spermatogenesis-associated serine-rich protein 1-like

Overview
Namespermatogenesis-associated serine-rich protein 1-like
Smed IDSMED30017224
Length (bp)874
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of spermatogenesis-associated serine-rich protein 1-like (SMED30017224) t-SNE clustered cells

Violin plots show distribution of expression levels for spermatogenesis-associated serine-rich protein 1-like (SMED30017224) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of spermatogenesis-associated serine-rich protein 1-like (SMED30017224) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for spermatogenesis-associated serine-rich protein 1-like (SMED30017224) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017224

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30017224SMESG000043323.1 SmedASXL_006894SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30017224SMESG000043323.1 dd_Smed_v4_8305_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30017224SMESG000043323.1 dd_Smed_v4_8305_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30017224SMESG000043323.1 dd_Smed_v4_8305_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30017224SMESG000043323.1 dd_Smed_v4_8305_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30017224SMESG000043323.1 dd_Smed_v6_8305_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30017224SMESG000043323.1 dd_Smed_v4_8305_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Human
Match: SPATS1 (spermatogenesis associated serine rich 1 [Source:HGNC Symbol;Acc:HGNC:22957])

HSP 1 Score: 63.929 bits (154), Expect = 3.777e-11
Identity = 45/131 (34.35%), Postives = 65/131 (49.62%), Query Frame = 1 
Query:  349 GKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRIS-RAKHDTFIPI--FPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISVLY 732
            GK+C F    L +K S  E T    FG  RK  +ID RNG   L+ GD  Y   E S  F+K G  +P V F  +    K DTFIP+   P +    + +K      K ++  V++LD W+P+   + +L+
Sbjct:  161 GKQCFFNGVFLGNKRSLSERTVDKCFG--RKKYDIDPRNGIPKLTPGDNPYMYPEQSKGFHKAGSMLPPVNFSIVPYEKKFDTFIPLEPLPQIPNLPFWVKEKANSLKNEIQEVEELDNWQPAVPLMHMLH 289
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Human
Match: SPATS1 (spermatogenesis associated serine rich 1 [Source:HGNC Symbol;Acc:HGNC:22957])

HSP 1 Score: 63.929 bits (154), Expect = 3.777e-11
Identity = 45/131 (34.35%), Postives = 65/131 (49.62%), Query Frame = 1 
Query:  349 GKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRIS-RAKHDTFIPI--FPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISVLY 732
            GK+C F    L +K S  E T    FG  RK  +ID RNG   L+ GD  Y   E S  F+K G  +P V F  +    K DTFIP+   P +    + +K      K ++  V++LD W+P+   + +L+
Sbjct:  161 GKQCFFNGVFLGNKRSLSERTVDKCFG--RKKYDIDPRNGIPKLTPGDNPYMYPEQSKGFHKAGSMLPPVNFSIVPYEKKFDTFIPLEPLPQIPNLPFWVKEKANSLKNEIQEVEELDNWQPAVPLMHMLH 289
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Human
Match: SPATS1 (spermatogenesis associated serine rich 1 [Source:HGNC Symbol;Acc:HGNC:22957])

HSP 1 Score: 63.929 bits (154), Expect = 3.777e-11
Identity = 45/131 (34.35%), Postives = 65/131 (49.62%), Query Frame = 1 
Query:  349 GKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRIS-RAKHDTFIPI--FPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISVLY 732
            GK+C F    L +K S  E T    FG  RK  +ID RNG   L+ GD  Y   E S  F+K G  +P V F  +    K DTFIP+   P +    + +K      K ++  V++LD W+P+   + +L+
Sbjct:  161 GKQCFFNGVFLGNKRSLSERTVDKCFG--RKKYDIDPRNGIPKLTPGDNPYMYPEQSKGFHKAGSMLPPVNFSIVPYEKKFDTFIPLEPLPQIPNLPFWVKEKANSLKNEIQEVEELDNWQPAVPLMHMLH 289
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Zebrafish
Match: BX255920.1 (spermatogenesis associated serine rich 1 [Source:NCBI gene;Acc:103909334])

