAGGAGAAACAGTTCATTAATAAATTAAAGTC
CACAAAACAAACAAAACTGCAGAGACCAGTCCATCATCACCCACTTCCAT
CGGCTTCAGCAATTAATGGGAAACCGTGGATTTGTAAAAAGTATTTATCC
GTTCGCTCGAAAGTCGATTCTGGTGTGAAAAAGCAACCGGAAATTCCAAT
TTCAAGAGTTGCGCGTCCTCAATCGCCGTCAAGACATACAAATCTACCGT
CAGCTAGCACTCACGCGACATGGTCCAATCGTCGTCGATATCGAAGTCCT
TATTTGCATTTTATCAATGATGTTAAATTGGCAAAGAAATCGATCAACCC
TTTGGGAGATGGAACGCGCAAGATTCCAATAATCACTCACACTGAGACTA
AATCAGTAAAACGCAGGTCCAGATCAAGTCCACAACGACAGAATGATGAC
CTCATATTGATGAGCGTGAAGCAATACTCACTCGACTATAAAGAAGATGA
GGAACAAATAGTTCCAATAGAAAAACCACAATTGGTTGCCAAATCAACCT
CTCCTCGTACTATTTCAGTTCCAGAATCAACCAGCACGGTATCCAGGAAT
TCATATGACAATAAACATGACACAAATCAGTCTAATAAAAATTGGGCAAC
TCAAACTCAAAATCAGTCTCATTACTCTGAGCCAGCTCCACCCAGTCCTT
CATTATCAGAAAAAGGTACAGGATTTCAACTGAAGAGTAAACCGAGTTTA
AAGCCGCGAGAAGAAAGAGTGGGAACTTATGTGCCGATGGATCCCACTGA
AACGACGAAACTGGTGTCACATCCTGTCCATGTAATGCTATCTAAACCAC
AGCCAGTTGCCGAACCTGATATCAACGGTCTTCGACTGTTAATTGAACGA
GCACTTCTTGATCAAATTCAAATTCCGTCTCCAGGTGCATCATATGAGGA
GTCTGAAACAGTGACAAGGGTGGGAAATGTTGCAACTCCATTGCCGACAG
ATGGCGGTGATGAATATTCTTCTCATTTCAGCCCAGAACATTCAACTAAA
GAACATTATGATAGAACGTTGTCATATGTGGATAATATAAAAACACCAGA
AATCTCTCAGATTATTAGTGAACATTCCTTTAGGCCAACAAGAGTTAGTA
CCCCATCAAACAGTTTGAATACCTCTCATAAATCATCACCGATCAAATCC
CCAAAACATCAAATGGTTTCTGAGGCCATTCAAACAATTTGCGAGAATGC
TGTTCAAACAGAAGAAGATAAAGAAATACAGACTATCCCTGTGCAGTCTG
GCACCCAGACACCAAAATCACCAAGAAAACCCCCATCAGATACCAGTGCA
GGGTCAATAACATCTGTATTTGATGAATCACAAAGCGATACTCAAGCTCA
TTCCTTTAGTGACGGCATGTGGCTTTATGATCGTTCTGAAGGTGAAATGG
CACCTTATCAAATCGAAACAGTTTCTCGATGGAAATTGGGCAATTGTCCA
GAAAATCCCCTTTGGTACAATGGCACCGATGACATTATCAATGTTGATCG
ACAAAGTATTGCAAGTTTCGGGGAGTTTACAAAACCGACGTTGCCTGGTT
TGATTCCAACTCTGGACCCGGTTTTGCAGTTAAAAACTCTTCCGTTTAAA
CCTTCATACGAAACTCCAGACATTAATAAGGATGATGATTTTATGTACAT
GAGCCATAGACAACAATCTTTTCCCACTAACTTTAAATCGCTCGGTGTTT
CCAGAGATTCCAAGTCGAAATTTCCAACTCCTCAAAATGACCAAATGTCT
TCAGGGAATTTCAATCCAAGTGAAGGAGAAATCAATGACAAAGAAGACGG
CAATGATTACGTTAGCGAGGAAGAAGATAAAGAACCTGATAACTTCAATT
ATCGGTCATCGGAAGACTTTCGACCAGAATCTCCATTTTTAATGAATGTC
AAACAATCTAAAGGATCCGGGAGATCTGATACCATCGACCCTGTTAGAGA
TAGCCAATCTGATAGAATGCACATTGACGTCAATAAATATCTTCGAGCCT
CTAAGCAAAGTCATTCCGACATGAGTGATACTGCTCATTTGATGAGTACC
AATCAAATTGTGGAAAATGCGTTCCGTGGAGGCGATTCGGCGGATTTCGA
AGGATATAGTGACTATGGAAATGATCGTCATATCGGGGATAATCCCAATG
CCAACAAACAGACCGTTAAAATCCCGACTCTAGGAACCGTGGAGCAGTCA
AACCCCTCAATCAGCGATGATAACTTAGAAATTATAAACATTTAAATCAA
AAATATTTATATGAAAATGTATTTGTTAAATATATCCAATGATTTGTTGC
TTATATGAATGATTACGAAATATATGATGTAATGAC
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protein sequence of SMED30017463-orf-1

>SMED30017463-orf-1 ID=SMED30017463-orf-1|Name=SMED30017463-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1368bp
