SFI1

Overview
NameSFI1 (AFB74723.1)
Smed IDSMED30017201
Length (bp)3564
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SFI1 (SMED30017201) t-SNE clustered cells

Violin plots show distribution of expression levels for SFI1 (SMED30017201) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SFI1 (SMED30017201) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SFI1 (SMED30017201) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30017201

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30017201h1SMcG0010524 SmedASXL_016974SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30017201h1SMcG0010524 dd_Smed_v4_12484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30017201h1SMcG0010524 dd_Smed_v4_12484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30017201h1SMcG0010524 dd_Smed_v4_12484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
non-ciliated neuronSMED30017201h1SMcG0010524 dd_Smed_v4_12484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30017201h1SMcG0010524 dd_Smed_v6_12484_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SFI1 vs. Ensembl Human
Match: SFI1 (SFI1 centrin binding protein [Source:HGNC Symbol;Acc:HGNC:29064])

HSP 1 Score: 100.523 bits (249), Expect = 1.233e-20
Identity = 162/722 (22.44%), Postives = 288/722 (39.89%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFH-RDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDS----HYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQ----EMRRRFQ----LRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSF--ANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMIC--LRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQ 2483
            Y   +   R GRL+ELRIRC ARKF Y+W   + G+V    A  +Y +RL R  ++ W +++W  + E +L  RA   ++     L+F TW  + R + E  RN  +  + H      R A  +W+ ++ V++ K +++  A +   +I L+  +  W                                    R  + Q +++R    +      L L  Q W ++  +    QKEK++  S    H +W    R+L  W        +EY    R +    EM  RF     L+ YF  WQ+ +   + L       +++   +  RR + H+K ++L   E+ +  E A+     ++L+L R+ + W S++E+K+       + AA  HYR         +  K    W Q+T     +    + A   F+ R L  A  +W+        ++ L      F R+ +  + ++ WRQ                             K F   + +L E + +     HA R+LL + +    Q     HQ +    +A  H +     K F +WR     LR +   ++    FH  +  + R  ++ W E        R+++       ++ ++ +   AW TY+   +   R+ + R  +  R    +L R    +WK  T  R+   +K+  A   YR     K L  W++  S+Q
Sbjct:   71 YRGTHTCTRQGRLRELRIRCVARKFLYLWIRMTFGRVFPSKARFYYEQRLLRKVFEEWKEEWWVFQHEWKLCVRADCHYRYYLYNLMFQTWKTYVRQQQE-MRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRIILRVWWSTW------------------------------------RQRLGQVRVSRALHASALKHRALSLQVQAWSQWREQLLYVQKEKQKVVSAVKHHQHWQ-KRRFLKAW--------LEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKMALRRAFTHWKHYMLLCAEEAAQFEMAEEHHRHSQLLLHRFWNLWRSQIEQKKERELLPLLHAAWDHYRI-------ALLCKCIELWLQYTQKRRYKQLLQARADGHFQQRALPAAFHTWNRLWRWRHQENVLSARATRFHRETLEKQVFSLWRQ-----------------------------KMFQHRENRLAERMAI----LHAERQLLYRSWFMWHQQAAARHQEQEWQTVACAHHRHGRLKKAFCLWRESAQGLRTERTGRVRAAEFHMAQ--LLRWAWSQWRECLALRGAERQKLMRADLHHQHSVLHRALQAWVTYQGRVRSILREVAARESQHNR----QLLRGALRRWKENTMARVDEAKKTFQASTHYRRTICSKVLVQWREAVSVQ 700          
BLAST of SFI1 vs. Ensembl Human
Match: SFI1 (SFI1 centrin binding protein [Source:HGNC Symbol;Acc:HGNC:29064])

HSP 1 Score: 92.8189 bits (229), Expect = 2.675e-18
Identity = 154/717 (21.48%), Postives = 286/717 (39.89%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFH-RDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKEN---------AQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMIC--LRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQ 2483
            Y   +   R GRL+ELRIRC ARKF Y+W   + G+V    A  +Y +RL R  ++ W +++W  + E +L  RA   ++     L+F TW  + R + E  RN  +  + H      R A  +W+ ++ V++ K +++  A +   +I L+  +  W+          A  A ++ H    L  +   QW      V++ K   + +  H      +   + W EY    ++K+++ E  +  ++  +   Y   W+Q        YA   + +++ R+  LR+ F  W+ Y         Q ++A++ +          H + +   R  K+++            A       +L R+ + W S++E+K+       + AA  HYR         +  K    W Q+T     +    + A   F+ R L  A  +W+        ++ L      F R+ +  + ++ WRQ                             K F   + +L E + +     HA R+LL + +    Q     HQ +    +A  H +     K F +WR     LR +   ++    FH  +  + R  ++ W E        R+++       ++ ++ +   AW TY+   +   R+ + R  +  R    +L R    +WK  T  R+   +K+  A   YR     K L  W++  S+Q
Sbjct:   71 YRGTHTCTRQGRLRELRIRCVARKFLYLWIRMTFGRVFPSKARFYYEQRLLRKVFEEWKEEWWVFQHEWKLCVRADCHYRYYLYNLMFQTWKTYVRQQQE-MRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRIILRVWWSTWRQRLGQVRVSRALHASALKHRALSLQVQAWSQWREQLLYVQKEKQKVVSAVKHHQHWQKRRFLKAWLEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKMALRRAFTHWKHYMLLCAEEAAQFEMAEEHHR---------HSQLYFCFRALKDNVTHAHLQQIRRNLAHQQHGVTLLHRFWNLWRSQIEQKKERELLPLLHAAWDHYRI-------ALLCKCIELWLQYTQKRRYKQLLQARADGHFQQRALPAAFHTWNRLWRWRHQENVLSARATRFHRETLEKQVFSLWRQ-----------------------------KMFQHRENRLAERMAI----LHAERQLLYRSWFMWHQQAAARHQEQEWQTVACAHHRHGRLKKAFCLWRESAQGLRTERTGRVRAAEFHMAQ--LLRWAWSQWRECLALRGAERQKLMRADLHHQHSVLHRALQAWVTYQGRVRSILREVAARESQHNR----QLLRGALRRWKENTMARVDEAKKTFQASTHYRRTICSKVLVQWREAVSVQ 731          
BLAST of SFI1 vs. Ensembl Human
Match: SFI1 (SFI1 centrin binding protein [Source:HGNC Symbol;Acc:HGNC:29064])

HSP 1 Score: 70.8626 bits (172), Expect = 1.397e-11
Identity = 154/722 (21.33%), Postives = 276/722 (38.23%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFH-RDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDS----HYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQ----EMRRRFQ----LRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSF--ANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMIC--LRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQ 2483
            Y   +   R GRL+ELRIR      FY                  Y +RL R  ++ W +++W  + E +L  RA   ++     L+F TW  + R + E  RN  +  + H      R A  +W+ ++ V++ K +++  A +   +I L+  +  W                                    R  + Q +++R    +      L L  Q W ++  +    QKEK++  S    H +W    R+L  W        +EY    R +    EM  RF     L+ YF  WQ+ +   + L       +++   +  RR + H+K ++L   E+ +  E A+     ++L+L R+ + W S++E+K+       + AA  HYR         +  K    W Q+T     +    + A   F+ R L  A  +W+        ++ L      F R+ +  + ++ WRQ                             K F   + +L E + +     HA R+LL + +    Q     HQ +    +A  H +     K F +WR     LR +   ++    FH  +  + R  ++ W E        R+++       ++ ++ +   AW TY+   +   R+ + R  +  R    +L R    +WK  T  R+   +K+  A   YR     K L  W++  S+Q
Sbjct:   71 YRGTHTCTRQGRLRELRIR------FY------------------YEQRLLRKVFEEWKEEWWVFQHEWKLCVRADCHYRYYLYNLMFQTWKTYVRQQQE-MRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRIILRVWWSTW------------------------------------RQRLGQVRVSRALHASALKHRALSLQVQAWSQWREQLLYVQKEKQKVVSAVKHHQHWQ-KRRFLKAW--------LEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKMALRRAFTHWKHYMLLCAEEAAQFEMAEEHHRHSQLLLHRFWNLWRSQIEQKKERELLPLLHAAWDHYRI-------ALLCKCIELWLQYTQKRRYKQLLQARADGHFQQRALPAAFHTWNRLWRWRHQENVLSARATRFHRETLEKQVFSLWRQ-----------------------------KMFQHRENRLAERMAI----LHAERQLLYRSWFMWHQQAAARHQEQEWQTVACAHHRHGRLKKAFCLWRESAQGLRTERTGRVRAAEFHMAQ--LLRWAWSQWRECLALRGAERQKLMRADLHHQHSVLHRALQAWVTYQGRVRSILREVAARESQHNR----QLLRGALRRWKENTMARVDEAKKTFQASTHYRRTICSKVLVQWREAVSVQ 676          
BLAST of SFI1 vs. Ensembl Human
Match: SFI1 (SFI1 centrin binding protein [Source:HGNC Symbol;Acc:HGNC:29064])

HSP 1 Score: 66.6254 bits (161), Expect = 1.255e-10
Identity = 48/159 (30.19%), Postives = 83/159 (52.20%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIG-RLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWK 842
            Y   +   R GRL+ELRIRC ARKF Y+W   + G+V    A  +Y +RL R  ++ W +++W  + E +L  RA   ++     L+F TW  + R + E  RN  +  + H      R A  +W+ ++ V++ K +++  A +   +I L+  +  W+
Sbjct:   71 YRGTHTCTRQGRLRELRIRCVARKFLYLWIRMTFGRVFPSKARFYYEQRLLRKVFEEWKEEWWVFQHEWKLCVRADCHYRYYLYNLMFQTWKTYVRQQQE-MRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRIILRVWWSTWR 228          
BLAST of SFI1 vs. Ensembl Human
Match: SFI1 (SFI1 centrin binding protein [Source:HGNC Symbol;Acc:HGNC:29064])

HSP 1 Score: 58.151 bits (139), Expect = 1.140e-7
Identity = 136/676 (20.12%), Postives = 263/676 (38.91%), Query Frame = 3
Query:  501 HYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFH-RDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYY---GEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKEN---------AQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMIC--LRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQ 2483
            +Y +RL R  ++ W +++W  + E +L  RA   ++     L+F TW  + R + E  RN  +  + H      R A  +W+ ++ V++ K +++  A +   +I L+  +  W+          A  A ++ H    L  +   QW      V++ K   + +  H      +   + W EY    ++K+++ E  +  ++  +   Y   W+Q        YA   + +++ R+  LR+ F  W+ Y     EE     Q ++A++ +          H + +   R  K+++            A       +L R+ + W S++E+K+       + AA  HYR         +  K    W Q+T     +    + A   F+ R L  A  +W+        ++ L      F R+ +  + ++ WRQ                             K F   + +L E + +     HA R+LL + +    Q     HQ +    +A  H +     K F +WR     LR +   ++    FH  +  + R  ++ W E        R+++       ++ ++ +   AW TY+   +   R+ + R  +  R    +L R    +WK  T  R+   +K+  A   YR     K L  W++  S+Q
Sbjct:   33 YYEQRLLRKVFEEWKEEWWVFQHEWKLCVRADCHYRYYLYNLMFQTWKTYVRQQQE-MRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRIILRVWWSTWRQRLGQVRVSRALHASALKHRALSLQVQAWSQWREQLLYVQKEKQKVVSAVKHHQHWQKRRFLKAWLEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKMALRRAFTHWKHYMLLCAEEA---AQFEMAEEHHR---------HSQLYFCFRALKDNVTHAHLQQIRRNLAHQQHGVTLLHRFWNLWRSQIEQKKERELLPLLHAAWDHYRI-------ALLCKCIELWLQYTQKRRYKQLLQARADGHFQQRALPAAFHTWNRLWRWRHQENVLSARATRFHRETLEKQVFSLWRQ-----------------------------KMFQHRENRLAERMAI----LHAERQLLYRSWFMWHQQAAARHQEQEWQTVACAHHRHGRLKKAFCLWRESAQGLRTERTGRVRAAEFHMAQ--LLRWAWSQWRECLALRGAERQKLMRADLHHQHSVLHRALQAWVTYQGRVRSILREVAARESQHNR----QLLRGALRRWKENTMARVDEAKKTFQASTHYRRTICSKVLVQWREAVSVQ 649          