HSP 1 Score: 52.7582 bits (125), Expect = 8.380e-8
Identity = 52/208 (25.00%), Postives = 92/208 (44.23%), Query Frame = 1 
Query:  124 DKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESL----WPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDK----GKKCIFTEG--HLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYK-NGVTVPVVPFGRISRAKHDTFIPIFPYV--KGSDYNLKRNDLMKKEDVLNVKDLDCWEPS 708
            ++ + PH   IEP  T    DW+S +R+IP P+  +     +    +P +   +R++P     +   W+  + N+ Q K    GK+C + +G  H +S     E + +S  G K+++ +       N  S   + +   EYSPDF+K          +G        + I + P    K   Y       +++ED+L VK L+ W P+
Sbjct:   18 ERDFKPHITHIEPVLTECDLDWKSHMRWIPEPRYSDAPFPHIKDIKFPSERKLMRSFPQANMVSGSEWS-FYPNLGQPKTYHNGKRC-YMDGINHTRSTRCISENSLESSIGKKKQVTD----KYSNYPSRKVRPFLVPEYSPDFHKCESAAWCTTNYGSSYCGALKSLIHLKPPTKYKRPPYAENHKGRVREEDILEVKSLNSWRPA 219
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Xenopus
Match: hmcn2 (hemicentin 2 [Source:Xenbase;Acc:XB-GENE-6034990])

HSP 1 Score: 83.9593 bits (206), Expect = 2.309e-18
Identity = 63/205 (30.73%), Postives = 100/205 (48.78%), Query Frame = 1 
Query:  136 IPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGK----NESLWPEKSSHIRTYPSL--LYSNYFVWNPKFENIRQDKGKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPI--FPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISV 726
            +P  N   P+Y NS  DW+ S R++PSP+  +       +  +P++   +R  PS   + S +  +           GK+C F   HL ++ S D     + FG K+K++  D RNG    + GDK + + EYSP F+K G T P++ F    R + DTFIP+   P      Y  K      +E    VK+L  W+PS+ + ++
Sbjct:   42 LPSINYPVPSYPNSDLDWKPSARWLPSPRYSDAPFPHIKDMKFPDRIRLMRQGPSPGDMGSEWSFYPTSGLPFTYHLGKRCAFEGRHLGNRASLDVQQLDTCFGKKKKVR--DPRNGIPFSNPGDKPFHSPEYSPSFHKFGSTRPLISFRDSYRVRADTFIPLQKMPPNPRVPYREKEAKQNWEEAKQEVKELSTWKPSQRSFTL 244
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Mouse
Match: Spats1 (spermatogenesis associated, serine-rich 1 [Source:MGI Symbol;Acc:MGI:1918270])

HSP 1 Score: 56.9954 bits (136), Expect = 5.246e-9
Identity = 43/123 (34.96%), Postives = 61/123 (49.59%), Query Frame = 1 
Query:  349 GKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRIS-RAKHDTFIPIFPYVKGSDYNL----KRNDLMKKEDVLNVKDLDCWE 702
            GK+C F      ++ S  E T  +  G K+   +ID RNG   L+ GD  Y   E S +F+K G T+P V F       K DTFIP+ P  K  +       K N+L  K ++  V++LD W+
Sbjct:  130 GKQCFFNGVFRGNRRSVAERTVDNSLGKKK--YDIDPRNGIPKLTPGDNPYMFPEQSKEFFKAGATLPPVNFSLGPYEKKFDTFIPLEPLPKIPNLPFWEKEKANNL--KNEIKEVEELDNWQ 248
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Mouse
Match: Spats1 (spermatogenesis associated, serine-rich 1 [Source:MGI Symbol;Acc:MGI:1918270])

HSP 1 Score: 56.6102 bits (135), Expect = 8.725e-9
Identity = 43/123 (34.96%), Postives = 61/123 (49.59%), Query Frame = 1 
Query:  349 GKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRIS-RAKHDTFIPIFPYVKGSDYNL----KRNDLMKKEDVLNVKDLDCWE 702
            GK+C F      ++ S  E T  +  G K+   +ID RNG   L+ GD  Y   E S +F+K G T+P V F       K DTFIP+ P  K  +       K N+L  K ++  V++LD W+
Sbjct:  130 GKQCFFNGVFRGNRRSVAERTVDNSLGKKK--YDIDPRNGIPKLTPGDNPYMFPEQSKEFFKAGATLPPVNFSLGPYEKKFDTFIPLEPLPKIPNLPFWEKEKANNL--KNEIKEVEELDNWQ 248
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. UniProt/SwissProt
Match: sp|Q496A3|SPAS1_HUMAN (Spermatogenesis-associated serine-rich protein 1 OS=Homo sapiens OX=9606 GN=SPATS1 PE=1 SV=2)

