SMED30017235
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30017235 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 18
Homology
BLAST of SMED30017235 vs. Planmine SMEST
Match: SMESG000079668.1 (SMESG000079668.1) HSP 1 Score: 362.844 bits (930), Expect = 5.920e-123 Identity = 196/288 (68.06%), Postives = 211/288 (73.26%), Query Frame = 2 Query: 737 MSKGFRNKNSMNEDPANGYAYHVTSTDCCGRTDNDMLQINWIRNRVD------------------ECLDHNSSILSGESQPNQQEEDEKDLTYPFKMKTEINCELKAFRIRESYYHPTVEGVEGVFMSTTLYSNQKDQKSVSKDIPLYLPPTTVYPSG------DHYYQRISIKFDEISAKYPKCKFWCEKTFKNTTQRHYICYNSDMEIEKFKKPNSASCGIKFTEDIED--WNELGIEKEGGETKDILPRLQNDYNLKKYFNNIVLLGDIKIPEAPGDRKIDIVKK 1522 MSKGFRNKNSMNEDPANGYAYHVTSTDCCGRTDNDMLQINWIRNRVD ECLDHNSSILSGESQPNQQEEDEKDLTYPFKMKTEINCELKAFRIRESYYHPTVEGVEGVFMSTTLY +LP TVYP G DHYYQRISIKFD+IS KYP+CKFWC K QRHYICY S+ +I+ F K SA+ GIKF+ED +D +GI+KEG TKDILP LQNDY ++YFNN+VLLG I+I GD IDI K Sbjct: 1 MSKGFRNKNSMNEDPANGYAYHVTSTDCCGRTDNDMLQINWIRNRVDGKKIFVFIKFFYILFGVKECLDHNSSILSGESQPNQQEEDEKDLTYPFKMKTEINCELKAFRIRESYYHPTVEGVEGVFMSTTLYDG-------------HLPDWTVYPFGKPQCQVDHYYQRISIKFDKISEKYPQCKFWCIK----DKQRHYICYKSESKIKNFNKTKSATAGIKFSEDFQDSELEVMGIKKEGA-TKDILPYLQNDYKKEEYFNNLVLLGGIEI---LGDAIIDIAHK 267
BLAST of SMED30017235 vs. Planmine SMEST
Match: SMESG000079382.1 (SMESG000079382.1) HSP 1 Score: 254.218 bits (648), Expect = 8.913e-81 Identity = 153/284 (53.87%), Postives = 182/284 (64.08%), Query Frame = 2 Query: 710 YHCIKDSEEMSKGFRNKNSMNEDPANGYAYHVTSTDCCGRTDNDMLQINWIRNRVDECLDHNSSILSGES-QPNQQEEDEKDLTYPFKMKTEIN----CELKAFRIRESYYHPTVEGVEGVFMSTTLYSNQKDQKSVSKDIPLYLPPTTVYPSG------DHYYQRISIKFDEISAKYPKCKFWCEKTFKNTTQRHYICYNSDMEIEKFKKPNSASCGIKFTEDIED--WNELGIEKEGGETKDILPRLQNDYNLKKYFNNIVLLGDIKIPEAPGDRKIDIVKK 1522 YHCIKD + FRNK DPANGYAYHVT+ DCCGRT +M +INWIRNRVDE ++ SILSGES + +++D+T IN EL+ F IR+S YHP VEGVEGVFM+TTLY +LP TVYP G DHYYQRISIKFD+IS KYP+CKFWC K QRHYICY S+ +I+ F K SA+ GIKF+ED +D +GI+KEG TKDILP LQNDY ++YFNN+VLLG I+I GD IDI K Sbjct: 5 YHCIKDKN--MEDFRNK-----DPANGYAYHVTNIDCCGRTKLNMKKINWIRNRVDERVESIKSILSGESQPNQPNQPNQQDMTGLADQLAAINLNEDLELRPFEIRKSNYHPAVEGVEGVFMNTTLYDG-------------HLPDWTVYPFGKPQCQVDHYYQRISIKFDKISEKYPQCKFWCIK----DKQRHYICYKSESKIKNFNKTKSATAGIKFSEDFQDSELEVMGIKKEGA-TKDILPYLQNDYKKEEYFNNLVLLGGIEI---LGDAIIDIAHK 260
BLAST of SMED30017235 vs. Planmine SMEST
Match: SMESG000079382.1 (SMESG000079382.1) HSP 1 Score: 246.899 bits (629), Expect = 4.946e-78 Identity = 147/271 (54.24%), Postives = 175/271 (64.58%), Query Frame = 2 Query: 749 FRNKNSMNEDPANGYAYHVTSTDCCGRTDNDMLQINWIRNRVDECLDHNSSILSGES-QPNQQEEDEKDLTYPFKMKTEIN----CELKAFRIRESYYHPTVEGVEGVFMSTTLYSNQKDQKSVSKDIPLYLPPTTVYPSG------DHYYQRISIKFDEISAKYPKCKFWCEKTFKNTTQRHYICYNSDMEIEKFKKPNSASCGIKFTEDIED--WNELGIEKEGGETKDILPRLQNDYNLKKYFNNIVLLGDIKIPEAPGDRKIDIVKK 1522 FRNK DPANGYAYHVT+ DCCGRT +M +INWIRNRVDE ++ SILSGES + +++D+T IN EL+ F IR+S YHP VEGVEGVFM+TTLY +LP TVYP G DHYYQRISIKFD+IS KYP+CKFWC K QRHYICY S+ +I+ F K SA+ GIKF+ED +D +GI+KEG TKDILP LQNDY ++YFNN+VLLG I+I GD IDI K Sbjct: 4 FRNK-----DPANGYAYHVTNIDCCGRTKLNMKKINWIRNRVDERVESIKSILSGESQPNQPNQPNQQDMTGLADQLAAINLNEDLELRPFEIRKSNYHPAVEGVEGVFMNTTLYDG-------------HLPDWTVYPFGKPQCQVDHYYQRISIKFDKISEKYPQCKFWCIK----DKQRHYICYKSESKIKNFNKTKSATAGIKFSEDFQDSELEVMGIKKEGA-TKDILPYLQNDYKKEEYFNNLVLLGGIEI---LGDAIIDIAHK 248
