ANK_REP_REGION domain-containing protein

Overview
NameANK_REP_REGION domain-containing protein
Smed IDSMED30017228
Length (bp)446
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017228

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30017228SMESG000039339.1 dd_Smed_v4_35336_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30017228SMESG000039339.1 dd_Smed_v4_35336_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30017228SMESG000039339.1 dd_Smed_v4_35336_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30017228SMESG000039339.1 dd_Smed_v6_35336_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Celegans
Match: ocr-4 (Osm-9 and Capsaicin receptor-Related [Source:UniProtKB/TrEMBL;Acc:Q9N3Y9])

HSP 1 Score: 56.225 bits (134), Expect = 9.279e-10
Identity = 29/83 (34.94%), Postives = 50/83 (60.24%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVK--VQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYR 293
            MGN +S V   +K  V A N A++  K  D+SG G   ++     +T  ++++DK+I E + P LYNNG+G+++ + +L+  R
Sbjct:    1 MGNASSAVTAGIKNQVNASNNAIN--KLADMSGRGFLADLAMDAVRTGNHEELDKQILEKVQPMLYNNGQGEMIPLSDLIAQR 81
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Celegans
Match: ocr-1 (Osm-9 and Capsaicin receptor-Related [Source:UniProtKB/TrEMBL;Acc:G5EDR9])

HSP 1 Score: 45.8246 bits (107), Expect = 3.315e-6
Identity = 24/89 (26.97%), Postives = 46/89 (51.69%), Query Frame = 3 
Query:   51 MGNVNSNVHNAV--------KVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYR 293
            MG  NS + +          +  ++NK +  +K +D+ G G   ++ + C +   +  +D  + + +  Y+YN G+GKL+ V ELV+ R
Sbjct:    1 MGTKNSKIADGAAQVEQTMARTMSENKVI--YKMVDMHGGGILIKLARKCKELNSFVLLDAYLDQEVRKYMYNGGKGKLMPVAELVKAR 87
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Fly
Match: nan (gene:FBgn0036414 transcript:FBtr0089524)

HSP 1 Score: 63.929 bits (154), Expect = 2.489e-12
Identity = 44/143 (30.77%), Postives = 68/143 (47.55%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRXXXXXXXXXXXXXXXXXXXXIYPELQIIR---EDENGRENPP-------IINPL-YREVCWD 446
            MGN  SNV + VK QA     + +K++++ G G   +MMK   +TKQ+ ++D  I   + P+LYN G G+   + +LV  R+       +             PE++ +    +D N  +  P       I NP  YR VCWD
Sbjct:    1 MGNTESNVTSGVKKQAGVSTQALYKFVNLKGGGLLVDMMKRACQTKQFAEIDHAIKTKVEPFLYNKGAGRYFPISKLVLLRNRDRPRTRQ------------LPEIRALENPDDDFNIHDYCPEVSEAEYISNPTAYRFVCWD 131
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Fly
Match: nan (gene:FBgn0036414 transcript:FBtr0321258)

