Protein disulfide-isomerase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30017430 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Protein disulfide-isomerase vs. Ensembl Human
Match: P4HB (prolyl 4-hydroxylase subunit beta [Source:HGNC Symbol;Acc:HGNC:8548]) HSP 1 Score: 506.908 bits (1304), Expect = 3.423e-172 Identity = 247/449 (55.01%), Postives = 333/449 (74.16%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDE-MVDFEGDRTLETFKKFIDS 1928
V+ L SNF E L + + LVEF+APWCGHCKALAPEYA AA KLK + S I+LAKVDAT E+DLA ++ ++GYPT+KFF++G +Y GR AD I++WL+K+TGP A TL A++L+ES +V V+GFFKD S +AK F A++ DD+ FG+TS VF ++++ D +VLFKKFDEGRN+F G +++ FI QLP+VIEF ++TA KIFGG IK H+L F+ K Y F+T+A+ F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ +++E + F L+GK+KPH+M+QE+PEDWDK PVKVLVGKNF+ VA D+ K VFVEFYAPWCGHCKQLAPIWD+LGETYKD+ +IVIAKMDSTANEVE V++ SFPT+K+FP +D ++D+ G+RTL+ FKKF++S
Sbjct: 26 VLVLRKSNFAE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKD-GVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLES 472 HSP 2 Score: 112.079 bits (279), Expect = 3.260e-25 Identity = 55/106 (51.89%), Postives = 70/106 (66.04%), Query Frame = 3
Query: 174 EEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
EE+ VLVL SNF E + +KY+LVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q YG++G+PTIK F SPK+Y R A IVN
Sbjct: 22 EEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVN 127 HSP 3 Score: 73.559 bits (179), Expect = 7.130e-13 Identity = 39/94 (41.49%), Postives = 50/94 (53.19%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSK-HSPKDYQGERT 461
V VL NF +V ++ K V VEFYAPWCGHC+ LAP W K K + MD ++ + + FPT+K F S + DY GERT
Sbjct: 369 VKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERT 462
BLAST of Protein disulfide-isomerase vs. Ensembl Human
Match: P4HB (prolyl 4-hydroxylase subunit beta [Source:HGNC Symbol;Acc:HGNC:8548]) HSP 1 Score: 444.506 bits (1142), Expect = 1.580e-148 Identity = 221/449 (49.22%), Postives = 302/449 (67.26%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDE-MVDFEGDRTLETFKKFIDS 1928
V+ L SNF E L + + LVEF+APWCGHCKALAPEYA AA KLK + S I+LAKVDAT E+DLA ++ ++GYPT+KFF++G +Y G +D+ AK +++ + + DD+ FG+TS VF ++++ D +VLFKKFDEGRN+F G +++ FI QLP+VIEF ++TA KIFGG IK H+L F+ K Y F+T+A+ F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ +++E + F L+GK+KPH+M+QE+PEDWDK PVKVLVGKNF+ VA D+ K VFVEFYAPWCGHCKQLAPIWD+LGETYKD+ +IVIAKMDSTANEVE V++ SFPT+K+FP +D ++D+ G+RTL+ FKKF++S
Sbjct: 26 VLVLRKSNFAE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTGDVESDS---------------------AKQFLQAAEAI-----------------------DDIPFGITSNSDVFSKYQLDKD-GVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLES 428 HSP 2 Score: 106.686 bits (265), Expect = 1.139e-23 Identity = 51/98 (52.04%), Postives = 67/98 (68.37%), Query Frame = 3
Query: 174 EEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGE 455
EE+ VLVL SNF E + +KY+LVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q YG++G+PTIK F SPK+Y G+
Sbjct: 22 EEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTGD 119 HSP 3 Score: 73.559 bits (179), Expect = 6.463e-13 Identity = 39/94 (41.49%), Postives = 50/94 (53.19%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSK-HSPKDYQGERT 461
V VL NF +V ++ K V VEFYAPWCGHC+ LAP W K K + MD ++ + + FPT+K F S + DY GERT
Sbjct: 325 VKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERT 418 HSP 4 Score: 65.4698 bits (158), Expect = 2.228e-10 Identity = 41/115 (35.65%), Postives = 68/115 (59.13%), Query Frame = 3
Query: 1596 PEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQ-LGETYKDNTDIVIAKMDST--ANEVEGVRIQSFPTIKYFPK-DSDEMVDFEGDRTLETFKKFIDS 1928
PE+ D V VL NF + A K + VEFYAPWCGHCK LAP + + G+ + ++I +AK+D+T ++ + ++ +PTIK+F D+ ++ GD ++ K+F+ +
Sbjct: 20 PEEEDH--VLVLRKSNF-AEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTGDVESDSAKQFLQA 131
BLAST of Protein disulfide-isomerase vs. Ensembl Human
Match: PDIA2 (protein disulfide isomerase family A member 2 [Source:HGNC Symbol;Acc:HGNC:14180]) HSP 1 Score: 391.349 bits (1004), Expect = 4.305e-127 Identity = 190/447 (42.51%), Postives = 290/447 (64.88%), Query Frame = 3
Query: 627 MVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK-------DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKP-DDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKD-SDEMVDFEGDRTLETFKKFIDSN 1931
+ L+ LVEF+APWCGHC+ALAPEY+ AA L ++ ++ LAKVD + +LA +F + YPTLKFF++G +Y G R A+ I WL ++ GP A L+ A+ALI +D+VV+GFF+D F ++A D+ FG+T + +F +F ++ D ++VLFKKFDEGR DF VD+ D+ F+ + + +V EFN +T+ KIF I H+L F+++ + EL F +A +F GQVLFV+++ DN+ VL++FG+ P+ R++ LE K+ P D +++ ++ F +VL+G+VKP++++QEIP DWD+ PVK LVGKNF+ VA D++K VFV+FYAPWC HCK++AP W+ L E Y+D+ DI+IA++D+TANE++ + FPT+KYFP ++++++ R LETF KF+D+
Sbjct: 54 LALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDGPAQRELAEEFGVTEYPTLKFFRNGNRTHPEEYTGPRDAEGIAEWLRRRVGPSAMRLEDEAAAQALIGGRDLVVIGFFQDLQDEDVATFLALAQDALDMTFGLTDRPRLFQQFGLTKD-TVVLFKKFDEGRADFP---VDEELGLDLGDLSRFLVTHSMRLVTEFNSQTSAKIFAARILNHLLLFVNQTLAAHRELLAGFGEAAPRFRGQVLFVVVDV-AADNEHVLQYFGLKAEAAPTLRLVNLETTK-KYAPVDGGPVTAASITAFCHAVLNGQVKPYLLSQEIPPDWDQRPVKTLVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDIIIAELDATANELDAFAVHGFPTLKYFPAGPGRKVIEYKSTRDLETFSKFLDNG 494 HSP 2 Score: 87.8113 bits (216), Expect = 2.271e-17 Identity = 43/104 (41.35%), Postives = 60/104 (57.69%), Query Frame = 3
Query: 180 EGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIF--GKSKHSPKDYQGERTAGSIV 476
+G+LVL+ + ++ +LVEFYAPWCGHCQ+LAPE+ KAA L VV + VD L + +G+ +PT+K F G H P++Y G R A I
Sbjct: 42 DGILVLSRHTLGLALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDGPAQRELAEEFGVTEYPTLKFFRNGNRTH-PEEYTGPRDAEGIA 144 HSP 3 Score: 72.4034 bits (176), Expect = 1.690e-12 Identity = 40/106 (37.74%), Postives = 63/106 (59.43%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLE 902
V L NF+++ + V+F+APWC HCK +AP + + AEK ++ II +A++DAT N+L F + G+PTLK+F G KV++Y R +T +L+
Sbjct: 390 VKTLVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDII-IAELDATA-NEL-DAFAVHGFPTLKYFPAGPGRKVIEYKSTRDLETFSKFLD 492 HSP 4 Score: 60.4622 bits (145), Expect = 7.566e-9 Identity = 34/109 (31.19%), Postives = 53/109 (48.62%), Query Frame = 3
Query: 123 SIVLFCFAL--AQCNADITEEE--------GVLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF 416
SI FC A+ Q + +E V L NF +V ++ K V V+FYAPWC HC+ +AP W+ A + + ++D ++ + + GFPT+K F
Sbjct: 359 SITAFCHAVLNGQVKPYLLSQEIPPDWDQRPVKTLVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDIIIAELDATANELDAFAVHGFPTLKYF 467 HSP 5 Score: 56.225 bits (134), Expect = 1.683e-7 Identity = 26/79 (32.91%), Postives = 45/79 (56.96%), Query Frame = 3
Query: 1626 VLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIV-IAKMDSTANE--VEGVRIQSFPTIKYF 1853
+++ ++ +A + A+ VEFYAPWCGHC+ LAP + + + +V +AK+D A E + +PT+K+F
Sbjct: 45 LVLSRHTLGLALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDGPAQRELAEEFGVTEYPTLKFF 123
BLAST of Protein disulfide-isomerase vs. Ensembl Human
Match: PDIA2 (protein disulfide isomerase family A member 2 [Source:HGNC Symbol;Acc:HGNC:14180]) HSP 1 Score: 389.423 bits (999), Expect = 2.014e-126 Identity = 191/447 (42.73%), Postives = 290/447 (64.88%), Query Frame = 3
Query: 627 MVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK-------DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKP-DDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKD-SDEMVDFEGDRTLETFKKFIDSN 1931
+ L+ LVEF+APWCGHC+ALAPEY+ AA L ++ ++ LAKVD + +LA +F + YPTLKFF++G +Y G R A+ I WL ++ GP A L+ A+ALI +D+VV+GFF+D+ A A A D+ FG+T + +F +F ++ D ++VLFKKFDEGR DF VD+ D+ F+ + + +V EFN +T+ KIF I H+L F+++ + EL F +A +F GQVLFV+++ DN+ VL++FG+ P+ R++ LE K+ P D +++ ++ F +VL+G+VKP++++QEIP DWD+ PVK LVGKNF+ VA D++K VFV+FYAPWC HCK++AP W+ L E Y+D+ DI+IA++D+TANE++ + FPT+KYFP ++++++ R LETF KF+D+
Sbjct: 54 LALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDGPAQRELAEEFGVTEYPTLKFFRNGNRTHPEEYTGPRDAEGIAEWLRRRVGPSAMRLEDEAAAQALIGGRDLVVIGFFQDEDVATFLALAQDAL---DMTFGLTDRPRLFQQFGLTKD-TVVLFKKFDEGRADFP---VDEELGLDLGDLSRFLVTHSMRLVTEFNSQTSAKIFAARILNHLLLFVNQTLAAHRELLAGFGEAAPRFRGQVLFVVVDV-AADNEHVLQYFGLKAEAAPTLRLVNLETTK-KYAPVDGGPVTAASITAFCHAVLNGQVKPYLLSQEIPPDWDQRPVKTLVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDIIIAELDATANELDAFAVHGFPTLKYFPAGPGRKVIEYKSTRDLETFSKFLDNG 491 HSP 2 Score: 87.8113 bits (216), Expect = 2.283e-17 Identity = 43/104 (41.35%), Postives = 60/104 (57.69%), Query Frame = 3
Query: 180 EGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIF--GKSKHSPKDYQGERTAGSIV 476
+G+LVL+ + ++ +LVEFYAPWCGHCQ+LAPE+ KAA L VV + VD L + +G+ +PT+K F G H P++Y G R A I
Sbjct: 42 DGILVLSRHTLGLALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDGPAQRELAEEFGVTEYPTLKFFRNGNRTH-PEEYTGPRDAEGIA 144 HSP 3 Score: 72.4034 bits (176), Expect = 1.768e-12 Identity = 40/106 (37.74%), Postives = 63/106 (59.43%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLE 902
V L NF+++ + V+F+APWC HCK +AP + + AEK ++ II +A++DAT N+L F + G+PTLK+F G KV++Y R +T +L+
Sbjct: 387 VKTLVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDII-IAELDATA-NEL-DAFAVHGFPTLKYFPAGPGRKVIEYKSTRDLETFSKFLD 489 HSP 4 Score: 60.4622 bits (145), Expect = 7.999e-9 Identity = 34/109 (31.19%), Postives = 53/109 (48.62%), Query Frame = 3
Query: 123 SIVLFCFAL--AQCNADITEEE--------GVLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF 416
SI FC A+ Q + +E V L NF +V ++ K V V+FYAPWC HC+ +AP W+ A + + ++D ++ + + GFPT+K F
Sbjct: 356 SITAFCHAVLNGQVKPYLLSQEIPPDWDQRPVKTLVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDIIIAELDATANELDAFAVHGFPTLKYF 464 HSP 5 Score: 56.225 bits (134), Expect = 1.620e-7 Identity = 26/79 (32.91%), Postives = 45/79 (56.96%), Query Frame = 3
Query: 1626 VLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIV-IAKMDSTANE--VEGVRIQSFPTIKYF 1853
+++ ++ +A + A+ VEFYAPWCGHC+ LAP + + + +V +AK+D A E + +PT+K+F
Sbjct: 45 LVLSRHTLGLALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDGPAQRELAEEFGVTEYPTLKFF 123
BLAST of Protein disulfide-isomerase vs. Ensembl Human
Match: PDIA4 (protein disulfide isomerase family A member 4 [Source:HGNC Symbol;Acc:HGNC:30167]) HSP 1 Score: 321.242 bits (822), Expect = 9.168e-99 Identity = 223/606 (36.80%), Postives = 323/606 (53.30%), Query Frame = 3
Query: 183 GVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAA---TLKSVEEAKALIESKDVVVVGFFKDQX-XXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVL----FKKFDEGRN---DFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETS-----AKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVR--IQSFPTIKYFPK-DSDEMVDFE-GDRTLETFKKFIDSN 1931
GVLVL ++NF+ + VL+EFYAPWCGHC+ APE++K A LK + V +D S L + + G+PTIKI K + DY+G RT IV A ++E+ + + + LT NFDE V+ + LVEF+APWCGHCK LAPEY AA++L +++ I LAKVDAT E DLA +F + GYPTLK F+ G+ DY G R I+ ++ +++GPP+ TLK V+E L + DV+++G FK ++ A + ++ + ++D F T + KVS +V+ F+ E R+ D G D I+ F+ KY LP+V K + + + V+ + S QF S AK F + F I DEED ++ G++++ I L++ KF + S+ L FV + GK+KP + +Q +P++ +K PVKV+VGK FDS+ D K V +EFYAPWCGHCKQL P+++ L + YK +VIAKMD+TAN+V R ++ FPTI + P D V FE GDR LE KFI+ +
Sbjct: 63 GVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAV--DYEGSRTQEEIV-AKVREVSQ---------------------PDWTPPPEVTLVLTKENFDE-VVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQE--FLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRY--TRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDF-PEYTFAI--ADEEDYAGEVKDLGLSESGEDVNAAI-LDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKN-NKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEH 634 HSP 2 Score: 92.8189 bits (229), Expect = 8.516e-19 Identity = 46/108 (42.59%), Postives = 65/108 (60.19%), Query Frame = 3
Query: 180 EGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL---KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKE 494
E LVLT NF+EV+ +LVEFYAPWCGHC+ LAPE++KAA L + + VD + L + + + G+PT+KIF K + P DY G R IV+ +++
Sbjct: 177 EVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGR--PYDYNGPREKYGIVDYMIEQ 282 HSP 3 Score: 80.1073 bits (196), Expect = 8.304e-15 Identity = 45/100 (45.00%), Postives = 60/100 (60.00%), Query Frame = 3
Query: 1614 NPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKD-NTDIVIAKMDSTANEVEGVR--IQSFPTIKYFPKDSDEMVDFEGDRTLE 1904
N V VL NFD+ DK V +EFYAPWCGHCKQ AP ++++ KD + I +AK+D+T+ V R + +PTIK K + VD+EG RT E
Sbjct: 62 NGVLVLNDANFDNFVADKD-TVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKK--GQAVDYEGSRTQE 158
BLAST of Protein disulfide-isomerase vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770]) HSP 1 Score: 517.694 bits (1332), Expect = 4.247e-177 Identity = 257/447 (57.49%), Postives = 337/447 (75.39%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
+V+ LT NFDE V+ E LVEF+APWCGHCK+LAPEYA AA +LKE+ S IKL K+DATV +++SKF+++GYPTLK F++GK +Y GGR D+II+WL+KKTGP A L + K L ES DVVV+G+FKD S AK F VA DDV FG++++ AV E ++ + IVLFKKFD+GR F ++T+I +L +V EF QETA IFGG IK H L F+SK+S ++A+L+ +F+ +AK+F G+VLFV INTD E+N R++EFFG+ K + P+ R+I LE+DM KFKPD I++EN++ F Q+ LDG VKPH+M+++IPEDWDKNPVK+LVGKNF+ VARD +K V VEFYAPWCGHCKQLAP WD+LGE + D+ IVIAKMDST NEVE V+IQSFPTIK+FP S+++VD+ GDRT+E F KF+++N
Sbjct: 24 NVIVLTKDNFDE-VINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGYFKDTTSDDAKTFLEVAAGIDDVPFGISTEDAVKSEIELKGE-GIVLFKKFDDGRVAFDEKLTQDGLKTWIQANRLALVSEFTQETASVIFGGEIKSHNLLFVSKESSEFAKLEQEFKNAAKQFKGKVLFVYINTDVEENARIMEFFGLKKDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNPVKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETN 468 HSP 2 Score: 115.931 bits (289), Expect = 5.218e-27 Identity = 56/118 (47.46%), Postives = 77/118 (65.25%), Query Frame = 3
Query: 132 LFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
LF L A I EEE V+VLT NF+EVI N+++LVEFYAPWCGHC+SLAPE+ KAAT LK +K+G +D + + + ++G+PT+K+F K P++Y G R SI+
Sbjct: 7 LFFLVLGASAAVIEEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGK--PQEYNGGRDHDSII 122 HSP 3 Score: 77.411 bits (189), Expect = 1.310e-14 Identity = 40/93 (43.01%), Postives = 48/93 (51.61%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIEKN-KYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V +L NF +V N K VLVEFYAPWCGHC+ LAP W K + MD + + IQ FPTIK F + DY G+RT
Sbjct: 365 VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRT 457
BLAST of Protein disulfide-isomerase vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770]) HSP 1 Score: 517.694 bits (1332), Expect = 4.247e-177 Identity = 257/447 (57.49%), Postives = 337/447 (75.39%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
+V+ LT NFDE V+ E LVEF+APWCGHCK+LAPEYA AA +LKE+ S IKL K+DATV +++SKF+++GYPTLK F++GK +Y GGR D+II+WL+KKTGP A L + K L ES DVVV+G+FKD S AK F VA DDV FG++++ AV E ++ + IVLFKKFD+GR F ++T+I +L +V EF QETA IFGG IK H L F+SK+S ++A+L+ +F+ +AK+F G+VLFV INTD E+N R++EFFG+ K + P+ R+I LE+DM KFKPD I++EN++ F Q+ LDG VKPH+M+++IPEDWDKNPVK+LVGKNF+ VARD +K V VEFYAPWCGHCKQLAP WD+LGE + D+ IVIAKMDST NEVE V+IQSFPTIK+FP S+++VD+ GDRT+E F KF+++N
Sbjct: 24 NVIVLTKDNFDE-VINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGYFKDTTSDDAKTFLEVAAGIDDVPFGISTEDAVKSEIELKGE-GIVLFKKFDDGRVAFDEKLTQDGLKTWIQANRLALVSEFTQETASVIFGGEIKSHNLLFVSKESSEFAKLEQEFKNAAKQFKGKVLFVYINTDVEENARIMEFFGLKKDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNPVKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETN 468 HSP 2 Score: 115.931 bits (289), Expect = 5.218e-27 Identity = 56/118 (47.46%), Postives = 77/118 (65.25%), Query Frame = 3
Query: 132 LFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
LF L A I EEE V+VLT NF+EVI N+++LVEFYAPWCGHC+SLAPE+ KAAT LK +K+G +D + + + ++G+PT+K+F K P++Y G R SI+
Sbjct: 7 LFFLVLGASAAVIEEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGK--PQEYNGGRDHDSII 122 HSP 3 Score: 77.411 bits (189), Expect = 1.310e-14 Identity = 40/93 (43.01%), Postives = 48/93 (51.61%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIEKN-KYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V +L NF +V N K VLVEFYAPWCGHC+ LAP W K + MD + + IQ FPTIK F + DY G+RT
Sbjct: 365 VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRT 457
BLAST of Protein disulfide-isomerase vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770]) HSP 1 Score: 465.307 bits (1196), Expect = 1.661e-157 Identity = 233/447 (52.13%), Postives = 306/447 (68.46%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
+V+ LT NFDE V+ E LVEF+APWCGHCK+LAPEYA AA +LKE+ S IKL K+DATV +++SKF+++GYPTLK F++GK +Y GGR D+II+WL+KKTGP A L + K L ES DVVV+G+ FK ++ D D +T+I +L +V EF QETA IFGG IK H L F+SK+S ++A+L+ +F+ +AK+F G+VLFV INTD E+N R++EFFG+ K + P+ R+I LE+DM KFKPD I++EN++ F Q+ LDG VKPH+M+++IPEDWDKNPVK+LVGKNF+ VARD +K V VEFYAPWCGHCKQLAP WD+LGE + D+ IVIAKMDST NEVE V+IQSFPTIK+FP S+++VD+ GDRT+E F KF+++N
Sbjct: 24 NVIVLTKDNFDE-VINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGY-----------------------------------FKDTTSD----------------------DAKTWIQANRLALVSEFTQETASVIFGGEIKSHNLLFVSKESSEFAKLEQEFKNAAKQFKGKVLFVYINTDVEENARIMEFFGLKKDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNPVKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETN 412 HSP 2 Score: 115.546 bits (288), Expect = 3.887e-27 Identity = 56/118 (47.46%), Postives = 77/118 (65.25%), Query Frame = 3
Query: 132 LFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
LF L A I EEE V+VLT NF+EVI N+++LVEFYAPWCGHC+SLAPE+ KAAT LK +K+G +D + + + ++G+PT+K+F K P++Y G R SI+
Sbjct: 7 LFFLVLGASAAVIEEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGK--PQEYNGGRDHDSII 122 HSP 3 Score: 77.0258 bits (188), Expect = 1.415e-14 Identity = 40/93 (43.01%), Postives = 48/93 (51.61%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIEKN-KYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V +L NF +V N K VLVEFYAPWCGHC+ LAP W K + MD + + IQ FPTIK F + DY G+RT
Sbjct: 309 VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRT 401
BLAST of Protein disulfide-isomerase vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770]) HSP 1 Score: 465.307 bits (1196), Expect = 1.661e-157 Identity = 233/447 (52.13%), Postives = 306/447 (68.46%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