MISYDNYENQNMFQDQYEIQSDSNSKDELTLITGNNLTERAQSLIDDDVG
SNTNLDFIPVRDRCSKNKQSNSADEIEIIDVPIKIQSYEDKSKIYKCEDS
NEGWKNMNYKRNNANRDVDEYDLEGNRYGFADSNRYCDELLHTVRHIAQP
SVTLTKHENRRLDEPCQHRNYQPVFNQMNEPSSPSCYSPVTTDIGRQITQ
LARRIESLGNISRICQDDIENYSVIPKCDTSYCSPDNLIAMPHQESHISD
NYSVKAARKLDEIGRLQKKQEAMQNRLVDLAKKLNDSTRDLTRIPSKPIL
NEEPVTESDYFHGKVAKPSKKSSKKLKQKNRKSITKPHQSSFLKNILNDA
NEGKTTELPLLSSSYCLHNDLSMGTAESGISESTSKYLSDELANIRQEFR
TQVEEIRKLLSERYQQSIEQPKKPEIVQQPYELLSDQIAKSVQKLSRDNS
PSLKNITNINNNYNSECPLDKYLIKSNKIEELPSLHSEIVPSLRPGILPS
VARAIKSKQRMLSDPVEAEKFLIELANRRANLENNLRCLERTKEKEFLFS
ILERLQGDENNVDLARIQAMVDDTIASINPVVKFEVKQEYLLWIERRKEQ
REQQLIAEEEEKQFINKLKSTKQTKLQRPVHHHPLPSASAINGKPWICKK
YLSVRSKVDSGVKKQPEIPISRVARPQSPSRHTNLPSASTHATWSNRRRY
RSPYLHFINDVKLAKKSINPLGDGTRKIPIITHTETKSVKRRSRSSPQRQ
NDDLILMSVKQYSLDYKEDEEQIVPIEKPQLVAKSTSPRTISVPESTSTV
SRNSYDNKHDTNQSNKNWATQTQNQSHYSEPAPPSPSLSEKGTGFQLKSK
PSLKPREERVGTYVPMDPTETTKLVSHPVHVMLSKPQPVAEPDINGLRLL
IERALLDQIQIPSPGASYEESETVTRVGNVATPLPTDGGDEYSSHFSPEH
STKEHYDRTLSYVDNIKTPEISQIISEHSFRPTRVSTPSNSLNTSHKSSP
IKSPKHQMVSEAIQTICENAVQTEEDKEIQTIPVQSGTQTPKSPRKPPSD
TSAGSITSVFDESQSDTQAHSFSDGMWLYDRSEGEMAPYQIETVSRWKLG
NCPENPLWYNGTDDIINVDRQSIASFGEFTKPTLPGLIPTLDPVLQLKTL
PFKPSYETPDINKDDDFMYMSHRQQSFPTNFKSLGVSRDSKSKFPTPQND
QMSSGNFNPSEGEINDKEDGNDYVSEEEDKEPDNFNYRSSEDFRPESPFL
MNVKQSKGSGRSDTIDPVRDSQSDRMHIDVNKYLRASKQSHSDMSDTAHL
MSTNQIVENAFRGGDSADFEGYSDYGNDRHIGDNPNANKQTVKIPTLGTV
EQSNPSISDDNLEIINI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000096ventral epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000136whole organism
PLANA:0000418head
PLANA:0002032epidermal cell
PLANA:0002063non-ciliated epidermis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 522..542
NoneNo IPR availableCOILSCoilCoilcoord: 392..412
NoneNo IPR availableCOILSCoilCoilcoord: 256..283
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 777..867
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1188..1234
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 981..1006
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 783..833
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 317..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1212..1229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 310..338
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 673..694
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 676..694
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1022..1054
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 733..748
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1188..1211
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 732..754