HSP 1 Score: 63.929 bits (154), Expect = 2.513e-12
Identity = 44/143 (30.77%), Postives = 68/143 (47.55%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRXXXXXXXXXXXXXXXXXXXXIYPELQIIR---EDENGRENPP-------IINPL-YREVCWD 446
            MGN  SNV + VK QA     + +K++++ G G   +MMK   +TKQ+ ++D  I   + P+LYN G G+   + +LV  R+       +             PE++ +    +D N  +  P       I NP  YR VCWD
Sbjct:    1 MGNTESNVTSGVKKQAGVSTQALYKFVNLKGGGLLVDMMKRACQTKQFAEIDHAIKTKVEPFLYNKGAGRYFPISKLVLLRNRDRPRTRQ------------LPEIRALENPDDDFNIHDYCPEVSEAEYISNPTAYRFVCWD 131
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BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: A0A1I8HBT4 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.684e-17
Identity = 51/150 (34.00%), Postives = 75/150 (50.00%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRXX------XXXXXXXXXXXXXXXXXXIYPELQIIREDENG------------RENPPIINPLYREVCWD 446
            MGN   N+ ++VK Q DNKA   ++++D++G GE  E  K C   + Y  +D++I   +  +LYNNGEGK V V ELV+YR+         +   +         N +YP L I+  D +               NP + N  YR+VCWD
Sbjct:    1 MGNNQPNIQSSVKEQTDNKAFKAYRFVDLTGGGELIEAAKKCQTAEDYRLLDERIKHEMGLFLYNNGEGKKVHVSELVKYRTRDGNPPLAKSPAGRHGRRSTPTGNNVYPMLDIVHADTDATGTCVSGGGGGGGTNPEVDNG-YRDVCWD 149
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BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: A0A1S3IFS8 (transient receptor potential cation channel subfamily V member 5 OS=Lingula unguis OX=7574 GN=LOC106163936 PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.769e-15
Identity = 36/82 (43.90%), Postives = 61/82 (74.39%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKI-YEYLSPYLYNNGEGKLVSVDELVRYR 293
            MGN+N+NV + +K Q D+K+ S +KY+D++G GE  E+ K  N++KQ+D++D+KI  + +  +LYN G+G LV +++L+ YR
Sbjct:    1 MGNINTNVTSGMKAQGDSKSKSLYKYVDLNGGGELVELQKEANRSKQFDKIDEKIRSDEIKGFLYNGGDGMLVDINKLIEYR 82

HSP 2 Score: 58.9214 bits (141), Expect = 3.893e-7
Identity = 27/71 (38.03%), Postives = 48/71 (67.61%), Query Frame = 3 
Query:   84 VKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKI-YEYLSPYLYNNGEGKLVSVDELVRYR 293
            V+V  +      +KY+D++G GE  E+ K  N++KQ+D++D+KI  + +  +LYN G+G LV +++L+ YR
Sbjct:  263 VEVSQETNYDGLYKYVDLNGGGELVELQKEANRSKQFDKIDEKIRSDEIKGFLYNGGDGMLVDINKLIEYR 333
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BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: A0A4Y2CAA5 (Transient receptor potential cation channel subfamily V member 5 OS=Araneus ventricosus OX=182803 GN=Trpv5 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.779e-15
Identity = 47/132 (35.61%), Postives = 71/132 (53.79%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRXXXXXXXXXXXXXXXXXXXXIYPELQIIREDENGRENPPIINPLYREVCWD 446
            MGN  SNV + VK Q D+ ++  +  +DV G G+   +MK   KT+ Y  +D +I E + P+LYNNGEGK+V + ELV  R+    +  +    ++    +  P+   I  D   +E      P +REVCWD
Sbjct:    1 MGNAESNVTSGVKKQTDSGSLKIYSLVDVKGGGQIVTLMKEAAKTRDYTALDNQIRELVLPFLYNNGEGKMVPIQELVLTRNKDRPKHKQLPQNRDPDAKST-PKSSSINIDNIAKEED---GPQFREVCWD 128
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BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: T1IIR8 (ANK_REP_REGION domain-containing protein OS=Strigamia maritima OX=126957 PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.406e-13
Identity = 49/132 (37.12%), Postives = 74/132 (56.06%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQY-DQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRXXXXXXXXXXXXXXXXXXXXIYPELQIIREDENGRENPPIINPLYREVCW 443
            MGN +SNV + VK QAD  ++  +K ++++G GE  + MK   K K+  +++++KIYE + P+LYNNGEGKL+ V ELV  R         K+  +  +   + PE      D NG  +  I    +R VCW
Sbjct:    1 MGNTSSNVTSGVKKQADAGSLEIYKLINLAGGGELVDFMKNATKNKKSSEEIEEKIYELVLPFLYNNGEGKLMPVSELVLMR--------NKECGREKQLQGLRPEELSNSTDLNGATSKKIDMNKHRLVCW 124
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BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Match: A0A2D3C346 (TRP channel protein nanchung OS=Nilaparvata lugens OX=108931 GN=nan PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.444e-13
Identity = 46/143 (32.17%), Postives = 70/143 (48.95%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRXXXXXXXXXXXXXXXXXXXXIYPELQIIREDEN----GRENPPIINP-------LYREVCWD 446
            MGN  SNV + VK Q D  +++ +K +D+ G G   E+MK   + KQY ++D  I   + P+LYN G G+L+S+ +LV  R             K+     + P L+ +   ++    G   P I  P        YR+VCWD
Sbjct:    1 MGNTESNVTSGVKKQTDTSSITMYKLVDLKGGGLLVELMKRATQNKQYAELDHAIKTKVEPFLYNKGNGRLISIAKLVLLR------------NKDRPRTKMLPPLKNMENPDDFDVIGSNIPGIPEPKETDDPSTYRDVCWD 131
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BLAST of ANK_REP_REGION domain-containing protein vs. Planmine SMEST
Match: SMESG000039339.1 (SMESG000039339.1)