HSP 2 Score: 53.5286 bits (127), Expect = 2.750e-6
Identity = 82/378 (21.69%), Postives = 157/378 (41.53%), Query Frame = 3
Query: 1050 HKTKIKQKEKERWDS--HYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNC---WIEYTN 2168
            H+  IKQ+ +++ DS  +Y   L  +   +WK+       E+ +  R     R +     F  W+ Y  +++ +R +   A+  +     R+ W  +  +++ RR K  ++  A  F  ++ILR +   W  RL +   V     + A+   +R         + ++ +S+W +         Q + +A+K  +       + +W  ++ + + K +   + E F    VL  Y+  W+Q  + RE+L     Q  ++ +     + F  W     +   E  Q    + H     L  CF AL  NV   H ++I  +LA       L ++ + +WR    +KK +  L L       YRI  ++  C   W++YT 
Sbjct:   16 HQKVIKQRMEKKVDSRFYYEQRLLRKVFEEWKEEWWVFQHEWKLCVRADCHYRYYLYNLMFQTWKTYVRQQQEMRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRIILRVWWSTWRQRLGQ---VRVSRALHASALKHRALS------LQVQAWSQWREQLLYVQKEKQKVVSAVKHHQHWQKRRFLKAWLEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKMALRRAFTHWKHYMLLCAEEAAQFEMAEEHHRHSQLYFCFRALKDNVTHAHLQQIRRNLAHQQHGVTLLHRFWNLWRSQIEQKKERELLPLLHAAWDHYRIA-LLCKCIELWLQYTQ 383          
BLAST of SFI1 vs. Ensembl Zebrafish
Match: sfi1 (SFI1 centrin binding protein [Source:ZFIN;Acc:ZDB-GENE-080206-4])

HSP 1 Score: 91.6633 bits (226), Expect = 4.381e-18
Identity = 178/859 (20.72%), Postives = 346/859 (40.28%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRI---LAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFIL-----QRREKNSIKE--------NAQSFA-----------NKLILRRYTDK---------WISRLEEKENVNTKTEMGAARSHYRFP----------KRDSER------------VMHMKYFSK----WSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLC--KYWTKW------RQEKKARE--------------NLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFI-KWKAQTHVRLLAIQKSKIAD---DLYRANCLR-KYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRI------WLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHW 2660
            Y   Y WNRGGRLKELRIR  ARKF ++W  ++ G+ T   A  HY + + +  ++ W  ++W  R E  L  RA   +        F    ++  ++ E  + +++A  F  ++  R    +W   +++  C+ K R+     + +++ +  K+ + +W +A Q +     +  + + H+   +  +    WL  R        +RMK                     H   +K+KE +    HY   L  + L  W +H  +   +       Q++RR   L +++ QW +     +V + + + AD++ +   +R V+ H++ ++       +REK+++            + FA           NK I  ++  +         W  RL+ +EN   + +M  A++H++F           KR +E                 + F +    W +FT+    + +    A + +  +       +W  +  +   + R +  +   +    +C  + W+KW      R+E++ ++              NL    N+++ IQ  QKR K                    + + H  ++ L +  T   Q V     +K  I     H+  KL       W+   L+ +  ++L+ + + + E  + R +F  W     Q+ + RE+        +  +V Q F AW+         + + +  L + Q +L+ KL+  + + KWK     R + ++  ++A    D + +  LR K L  W  +  I   + +Q  K   F    EL ++      ++ W+  L  R+RE   T  AL HW+  L+AK+F  W
Sbjct:   31 YRVGYTWNRGGRLKELRIRHLARKFLFLWIRRTFGRKTTSQARSHYCKVILKKVFRSWKDEWWHSRKEWTLNIRADCHYAYTVYSKTFQALQEYVAVQREEKKKLQLAITFETEHKLRCVWNSWE--LYLDMCRMKRRMQEAAVQHKNLTVQ-KWVWTVWNLALQHRKLEYHQQDLGLQHWAHSVQSRA---WLHWR--------DRMK---------------------HACALKEKESKA-HHHYCLQLLKQTLHGWIRHAENRQAKNKKIAVAQDVRRLSVLERFWWQWFEVLQSRRVEKDRGQRADKLAQHGSQRLVFSHWRHYVSICSQKAKREKSAMHHRQRYLLHLGLKGFALNVTQSKTYRLNKNISVQHRHQNLLAMCWNVWQLRLDREENRRLQPQMSVAQNHHKFSVLRFNLHHWKKRLAEHKKCQELELRADACFAQRIFPQCVNTWMEFTAQRTEKREQKERAEQQYRQQTFSWVFYTW--WRNLEARRDRRLAERTAVLHAEHVCYVRVWSKWYSGAVQRREERIKQAVADSLYKHTLQQKNLNHWKNRFINIQTSQKRFK--------------------QAEGHHEQRCLKRAMTDWHQYVDHRRLKKEKIAEMEKHYHNKLLKHALDAWKSYHLQTQAISQLVEYSYQEHEQHLVRRMFCLWRINVLQLVEEREKENRATCLSQKHLVSQVFLAWQQRTVSAHLHRHKKNEALKQAQTQLE-KLRIQVALRKWKE----RSIEVKDERLASERADRHYSRTLREKTLSNWTSF--IHHHKIYQVMKKRSF----ELHKLRTCQHFFICWKTQLQSRRREAEQTETALWHWSLNLQAKVFCMW 820          
BLAST of SFI1 vs. Ensembl Zebrafish
Match: sfi1 (SFI1 centrin binding protein [Source:ZFIN;Acc:ZDB-GENE-080206-4])

HSP 1 Score: 90.8929 bits (224), Expect = 6.636e-18
Identity = 178/859 (20.72%), Postives = 346/859 (40.28%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRI---LAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFIL-----QRREKNSIKE--------NAQSFA-----------NKLILRRYTDK---------WISRLEEKENVNTKTEMGAARSHYRFP----------KRDSER------------VMHMKYFSK----WSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLC--KYWTKW------RQEKKARE--------------NLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFI-KWKAQTHVRLLAIQKSKIAD---DLYRANCLR-KYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRI------WLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHW 2660
            Y   Y WNRGGRLKELRIR  ARKF ++W  ++ G+ T   A  HY + + +  ++ W  ++W  R E  L  RA   +        F    ++  ++ E  + +++A  F  ++  R    +W   +++  C+ K R+     + +++ +  K+ + +W +A Q +     +  + + H+   +  +    WL  R        +RMK                     H   +K+KE +    HY   L  + L  W +H  +   +       Q++RR   L +++ QW +     +V + + + AD++ +   +R V+ H++ ++       +REK+++            + FA           NK I  ++  +         W  RL+ +EN   + +M  A++H++F           KR +E                 + F +    W +FT+    + +    A + +  +       +W  +  +   + R +  +   +    +C  + W+KW      R+E++ ++              NL    N+++ IQ  QKR K                    + + H  ++ L +  T   Q V     +K  I     H+  KL       W+   L+ +  ++L+ + + + E  + R +F  W     Q+ + RE+        +  +V Q F AW+         + + +  L + Q +L+ KL+  + + KWK     R + ++  ++A    D + +  LR K L  W  +  I   + +Q  K   F    EL ++      ++ W+  L  R+RE   T  AL HW+  L+AK+F  W
Sbjct:   31 YRVGYTWNRGGRLKELRIRHLARKFLFLWIRRTFGRKTTSQARSHYCKVILKKVFRSWKDEWWHSRKEWTLNIRADCHYAYTVYSKTFQALQEYVAVQREEKKKLQLAITFETEHKLRCVWNSWE--LYLDMCRMKRRMQEAAVQHKNLTVQ-KWVWTVWNLALQHRKLEYHQQDLGLQHWAHSVQSRA---WLHWR--------DRMK---------------------HACALKEKESKA-HHHYCLQLLKQTLHGWIRHAENRQAKNKKIAVAQDVRRLSVLERFWWQWFEVLQSRRVEKDRGQRADKLAQHGSQRLVFSHWRHYVSICSQKAKREKSAMHHRQRYLLHLGLKGFALNVTQSKTYRLNKNISVQHRHQNLLAMCWNVWQLRLDREENRRLQPQMSVAQNHHKFSVLRFNLHHWKKRLAEHKKCQELELRADACFAQRIFPQCVNTWMEFTAQRTEKREQKERAEQQYRQQTFSWVFYTW--WRNLEARRDRRLAERTAVLHAEHVCYVRVWSKWYSGAVQRREERIKQAVADSLYKHTLQQKNLNHWKNRFINIQTSQKRFK--------------------QAEGHHEQRCLKRAMTDWHQYVDHRRLKKEKIAEMEKHYHNKLLKHALDAWKSYHLQTQAISQLVEYSYQEHEQHLVRRMFCLWRINVLQLVEEREKENRATCLSQKHLVSQVFLAWQQRTVSAHLHRHKKNEALKQAQTQLE-KLRIQVALRKWKE----RSIEVKDERLASERADRHYSRTLREKTLSNWTSF--IHHHKIYQVMKKRSF----ELHKLRTCQHFFICWKTQLQSRRREAEQTETALWHWSLNLQAKVFCMW 820          
BLAST of SFI1 vs. Ensembl Zebrafish
Match: sfi1 (SFI1 centrin binding protein [Source:ZFIN;Acc:ZDB-GENE-080206-4])

