SMED30017324

Overview
NameSMED30017324
Smed IDSMED30017324
Length (bp)909
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30017324 (SMED30017324) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017324 (SMED30017324) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017324 (SMED30017324) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017324 (SMED30017324) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30017324

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30017324h1SMcG0001514 SmedASXL_004176SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30017324h1SMcG0001514 SmedASXL_014118SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30017324h1SMcG0001514 SMED_13717_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30017324h1SMcG0001514 SMED_13717_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30017324 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004246:13501..14492 +4480
v31.013252:12907..13898 +4444
Homology
BLAST of SMED30017324 vs. Planmine SMEST
Match: SMESG000032105.1 (SMESG000032105.1)

HSP 1 Score: 478.019 bits (1229), Expect = 2.076e-172
Identity = 256/257 (99.61%), Postives = 256/257 (99.61%), Query Frame = 1
Query:   49 MTNETPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELELNYSYEIFKGEVVYKNTITNVAYEIHPNYKIYRRIVNNYKDEQLAKISEALNTETFKNDKVNIVEHDTQSQINEIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDNLLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENYLYKDTDNLYQSKQFXXXXXXXXXXXXXLKVKCFNEKELDEFLEENLESYLSLKSIRK 819
            MTNETPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELELNYSYEIFKGEVVYKNTITNV YEIHPNYKIYRRIVNNYKDEQLAKISEALNTETFKNDKVNIVEHDTQSQINEIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDNLLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENYLYKDTDNLYQSKQFIINSDSEDEDDINLKVKCFNEKELDEFLEENLESYLSLKSIRK
Sbjct:    1 MTNETPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELELNYSYEIFKGEVVYKNTITNVVYEIHPNYKIYRRIVNNYKDEQLAKISEALNTETFKNDKVNIVEHDTQSQINEIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDNLLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENYLYKDTDNLYQSKQFIINSDSEDEDDINLKVKCFNEKELDEFLEENLESYLSLKSIRK 257          
BLAST of SMED30017324 vs. Planmine SMEST
Match: SMESG000050064.1 (SMESG000050064.1)

HSP 1 Score: 470.7 bits (1210), Expect = 1.265e-169
Identity = 252/257 (98.05%), Postives = 255/257 (99.22%), Query Frame = 1
Query:   49 MTNETPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELELNYSYEIFKGEVVYKNTITNVAYEIHPNYKIYRRIVNNYKDEQLAKISEALNTETFKNDKVNIVEHDTQSQINEIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDNLLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENYLYKDTDNLYQSKQFXXXXXXXXXXXXXLKVKCFNEKELDEFLEENLESYLSLKSIRK 819
            MTN TPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELEL YSYEIFKGEVVYKNTITNVAYEIHPNYKIYRRIVNNYKD+QLAKISEALNTETFKNDK+NIVEHDTQSQI+EIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDNLLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENYLYKDTDNLYQSKQFIINSDSEDEDDINLKVKCFNEKELDEFLEENLESYLSLKSIRK
Sbjct:    1 MTNGTPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELELKYSYEIFKGEVVYKNTITNVAYEIHPNYKIYRRIVNNYKDKQLAKISEALNTETFKNDKINIVEHDTQSQIDEIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDNLLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENYLYKDTDNLYQSKQFIINSDSEDEDDINLKVKCFNEKELDEFLEENLESYLSLKSIRK 257          
BLAST of SMED30017324 vs. Planmine SMEST
Match: SMESG000019256.1 (SMESG000019256.1)

HSP 1 Score: 282.722 bits (722), Expect = 1.957e-95
Identity = 170/257 (66.15%), Postives = 205/257 (79.77%), Query Frame = 1
Query:   49 MTNETPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELELNYSYEIFKGEVVYKNTITNVAYEIHPNYKIYRRIVNNYKDEQLAKISEALNTETFKNDKVNIVEHDTQSQINEIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDNLLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENYL--YKDTDNLYQSKQFXXXXXXXXXXXXXLKVKCFNEKELDEFLEENLESYLSLKSI 813
            MTN+  S++FQKYAKAIGVDIN ND  LL+IA  G+N+ELELNYSYEI+KGE+VYKN  TNVAYE+HPNYKIYR+IV+NYK EQ+  I EA N ET KN K+NIV+ DTQSQI+EIE   K L+LEY+ EV+L EHHVK+  GKFLNDDNL+KLSELT KFEEID   +E KTL K+ Q  LEKL D+ MKL+ +D EN     K+ ++L+Q +Q IINSDSEDEDDI+LKVKCFNEKE +E+L+  L S+LS KSI
Sbjct:    1 MTNKNRSKSFQKYAKAIGVDINENDNFLLSIANKGYNEELELNYSYEIYKGEIVYKNINTNVAYEVHPNYKIYRKIVSNYK-EQITNIFEASNNETTKNYKINIVKDDTQSQIDEIENKLKKLDLEYEIEVDLFEHHVKETHGKFLNDDNLIKLSELTKKFEEIDNFNQEYKTLVKITQLHLEKLYDKFMKLKPIDTENSSDKEKEENHLFQPEQCIINSDSEDEDDISLKVKCFNEKERNEYLDGILGSFLSSKSI 256          
BLAST of SMED30017324 vs. Planmine SMEST
Match: SMESG000019258.1 (SMESG000019258.1)