+V+ LT NFDE V+ E LVEF+APWCGHCK+LAPEYA AA +LKE+ S IKL K+DATV +++SKF+++GYPTLK F++GK +Y GGR D+II+WL+KKTGP A L + K L ES DVVV+G+ FK ++ D D +T+I +L +V EF QETA IFGG IK H L F+SK+S ++A+L+ +F+ +AK+F G+VLFV INTD E+N R++EFFG+ K + P+ R+I LE+DM KFKPD I++EN++ F Q+ LDG VKPH+M+++IPEDWDKNPVK+LVGKNF+ VARD +K V VEFYAPWCGHCKQLAP WD+LGE + D+ IVIAKMDST NEVE V+IQSFPTIK+FP S+++VD+ GDRT+E F KF+++N
Sbjct: 24 NVIVLTKDNFDE-VINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGY-----------------------------------FKDTTSD----------------------DAKTWIQANRLALVSEFTQETASVIFGGEIKSHNLLFVSKESSEFAKLEQEFKNAAKQFKGKVLFVYINTDVEENARIMEFFGLKKDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNPVKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETN 412 HSP 2 Score: 115.546 bits (288), Expect = 3.887e-27 Identity = 56/118 (47.46%), Postives = 77/118 (65.25%), Query Frame = 3
Query: 132 LFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
LF L A I EEE V+VLT NF+EVI N+++LVEFYAPWCGHC+SLAPE+ KAAT LK +K+G +D + + + ++G+PT+K+F K P++Y G R SI+
Sbjct: 7 LFFLVLGASAAVIEEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGK--PQEYNGGRDHDSII 122 HSP 3 Score: 77.0258 bits (188), Expect = 1.415e-14 Identity = 40/93 (43.01%), Postives = 48/93 (51.61%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIEKN-KYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V +L NF +V N K VLVEFYAPWCGHC+ LAP W K + MD + + IQ FPTIK F + DY G+RT
Sbjct: 309 VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRT 401
BLAST of Protein disulfide-isomerase vs. Ensembl Celegans
Match: pdi-1 (Protein disulfide-isomerase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q17967]) HSP 1 Score: 366.696 bits (940), Expect = 1.355e-118 Identity = 197/451 (43.68%), Postives = 286/451 (63.41%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDI------RTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFID 1925
+V+ LT+SNF+E + E LV+F+APWC HCK+LAP+Y AA+ LKE+ S IKLAKVDAT LASKF+++GYPT+ +FK GK Y GGR I+ W++KK+GP T++SVE+ + L VVV+G+FKD S AA + VADS DD F V V ++ +D + L + +G + T + +I + ++ Y+L V EF E+A +I GG +KK I K + E +F AKKF +++FV+++ D E+N R+LEF G+ +TP+ R++ L D + KFKP + E+ F S L+GK + Q++PEDW+ PVKVLV NF+ +A D++K VFV+FYAPWCGHCKQL P+WD+L E Y+ N ++VIAK+D+T NE+ V++ SFPT+K +P S VD++GDR LE F++F++
Sbjct: 24 NVLVLTESNFEETI-NGNEFVLVKFYAPWCVHCKSLAPKYDEAADLLKEEGSDIKLAKVDATENQALASKFEVRGYPTILYFKSGKPTKYTGGRATAQIVDWVKKKSGPTVTTVESVEQLEELKGKTRVVVLGYFKDAKSDAATIYNEVADSVDDAFFAVAGSAEVAAAASLN-EDGVALIRT--DGDDSETSTIAEAEITNTIALKQWLHAYKLSAVTEFTHESAQEIVGGDLKKFHFLIIRKSDSSFDETIAKFTEVAKKFRAKIVFVLLDVDVEENARILEFLGVDAKNTPANRIVSLADQVEKFKPQEG----EDFEAFTNSYLEGKSAQDLKAQDLPEDWNALPVKVLVASNFNEIALDETKTVFVKFYAPWCGHCKQLVPVWDELAEKYESNPNVVIAKLDATLNELADVKVNSFPTLKLWPAGSSTPVDYDGDRNLEKFEEFVN 466 HSP 2 Score: 99.3673 bits (246), Expect = 1.466e-21 Identity = 51/123 (41.46%), Postives = 71/123 (57.72%), Query Frame = 3
Query: 120 LSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVN 479
LS+ +A A + + E VLVLT SNF E I N++VLV+FYAPWC HC+SLAP++ +AA LK +K+ VD + +L + ++G+PTI F K P Y G R IV+
Sbjct: 3 LSVSFIFLLVASIGAVVADSENVLVLTESNFEETINGNEFVLVKFYAPWCVHCKSLAPKYDEAADLLKEEGSDIKLAKVDATENQALASKFEVRGYPTILYFKSGK--PTKYTGGRATAQIVD 123 HSP 3 Score: 73.559 bits (179), Expect = 2.109e-13 Identity = 35/92 (38.04%), Postives = 51/92 (55.43%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGER 458
V VL SNFNE+ +++ K V V+FYAPWCGHC+ L P W + A + V +D + + FPT+K++ +P DY G+R
Sbjct: 365 VKVLVASNFNEIALDETKTVFVKFYAPWCGHCKQLVPVWDELAEKYESNPNVVIAKLDATLNELADVKVNSFPTLKLWPAGSSTPVDYDGDR 456
BLAST of Protein disulfide-isomerase vs. Ensembl Fly
Match: Pdi (gene:FBgn0286818 transcript:FBtr0346644) HSP 1 Score: 490.73 bits (1262), Expect = 1.868e-166 Identity = 239/446 (53.59%), Postives = 330/446 (73.99%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
V+ T NF +++ +E LVEF+APWCGHCKALAPEYA AA++L EK S IKLAKVDATVE +LA ++ ++GYPTLKFF+ G ++Y GGR A II+W+ KKTGPPA L SV +A+ ++ ++ ++GFFKD S AK F VA++ D VFGV+S V +++ + D+ +VLFK FD+ ++ F G +++++ F LP++++FN E+A KIFGGSIK H+L F+S++ + + AKK+ +LFV I++DEED+ R+ EFFGM K + P+ R+I+LE+DM K+KP+ +S+E + F++ LDGK+K H+++QE+PEDWDKNPVKVLV NF+SVA DKSK+V VEFYAPWCGHCKQLAPI+DQL E YKDN DIVIAKMDSTANE+E ++I SFPTIKYF K+ ++++DF DRTL+ F KF+D+N
Sbjct: 29 VLVATVDNFKQLIADNE-FVLVEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSGSPVEYSGGRQAADIIAWVTKKTGPPAKDLTSVADAEQFLKDNEIAIIGFFKDLESEEAKTFTKVANALDSFVFGVSSNADVIAKYE-AKDNGVVLFKPFDDKKSVFEGELNEENLKKFAQVQSLPLIVDFNHESASKIFGGSIKSHLLFFVSREGGHIEKYVDPLKEIAKKYRDDILFVTISSDEEDHTRIFEFFGMNKEEVPTIRLIKLEEDMAKYKPESDDLSAETIEAFLKKFLDGKLKQHLLSQELPEDWDKNPVKVLVSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNEDIVIAKMDSTANELESIKISSFPTIKYFRKEDNKVIDFNLDRTLDDFVKFLDAN 472 HSP 2 Score: 111.694 bits (278), Expect = 1.688e-25 Identity = 56/128 (43.75%), Postives = 77/128 (60.16%), Query Frame = 3
Query: 111 MKYLSIVLF---CFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL---KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
MK+L LF + A A++ EEGVLV T NF ++I N++VLVEFYAPWCGHC++LAPE+ KAA L + +K+ VD L + Y ++G+PT+K F SP +Y G R A I+
Sbjct: 1 MKFLICALFLAASYVAASAEAEVKVEEGVLVATVDNFKQLIADNEFVLVEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSG--SPVEYSGGRQAADII 126 HSP 3 Score: 75.0998 bits (183), Expect = 9.198e-14 Identity = 41/98 (41.84%), Postives = 55/98 (56.12%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
V VL +SNF V ++K+K VLVEFYAPWCGHC+ LAP + + A K + MD ++ + I FPTIK F K + D+ +RT V
Sbjct: 369 VKVLVSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNEDIVIAKMDSTANELESIKISSFPTIKYFRKEDNKVIDFNLDRTLDDFV 466
BLAST of Protein disulfide-isomerase vs. Ensembl Fly
Match: Pdi (gene:FBgn0286818 transcript:FBtr0075648) HSP 1 Score: 490.73 bits (1262), Expect = 1.868e-166 Identity = 239/446 (53.59%), Postives = 330/446 (73.99%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
V+ T NF +++ +E LVEF+APWCGHCKALAPEYA AA++L EK S IKLAKVDATVE +LA ++ ++GYPTLKFF+ G ++Y GGR A II+W+ KKTGPPA L SV +A+ ++ ++ ++GFFKD S AK F VA++ D VFGV+S V +++ + D+ +VLFK FD+ ++ F G +++++ F LP++++FN E+A KIFGGSIK H+L F+S++ + + AKK+ +LFV I++DEED+ R+ EFFGM K + P+ R+I+LE+DM K+KP+ +S+E + F++ LDGK+K H+++QE+PEDWDKNPVKVLV NF+SVA DKSK+V VEFYAPWCGHCKQLAPI+DQL E YKDN DIVIAKMDSTANE+E ++I SFPTIKYF K+ ++++DF DRTL+ F KF+D+N
Sbjct: 29 VLVATVDNFKQLIADNE-FVLVEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSGSPVEYSGGRQAADIIAWVTKKTGPPAKDLTSVADAEQFLKDNEIAIIGFFKDLESEEAKTFTKVANALDSFVFGVSSNADVIAKYE-AKDNGVVLFKPFDDKKSVFEGELNEENLKKFAQVQSLPLIVDFNHESASKIFGGSIKSHLLFFVSREGGHIEKYVDPLKEIAKKYRDDILFVTISSDEEDHTRIFEFFGMNKEEVPTIRLIKLEEDMAKYKPESDDLSAETIEAFLKKFLDGKLKQHLLSQELPEDWDKNPVKVLVSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNEDIVIAKMDSTANELESIKISSFPTIKYFRKEDNKVIDFNLDRTLDDFVKFLDAN 472 HSP 2 Score: 111.694 bits (278), Expect = 1.688e-25 Identity = 56/128 (43.75%), Postives = 77/128 (60.16%), Query Frame = 3
Query: 111 MKYLSIVLF---CFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL---KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
MK+L LF + A A++ EEGVLV T NF ++I N++VLVEFYAPWCGHC++LAPE+ KAA L + +K+ VD L + Y ++G+PT+K F SP +Y G R A I+
Sbjct: 1 MKFLICALFLAASYVAASAEAEVKVEEGVLVATVDNFKQLIADNEFVLVEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSG--SPVEYSGGRQAADII 126 HSP 3 Score: 75.0998 bits (183), Expect = 9.198e-14 Identity = 41/98 (41.84%), Postives = 55/98 (56.12%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
V VL +SNF V ++K+K VLVEFYAPWCGHC+ LAP + + A K + MD ++ + I FPTIK F K + D+ +RT V
Sbjct: 369 VKVLVSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNEDIVIAKMDSTANELESIKISSFPTIKYFRKEDNKVIDFNLDRTLDDFV 466
BLAST of Protein disulfide-isomerase vs. Ensembl Fly
Match: ERp60 (gene:FBgn0033663 transcript:FBtr0088058) HSP 1 Score: 243.817 bits (621), Expect = 8.366e-72 Identity = 169/459 (36.82%), Postives = 256/459 (55.77%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDAT-VENDLASKFKIQGYPTLKFFKDGKV-MDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXX-XXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFK------KFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAEL-KGQFETSAKKFLGQVLFVIINTDE---EDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEG-VRIQSFPTIKYFPKDS-DEMVDFEGDRTLETFKKFI 1922
DV+EL D +F LK E LV F+APWCGHCK L PEYA AAE +K+ + IKLAKVD T + SK+ + GYPTLK F+ +V DY G R A I ++ + GP + T+++V E K +++KD + G+F D S AK F +++ FG +S+K V D K D IVL + KF+ F G + D+ TF+ + +V Q++ K F + S +K+ K + + AK+F+GQ+ F I + D+ E N+ +F G D P ++ ++ +K+ D S ENL FV+ +L +++P++ ++ IPE D PVKV V KNFD + + K +EFYAPWCGHCK+L+PI+++L E +D D+ I KMD+TAN+V ++ FPT+ + PKD+ ++ V + G R ++ F K+I
Sbjct: 23 DVLELGDDDF-ATTLKQHETTLVMFYAPWCGHCKRLKPEYAKAAEIVKDDDPPIKLAKVDCTEAGKETCSKYSVSGYPTLKIFRQDEVSQDYNGPREASGIAKYMRAQVGPASKTVRTVAELKKFLDTKDTTLFGYFSDSDSKLAKIFLKFADKNREKYRFGHSSEKEVLD--KQGETDKIVLIRAPHLSNKFESSSIKFEGS-SESDLSTFVKENFHGLVGHRTQDSV-KDFQNPLITAYYSVDYQKNPKGTNYWRNRVLKVAKEFVGQINFAIASKDDFQHELNEYGYDFVG----DKPV--VLARDEKNLKYALKD-EFSVENLQDFVEKLLANELEPYIKSEPIPESNDA-PVKVAVAKNFDDLVINNGKDTLIEFYAPWCGHCKKLSPIYEELAEKLQDE-DVAIVKMDATANDVPPEFNVRGFPTLFWLPKDAKNKPVSYNGGREVDDFLKYI 467 HSP 2 Score: 75.0998 bits (183), Expect = 9.727e-14 Identity = 40/104 (38.46%), Postives = 60/104 (57.69%), Query Frame = 3
Query: 177 EEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYS-SLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
E+ VL L + +F +++++ LV FYAPWCGHC+ L PE+ KAA +K +K+ VD + Y + G+PT+KIF + + S +DY G R A I
Sbjct: 21 EQDVLELGDDDFATTLKQHETTLVMFYAPWCGHCKRLKPEYAKAAEIVKDDDPPIKLAKVDCTEAGKETCSKYSVSGYPTLKIFRQDEVS-QDYNGPREASGIA 123 HSP 3 Score: 62.7734 bits (151), Expect = 7.312e-10 Identity = 39/106 (36.79%), Postives = 57/106 (53.77%), Query Frame = 3
Query: 186 VLVLTNSNFNE-VIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQP-YGIQGFPTIKIFGK-SKHSPKDYQGERTAGSIVNAALKE 494
V V NF++ VI K L+EFYAPWCGHC+ L+P +++ A L+ V V MD ++ P + ++GFPT+ K +K+ P Y G R + KE
Sbjct: 366 VKVAVAKNFDDLVINNGKDTLIEFYAPWCGHCKKLSPIYEELAEKLQD-EDVAIVKMDATANDVPPEFNVRGFPTLFWLPKDAKNKPVSYNGGREVDDFLKYIAKE 470
BLAST of Protein disulfide-isomerase vs. Ensembl Fly
Match: CaBP1 (gene:FBgn0025678 transcript:FBtr0345544) HSP 1 Score: 238.424 bits (607), Expect = 1.860e-70 Identity = 136/268 (50.75%), Postives = 179/268 (66.79%), Query Frame = 3
Query: 123 SIVLFCFALAQCNADITEEEGVLVLTNSNFN-EVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK-----VMDYGGGRTADTIISWLEKK 908
SI+L F + +A + +GV+ LT SNF+ EV++ + +VEFYAPWCGHCQSL PE+KK A ALKGVVKVG+V+ D S+L +G++GFPTIKIFG +K SP DY G+RTA +I AAL E+ + V+ L S G S SG DV+ELT+ NFD++VL S++ WLVEFFAPWCGHCK LAPE+A AA++LK K +KL +DAT A+++ ++GYPT+KFF G +Y GGRTA I+SW K
Sbjct: 6 SILLLAFVVGSVSAFYSPSDGVVELTPSNFDREVLKDDAIWVVEFYAPWCGHCQSLVPEYKKLAKALKGVVKVGSVNADADSTLSGQFGVRGFPTIKIFGANKKSPTDYNGQRTAKAIAEAALAEVKKKVQGVL------GGGGGSSSGGSGSSSGDDVIELTEDNFDKLVLNSDDIWLVEFFAPWCGHCKNLAPEWAKAAKELKGK---VKLGALDATAHQSKAAEYNVRGYPTIKFFPAGSKRASDAQEYDGGRTASDIVSWASDK 264 HSP 2 Score: 68.9366 bits (167), Expect = 6.411e-12 Identity = 34/95 (35.79%), Postives = 52/95 (54.74%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGV--RIQSFPTIKYFPKDSDEMVD---FEGDRT 1898
L NFD + + VEF+APWCGHCK LAP W + + K + + +D+TA++ + ++ +PTIK+FP S D ++G RT
Sbjct: 161 LTEDNFDKLVLNSDDIWLVEFFAPWCGHCKNLAPEWAKAAKELKGK--VKLGALDATAHQSKAAEYNVRGYPTIKFFPAGSKRASDAQEYDGGRT 253 HSP 3 Score: 63.5438 bits (153), Expect = 3.776e-10 Identity = 37/92 (40.22%), Postives = 45/92 (48.91%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDI--VIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRT 1898
L NFD VEFYAPWCGHC+ L P + +L + K + V A DST + GVR FPTIK F + D+ G RT
Sbjct: 30 LTPSNFDREVLKDDAIWVVEFYAPWCGHCQSLVPEYKKLAKALKGVVKVGSVNADADSTLSGQFGVR--GFPTIKIFGANKKSPTDYNGQRT 119
BLAST of Protein disulfide-isomerase vs. Ensembl Fly
Match: CaBP1 (gene:FBgn0025678 transcript:FBtr0080837) HSP 1 Score: 238.424 bits (607), Expect = 1.860e-70 Identity = 136/268 (50.75%), Postives = 179/268 (66.79%), Query Frame = 3
Query: 123 SIVLFCFALAQCNADITEEEGVLVLTNSNFN-EVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK-----VMDYGGGRTADTIISWLEKK 908
SI+L F + +A + +GV+ LT SNF+ EV++ + +VEFYAPWCGHCQSL PE+KK A ALKGVVKVG+V+ D S+L +G++GFPTIKIFG +K SP DY G+RTA +I AAL E+ + V+ L S G S SG DV+ELT+ NFD++VL S++ WLVEFFAPWCGHCK LAPE+A AA++LK K +KL +DAT A+++ ++GYPT+KFF G +Y GGRTA I+SW K
Sbjct: 6 SILLLAFVVGSVSAFYSPSDGVVELTPSNFDREVLKDDAIWVVEFYAPWCGHCQSLVPEYKKLAKALKGVVKVGSVNADADSTLSGQFGVRGFPTIKIFGANKKSPTDYNGQRTAKAIAEAALAEVKKKVQGVL------GGGGGSSSGGSGSSSGDDVIELTEDNFDKLVLNSDDIWLVEFFAPWCGHCKNLAPEWAKAAKELKGK---VKLGALDATAHQSKAAEYNVRGYPTIKFFPAGSKRASDAQEYDGGRTASDIVSWASDK 264 HSP 2 Score: 68.9366 bits (167), Expect = 6.411e-12 Identity = 34/95 (35.79%), Postives = 52/95 (54.74%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGV--RIQSFPTIKYFPKDSDEMVD---FEGDRT 1898
L NFD + + VEF+APWCGHCK LAP W + + K + + +D+TA++ + ++ +PTIK+FP S D ++G RT
Sbjct: 161 LTEDNFDKLVLNSDDIWLVEFFAPWCGHCKNLAPEWAKAAKELKGK--VKLGALDATAHQSKAAEYNVRGYPTIKFFPAGSKRASDAQEYDGGRT 253 HSP 3 Score: 63.5438 bits (153), Expect = 3.776e-10 Identity = 37/92 (40.22%), Postives = 45/92 (48.91%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDI--VIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRT 1898
L NFD VEFYAPWCGHC+ L P + +L + K + V A DST + GVR FPTIK F + D+ G RT
Sbjct: 30 LTPSNFDREVLKDDAIWVVEFYAPWCGHCQSLVPEYKKLAKALKGVVKVGSVNADADSTLSGQFGVR--GFPTIKIFGANKKSPTDYNGQRT 119
BLAST of Protein disulfide-isomerase vs. Ensembl Zebrafish
Match: p4hb (prolyl 4-hydroxylase, beta polypeptide [Source:ZFIN;Acc:ZDB-GENE-080610-1]) HSP 1 Score: 501.516 bits (1290), Expect = 2.466e-170 Identity = 246/450 (54.67%), Postives = 338/450 (75.11%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK---VMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPK-DSDEMVDFEGDRTLETFKKFIDS 1928
DV+ L SNF+E LK+ LVEF+APWCGHCKALAPEY+ AA LK + S I+LAKVDAT E++LA +F ++GYPT+KFFK G+ +Y GR A+ I+SWL+K+TGP A TL V +A+++I +V V+GFFKD S +KAF A++ DD+ FG+TS +VF +F+V+ D S+VLFKKFDEGRN F G + + FI QLP+VIEF ++TA KIFGG IK H+L F+ K ++ + + QF+ +A+ F G++LF+ I++D +DN R+LEFFG+ K + P R+I LE++M K+KP+ + I++EN+ F S ++G +KPH+M+Q+IPEDWDKNPVKVLVGKNF+ VA + + VFVEFYAPWCGHCKQLAPIWDQLGE +KDN +IV+AKMDSTANE+E V++ SFPT+K+FP D +++D+ G+RTL+ F KF++S
Sbjct: 23 DVLVLKKSNFEE-ALKAHPNVLVEFYAPWCGHCKALAPEYSKAAGMLKAEGSDIRLAKVDATEESELAQEFGVRGYPTIKFFKGGEKGNPKEYSAGRQAEDIVSWLKKRTGPAATTLNDVMQAESIIADNEVAVIGFFKDVESEDSKAFIKTAEAVDDIPFGITSDDSVFAKFEVAKD-SVVLFKKFDEGRNTFDGEVSKESLLNFIKANQLPLVIEFTEQTAPKIFGGDIKSHILMFVPKAAKDFQDKMDQFKKAAEGFKGKILFIFIDSDVDDNQRILEFFGLKKEECPVIRLITLEEEMTKYKPESSEITAENIISFCTSFVEGTLKPHLMSQDIPEDWDKNPVKVLVGKNFEEVAFNPANNVFVEFYAPWCGHCKQLAPIWDQLGEKFKDNANIVVAKMDSTANEIEAVKVHSFPTLKFFPAGDERKVIDYNGERTLDGFTKFLES 470 HSP 2 Score: 117.857 bits (294), Expect = 2.353e-27 Identity = 59/124 (47.58%), Postives = 79/124 (63.71%), Query Frame = 3
Query: 120 LSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIVN 479
L ++ C A A+I EEE VLVL SNF E ++ + VLVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q +G++G+PTIK F G K +PK+Y R A IV+
Sbjct: 2 LKFLIVCALAAMSAAEIAEEEDVLVLKKSNFEEALKAHPNVLVEFYAPWCGHCKALAPEYSKAAGMLKAEGSDIRLAKVDATEESELAQEFGVRGYPTIKFFKGGEKGNPKEYSAGRQAEDIVS 125 HSP 3 Score: 70.4774 bits (171), Expect = 3.468e-12 Identity = 43/125 (34.40%), Postives = 58/125 (46.40%), Query Frame = 3
Query: 123 SIVLFCFALAQ-------CNADITEE---EGVLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERT 461
+I+ FC + + + DI E+ V VL NF EV V VEFYAPWCGHC+ LAP W + K + MD ++ + + FPT+K F + DY GERT
Sbjct: 336 NIISFCTSFVEGTLKPHLMSQDIPEDWDKNPVKVLVGKNFEEVAFNPANNVFVEFYAPWCGHCKQLAPIWDQLGEKFKDNANIVVAKMDSTANEIEAVKVHSFPTLKFFPAGDERKVIDYNGERT 460
BLAST of Protein disulfide-isomerase vs. Ensembl Zebrafish
Match: p4hb (prolyl 4-hydroxylase, beta polypeptide [Source:ZFIN;Acc:ZDB-GENE-080610-1]) HSP 1 Score: 501.516 bits (1290), Expect = 2.466e-170 Identity = 246/450 (54.67%), Postives = 338/450 (75.11%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK---VMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPK-DSDEMVDFEGDRTLETFKKFIDS 1928
DV+ L SNF+E LK+ LVEF+APWCGHCKALAPEY+ AA LK + S I+LAKVDAT E++LA +F ++GYPT+KFFK G+ +Y GR A+ I+SWL+K+TGP A TL V +A+++I +V V+GFFKD S +KAF A++ DD+ FG+TS +VF +F+V+ D S+VLFKKFDEGRN F G + + FI QLP+VIEF ++TA KIFGG IK H+L F+ K ++ + + QF+ +A+ F G++LF+ I++D +DN R+LEFFG+ K + P R+I LE++M K+KP+ + I++EN+ F S ++G +KPH+M+Q+IPEDWDKNPVKVLVGKNF+ VA + + VFVEFYAPWCGHCKQLAPIWDQLGE +KDN +IV+AKMDSTANE+E V++ SFPT+K+FP D +++D+ G+RTL+ F KF++S
Sbjct: 23 DVLVLKKSNFEE-ALKAHPNVLVEFYAPWCGHCKALAPEYSKAAGMLKAEGSDIRLAKVDATEESELAQEFGVRGYPTIKFFKGGEKGNPKEYSAGRQAEDIVSWLKKRTGPAATTLNDVMQAESIIADNEVAVIGFFKDVESEDSKAFIKTAEAVDDIPFGITSDDSVFAKFEVAKD-SVVLFKKFDEGRNTFDGEVSKESLLNFIKANQLPLVIEFTEQTAPKIFGGDIKSHILMFVPKAAKDFQDKMDQFKKAAEGFKGKILFIFIDSDVDDNQRILEFFGLKKEECPVIRLITLEEEMTKYKPESSEITAENIISFCTSFVEGTLKPHLMSQDIPEDWDKNPVKVLVGKNFEEVAFNPANNVFVEFYAPWCGHCKQLAPIWDQLGEKFKDNANIVVAKMDSTANEIEAVKVHSFPTLKFFPAGDERKVIDYNGERTLDGFTKFLES 470 HSP 2 Score: 117.857 bits (294), Expect = 2.353e-27 Identity = 59/124 (47.58%), Postives = 79/124 (63.71%), Query Frame = 3
Query: 120 LSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIVN 479
L ++ C A A+I EEE VLVL SNF E ++ + VLVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q +G++G+PTIK F G K +PK+Y R A IV+
Sbjct: 2 LKFLIVCALAAMSAAEIAEEEDVLVLKKSNFEEALKAHPNVLVEFYAPWCGHCKALAPEYSKAAGMLKAEGSDIRLAKVDATEESELAQEFGVRGYPTIKFFKGGEKGNPKEYSAGRQAEDIVS 125 HSP 3 Score: 70.4774 bits (171), Expect = 3.468e-12 Identity = 43/125 (34.40%), Postives = 58/125 (46.40%), Query Frame = 3
Query: 123 SIVLFCFALAQ-------CNADITEE---EGVLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERT 461
+I+ FC + + + DI E+ V VL NF EV V VEFYAPWCGHC+ LAP W + K + MD ++ + + FPT+K F + DY GERT
Sbjct: 336 NIISFCTSFVEGTLKPHLMSQDIPEDWDKNPVKVLVGKNFEEVAFNPANNVFVEFYAPWCGHCKQLAPIWDQLGEKFKDNANIVVAKMDSTANEIEAVKVHSFPTLKFFPAGDERKVIDYNGERT 460
BLAST of Protein disulfide-isomerase vs. Ensembl Zebrafish
Match: pdia2 (protein disulfide isomerase family A, member 2 [Source:ZFIN;Acc:ZDB-GENE-040426-705]) HSP 1 Score: 416.001 bits (1068), Expect = 1.631e-136 Identity = 219/456 (48.03%), Postives = 305/456 (66.89%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMD---YGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK------DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSD-EMVDFEGDRTLETFKKFIDS 1928