HSP 1 Score: 231.106 bits (588), Expect = 1.131e-72
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 3 
Query:   51 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRXXXXXXXXXXXXXXXXXXXXIYPELQIIREDENGRENPPIINPLYREVCWD 446
            MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRSGKSTEKDKKKNTKNSKHNTIYPELQIIREDENGRENPPIINPLYREVCWD
Sbjct:    1 MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQMDKKIYEYLSPYLYNNGEGKLVSVDELVRYRSGKSTEKDKKKNTKNSKHNTIYPELQIIREDENGRENPPIINPLYREVCWD 132
The following BLAST results are available for this feature:
BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ocr-49.279e-1034.94Osm-9 and Capsaicin receptor-Related [Source:UniP... [more]
ocr-13.315e-626.97Osm-9 and Capsaicin receptor-Related [Source:UniP... [more]
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nan2.489e-1230.77gene:FBgn0036414 transcript:FBtr0089524[more]
nan2.513e-1230.77gene:FBgn0036414 transcript:FBtr0321258[more]
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8HBT41.684e-1734.00ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A1S3IFS82.769e-1543.90transient receptor potential cation channel subfam... [more]
A0A4Y2CAA52.779e-1535.61Transient receptor potential cation channel subfam... [more]
T1IIR81.406e-1337.12ANK_REP_REGION domain-containing protein OS=Striga... [more]
A0A2D3C3462.444e-1332.17TRP channel protein nanchung OS=Nilaparvata lugens... [more]
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ANK_REP_REGION domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000039339.11.131e-72100.00SMESG000039339.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017228 ID=SMED30017228|Name=ANK_REP_REGION domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=446bp
ATTTTACTTATTACATTTCAAACCGGAATTACTTTTTTCTTAAACACTAA
ATGGGAAACGTCAACTCTAATGTCCATAACGCTGTTAAGGTTCAAGCCGA
TAATAAAGCAGTCAGTTGGTTCAAATACTTGGATGTATCGGGGGCAGGCG
AATTTTTCGAGATGATGAAAATATGCAACAAAACTAAGCAGTATGATCAG
ATGGATAAGAAGATTTATGAATATTTATCACCTTACTTATATAACAATGG
AGAGGGTAAACTTGTATCTGTTGATGAATTAGTTAGATATCGAAGTGGGA
AATCAACGGAGAAAGATAAAAAGAAAAATACAAAAAACTCCAAACACAAT
ACCATATATCCAGAATTACAAATTATACGTGAAGACGAAAATGGTAGAGA
AAATCCTCCAATAATCAACCCACTTTATCGTGAGGTTTGTTGGGAC
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protein sequence of SMED30017228-orf-1

>SMED30017228-orf-1 ID=SMED30017228-orf-1|Name=SMED30017228-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=132bp
MGNVNSNVHNAVKVQADNKAVSWFKYLDVSGAGEFFEMMKICNKTKQYDQ
MDKKIYEYLSPYLYNNGEGKLVSVDELVRYRSGKSTEKDKKKNTKNSKHN
TIYPELQIIREDENGRENPPIINPLYREVCWD
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR024862TRPV
Vocabulary: molecular function
TermDefinition
GO:0005216ion channel activity
Vocabulary: biological process
TermDefinition
GO:0006811ion transport
GO:0034220ion transmembrane transport
GO:0055085transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..98
NoneNo IPR availablePANTHERPTHR10582:SF28NANCHUNG, ISOFORM Bcoord: 1..132
IPR024862Transient receptor potential cation channel subfamily VPANTHERPTHR10582TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEINcoord: 1..132