HSP 1 Score: 268.47 bits (685), Expect = 4.811e-90
Identity = 149/257 (57.98%), Postives = 188/257 (73.15%), Query Frame = 1
Query:   49 MTNETPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELELNYSYEIFKGEVVYKNTITNVAYEIHPNYKIYRRIVNNYKDEQLAKISEALNTETFKNDKVNIVEHDTQSQINEIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDNLLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENYLYKDTDNLYQSKQFXXXXXXXXXXXXXLKVKCFNEKELDEFLEENLESYLSLKSIRK 819
            MTN+ PS++FQKYAKAIGV+IN  D  L  IA SG+N+EL+L+YSYEI  GE+ Y+N  T+V YEIHPNYKIYR+IV+NYK++QL+KISE  N ET KNDK+NI+++D   QI+E EK  K  +L+YQTE+ELL+  V KAC +F NDDNLLKLS++   FEE+DKCKR  KTL K+ Q  LEK CD+I KLER  NEN  + D D++                  +LKVKCF+EKE+ EF++ENLES L LKSIRK
Sbjct:    1 MTNKNPSKSFQKYAKAIGVNINEKDEFLKYIADSGYNEELKLDYSYEIINGEIGYRNIDTSVTYEIHPNYKIYRKIVSNYKEQQLSKISEVSNAETSKNDKINILKND---QIDEFEKKLK--KLDYQTELELLKSRVNKACEQFSNDDNLLKLSDVIQIFEELDKCKRLSKTLVKITQVHLEKSCDEIKKLERSCNENPPFIDEDDI------------------DLKVKCFSEKEMSEFIDENLESCLPLKSIRK 234          
The following BLAST results are available for this feature:
BLAST of SMED30017324 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017324 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000032105.12.076e-17299.61SMESG000032105.1[more]
SMESG000050064.11.265e-16998.05SMESG000050064.1[more]
SMESG000019256.11.957e-9566.15SMESG000019256.1[more]
SMESG000019258.14.811e-9057.98SMESG000019258.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017324 ID=SMED30017324|Name=SMED30017324|organism=Schmidtea mediterranea sexual|type=transcript|length=909bp
AAATTGTTTAAATATTCAGTTTTATAATTATTTATTGTGCAGCCTAATAT
GACTAATGAAACTCCAAGTCAAGCTTTTCAAAAATATGCTAAAGCTATTG
GTGTAGATATTAACAGCAATGACATAGTTTTACTAAATATTGCATACAGC
GGTTTCAATAAAGAATTAGAATTAAACTATTCATATGAGATATTCAAAGG
CGAGGTTGTGTATAAAAATACCATTACAAATGTTGCTTATGAAATTCATC
CAAATTACAAAATTTATCGTAGAATTGTCAATAATTACAAAGATGAACAA
CTTGCTAAAATATCTGAAGCTTTAAATACTGAAACATTCAAAAATGATAA
AGTTAACATTGTAGAACATGACACCCAATCTCAAATTAATGAAATTGAGA
AAAATTTTAAAAATTTGGAGTTGGAATATCAAACAGAAGTTGAATTGCTC
GAACATCATGTCAAGAAAGCATGTGGAAAATTCTTAAACGATGATAATTT
ATTAAAATTATCAGAACTGACACACAAATTTGAAGAGATTGACAAATGCA
AGCGGGAGTGTAAAACTTTGTATAAAGTTTGTCAAGCCGAACTCGAGAAA
TTATGCGATCAAATTATGAAATTAGAACGATTAGACAATGAAAATTACTT
GTATAAAGATACGGATAATTTGTATCAGTCTAAGCAGTTTATTATTAATT
CGGACTCGGAAGATGAAGACGACATCAATCTCAAAGTCAAATGTTTTAAC
GAGAAAGAACTAGACGAATTCCTTGAGGAAAACCTAGAAAGCTATTTGTC
ACTTAAATCCATCCGGAAATGAGAGAGGAAATTTCTGTCGGTTGTTTTGC
GTGTTCTTTTGTACAGTTTTATCCGTATTTAAATAGATTTTATATCGGTT
TTATTTAAA
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protein sequence of SMED30017324-orf-1

>SMED30017324-orf-1 ID=SMED30017324-orf-1|Name=SMED30017324-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=258bp
MTNETPSQAFQKYAKAIGVDINSNDIVLLNIAYSGFNKELELNYSYEIFK
GEVVYKNTITNVAYEIHPNYKIYRRIVNNYKDEQLAKISEALNTETFKND
KVNIVEHDTQSQINEIEKNFKNLELEYQTEVELLEHHVKKACGKFLNDDN
LLKLSELTHKFEEIDKCKRECKTLYKVCQAELEKLCDQIMKLERLDNENY
LYKDTDNLYQSKQFIINSDSEDEDDINLKVKCFNEKELDEFLEENLESYL
SLKSIRK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 175..195
NoneNo IPR availableCOILSCoilCoilcoord: 106..133