HSP 1 Score: 63.929 bits (154), Expect = 1.814e-10
Identity = 45/131 (34.35%), Postives = 65/131 (49.62%), Query Frame = 1 
Query:  349 GKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRIS-RAKHDTFIPI--FPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISVLY 732
            GK+C F    L +K S  E T    FG  RK  +ID RNG   L+ GD  Y   E S  F+K G  +P V F  +    K DTFIP+   P +    + +K      K ++  V++LD W+P+   + +L+
Sbjct:  161 GKQCFFNGVFLGNKRSLSERTVDKCFG--RKKYDIDPRNGIPKLTPGDNPYMYPEQSKGFHKAGSMLPPVNFSIVPYEKKFDTFIPLEPLPQIPNLPFWVKEKANSLKNEIQEVEELDNWQPAVPLMHMLH 289
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. UniProt/SwissProt
Match: sp|A2RRY8|SPAS1_MOUSE (Spermatogenesis-associated serine-rich protein 1 OS=Mus musculus OX=10090 GN=Spats1 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.669e-8
Identity = 43/123 (34.96%), Postives = 61/123 (49.59%), Query Frame = 1 
Query:  349 GKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRIS-RAKHDTFIPIFPYVKGSDYNL----KRNDLMKKEDVLNVKDLDCWE 702
            GK+C F      ++ S  E T  +  G K+   +ID RNG   L+ GD  Y   E S +F+K G T+P V F       K DTFIP+ P  K  +       K N+L  K ++  V++LD W+
Sbjct:  130 GKQCFFNGVFRGNRRSVAERTVDNSLGKKK--YDIDPRNGIPKLTPGDNPYMFPEQSKEFFKAGATLPPVNFSLGPYEKKFDTFIPLEPLPKIPNLPFWEKEKANNL--KNEIKEVEELDNWQ 248
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. UniProt/SwissProt
Match: sp|Q811V6|SPAS1_RAT (Spermatogenesis-associated serine-rich protein 1 OS=Rattus norvegicus OX=10116 GN=Spats1 PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 8.448e-8
Identity = 43/123 (34.96%), Postives = 60/123 (48.78%), Query Frame = 1 
Query:  349 GKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRIS-RAKHDTFIPIFPYVKGSDYNL----KRNDLMKKEDVLNVKDLDCWE 702
            GK+C F      ++ S  E T     G K+   +ID RNG   L+ GD  Y   E S +F+K G T+P V F       K DTFIP+ P  +  +       K N+L  K ++  V+DLD W+
Sbjct:  139 GKQCFFNGVFRGNRKSVAERTVDKSLGKKK--YDIDPRNGIPKLTPGDNPYMFPEQSKEFFKAGATLPPVNFSLGPYEKKFDTFIPLEPLPQIPNLPFWEKEKANNL--KNEIKEVEDLDNWQ 257
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. TrEMBL
Match: A0A1I8FV88 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 1.222e-63
Identity = 104/213 (48.83%), Postives = 136/213 (63.85%), Query Frame = 1 
Query:   82 RRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDKGKKCIFT--EGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVK--GSDYNLKRNDLMKKEDVLNVKDLDCWEPS 708
            RRR+++ HGHC  DD +W PH+  +EP YT+ GPDW+SSLRYIPSP++E  KNE LWPEKSSH+RT+P     +   W+  +       GKKC F   + H +S+YSCD +   +  G KRK   ID RNGF+  + GD VYQA EY+PDF+K+G TVP V FG     K +TFIP+FP  K  G DY++K      +ED L V  L+ W P+
Sbjct:   38 RRRRFLHHGHCTVDDTAWKPHAMAVEPAYTDCGPDWKSSLRYIPSPRDERRKNEELWPEKSSHLRTFPGSNGCSAMEWS--YYTNGYPTGKKCFFGSEKQHKRSEYSCDHVDFDATMGCKRKESEIDRRNGFDSAAHGDNVYQAAEYAPDFFKHGSTVPAVNFGGNGSRKAETFIPLFPLEKPLGPDYSVKARVRRVREDELEVAALERWRPA 248
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. TrEMBL
Match: A0A267DN64 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009874g2 PE=4 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 1.431e-63
Identity = 104/213 (48.83%), Postives = 136/213 (63.85%), Query Frame = 1 
Query:   82 RRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDKGKKCIFT--EGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVK--GSDYNLKRNDLMKKEDVLNVKDLDCWEPS 708