DV+ L NFD L + LVEF+APWCGHC++L P YA A +LK +S ++LAKVDA E +LAS+F + +PTLKFFK+G + + G RT I WLEK T P A L V+ A+AL+E+ +V+VVGFFKD AK F V DV FG+TS +F +++V +D S+VLFKKFDE R D P D+ ++ +FI + +V+ FN+E A +IF ++KH+L F++ + L +F A +F +V+F+ ++ E + VL++F +++ D P+ R+I ED +V + D +TI+ + L F Q V DG VKP++ +QEIPEDWDKNPVKVLVGKNF+ VA D+SK VFVEFYAPWCGHC+QLAP+WD+LGE YKD +I+IAKMD+T N+VE + IQ FPTIKYFP ++ ++VD++G+R LETF KF+D+
Sbjct: 57 DVLILHSVNFDR-ALSENKYLLVEFYAPWCGHCRSLEPIYAEVAGQLKNASSEVRLAKVDAIEEKELASEFSVDSFPTLKFFKEGNRQNATTFFGKRTLKGIKRWLEKHTAPSATVLNDVKSAEALLEANEVLVVGFFKDLEGEKAKTFYDVTLIAVDVNFGITSDPELFKKYEVKTD-SLVLFKKFDERRADM--PLSDETKLDKGEMISFIHSNSMRLVVPFNEENAEQIFNSKVRKHLLLFLNTTVDSQNALVEEFREVASEFKEKVIFITVDVTAEKVNHVLKYFSISEDDVPTIRLINTED-VVTYAMDGSTINKDTLRTFCQGVFDGTVKPYLKSQEIPEDWDKNPVKVLVGKNFNEVAFDESKNVFVEFYAPWCGHCQQLAPVWDELGEKYKDQENIIIAKMDATENDVEDLTIQGFPTIKYFPAGTEKKIVDYDGNRDLETFSKFLDN 507 HSP 2 Score: 88.5817 bits (218), Expect = 8.426e-18 Identity = 42/107 (39.25%), Postives = 66/107 (61.68%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIFGK-SKHSPKDYQGERTAGSI 473
+ITE++ VL+L + NF+ + +NKY+LVEFYAPWCGHC+SL P + + A LK V++ VD + L + + FPT+K F + ++ + + G+RT I
Sbjct: 51 EITEDKDVLILHSVNFDRALSENKYLLVEFYAPWCGHCRSLEPIYAEVAGQLKNASSEVRLAKVDAIEEKELASEFSVDSFPTLKFFKEGNRQNATTFFGKRTLKGI 157 HSP 3 Score: 77.7962 bits (190), Expect = 2.176e-14 Identity = 42/93 (45.16%), Postives = 51/93 (54.84%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGER 458
V VL NFNEV +++K V VEFYAPWCGHCQ LAP W + K + MD + + IQGFPTIK F ++ DY G R
Sbjct: 404 VKVLVGKNFNEVAFDESKNVFVEFYAPWCGHCQQLAPVWDELGEKYKDQENIIIAKMDATENDVEDLTIQGFPTIKYFPAGTEKKIVDYDGNR 496
BLAST of Protein disulfide-isomerase vs. Ensembl Zebrafish
Match: pdia2 (protein disulfide isomerase family A, member 2 [Source:ZFIN;Acc:ZDB-GENE-040426-705]) HSP 1 Score: 416.387 bits (1069), Expect = 1.660e-136 Identity = 219/456 (48.03%), Postives = 305/456 (66.89%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMD---YGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK------DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSD-EMVDFEGDRTLETFKKFIDS 1928
DV+ L NFD L + LVEF+APWCGHC++L P YA A +LK +S ++LAKVDA E +LAS+F + +PTLKFFK+G + + G RT I WLEK T P A L V+ A+AL+E+ +V+VVGFFKD AK F V DV FG+TS +F +++V +D S+VLFKKFDE R D P D+ ++ +FI + +V+ FN+E A +IF ++KH+L F++ + L +F A +F +V+F+ ++ E + VL++F +++ D P+ R+I ED +V + D +TI+ + L F Q V DG VKP++ +QEIPEDWDKNPVKVLVGKNF+ VA D+SK VFVEFYAPWCGHC+QLAP+WD+LGE YKD +I+IAKMD+T N+VE + IQ FPTIKYFP ++ ++VD++G+R LETF KF+D+
Sbjct: 57 DVLILHSVNFDR-ALSENKYLLVEFYAPWCGHCRSLEPIYAEVAGQLKNASSEVRLAKVDAIEEKELASEFSVDSFPTLKFFKEGNRQNATTFFGKRTLKGIKRWLEKHTAPSATVLNDVKSAEALLEANEVLVVGFFKDLEGEKAKTFYDVTLIAVDVNFGITSDPELFKKYEVKTD-SLVLFKKFDERRADM--PLSDETKLDKGEMISFIHSNSMRLVVPFNEENAEQIFNSKVRKHLLLFLNTTVDSQNALVEEFREVASEFKEKVIFITVDVTAEKVNHVLKYFSISEDDVPTIRLINTED-VVTYAMDGSTINKDTLRTFCQGVFDGTVKPYLKSQEIPEDWDKNPVKVLVGKNFNEVAFDESKNVFVEFYAPWCGHCQQLAPVWDELGEKYKDQENIIIAKMDATENDVEDLTIQGFPTIKYFPAGTEKKIVDYDGNRDLETFSKFLDN 507 HSP 2 Score: 88.9669 bits (219), Expect = 6.946e-18 Identity = 42/107 (39.25%), Postives = 66/107 (61.68%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIFGK-SKHSPKDYQGERTAGSI 473
+ITE++ VL+L + NF+ + +NKY+LVEFYAPWCGHC+SL P + + A LK V++ VD + L + + FPT+K F + ++ + + G+RT I
Sbjct: 51 EITEDKDVLILHSVNFDRALSENKYLLVEFYAPWCGHCRSLEPIYAEVAGQLKNASSEVRLAKVDAIEEKELASEFSVDSFPTLKFFKEGNRQNATTFFGKRTLKGI 157 HSP 3 Score: 78.1814 bits (191), Expect = 1.515e-14 Identity = 42/93 (45.16%), Postives = 51/93 (54.84%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGER 458
V VL NFNEV +++K V VEFYAPWCGHCQ LAP W + K + MD + + IQGFPTIK F ++ DY G R
Sbjct: 404 VKVLVGKNFNEVAFDESKNVFVEFYAPWCGHCQQLAPVWDELGEKYKDQENIIIAKMDATENDVEDLTIQGFPTIKYFPAGTEKKIVDYDGNR 496
BLAST of Protein disulfide-isomerase vs. Ensembl Zebrafish
Match: pdia4 (protein disulfide isomerase family A, member 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5493]) HSP 1 Score: 319.701 bits (818), Expect = 2.344e-98 Identity = 218/609 (35.80%), Postives = 326/609 (53.53%), Query Frame = 3
Query: 168 ITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALI-ESKDVVVVGFF-KDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLF-KKFDEGRNDFTGPFVDKD------IRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETS-----AKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEV--EGVRIQSFPTIKYFPKDSDEM-VDFE-GDRTLETFKKFIDSN 1931
+ EE GVLVLT++NF+ IE VLVEFYAPWCGHC+ APE++K A LK + V VD K S LG + + G+PTIKI K + P DY G+R+ +IV + + E+ + + LT NFD+ V+ + + LVEF+APWCGHCK LAPEY AA++L + I LAKVDAT E+DLA++F + GYPTLK F+ GK DY G R I+ ++ + GPP+ ++++++ + L+ + D V+VG F D+ +A E+ ++D F T V K S ++L +KF + KD ++ F K+ LP+V Q K + + + V+ + + QF S AK F + F I DEED L+ G++++ I E K+ + S+ L FV + GK+KP + +Q +P++ +K PVKV+VGK FD + D K V +EFYAPWCGHCK+L P + LG+ YK+ ++VIAKMD+TAN+V + +++ FPTI + P ++ + + FE G R +E F KF++ +
Sbjct: 55 VKEENGVLVLTDANFDTFIEGKDTVLVEFYAPWCGHCKQFAPEYEKIAQTLKENDPPIPVAKVDATKASGLGSRFEVSGYPTIKILKKGE--PLDYDGDRSEHAIV----ERVKEVAQPDWKPPPEA------------------TLVLTKDNFDD-VVNNADIILVEFYAPWCGHCKRLAPEYEKAAKELSNRTPPIPLAKVDATAESDLATRFGVSGYPTLKIFRKGKAFDYNGPREKFGIVDYMSDQAGPPSKQVQTLKQVQELLRDGDDAVIVGVFSSDEDAAYEIYQEACNSLREDYKFMHTFNNDVTKFLKASPGQVVMLQPEKFRSKYESASHSLTIKDSTPASEVQDFFKKHILPLVGHRKQSNDAKRY--TKRPLVVVYYGVDFSFDYRVATQFWRSKVLEVAKDF-PEYTFAI--ADEEDYADELKSLGLSESGEEVNVGIVGEGGK-KYAMEPEEFDSDVLRSFVMAFKKGKLKPIVKSQPLPKN-NKGPVKVVVGKTFDEIVMDSKKDVLIEFYAPWCGHCKKLEPDYISLGKKYKNEKNLVIAKMDATANDVPHDSYKVEGFPTIYFAPSNNKQNPIKFEGGKRDVEEFSKFVEKH 631
BLAST of Protein disulfide-isomerase vs. Ensembl Xenopus
Match: p4hb (prolyl 4-hydroxylase subunit beta [Source:NCBI gene;Acc:395048]) HSP 1 Score: 483.026 bits (1242), Expect = 4.476e-163 Identity = 242/451 (53.66%), Postives = 324/451 (71.84%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMV-DFEGDRTLETFKKFIDSN 1931
DV+ L NFDE LK LVEF+APWCGHCKALAPEY AA LK + I+L KVDAT E+DLA +F ++GYPT+KFFK+G +Y GR A I++WL+K+TGP A+TL AL++S +V V+GFFKD AS AK F A++ DD+ FG+TS +A F ++++ D IVLFKKFDEGRN + G +++ +FI +LP+VIEF ++TA IFGG IK H+L F+ K + Y E F+ +A F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ A +S+E + F S L+GKVKPH+M+Q++ +DWDKNPVK+LVGKNF+ V ++ K VFVEFYAPWCGHCKQLAPIWDQLGE YKD+ +I+IAKMDSTANE+E V+I SFPT+K+FP + V D+ G+RTLE F KF++S
Sbjct: 22 DVLVLKKDNFDE-ALKQYPFILVEFYAPWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVNWLKKRTGPAASTLGDEAGVAALVDSSEVAVIGFFKDPASEPAKVFLQAAEAVDDIPFGITSSEAAFSKYELGKD-GIVLFKKFDEGRNAYEGDITKEEVLSFIKANRLPLVIEFTEQTAPMIFGGEIKTHILFFLPKSASDYKEKLEDFKKAAASFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESADLSAEAIKEFCDSFLEGKVKPHLMSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNVADYNGERTLEGFSKFLESG 470 HSP 2 Score: 117.857 bits (294), Expect = 3.110e-27 Identity = 57/122 (46.72%), Postives = 79/122 (64.75%), Query Frame = 3
Query: 126 IVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
++ C L A+I EE VLVL NF+E +++ ++LVEFYAPWCGHC++LAPE++KAA LK +++G VD + S L Q +G++G+PTIK F K SPK+Y R A IVN
Sbjct: 3 LLFGCSLLIVARANIPEERDVLVLKKDNFDEALKQYPFILVEFYAPWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVN 124 HSP 3 Score: 73.1738 bits (178), Expect = 7.139e-13 Identity = 42/108 (38.89%), Postives = 58/108 (53.70%), Query Frame = 3
Query: 147 LAQCNADITEEEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF--GKSKHSPKDYQGERT 461
++Q +D ++ V +L NF EV+ + K V VEFYAPWCGHC+ LAP W + K + MD ++ + I FPT+K F G K+ DY GERT
Sbjct: 353 MSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNV-ADYNGERT 459
BLAST of Protein disulfide-isomerase vs. Ensembl Xenopus
Match: p4hb (prolyl 4-hydroxylase subunit beta [Source:NCBI gene;Acc:395048]) HSP 1 Score: 483.797 bits (1244), Expect = 4.535e-163 Identity = 242/451 (53.66%), Postives = 324/451 (71.84%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMV-DFEGDRTLETFKKFIDSN 1931
DV+ L NFDE LK LVEF+APWCGHCKALAPEY AA LK + I+L KVDAT E+DLA +F ++GYPT+KFFK+G +Y GR A I++WL+K+TGP A+TL AL++S +V V+GFFKD AS AK F A++ DD+ FG+TS +A F ++++ D IVLFKKFDEGRN + G +++ +FI +LP+VIEF ++TA IFGG IK H+L F+ K + Y E F+ +A F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ A +S+E + F S L+GKVKPH+M+Q++ +DWDKNPVK+LVGKNF+ V ++ K VFVEFYAPWCGHCKQLAPIWDQLGE YKD+ +I+IAKMDSTANE+E V+I SFPT+K+FP + V D+ G+RTLE F KF++S
Sbjct: 42 DVLVLKKDNFDE-ALKQYPFILVEFYAPWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVNWLKKRTGPAASTLGDEAGVAALVDSSEVAVIGFFKDPASEPAKVFLQAAEAVDDIPFGITSSEAAFSKYELGKD-GIVLFKKFDEGRNAYEGDITKEEVLSFIKANRLPLVIEFTEQTAPMIFGGEIKTHILFFLPKSASDYKEKLEDFKKAAASFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESADLSAEAIKEFCDSFLEGKVKPHLMSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNVADYNGERTLEGFSKFLESG 490 HSP 2 Score: 120.168 bits (300), Expect = 6.870e-28 Identity = 61/134 (45.52%), Postives = 85/134 (63.43%), Query Frame = 3
Query: 96 VLF*KMKYLSIVLF--CFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
VL+ ++ + VL C L A+I EE VLVL NF+E +++ ++LVEFYAPWCGHC++LAPE++KAA LK +++G VD + S L Q +G++G+PTIK F K SPK+Y R A IVN
Sbjct: 11 VLYTRLIMMRAVLLFGCSLLIVARANIPEERDVLVLKKDNFDEALKQYPFILVEFYAPWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVN 144 HSP 3 Score: 73.1738 bits (178), Expect = 7.731e-13 Identity = 42/108 (38.89%), Postives = 58/108 (53.70%), Query Frame = 3
Query: 147 LAQCNADITEEEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF--GKSKHSPKDYQGERT 461
++Q +D ++ V +L NF EV+ + K V VEFYAPWCGHC+ LAP W + K + MD ++ + I FPT+K F G K+ DY GERT
Sbjct: 373 MSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNV-ADYNGERT 479
BLAST of Protein disulfide-isomerase vs. Ensembl Xenopus
Match: p4hb (prolyl 4-hydroxylase subunit beta [Source:NCBI gene;Acc:395048]) HSP 1 Score: 483.026 bits (1242), Expect = 4.629e-163 Identity = 242/451 (53.66%), Postives = 324/451 (71.84%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMV-DFEGDRTLETFKKFIDSN 1931
DV+ L NFDE LK LVEF+APWCGHCKALAPEY AA LK + I+L KVDAT E+DLA +F ++GYPT+KFFK+G +Y GR A I++WL+K+TGP A+TL AL++S +V V+GFFKD AS AK F A++ DD+ FG+TS +A F ++++ D IVLFKKFDEGRN + G +++ +FI +LP+VIEF ++TA IFGG IK H+L F+ K + Y E F+ +A F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ A +S+E + F S L+GKVKPH+M+Q++ +DWDKNPVK+LVGKNF+ V ++ K VFVEFYAPWCGHCKQLAPIWDQLGE YKD+ +I+IAKMDSTANE+E V+I SFPT+K+FP + V D+ G+RTLE F KF++S
Sbjct: 15 DVLVLKKDNFDE-ALKQYPFILVEFYAPWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVNWLKKRTGPAASTLGDEAGVAALVDSSEVAVIGFFKDPASEPAKVFLQAAEAVDDIPFGITSSEAAFSKYELGKD-GIVLFKKFDEGRNAYEGDITKEEVLSFIKANRLPLVIEFTEQTAPMIFGGEIKTHILFFLPKSASDYKEKLEDFKKAAASFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESADLSAEAIKEFCDSFLEGKVKPHLMSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNVADYNGERTLEGFSKFLESG 463 HSP 2 Score: 114.775 bits (286), Expect = 3.111e-26 Identity = 55/110 (50.00%), Postives = 75/110 (68.18%), Query Frame = 3
Query: 162 ADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
A+I EE VLVL NF+E +++ ++LVEFYAPWCGHC++LAPE++KAA LK +++G VD + S L Q +G++G+PTIK F K SPK+Y R A IVN
Sbjct: 8 ANIPEERDVLVLKKDNFDEALKQYPFILVEFYAPWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVN 117 HSP 3 Score: 73.559 bits (179), Expect = 6.243e-13 Identity = 42/108 (38.89%), Postives = 58/108 (53.70%), Query Frame = 3
Query: 147 LAQCNADITEEEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF--GKSKHSPKDYQGERT 461
++Q +D ++ V +L NF EV+ + K V VEFYAPWCGHC+ LAP W + K + MD ++ + I FPT+K F G K+ DY GERT
Sbjct: 346 MSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNV-ADYNGERT 452
BLAST of Protein disulfide-isomerase vs. Ensembl Xenopus
Match: p4hb (prolyl 4-hydroxylase subunit beta [Source:NCBI gene;Acc:395048]) HSP 1 Score: 468.774 bits (1205), Expect = 6.366e-158 Identity = 229/426 (53.76%), Postives = 310/426 (72.77%), Query Frame = 3
Query: 666 FAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMV-DFEGDRTLETFKKFIDSN 1931
+APWCGHCKALAPEY AA LK + I+L KVDAT E+DLA +F ++GYPT+KFFK+G +Y GR A I++WL+K+TGP A+TL AL++S +V V+GFFKD AS AK F A++ DD+ FG+TS +A F ++++ D IVLFKKFDEGRN + G +++ +FI +LP+VIEF ++TA IFGG IK H+L F+ K + Y E F+ +A F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ A +S+E + F S L+GKVKPH+M+Q++ +DWDKNPVK+LVGKNF+ V ++ K VFVEFYAPWCGHCKQLAPIWDQLGE YKD+ +I+IAKMDSTANE+E V+I SFPT+K+FP + V D+ G+RTLE F KF++S
Sbjct: 18 YAPWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVNWLKKRTGPAASTLGDEAGVAALVDSSEVAVIGFFKDPASEPAKVFLQAAEAVDDIPFGITSSEAAFSKYELGKD-GIVLFKKFDEGRNAYEGDITKEEVLSFIKANRLPLVIEFTEQTAPMIFGGEIKTHILFFLPKSASDYKEKLEDFKKAAASFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESADLSAEAIKEFCDSFLEGKVKPHLMSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNVADYNGERTLEGFSKFLESG 442 HSP 2 Score: 85.5001 bits (210), Expect = 8.393e-17 Identity = 40/84 (47.62%), Postives = 55/84 (65.48%), Query Frame = 3
Query: 240 VLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
+ + YAPWCGHC++LAPE++KAA LK +++G VD + S L Q +G++G+PTIK F K SPK+Y R A IVN
Sbjct: 13 AMAKGYAPWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVN 96 HSP 3 Score: 83.1889 bits (204), Expect = 4.723e-16 Identity = 51/121 (42.15%), Postives = 71/121 (58.68%), Query Frame = 3
Query: 603 LTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEK--KTGPPAATLKSVEEA 950
L NF+E+V E+ VEF+APWCGHCK LAP + EK K+ +II +AK+D+T N++ + KI +PTLKFF G V DY G RT + +LE + G L+ +E+A
Sbjct: 341 LVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENII-IAKMDSTA-NEIEA-VKIHSFPTLKFFPAGPGKNVADYNGERTLEGFSKFLESGGQDGAADEDLEDLEDA 458 HSP 4 Score: 72.7886 bits (177), Expect = 8.358e-13 Identity = 42/108 (38.89%), Postives = 58/108 (53.70%), Query Frame = 3
Query: 147 LAQCNADITEEEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF--GKSKHSPKDYQGERT 461
++Q +D ++ V +L NF EV+ + K V VEFYAPWCGHC+ LAP W + K + MD ++ + I FPT+K F G K+ DY GERT
Sbjct: 325 MSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNV-ADYNGERT 431
BLAST of Protein disulfide-isomerase vs. Ensembl Xenopus
Match: p4hb (prolyl 4-hydroxylase subunit beta [Source:NCBI gene;Acc:395048]) HSP 1 Score: 467.618 bits (1202), Expect = 1.403e-157 Identity = 229/425 (53.88%), Postives = 309/425 (72.71%), Query Frame = 3
Query: 669 APWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMV-DFEGDRTLETFKKFIDSN 1931
APWCGHCKALAPEY AA LK + I+L KVDAT E+DLA +F ++GYPT+KFFK+G +Y GR A I++WL+K+TGP A+TL AL++S +V V+GFFKD AS AK F A++ DD+ FG+TS +A F ++++ D IVLFKKFDEGRN + G +++ +FI +LP+VIEF ++TA IFGG IK H+L F+ K + Y E F+ +A F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ A +S+E + F S L+GKVKPH+M+Q++ +DWDKNPVK+LVGKNF+ V ++ K VFVEFYAPWCGHCKQLAPIWDQLGE YKD+ +I+IAKMDSTANE+E V+I SFPT+K+FP + V D+ G+RTLE F KF++S
Sbjct: 13 APWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVNWLKKRTGPAASTLGDEAGVAALVDSSEVAVIGFFKDPASEPAKVFLQAAEAVDDIPFGITSSEAAFSKYELGKD-GIVLFKKFDEGRNAYEGDITKEEVLSFIKANRLPLVIEFTEQTAPMIFGGEIKTHILFFLPKSASDYKEKLEDFKKAAASFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESADLSAEAIKEFCDSFLEGKVKPHLMSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNVADYNGERTLEGFSKFLESG 436 HSP 2 Score: 83.1889 bits (204), Expect = 4.667e-16 Identity = 51/121 (42.15%), Postives = 71/121 (58.68%), Query Frame = 3
Query: 603 LTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEK--KTGPPAATLKSVEEA 950
L NF+E+V E+ VEF+APWCGHCK LAP + EK K+ +II +AK+D+T N++ + KI +PTLKFF G V DY G RT + +LE + G L+ +E+A
Sbjct: 335 LVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENII-IAKMDSTA-NEIEA-VKIHSFPTLKFFPAGPGKNVADYNGERTLEGFSKFLESGGQDGAADEDLEDLEDA 452 HSP 3 Score: 82.4185 bits (202), Expect = 7.268e-16 Identity = 39/78 (50.00%), Postives = 52/78 (66.67%), Query Frame = 3
Query: 258 APWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
APWCGHC++LAPE++KAA LK +++G VD + S L Q +G++G+PTIK F K SPK+Y R A IVN
Sbjct: 13 APWCGHCKALAPEYEKAAGVLKSEGLPIRLGKVDATEESDLAQEFGVRGYPTIKFFKNGDKASPKEYSAGREAADIVN 90 HSP 4 Score: 72.7886 bits (177), Expect = 8.629e-13 Identity = 42/108 (38.89%), Postives = 58/108 (53.70%), Query Frame = 3
Query: 147 LAQCNADITEEEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF--GKSKHSPKDYQGERT 461
++Q +D ++ V +L NF EV+ + K V VEFYAPWCGHC+ LAP W + K + MD ++ + I FPT+K F G K+ DY GERT
Sbjct: 319 MSQDVSDDWDKNPVKILVGKNFEEVVFNEEKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHENIIIAKMDSTANEIEAVKIHSFPTLKFFPAGPGKNV-ADYNGERT 425
BLAST of Protein disulfide-isomerase vs. Ensembl Mouse
Match: P4hb (prolyl 4-hydroxylase, beta polypeptide [Source:MGI Symbol;Acc:MGI:97464]) HSP 1 Score: 491.115 bits (1263), Expect = 2.880e-166 Identity = 244/450 (54.22%), Postives = 332/450 (73.78%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDE-MVDFEGDRTLETFKKFIDS 1928
+V+ L SNF+E L + + LVEF+APWCGHCKALAPEYA AA KLK + S I+LAKVDAT E+DLA ++ ++GYPT+KFFK+G +Y GR AD I++WL+K+TGP A TL A++L++S +V V+GFFKD S +AK F A++ DD+ FG+TS VF ++++ D +VLFKKFDEGRN+F G + + FI QLP+VIEF ++TA KIFGG IK H+L F+ K Y F+ +A+ F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ +++E + F L+GK+KPH+M+QE+PEDWDK PVKVLVG NF+ VA D+ K VFVEFYAPWCGHCKQLAPIWD+LGETYKD+ +I+IAKMDSTANEVE V++ SFPT+K+FP +D ++D+ G+RTL+ FKKF++S
Sbjct: 27 NVLVLKKSNFEE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDVESDSAKQFLLAAEAIDDIPFGITSNSGVFSKYQLDKD-GVVLFKKFDEGRNNFEGEITKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSSFKRAAEGFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLES 474 HSP 2 Score: 103.99 bits (258), Expect = 9.158e-23 Identity = 55/108 (50.93%), Postives = 71/108 (65.74%), Query Frame = 3
Query: 168 ITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
+ EE+ VLVL SNF E + +KY+LVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q YG++G+PTIK F SPK+Y R A IVN
Sbjct: 22 LEEEDNVLVLKKSNFEEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVN 129 HSP 3 Score: 75.8702 bits (185), Expect = 8.048e-14 Identity = 40/94 (42.55%), Postives = 51/94 (54.26%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSK-HSPKDYQGERT 461
V VL +NF EV ++ K V VEFYAPWCGHC+ LAP W K K + MD ++ + + FPT+K F S + DY GERT
Sbjct: 371 VKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERT 464
BLAST of Protein disulfide-isomerase vs. Ensembl Mouse