HSP 1 Score: 87.8113 bits (216), Expect = 6.669e-17
Identity = 168/807 (20.82%), Postives = 329/807 (40.77%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRI---LAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFIL-----QRREKNSIKE--------NAQSFA-----------NKLILRRYTDK---------WISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLC-KYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVW--RMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCL-RKYLDAW---KQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHW 2660
            Y   Y WNRGGRLKELRIR  ARKF ++W  ++ G+ T   A  HY + + +  ++ W  ++W  R E  L  RA   +        F    ++  ++ E  + +++A  F  ++  R    +W   +++  C+ K R+     + +++ +  K+ + +W +A Q +     +  + + H+   +  +    WL  R        +RMK                     H   +K+KE +    HY   L  + L  W +H  +   +       Q++RR   L +++ QW +     +V + + + AD++ +   +R V+ H++ ++       +REK+++            + FA           NK I  ++  +         W  RL+ +EN   + +M  A++H++F       V+       W +  +      +    A   F  R+    +++W  F      K       E   R+      ++T WR       NLE + +     +++ +R  + H           E++   RV         SK ++  +Q      + +I   +A   ++  LQ K    W  R I ++   Q +      H  +  +KR + + W +Y +  +  +E++  M +   N ++     AW++Y  + Q   +   Y   E ++     L R +F  W+    + +L + + +  ++  RA CL +K+L +    K+ + + +LR  Q                ++ W+  L  R+RE   T  AL HW+  L+AK+F  W
Sbjct:   31 YRVGYTWNRGGRLKELRIRHLARKFLFLWIRRTFGRKTTSQARSHYCKVILKKVFRSWKDEWWHSRKEWTLNIRADCHYAYTVYSKTFQALQEYVAVQREEKKKLQLAITFETEHKLRCVWNSWE--LYLDMCRMKRRMQEAAVQHKNLTVQ-KWVWTVWNLALQHRKLEYHQQDLGLQHWAHSVQSRA---WLHWR--------DRMK---------------------HACALKEKESKA-HHHYCLQLLKQTLHGWIRHAENRQAKNKKIAVAQDVRRLSVLERFWWQWFEVLQSRRVEKDRGQRADKLAQHGSQRLVFSHWRHYVSICSQKAKREKSAMHHRQRYLLHLGLKGFALNVTQSKTYRLNKNISVQHRHQNLLAMCWNVWQLRLDREENRRLQPQMSVAQNHHKFS------VLRFN-LHHWKKRLAEHKKCQELELRADACFAQRIFPQCVNTWMEFTAQRTEKREQKERAEQQYRQQTFSWVFYTWWR-------NLEARRD-----RRLAERTAVLHA----------EHVCYVRV--------WSKWYSGAVQR----REERIKQAVADSLYKHTLQQKNLNHWKNRFINIQTS-QKRFKQAEGHHEQRCLKRAMTD-WHQYVDHRRLKKEKIAEMEKHYHNKLLKHALDAWKSYHLQTQAISQLVEYSYQEHEQ----HLVRRMFCLWR----INVLQLVEEREKEN--RATCLSQKHLVSQVMKKRSFELHKLRTCQ--------------HFFICWKTQLQSRRREAEQTETALWHWSLNLQAKVFCMW 734          
BLAST of SFI1 vs. Ensembl Xenopus
Match: SFI1 (SFI1 centrin binding protein [Source:NCBI gene;Acc:100380177])

HSP 1 Score: 86.6557 bits (213), Expect = 8.028e-18
Identity = 63/231 (27.27%), Postives = 119/231 (51.52%), Query Frame = 3
Query:  360 LKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQW--LCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREY 1046
            L PY   Y WNRGGRLKELRIR  ARKF Y+W  K+ G+V    A  HY RRL    +  W +++W L +E +L  RA   ++ V  ++ F  W K+  L+  +   ++ A    + ++   A+ +W  ++ +++ K ++ ++A +   +  L+ ++ +W +  + +        ++++H+   L  +   QW  + V  I  + K+  +++  H     L+ A + W+ Y
Sbjct:   39 LIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKV--LQAVKHNRKCKLQAALKAWQLY 267          
BLAST of SFI1 vs. Ensembl Xenopus
Match: SFI1 (SFI1 centrin binding protein [Source:NCBI gene;Acc:100380177])

HSP 1 Score: 83.5741 bits (205), Expect = 3.079e-17
Identity = 60/200 (30.00%), Postives = 99/200 (49.50%), Query Frame = 3
Query:  360 LKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQW---LCVR 950
            L PY   Y WNRGGRLKELRIR  ARKF Y+W  K+ G+V    A  HY RRL    +  W +++W L +E +L  RA   ++ V  ++ F  W K+  L+  +   ++ A    + ++   A+ +W  ++ +++ K ++ ++A  Q            W+  +  +     K+  +V H  +  LQ  +  W   LC R
Sbjct:   31 LIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIAWLQ------------WREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYR 218          
BLAST of SFI1 vs. Ensembl Mouse
Match: Sfi1 (Sfi1 homolog, spindle assembly associated (yeast) [Source:MGI Symbol;Acc:MGI:1926137])

HSP 1 Score: 83.9593 bits (206), Expect = 1.130e-15
Identity = 177/811 (21.82%), Postives = 329/811 (40.57%), Query Frame = 3
Query:  402 RLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRL--AITNWVAWIFVKKCKFKLRILA---EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWK---------QHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQE-KKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWR-MICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVI-QTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRG-------------------------WQA------QKSSLFWQKSELI------RIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYT 2672
            RL+ELRIRC ARKF Y+W   + G+VT   A   + +++ +  +  W +++W  + E +L  RA   ++     L+F  W  +   + E  +   +A   H D   ++  A  +W+ ++  ++ K  ++  A    +QSV   L F +  W+         +A  A++V H    L  +   +W     I ++ +     +  H+     + + + W +Y    ++K+ + E     +   +   +   W+           H  L+V+ A          R  LR+ F  W+ Y   +     Q++ A +  +  +    +  FK  + Q R + + K+ AQ   +  +L R+ + W SR+E++E       + AA SHYR        V+H K    W ++      +    + A   F+ R L  A  +W+   +  + +  L      F R  +  + +  WRQ+  + REN   +    ++ ++ Q   + +  W     V   E  Q      H +  LL + F    ++ Q     ++    A+     +L ++ + +WR  + LR + Q KL     H     ++R +   W+ Y N+++ +  +V    ERQ N  ++    H WR     R    ++TS       R L  K    + ++W+  T V++   QK   A    R    R  L  W Q++     R                          W+A      +K  L  Q ++L+        +  WR+ LA R++E + T  AL  WA  L+AK++T W  + 
Sbjct:   58 RLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAE--HHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSV---LCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAG---------RMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVT------VLH-KCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQ-QLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQK-WLVYQNRVRSVLREVAAR-ERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSK----VLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFV 840          

HSP 2 Score: 65.4698 bits (158), Expect = 4.922e-10
Identity = 74/336 (22.02%), Postives = 153/336 (45.54%), Query Frame = 3
Query:  435 RKFFYIWRNKS----IGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILA--EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPI---VRQSKLNRMK-SNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENA-QSFANKLI 1409
            R+F+++W  ++    + +  + +A  H+   L R  + IW +     RIE     +A  FH +  +   ++ W +   LRLE  + ++ A    +  + R A+  W+  ++  + +  LR +A  E+Q  +  L +A  +W+     + +   K + +  HY++ L  KV+ QW  V  +    RQ +   ++ +    D   L+  FQ WR    +   ++ +  +   H++W L    + +WK HH   + +  +  +  ++  +   R  F QW+K     K  +     A  +    ++ +VW  +  F+L+RR K +  E A Q++  +L+
Sbjct:  529 RRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWL--VYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLL 862          
BLAST of SFI1 vs. Ensembl Mouse
Match: Sfi1 (Sfi1 homolog, spindle assembly associated (yeast) [Source:MGI Symbol;Acc:MGI:1926137])

HSP 1 Score: 83.5741 bits (205), Expect = 1.246e-15
Identity = 177/811 (21.82%), Postives = 329/811 (40.57%), Query Frame = 3
Query:  402 RLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRL--AITNWVAWIFVKKCKFKLRILA---EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWK---------QHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQE-KKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWR-MICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVI-QTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRG-------------------------WQA------QKSSLFWQKSELI------RIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYT 2672
            RL+ELRIRC ARKF Y+W   + G+VT   A   + +++ +  +  W +++W  + E +L  RA   ++     L+F  W  +   + E  +   +A   H D   ++  A  +W+ ++  ++ K  ++  A    +QSV   L F +  W+         +A  A++V H    L  +   +W     I ++ +     +  H+     + + + W +Y    ++K+ + E     +   +   +   W+           H  L+V+ A          R  LR+ F  W+ Y   +     Q++ A +  +  +    +  FK  + Q R + + K+ AQ   +  +L R+ + W SR+E++E       + AA SHYR        V+H K    W ++      +    + A   F+ R L  A  +W+   +  + +  L      F R  +  + +  WRQ+  + REN   +    ++ ++ Q   + +  W     V   E  Q      H +  LL + F    ++ Q     ++    A+     +L ++ + +WR  + LR + Q KL     H     ++R +   W+ Y N+++ +  +V    ERQ N  ++    H WR     R    ++TS       R L  K    + ++W+  T V++   QK   A    R    R  L  W Q++     R                          W+A      +K  L  Q ++L+        +  WR+ LA R++E + T  AL  WA  L+AK++T W  + 
Sbjct:   58 RLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAE--HHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSV---LCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAG---------RMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVT------VLH-KCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQ-QLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQK-WLVYQNRVRSVLREVAAR-ERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSK----VLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFV 840          

HSP 2 Score: 65.4698 bits (158), Expect = 4.909e-10
Identity = 74/336 (22.02%), Postives = 153/336 (45.54%), Query Frame = 3
Query:  435 RKFFYIWRNKS----IGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILA--EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPI---VRQSKLNRMK-SNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENA-QSFANKLI 1409
            R+F+++W  ++    + +  + +A  H+   L R  + IW +     RIE     +A  FH +  +   ++ W +   LRLE  + ++ A    +  + R A+  W+  ++  + +  LR +A  E+Q  +  L +A  +W+     + +   K + +  HY++ L  KV+ QW  V  +    RQ +   ++ +    D   L+  FQ WR    +   ++ +  +   H++W L    + +WK HH   + +  +  +  ++  +   R  F QW+K     K  +     A  +    ++ +VW  +  F+L+RR K +  E A Q++  +L+
Sbjct:  529 RRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWL--VYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLL 862          
BLAST of SFI1 vs. Ensembl Mouse
Match: Sfi1 (Sfi1 homolog, spindle assembly associated (yeast) [Source:MGI Symbol;Acc:MGI:1926137])

HSP 1 Score: 81.6481 bits (200), Expect = 5.396e-15
Identity = 178/807 (22.06%), Postives = 323/807 (40.02%), Query Frame = 3
Query:  402 RLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRL--AITNWVAWIFVKKCKFKLRILA---EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDS---HYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANK--LILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQE-KKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWR-MICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVI-QTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRG-------------------------WQA------QKSSLFWQKSELI------RIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYT 2672
            RL+ELRIRC ARKF Y+W   + G+VT   A   + +++ +  +  W +++W  + E +L  RA   ++     L+F  W  +   + E  +   +A   H D   ++  A  +W+ ++  ++ K  ++  A    +QSV   L F +  W+         +A  A++V H                                      L L  Q W  +  +  I Q+++ +  +   HY      R L  W ++     V+    +   +  R   L+ +F  WQ  +   + L   Q L  ++   ++ RR + H+K ++L + E+ + +E A        L+L R+ + W SR+E++E       + AA SHYR        V+H K    W ++      +    + A   F+ R L  A  +W+   +  + +  L      F R  +  + +  WRQ+  + REN   +    ++ ++ Q   + +  W     V   E  Q      H +  LL + F    ++ Q     ++    A+     +L ++ + +WR  + LR + Q KL     H     ++R +   W+ Y N+++ +  +V    ERQ N  ++    H WR     R    ++TS       R L  K    + ++W+  T V++   QK   A    R    R  L  W Q++     R                          W+A      +K  L  Q ++L+        +  WR+ LA R++E + T  AL  WA  L+AK++T W  + 
Sbjct:   58 RLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAE--HHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSV---LCFWWSKWRWRLGQAHAEHALHAVAVKHR------------------------------------ALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVT------VLH-KCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQ-QLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQK-WLVYQNRVRSVLREVAAR-ERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSK----VLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFV 809          
BLAST of SFI1 vs. Ensembl Mouse
Match: Sfi1 (Sfi1 homolog, spindle assembly associated (yeast) [Source:MGI Symbol;Acc:MGI:1926137])