            RRR+++ HGHC  DD +W PH+  +EP YT+ GPDW+SSLRYIPSP++E  KNE LWPEKSSH+RT+P     +   W+  +       GKKC F   + H +S+YSCD +   +  G KRK   ID RNGF+  + GD VYQA EY+PDF+K+G TVP V FG     K +TFIP+FP  K  G DY++K      +ED L V  L+ W P+
Sbjct:   40 RRRRFLHHGHCTVDDTAWKPHAMAVEPAYTDCGPDWKSSLRYIPSPRDERRKNEELWPEKSSHLRTFPGSNGCSAMEWS--YYTNGYPTGKKCFFGSEKQHKRSEYSCDHVDFDATMGCKRKESEIDRRNGFDSAAHGDNVYQAAEYAPDFFKHGSTVPAVNFGGNGTRKAETFIPLFPLEKPLGPDYSVKARVRRVREDELEVAALERWRPA 250
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. TrEMBL
Match: A0A267DPW3 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009874g1 PE=4 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 1.527e-63
Identity = 104/213 (48.83%), Postives = 136/213 (63.85%), Query Frame = 1 
Query:   82 RRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDKGKKCIFT--EGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVK--GSDYNLKRNDLMKKEDVLNVKDLDCWEPS 708
            RRR+++ HGHC  DD +W PH+  +EP YT+ GPDW+SSLRYIPSP++E  KNE LWPEKSSH+RT+P     +   W+  +       GKKC F   + H +S+YSCD +   +  G KRK   ID RNGF+  + GD VYQA EY+PDF+K+G TVP V FG     K +TFIP+FP  K  G DY++K      +ED L V  L+ W P+
Sbjct:   40 RRRRFLHHGHCTVDDTAWKPHAMAVEPAYTDCGPDWKSSLRYIPSPRDERRKNEELWPEKSSHLRTFPGSNGCSAMEWS--YYTNGYPTGKKCFFGSEKQHKRSEYSCDHVDFDATMGCKRKESEIDRRNGFDSAAHGDNVYQAAEYAPDFFKHGSTVPAVNFGGNGSRKAETFIPLFPLEKPLGPDYSVKARVRRVREDELEVAALERWRPA 250
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. TrEMBL
Match: A0A267HB84 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig013838g1 PE=4 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 2.356e-63
Identity = 104/213 (48.83%), Postives = 136/213 (63.85%), Query Frame = 1 
Query:   82 RRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDKGKKCIFT--EGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVK--GSDYNLKRNDLMKKEDVLNVKDLDCWEPS 708
            RRR+++ HGHC  DD +W PH+  +EP YT+ GPDW+SSLRYIPSP++E  KNE LWPEKSSH+RT+P     +   W+  +       GKKC F   + H +S+YSCD +   +  G KRK   ID RNGF+  + GD VYQA EY+PDF+K+G TVP V FG     K +TFIP+FP  K  G DY++K      +ED L V  L+ W P+
Sbjct:   40 RRRRFLHHGHCTVDDTAWKPHAMAVEPAYTDCGPDWKSSLRYIPSPRDERRKNEELWPEKSSHLRTFPGSNGCSAMEWS--YYTNGYPTGKKCFFGSEKQHKRSEYSCDHVDFDATMGCKRKESEIDRRNGFDSAAHGDNVYQAAEYAPDFFKHGSTVPAVNFGGNGSRKAETFIPLFPLEKPLGPDYSVKARVRRVREDELEVAALERWRPA 250
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. TrEMBL
Match: A0A1I8G4J9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 2.525e-63
Identity = 104/213 (48.83%), Postives = 136/213 (63.85%), Query Frame = 1 
Query:   82 RRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDKGKKCIFT--EGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVK--GSDYNLKRNDLMKKEDVLNVKDLDCWEPS 708
            RRR+++ HGHC  DD +W PH+  +EP YT+ GPDW+SSLRYIPSP++E  KNE LWPEKSSH+RT+P     +   W+  +       GKKC F   + H +S+YSCD +   +  G KRK   ID RNGF+  + GD VYQA EY+PDF+K+G TVP V FG     K +TFIP+FP  K  G DY++K      +ED L V  L+ W P+
Sbjct:   38 RRRRFLHHGHCTVDDTAWKPHAMAVEPAYTDCGPDWKSSLRYIPSPRDERRKNEELWPEKSSHLRTFPGSNGCSAMEWS--YYTNGYPTGKKCFFGSEKQHKRSEYSCDHVDFDATMGCKRKESEIDRRNGFDSAAHGDNVYQAAEYAPDFFKHGSTVPAVNFGGNGSRKAETFIPLFPLEKPLGPDYSVKARVRRVREDELEVAALERWRPA 248
[Close]
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Cavefish
Match: ENSAMXT00000043064.1 (spermatogenesis associated serine rich 1 [Source:NCBI gene;Acc:103031286])