Match: Pdia2 (protein disulfide isomerase associated 2 [Source:MGI Symbol;Acc:MGI:1916441]) HSP 1 Score: 378.252 bits (970), Expect = 4.326e-122 Identity = 193/447 (43.18%), Postives = 287/447 (64.21%), Query Frame = 3
Query: 627 MVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMD---YGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK-------DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDA-TISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSD-EMVDFEGDRTLETFKKFIDSN 1931
+ L+ +VEF+APWCGHCK LAPEY+ AA L +++++ LAKVD E +L +F++ GYPTLKFF++G + Y G +TA+ I WL ++ GP A L+ E +AL+ D+VV+GFF+D F ++A D+ FG T Q +F++F ++ D ++VLFKKFDEGR DF VDK D+ F+ + + +V EFN +T+ KIF I H+L F+++ ++ EL F +A F GQVLFV+++ DN VL +FG+ + P+ R+I +E K+ P I++ ++ F Q+VL G++K ++++QEIP DWD+ PVK LV KNF+ VA D++K VFV+FYAPWC HCK++AP W+ L E YKD DIVIA++D+TANE+E + +PT+K+FP D +++D++ R LETF KF+DS
Sbjct: 57 LALQEHSALMVEFYAPWCGHCKELAPEYSKAAALLAAESAVVTLAKVDGPAEPELTKEFEVVGYPTLKFFQNGNRTNPEEYAGPKTAEGIAEWLRRRVGPSATHLEDEEGVQALMAKWDMVVIGFFQDLQGKDMATFLALAKDALDMTFGFTDQPQLFEKFGLTKD-TVVLFKKFDEGRADFP---VDKETGLDLGDLSRFLVIHSMHLVTEFNSQTSPKIFAAKILNHLLLFVNQTLAQHRELLTDFREAAPPFRGQVLFVMVDV-AADNSHVLNYFGLKAEEAPTLRLINVETTK-KYAPTGVIAITAASVAAFCQAVLHGEIKHYLLSQEIPPDWDQGPVKTLVSKNFEQVAFDETKNVFVKFYAPWCSHCKEMAPAWEALAEKYKDREDIVIAELDATANELEAFSVLGYPTLKFFPAGPDRKVIDYKSTRDLETFSKFLDSG 497 HSP 2 Score: 81.2629 bits (199), Expect = 1.978e-15 Identity = 40/108 (37.04%), Postives = 67/108 (62.04%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL---KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIV 476
++ +E+G+LVL + + ++++ ++VEFYAPWCGHC+ LAPE+ KAA L VV + VD L + + + G+PT+K F ++ +P++Y G +TA I
Sbjct: 40 EVPKEDGILVLNHRTLSLALQEHSALMVEFYAPWCGHCKELAPEYSKAAALLAAESAVVTLAKVDGPAEPELTKEFEVVGYPTLKFFQNGNRTNPEEYAGPKTAEGIA 147 HSP 3 Score: 78.5666 bits (192), Expect = 1.343e-14 Identity = 43/106 (40.57%), Postives = 65/106 (61.32%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLE 902
V L NF+++ + V+F+APWC HCK +AP + + AEK K++ I+ +A++DAT N+L + F + GYPTLKFF G KV+DY R +T +L+
Sbjct: 393 VKTLVSKNFEQVAFDETKNVFVKFYAPWCSHCKEMAPAWEALAEKYKDREDIV-IAELDATA-NELEA-FSVLGYPTLKFFPAGPDRKVIDYKSTRDLETFSKFLD 495 HSP 4 Score: 61.2326 bits (147), Expect = 3.155e-9 Identity = 27/78 (34.62%), Postives = 45/78 (57.69%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF 416
V L + NF +V ++ K V V+FYAPWC HC+ +AP W+ A K + ++D ++ + + + G+PT+K F
Sbjct: 393 VKTLVSKNFEQVAFDETKNVFVKFYAPWCSHCKEMAPAWEALAEKYKDREDIVIAELDATANELEAFSVLGYPTLKFF 470 HSP 5 Score: 57.3806 bits (137), Expect = 5.515e-8 Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 3
Query: 1650 SVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIV-IAKMDSTANE--VEGVRIQSFPTIKYF 1853
S+A + A+ VEFYAPWCGHCK+LAP + + + +V +AK+D A + + +PT+K+F
Sbjct: 56 SLALQEHSALMVEFYAPWCGHCKELAPEYSKAAALLAAESAVVTLAKVDGPAEPELTKEFEVVGYPTLKFF 126
BLAST of Protein disulfide-isomerase vs. Ensembl Mouse
Match: Pdia2 (protein disulfide isomerase associated 2 [Source:MGI Symbol;Acc:MGI:1916441]) HSP 1 Score: 373.244 bits (957), Expect = 2.790e-120 Identity = 193/447 (43.18%), Postives = 286/447 (63.98%), Query Frame = 3
Query: 627 MVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMD---YGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK-------DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDA-TISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSD-EMVDFEGDRTLETFKKFIDSN 1931
+ L+ +VEF+APWCGHCK LAPEY+ AA L +++++ LAKVD E +L +F++ GYPTLKFF++G + Y G +TA+ I WL ++ GP A L+ E +AL+ D+VV+GFF+ + A A A D+ FG T Q +F++F ++ D ++VLFKKFDEGR DF VDK D+ F+ + + +V EFN +T+ KIF I H+L F+++ ++ EL F +A F GQVLFV+++ DN VL +FG+ + P+ R+I +E K+ P I++ ++ F Q+VL G++K ++++QEIP DWD+ PVK LV KNF+ VA D++K VFV+FYAPWC HCK++AP W+ L E YKD DIVIA++D+TANE+E + +PT+K+FP D +++D++ R LETF KF+DS
Sbjct: 57 LALQEHSALMVEFYAPWCGHCKELAPEYSKAAALLAAESAVVTLAKVDGPAEPELTKEFEVVGYPTLKFFQNGNRTNPEEYAGPKTAEGIAEWLRRRVGPSATHLEDEEGVQALMAKWDMVVIGFFQGKDMATFLALAKDAL---DMTFGFTDQPQLFEKFGLTKD-TVVLFKKFDEGRADFP---VDKETGLDLGDLSRFLVIHSMHLVTEFNSQTSPKIFAAKILNHLLLFVNQTLAQHRELLTDFREAAPPFRGQVLFVMVDV-AADNSHVLNYFGLKAEEAPTLRLINVETTK-KYAPTGVIAITAASVAAFCQAVLHGEIKHYLLSQEIPPDWDQGPVKTLVSKNFEQVAFDETKNVFVKFYAPWCSHCKEMAPAWEALAEKYKDREDIVIAELDATANELEAFSVLGYPTLKFFPAGPDRKVIDYKSTRDLETFSKFLDSG 494 HSP 2 Score: 80.8777 bits (198), Expect = 2.029e-15 Identity = 40/108 (37.04%), Postives = 67/108 (62.04%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL---KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIV 476
++ +E+G+LVL + + ++++ ++VEFYAPWCGHC+ LAPE+ KAA L VV + VD L + + + G+PT+K F ++ +P++Y G +TA I
Sbjct: 40 EVPKEDGILVLNHRTLSLALQEHSALMVEFYAPWCGHCKELAPEYSKAAALLAAESAVVTLAKVDGPAEPELTKEFEVVGYPTLKFFQNGNRTNPEEYAGPKTAEGIA 147 HSP 3 Score: 78.5666 bits (192), Expect = 1.403e-14 Identity = 43/106 (40.57%), Postives = 65/106 (61.32%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLE 902
V L NF+++ + V+F+APWC HCK +AP + + AEK K++ I+ +A++DAT N+L + F + GYPTLKFF G KV+DY R +T +L+
Sbjct: 390 VKTLVSKNFEQVAFDETKNVFVKFYAPWCSHCKEMAPAWEALAEKYKDREDIV-IAELDATA-NELEA-FSVLGYPTLKFFPAGPDRKVIDYKSTRDLETFSKFLD 492 HSP 4 Score: 61.2326 bits (147), Expect = 3.394e-9 Identity = 27/78 (34.62%), Postives = 45/78 (57.69%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF 416
V L + NF +V ++ K V V+FYAPWC HC+ +AP W+ A K + ++D ++ + + + G+PT+K F
Sbjct: 390 VKTLVSKNFEQVAFDETKNVFVKFYAPWCSHCKEMAPAWEALAEKYKDREDIVIAELDATANELEAFSVLGYPTLKFF 467 HSP 5 Score: 57.3806 bits (137), Expect = 5.539e-8 Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 3
Query: 1650 SVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIV-IAKMDSTANE--VEGVRIQSFPTIKYF 1853
S+A + A+ VEFYAPWCGHCK+LAP + + + +V +AK+D A + + +PT+K+F
Sbjct: 56 SLALQEHSALMVEFYAPWCGHCKELAPEYSKAAALLAAESAVVTLAKVDGPAEPELTKEFEVVGYPTLKFF 126
BLAST of Protein disulfide-isomerase vs. Ensembl Mouse
Match: Pdia4 (protein disulfide isomerase associated 4 [Source:MGI Symbol;Acc:MGI:104864]) HSP 1 Score: 309.686 bits (792), Expect = 1.446e-94 Identity = 211/609 (34.65%), Postives = 320/609 (52.55%), Query Frame = 3
Query: 183 GVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAA---TLKSVEEAKALIESKDVVVVGFFK-DQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVL----FKKFDEGR---NDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVL--------FVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEV--EGVRIQSFPTIKYFPK-DSDEMVDFE-GDRTLETFKKFIDSN 1931
GV VL + NF+ + VL+EFYAPWCGHC+ APE++K A+ LK + V +D S L + + G+PTIKI K + DY G RT IV A ++E+ + + + LT NFD+ V+ + + LVEF+APWCGHCK LAPEY AA++L +++ I LAKVDAT + DLA +F + GYPTLK F+ G+ DY G R I+ ++ +++GPP+ TLK V+E L + DVV++G F+ D A + ++ + ++D F T + KVS ++ F+ E R D G I+ ++ K+ LP+V K + S + V+ + S + + + + + +VL + DEED ++ G++++ I L++ KF + S+ L FV + GK+KP + +Q +P++ +K PVKV+VGK FD++ D K V +EFYAPWCGHCKQL PI+ LG+ YK D+VIAKMD+TAN++ + +++ FPTI + P D + FE G+R LE KFID +
Sbjct: 59 GVWVLNDGNFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASKFDVSGYPTIKILKKGQAV--DYDGSRTQEEIV-AKVREVSQPDWTPPPEV---------------------TLSLTKDNFDD-VVNNADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFRKGRPFDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQE--FLKDGDDVVIIGLFQGDGDPAYLQYQDAANNLREDYKFHHTFSPEIAKFLKVSLGKLVLTHPEKFQSKYEPRFHVMDVQGSTEASAIKDYVVKHALPLVGHRKTSNDAKRY--SKRPLVVVYYS------VDFSFDYRAATQFWRNKVLEVAKDFPEYTFAIADEEDYATEVKDLGLSESGEDVNAAI-LDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKN-NKGPVKVVVGKTFDAIVMDPKKDVLIEFYAPWCGHCKQLEPIYTSLGKKYKGQKDLVIAKMDATANDITNDQYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKFIDEH 630 HSP 2 Score: 89.7373 bits (221), Expect = 5.367e-18 Identity = 44/108 (40.74%), Postives = 65/108 (60.19%), Query Frame = 3
Query: 180 EGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL---KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKE 494
E L LT NF++V+ +LVEFYAPWCGHC+ LAPE++KAA L + + VD + + L + + + G+PT+KIF K + P DY G R IV+ +++
Sbjct: 173 EVTLSLTKDNFDDVVNNADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFRKGR--PFDYNGPREKYGIVDYMIEQ 278 HSP 3 Score: 79.7221 bits (195), Expect = 6.619e-15 Identity = 44/100 (44.00%), Postives = 61/100 (61.00%), Query Frame = 3
Query: 1614 NPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDN-TDIVIAKMDSTANEVEGVR--IQSFPTIKYFPKDSDEMVDFEGDRTLE 1904
N V VL NFD+ DK V +EFYAPWCGHCKQ AP ++++ T KDN I +AK+D+T+ + + + +PTIK K + VD++G RT E
Sbjct: 58 NGVWVLNDGNFDNFVADKD-TVLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASKFDVSGYPTIKILKK--GQAVDYDGSRTQE 154
BLAST of Protein disulfide-isomerase vs. Ensembl Mouse
Match: Pdia6 (protein disulfide isomerase associated 6 [Source:MGI Symbol;Acc:MGI:1919103]) HSP 1 Score: 266.929 bits (681), Expect = 9.420e-81 Identity = 152/309 (49.19%), Postives = 194/309 (62.78%), Query Frame = 3
Query: 111 MKYLSIVLFCFALAQC------NADITEEEGVLVLTNSNFN-EVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKN-SIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKV-MDYGGGRTADTIISW---LEKKTGPPAATLKSVEE--AKALIESKDVVVVGFF 995
M+ + + L C + + + V+ LT SNFN EVI+ + LVEFYAPWCGHCQ L PEWKKAATALK VVKVGAV+ DK+ SLG YG+QGFPTIKIFG +K+ P+DYQG RT +IV+AAL L +LV+ RL K G S S DVVELTD FD+ VL SE+ W+VEF+APWCGHCK L PE+A+AA ++KE+ +KLA VDAT+ LAS++ I+G+PT+K F+ G+ +DY GGRT I+S L PP L+ + E AK E + VV
Sbjct: 1 MRVIGMARLVLGLVSCTFFLAVSGLYSSSDDVIELTPSNFNREVIQSDGLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVNADKHQSLGGQYGVQGFPTIKIFGANKNKPEDYQGGRTGEAIVDAALSALRQLVKDRL--GGRSGGYSSGKQGRGDSSSKKDVVELTDDTFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAATEVKEQTKGKVKLAAVDATMNQVLASRYGIKGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKKTCEEHQLCVVAVL 307 HSP 2 Score: 72.0182 bits (175), Expect = 1.020e-12 Identity = 42/100 (42.00%), Postives = 52/100 (52.00%), Query Frame = 3
Query: 1611 KNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNT--DIVIAKMDSTANEVEGVR--IQSFPTIKYFPKDSDEMVDFEGDRT 1898
K V L FD D VEFYAPWCGHCK L P W K+ T + +A +D+T N+V R I+ FPTIK F K + VD++G RT
Sbjct: 164 KKDVVELTDDTFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAATEVKEQTKGKVKLAAVDATMNQVLASRYGIKGFPTIKIFQK-GESPVDYDGGRT 262 HSP 3 Score: 66.2402 bits (160), Expect = 7.112e-11 Identity = 32/94 (34.04%), Postives = 44/94 (46.81%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETF 1910
L NF+ VEFYAPWCGHC++L P W + KD + D + +Q FPTIK F + ++ D++G RT E
Sbjct: 35 LTPSNFNREVIQSDGLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVNADKHQSLGGQYGVQGFPTIKIFGANKNKPEDYQGGRTGEAI 128
BLAST of Protein disulfide-isomerase vs. UniProt/SwissProt
Match: sp|Q17770|PDI2_CAEEL (Protein disulfide-isomerase 2 OS=Caenorhabditis elegans OX=6239 GN=pdi-2 PE=1 SV=1) HSP 1 Score: 517.694 bits (1332), Expect = 5.402e-176 Identity = 257/447 (57.49%), Postives = 337/447 (75.39%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
+V+ LT NFDE V+ E LVEF+APWCGHCK+LAPEYA AA +LKE+ S IKL K+DATV +++SKF+++GYPTLK F++GK +Y GGR D+II+WL+KKTGP A L + K L ES DVVV+G+FKD S AK F VA DDV FG++++ AV E ++ + IVLFKKFD+GR F ++T+I +L +V EF QETA IFGG IK H L F+SK+S ++A+L+ +F+ +AK+F G+VLFV INTD E+N R++EFFG+ K + P+ R+I LE+DM KFKPD I++EN++ F Q+ LDG VKPH+M+++IPEDWDKNPVK+LVGKNF+ VARD +K V VEFYAPWCGHCKQLAP WD+LGE + D+ IVIAKMDST NEVE V+IQSFPTIK+FP S+++VD+ GDRT+E F KF+++N
Sbjct: 24 NVIVLTKDNFDE-VINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGYFKDTTSDDAKTFLEVAAGIDDVPFGISTEDAVKSEIELKGE-GIVLFKKFDDGRVAFDEKLTQDGLKTWIQANRLALVSEFTQETASVIFGGEIKSHNLLFVSKESSEFAKLEQEFKNAAKQFKGKVLFVYINTDVEENARIMEFFGLKKDELPAIRLISLEEDMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNPVKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETN 468 HSP 2 Score: 115.931 bits (289), Expect = 6.638e-26 Identity = 56/118 (47.46%), Postives = 77/118 (65.25%), Query Frame = 3
Query: 132 LFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
LF L A I EEE V+VLT NF+EVI N+++LVEFYAPWCGHC+SLAPE+ KAAT LK +K+G +D + + + ++G+PT+K+F K P++Y G R SI+
Sbjct: 7 LFFLVLGASAAVIEEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGK--PQEYNGGRDHDSII 122 HSP 3 Score: 77.411 bits (189), Expect = 1.667e-13 Identity = 40/93 (43.01%), Postives = 48/93 (51.61%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIEKN-KYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V +L NF +V N K VLVEFYAPWCGHC+ LAP W K + MD + + IQ FPTIK F + DY G+RT
Sbjct: 365 VKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRT 457
BLAST of Protein disulfide-isomerase vs. UniProt/SwissProt
Match: sp|P07237|PDIA1_HUMAN (Protein disulfide-isomerase OS=Homo sapiens OX=9606 GN=P4HB PE=1 SV=3) HSP 1 Score: 506.908 bits (1304), Expect = 1.644e-171 Identity = 247/449 (55.01%), Postives = 333/449 (74.16%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDE-MVDFEGDRTLETFKKFIDS 1928
V+ L SNF E L + + LVEF+APWCGHCKALAPEYA AA KLK + S I+LAKVDAT E+DLA ++ ++GYPT+KFF++G +Y GR AD I++WL+K+TGP A TL A++L+ES +V V+GFFKD S +AK F A++ DD+ FG+TS VF ++++ D +VLFKKFDEGRN+F G +++ FI QLP+VIEF ++TA KIFGG IK H+L F+ K Y F+T+A+ F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ +++E + F L+GK+KPH+M+QE+PEDWDK PVKVLVGKNF+ VA D+ K VFVEFYAPWCGHCKQLAPIWD+LGETYKD+ +IVIAKMDSTANEVE V++ SFPT+K+FP +D ++D+ G+RTL+ FKKF++S
Sbjct: 26 VLVLRKSNFAE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKD-GVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLES 472 HSP 2 Score: 112.079 bits (279), Expect = 1.566e-24 Identity = 55/106 (51.89%), Postives = 70/106 (66.04%), Query Frame = 3
Query: 174 EEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
EE+ VLVL SNF E + +KY+LVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q YG++G+PTIK F SPK+Y R A IVN
Sbjct: 22 EEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVN 127 HSP 3 Score: 73.559 bits (179), Expect = 3.424e-12 Identity = 39/94 (41.49%), Postives = 50/94 (53.19%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSK-HSPKDYQGERT 461
V VL NF +V ++ K V VEFYAPWCGHC+ LAP W K K + MD ++ + + FPT+K F S + DY GERT
Sbjct: 369 VKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERT 462
BLAST of Protein disulfide-isomerase vs. UniProt/SwissProt
Match: sp|P05307|PDIA1_BOVIN (Protein disulfide-isomerase OS=Bos taurus OX=9913 GN=P4HB PE=1 SV=1) HSP 1 Score: 504.982 bits (1299), Expect = 9.016e-171 Identity = 247/450 (54.89%), Postives = 331/450 (73.56%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDE-MVDFEGDRTLETFKKFIDSN 1931
V+ L NFDE L + + LVEF+APWCGHCKALAPEYA AA KLK + S I+LAKVDAT E+DLA ++ ++GYPT+KFFK+G +Y GR AD I++WL+K+TGP A+TL A+AL+ES +V V+GFFKD S +AK F A+ DD+ FG+TS VF ++++ D +VLFKKFDEGRN+F G + + FI QLP+VIEF ++TA KIFGG IK H+L F+ K Y F+ +A+ F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ +++E + F L+GK+KPH+M+QE+P+DWDK PVKVLVGKNF+ VA D+ K VFVEFYAPWCGHCKQLAPIWD+LGETYKD+ +IVIAKMDSTANEVE V++ SFPT+K+FP +D ++D+ G+RTL+ FKKF++S
Sbjct: 28 VLVLHKGNFDE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAASTLSDGAAAEALVESSEVAVIGFFKDMESDSAKQFFLAAEVIDDIPFGITSNSDVFSKYQLDKD-GVVLFKKFDEGRNNFEGEVTKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYEGKLSNFKKAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQELPDDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESG 475 HSP 2 Score: 110.923 bits (276), Expect = 3.817e-24 Identity = 54/106 (50.94%), Postives = 70/106 (66.04%), Query Frame = 3
Query: 174 EEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
EE+ VLVL NF+E + +KY+LVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q YG++G+PTIK F SPK+Y R A IVN
Sbjct: 24 EEDHVLVLHKGNFDEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVN 129 HSP 3 Score: 75.0998 bits (183), Expect = 1.116e-12 Identity = 42/107 (39.25%), Postives = 57/107 (53.27%), Query Frame = 3
Query: 147 LAQCNADITEEEGVLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSK-HSPKDYQGERT 461
++Q D +++ V VL NF EV ++ K V VEFYAPWCGHC+ LAP W K K + MD ++ + + FPT+K F S + DY GERT
Sbjct: 358 MSQELPDDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERT 464
BLAST of Protein disulfide-isomerase vs. UniProt/SwissProt
Match: sp|Q2HWU2|PDIA1_MACFU (Protein disulfide-isomerase OS=Macaca fuscata fuscata OX=9543 GN=P4HB PE=2 SV=1) HSP 1 Score: 504.597 bits (1298), Expect = 1.375e-170 Identity = 246/449 (54.79%), Postives = 333/449 (74.16%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDE-MVDFEGDRTLETFKKFIDS 1928
V+ L SNF E L + + LVEF+APWCGHCKALAPEYA AA KLK + S I+LAKVDAT E+DLA ++ ++GYPT+KFF++G +Y GR AD I++WL+K+TGP A TL A++L+ES +V V+GFFKD S +AK F A++ DD+ FG+TS VF ++++ D +VLFKKFDEGRN+F G +++ FI QLP+VIEF ++TA KIFGG IK H+L F+ K Y F+T+A+ F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ +++E + F L+GK+KPH+M+QE+PEDWDK PVKVLVGKNF+ VA D++K VFVEFYAPWCGHCKQLAPIWD+LGETYKD+ +IVIAKMDSTANEVE +++ SFPT+K+FP D ++D+ G+RTL+ FKKF++S
Sbjct: 28 VLVLRKSNFAE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKD-GVVLFKKFDEGRNNFEGEVTKENLLDFIKYNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAIKVHSFPTLKFFPASVDRTVIDYNGERTLDGFKKFLES 474 HSP 2 Score: 111.694 bits (278), Expect = 1.907e-24 Identity = 55/106 (51.89%), Postives = 70/106 (66.04%), Query Frame = 3
Query: 174 EEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
EE+ VLVL SNF E + +KY+LVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q YG++G+PTIK F SPK+Y R A IVN
Sbjct: 24 EEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVN 129 HSP 3 Score: 77.0258 bits (188), Expect = 2.375e-13 Identity = 41/94 (43.62%), Postives = 51/94 (54.26%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERT 461
V VL NF EV ++NK V VEFYAPWCGHC+ LAP W K K + MD ++ + + FPT+K F S + DY GERT
Sbjct: 371 VKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAIKVHSFPTLKFFPASVDRTVIDYNGERT 464
BLAST of Protein disulfide-isomerase vs. UniProt/SwissProt
Match: sp|Q5R5B6|PDIA1_PONAB (Protein disulfide-isomerase OS=Pongo abelii OX=9601 GN=P4HB PE=2 SV=1) HSP 1 Score: 503.827 bits (1296), Expect = 2.254e-170 Identity = 246/449 (54.79%), Postives = 332/449 (73.94%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM---DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDE-MVDFEGDRTLETFKKFIDS 1928