HSP 1 Score: 75.0998 bits (183), Expect = 5.232e-13
Identity = 172/793 (21.69%), Postives = 326/793 (41.11%), Query Frame = 3
Query:  402 RLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRL--AITNWVAWIFVKKCKFKLRILA---EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWK---------QHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQE-KKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQ-LEHQRKINIDLASIH-----------WQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQI-----REQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKI--ADDLYRANCLRKYLDAWKQYY-SIQQLRGWQAQKSSLFWQKSELIRIWLI-WRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYT 2672
            RL+ELRIRC ARKF Y+W   + G+VT   A   + +++ +  +  W +++W  + E +L  RA   ++     L+F  W  +   + E  +   +A   H D   ++  A  +W+ ++  ++ K  ++  A    +QSV   L F +  W+         +A  A++V H    L  +   +W     I ++ +     +  H+     + + + W +Y    ++K+ + E     +   +   +   W+           H  L+V+ A          R  LR+ F  W+ Y   +     Q++ A +  +  +    +  FK  + Q R + + K+ AQ   +  +L R+ + W SR+E++E       + AA SHYR        V+H K    W ++      +    + A   F+ R L  A  +W+   +  + +  L      F R  +  + +  WRQ+  + REN   +    ++ ++ Q   + +  W     V   E  Q      H +  LL + F    ++ Q    +R      A  H           W++  QN+V  V R +  R++   + LLW              + W E  N M ++       Q ++ + R     V+     WR     + Y +++ +  L E ++ LD    ++ F  W+  +  +  A Q+ ++  A   +    L + +  WK ++    + +  Q Q + L  Q+  L R     WR+ LA R++E + T  AL  WA  L+AK++T W  + 
Sbjct:   58 RLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAE--HHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSV---LCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAG---------RMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVT------VLH-KCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQ-QLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLW-----------WALHLWRE--NTMARLDGAKKTSQARVHYSRTLCSKVLVQ---WREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCS--QRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQR--LSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFV 808          
BLAST of SFI1 vs. Ensembl Mouse
Match: Sfi1 (Sfi1 homolog, spindle assembly associated (yeast) [Source:MGI Symbol;Acc:MGI:1926137])

HSP 1 Score: 65.0846 bits (157), Expect = 5.697e-10
Identity = 74/336 (22.02%), Postives = 153/336 (45.54%), Query Frame = 3
Query:  435 RKFFYIWRNKS----IGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILA--EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPI---VRQSKLNRMK-SNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENA-QSFANKLI 1409
            R+F+++W  ++    + +  + +A  H+   L R  + IW +     RIE     +A  FH +  +   ++ W +   LRLE  + ++ A    +  + R A+  W+  ++  + +  LR +A  E+Q  +  L +A  +W+     + +   K + +  HY++ L  KV+ QW  V  +    RQ +   ++ +    D   L+  FQ WR    +   ++ +  +   H++W L    + +WK HH   + +  +  +  ++  +   R  F QW+K     K  +     A  +    ++ +VW  +  F+L+RR K +  E A Q++  +L+
Sbjct:  448 RRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWL--VYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLL 781          

HSP 2 Score: 54.6842 bits (130), Expect = 9.206e-7
Identity = 162/766 (21.15%), Postives = 300/766 (39.16%), Query Frame = 3
Query:  540 WYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRL--AITNWVAWIFVKKCKFKLRILA---EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWK---------QHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQE-KKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQ-LEHQRKINIDLASIHWQQKLQNKVFIVWR-MICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVI-QTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRG-------------------------WQA------QKSSLFWQKSELI------RIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYT 2672
            W +++W  + E +L  RA   ++     L+F  W  +   + E  +   +A   H D   ++  A  +W+ ++  ++ K  ++  A    +QSV   L F +  W+         +A  A++V H    L  +   +W     I ++ +     +  H+     + + + W +Y    ++K+ + E     +   +   +   W+           H  L+V+ A          R  LR+ F  W+ Y   +     Q++ A +  +  +    +  FK  + Q R + + K+ AQ   +  +L R+ + W SR+E++E       + AA SHYR        V+H K    W ++      +    + A   F+ R L  A  +W+   +  + +  L      F R  +  + +  WRQ+  + REN   +    ++ ++ Q   + +  W     V   E  Q      H +  LL + F    ++ Q    +R      A  H  Q L ++ + +WR  + LR + Q KL     H     ++R     W+ Y N+++ +  +V    ERQ N  ++    H WR     R    ++TS       R L  K    + ++W+  T V++   QK   A    R    R  L  W Q++     R                          W+A      +K  L  Q ++L+        +  WR+ LA R++E + T  AL  WA  L+AK++T W  + 
Sbjct:   23 WREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAE--HHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSV---LCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAG---------RMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVT------VLH-KCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQ-QLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLL-SRAWSMWRECLALRLEEQQKLKCAALHSQCILLRR-ALQKWLVYQNRVRSVLREVAAR-ERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSK----VLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFV 759          
BLAST of SFI1 vs. UniProt/SwissProt
Match: sp|A8K8P3|SFI1_HUMAN (Protein SFI1 homolog OS=Homo sapiens OX=9606 GN=SFI1 PE=1 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 1.285e-17
Identity = 154/717 (21.48%), Postives = 286/717 (39.89%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFH-RDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKEN---------AQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMIC--LRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQ 2483
            Y   +   R GRL+ELRIRC ARKF Y+W   + G+V    A  +Y +RL R  ++ W +++W  + E +L  RA   ++     L+F TW  + R + E  RN  +  + H      R A  +W+ ++ V++ K +++  A +   +I L+  +  W+          A  A ++ H    L  +   QW      V++ K   + +  H      +   + W EY    ++K+++ E  +  ++  +   Y   W+Q        YA   + +++ R+  LR+ F  W+ Y         Q ++A++ +          H + +   R  K+++            A       +L R+ + W S++E+K+       + AA  HYR         +  K    W Q+T     +    + A   F+ R L  A  +W+        ++ L      F R+ +  + ++ WRQ                             K F   + +L E + +     HA R+LL + +    Q     HQ +    +A  H +     K F +WR     LR +   ++    FH  +  + R  ++ W E        R+++       ++ ++ +   AW TY+   +   R+ + R  +  R    +L R    +WK  T  R+   +K+  A   YR     K L  W++  S+Q
Sbjct:   71 YRGTHTCTRQGRLRELRIRCVARKFLYLWIRMTFGRVFPSKARFYYEQRLLRKVFEEWKEEWWVFQHEWKLCVRADCHYRYYLYNLMFQTWKTYVRQQQE-MRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRIILRVWWSTWRQRLGQVRVSRALHASALKHRALSLQVQAWSQWREQLLYVQKEKQKVVSAVKHHQHWQKRRFLKAWLEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKMALRRAFTHWKHYMLLCAEEAAQFEMAEEHHR---------HSQLYFCFRALKDNVTHAHLQQIRRNLAHQQHGVTLLHRFWNLWRSQIEQKKERELLPLLHAAWDHYRI-------ALLCKCIELWLQYTQKRRYKQLLQARADGHFQQRALPAAFHTWNRLWRWRHQENVLSARATRFHRETLEKQVFSLWRQ-----------------------------KMFQHRENRLAERMAI----LHAERQLLYRSWFMWHQQAAARHQEQEWQTVACAHHRHGRLKKAFCLWRESAQGLRTERTGRVRAAEFHMAQ--LLRWAWSQWRECLALRGAERQKLMRADLHHQHSVLHRALQAWVTYQGRVRSILREVAARESQHNR----QLLRGALRRWKENTMARVDEAKKTFQASTHYRRTICSKVLVQWREAVSVQ 731          
BLAST of SFI1 vs. UniProt/SwissProt
Match: sp|A9CB34|SFI1_PAPAN (Protein SFI1 homolog OS=Papio anubis OX=9555 GN=SFI1 PE=3 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 1.455e-17
Identity = 159/744 (21.37%), Postives = 291/744 (39.11%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYY---GEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKEN---------AQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKI-QKRNKIFHK----WFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQ-LEHQRKINIDLASIHWQQKLQNKVFIVWR-MICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELI 2543
            Y   +   R GRL+ELRIRC ARKF Y+W   + G+V    A  +Y +RL R  ++ W +++W    E +L  RA   ++     L+F TW  + R + E       A         R A  +W+ ++ V++ K +++  A +   +  L+  + +W+          A  A +V H    L  +   QW      V++ K   + +  H      +   + W EY    ++K+++ E  +  ++ ++   +   W+Q        YA   + +++ R+  LR+ F  W+ Y     EE     Q ++A++ +          H + +   R  K+++            A       +L R+ + W S++EEK+       + AA  HYR         +  K    W Q+T     + +  + A   F+ R L  A  +W       +  + L      F R+ +  + ++ WRQ     +  + Q N+  E   I     ++ H+    W      +  E         H     L K F    ++ Q L  +R   +  A  H  Q L  + +  WR  + +R   + KL+    H  + R++      W+ Y  +++ I ++V                 A R  +H RQ                    L R    +WK  T  R+   +K+  A   YR     K L  W++  S+Q    ++ Q+ S FW+  +++
Sbjct:   64 YRGTHTCTRQGRLRELRIRCVARKFLYLWIRMTFGRVFPSKARFYYEQRLLRKVFEEWKEEWWVFHHEWKLCVRADCHYRYYLYNLMFQTWKTYVRQQQEMRNKYTRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTTALEFRQRSILRVWWSMWRQQLGQVRVSRALHASAVKHRALSLQLQAWSQWREQLLYVQKEKQKVVSAVKHHRHWQKQRFLKAWLEYLQVRRVKRQQNEMAERFHHVSVLQIHFCDWQQAWEQRQSLYAYHAQVEKLARKMALRRTFTHWKHYMLLCAEEA---AQCEMAEEHHR---------HSQLYFCFRALKDNVTHAHLQQIRRNLAHRQHGVTLLHRFWNLWQSQIEEKKEREQLPLLHAAWDHYRI-------ALLCKCIKLWLQYTQKRRYKQRLQARADGHFQQRTLPAAFHTWKRLWRWRQQDNVLNARATRFHRETLEKQVFSIWRQ-----KTFQHQENRLAERMAILHAERQLLHRSWFTWHQQAAARHQEQEWQTAACAHHRHGRLKKAFCLWRESAQGLRAERTGRVRAAEFHVAQLL-RRAWSQWRECLAVRGAERRKLMRADRHHQQ-RVRHRALQAWVTYQGRVRSILQEV-----------------AARESQHNRQ--------------------LLRGALRRWKENTMARVDEAKKTFQASAHYRRTICSKVLVQWREAVSVQIY--YRQQEDSAFWEARKVL 742          
BLAST of SFI1 vs. UniProt/SwissProt
Match: sp|Q3UZY0|SFI1_MOUSE (Protein SFI1 homolog OS=Mus musculus OX=10090 GN=Sfi1 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 7.905e-15
Identity = 177/811 (21.82%), Postives = 329/811 (40.57%), Query Frame = 3
Query:  402 RLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRL--AITNWVAWIFVKKCKFKLRILA---EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWK---------QHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQE-KKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWR-MICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVI-QTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRG-------------------------WQA------QKSSLFWQKSELI------RIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYT 2672
            RL+ELRIRC ARKF Y+W   + G+VT   A   + +++ +  +  W +++W  + E +L  RA   ++     L+F  W  +   + E  +   +A   H D   ++  A  +W+ ++  ++ K  ++  A    +QSV   L F +  W+         +A  A++V H    L  +   +W     I ++ +     +  H+     + + + W +Y    ++K+ + E     +   +   +   W+           H  L+V+ A          R  LR+ F  W+ Y   +     Q++ A +  +  +    +  FK  + Q R + + K+ AQ   +  +L R+ + W SR+E++E       + AA SHYR        V+H K    W ++      +    + A   F+ R L  A  +W+   +  + +  L      F R  +  + +  WRQ+  + REN   +    ++ ++ Q   + +  W     V   E  Q      H +  LL + F    ++ Q     ++    A+     +L ++ + +WR  + LR + Q KL     H     ++R +   W+ Y N+++ +  +V    ERQ N  ++    H WR     R    ++TS       R L  K    + ++W+  T V++   QK   A    R    R  L  W Q++     R                          W+A      +K  L  Q ++L+        +  WR+ LA R++E + T  AL  WA  L+AK++T W  + 
Sbjct:   58 RLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAE--HHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSV---LCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAG---------RMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVT------VLH-KCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQ-QLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQK-WLVYQNRVRSVLREVAAR-ERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSK----VLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFV 840          