HSP 1 Score: 90.1225 bits (222), Expect = 3.386e-21
Identity = 68/217 (31.34%), Postives = 101/217 (46.54%), Query Frame = 1 
Query:  124 DKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGK----NESLWPEKSSHIRTYPSLLYSNYFVWN--PKF-ENIRQDKGKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVK--GSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISVLYTNPFS 747
             + + PH   +EP  T    DW+S LR +P P+  +       E  +P +    R +P    ++   W   P F + I    GK+C F       K    E T +S  G KR + N D R+     + G   + + EYSPDF+K+G TVP   FG  +  K DTFIP+ P  +     +  K+  L ++ED+L VK+L  W P+   I  +   P S
Sbjct:   13 QREFRPHIRHVEPVLTECDLDWKSRLRRVPEPRYSDAPFPQIKEIKFPSEIRLTRPFPQANMASQSEWTFYPNFGQPITYHAGKRCGFYHA---VKRCISEQTLESTIGRKRHV-NQDCRS-----AAGSSTFLSPEYSPDFHKHGATVPKASFGFPTIFKADTFIPLQPLPEKICLPHTEKKKILDREEDILEVKNLSKWRPAPQLIESIIEEPGS 220
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Nematostella
Match: EDO32558 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUP7])

HSP 1 Score: 53.1434 bits (126), Expect = 2.831e-8
Identity = 35/87 (40.23%), Postives = 42/87 (48.28%), Query Frame = 1 
Query:  454 DVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPI--FPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPS 708
            D RNG    S GD  YQ  EYS  F+K G T  VV FG       DTF+P+   P      +  K     K  ++L V DLD W+PS
Sbjct:  149 DSRNGIPVASPGDNSYQVPEYSRSFHKFGSTRSVVSFGGRLEYVPDTFVPLQDLPLRPRMSFLEKEIRQRKVNEILEVIDLDGWKPS 235
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Planmine SMEST
Match: SMESG000043323.1 (SMESG000043323.1)