V+ L SNF E L + + LVEF+APWCGHCKALAPEYA AA KLK + S I+LAKVDAT E+DLA ++ ++GYPT+KFF++G +Y GR AD I++WL+K+TGP A L A++L+ES +V VVGFFKD S +AK F A++ DD+ FG+TS VF ++++ D +VLFKKFDEGRN+F G +++ FI QLP+VIEF ++TA KIFGG IK H+L F+ K Y F+T+A+ F G++LF+ I++D DN R+LEFFG+ K + P+ R+I LE++M K+KP+ +++E + F L+GK+KPH+M+QE+P+DWDK PVKVLVGKNF+ VA D+ K VFVEFYAPWCGHCKQLAPIWD+LGETYKD+ +IVIAKMDSTANEVE V++ SFPT+K+FP +D ++D+ G+RTL+ FKKF++S
Sbjct: 26 VLVLRKSNFAE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATILPDGAAAESLVESSEVAVVGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKD-GVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPDDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLES 472 HSP 2 Score: 117.087 bits (292), Expect = 3.668e-26 Identity = 61/126 (48.41%), Postives = 77/126 (61.11%), Query Frame = 3
Query: 120 LSIVLFCFALAQ--CNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSIVN 479
L L C A+A C EE+ VLVL SNF E + +KY+LVEFYAPWCGHC++LAPE+ KAA LK +++ VD + S L Q YG++G+PTIK F SPK+Y R A IVN
Sbjct: 2 LRRALLCLAVAGLVCADAPEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVN 127 HSP 3 Score: 73.559 bits (179), Expect = 3.394e-12 Identity = 41/107 (38.32%), Postives = 57/107 (53.27%), Query Frame = 3
Query: 147 LAQCNADITEEEGVLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSK-HSPKDYQGERT 461
++Q D +++ V VL NF +V ++ K V VEFYAPWCGHC+ LAP W K K + MD ++ + + FPT+K F S + DY GERT
Sbjct: 356 MSQELPDDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERT 462
BLAST of Protein disulfide-isomerase vs. TrEMBL
Match: A0A1S3K0S4 (Protein disulfide-isomerase OS=Lingula unguis OX=7574 GN=LOC106177788 PE=3 SV=1) HSP 1 Score: 560.451 bits (1443), Expect = 0.000e+0 Identity = 274/447 (61.30%), Postives = 346/447 (77.40%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
DV+ L + NFD V++S + LVEF+APWCGHCKALAPEYA AA+ LKE+ S IKL K+DATVE +A KF+I+GYPTLKFF+DGK ++Y GGRT+ I++WL+KKTGPPA L VEE K I +++V VVGFFKDQ S AKAF A S DDVVFG+TS AVF + KV D+ +VLFKKFDEGRN+F G + I+TFI QLP+V+EF QE+A KIFGG IK H+L FI+KK+ + + +F +A F G+VLF+ I+TD++DN R+LEFFG+ D P+ R+I L DDMVK+KP D + + + FVQ LDGK+KPH+M++E+PEDWDKNPVKVLVG NF VA+DK+K VFVEFYAPWCGHCKQLAPIWD+LGE +KDN D+V+AKMDST NE+E ++IQSFPT+KYFPKDSDE+VD+ G+RTLE KF++S
Sbjct: 53 DVLVLGNDNFDS-VIESTKFILVEFYAPWCGHCKALAPEYAKAAKALKEEGSDIKLGKLDATVETSVAEKFEIRGYPTLKFFRDGKPVEYQGGRTSTEIVNWLKKKTGPPAKALSGVEETKEFIGAQEVAVVGFFKDQESEGAKAFLETAASMDDVVFGITSDDAVFTDLKVEKDN-VVLFKKFDEGRNNFEGELKAEAIKTFIQGNQLPLVVEFTQESAQKIFGGEIKNHILMFINKKAGDFGQKLEEFREAASGFKGKVLFIWIDTDQDDNVRILEFFGLTTADVPAIRLISLGDDMVKYKPADRNMDRDTVKTFVQDFLDGKLKPHLMSEEVPEDWDKNPVKVLVGSNFAEVAKDKTKGVFVEFYAPWCGHCKQLAPIWDKLGEHFKDNADVVVAKMDSTKNELEDIKIQSFPTLKYFPKDSDEVVDYSGERTLEALTKFLESG 497 HSP 2 Score: 125.176 bits (313), Expect = 3.082e-26 Identity = 62/126 (49.21%), Postives = 83/126 (65.87%), Query Frame = 3
Query: 111 MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVN 479
MK LS++ AD+ EEE VLVL N NF+ VIE K++LVEFYAPWCGHC++LAPE+ KAA ALK +K+G +D +S+ + + I+G+PT+K F K P +YQG RT+ IVN
Sbjct: 29 MKVLSVICLAILALTNAADVKEEEDVLVLGNDNFDSVIESTKFILVEFYAPWCGHCKALAPEYAKAAKALKEEGSDIKLGKLDATVETSVAEKFEIRGYPTLKFFRDGK--PVEYQGGRTSTEIVN 152 HSP 3 Score: 83.1889 bits (204), Expect = 8.105e-13 Identity = 45/93 (48.39%), Postives = 50/93 (53.76%), Query Frame = 3
Query: 186 VLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V VL SNF EV +K K V VEFYAPWCGHC+ LAP W K K V MD + + IQ FPT+K F K DY GERT
Sbjct: 394 VKVLVGSNFAEVAKDKTKGVFVEFYAPWCGHCKQLAPIWDKLGEHFKDNADVVVAKMDSTKNELEDIKIQSFPTLKYFPKDSDEVVDYSGERT 486
BLAST of Protein disulfide-isomerase vs. TrEMBL
Match: A0A1S3K1I5 (Protein disulfide-isomerase OS=Lingula unguis OX=7574 GN=LOC106177788 PE=3 SV=1) HSP 1 Score: 559.681 bits (1441), Expect = 0.000e+0 Identity = 274/447 (61.30%), Postives = 346/447 (77.40%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
DV+ L + NFD V++S + LVEF+APWCGHCKALAPEYA AA+ LKE+ S IKL K+DATVE +A KF+I+GYPTLKFF+DGK ++Y GGRT+ I++WL+KKTGPPA L VEE K I +++V VVGFFKDQ S AKAF A S DDVVFG+TS AVF + KV D+ +VLFKKFDEGRN+F G + I+TFI QLP+V+EF QE+A KIFGG IK H+L FI+KK+ + + +F +A F G+VLF+ I+TD++DN R+LEFFG+ D P+ R+I L DDMVK+KP D + + + FVQ LDGK+KPH+M++E+PEDWDKNPVKVLVG NF VA+DK+K VFVEFYAPWCGHCKQLAPIWD+LGE +KDN D+V+AKMDST NE+E ++IQSFPT+KYFPKDSDE+VD+ G+RTLE KF++S
Sbjct: 53 DVLVLGNDNFDS-VIESTKFILVEFYAPWCGHCKALAPEYAKAAKALKEEGSDIKLGKLDATVETSVAEKFEIRGYPTLKFFRDGKPVEYQGGRTSTEIVNWLKKKTGPPAKALSGVEETKEFIGAQEVAVVGFFKDQESEGAKAFLETAASMDDVVFGITSDDAVFTDLKVEKDN-VVLFKKFDEGRNNFEGELKAEAIKTFIQGNQLPLVVEFTQESAQKIFGGEIKNHILMFINKKAGDFGQKLEEFREAASGFKGKVLFIWIDTDQDDNVRILEFFGLTTADVPAIRLISLGDDMVKYKPADRNMDRDTVKTFVQDFLDGKLKPHLMSEEVPEDWDKNPVKVLVGSNFAEVAKDKTKGVFVEFYAPWCGHCKQLAPIWDKLGEHFKDNADVVVAKMDSTKNELEDIKIQSFPTLKYFPKDSDEVVDYSGERTLEALTKFLESG 497 HSP 2 Score: 125.176 bits (313), Expect = 3.409e-26 Identity = 62/126 (49.21%), Postives = 83/126 (65.87%), Query Frame = 3
Query: 111 MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVN 479
MK LS++ AD+ EEE VLVL N NF+ VIE K++LVEFYAPWCGHC++LAPE+ KAA ALK +K+G +D +S+ + + I+G+PT+K F K P +YQG RT+ IVN
Sbjct: 29 MKVLSVICLAILALTNAADVKEEEDVLVLGNDNFDSVIESTKFILVEFYAPWCGHCKALAPEYAKAAKALKEEGSDIKLGKLDATVETSVAEKFEIRGYPTLKFFRDGK--PVEYQGGRTSTEIVN 152 HSP 3 Score: 83.1889 bits (204), Expect = 8.646e-13 Identity = 45/93 (48.39%), Postives = 50/93 (53.76%), Query Frame = 3
Query: 186 VLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V VL SNF EV +K K V VEFYAPWCGHC+ LAP W K K V MD + + IQ FPT+K F K DY GERT
Sbjct: 394 VKVLVGSNFAEVAKDKTKGVFVEFYAPWCGHCKQLAPIWDKLGEHFKDNADVVVAKMDSTKNELEDIKIQSFPTLKYFPKDSDEVVDYSGERT 486
BLAST of Protein disulfide-isomerase vs. TrEMBL
Match: A0A1S3K0F5 (Protein disulfide-isomerase OS=Lingula unguis OX=7574 GN=LOC106177788 PE=3 SV=1) HSP 1 Score: 559.681 bits (1441), Expect = 0.000e+0 Identity = 274/447 (61.30%), Postives = 346/447 (77.40%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
DV+ L + NFD V++S + LVEF+APWCGHCKALAPEYA AA+ LKE+ S IKL K+DATVE +A KF+I+GYPTLKFF+DGK ++Y GGRT+ I++WL+KKTGPPA L VEE K I +++V VVGFFKDQ S AKAF A S DDVVFG+TS AVF + KV D+ +VLFKKFDEGRN+F G + I+TFI QLP+V+EF QE+A KIFGG IK H+L FI+KK+ + + +F +A F G+VLF+ I+TD++DN R+LEFFG+ D P+ R+I L DDMVK+KP D + + + FVQ LDGK+KPH+M++E+PEDWDKNPVKVLVG NF VA+DK+K VFVEFYAPWCGHCKQLAPIWD+LGE +KDN D+V+AKMDST NE+E ++IQSFPT+KYFPKDSDE+VD+ G+RTLE KF++S
Sbjct: 53 DVLVLGNDNFDS-VIESTKFILVEFYAPWCGHCKALAPEYAKAAKALKEEGSDIKLGKLDATVETSVAEKFEIRGYPTLKFFRDGKPVEYQGGRTSTEIVNWLKKKTGPPAKALSGVEETKEFIGAQEVAVVGFFKDQESEGAKAFLETAASMDDVVFGITSDDAVFTDLKVEKDN-VVLFKKFDEGRNNFEGELKAEAIKTFIQGNQLPLVVEFTQESAQKIFGGEIKNHILMFINKKAGDFGQKLEEFREAASGFKGKVLFIWIDTDQDDNVRILEFFGLTTADVPAIRLISLGDDMVKYKPADRNMDRDTVKTFVQDFLDGKLKPHLMSEEVPEDWDKNPVKVLVGSNFAEVAKDKTKGVFVEFYAPWCGHCKQLAPIWDKLGEHFKDNADVVVAKMDSTKNELEDIKIQSFPTLKYFPKDSDEVVDYSGERTLEALTKFLESG 497 HSP 2 Score: 125.561 bits (314), Expect = 3.012e-26 Identity = 62/126 (49.21%), Postives = 83/126 (65.87%), Query Frame = 3
Query: 111 MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVN 479
MK LS++ AD+ EEE VLVL N NF+ VIE K++LVEFYAPWCGHC++LAPE+ KAA ALK +K+G +D +S+ + + I+G+PT+K F K P +YQG RT+ IVN
Sbjct: 29 MKVLSVICLAILALTNAADVKEEEDVLVLGNDNFDSVIESTKFILVEFYAPWCGHCKALAPEYAKAAKALKEEGSDIKLGKLDATVETSVAEKFEIRGYPTLKFFRDGK--PVEYQGGRTSTEIVN 152 HSP 3 Score: 83.5741 bits (205), Expect = 7.118e-13 Identity = 45/93 (48.39%), Postives = 50/93 (53.76%), Query Frame = 3
Query: 186 VLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V VL SNF EV +K K V VEFYAPWCGHC+ LAP W K K V MD + + IQ FPT+K F K DY GERT
Sbjct: 394 VKVLVGSNFAEVAKDKTKGVFVEFYAPWCGHCKQLAPIWDKLGEHFKDNADVVVAKMDSTKNELEDIKIQSFPTLKYFPKDSDEVVDYSGERT 486
BLAST of Protein disulfide-isomerase vs. TrEMBL
Match: A0A3M7PW31 (Protein disulfide-isomerase OS=Brachionus plicatilis OX=10195 GN=BpHYR1_029450 PE=3 SV=1) HSP 1 Score: 539.65 bits (1389), Expect = 0.000e+0 Identity = 262/447 (58.61%), Postives = 338/447 (75.62%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
DV+ LT NF+E V+ S + LVEF+APWCGHCKALAPEYA AA KLK ++S I+LAKVDATV+++L +FKI+GYPTLKFF DG ++Y GGRT+D II W +KK+GP + T+ SV+E K L +V+V+G FKD +S A + +VA D F +TS++AVFDE V S + +VL KKFDEGRNDF G F ++I+ FI QLP+V EFNQETA KIFGG IK H L F SKK++ +F+ +AK F G+V+FV+I++D ++N+RV+EFFG+ K D P+ R+I L DM KFKP+ + I++ L FV+ LDGK+KPH++TQ++PEDWD PVKVLVGKNFD VARDKSK V VEFYAPWCGHCKQLAPI+DQLGE +KD+ + VIAKMDST NE+E ++IQSFPTIK FPKDSD ++DF G+R LE KF++S+
Sbjct: 25 DVLVLTSDNFEE-VVGSNKFLLVEFYAPWCGHCKALAPEYAKAAGKLKSESSEIRLAKVDATVQSELGERFKIRGYPTLKFFVDGTPLEYTGGRTSDEIIQWCKKKSGPASVTVASVDELKNLQAENEVLVLGLFKDASSEQATVYNNVAKLVDSAAFAITSEQAVFDELNVKSSEGLVLLKKFDEGRNDFEGTFDAEEIKRFIHANQLPLVTEFNQETAQKIFGGDIKVHNLLFASKKADNMEATMNEFKAAAKDFRGKVIFVLIDSDVDENERVMEFFGLKKEDAPTVRLISLGQDMTKFKPESSEITASVLTQFVRDFLDGKLKPHLLTQDVPEDWDAQPVKVLVGKNFDQVARDKSKTVLVEFYAPWCGHCKQLAPIYDQLGEKFKDSAEFVIAKMDSTVNELEDIKIQSFPTIKLFPKDSDAVIDFTGERNLEAMTKFVESH 470 HSP 2 Score: 110.153 bits (274), Expect = 1.553e-21 Identity = 52/109 (47.71%), Postives = 74/109 (67.89%), Query Frame = 3
Query: 162 ADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVN 479
+++ EEE VLVLT+ NF EV+ NK++LVEFYAPWCGHC++LAPE+ KAA LK +++ VD S LG+ + I+G+PT+K F +P +Y G RT+ I+
Sbjct: 18 SEVQEEEDVLVLTSDNFEEVVGSNKFLLVEFYAPWCGHCKALAPEYAKAAGKLKSESSEIRLAKVDATVQSELGERFKIRGYPTLKFFVDG--TPLEYTGGRTSDEIIQ 124 HSP 3 Score: 77.411 bits (189), Expect = 5.125e-11 Identity = 40/93 (43.01%), Postives = 53/93 (56.99%), Query Frame = 3
Query: 186 VLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V VL NF++V +K+K VLVEFYAPWCGHC+ LAP + + K + MD + + IQ FPTIK+F K + D+ GER
Sbjct: 367 VKVLVGKNFDQVARDKSKTVLVEFYAPWCGHCKQLAPIYDQLGEKFKDSAEFVIAKMDSTVNELEDIKIQSFPTIKLFPKDSDAVIDFTGERN 459
BLAST of Protein disulfide-isomerase vs. TrEMBL
Match: A0A1I8HRX2 (Protein disulfide-isomerase OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011389g1 PE=3 SV=1) HSP 1 Score: 534.258 bits (1375), Expect = 5.531e-180 Identity = 264/447 (59.06%), Postives = 344/447 (76.96%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXK-DDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSE-KYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDS 1928
V+ LT+ NFD L++ + LVEF+APWCGHCKALAPEYA AA++LKEKNS IKLAKVDATVE LA K+ +QGYPT+KFFK+ ++Y GGRTADTIISWLEKK GPP L SV+ A+ I K++ V+GFFKD S +AK++E A SK +D+ G+ + EFKV D +++LFKK D R +TGPF ++ F+ +LP++ EF QETAG IFGG +KKH+L F+ K E +++ + Q + A++F G+ LF+ ++ EDN+R+LEFFG+ K D P+ R I L++DMVK+KP+ I+++NL FVQSVLDGK+KPH+M+QE+P DWDK PVKVLVGKNF+ VARDK+K V VEFYAPWCGHCKQLAPIWD+LGE YK++ +IVIAKMDSTANEVE V++QSFPTIK+FP +SDE++D+EGDRTLE FKKF++S
Sbjct: 28 VLVLTEKNFDS-ALQANKYILVEFYAPWCGHCKALAPEYAKAAQQLKEKNSEIKLAKVDATVEGKLAEKYGVQGYPTIKFFKEQTPIEYSGGRTADTIISWLEKKLGPPTQRLDSVDAAQKFINDKEITVIGFFKDTTSDSAKSYEDAAFSKLEDIPCGMADSADIMAEFKVDQD-TVMLFKKTDGARIPYTGPFTADEVAKFVKNNKLPLINEFTQETAGDIFGGDVKKHLLVFLKKSDEAQFSNIMDQLKAPAEQFKGKALFIYVDIGVEDNERILEFFGLKKEDCPAVRFIALDEDMVKYKPEFTDITADNLAQFVQSVLDGKLKPHLMSQEVPADWDKEPVKVLVGKNFNEVARDKTKNVLVEFYAPWCGHCKQLAPIWDKLGEEYKNHENIVIAKMDSTANEVEDVKVQSFPTIKFFPANSDEIIDYEGDRTLEGFKKFLES 472 HSP 2 Score: 125.176 bits (313), Expect = 1.895e-26 Identity = 64/129 (49.61%), Postives = 84/129 (65.12%), Query Frame = 3
Query: 132 LFCFALAQCNA----DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKEL 497
L F+L C A ITEEEGVLVLT NF+ ++ NKY+LVEFYAPWCGHC++LAPE+ KAA LK +K+ VD L + YG+QG+PTIK F + +P +Y G RTA +I++ K+L
Sbjct: 6 LALFSLVACVAFADEAITEEEGVLVLTEKNFDSALQANKYILVEFYAPWCGHCKALAPEYAKAAQQLKEKNSEIKLAKVDATVEGKLAEKYGVQGYPTIKFF--KEQTPIEYSGGRTADTIISWLEKKL 132 HSP 3 Score: 85.5001 bits (210), Expect = 1.365e-13 Identity = 44/97 (45.36%), Postives = 56/97 (57.73%), Query Frame = 3
Query: 174 EEEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
++E V VL NFNEV +K K VLVEFYAPWCGHC+ LAP W K K + MD ++ + +Q FPTIK F + DY+G+RT
Sbjct: 366 DKEPVKVLVGKNFNEVARDKTKNVLVEFYAPWCGHCKQLAPIWDKLGEEYKNHENIVIAKMDSTANEVEDVKVQSFPTIKFFPANSDEIIDYEGDRT 462
BLAST of Protein disulfide-isomerase vs. Ensembl Cavefish
Match: p4hb (prolyl 4-hydroxylase subunit beta [Source:NCBI gene;Acc:103023413]) HSP 1 Score: 504.982 bits (1299), Expect = 9.997e-172 Identity = 249/451 (55.21%), Postives = 335/451 (74.28%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK---VMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPK-DSDEMVDFEGDRTLETFKKFIDSN 1931
DV+ L SNF+E LK+ LVEF+APWCGHCKALAPEYA AA LK + S I+L KVDAT E DLA +F ++GYPT+KFFK G+ +Y GR AD I++WL+K+TGP AA L V +A++LI +V V+GFFKD S AKAF A++ DD+ FG++S AVF +F+V+ +DS+VLFKKFDEGRN F G + + +FI QLP+VIEF ++TA KIFGG IK H+L F+ K S+ + + F +A+ F G++LF+ I++D +DN R+LEFFG+ K + P+ R+I LED+M K+KP+ + I++EN+ F + +GK+KPH+M+Q+IPEDWDK PVK LVGKNF+ V D +K VFVEFYAPWCGHCKQLAPIWDQLGE YKD+ D+VIAKMDSTANE+E V++ SFPT+K+FP + +++D+ G+RTL+ F KF++S
Sbjct: 23 DVLVLNKSNFEE-ALKAHPNILVEFYAPWCGHCKALAPEYAKAAGMLKAEGSDIRLGKVDATEEADLAQEFGVRGYPTIKFFKGGEKSSPKEYSAGRQADDIVNWLKKRTGPAAAALTEVTQAESLIADNEVAVIGFFKDVESDGAKAFIKAAEAVDDIPFGISSDDAVFSKFEVT-EDSVVLFKKFDEGRNTFDGELSKEGLLSFIKANQLPLVIEFTEQTAPKIFGGEIKSHILLFVPKASKDFQDKMDNFRKAAEGFKGKILFIFIDSDVDDNQRILEFFGLKKEECPALRLITLEDEMTKYKPEASEITAENIITFCTAFTEGKLKPHLMSQDIPEDWDKTPVKTLVGKNFEEVVFDPTKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDSKDVVIAKMDSTANEIEAVKVHSFPTLKFFPAGEEHKIIDYSGERTLDGFTKFLESG 471 HSP 2 Score: 124.02 bits (310), Expect = 2.149e-29 Identity = 60/124 (48.39%), Postives = 81/124 (65.32%), Query Frame = 3
Query: 120 LSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIVN 479
L +VL C A+++EEE VLVL SNF E ++ + +LVEFYAPWCGHC++LAPE+ KAA LK +++G VD + + L Q +G++G+PTIK F G K SPK+Y R A IVN
Sbjct: 2 LKLVLLCALAVFATAELSEEEDVLVLNKSNFEEALKAHPNILVEFYAPWCGHCKALAPEYAKAAGMLKAEGSDIRLGKVDATEEADLAQEFGVRGYPTIKFFKGGEKSSPKEYSAGRQADDIVN 125 HSP 3 Score: 76.2554 bits (186), Expect = 5.592e-14 Identity = 46/125 (36.80%), Postives = 61/125 (48.80%), Query Frame = 3
Query: 123 SIVLFCFALAQ-------CNADITEE---EGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERT 461
+I+ FC A + + DI E+ V L NF EV+ + K V VEFYAPWCGHC+ LAP W + K V MD ++ + + FPT+K F +H DY GERT
Sbjct: 336 NIITFCTAFTEGKLKPHLMSQDIPEDWDKTPVKTLVGKNFEEVVFDPTKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDSKDVVIAKMDSTANEIEAVKVHSFPTLKFFPAGEEHKIIDYSGERT 460
BLAST of Protein disulfide-isomerase vs. Ensembl Cavefish
Match: pdia2 (protein disulfide isomerase family A, member 2 [Source:ZFIN;Acc:ZDB-GENE-040426-705]) HSP 1 Score: 429.483 bits (1103), Expect = 1.484e-141 Identity = 226/454 (49.78%), Postives = 313/454 (68.94%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK---VMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPF---VDKD-IRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSD-EMVDFEGDRTLETFKKFIDS 1928
DV+ L NFD L + LVEF+APWC HC+ L P Y+ A +LK ++S I+LAKVDAT E +LA +F I G+PTLKFFK+G+ D+ G RT I WL+++ PPA LK+ ++A+ L+++ DVVVVGFF D AK F SVA + FG+TS +F ++ V+ D S+VLFKKFD+ R D + P +DKD + TFI L ++I F++E A KIF + H+L FI+ E + L + +A +F G+VLF+ I+ +EE + V+++FG++ +DTP R I D + K+ ++ TI+ + L F Q VLDGK+KPH+M+QEIPEDW+KNPVKVLVGKNF+ +A D+SK VFVEFYAPWCGHCK+LAP+W++LGE YKD+ DI+IA MDSTANEVEGV +Q FPT+K+FP ++ ++VD+ G R LETF KF+D+
Sbjct: 54 DVLVLHSVNFDR-ALSESKYLLVEFYAPWCSHCRMLEPVYSQVARQLKNESSPIRLAKVDATEEKELAEEFGIDGFPTLKFFKEGRRQNATDFTGKRTEKGIKLWLQRQVEPPATVLKNTKDAQELLDAHDVVVVGFFDDLNGEEAKVFYSVAVDMVSLNFGITSSPELFKKYNVNKD-SVVLFKKFDDKRADISLPEDGKLDKDNLVTFIQNNSLELIITFSEENADKIFSSKVHNHILLFINTTIESHNALLDDYRAAAVEFKGKVLFIKIDVNEEVS-HVMKYFGISASDTPVLRFINT-DTVKKYAMEEETINKDTLKTFCQGVLDGKIKPHLMSQEIPEDWNKNPVKVLVGKNFEHIAFDESKNVFVEFYAPWCGHCKELAPVWEKLGEKYKDHEDIIIANMDSTANEVEGVTVQGFPTLKFFPAGTERKIVDYNGKRDLETFSKFLDN 503 HSP 2 Score: 86.2705 bits (212), Expect = 3.668e-17 Identity = 40/100 (40.00%), Postives = 61/100 (61.00%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKS-KHSPKDYQGERTAGSI 473
VLVL + NF+ + ++KY+LVEFYAPWC HC+ L P + + A LK +++ VD + L + +GI GFPT+K F + + + D+ G+RT I
Sbjct: 55 VLVLHSVNFDRALSESKYLLVEFYAPWCSHCRMLEPVYSQVARQLKNESSPIRLAKVDATEEKELAEEFGIDGFPTLKFFKEGRRQNATDFTGKRTEKGI 154 HSP 3 Score: 73.9442 bits (180), Expect = 2.821e-13 Identity = 37/93 (39.78%), Postives = 53/93 (56.99%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGER 458
V VL NF + +++K V VEFYAPWCGHC+ LAP W+K K + +MD ++ + +QGFPT+K F ++ DY G+R
Sbjct: 400 VKVLVGKNFEHIAFDESKNVFVEFYAPWCGHCKELAPVWEKLGEKYKDHEDIIIANMDSTANEVEGVTVQGFPTLKFFPAGTERKIVDYNGKR 492
BLAST of Protein disulfide-isomerase vs. Ensembl Cavefish
Match: pdia4 (protein disulfide isomerase family A member 4 [Source:NCBI gene;Acc:103026638]) HSP 1 Score: 326.25 bits (835), Expect = 6.420e-101 Identity = 212/613 (34.58%), Postives = 333/613 (54.32%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALI-ESKDVVVVGFF-KDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLF-KKFDEGRNDFTGPFVDKD------IRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIIN--------TDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEV--EGVRIQSFPTIKYFPKDSDEM-VDFE-GDRTLETFKKFIDSN 1931
++ EE GVLVLT++NF+ +E VLVEFYAPWCGHC+ APE++K A LK + V VD K ++LG + + G+PTIKI K + P +Y G+R+ +IV + + E+ + + LT NFD+ V+ + + LVEF+APWCGHCK LAPEY AA++L + I LAKVDATVENDLAS+F + GYPTLK F+ GKV DY G R I+ ++ ++ GPP+ ++++++ + L+ + D ++VG F ++ + E+ +DD F T + K S ++L +KF + F KD ++ F K+ LP+V Q K + + + V+ + + + + + + +VL V ++ DEED L+ G++++ + L + K+ + +E L FV + GK+KP + +Q +P+ +K PVKV+VGK FD + DK K V +EFYAPWCGHCK+L P + L + YK+ ++VIAKMD+TAN+V + +++ FPTI + P ++ + + FE G+R +E F KFI+ +
Sbjct: 56 EVKEENGVLVLTDANFDTFMEGKDTVLVEFYAPWCGHCKQFAPEYEKIAQVLKESDPPIPVAKVDATKATALGSRFEVSGYPTIKILKKGE--PVEYDGDRSEKAIV----ERVKEVAQPDWKPPPEA------------------TLVLTKDNFDD-VVNNADIILVEFYAPWCGHCKRLAPEYEKAAKELSNRTPPILLAKVDATVENDLASRFDVTGYPTLKMFRKGKVFDYNGPREKIGIVDYMSEQAGPPSKQVQALKQIQELLKDGDDALIVGVFSSEEDAVYEIYQEACNTLRDDYKFRHTFSSEIVKFLKASPGQVVMLQPEKFRSKHEPSSYSFTIKDSTSASELQDFFKKHTLPLVGHRKQSNDAKRY--TKRPLVVVYYG------VDFSFDYRVATQFWRSKVLEVAMDFPEYTFAIADEEDYADELKSLGLSESGE-EVNVAILGEGGKKYAMEPEEFDTEVLREFVVAFKKGKLKPIIKSQPVPK--NKGPVKVIVGKTFDEIVLDKKKDVLIEFYAPWCGHCKKLEPDYLALAKKYKNEKNLVIAKMDATANDVPHDSYKVEGFPTIYFAPSNNKQNPIKFEGGERNVEGFSKFIEEH 632
BLAST of Protein disulfide-isomerase vs. Ensembl Cavefish
Match: zgc:136472 (protein disulfide-isomerase-like [Source:NCBI gene;Acc:103036532]) HSP 1 Score: 268.47 bits (685), Expect = 1.359e-80 Identity = 151/451 (33.48%), Postives = 256/451 (56.76%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMD---YGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRN-DFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPK-DSDEMVDFEGDRTLETFKKFIDS 1928