HSP 2 Score: 65.4698 bits (158), Expect = 3.443e-9
Identity = 74/336 (22.02%), Postives = 153/336 (45.54%), Query Frame = 3
Query:  435 RKFFYIWRNKS----IGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILA--EKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPI---VRQSKLNRMK-SNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENA-QSFANKLI 1409
            R+F+++W  ++    + +  + +A  H+   L R  + IW +     RIE     +A  FH +  +   ++ W +   LRLE  + ++ A    +  + R A+  W+  ++  + +  LR +A  E+Q  +  L +A  +W+     + +   K + +  HY++ L  KV+ QW  V  +    RQ +   ++ +    D   L+  FQ WR    +   ++ +  +   H++W L    + +WK HH   + +  +  +  ++  +   R  F QW+K     K  +     A  +    ++ +VW  +  F+L+RR K +  E A Q++  +L+
Sbjct:  529 RRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWL--VYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLL 862          
BLAST of SFI1 vs. UniProt/SwissProt
Match: sp|B0KWR6|SFI1_CALJA (Protein SFI1 homolog OS=Callithrix jacchus OX=9483 GN=SFI1 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.037e-10
Identity = 148/722 (20.50%), Postives = 283/722 (39.20%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQW---LCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKL--------ILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHF----VRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMIC--LRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQ 2483
            Y   +   R GRL+EL IRC ARKF Y+W   + G+V    A  +Y ++L R  +  W +++W    E +L  RA   ++     L+F TW  +   + E       A         R A  +W+ ++  ++ K +++ +A +   +  L+  + +W+          A  A +V H +  L  +   QW   L      +Q   + +K   H+     +   + W  Y    ++K+++ E  +  ++  +   +   W+Q        +A   + +++ R+  LR  F  W+ Y         Q ++A++ +     RR++  F++      + N    + Q     L        +L R+ + W S++E+K+       + AA  H+R         +  K    W Q+T     +    + A   F+ R L  A  +W+      + ++ L     HF    V K V   +W K  Q    REN            ++ +R  I                       HA R+LL + +    Q     HQ +    +A  + + +   K F +WR     LR +   ++    F+  +  + R  ++ W E        R ++       ++ ++++   AW TY+   Q   ++ + +  +  R    +L R +  +WK  T  R+   +K+  A   YR     K L  W++  S+Q
Sbjct:   64 YRGTHACTRQGRLRELHIRCVARKFLYLWIRMTFGRVFPSKARFYYEQQLLRKIFGEWKEEWWVFHHEWKLCVRADCHYRYYLYNLMFQTWKTYVHQQQEMRSKYIRAEVHDAKQKMRQAWKSWLIYVVFRRTKLQMQTMALEFRQRSILRVWWSMWRQRLGQIRVSRALHASAVKHRSLSLQLQAWSQWWEQLLYAQKEKQKVASAVKHRQHWQK---RRFLKAWLAYLQIRRVKRQQNEMAERLHHVTMLQIHFCNWQQAWERRESLHAHQAQVEKLARKMALRCTFTHWKHYMLLCAEEAAQCEMAEEHHR---HRRLYFCFRAL-----KDNVAYTHLQQIRRNLAYQQRGITLLHRFWNLWWSQIEKKKEREQLPLLHAAWDHHRM-------ALLCKCVKWWLQYTQKRRYKQLLQARADGHFQQRALPAAFHTWNRLWRSHQQENVLSARATHFHREKVEKQVFSIWWQKMVQH---REN------------RLAERMAIL----------------------HAERQLLHRSWFTWHQQAAAHHQEQEWQTVARAYHRCRRLRKAFCLWRESARGLRTERMGRVQAAEFNAAQ--LLRWAWSQWRECLALRGAERRKLMRAELHHQHTVLLRALQAWVTYQGRVQSILQEVAAKESQHNR----QLLRGVLRRWKENTMARMNEAKKTFQASTHYRKTICSKVLVQWREIASVQ 724          
BLAST of SFI1 vs. TrEMBL
Match: H6WA41 (SFI1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 1377.84 bits (3565), Expect = 0.000e+0
Identity = 682/689 (98.98%), Postives = 682/689 (98.98%), Query Frame = 3
Query:  399 GRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWK 2465
            GRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR       RVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWK
Sbjct:    1 GRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR-------RVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWK 682          
BLAST of SFI1 vs. TrEMBL
Match: A0A4D9DTT6 (SH3 domain-binding protein 5-like OS=Platysternon megacephalum OX=55544 GN=DR999_PMT16710 PE=4 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 6.725e-29
Identity = 218/921 (23.67%), Postives = 376/921 (40.83%), Query Frame = 3
Query:  360 LKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQW-------REYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKL--------ILRRYTDKWISRLEEKENVNTKTEMGAARSHYR------------------------FPKRDSE--RVMHMKYFSKWSQFTSSAAVRN-----QNLSNAIKMFETRLLMNAI-DSWHSFVIISKAKSRLINLKEHFV----RKYVLCKYWTKWRQEK----KARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQ------NVQLEHQRK--INIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYR---IKRIVFNCWIEYTNQM-KQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYY------SIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATR 2903
            L PY   Y WNRGGRLKELRIR  ARKF Y+W  K+ GQV    A  HY R++ + T+  W +++W +  E +L  RA   ++     L+F  W  +   + E+     VA         R    +W+ +I V++ K ++R  A        L+ ++ +W    + K        +++ H+ Q L  +   QW+ +   ++  K    ++ NH     L+   + W       RE  H+ K+ Q+       H+   +  +    W++   H        +   E+  R  LR+ F  W+ Y     VL +++    ++ E   +     H   F      KN I  + Q     L        +L+ + ++W  RLE++E  + ++   AAR+HYR                        + K DS   RV+    F  W +F      +N     + +      F   LLM  + D+W     +S  + R    +E       ++ VL ++W  WR+      + RE+  +  + Y  +       K FH W         +NIQ  R +     K +   ++  LQ         + H+ K    +  A +++Q  L  +V   W+     +++   +L     K E R   + R V + W E T  +  + ++ VQ     +R+ +  +    WR     R Y ++Q +  L + +R LD+   R+ F+ WK  T   L+   +   A   ++   LR+ L  WKQY+       + Q RG Q     L          +  W++ LA +Q E   T  AL HW+  L+ K+F  W  +  ER+ K  +  +A + Y   L K  + Q +T    + Q+R Q   +H  ++ ++    +  +V R    WKR    R
Sbjct:   47 LVPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGQVLPSKARCHYDRKILQKTFGEWKEEWWIVCREWKLSIRADCHYRYFLYNLIFQAWKTFICQQREKRSKYRVAEAHAEKQKLRWTCQHWLIYIDVRRTKHRMRSEALAFRETSTLRVSWRVWTRRLRQKCTGREMNTLALQHWAQSLQFRAWLQWMELYLHIQHDKQKEARAVNHHQHWELRRCMKAWLGHLQLQREKKHQDKLAQQ-------HHQVCVLQQCFSGWRRAWEHRRSMRIHQECIGELAARVTLRRGFTHWKHYV----VLCMEETWKHKLAEKHHRH----HLLHFGFNALRKNIINAHLQQMRRNLAHRQHQVMLLQSFWNRWKCRLEQEEEEHQRSLTLAARNHYRVTLLQKSVRLWLRNAKWRRHRQMQYAKADSHYGRVILPVTFQAWKRF------KNYQRWWREMKETASCFHRELLMRQVFDTW----WLSACQHRENRTRERMAILHSKQQVLVRFWCSWRRRTVVYLEERESTALAHDHYCHL----LLRKTFHFW--------KKNIQEIRSERIKEVKAIRFHYSKCLQWSWSKWRKYVGHRSKKWKTLVRADMYYQHSLLARVLAAWKSY---QQNIQCILRQVDEKDEERNRALLRQVLSIWRENTVALVGEAKKAVQAELHYRRSTLS-KVLLQWRDTTCLRVYCRQQEAAALRQARRCLDMVHLRATFLHWKEFTTRSLVLRVQLDTAAQHHQRQLLRECLAKWKQYHLQCIGKMLLQRRGDQLMAQRL------CSTCFSCWKRQLAQKQWERQETVRALWHWSLSLQGKVFDAWLGFALERQRKKGRIEKAVAVYHAALLKEGVTQILTYMAGMKQFRGQLQAQHQLKAAYS----LHQTVYRCAMLWKRKALCR 916          
BLAST of SFI1 vs. TrEMBL
Match: A0A401SX69 (Uncharacterized protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0013413 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.749e-28
Identity = 206/925 (22.27%), Postives = 385/925 (41.62%), Query Frame = 3
Query:  381 YKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKW-TRLRLERSRNMEVARKF-HRDN-IGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKM-AISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEY---AMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR--------------------FPKRDSERVMHMK------YFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEK----KARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLI-WRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSN----STNRKTVETFPLELKQSSRK---LFQKQIVSQQPKIR 3020
            Y WNRGGRLKELR+R  ARKF ++W  K+ G++    A  HY     R T+  W +Q+W +R+E +L  RA   ++     +   TW  W T +  ++ +  ++A+   H D  + R    NW  ++ ++K K  ++  A++  VK  L+ A+ IW    + K E N +M  +++ H+ + L  +   QW  +  + +Q +    ++   +    L+     W  Y    + K+ +       Y  ++  +Y + W   HA   ++    A  D  +E+ RR  LR+ FI+W  Y           +LAD      + +      +  +   R+K  +   A    +  +LRR  ++W  RL+++E +  +   G A++H+R                    +  +D +   H        Y  +W  F S     NQ    A +     LL  A  +W   +   +       L      + VL ++W  WR++     + RE   I  + +    K Q   K  H W      ++       R   H  R  L + + A    VQ +H++      A +H+Q+KL +K    W++     +     +  +  K    + R  F  W E    +     +     +   + ++ +    WR     + Y+++Q +  + E ++++D    +  F  WK  +   ++   K K A   Y    + K + +WKQ+++   LR    Q+   ++Q   L R +   W+  L  + +E   T  AL HW+  L+ K+F  W  Y  E++ K  +  +A   Y++ L +  +   +     ++Q+R+Q A +H  ++ ++  Q     V R    WK+    +  RS     +  RK    F L + +  ++     +  I+  +PK R
Sbjct:  113 YTWNRGGRLKELRLRHLARKFLHLWIWKTFGRILPSQARSHYCHATLRKTFHEWKEQWWSVRVEWKLMVRADCHYR---YHMYNQTWRAWHTYILQQQMKKTKLAKAISHADTQMFRKFWINWTLYVKIRKTKRMMQFEADEFRVKATLQAAWFIWTKQLERK-ERNRRMDGLAMQHWAKTLQHRAWLQWNALLHLSQQERETDGRAQQIYQRRCLQRCLLAWLVYVQVCRDKRCQYRLARQTYERSVVQQYFLSW---HAAWCLQRSIRAQDDHLRELGRRNILRRTFIRWSHYVSLAAEKTNLCRLADDHYHHHLLQSSVTALRFNVRSVRQKQMLNNLAHQQFHVWMLRRCWNQWKLRLDQQEELELRALTGQAQNHFREVLLRKSFHCWIKGIQEEKWYQVQDKKAHAHYCQNLLPLYLKRWKVFASEKKRLNQMKETAWEFHREMLLKLAFHTWWEKLDRQRENRTAERLAVIHYTRCVLLRFWWNWREKTVILLEEREKEAIAEDHF----KHQLLRKSLHFWKKTVAEQIAGRDHEVRAVRHWWRCRLRRTWRAWRLYVQKKHEKWKKHVCADVHFQKKLLSKALREWKVFHCNNQQILCKVDEKEKKQRRDLLRFSFYTWRENARTLADEARKAARADQHYHHVLLAKAVEYWRDAIALQMYQRQQEAELVLEARQRIDFLQLQHAFSHWKQISRSSVILRDKMKAAAQHYGQKIMMKCMLSWKQHHAYY-LRAVLLQRRGDWFQALRLYRCYFTNWKVKLLLKHQETKKTAVALWHWSLSLQGKVFDAWLMYIEEQRRKKLRIAKAVQTYRSHLLRLGVAAILQYTSDMVQFRSQVAAEHQMKTAYSLHQV----VYRCAMTWKQKALCKRERSKHKSCAVTRKKSVAFRLPVSEVCKENSVSMKTGILKSEPKER 1021          
BLAST of SFI1 vs. TrEMBL
Match: A0A1S3HJD4 (protein SFI1 homolog OS=Lingula unguis OX=7574 GN=LOC106155706 PE=4 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 4.425e-27
Identity = 201/891 (22.56%), Postives = 370/891 (41.53%), Query Frame = 3
Query:  354 KALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAW---IFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKW-KQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFT---------------------------------ALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLD-VKLKRSIFIKWKAQTHVRLLAIQKSKIADDL-YRANCLR-KYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELI-RIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATR 2903
            K  K + P Y WN+GGRLKE+RIR  ARK+ YIW  K+ G+V   +A +H+  ++ +  +  WY+ +W  R E RL  RA   ++     +VF  W ++T   L++ +N + A      N+ R+    W AW   I +++ K  L   A   + +  L+  +  W +AF  + E       ++  + + L  +   +W       +Q       +  H D  +L   ++ W  +    + K+ +K+     Y  NL  R    W K+ HA  LV   + +R + +R R + R+    W+ Y    +  + +++ AD+     +        +  ++Q R +   K  A    N+++L++  +KWI+  ++ E +   T    A +HY+         +    F KW  +      R    + A   +  RL+   +  W  FV + K K  + N  + F R+ +L +++  W +  K  + + +     +         +   KW   TK +  +  +     +H    L+S+ F                                     + +  E ++K  +  A +   QK+  KV   W+  C R     + +  R    +  ++R     W     + KQ ++Q     +   +  + + F AWR Y +   Y  ++T+ +L   Q+ L  VKL   I  +W    +  +  IQ+ K    + +  N LR + +  WK Y  + Q R    QK  ++   + L  + +L WR   A+ Q +H     AL  W+  L+ K+F  W  YT+ER  K  +   A  Q + RL +    QW+     L + R++ A +  +Q+ +   Q V    +     W+++T  R
Sbjct:  136 KESKRHRPDYTWNKGGRLKEIRIRHLARKYLYIWIRKTFGRVLPSVARKHHNTQVLKQAFAEWYELWWVARKEWRLQVRAECHNRYRVWNVVFKAWTRYT--VLQKVKNAKKAMAERHGNV-RVLSRAWQAWRLYIRIRRTKKGLSTRARVFATENLLRSCWIRWVMAFDKRQEQKDVEVFALQIWAEKLQAQCWLKWKAAFKARQQVYFKSRVAEKHRDVRLLSRCWKGWLLHVIIRRRKKTQKDVAMMMYQNNLKQRLFRIWCKRCHAARLVA-QVQERIEILRNRARARRTLEHWKFYINLRQKKKEKEQQADEHYRKHLMSSCLSALQLNVVQERLQVMRKRMADQCYNQMLLQKTWNKWIACCDQNEELKLYTVNKKAWTHYKM-------TLLGTSFEKWVNYNIWRQHRKAQYARADAHYINRLMPMCVFRWRQFVNLMKQKREMENDGKEFRRENLLARFFYTWMERCKQSQEVRLMERMAILHDSEMTTRRFLLKWRNATKERQVDMKKEENAHDHYQITLMSQAFETWKTFIRDSRKSYESEKKAVRYHYLKTMGVTWKGWRKYIGYERRKKTKLHRAVVFHNQKICGKVIGHWKEYCRRAAVINEEVERRAQIHDKELQRRCLIQWHRNAVEQKQNKQQEAKADKFHGHIALKKVFSAWREYANVHAYEHKKTAEKLLSAQQHLGKVKL-HYILTRWYQLKNGAV--IQRLKFQQAVEHHNNTLRMRTITVWKNYTVLCQ-RKLLLQKQCMWLYTTRLTAKFFLKWRAQFAEAQEDHTKNNMALWQWSFVLQRKVFLAWQTYTHERHRKKARIELAMEQRRLRLIRQGCGQWLKVATELSEVRSKLAAERQAQTAYNIYQLVHKCALH----WRQWTIKR 1007          
BLAST of SFI1 vs. TrEMBL
Match: A0A1S3IS48 (protein SFI1 homolog OS=Lingula unguis OX=7574 GN=LOC106166910 PE=4 SV=2)