BLAST of SMED30017235 vs. Planmine SMEST
Match: SMESG000079644.1 (SMESG000079644.1) HSP 1 Score: 140.584 bits (353), Expect = 1.252e-37 Identity = 111/294 (37.76%), Postives = 152/294 (51.70%), Query Frame = 2 Query: 737 MSKGFRNKNSMNEDPANGYAYHVTSTDCCGRTDNDMLQINWIRNRVDECLDHNSSILSGESQPNQQEEDEKDLTYPFKMKTEINCE---LKAFRIRESYYHPTVEGVEGVFMSTTLYSNQ---------KDQKSVSKDIPLYLPPTTV--YPSGDHYYQRISIKFDEISAKYPKCKFWC---------EKTFKNTT---QRHYICYNS----------------DMEIEKFKKPNSASCGIKFTEDIEDWN--ELGIEKEGGETKDILPRLQNDYNLKKYFNNIVLLGDIKIPE 1486 M+ FR +SM GYAYHVTS DCCG+TDN M I+W N + + + + ESQ + +E +DL K I LKAF I++ YHP+ EGV+ VF++ TL++N ++ S+D Y P+ YP G H+YQRISIKF IS +YPKCKFWC + F N++ Q YICYN D+EI + + S I F +DI+ + +L I K+G TK+IL +QN+Y Y+ +IVLLGDI I E Sbjct: 1 MNSLFRTTHSMR-----GYAYHVTSIDCCGKTDNTMRIIDWDHNPPRDGFHY---LDAEESQTSNEENSVEDLIDQMGKKVHIKDNKNILKAFEIKKCKYHPSAEGVKAVFLNLTLFNNSLPNSTKVYPHARQIFSED---YCIPSRRLEYPYG-HFYQRISIKFKRISKRYPKCKFWCLMKDEDKGRSEKFTNSSCVKQISYICYNEEEEIENITFENKYEEYDVEIVR-----NRSDEILFYDDIDQYERCKLKIIKDGA-TKNILNLIQNNYYKNTYYTSIVLLGDIDISE 276
BLAST of SMED30017235 vs. Planmine SMEST
Match: SMESG000079644.1 (SMESG000079644.1) HSP 1 Score: 137.117 bits (344), Expect = 2.021e-36 Identity = 106/277 (38.27%), Postives = 145/277 (52.35%), Query Frame = 2 Query: 788 GYAYHVTSTDCCGRTDNDMLQINWIRNRVDECLDHNSSILSGESQPNQQEEDEKDLTYPFKMKTEINCE---LKAFRIRESYYHPTVEGVEGVFMSTTLYSNQ---------KDQKSVSKDIPLYLPPTTV--YPSGDHYYQRISIKFDEISAKYPKCKFWC---------EKTFKNTT---QRHYICYNS----------------DMEIEKFKKPNSASCGIKFTEDIEDWN--ELGIEKEGGETKDILPRLQNDYNLKKYFNNIVLLGDIKIPE 1486 GYAYHVTS DCCG+TDN M I+W N + + + + ESQ + +E +DL K I LKAF I++ YHP+ EGV+ VF++ TL++N ++ S+D Y P+ YP G H+YQRISIKF IS +YPKCKFWC + F N++ Q YICYN D+EI + + S I F +DI+ + +L I K+G TK+IL +QN+Y Y+ +IVLLGDI I E Sbjct: 3 GYAYHVTSIDCCGKTDNTMRIIDWDHNPPRDGFHY---LDAEESQTSNEENSVEDLIDQMGKKVHIKDNKNILKAFEIKKCKYHPSAEGVKAVFLNLTLFNNSLPNSTKVYPHARQIFSED---YCIPSRRLEYPYG-HFYQRISIKFKRISKRYPKCKFWCLMKDEDKGRSEKFTNSSCVKQISYICYNEEEEIENITFENKYEEYDVEIVR-----NRSDEILFYDDIDQYERCKLKIIKDGA-TKNILNLIQNNYYKNTYYTSIVLLGDIDISE 266 The following BLAST results are available for this feature:
BLAST of SMED30017235 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30017235 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30017235 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30017235 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30017235 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30017235 ID=SMED30017235|Name=SMED30017235|organism=Schmidtea mediterranea sexual|type=transcript|length=1680bpback to top protein sequence of SMED30017235-orf-1 >SMED30017235-orf-1 ID=SMED30017235-orf-1|Name=SMED30017235-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=273bp MSKGFRNKNSMNEDPANGYAYHVTSTDCCGRTDNDMLQINWIRNRVDECLback to top Annotated Terms
The following terms have been associated with this transcript:
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