DV+ LT+SNF+ LK LV F+AP G E+A AA +LKE NS +KL +D + + DLA + P+++ + G + +++ +IISWL+++ GP A +K + + + D+V++G FKD A K F VA D+ FGVT + VF ++ +++S++L +K + + T V D+ FI +++P+V E+N +T+ KI ++ H++ F+ K + + + F+++AK+F G+VLFV+I++ E N R++E+F + K D P RM+ L D P D + + F++S L GK K + ++ +P+ WDK PVK LVG NF+ VA + K V + FY+PW + L P+W+ L E + D+ D+V+A++D TAN+V + +P IK FP ++ +V + G+ LE +F+++
Sbjct: 34 DVLVLTESNFNR-ALKLHSQLLVHFYAPLSGESLGSILEFAQAASELKEANSDVKLGGLDVSKQKDLAKTLNVTTVPSVRLYLSGDKYNPVYCPVLKSSASIISWLKRRKGPSADLIKDLSQLEKFTAEDDLVILGLFKDVEEGAVKVFYEVAADVPDLPFGVTGNEQVFSKYG-RTENSVLLLRKSKMAEHFEMTSETVKDDVILFIRIHEMPLVTEYNGKTSSKILSSVVQNHLILFMDKAGKGFKQTYRAFKSTAKEFRGKVLFVLIDSGEPRNGRIMEYFRVRKEDAPLVRMVNLTDSFQYQLPSD-LMDIQTFTDFIESYLQGKAKLKLQSEPVPDGWDKQPVKELVGMNFERVAFNHEKNVLILFYSPWSSDSRALFPLWEDLAEHFSDSEDVVVARIDITANDVNIRMMDRYPCIKVFPAVYAERVVSYSGEAKLEPIVEFVNA 481 HSP 2 Score: 63.1586 bits (152), Expect = 6.584e-10 Identity = 38/121 (31.40%), Postives = 67/121 (55.37%), Query Frame = 3
Query: 132 LFCFALAQCNAD-ITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIVN 479
L C L++ N + I E++ VLVLT SNFN ++ + +LV FYAP G E+ +AA+ LK VK+G +D+ K L + + P+++++ K++P +++ SI++
Sbjct: 16 LMCGTLSKKNDEPIQEDKDVLVLTESNFNRALKLHSQLLVHFYAPLSGESLGSILEFAQAASELKEANSDVKLGGLDVSKQKDLAKTLNVTTVPSVRLYLSGDKYNPVYCPVLKSSASIIS 136
BLAST of Protein disulfide-isomerase vs. Ensembl Cavefish
Match: zgc:136472 (protein disulfide-isomerase-like [Source:NCBI gene;Acc:103036532]) HSP 1 Score: 268.47 bits (685), Expect = 1.449e-80 Identity = 151/451 (33.48%), Postives = 256/451 (56.76%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMD---YGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRN-DFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPK-DSDEMVDFEGDRTLETFKKFIDS 1928
DV+ LT+SNF+ LK LV F+AP G E+A AA +LKE NS +KL +D + + DLA + P+++ + G + +++ +IISWL+++ GP A +K + + + D+V++G FKD A K F VA D+ FGVT + VF ++ +++S++L +K + + T V D+ FI +++P+V E+N +T+ KI ++ H++ F+ K + + + F+++AK+F G+VLFV+I++ E N R++E+F + K D P RM+ L D P D + + F++S L GK K + ++ +P+ WDK PVK LVG NF+ VA + K V + FY+PW + L P+W+ L E + D+ D+V+A++D TAN+V + +P IK FP ++ +V + G+ LE +F+++
Sbjct: 31 DVLVLTESNFNR-ALKLHSQLLVHFYAPLSGESLGSILEFAQAASELKEANSDVKLGGLDVSKQKDLAKTLNVTTVPSVRLYLSGDKYNPVYCPVLKSSASIISWLKRRKGPSADLIKDLSQLEKFTAEDDLVILGLFKDVEEGAVKVFYEVAADVPDLPFGVTGNEQVFSKYG-RTENSVLLLRKSKMAEHFEMTSETVKDDVILFIRIHEMPLVTEYNGKTSSKILSSVVQNHLILFMDKAGKGFKQTYRAFKSTAKEFRGKVLFVLIDSGEPRNGRIMEYFRVRKEDAPLVRMVNLTDSFQYQLPSD-LMDIQTFTDFIESYLQGKAKLKLQSEPVPDGWDKQPVKELVGMNFERVAFNHEKNVLILFYSPWSSDSRALFPLWEDLAEHFSDSEDVVVARIDITANDVNIRMMDRYPCIKVFPAVYAERVVSYSGEAKLEPIVEFVNA 478 HSP 2 Score: 62.3882 bits (150), Expect = 1.229e-9 Identity = 38/121 (31.40%), Postives = 67/121 (55.37%), Query Frame = 3
Query: 132 LFCFALAQCNAD-ITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIVN 479
L C L++ N + I E++ VLVLT SNFN ++ + +LV FYAP G E+ +AA+ LK VK+G +D+ K L + + P+++++ K++P +++ SI++
Sbjct: 13 LMCGTLSKKNDEPIQEDKDVLVLTESNFNRALKLHSQLLVHFYAPLSGESLGSILEFAQAASELKEANSDVKLGGLDVSKQKDLAKTLNVTTVPSVRLYLSGDKYNPVYCPVLKSSASIIS 133
BLAST of Protein disulfide-isomerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002052.1 (pep scaffold:Pmarinus_7.0:GL480798:2613:20969:-1 gene:ENSPMAG00000001848.1 transcript:ENSPMAT00000002052.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 310.842 bits (795), Expect = 1.187e-98 Identity = 168/402 (41.79%), Postives = 240/402 (59.70%), Query Frame = 3
Query: 678 CGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPI-----VIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAK-TDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFP 1856
C C L P+ A E L+E+ + ++L +D LA++F + YP F G K Y R A+ + W ++ GPPA T+ + EA+A ++S +V++GFFKD S K F +AD+ D+V FGVTS + VF ++ VS+D SIVLFKKFDEG+ FT +D + R I + + + +T +F G + H+L F + SE + EL QF+ +A F G++LFV++N+ +ND VL+ F + P+ R++++ DMVK P + ++ N+ F Q LD KP +QEIP+DW+K PVKVLVGKNFD D++K VFVEFYA WC HCK+LAP+W+QLG YKD+ +IVIAKMD+ NEV V I +P IKYFP
Sbjct: 1 CEQCSELPPKLARTVELLRERGAPVRLGSLDGETHATLATEFDVSHYPDFLLFDPGSRAKPRHYHAQREAEALAKWATRRAGPPAVTVATTAEAQAFVDSDSLVLLGFFKDTESRDLKTFLEIADTYDEVPFGVTSSEEVFAKYGVSTD-SIVLFKKFDEGQKSFTP--IDLNFRHGIKSHTAVVHGCECLCGCTVQTFPLLFSGLVNVHILLFANASSEGFPELFEQFKAAAPPFRGKILFVLLNSAVPENDNVLDLFELTDGARLPAIRLVKVNKDMVKHVPSFSDFTTANITSFCQDYLDEHNKPFSKSQEIPKDWNKRPVKVLVGKNFDEFVFDETKNVFVEFYASWCDHCKKLAPVWEQLGVQYKDHDNIVIAKMDAEKNEVAAVHIPGYPKIKYFP 399 HSP 2 Score: 61.2326 bits (147), Expect = 4.133e-10 Identity = 32/81 (39.51%), Postives = 46/81 (56.79%), Query Frame = 3
Query: 603 LTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG 845
L NFDE V + VEF+A WC HCK LAP + + K+ ++I+ +AK+DA E + + I GYP +K+F G
Sbjct: 324 LVGKNFDEFVFDETKNVFVEFYASWCDHCKKLAPVWEQLGVQYKDHDNIV-IAKMDA--EKNEVAAVHIPGYPKIKYFPAG 401 HSP 3 Score: 57.3806 bits (137), Expect = 6.949e-9 Identity = 31/78 (39.74%), Postives = 40/78 (51.28%), Query Frame = 3
Query: 186 VLVLTNSNFNE-VIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF 416
V VL NF+E V ++ K V VEFYA WC HC+ LAP W++ K + MD + I G+P IK F
Sbjct: 321 VKVLVGKNFDEFVFDETKNVFVEFYASWCDHCKKLAPVWEQLGVQYKDHDNIVIAKMDAEKNEVAAVHIPGYPKIKYF 398
BLAST of Protein disulfide-isomerase vs. Ensembl Sea Lamprey
Match: pdia4 (protein disulfide isomerase family A, member 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5493]) HSP 1 Score: 303.523 bits (776), Expect = 4.054e-93 Identity = 207/612 (33.82%), Postives = 321/612 (52.45%), Query Frame = 3
Query: 168 ITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIES-KDVVVVGFFKD-QXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVL----FKKFDEGRN---DFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVL--------FVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEV--EGVRIQSFPTIKYFPKDS-DEMVDF-EGDRTLETFKKFIDSN 1931
+ EE GVL+L + NF+ + VL+EFYAPWCGHC+ A E++K A L + V VD L + + + G+PTIKI K + DY G+RT +I+ A +KE+ +S V+ LT+SNFDE+V + LVEF+APWCGHCK LAPEY AA++L + I LAKVDATVE++L +F + GYPTLK F+ GK DY G R I+S + ++ GP + LKS+++ +++ DVV++G FK+ Q A E+ + +++ F T ++ + + K +VL F+ E + D D D++ F++K+ +P+V + K + S + V+ F + + T+ + + +VL F E+D + L G+A++ I L+ K+ ++ L FV S GK+K + +Q +P+ +K PVKV+VG+ F+ + D SK V +EFYAPWCGHCK L PI+ LG+ YK + +VIAKMD+T N++ + + + FPTI + P + D + F +G R LE+ KFI+ +
Sbjct: 52 VKEENGVLILKDLNFDFFVAGKDTVLLEFYAPWCGHCKEFASEYEKIAKELAENDPPIPVAKVDATVAVHLAERFSVSGYPTIKILKKGQAV--DYDGDRTQDAII-AKVKEV---------------------ASPDWKPPPEAVLTLTESNFDEIV-NDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKNTPPIPLAKVDATVESELGKRFGVSGYPTLKLFRKGKAYDYNGPREQYGIVSHMIEQAGPASQELKSMKQLNGFVQNGDDVVILGVFKNEQDVALQLFQETGNNVREEFKFYHTFEENIKNHLKAVDGQVVVLHPERFQSKYEPKQFTFDIQADSTDSDLKAFVEKHSVPLVGQRKPSNEAKRY-ASRRPLVVVFYG------VDFSFDYRTATQFWRSKVLEVAKDFPKFTFAIASEDDYSKELSDLGLAESGEEVNAAI-LDASGKKYAMAPEEFDADVLRDFVISFEKGKLKAVVKSQPLPKS-NKGPVKVVVGRTFEQMVLDNSKDVLIEFYAPWCGHCKSLEPIYKDLGKKYKGHKGLVIAKMDATVNDIPHDSYKAEGFPTIYFSPSGAKDRPIKFADGSRDLESLSKFIEKH 629
BLAST of Protein disulfide-isomerase vs. Ensembl Sea Lamprey
Match: pdia3 (protein disulfide isomerase family A, member 3 [Source:ZFIN;Acc:ZDB-GENE-031002-9]) HSP 1 Score: 245.358 bits (625), Expect = 7.896e-73 Identity = 166/460 (36.09%), Postives = 253/460 (55.00%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVM-DYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXX-XXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFK------KFDEGRNDFT-GPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAEL-KGQFETSAKKFL--GQVLFVIINTDEEDNDRVLEFFGMAKT--DTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEG-VRIQSFPTIKYFPKDS-DEMVDFEGDRTLETFKKFI 1922
DV++LTD++F V + LVEFFAPWCGHCK LAPEY AA KLK + + LAKVD T + +KF + GYPTLK F+DG++ DY G RTAD I+ +L+K+ GP + LK+VE+ + + D +VGFFKD +S A + + +D+ F ++ +AV D K ++++IV+F+ KF+E + + + I+ FI L I + G + K+ K + + + AKKF+ G L I + +D + L +G+ D P + + D + ++ T ++L F+Q DGK+K H+ ++ IPED D PVKV+V + FD + D+SK V +EFYAPWCGHCK L P + +L + K++ +I+IAKMD+TAN+V ++ FPTI + P S D +EG R + F ++
Sbjct: 26 DVLDLTDADFASKV-AGHDMVLVEFFAPWCGHCKRLAPEYEKAATKLK---NTVALAKVDCTANTETCNKFGVSGYPTLKIFRDGELSSDYDGPRTADGIVGFLKKQAGPSSVELKTVEDLEKFVGDNDPSIVGFFKDASSDAQTEFMKVASSQRDNYRFAHSNVQAVLD--KQGAEEAIVVFRPLRLASKFEETQVTYAEDSYSSAKIKAFIQDNILGICPHLTDDNKDLFKGKDLLVAYYDVDYTKNPKGSNYWRNRVMKVAKKFIEEGHTLSYAI-ANHKDFGQQLSDYGLEHVSGDVPVVAIKKSNGDKYILR-EEFTRDGKSLENFLQDYFDGKLKKHLKSEPIPEDND-GPVKVVVAEIFDELVNDESKDVLIEFYAPWCGHCKNLEPKYLELADKLKNDPNILIAKMDATANDVPAQYDVRGFPTIYFSPMGSKDSPKKYEGGREVSDFISYL 476 HSP 2 Score: 95.9005 bits (237), Expect = 6.200e-21 Identity = 46/97 (47.42%), Postives = 62/97 (63.92%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
VL LT+++F + + VLVEF+APWCGHC+ LAPE++KAAT LK V + VD + +G+ G+PT+KIF + S DY G RTA IV
Sbjct: 27 VLDLTDADFASKVAGHDMVLVEFFAPWCGHCKRLAPEYEKAATKLKNTVALAKVDCTANTETCNKFGVSGYPTLKIFRDGELS-SDYDGPRTADGIV 122 HSP 3 Score: 68.9366 bits (167), Expect = 1.873e-12 Identity = 39/106 (36.79%), Postives = 62/106 (58.49%), Query Frame = 3
Query: 186 VLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSS-LGQPYGIQGFPTIKIFGK-SKHSPKDYQGERTAGSIVNAALKE 494
V V+ F+E++ +++K VL+EFYAPWCGHC++L P++ + A LK + MD ++ + Y ++GFPTI SK SPK Y+G R ++ +E
Sbjct: 374 VKVVVAEIFDELVNDESKDVLIEFYAPWCGHCKNLEPKYLELADKLKNDPNILIAKMDATANDVPAQYDVRGFPTIYFSPMGSKDSPKKYEGGREVSDFISYLKRE 479
BLAST of Protein disulfide-isomerase vs. Ensembl Sea Lamprey
Match: pdia6 (protein disulfide isomerase family A, member 6 [Source:ZFIN;Acc:ZDB-GENE-030131-879]) HSP 1 Score: 230.335 bits (586), Expect = 5.566e-68 Identity = 124/268 (46.27%), Postives = 174/268 (64.93%), Query Frame = 3
Query: 108 KMKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFN-EVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXX-XXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLK-EKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG--KVMDYGGGRTADTIISW 896
++K ++ +A A + + V+ LT SNFN EV++ N LVEFYAPWCGHCQ L PEWKKAATALKG+VK+GAVD D++ LG Y ++GFPTIKIFG +K+ P+D+ G RT IV+ AL L LV+ RLGG G S + ++ G+ V + +++ E W+VEFFAPWCGHCK L PE+A+AA ++K + N +KLA VDATV +L+++F I+G+PT+K F+ G + +Y GGRT I+++
Sbjct: 1 EVKVFGALVLGVLVAGAQALYSPSDDVIELTASNFNREVVQSNDLWLVEFYAPWCGHCQRLIPEWKKAATALKGIVKLGAVDADEHKGLGSTYNVRGFPTIKIFGINKNKPEDFNGGRTTQGIVDGALGALRSLVKDRLGGKGGSDSGKQNINTFSGNGFCKRKPVSFSIITTNKITFTPSETWIVEFFAPWCGHCKKLEPEWAAAATEVKAQTNGRVKLAAVDATVNQELSNRFGIRGFPTIKVFRKGEDEPQEYQGGRTKSDIVAY 268 HSP 2 Score: 94.3597 bits (233), Expect = 1.273e-20 Identity = 45/87 (51.72%), Postives = 57/87 (65.52%), Query Frame = 3
Query: 243 LVEFYAPWCGHCQSLAPEWKKAATALK----GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALK 491
+VEF+APWCGHC+ L PEW AAT +K G VK+ AVD L +GI+GFPTIK+F K + P++YQG RT IV AL+
Sbjct: 185 IVEFFAPWCGHCKKLEPEWAAAATEVKAQTNGRVKLAAVDATVNQELSNRFGIRGFPTIKVFRKGEDEPQEYQGGRTKSDIVAYALE 271 HSP 3 Score: 69.3218 bits (168), Expect = 1.361e-12 Identity = 35/81 (43.21%), Postives = 48/81 (59.26%), Query Frame = 3
Query: 1668 SKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTD--IVIAKMDSTANEVEGVR--IQSFPTIKYFPKDSDEMVDFEGDRT 1898
S+ VEF+APWCGHCK+L P W K T+ + +A +D+T N+ R I+ FPTIK F K DE +++G RT
Sbjct: 181 SETWIVEFFAPWCGHCKKLEPEWAAAATEVKAQTNGRVKLAAVDATVNQELSNRFGIRGFPTIKVFRKGEDEPQEYQGGRT 261 HSP 4 Score: 62.7734 bits (151), Expect = 1.369e-10 Identity = 30/92 (32.61%), Postives = 42/92 (45.65%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLE 1904
L NF+ + VEFYAPWCGHC++L P W + K + D ++ FPTIK F + ++ DF G RT +
Sbjct: 30 LTASNFNREVVQSNDLWLVEFYAPWCGHCQRLIPEWKKAATALKGIVKLGAVDADEHKGLGSTYNVRGFPTIKIFGINKNKPEDFNGGRTTQ 121
BLAST of Protein disulfide-isomerase vs. Ensembl Sea Lamprey
Match: p4hb (prolyl 4-hydroxylase, beta polypeptide [Source:ZFIN;Acc:ZDB-GENE-080610-1]) HSP 1 Score: 185.652 bits (470), Expect = 1.317e-54 Identity = 96/194 (49.48%), Postives = 128/194 (65.98%), Query Frame = 3
Query: 669 APWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQ 1241
APWCGHCKAL PE+A AA LK + S I+L KVDAT E +LA ++ ++GYPT+KFFKDG +Y GR AD I++WL+K+TGP A TL IES +VVVVGFFKD + AK F AD DD+ FG+ S F +++VS + ++LFKKFDEGRN + G + +R F+ +LP++IEF +
Sbjct: 1 APWCGHCKALGPEFAKAAGTLKAEGSAIRLGKVDATEETELAKEYGVRGYPTIKFFKDGDAKNAKEYAAGRQADDIVNWLKKRTGPSATTLDDAAATAKFIESAEVVVVGFFKDVEAEDAKVFLKAADGIDDIAFGLASSDDAFTQYEVSK-EGVILFKKFDEGRNVYEGELSKEGLRKFVKSNELPLLIEFTE 193 HSP 2 Score: 74.7146 bits (182), Expect = 1.123e-15 Identity = 36/79 (45.57%), Postives = 52/79 (65.82%), Query Frame = 3
Query: 258 APWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIF--GKSKHSPKDYQGERTAGSIVN 479
APWCGHC++L PE+ KAA LK +++G VD + + L + YG++G+PTIK F G +K++ K+Y R A IVN
Sbjct: 1 APWCGHCKALGPEFAKAAGTLKAEGSAIRLGKVDATEETELAKEYGVRGYPTIKFFKDGDAKNA-KEYAAGRQADDIVN 78 HSP 3 Score: 48.521 bits (114), Expect = 1.212e-6 Identity = 26/62 (41.94%), Postives = 38/62 (61.29%), Query Frame = 3
Query: 1695 APWCGHCKQLAPIWDQLGETYK-DNTDIVIAKMDSTANEVEGVR---IQSFPTIKYFPKDSD 1868
APWCGHCK L P + + T K + + I + K+D+T E E + ++ +PTIK+F KD D
Sbjct: 1 APWCGHCKALGPEFAKAAGTLKAEGSAIRLGKVDAT-EETELAKEYGVRGYPTIKFF-KDGD 60
BLAST of Protein disulfide-isomerase vs. Ensembl Yeast
Match: PDI1 (Protein disulfide isomerase; multifunctional oxidoreductase of the ER lumen, essential for disulfide bond formation in secretory and cell-surface proteins, processing of non-native disulfide bonds; Ero1p activator; complexes with exomannosidase, Mnl1p to facilitate the recognition of misfolded glycoproteins and the trimming of glycan Man8GlcNAc2 to Man7GlcNAc2 on substrates, thereby accelerating ERAD; PDI1 has a paralog, EUG1, that arose from the whole genome duplication [Source:SGD;Acc:S000000548]) HSP 1 Score: 201.445 bits (511), Expect = 7.623e-57 Identity = 159/464 (34.27%), Postives = 239/464 (51.51%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKV---MDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEG--RNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHV-LSFI-SKKSEKYAELKGQFETSAKKFLGQVLFVIINTD----EEDNDRVLEFFGMAK----TDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNT-DIVIAKMDSTANEVEGVRIQSFPTIKYFP--KDSDEMVDFEGDRTLETFKKFIDSN 1931
VV+L +F+E + +S + L EFFAPWCGHCK +APEY AAE L EKN I LA++D T DL + I G+P+LK FK+ V +DY G RTA+ I+ ++ K++ P A + + A V+V K A A + +D F V+++ A D+FK+S I L DE N D D+ +K+ + + E G +F ++ + L ++ E+ E K F AKK G + FV I+ N + E F + T+ + + QL ++ D + S+ + V+ L G P + +QEI E+ D + V LVGKN D + D K V V +YAPWCGHCK+LAP + +L +TY + T D++IAK+D T N+V GV I+ +PTI +P K S+ +V ++G R+L++ FI N
Sbjct: 34 VVKLATDSFNEYI-QSHDLVLAEFFAPWCGHCKNMAPEYVKAAETLVEKN--ITLAQIDCTENQDLCMEHNIPGFPSLKIFKNSDVNNSIDYEGPRTAEAIVQFMIKQSQPAVAVVADLPAYLANETFVTPVIVQSGKIDADFNATFYSMANKHFNDYDF-VSAENAD-DDFKLS----IYLPSAMDEPVVYNGKKADIADADV---FEKWLQVEALPYFGEIDGSVFAQYVESGLPLGYLFYNDEEELEEYKPLFTELAKKNRGLMNFVSIDARKFGRHAGNLNMKEQFPLFAIHDMTEDLKYGLPQLSEEAFDELSDKIVLESKAIESLVKDFLKGDASPIVKSQEIFENQDSS-VFQLVGKNHDEIVNDPKKDVLVLYYAPWCGHCKRLAPTYQELADTYANATSDVLIAKLDHTENDVRGVVIEGYPTIVLYPGGKKSESVV-YQGSRSLDSLFDFIKEN 483 HSP 2 Score: 92.4337 bits (228), Expect = 4.839e-20 Identity = 47/118 (39.83%), Postives = 72/118 (61.02%), Query Frame = 3
Query: 147 LAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAA-TALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSK-HSPKDYQGERTAGSIVNAALKE 494
AQ A E+ V+ L +FNE I+ + VL EF+APWCGHC+++APE+ KAA T ++ + + +D + L + I GFP++KIF S ++ DY+G RTA +IV +K+
Sbjct: 21 FAQQEAVAPEDSAVVKLATDSFNEYIQSHDLVLAEFFAPWCGHCKNMAPEYVKAAETLVEKNITLAQIDCTENQDLCMEHNIPGFPSLKIFKNSDVNNSIDYEGPRTAEAIVQFMIKQ 138 HSP 3 Score: 61.6178 bits (148), Expect = 2.453e-10 Identity = 35/104 (33.65%), Postives = 55/104 (52.88%), Query Frame = 3
Query: 171 TEEEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAA-TALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSI 473
++ V L N +E++ + K VLV +YAPWCGHC+ LAP +++ A T V +D + + I+G+PTI ++ G K YQG R+ S+
Sbjct: 373 NQDSSVFQLVGKNHDEIVNDPKKDVLVLYYAPWCGHCKRLAPTYQELADTYANATSDVLIAKLDHTENDVRGVVIEGYPTIVLYPGGKKSESVVYQGSRSLDSL 476
BLAST of Protein disulfide-isomerase vs. Ensembl Yeast
Match: EUG1 (Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER [Source:SGD;Acc:S000002926]) HSP 1 Score: 112.849 bits (281), Expect = 1.128e-26 Identity = 118/466 (25.32%), Postives = 212/466 (45.49%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMD---YGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKS-EKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDM---VKFKPDDATISSE-----NLNLFVQSVLD---GKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLG------ETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPK-DSDEMVDFEGDRTLETFKKFI 1922
D++ LT+ F + +S LVEFFAPWC H + L P AA LKE N + + ++D + + + I YPTLK FK+G++ D Y G + D I ++ + L S +E + +E+ + VV ++ ++D V + + + + +LF D + G V + ++ LP + + K ++ + S++ E Y +L Q K+ GQ+ F+ +N+ + + F M + P F + + +++ + P++ E + ++ VQ V D G KP + ++EIP++ N K+ VGK D + D K V V++YA WC H K+ API++++ E+ +D I+IA++DS AN++ + +PTI +P ++ + + F R LE +FI
Sbjct: 34 DLLVLTEKKFKSFI-ESHPLVLVEFFAPWCLHSQILRPHLEEAASILKEHN--VPVVQIDCEANSMVCLQQTINTYPTLKIFKNGRIFDGQVYRGVKITDEITQYMIQLYEASVIYLNSEDEIQPYLENATLPVVINRGLTGLNETYQEVALDLAEDYVFLSLLDSEDKSLSIHLPNTTEPILF---DGNVDSLVGNSVA--LTQWLKVVILPYFTDIEPDLFPKYISSNLPLAYFFYTSEEELEDYTDLFTQL---GKENRGQINFIALNSTMFPHH--VRFLNM-REQFPLFAIHNMINNLKYGLPQLPEEEYAKLEKPQPLDRDMIVQLVKDYREGTAKPIVKSEEIPKEQKSNVYKI-VGKTHDDIVHDDDKDVLVKYYATWCIHSKRFAPIYEEIANVLASDESVRDK--ILIAEVDSGANDILSFPVTGYPTIALYPAGNNSKPIIFNKIRNLEDVFEFI 482 HSP 2 Score: 73.1738 bits (178), Expect = 6.505e-14 Identity = 43/107 (40.19%), Postives = 58/107 (54.21%), Query Frame = 3
Query: 123 SIVLFC----FALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK-GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSK 428
+IV FC F LA+ +A T +LVLT F IE + VLVEF+APWC H Q L P ++AA+ LK V V +D + S + I +PT+KIF +
Sbjct: 10 AIVSFCLFASFTLAENSARATPGSDLLVLTEKKFKSFIESHPLVLVEFFAPWCLHSQILRPHLEEAASILKEHNVPVVQIDCEANSMVCLQQTINTYPTLKIFKNGR 116
BLAST of Protein disulfide-isomerase vs. Ensembl Yeast
Match: MPD1 (Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation [Source:SGD;Acc:S000005814]) HSP 1 Score: 86.2705 bits (212), Expect = 9.315e-19 Identity = 47/125 (37.60%), Postives = 67/125 (53.60%), Query Frame = 3