HSP 1 Score: 130.954 bits (328), Expect = 9.932e-27
Identity = 199/915 (21.75%), Postives = 378/915 (41.31%), Query Frame = 3
Query:  354 KALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAW---IFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKW-KQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFT---------------------------------ALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLD-VKLKRSIFIKW---KAQTHVRLLAIQKSKIADDLYRANCLR-KYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSS 2972
            K  K + P Y WN+GGRLKE+RIR  ARK+ YIW  K+ G+V   +A +H+  ++ +  +  WY+ +W  R E RL  RA   ++     +VF  W ++T   L++ +N + A      N+ R+    W AW   I +++ K  L   A   + +  L+  +  W +AF  + E       ++  + + L  +   +W       +Q       +  H D  +L   ++ W  +    + K+ +K+     Y  NL  R    W K+ HA  LV   + +R + +  R + R+    W+ Y    +  + +++ AD+     +        +  ++Q R +   K  A    N+++L++  +KWI+  ++ E +   T    A +HY+         +    F KW  +      R    + A   +  RL+   +  W  FV + K K  + N  + F R+ +L +++  W +  K  + + +     +         +   KW   TK +  E  +     +H    L+S+ F                                     + +  E ++K  +  A +   QK+  KV   W+  C R     + +  R    +  ++R     W     + KQ ++Q     +   +  + + F AWR Y +   Y  ++T+ +    Q+ L  VKL + I  +W   K +  ++ L  Q++      +  N LR + +  WK Y  + Q +    ++    +      + +L WR   A+ Q +H     AL  W+  L+ K+F  W  YT+ER+ K  +   A  Q + RL +    QW+     L + R++ A +  +Q+ +   Q V    +     W+++T     R  +  ++ + + P+   +SS
Sbjct:  136 KESKRHRPDYTWNKGGRLKEIRIRHLARKYLYIWIRKTFGRVLPSVARKHHNTQVLKHAFAEWYELWWVARKEWRLQVRAECHNRYRVWNVVFKAWTRYT--VLQKVKNAKKAMAERHGNV-RVLSRAWQAWRLYIRIRRTKKGLSTRARAFATENLLRSCWIRWVMAFDKRQEQKDVEVFALQIWAEKLQAQCWLKWKAAFKARQQVYFKSRVAEKHRDVRLLSRCWKGWLLHVIIRRRKKTQKDVAMMMYQNNLKQRLFRIWCKRCHAARLVA-QVQERIEILGNRARARRTLEHWKFYINLRQKKKEKEQQADEHYRKHLMSSCLSALQLNVVQERLQVMRKRMADQCYNRMLLQKTWNKWIACCDQNEELKLYTVNKKAWTHYKM-------TLLGTSFEKWVNYNIWRQHRKAQYARADAHYINRLMPMCVFRWRQFVNLMKQKREMENDGKEFRRENLLARFFYTWMERCKQSQEVRLMERMAILHDSEMTTRRFLLKWRNATKERQVEMKKEENAHDHYQITLMSQAFETWKTFIRDSRKSYESEKKAVRYHYLKTMGVAWKGWRKYIGYERRKKTKLHRAVVFHNQKICGKVIGHWKEYCRRAAVINEEVERRAQIHDKELQRRCLIQWHRNAVEQKQNKQQEAKADKFHGHIALKKVFSAWREYANVHAYEHKKTAEKFLSAQQHLGKVKLHQ-ILTRWYQLKNEAVIQRLKFQQAV----EHHNNTLRMRTMTVWKNYTVLCQKKLLLQKQCMWLYTTRLTAKFFLRWRAQFAEAQEDHTKNNMALWQWSFVLQRKVFLAWQTYTHERQRKKARIELAMEQRRLRLIRQGCGQWLKVATELSEVRSKLAAERQAQTAYNIYQLVHKCALH----WRQWT---IKRKAARLKRGITSAPIPGGESS 1027          
BLAST of SFI1 vs. Ensembl Cavefish
Match: sfi1 (SFI1 centrin binding protein [Source:NCBI gene;Acc:103043034])