HSP 1 Score: 494.582 bits (1272), Expect = 1.740e-179
Identity = 239/240 (99.58%), Postives = 240/240 (100.00%), Query Frame = 1 
Query:   43 MSFSKENSNQNHERRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDKGKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISVLYTNPFSKIKAN 762
            MSFSKENSNQNHERRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDKGKKCIFTEGHLKSKYSCDEITD+SKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISVLYTNPFSKIKAN
Sbjct:    1 MSFSKENSNQNHERRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWESSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQDKGKKCIFTEGHLKSKYSCDEITDKSKFGSKRKLQNIDVRNGFNDLSLGDKVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVKGSDYNLKRNDLMKKEDVLNVKDLDCWEPSKNAISVLYTNPFSKIKAN 240
The following BLAST results are available for this feature:
BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SPATS13.777e-1134.35spermatogenesis associated serine rich 1 [Source:H... [more]
SPATS13.777e-1134.35spermatogenesis associated serine rich 1 [Source:H... [more]
SPATS13.777e-1134.35spermatogenesis associated serine rich 1 [Source:H... [more]
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
BX255920.18.380e-825.00spermatogenesis associated serine rich 1 [Source:N... [more]
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hmcn22.309e-1830.73hemicentin 2 [Source:Xenbase;Acc:XB-GENE-6034990][more]
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Spats15.246e-934.96spermatogenesis associated, serine-rich 1 [Source:... [more]
Spats18.725e-934.96spermatogenesis associated, serine-rich 1 [Source:... [more]
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q496A3|SPAS1_HUMAN1.814e-1034.35Spermatogenesis-associated serine-rich protein 1 O... [more]
sp|A2RRY8|SPAS1_MOUSE3.669e-834.96Spermatogenesis-associated serine-rich protein 1 O... [more]
sp|Q811V6|SPAS1_RAT8.448e-834.96Spermatogenesis-associated serine-rich protein 1 O... [more]
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8FV881.222e-6348.83Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DN641.431e-6348.83Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267DPW31.527e-6348.83Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267HB842.356e-6348.83Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8G4J92.525e-6348.83Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000043064.13.386e-2131.34spermatogenesis associated serine rich 1 [Source:N... [more]
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO325582.831e-840.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of spermatogenesis-associated serine-rich protein 1-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000043323.11.740e-17999.58SMESG000043323.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017224 ID=SMED30017224|Name=spermatogenesis-associated serine-rich protein 1-like|organism=Schmidtea mediterranea sexual|type=transcript|length=874bp
TGTTTATATCTATTACAATTTTGTTGTGTTGGAAATTAAATAATGAGTTT
TTCAAAAGAAAATTCCAACCAAAATCATGAACGTAGAAGGAAATATATAT
CACATGGACATTGTGTAGAGGATGACAAAAGCTGGATCCCACACAGCAAT
CCAATTGAACCGACTTATACAAATTCGGGTCCCGACTGGGAATCATCCTT
GCGATATATCCCATCTCCGAAAAACGAGGAGGGTAAAAATGAAAGTTTAT
GGCCAGAAAAATCCAGCCACATTCGAACTTATCCAAGTCTTCTCTACAGC
AATTATTTCGTTTGGAATCCGAAGTTTGAAAATATCCGACAAGATAAAGG
GAAAAAATGCATTTTCACAGAGGGCCATTTGAAAAGCAAATATAGTTGCG
ATGAAATCACGGACCAATCAAAATTTGGCTCAAAACGCAAATTGCAAAAT
ATTGATGTTCGTAACGGATTTAATGATCTTTCTTTGGGAGACAAAGTCTA
TCAGGCCGTTGAATACTCTCCAGACTTTTACAAGAACGGAGTGACCGTCC
CTGTGGTTCCATTTGGAAGAATATCGAGAGCTAAGCATGATACTTTCATT
CCGATTTTCCCATATGTTAAAGGGTCTGACTATAACCTCAAACGAAATGA
CTTGATGAAAAAAGAAGATGTACTAAATGTAAAAGATTTGGACTGTTGGG
AGCCAAGCAAAAATGCCATTAGTGTGTTGTACACGAATCCTTTTTCGAAA
ATCAAAGCCAATTAGTAGACTTTATCGTTACTGTTGATCCTTAACTTCAT
GAAAGTTGGGAATCGCGTTCCAATAAGATCTTTCTCATATAATAAATTGA
CAATTTTGTTGTCTTGAGCCAAAA
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protein sequence of SMED30017224-orf-1

>SMED30017224-orf-1 ID=SMED30017224-orf-1|Name=SMED30017224-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=241bp
MSFSKENSNQNHERRRKYISHGHCVEDDKSWIPHSNPIEPTYTNSGPDWE
SSLRYIPSPKNEEGKNESLWPEKSSHIRTYPSLLYSNYFVWNPKFENIRQ
DKGKKCIFTEGHLKSKYSCDEITDQSKFGSKRKLQNIDVRNGFNDLSLGD
KVYQAVEYSPDFYKNGVTVPVVPFGRISRAKHDTFIPIFPYVKGSDYNLK
RNDLMKKEDVLNVKDLDCWEPSKNAISVLYTNPFSKIKAN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR029165SASRP1
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029165Spermatogenesis-associated serine-rich protein 1PFAMPF15160SASRP1coord: 97..226
e-value: 8.1E-16
score: 58.4
IPR029165Spermatogenesis-associated serine-rich protein 1PANTHERPTHR35845FAMILY NOT NAMEDcoord: 6..231