Query: 195 LTNSNFNEVIEKNKYV-LVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAV--DMDKYSSLGQPYGIQGFPTIKIFGKSK----------------HSPKDYQGERTAGSIVNAALKELDELVR 512
LT +F++ I Y LVEFYAPWCGHC+ L+ ++KAA L GVV+V AV D++K +L Y + GFPT+ +F K H+ + Y G RT IV+ +L + V+
Sbjct: 34 LTPKSFDKAIHNTNYTSLVEFYAPWCGHCKKLSSTFRKAAKRLDGVVQVAAVNCDLNKNKALCAKYDVNGFPTLMVFRPPKIDLSKPIDNAKKSFSAHANEVYSGARTLAPIVDFSLSRIRSYVK 158 HSP 2 Score: 69.3218 bits (168), Expect = 3.260e-13 Identity = 31/88 (35.23%), Postives = 55/88 (62.50%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVEND--LASKFKIQGYPTLKFFKDGKV 851
+ ELT +FD+ + + LVEF+APWCGHCK L+ + AA++L + ++++A V+ + + L +K+ + G+PTL F+ K+
Sbjct: 31 ISELTPKSFDKAIHNTNYTSLVEFYAPWCGHCKKLSSTFRKAAKRL---DGVVQVAAVNCDLNKNKALCAKYDVNGFPTLMVFRPPKI 115 HSP 3 Score: 51.9878 bits (123), Expect = 1.799e-7 Identity = 25/86 (29.07%), Postives = 41/86 (47.67%), Query Frame = 3
Query: 1605 WDKNP-VKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVR--IQSFPTIKYF 1853
+D +P + L K+FD + + VEFYAPWCGHCK+L+ + + + + D N+ + + FPT+ F
Sbjct: 25 YDSDPHISELTPKSFDKAIHNTNYTSLVEFYAPWCGHCKKLSSTFRKAAKRLDGVVQVAAVNCDLNKNKALCAKYDVNGFPTLMVF 110
BLAST of Protein disulfide-isomerase vs. Ensembl Yeast
Match: TRX2 (Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx1p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases under DNA replication stress; TRX2 has a paralog, TRX1, that arose from the whole genome duplication [Source:SGD;Acc:S000003441]) HSP 1 Score: 54.6842 bits (130), Expect = 1.070e-9 Identity = 29/79 (36.71%), Postives = 43/79 (54.43%), Query Frame = 3
Query: 612 SNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK 848
S +D + ++ +V+FFA WCG CK +AP EK E+ S K+D +D+A K ++ PTL F+K GK
Sbjct: 9 SEYDSALASGDKLVVVDFFATWCGPCKMIAPMI----EKFAEQYSDAAFYKLDVDEVSDVAQKAEVSSMPTLIFYKGGK 83 HSP 2 Score: 47.7506 bits (112), Expect = 2.798e-7 Identity = 21/73 (28.77%), Postives = 37/73 (50.68%), Query Frame = 3
Query: 1635 GKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYF 1853
+DS K V V+F+A WCG CK +AP+ ++ E Y D + +D ++ + + S PT+ ++
Sbjct: 8 ASEYDSALASGDKLVVVDFFATWCGPCKMIAPMIEKFAEQYSDAAFYKL-DVDEVSDVAQKAEVSSMPTLIFY 79
BLAST of Protein disulfide-isomerase vs. Ensembl Yeast
Match: TRX1 (Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx2p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases iunder DNA replication stress; TRX1 has a paralog, TRX2, that arose from the whole genome duplication [Source:SGD;Acc:S000004033]) HSP 1 Score: 49.6766 bits (117), Expect = 5.984e-8 Identity = 29/81 (35.80%), Postives = 43/81 (53.09%), Query Frame = 3
Query: 606 TDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK 848
T S FD + + ++ +V+F+A WCG CK +AP EK E+ K+D D+A K ++ PTL FK+GK
Sbjct: 7 TASEFDSAIAQ-DKLVVVDFYATWCGPCKMIAPMI----EKFSEQYPQADFYKLDVDELGDVAQKNEVSAMPTLLLFKNGK 82 HSP 2 Score: 47.3654 bits (111), Expect = 3.519e-7 Identity = 25/81 (30.86%), Postives = 41/81 (50.62%), Query Frame = 3
Query: 198 TNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPK 440
T S F+ I ++K V+V+FYA WCG C+ +AP +K + +D+D+ + Q + PT+ +F K K
Sbjct: 7 TASEFDSAIAQDKLVVVDFYATWCGPCKMIAPMIEKFSEQYPQ-ADFYKLDVDELGDVAQKNEVSAMPTLLLFKNGKEVAK 86 HSP 3 Score: 45.0542 bits (105), Expect = 2.417e-6 Identity = 25/71 (35.21%), Postives = 38/71 (53.52%), Query Frame = 3
Query: 1644 FDS-VARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYF 1853
FDS +A+DK V V+FYA WCG CK +AP+ ++ E Y D +D + + + + PT+ F
Sbjct: 11 FDSAIAQDK--LVVVDFYATWCGPCKMIAPMIEKFSEQYP-QADFYKLDVDELGDVAQKNEVSAMPTLLLF 78
BLAST of Protein disulfide-isomerase vs. Ensembl Nematostella
Match: EDO36830 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHH5]) HSP 1 Score: 526.168 bits (1354), Expect = 0.000e+0 Identity = 273/447 (61.07%), Postives = 333/447 (74.50%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQL-EDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDS 1928
DV+ LT+ NFDE V ++ LVEF+APWCGHCKALAPEYA AA +LK + S IKLAKVDAT E L KF++QGYPT+KFFKDGK +Y GGRTA I+SWL KKTGPPA L + + K I +K+V VVGFF D+ S AAKAF S AD DDV FG+ S KA+ E KV D IVLFKKFDEGRND+ G + + I+ F+ QLP+V EF+ ETA KIFGG +K H+L F +K S+ + F AK F G+VLFV +NT+ EDN R++EFFG+ ++ P+ R+I L +DDM K+KP A I+SEN+ FVQ+ LD K+KPH+++ EIPEDWD PVKVL GKNFD VAR+K K VFVEFYAPWCGHCKQLAPIWDQLGE YKD+ DIV+AKMDSTANEVEGV++ SFPTIKYFPK+ E VD+ G RTL+ F KF++S
Sbjct: 25 DVLVLTEKNFDEAVAANKH-VLVEFYAPWCGHCKALAPEYAKAAGQLKSEKSEIKLAKVDATAETKLGEKFQVQGYPTIKFFKDGKPSEYAGGRTAPEIVSWLNKKTGPPAKDLATADAMKDFI-TKEVAVVGFFTDKESDAAKAFLSAADGIDDVEFGIVSDKAIASEHKVEGD-KIVLFKKFDEGRNDYDGEYDFEKIQQFVKANQLPLVTEFSDETAPKIFGGDVKHHILLFTNKTSDGFKATHEAFTGGAKDFKGKVLFVYVNTEVEDNQRIVEFFGIQSSELPTIRLINLADDDMTKYKPTAAEITSENVKEFVQAFLDKKLKPHLLSAEIPEDWDSKPVKVLCGKNFDEVARNKDKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHADIVVAKMDSTANEVEGVKVHSFPTIKYFPKEG-EAVDYNGGRTLDDFVKFLES 467 HSP 2 Score: 114.775 bits (286), Expect = 6.392e-27 Identity = 57/108 (52.78%), Postives = 73/108 (67.59%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL---KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVN 479
+I EEE VLVLT NF+E + NK+VLVEFYAPWCGHC++LAPE+ KAA L K +K+ VD + LG+ + +QG+PTIK F K P +Y G RTA IV+
Sbjct: 19 EIKEEEDVLVLTEKNFDEAVAANKHVLVEFYAPWCGHCKALAPEYAKAAGQLKSEKSEIKLAKVDATAETKLGEKFQVQGYPTIKFFKDGK--PSEYAGGRTAPEIVS 124 HSP 3 Score: 74.7146 bits (182), Expect = 5.475e-14 Identity = 41/98 (41.84%), Postives = 51/98 (52.04%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIE-KNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
V VL NF+EV K+K V VEFYAPWCGHC+ LAP W + K + MD ++ + + FPTIK F K + DY G RT V
Sbjct: 366 VKVLCGKNFDEVARNKDKNVFVEFYAPWCGHCKQLAPIWDQLGEKYKDHADIVVAKMDSTANEVEGVKVHSFPTIKYFPKEGEAV-DYNGGRTLDDFV 462
BLAST of Protein disulfide-isomerase vs. Ensembl Nematostella
Match: EDO32930 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STM8]) HSP 1 Score: 316.62 bits (810), Expect = 9.821e-98 Identity = 214/622 (34.41%), Postives = 319/622 (51.29%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKD-VVVVGFFK-DQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFIS-----KKSEKYA-----------------ELKGQFETS----AKKFLGQVLFVIINTDEEDNDRVLEFFGMAKT-DTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGV-RIQSFPTIKYFP--KDSDEMVDFEGDRTLETFKKFID 1925
++ EE+ VLVL + NF+ VIE+N +LVEFYAPWCGHC+SLAPE+ KAA +K V +D S + Q + + G+PT+KIF K +P +Y+G R IV K+ D + + LT NF E V+ E LVEFFAPWCGHCK LAPEY AA++L++ + I LA VDAT+E++LA K+++QGYPTLK F+ GK +Y G R I S++ + GP + L S++ + ++ KD V ++GFF + E+ D +DD F T A F + SIVLF+ F+ KY+ + ++ + G K + + K +KYA + QF S K + F I N EE++++ L+ FG+A++ + + E + PD+ S ++L FV+ G +KP + +Q +P+ +K PV V+VGK FD + D K V +EFYAPWCGHCK L P + +LG+ ++++ +IVIAK+D+TAN+V ++ FPTI YF KD + F+G R L+ KF++
Sbjct: 56 EVKEEDDVLVLNSKNFDRVIEENNIILVEFYAPWCGHCKSLAPEYAKAAKKMKLNDPPVPFAKMDATVASDIAQRFDVSGYPTLKIFRKG--TPYEYEGPREESGIVEYMKKQSDPNWKPPPVA----------------------ALTLTKENFTE-VVNRESLMLVEFFAPWCGHCKQLAPEYEKAAQELQKNDPPIPLAIVDATIESELAQKYEVQGYPTLKVFRKGKATEYKGQRDQYGIASYMRSQVGPSSRILSSLKAVQDFMKEKDDVTIMGFFDGEDDKMLESYLEANNDVRDDYPFAHTFDAAAKKHFGIKKS-SIVLFQP-----------------ERFLSKYEPKHFVYEGKDLSPAALQGFYKDKRVPLVGLYTSYDKDKKYAARPLCIVYYGVDFGFDNRVATQFWRSKVIEVAKDHRDITFAIAN--EEESEQELKDFGLAESGEEVNVGCFDKEGRKFRMDPDEEEFSEDSLREFVEEFKAGNLKPIIKSQPVPKS-NKEPVTVVVGKTFDEIVNDPKKDVLIEFYAPWCGHCKALEPTFKKLGKHFRNDKNIVIAKIDATANDVPSTYAVEGFPTI-YFATSKDKKNPIKFDGGRELKDLIKFVE 630 HSP 2 Score: 77.411 bits (189), Expect = 1.165e-14 Identity = 40/101 (39.60%), Postives = 64/101 (63.37%), Query Frame = 3
Query: 618 FDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFF--KDGK-VMDYGGGRTADTIISWLEKKT 911
FDE+V ++ L+EF+APWCGHCKAL P + + + +I+ +AK+DAT ND+ S + ++G+PT+ F KD K + + GGR +I ++E+K
Sbjct: 535 FDEIVNDPKKDVLIEFYAPWCGHCKALEPTFKKLGKHFRNDKNIV-IAKIDATA-NDVPSTYAVEGFPTIYFATSKDKKNPIKFDGGRELKDLIKFVEEKA 633
BLAST of Protein disulfide-isomerase vs. Ensembl Nematostella
Match: EDO37155 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGL1]) HSP 1 Score: 258.455 bits (659), Expect = 2.320e-78 Identity = 140/243 (57.61%), Postives = 175/243 (72.02%), Query Frame = 3
Query: 186 VLVLTNSNFN-EVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG-----KVMDYGGGRTADTIISW 896
V+ LT +NFN VI + +VEFYAPWCGHC++LAPEW KAATALKGVVKVGAVDMD + S+G PY I+GFPTIKIFG +K+SP+DY G+RTA IV+AA++ E V R+ G SSS GGS G+ DVVELTD+NF++ VL S++ WLVEFFAPWCGHC+ LAPE+A AA +LK K +K+ +DATV AS++++QGYPT+K F G V DY GGRTA II +
Sbjct: 14 VVELTANNFNNRVINDDAVWMVEFYAPWCGHCKALAPEWMKAATALKGVVKVGAVDMDVHQSVGGPYNIRGFPTIKIFGANKNSPQDYNGQRTAQGIVDAAMRAAQEAVSQRM-SGGGRSSSGGGGRRGGSGGNKEDVVELTDTNFEKEVLNSKDLWLVEFFAPWCGHCQRLAPEWAKAATELKGK---VKVGALDATVHTVTASRYQVQGYPTIKVFAAGIKNSHSVEDYQGGRTASDIIQY 252 HSP 2 Score: 118.242 bits (295), Expect = 2.703e-28 Identity = 59/108 (54.63%), Postives = 74/108 (68.52%), Query Frame = 3
Query: 177 EEGVLVLTNSNFN-EVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFG---KSKHSPKDYQGERTAGSIVNAAL 488
+E V+ LT++NF EV+ LVEF+APWCGHCQ LAPEW KAAT LKG VKVGA+D ++ Y +QG+PTIK+F K+ HS +DYQG RTA I+ AL
Sbjct: 147 KEDVVELTDTNFEKEVLNSKDLWLVEFFAPWCGHCQRLAPEWAKAATELKGKVKVGALDATVHTVTASRYQVQGYPTIKVFAAGIKNSHSVEDYQGGRTASDIIQYAL 254 HSP 3 Score: 101.679 bits (252), Expect = 7.716e-23 Identity = 48/102 (47.06%), Postives = 64/102 (62.75%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK--VMDYGGGRTADTII 890
DVVELT +NF+ V+ + W+VEF+APWCGHCKALAPE+ AA LK ++K+ VD V + + I+G+PT+K F K DY G RTA I+
Sbjct: 13 DVVELTANNFNNRVINDDAVWMVEFYAPWCGHCKALAPEWMKAATALK---GVVKVGAVDMDVHQSVGGPYNIRGFPTIKIFGANKNSPQDYNGQRTAQGIV 111 HSP 4 Score: 67.781 bits (164), Expect = 7.321e-12 Identity = 33/92 (35.87%), Postives = 44/92 (47.83%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLE 1904
L NF++ + VEFYAPWCGHCK LAP W + K + MD + I+ FPTIK F + + D+ G RT +
Sbjct: 17 LTANNFNNRVINDDAVWMVEFYAPWCGHCKALAPEWMKAATALKGVVKVGAVDMDVHQSVGGPYNIRGFPTIKIFGANKNSPQDYNGQRTAQ 108 HSP 5 Score: 67.0106 bits (162), Expect = 1.308e-11 Identity = 36/102 (35.29%), Postives = 54/102 (52.94%), Query Frame = 3
Query: 1608 DKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVR--IQSFPTIKYFP---KDSDEMVDFEGDRT 1898
+K V L NF+ + VEF+APWCGHC++LAP W + K + + +D+T + V R +Q +PTIK F K+S + D++G RT
Sbjct: 146 NKEDVVELTDTNFEKEVLNSKDLWLVEFFAPWCGHCQRLAPEWAKAATELKGK--VKVGALDATVHTVTASRYQVQGYPTIKVFAAGIKNSHSVEDYQGGRT 245
BLAST of Protein disulfide-isomerase vs. Ensembl Nematostella
Match: EDO31634 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXD7]) HSP 1 Score: 226.868 bits (577), Expect = 5.573e-66 Identity = 158/455 (34.73%), Postives = 233/455 (51.21%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDAT-VENDLASKFKIQGYPTLKFFKDGKVM-DYGGGRTADTIISWLEKKTGPPAATLKSVEE-AKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFK------KFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEV-EGVRIQSFPTIKYFPKDSDEMVD-FEGDRTLETFKKFI 1922
DV++L DSNF V ++ LVEFFAPWCGHCK LAPEY +AAE LK+ + + LAKVD T D SK+ + GYPTLK F++G++ DY G R + II +++K+ GP + +KSV+ K L +++ VVVGF + + +D F S V +++ + IV+F+ K + +TG D I+ FI+ V + + + F + KK+ K ++ KF D +RVL G+ PS + D K S ++ F++ G +KPH+ ++ +PE D PVKV+VG+NF + D +K V +EFYAPWCGHCK L P +++LGE +D DIVIAKMD+TAN+ +Q FPTI + P ++ E + +EG R + F FI
Sbjct: 19 DVLDLGDSNFKSGVA-GKDIMLVEFFAPWCGHCKRLAPEYETAAEALKKNDPPVPLAKVDCTEAGKDTCSKYGVSGYPTLKIFRNGEMSKDYDGPRDSSGIIRYMKKQAGPSSVEIKSVDHLEKKLDDAESNVVVGFLDGDDDLKNAFMRTANEMRDSFSFAHASVTDVLEKYNYK--NQIVVFRPKHLHTKLEPSFIAYTGSPDDFYIKKFINAKAHGRVGQRTPDNEEQ-FPKPLCVVFFDVDWKKNAKGSKYWRNRVLKVTKFFDDKAMNFAIASFSDYERVLSDIGVTDKANPSAVVYNDAGDKFLMK---EKFSVDSFKQFLEDYFAGSLKPHIKSEPLPESND-GPVKVVVGENFKEIVNDPTKDVLIEFYAPWCGHCKSLEPKYNELGEKLQDVKDIVIAKMDATANDAPPNFSVQGFPTIYWAPANNKENPEKYEGGREVSDFVDFI 465 HSP 2 Score: 82.0333 bits (201), Expect = 2.667e-16 Identity = 49/134 (36.57%), Postives = 71/134 (52.99%), Query Frame = 3
Query: 111 MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK------GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKE 494
M Y + + F + ++D VL L +SNF + +LVEF+APWCGHC+ LAPE++ AA ALK + KV + K + YG+ G+PT+KIF + S KDY G R + I+ K+
Sbjct: 1 MLYSTAFVLLFVGSTLSSD------VLDLGDSNFKSGVAGKDIMLVEFFAPWCGHCKRLAPEYETAAEALKKNDPPVPLAKVDCTEAGKDTC--SKYGVSGYPTLKIFRNGEMS-KDYDGPRDSSGIIRYMKKQ 125 HSP 3 Score: 77.7962 bits (190), Expect = 5.608e-15 Identity = 40/105 (38.10%), Postives = 61/105 (58.10%), Query Frame = 3
Query: 615 NFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMD---YGGGRTADTIISWLEKKTGPP 920
NF E+V + L+EF+APWCGHCK+L P+Y EKL++ I+ +AK+DAT ND F +QG+PT+ + + Y GGR + ++++K P
Sbjct: 370 NFKEIVNDPTKDVLIEFYAPWCGHCKSLEPKYNELGEKLQDVKDIV-IAKMDATA-NDAPPNFSVQGFPTIYWAPANNKENPEKYEGGREVSDFVDFIKRKATKP 472 HSP 4 Score: 73.559 bits (179), Expect = 1.166e-13 Identity = 38/101 (37.62%), Postives = 58/101 (57.43%), Query Frame = 3
Query: 186 VLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQP-YGIQGFPTIK-IFGKSKHSPKDYQGERTAGSIVN 479
V V+ NF E++ + K VL+EFYAPWCGHC+SL P++ + L+ V + MD ++ P + +QGFPTI +K +P+ Y+G R V+
Sbjct: 363 VKVVVGENFKEIVNDPTKDVLIEFYAPWCGHCKSLEPKYNELGEKLQDVKDIVIAKMDATANDAPPNFSVQGFPTIYWAPANNKENPEKYEGGREVSDFVD 463
BLAST of Protein disulfide-isomerase vs. Ensembl Nematostella
Match: EDO47340 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMG9]) HSP 1 Score: 173.711 bits (439), Expect = 6.023e-48 Identity = 111/301 (36.88%), Postives = 155/301 (51.50%), Query Frame = 3
Query: 129 VLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL--KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDEL--VRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLK-EKNSIIKLAKVDATVENDLASKFKIQGYPTLKFF----KDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQ 1004
VLF L + +T+ + V+ LT NF+EV+ K+ LVEFYAPWCGHC+ LAP +++ A V + VD D LG + ++GFPTIK F K +P++Y G R +N +K ++E VR R+ V +L +SNFD++V + LVEFFAPWCGHCK LAP Y E K E N +I AKVDA + L K+ + GYPTLKFF KDG+ +Y GR + + ++ +K G + E I + D V F K++
Sbjct: 6 VLFKLFLLFSISSLTQGK-VIDLTKDNFDEVVNGEKFALVEFYAPWCGHCKQLAPTYEQLGEAYTQSSDVIIAKVDADGDRDLGSRFDVKGFPTIKYFPKGSTTPEEYNGGRD----INDFIKFIEEKTGVRGRVPVIPSA------------------VADLDESNFDKIVKNPDNNVLVEFFAPWCGHCKNLAPVYEKVGEAFKNEPNCVI--AKVDADAHSALGQKYGVSGYPTLKFFSKTNKDGE--EYSSGRDEQSFVDFMNEKCGTKRTPGGGLNEQAGRINAFDGFAVEFMKNK 279 HSP 2 Score: 104.76 bits (260), Expect = 3.086e-24 Identity = 50/101 (49.50%), Postives = 66/101 (65.35%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVR--IQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFID 1925
L NFD V + K VEFYAPWCGHCKQLAP ++QLGE Y ++D++IAK+D+ + G R ++ FPTIKYFPK S ++ G R + F KFI+
Sbjct: 27 LTKDNFDEVV-NGEKFALVEFYAPWCGHCKQLAPTYEQLGEAYTQSSDVIIAKVDADGDRDLGSRFDVKGFPTIKYFPKGSTTPEEYNGGRDINDFIKFIE 126 HSP 3 Score: 88.1965 bits (217), Expect = 1.032e-18 Identity = 36/97 (37.11%), Postives = 64/97 (65.98%), Query Frame = 3
Query: 1641 NFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVR--IQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFID 1925
NFD + ++ V VEF+APWCGHCK LAP+++++GE +K+ + VIAK+D+ A+ G + + +PT+K+F K + + ++ R ++F F++
Sbjct: 149 NFDKIVKNPDNNVLVEFFAPWCGHCKNLAPVYEKVGEAFKNEPNCVIAKVDADAHSALGQKYGVSGYPTLKFFSKTNKDGEEYSSGRDEQSFVDFMN 245
BLAST of Protein disulfide-isomerase vs. Ensembl Medaka
Match: p4hb (prolyl 4-hydroxylase subunit beta [Source:NCBI gene;Acc:101155742]) HSP 1 Score: 501.516 bits (1290), Expect = 2.247e-170 Identity = 247/451 (54.77%), Postives = 331/451 (73.39%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSD-EMVDFEGDRTLETFKKFIDSN 1931
DV+ L SNFDE L++ LVEF+APWCGHCKALAPEYA AA LK + S I+L KVDAT E +LA +F ++GYPT+KFFK G +Y GR AD +++WL+K+TGP +L V +A++LI +V V+GFFKD S AKA+E A++ DDV F +TS AV+ +F+VS D +VLFKKFDEGRN F G + F+ QLP+VIEF ++TA KIFGG IK H+L F+ K + + E QF+ +A F GQ+LF+ I++D +DN R+LEFFG+ K + P+ R+I LED+M K+KP+ I++E++ F ++GK+KPH+M+Q+IPEDWDKNPVKVLVGKNF+ VA D K VF+EFYAPWCGHCKQLAPIWD+LGE YKD++DIV+AKMDSTANE+E V++ SFPT+K+FP + +++D+ G+RTLE F KF++S
Sbjct: 23 DVLVLKKSNFDE-ALQAHPNILVEFYAPWCGHCKALAPEYAKAAGMLKAEGSQIRLGKVDATEETELAQEFGVRGYPTIKFFKGGDKESPKEYSAGRQADDMVNWLKKRTGPAVTSLTEVTDAESLIADNEVAVIGFFKDANSDDAKAYEKAAEAMDDVPFAITSSDAVYSKFEVSKD-GVVLFKKFDEGRNTFDGELTKDGLLAFVKANQLPLVIEFTEQTAPKIFGGDIKSHILMFLPKAASDFQEKMEQFKKAAAGFKGQILFIFIDSDVDDNQRILEFFGLKKEECPAIRLITLEDEMTKYKPESKDITAESIVAFCTQFVEGKLKPHLMSQDIPEDWDKNPVKVLVGKNFEEVAFDPKKNVFIEFYAPWCGHCKQLAPIWDKLGEKYKDSSDIVVAKMDSTANEIESVKVHSFPTLKFFPAGEERQVIDYNGERTLEGFTKFLESG 471 HSP 2 Score: 122.479 bits (306), Expect = 6.626e-29 Identity = 58/124 (46.77%), Postives = 80/124 (64.52%), Query Frame = 3
Query: 120 LSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKG---VVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIVN 479
L ++ C A+I EEE VLVL SNF+E ++ + +LVEFYAPWCGHC++LAPE+ KAA LK +++G VD + + L Q +G++G+PTIK F G K SPK+Y R A +VN
Sbjct: 2 LKFLMLCTLAVASRAEIAEEEDVLVLKKSNFDEALQAHPNILVEFYAPWCGHCKALAPEYAKAAGMLKAEGSQIRLGKVDATEETELAQEFGVRGYPTIKFFKGGDKESPKEYSAGRQADDMVN 125 HSP 3 Score: 74.3294 bits (181), Expect = 1.950e-13 Identity = 46/125 (36.80%), Postives = 59/125 (47.20%), Query Frame = 3
Query: 123 SIVLFCFALAQ-------CNADITEE---EGVLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERT 461
SIV FC + + DI E+ V VL NF EV + K V +EFYAPWCGHC+ LAP W K K + MD ++ + + FPT+K F + DY GERT
Sbjct: 336 SIVAFCTQFVEGKLKPHLMSQDIPEDWDKNPVKVLVGKNFEEVAFDPKKNVFIEFYAPWCGHCKQLAPIWDKLGEKYKDSSDIVVAKMDSTANEIESVKVHSFPTLKFFPAGEERQVIDYNGERT 460
BLAST of Protein disulfide-isomerase vs. Ensembl Medaka
Match: pdia2 (protein disulfide-isomerase [Source:NCBI gene;Acc:101164281]) HSP 1 Score: 379.022 bits (972), Expect = 3.292e-122 Identity = 209/453 (46.14%), Postives = 293/453 (64.68%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK----DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFK-PDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDS 1928
++ L +NFD L +VEF+APWCG+C+ P YA AA LKE+ S ++LAKVDA E +LA +F + +PT+K F +G + ++Y G RT I+ WL++++GP AA L S + A I + +V VVGFF + S AA F+ VA DV F VT+ VF E++V+++ +VLFKKFD+GR DF DK ++ FI + L +++ FNQET+ KIF ++ H L FI+ E L + +T A++F G+VL+++I+ + VL +FG+ + D+P+ RMI + D KFK + TI S L Q V+D +P++ ++EIPEDWDK PVKVLV KNF++VA D +K VFVEFYAPWCGHCK+LAPIW+QLGE Y D+ DI+IAKMD+TANEVE V I FPT+KYFP E+ + G+R LET KF+D+