HSP 1 Score: 76.6406 bits (187), Expect = 1.495e-13
Identity = 175/862 (20.30%), Postives = 318/862 (36.89%), Query Frame = 3
Query:  369 YDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNH--FDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQ---EMRRRFQLRKYFIQWQKY--YGEEKVLRVQQKLADQ---MNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFV----IISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFH-----------KWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKH----ERQYRKR-----------------------------QTSYRLDEGQRKLDVKLKRSIFIKWKA-QTHVR--LLAIQKSKIADDLYRANCLRKYLDAW------KQYYSIQQLRG---------------WQAQKSSL------------FWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYT 2672
            Y   Y WNRGGRLKELRIR  ARKF  +W  K+ G+VT   A  +Y + + +     W  ++W  R E  L  RA   +  V    V+  W ++   + E  R +++A  F      R     W  ++ +++ K ++   A K      ++  + +W++A Q +     +  +++ H+   L  +   +W   R      +L   +S  H  +   + K     W  +    + K + K    + +  ++  R+ + W++    L+   A  DR Q    + +R   R  F  W+ Y     EK  + Q  L  Q   +  + +K          + Q +   + K  +       + R+Y   W  RLE  E  + + +M  A +++RF       V+ + Y  +W Q         +    A   F  R+L  ++ SW  F        K + R     +H V  +    +W +   ++                Q++ +R  + H           KW      +  + ++  + Q      +L K      QNV+     +     A ++  Q+        WR     ++ +T+ L       E R+ R     W ++  Q +QI + V I +++ +  +  +    WR   H    ER+  +R                             Q    L E Q  +D     ++  +WK  Q  VR   L+++K   A   + +  LR+ L AW       + Y + + R                W+AQ  S              W  +   ++  +WR  +A+RQR+      A   + + L  +  TH   YT
Sbjct:   49 YRVGYTWNRGGRLKELRIRHLARKFLRLWIQKTFGRVTTSQARSYYRKVILKKVLSAWKDEWWYARKEWTLTIRADCHYNYVQYSKVYQAWREYVSDQSEEKRRLQIATIFMERRRLRAVWDGWELYVEMRRLKRRMHETAIKHQRLATVRRVWTVWQVAVQQRYAQYHQEDLAMQHWATNLQSRSWQRW--KRRCSEACRLREEESRAHLHYSHRLQKCVLHSWITHTEHRQAKNQTKAISGNLWCRSVIRRHWVVWRRA---LMCRQAERDRGQIADSLAQRITQRWAFSCWRDYIQLCSEKADKKQTALQHQQLRLQHVGLK-----GLALNVTQCKTHRTNKNISAQHHQHTMKRKYWKFWQERLEMIEERSFQPQMTQALNYHRFS------VLKI-YLQRWRQRCKEHKRMKELQLRADSCFAKRVLPQSMASWIEFTEQRTDHRKRRERAELFHQHQVYSWAFYTWWGRCVDQRD---------------QRLAERTAVLHAEQVCVSHAWSKWLCRVLQQREDRLKQAQAQKLHTHTVLRKTLHQWSQNVKAIQNSQRQFVQAKVYHDQRCMRGALNCWREYVAHRRQRTQRLALIDEHYERRLLRNTLQSWKQHYVQTQQINQCVNIRYQQHQQDLARRVLSIWRRNVHLAVDEREKERRANCHYQHCLLSKVLSAWHQRTTHIIAHHHQQEEALREAQLHMDKVRMLAVLRRWKQRQVVVREERLSLEK---ACRHHSSVLLRQCLTAWIISNFKHKSYQVMKNRSRELHRLKVCRHFFICWKAQLQSRRREAELTVMALWHWSLNLQAKVLCVWRHWVAERQRKQRRLAVAAQVYRDELMREGVTHILTYT 875          
BLAST of SFI1 vs. Ensembl Nematostella
Match: EDO36895 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHD3])

HSP 1 Score: 59.6918 bits (143), Expect = 6.873e-9
Identity = 99/472 (20.97%), Postives = 189/472 (40.04%), Query Frame = 3
Query:  114 KRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPM-KTHNVIPLSXXXXXXXXXXXXXSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSH-YNWNLAHRY---LMKWKQHHAHLLVEYAMADR--CQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRF 1508
            K+++L+ LN HL   AIR + ++++ +    + P      +K   K  + +   + KT  ++                   + +K     Y W+  GR  E RIR  ARK+  +W+  + GQ+T  LA   Y+        Q ++  ++    +  L    +   K    +  F  W  +  L   +   +++A ++    +    I  W  +I  ++ K     +AE+ S  + ++ A+  W     +K E++     ++  +   L+QK    W       ++ K     +  +F+  ++   +  W+ Y    ++   +K   D H YN  L   +     +W + +     E  ++ R     MRR F   K+ I     Y   K  + +   A    + L+   + C F+  +++RR K    + A+    KL LRR    W+ R E  E +        AR+HYRF
Sbjct:   53 KKAVLQELNTHL---AIRAQGLQLKLHDDRGENPYSVGKIVKDKRKPPVAKHKTVRKTEEIVTDEVVQ-------------EKVKAKPSGYDWDMHGRQAEHRIRYLARKYLMLWQKHTYGQITPSLA--RYI--------QNFFITFFCYSKDTLLAVYKVSSEKYRIWQRTFKAWKLFVMLSRAKKTKIKLASQYAEGKLMETCIKAWKQYISSRRFKTVKCKIAERHSNTLIMRRAWQQWGHQMSIKYELSIMETQAMQFWAWNLMQKGWSVWAAAYCTRQKEKSKLAAAEGYFNKRLMSHTWVAWKSYRASKRLSNLQKRVADRHFYNRTLQQAFKAWTSRWHRQNELAQFEEIISSRGATASMRRSFIHWKFCILVGISYMSLKYEKEELATA-HYRKHLLSTGLSC-FQLQVVERRLKIMRNKMAKEACYKLTLRRAWIVWLKRCEHSEELKLYVLTRTARTHYRF 496          
BLAST of SFI1 vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 2281.91 bits (5912), Expect = 0.000e+0
Identity = 1147/1147 (100.00%), Postives = 1147/1147 (100.00%), Query Frame = 3
Query:   90 MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSXXXXXXXXXXXXXSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 3530
            MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR
Sbjct:    1 MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 1147          
BLAST of SFI1 vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 2278.83 bits (5904), Expect = 0.000e+0
Identity = 1140/1147 (99.39%), Postives = 1140/1147 (99.39%), Query Frame = 3
Query:   90 MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSXXXXXXXXXXXXXSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 3530
            MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR       RVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR
Sbjct:    1 MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR-------RVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 1140          
BLAST of SFI1 vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 2278.44 bits (5903), Expect = 0.000e+0
Identity = 1140/1147 (99.39%), Postives = 1140/1147 (99.39%), Query Frame = 3
Query:   90 MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSXXXXXXXXXXXXXSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 3530
            MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR       RVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR
Sbjct:    1 MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR-------RVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 1140          
BLAST of SFI1 vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 2258.41 bits (5851), Expect = 0.000e+0
Identity = 1140/1147 (99.39%), Postives = 1140/1147 (99.39%), Query Frame = 3
Query:   90 MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSXXXXXXXXXXXXXSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 3530
            MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR       RVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR
Sbjct:    1 MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYR-------RVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 1140          
BLAST of SFI1 vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 2239.15 bits (5801), Expect = 0.000e+0
Identity = 1126/1126 (100.00%), Postives = 1126/1126 (100.00%), Query Frame = 3
Query:  153 MSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSXXXXXXXXXXXXXSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTXXXXXXXXXXXXXFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 3530
            MSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR
Sbjct:    1 MSAIRLKNIEIETNILPEKKPLITKIQIKPSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKWNRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQIWYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEVARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIWKIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKSNNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWKQHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLADQMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWISRLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVRNQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLCKYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNENIQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNKVFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIREQVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKLKRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQLRGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLEAKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQWRNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTVETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAGNIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNETNQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSFYADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR 1126          
The following BLAST results are available for this feature:
BLAST of SFI1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SFI11.233e-2022.44SFI1 centrin binding protein [Source:HGNC Symbol;A... [more]
SFI12.675e-1821.48SFI1 centrin binding protein [Source:HGNC Symbol;A... [more]
SFI11.397e-1121.33SFI1 centrin binding protein [Source:HGNC Symbol;A... [more]
SFI11.255e-1030.19SFI1 centrin binding protein [Source:HGNC Symbol;A... [more]
SFI11.140e-720.12SFI1 centrin binding protein [Source:HGNC Symbol;A... [more]
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BLAST of SFI1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SFI1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SFI1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
sfi14.381e-1820.72SFI1 centrin binding protein [Source:ZFIN;Acc:ZDB-... [more]
sfi16.636e-1820.72SFI1 centrin binding protein [Source:ZFIN;Acc:ZDB-... [more]
sfi16.669e-1720.82SFI1 centrin binding protein [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of SFI1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
SFI18.028e-1827.27SFI1 centrin binding protein [Source:NCBI gene;Acc... [more]
SFI13.079e-1730.00SFI1 centrin binding protein [Source:NCBI gene;Acc... [more]
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BLAST of SFI1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Sfi11.130e-1521.82Sfi1 homolog, spindle assembly associated (yeast) ... [more]
Sfi11.246e-1521.82Sfi1 homolog, spindle assembly associated (yeast) ... [more]
Sfi15.396e-1522.06Sfi1 homolog, spindle assembly associated (yeast) ... [more]
Sfi15.232e-1321.69Sfi1 homolog, spindle assembly associated (yeast) ... [more]
Sfi15.697e-1022.02Sfi1 homolog, spindle assembly associated (yeast) ... [more]
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BLAST of SFI1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|A8K8P3|SFI1_HUMAN1.285e-1721.48Protein SFI1 homolog OS=Homo sapiens OX=9606 GN=SF... [more]
sp|A9CB34|SFI1_PAPAN1.455e-1721.37Protein SFI1 homolog OS=Papio anubis OX=9555 GN=SF... [more]
sp|Q3UZY0|SFI1_MOUSE7.905e-1521.82Protein SFI1 homolog OS=Mus musculus OX=10090 GN=S... [more]
sp|B0KWR6|SFI1_CALJA1.037e-1020.50Protein SFI1 homolog OS=Callithrix jacchus OX=9483... [more]
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BLAST of SFI1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H6WA410.000e+098.98SFI1 (Fragment) OS=Schmidtea mediterranea OX=79327... [more]
A0A4D9DTT66.725e-2923.67SH3 domain-binding protein 5-like OS=Platysternon ... [more]
A0A401SX691.749e-2822.27Uncharacterized protein OS=Chiloscyllium punctatum... [more]
A0A1S3HJD44.425e-2722.56protein SFI1 homolog OS=Lingula unguis OX=7574 GN=... [more]
A0A1S3IS489.932e-2721.75protein SFI1 homolog OS=Lingula unguis OX=7574 GN=... [more]
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BLAST of SFI1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sfi11.495e-1320.30SFI1 centrin binding protein [Source:NCBI gene;Acc... [more]
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BLAST of SFI1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SFI1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SFI1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO368956.873e-920.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SFI1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SFI1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000002829.10.000e+0100.00SMESG000002829.1[more]
SMESG000002829.10.000e+099.39SMESG000002829.1[more]
SMESG000002829.10.000e+099.39SMESG000002829.1[more]
SMESG000002829.10.000e+099.39SMESG000002829.1[more]
SMESG000002829.10.000e+0100.00SMESG000002829.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30017201
GenBankAFB74723.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017201 ID=SMED30017201|Name=SFI1|organism=Schmidtea mediterranea sexual|type=transcript|length=3564bp
GTAAAGTTACGGCGTTCATTTAAATTAATAACTTTTTATATTTTAAAAAA
AAGTTTCTAAAGATTTGAATTAAGATTTCTAAATTTCTAATGACCCAAAA
GTTAGAACATAAAAAACGTTCTATACTAGAGAGTTTAAACGGACATCTTC
AAATGTCAGCTATTAGGTTAAAGAATATTGAAATTGAAACAAATATATTA
CCAGAAAAAAAGCCTCTTATAACAAAAATTCAAATTAAGCCATCAAATAA
AATGGCAATAACTCGGTTAGATCCAATGAAAACTCATAATGTTATTCCTT
TATCAAAAAATCCAATTATTAAGCCCAATAATAAACCAAATAAATCAACA
TTCAAAGCATTAAAACCTTATGATCCTTTGTATAAATGGAATCGTGGAGG
TCGGCTAAAGGAATTAAGAATTCGTTGCCAAGCTCGAAAATTCTTTTACA
TTTGGCGTAATAAATCAATTGGTCAAGTCACTGAATTGCTTGCAACACAG
CATTATCTACGGCGTTTAAAGCGGACGACATGGCAGATTTGGTACCAACA
ATATTGGCGATTGAGAATTGAGCCGCGTCTGTTCGGTCGAGCTATTTTAT
TTCATAAATCAGTATCCATACGTCTCGTATTTACGACATGGATCAAATGG
ACGAGATTACGGTTGGAAAGAAGCAGAAATATGGAAGTGGCGAGAAAATT
TCATCGCGACAACATTGGACGATTGGCTATTACAAACTGGGTAGCTTGGA
TTTTTGTGAAGAAATGCAAATTCAAATTGAGGATTCTCGCGGAAAAACAA
TCTGTAAAAATACATTTGAAATTTGCATTTGATATTTGGAAAATTGCGTT
TCAATTGAAAACGGAAATGAATGCTAAAATGGCTATTTCGGTTTCTCATT
ACAATCAAGGATTATTACAAAAGGTTATTCACCAATGGCTCTGCGTTAGG
CCGATTGTAAGGCAGTCGAAATTAAACCGAATGAAATCAAATAATCACTT
TGATTCTTTAATTCTAAAACTCGCATTCCAGCAGTGGCGGGAATATCATC
ACAAAACAAAGATAAAACAAAAGGAAAAGGAACGGTGGGATAGTCATTAC
AATTGGAATTTGGCCCACAGATATTTGATGAAATGGAAACAGCATCACGC
ACATCTATTGGTCGAGTATGCTATGGCGGACAGATGCCAGGAAATGCGCA
GGAGATTTCAACTGAGAAAATATTTCATTCAATGGCAGAAATATTATGGA
GAGGAAAAGGTTCTGAGAGTCCAACAGAAATTGGCTGATCAGATGAATGA
GATTCTGATCAAGAGACGAGTCTGGTGTCACTTCAAATCCTTCATTTTGC
AAAGAAGAGAAAAAAATTCCATAAAAGAAAACGCCCAATCATTTGCGAAT
AAATTAATTCTAAGAAGGTATACAGATAAATGGATCTCACGGCTGGAGGA
GAAGGAAAATGTAAATACGAAAACAGAAATGGGAGCAGCAAGATCTCATT
ATCGATTTCCGAAGAGAGATTCTGAACGGGTCATGCACATGAAATATTTC
AGCAAATGGTCACAATTCACGTCCAGCGCGGCTGTTCGCAACCAGAACCT
GTCAAACGCTATCAAAATGTTTGAGACCCGCCTGCTGATGAATGCCATCG
ATTCTTGGCACAGTTTCGTGATCATTTCCAAGGCTAAATCACGATTGATA
AATCTCAAAGAGCATTTCGTAAGAAAGTATGTCCTGTGCAAATATTGGAC
AAAATGGAGACAGGAAAAGAAAGCTCGAGAAAACTTGGAAATTCAAGTCA
ATCAATACGTGGAAATCCAGAAAATCCAGAAGAGAAATAAAATATTTCAC
AAATGGTTTATGTTAACAAAAGTCAAATTAAATGAAAACATTCAAGTTAA
TCGGGTTCAGAATCATGCGAATAGGAAACTTTTATCCAAATGTTTCACAG
CACTTTTACAAAATGTGCAATTGGAGCACCAGAGGAAGATAAATATTGAT
TTGGCATCTATTCATTGGCAACAGAAACTCCAGAACAAAGTATTTATTGT
TTGGCGTATGATTTGTTTGAGGAAAAAACACCAAACCAAATTGTTGTTGT
GGAGGTTTCACAAGACCGAATATCGCATCAAACGGATCGTATTTAATTGT
TGGATAGAATACACCAATCAGATGAAACAGATCCGCGAACAAGTGCAAAT
AATGTTTGAGAGGCAAAGAAACGGAATGGTTATTCAAACATTCCATGCTT
GGAGAACTTATAAACACGAAAGACAATACCGTAAAAGACAGACATCGTAT
AGGCTGGATGAAGGTCAACGGAAATTAGATGTAAAACTCAAACGAAGCAT
ATTTATAAAATGGAAAGCTCAGACACATGTCAGACTATTAGCAATTCAAA
AATCAAAGATAGCTGATGATTTGTATCGGGCAAATTGTCTAAGGAAATAT
CTCGATGCATGGAAGCAATATTATTCAATACAACAGCTGCGTGGTTGGCA
AGCACAAAAGAGCTCTTTGTTTTGGCAGAAATCAGAATTGATTCGAATAT
GGCTAATTTGGAGGCAAGCGCTGGCTGATCGACAACGGGAACATTATTAC
ACTCGTTTTGCATTAATCCACTGGGCAAATAGACTGGAAGCGAAAATATT
TACTCATTGGTTTCGATACACCAATGAAAGAAAAGAAAAGGCCAAAAAAG
AAAATGAAGCATTTTCTCAATACAAAACCCGACTGAAAAAATACACGATG
AGACAATGGGTGACGTCGGGGGACTTTCTCATCCAGTGGAGAAATCAACA
AGCTAATAAACATTTATCGCAATCTGATTGGACCAATTCACAATTTGTGC
AGGATTCAGTAATTCGAGCTATCGAGAAATGGAAACGGTTTACGGCAACA
AGATGCAACAGATCTAATTCGACCAATAGAAAAACCGTAGAAACCTTTCC
ACTGGAACTTAAACAATCCAGCAGGAAATTGTTTCAGAAACAAATTGTTT
CCCAGCAACCGAAAATTCGCAGCCGACCGAAAATTCCCGATTTCATATTA
GAATCCCGACAAATCGACTGGAGATCCTCGACAGCCGGAAACATCGACGC
CCGGAACCAGAAAAATCAATCGGAAAATCGAGTTTACAATTCGGATCTGC
CAGATGAGATATTGTTACCGCCAAAAAGCTTCGAGCGGAATTCCGATTGG
CTGATTACCACAGTTGCGATTCCTGCGACAAATGAGACCAATCAACAGCT
CTATGAGGAACTCAGACTCATCAAAGAAAAGCTGCAGCATTTCAAAGAAT
CGAAATCGATTTTAAAAAATCTCGAGGGCGAACTATTGGAATATGACAGA
GCTATGACGGAATGGAGTCCGAATTGCGATTCGAGTTTCTATGCAGACTT
CAATCCGACAGATTATCGAGCGAGATTGGATCAATCAATTGAACACCTAA
AAATCGATATTTCTCAGCAGAAAAGGATCTGTATCGATTTGTCGAAACGC
TTCCAGGAAATAATGAAGGTAGTCAGCAGGTAATGGTGATCGTTGAAATG
TTTTTAGAGTTGTG
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protein sequence of SMED30017201-orf-1

>SMED30017201-orf-1 ID=SMED30017201-orf-1|Name=SMED30017201-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1148bp
MTQKLEHKKRSILESLNGHLQMSAIRLKNIEIETNILPEKKPLITKIQIK
PSNKMAITRLDPMKTHNVIPLSKNPIIKPNNKPNKSTFKALKPYDPLYKW
NRGGRLKELRIRCQARKFFYIWRNKSIGQVTELLATQHYLRRLKRTTWQI
WYQQYWRLRIEPRLFGRAILFHKSVSIRLVFTTWIKWTRLRLERSRNMEV
ARKFHRDNIGRLAITNWVAWIFVKKCKFKLRILAEKQSVKIHLKFAFDIW
KIAFQLKTEMNAKMAISVSHYNQGLLQKVIHQWLCVRPIVRQSKLNRMKS
NNHFDSLILKLAFQQWREYHHKTKIKQKEKERWDSHYNWNLAHRYLMKWK
QHHAHLLVEYAMADRCQEMRRRFQLRKYFIQWQKYYGEEKVLRVQQKLAD
QMNEILIKRRVWCHFKSFILQRREKNSIKENAQSFANKLILRRYTDKWIS
RLEEKENVNTKTEMGAARSHYRFPKRDSERVMHMKYFSKWSQFTSSAAVR
NQNLSNAIKMFETRLLMNAIDSWHSFVIISKAKSRLINLKEHFVRKYVLC
KYWTKWRQEKKARENLEIQVNQYVEIQKIQKRNKIFHKWFMLTKVKLNEN
IQVNRVQNHANRKLLSKCFTALLQNVQLEHQRKINIDLASIHWQQKLQNK
VFIVWRMICLRKKHQTKLLLWRFHKTEYRIKRIVFNCWIEYTNQMKQIRE
QVQIMFERQRNGMVIQTFHAWRTYKHERQYRKRQTSYRLDEGQRKLDVKL
KRSIFIKWKAQTHVRLLAIQKSKIADDLYRANCLRKYLDAWKQYYSIQQL
RGWQAQKSSLFWQKSELIRIWLIWRQALADRQREHYYTRFALIHWANRLE
AKIFTHWFRYTNERKEKAKKENEAFSQYKTRLKKYTMRQWVTSGDFLIQW
RNQQANKHLSQSDWTNSQFVQDSVIRAIEKWKRFTATRCNRSNSTNRKTV
ETFPLELKQSSRKLFQKQIVSQQPKIRSRPKIPDFILESRQIDWRSSTAG
NIDARNQKNQSENRVYNSDLPDEILLPPKSFERNSDWLITTVAIPATNET
NQQLYEELRLIKEKLQHFKESKSILKNLEGELLEYDRAMTEWSPNCDSSF
YADFNPTDYRARLDQSIEHLKIDISQQKRICIDLSKRFQEIMKVVSR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR030516SFI1
Vocabulary: cellular component
TermDefinition
GO:0005813centrosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1051..1071
NoneNo IPR availablePANTHERPTHR22028UNCHARACTERIZEDcoord: 66..294
coord: 688..1039
coord: 304..507
IPR030516Protein SFI1 homologuePANTHERPTHR22028:SF4PROTEIN SFI1 HOMOLOGcoord: 66..294
coord: 688..1039
coord: 304..507