Sbjct: 67 IMVLHINNFDR-ALSETRHLMVEFYAPWCGYCRRFEPIYAEAAGMLKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIVQWLKRRSGPGAADLNSADSAAEFINTHNVSVVGFFDNLESEAAAVFKEVAFDLTDVEFAVTASPEVFKEYEVTAN-KVVLFKKFDDGRADFELSEEDKLDKHNLTNFIKENSLELIVPFNQETSDKIFTSRVRLHCLLFINSTVESQMSLLEESKTVAREFKGKVLYILIDMTTAVS-HVLNYFGVLEKDSPTVRMINM-DTQKKFKIASEMTIDS--LRQLSQEVVDDTAEPYLRSEEIPEDWDKGPVKVLVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATANEVESVAIDGFPTLKYFPAGDKEV--YTGNRDLETLSKFLDN 511 HSP 2 Score: 86.2705 bits (212), Expect = 4.414e-17 Identity = 38/108 (35.19%), Postives = 66/108 (61.11%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIV 476
+I EE+G++VL +NF+ + + ++++VEFYAPWCG+C+ P + +AA LK +++ VD + L + + + FPT+K+F + P +Y G+RT IV
Sbjct: 60 EIEEEQGIMVLHINNFDRALSETRHLMVEFYAPWCGYCRRFEPIYAEAAGMLKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIV 167 HSP 3 Score: 63.929 bits (154), Expect = 3.563e-10 Identity = 32/78 (41.03%), Postives = 42/78 (53.85%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF 416
V VL NF V ++ K V VEFYAPWCGHC+ LAP W++ + MD ++ + I GFPT+K F
Sbjct: 411 VKVLVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATANEVESVAIDGFPTLKYF 488
BLAST of Protein disulfide-isomerase vs. Ensembl Medaka
Match: pdia2 (protein disulfide-isomerase [Source:NCBI gene;Acc:101164281]) HSP 1 Score: 377.867 bits (969), Expect = 2.781e-121 Identity = 204/452 (45.13%), Postives = 289/452 (63.94%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK----DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDS 1928
++ L +NFD L +VEF+APWCG+C+ P YA AA LKE+ S ++LAKVDA E +LA +F + +PT+K F +G + ++Y G RT I+ WL++++GP AA L S + A I + +V VVGFF + S AA F+ VA DV F VT+ VF E++V+++ +VLFKKFD+GR DF DK ++ FI + L +++ FNQET+ KIF ++ H L FI+ E L + +T A++F G+VL+++I+ + VL +FG+ + D+P+ RMI + D KFK + V+D +P++ ++EIPEDWDK PVKVLV KNF++VA D +K VFVEFYAPWCGHCK+LAPIW+QLGE Y D+ DI+IAKMD+TANEVE V I FPT+KYFP E++ + G+R LET KF+D+
Sbjct: 71 IMVLHINNFDR-ALSETRHLMVEFYAPWCGYCRRFEPIYAEAAGMLKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIVQWLKRRSGPGAADLNSADSAAEFINTHNVSVVGFFDNLESEAAAVFKEVAFDLTDVEFAVTASPEVFKEYEVTAN-KVVLFKKFDDGRADFELSEEDKLDKHNLTNFIKENSLELIVPFNQETSDKIFTSRVRLHCLLFINSTVESQMSLLEESKTVAREFKGKVLYILIDMTTAVS-HVLNYFGVLEKDSPTVRMINM-DTQKKFKIE---------------VVDDTAEPYLRSEEIPEDWDKGPVKVLVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATANEVESVAIDGFPTLKYFPAGDKEVISYTGNRDLETLSKFLDN 503 HSP 2 Score: 86.6557 bits (213), Expect = 2.916e-17 Identity = 38/108 (35.19%), Postives = 66/108 (61.11%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIV 476
+I EE+G++VL +NF+ + + ++++VEFYAPWCG+C+ P + +AA LK +++ VD + L + + + FPT+K+F + P +Y G+RT IV
Sbjct: 64 EIEEEQGIMVLHINNFDRALSETRHLMVEFYAPWCGYCRRFEPIYAEAAGMLKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIV 171 HSP 3 Score: 67.3958 bits (163), Expect = 3.903e-11 Identity = 35/92 (38.04%), Postives = 45/92 (48.91%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGER 458
V VL NF V ++ K V VEFYAPWCGHC+ LAP W++ + MD ++ + I GFPT+K F Y G R
Sbjct: 401 VKVLVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATANEVESVAIDGFPTLKYFPAGDKEVISYTGNR 492
BLAST of Protein disulfide-isomerase vs. Ensembl Medaka
Match: pdia2 (protein disulfide-isomerase [Source:NCBI gene;Acc:101164281]) HSP 1 Score: 346.28 bits (887), Expect = 6.336e-111 Identity = 190/408 (46.57%), Postives = 268/408 (65.69%), Query Frame = 3
Query: 729 LKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDK----DIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFK-PDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDS 1928
LKE+ S ++LAKVDA E +LA +F + +PT+K F +G + ++Y G RT I+ WL++++GP AA L S + A I + +V VVGFF + S AA F+ VA DV F VT+ VF E++V+++ +VLFKKFD+GR DF DK ++ FI + L +++ FNQET+ KIF ++ H L FI+ E L + +T A++F G+VL+++I+ + VL +FG+ + D+P+ RMI + D KFK + TI S L Q V+D +P++ ++EIPEDWDK PVKVLV KNF++VA D +K VFVEFYAPWCGHCK+LAPIW+QLGE Y D+ DI+IAKMD+TANEVE V I FPT+KYFP E++ + G+R LET KF+D+
Sbjct: 2 LKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIVQWLKRRSGPGAADLNSADSAAEFINTHNVSVVGFFDNLESEAAAVFKEVAFDLTDVEFAVTASPEVFKEYEVTAN-KVVLFKKFDDGRADFELSEEDKLDKHNLTNFIKENSLELIVPFNQETSDKIFTSRVRLHCLLFINSTVESQMSLLEESKTVAREFKGKVLYILIDMTTAVS-HVLNYFGVLEKDSPTVRMINM-DTQKKFKIASEMTIDS--LRQLSQEVVDDTAEPYLRSEEIPEDWDKGPVKVLVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATANEVESVAIDGFPTLKYFPAGDKEVISYTGNRDLETLSKFLDN 404 HSP 2 Score: 73.9442 bits (180), Expect = 1.986e-13 Identity = 41/102 (40.20%), Postives = 61/102 (59.80%), Query Frame = 3
Query: 603 LTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG--KVMDYGGGRTADTIISWLE 902
L NF+ + + + VEF+APWCGHCK LAP + E + + II +AK+DAT N++ S I G+PTLK+F G +V+ Y G R +T+ +L+
Sbjct: 305 LVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDII-IAKMDATA-NEVES-VAIDGFPTLKYFPAGDKEVISYTGNRDLETLSKFLD 403 HSP 3 Score: 67.781 bits (164), Expect = 1.908e-11 Identity = 35/92 (38.04%), Postives = 45/92 (48.91%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGER 458
V VL NF V ++ K V VEFYAPWCGHC+ LAP W++ + MD ++ + I GFPT+K F Y G R
Sbjct: 302 VKVLVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATANEVESVAIDGFPTLKYFPAGDKEVISYTGNR 393
BLAST of Protein disulfide-isomerase vs. Ensembl Medaka
Match: pdia2 (protein disulfide-isomerase [Source:NCBI gene;Acc:101164281]) HSP 1 Score: 341.273 bits (874), Expect = 1.350e-107 Identity = 194/448 (43.30%), Postives = 276/448 (61.61%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDG---KVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDS 1928
++ L +NFD L +VEF+APWCG+C+ P YA AA LKE+ S ++LAKVDA E +LA +F + +PT+K F +G + ++Y G RT I+ WL++++GP AA L S + A I + +V VVGFF + S AA F+ VA DV F VT+ VF E++V+++ +VLFKK +R K ++ Q A KIF ++ H L FI+ E L + +T A++F G+VL+++I+ + VL +FG+ + D+P+ RMI + D KFK I+SE + + S+ +P++ ++EIPEDWDK PVKVLV KNF++VA D +K VFVEFYAPWCGHCK+LAPIW+QLGE Y D+ DI+IAKMD+TANEVE V I FPT+KYFP E++ + G+R LET KF+D+
Sbjct: 71 IMVLHINNFDR-ALSETRHLMVEFYAPWCGYCRRFEPIYAEAAGMLKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIVQWLKRRSGPGAADLNSADSAAEFINTHNVSVVGFFDNLESEAAAVFKEVAFDLTDVEFAVTASPEVFKEYEVTAN-KVVLFKK----------------VRL---KPSSGLLSTERQCNADKIFTSRVRLHCLLFINSTVESQMSLLEESKTVAREFKGKVLYILIDMTTAVS-HVLNYFGVLEKDSPTVRMINM-DTQKKFK-----IASE---MTIDSLRQLSQEPYLRSEEIPEDWDKGPVKVLVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATANEVESVAIDGFPTLKYFPAGDKEVISYTGNRDLETLSKFLDN 487 HSP 2 Score: 86.6557 bits (213), Expect = 3.469e-17 Identity = 38/108 (35.19%), Postives = 66/108 (61.11%), Query Frame = 3
Query: 165 DITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIF-GKSKHSPKDYQGERTAGSIV 476
+I EE+G++VL +NF+ + + ++++VEFYAPWCG+C+ P + +AA LK +++ VD + L + + + FPT+K+F + P +Y G+RT IV
Sbjct: 64 EIEEEQGIMVLHINNFDRALSETRHLMVEFYAPWCGYCRRFEPIYAEAAGMLKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIV 171 HSP 3 Score: 66.6254 bits (161), Expect = 5.336e-11 Identity = 35/92 (38.04%), Postives = 45/92 (48.91%), Query Frame = 3
Query: 186 VLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGER 458
V VL NF V ++ K V VEFYAPWCGHC+ LAP W++ + MD ++ + I GFPT+K F Y G R
Sbjct: 385 VKVLVAKNFETVAMDPTKNVFVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATANEVESVAIDGFPTLKYFPAGDKEVISYTGNR 476
BLAST of Protein disulfide-isomerase vs. Planmine SMEST
Match: SMESG000023147.1 (SMESG000023147.1) HSP 1 Score: 842.417 bits (2175), Expect = 0.000e+0 Identity = 433/446 (97.09%), Postives = 441/446 (98.88%), Query Frame = 3
Query: 594 VVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 1931
V+ LT+SNF+E++ K++ LVEF+APWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQASAAAKAFESVADSKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN
Sbjct: 26 VLVLTNSNFNEVIEKNKYV-LVEFYAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQASAAAKAFESVADSKDDVVFGVTSQKAVFDEFKVSSDDSIVLFKKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFIDSN 470 HSP 2 Score: 169.859 bits (429), Expect = 4.695e-45 Identity = 83/126 (65.87%), Postives = 96/126 (76.19%), Query Frame = 3
Query: 111 MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALK---GVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVN 479
MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHC++LAPE+ AA LK ++K+ VD + L + IQG+PT+K F K DY G RTA +I++
Sbjct: 1 MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK--VMDYGGGRTADTIIS 124 HSP 3 Score: 79.337 bits (194), Expect = 4.022e-15 Identity = 40/93 (43.01%), Postives = 52/93 (55.91%), Query Frame = 3
Query: 186 VLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
V VL NF+ V +K+K V VEFYAPWCGHC+ LAP W + K + MD ++ + IQ FPTIK F K D++G+RT
Sbjct: 367 VKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRT 459
BLAST of Protein disulfide-isomerase vs. Planmine SMEST
Match: SMESG000017020.1 (SMESG000017020.1) HSP 1 Score: 330.487 bits (846), Expect = 2.644e-105 Identity = 198/295 (67.12%), Postives = 221/295 (74.92%), Query Frame = 3
Query: 144 ALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRSRLXXXXXXXXXXXXXXXXXXXXXXXDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFF----KDGKVMDYGGGRTADTIISWLEKKT--GPPAATLKSV---EEAKALIESKDVVVVGFFKD 1001
AL N D+ E LT SNF+++ + VEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQG+RTAGSIVNAALKELDELVRSRLGGASGGSSSHERKSSGGSSGSGGDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCK L P + AA +L+ K +KL +DATV AS++ +QGYPT+KFF K G DY GGR++ I+ W K PA +K + E K+ E + VV +
Sbjct: 18 ALYSSNDDVVE------LTPSNFDDMKNSDSLWFVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGDRTAGSIVNAALKELDELVRSRLGGASGGSSSHERKSSGGSSGSGGDVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKNLKPHWDQAASELRGK---VKLGALDATVHTATASRYGVQGYPTIKFFPAGVKSGDSQDYDGGRSSSDIVRWASDKVVEQLPAPEIKEILNQEILKSACEDHQLCVVAVLPN 303 HSP 2 Score: 75.0998 bits (183), Expect = 7.156e-14 Identity = 37/90 (41.11%), Postives = 48/90 (53.33%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRT 1898
L NFD + ++ FVEFYAPWCGHC+ LAP W + K + MD ++ + IQ FPTIK F K D++GDRT
Sbjct: 29 LTPSNFDDM-KNSDSLWFVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGDRT 117 HSP 3 Score: 71.2478 bits (173), Expect = 1.049e-12 Identity = 34/94 (36.17%), Postives = 50/94 (53.19%), Query Frame = 3
Query: 1629 LVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVR--IQSFPTIKYFPKD--SDEMVDFEGDRT 1898
L NFD + + VEF+APWCGHCK L P WDQ + + + +D+T + R +Q +PTIK+FP S + D++G R+
Sbjct: 165 LTDSNFDEMVLKSEEPWLVEFFAPWCGHCKNLKPHWDQAASELRGK--VKLGALDATVHTATASRYGVQGYPTIKFFPAGVKSGDSQDYDGGRS 256
BLAST of Protein disulfide-isomerase vs. Planmine SMEST
Match: SMESG000069732.1 (SMESG000069732.1) HSP 1 Score: 259.225 bits (661), Expect = 9.869e-78 Identity = 150/446 (33.63%), Postives = 246/446 (55.16%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXXXXXXXXXKDDVVFGVTSQKAVFDEFKVSSDDS-IVLFKKFDEGRNDF-TGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAELKGQFETSAKKFLGQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKFI 1922
DV + + F+E++ +P L+ F+APWC + L P + +AA+ L + S IKL KVD TVE DLA+KFKI+ YPT+ FK+ + + Y TI++WL K ++ S + + + DV+ V F A+ DDV F T +K +F ++D + +L +K D F G +D+ F+ ++P V+ ++ + GK+F S + F+ +K+ E + E +A ++ FV+ + + +++ F + ++ P + + + F P I+ + ++ F+ V +GK+ + +Q IPE+W+ PVK+LVGKNF+ VA +K K VFVEFYAPWC +CKQL PIW++L + D D++IAK+D T N+++ I+SFPT+K F KDS+E+VD+ G RT E K+F+
Sbjct: 26 DVFVVNNETFEELI-NDHKPILINFYAPWCKFSQKLLPHFENAAKNLLQMESRIKLGKVDVTVETDLANKFKIKSYPTIILFKEDRQIPYSKAHHHLTILNWLISKVDKLIISVNSTDSLDEVRQKTDVLFVI----NNDIMGDTFVGAAELIDDVQFA-TMEKHLFHSLFSNTDQTKAILIRKIDNNLIPFGKGEINQEDVIKFVRSNEIPSVVRYSFDIGGKVFQNS--HRFMHFMVFMDDKFEEAHKELEEAAINH-PEITFVMTDVTDNTTSSLIKHFNVDLSNLPHLVLSDAKHKRL-FLPGFNEINGKRIDQFLMDVKNGKIGQFLKSQNIPENWNSEPVKILVGKNFEEVALNKQKTVFVEFYAPWCNYCKQLEPIWERLAKELLDVPDLIIAKLDKTENDIKNFEIKSFPTLKLFLKDSNEVVDYSGPRTRERLKEFL 461 HSP 2 Score: 78.1814 bits (191), Expect = 9.143e-15 Identity = 45/139 (32.37%), Postives = 71/139 (51.08%), Query Frame = 3
Query: 111 MKYLSIVLFCFALAQCNADITE-EEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATAL---KGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIVNAALKELDELVRS 515
MK+ L F L D E E V V+ N F E+I +K +L+ FYAPWC Q L P ++ AA L + +K+G VD+ + L + I+ +PTI +F + + P Y +I+N + ++D+L+ S
Sbjct: 1 MKFFCRFLLIFFLPAAIFDELEIENDVFVVNNETFEELINDHKPILINFYAPWCKFSQKLLPHFENAAKNLLQMESRIKLGKVDVTVETDLANKFKIKSYPTIILFKEDRQIP--YSKAHHHLTILNWLISKVDKLIIS 137 HSP 3 Score: 78.1814 bits (191), Expect = 9.143e-15 Identity = 39/95 (41.05%), Postives = 54/95 (56.84%), Query Frame = 3
Query: 180 EGVLVLTNSNFNEV-IEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERT 461
E V +L NF EV + K K V VEFYAPWC +C+ L P W++ A L V + +DK + + + I+ FPT+K+F K + DY G RT
Sbjct: 359 EPVKILVGKNFEEVALNKQKTVFVEFYAPWCNYCKQLEPIWERLAKELLDVPDLIIAKLDKTENDIKNFEIKSFPTLKLFLKDSNEVVDYSGPRT 453
BLAST of Protein disulfide-isomerase vs. Planmine SMEST
Match: SMESG000041085.1 (SMESG000041085.1) HSP 1 Score: 240.35 bits (612), Expect = 1.127e-70 Identity = 166/465 (35.70%), Postives = 250/465 (53.76%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK-VMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXX-XXXXXXXKDDVVFGVTSQKAVFDEF---KVSSDDSIVLF------KKFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAE----LKGQFETSAKKFL--GQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEG-VRIQSFPTIKYFPKDSDEMV-DFEGDRTLETFKKFIDS 1928
DV+E DS+F++ V K LVEF+APWCGHCK LAPEY AA LK+++ I +AKVD T E K + GYPTLK FK+G+ DY G R+AD II ++ K+GP + LK+ E S D VVVGFF ++S KA +D F T FDE K++ ++IV+F KF++ + + G + +RT++++ + + T + + + +++ + EK + + + AKKF+ G+ + I+ +E N + E+ G+ D + ++ KFK A S ENL F+ L+ KV ++ ++ IPE D+ PVKV+V KNFD + D +K V +EFYAPWCGHCK LAP +++L + +DIVIAKMD+TAN+V + FPTI + PK+S + R ++ K+I S
Sbjct: 4 DVLEFKDSSFEQSV-KEHSVILVEFYAPWCGHCKKLAPEYEIAATALKKQDPPIAIAKVDCTAETKTCEKHGVSGYPTLKVFKNGQFAFDYSGERSADGIIKYMVSKSGPSSKELKTEENFNKFTASADPVVVGFFTSESSDLYKAFLSVAEKLNEDFRFAHT-----FDETLSTKLNYKNNIVIFHSKKMANKFEDTQVKYKGDASEHQMRTWVEENIMGLA---GHRTHSNVKYFAKRPLIVALYNVDYEKNPKGTNYWRNRILKVAKKFIDSGKSIHFAISNVQEMNQELSEY-GIESPDFSKVYITARDERNQKFKMM-ADFSMENLEKFINDFLEEKVPVYLKSESIPEKNDE-PVKVVVAKNFDEIVNDPTKDVLIEFYAPWCGHCKSLAPKYEELAKKLSAESDIVIAKMDATANDVPSPYEVSGFPTIYFAPKNSKHSPKSYSSGREVDDMLKYIAS 456 HSP 2 Score: 89.3521 bits (220), Expect = 2.089e-18 Identity = 42/100 (42.00%), Postives = 65/100 (65.00%), Query Frame = 3
Query: 186 VLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
VL +S+F + ++++ +LVEFYAPWCGHC+ LAPE++ AATALK + + VD + + +G+ G+PT+K+F K+ DY GER+A I+
Sbjct: 5 VLEFKDSSFEQSVKEHSVILVEFYAPWCGHCKKLAPEYEIAATALKKQDPPIAIAKVDCTAETKTCEKHGVSGYPTLKVF-KNGQFAFDYSGERSADGII 103 HSP 3 Score: 84.7297 bits (208), Expect = 6.215e-17 Identity = 49/124 (39.52%), Postives = 68/124 (54.84%), Query Frame = 3
Query: 177 EEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSS-LGQPYGIQGFPTIKIFGK-SKHSPKDYQGERTA-------GSIVNAALKELDELVRSR 518
+E V V+ NF+E++ + K VL+EFYAPWCGHC+SLAP++++ A L + MD ++ + PY + GFPTI K SKHSPK Y R S ++ LK D R R
Sbjct: 349 DEPVKVVVAKNFDEIVNDPTKDVLIEFYAPWCGHCKSLAPKYEELAKKLSAESDIVIAKMDATANDVPSPYEVSGFPTIYFAPKNSKHSPKSYSSGREVDDMLKYIASEASSPLKNFDRQGRDR 472
BLAST of Protein disulfide-isomerase vs. Planmine SMEST
Match: SMESG000041085.1 (SMESG000041085.1) HSP 1 Score: 239.965 bits (611), Expect = 1.817e-70 Identity = 166/465 (35.70%), Postives = 250/465 (53.76%), Query Frame = 3
Query: 591 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGK-VMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDVVVVGFFKDQXXXXXXX-XXXXXXXKDDVVFGVTSQKAVFDEF---KVSSDDSIVLFK------KFDEGRNDFTGPFVDKDIRTFIDKYQLPIVIEFNQETAGKIFGGSIKKHVLSFISKKSEKYAE----LKGQFETSAKKFL--GQVLFVIINTDEEDNDRVLEFFGMAKTDTPSFRMIQLEDDMVKFKPDDATISSENLNLFVQSVLDGKVKPHMMTQEIPEDWDKNPVKVLVGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDSTANEVEG-VRIQSFPTIKYFPKDSDEMV-DFEGDRTLETFKKFIDS 1928
DV+E DS+F++ V K LVEF+APWCGHCK LAPEY AA LK+++ I +AKVD T E K + GYPTLK FK+G+ DY G R+AD II ++ K+GP + LK+ E S D VVVGFF ++S KA +D F T FDE K++ ++IV+F KF++ + + G + +RT++++ + + T + + + +++ + EK + + + AKKF+ G+ + I+ +E N + E+ G+ D + ++ KFK A S ENL F+ L+ KV ++ ++ IPE D+ PVKV+V KNFD + D +K V +EFYAPWCGHCK LAP +++L + +DIVIAKMD+TAN+V + FPTI + PK+S + R ++ K+I S
Sbjct: 18 DVLEFKDSSFEQSV-KEHSVILVEFYAPWCGHCKKLAPEYEIAATALKKQDPPIAIAKVDCTAETKTCEKHGVSGYPTLKVFKNGQFAFDYSGERSADGIIKYMVSKSGPSSKELKTEENFNKFTASADPVVVGFFTSESSDLYKAFLSVAEKLNEDFRFAHT-----FDETLSTKLNYKNNIVIFHSKKMANKFEDTQVKYKGDASEHQMRTWVEENIMGLA---GHRTHSNVKYFAKRPLIVALYNVDYEKNPKGTNYWRNRILKVAKKFIDSGKSIHFAISNVQEMNQELSEY-GIESPDFSKVYITARDERNQKFKMM-ADFSMENLEKFINDFLEEKVPVYLKSESIPEKNDE-PVKVVVAKNFDEIVNDPTKDVLIEFYAPWCGHCKSLAPKYEELAKKLSAESDIVIAKMDATANDVPSPYEVSGFPTIYFAPKNSKHSPKSYSSGREVDDMLKYIAS 470 HSP 2 Score: 89.7373 bits (221), Expect = 1.700e-18 Identity = 47/125 (37.60%), Postives = 72/125 (57.60%), Query Frame = 3
Query: 111 MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGV---VKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGERTAGSIV 476
M L + F LA + VL +S+F + ++++ +LVEFYAPWCGHC+ LAPE++ AATALK + + VD + + +G+ G+PT+K+F K+ DY GER+A I+
Sbjct: 1 MNILISLFFLLPLAMAS-------DVLEFKDSSFEQSVKEHSVILVEFYAPWCGHCKKLAPEYEIAATALKKQDPPIAIAKVDCTAETKTCEKHGVSGYPTLKVF-KNGQFAFDYSGERSADGII 117 HSP 3 Score: 84.7297 bits (208), Expect = 7.138e-17 Identity = 49/124 (39.52%), Postives = 68/124 (54.84%), Query Frame = 3
Query: 177 EEGVLVLTNSNFNEVI-EKNKYVLVEFYAPWCGHCQSLAPEWKKAATALKGVVKVGAVDMDKYSS-LGQPYGIQGFPTIKIFGK-SKHSPKDYQGERTA-------GSIVNAALKELDELVRSR 518
+E V V+ NF+E++ + K VL+EFYAPWCGHC+SLAP++++ A L + MD ++ + PY + GFPTI K SKHSPK Y R S ++ LK D R R
Sbjct: 363 DEPVKVVVAKNFDEIVNDPTKDVLIEFYAPWCGHCKSLAPKYEELAKKLSAESDIVIAKMDATANDVPSPYEVSGFPTIYFAPKNSKHSPKSYSSGREVDDMLKYIASEASSPLKNFDRQGRDR 486 The following BLAST results are available for this feature:
BLAST of Protein disulfide-isomerase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Protein disulfide-isomerase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Protein disulfide-isomerase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30017430 ID=SMED30017430|Name=Protein disulfide-isomerase|organism=Schmidtea mediterranea sexual|type=transcript|length=2357bpback to top protein sequence of SMED30017430-orf-1 >SMED30017430-orf-1 ID=SMED30017430-orf-1|Name=SMED30017430-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=641bp MKYLSIVLFCFALAQCNADITEEEGVLVLTNSNFNEVIEKNKYVLVEFYAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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