Acetylcholine receptor subunit beta-like protein

Overview
NameAcetylcholine receptor subunit beta-like protein
Smed IDSMED30017192
Length (bp)1960
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Acetylcholine receptor subunit beta-like protein (SMED30017192) t-SNE clustered cells

Violin plots show distribution of expression levels for Acetylcholine receptor subunit beta-like protein (SMED30017192) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Acetylcholine receptor subunit beta-like protein (SMED30017192) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Acetylcholine receptor subunit beta-like protein (SMED30017192) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30017192

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30017192h1SMcG0014122 dd_Smed_v4_12535_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30017192h1SMcG0014122 dd_Smed_v4_12535_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30017192h1SMcG0014122 dd_Smed_v4_12535_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30017192h1SMcG0014122 dd_Smed_v4_12535_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30017192h1SMcG0014122 dd_Smed_v6_12535_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Human
Match: CHRNB4 (cholinergic receptor nicotinic beta 4 subunit [Source:HGNC Symbol;Acc:HGNC:1964])

HSP 1 Score: 267.314 bits (682), Expect = 2.703e-81
Identity = 168/347 (48.41%), Postives = 236/347 (68.01%), Query Frame = 2
Query:  260 ESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPE-TQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRT 1297
            E KL+  LL   +YN LIRP ++ S  I ++L L++ Q+I+V E+ QIM +NVW++ +WTDY+L W  S +  V++ RI   R+W PDIVL+NNADG YEVS   N++V  NG+VLW+PPAI+ S+C I V+YFPFDQQ+C +KF SWT+D +++ +  +     +D  D+  SG W +V  P R    T   Q P    + Y  I++RK LFYT+NLIIPCVL +LL + VFYLP+D GEKMTL IS+LL+L  FLLLIS+I+PPTS  +PLI K+L+FT ++  F+I  +V ++N + RSP+TH M+ W+++ F+  LP FL M+RP    S +   P  KS  T
Sbjct:   27 EEKLMDDLLNKTRYNNLIRPATSSSQLISIKLQLSLAQLISVNEREQIMTTNVWLKQEWTDYRLTWNSSRYEGVNILRIPAKRIWLPDIVLYNNADGTYEVSVYTNLIVRSNGSVLWLPPAIYKSACKIEVKYFPFDQQNCTLKFRSWTYDHTEIDMVLMTPTASMD--DFTPSGEWDIVALPGRR---TVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLTTLLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMAPWVKRCFLHKLPTFLFMKRPGPDSSPARAFPPSKSCVT 368          

HSP 2 Score: 58.151 bits (139), Expect = 3.570e-8
Identity = 24/70 (34.29%), Postives = 45/70 (64.29%), Query Frame = 2
Query: 1700 LKVSNRMR----SAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            L+ S R R     A+  ++FI++H++ ++ ++ ++ DWKYVA V DR+ L +F  V + GT+G+ L  ++
Sbjct:  414 LRSSGRFRQDVQEALEGVSFIAQHMKNDDEDQSVVEDWKYVAMVVDRLFLWVFMFVCVLGTVGLFLPPLF 483          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Human
Match: CHRNB2 (cholinergic receptor nicotinic beta 2 subunit [Source:HGNC Symbol;Acc:HGNC:1962])

HSP 1 Score: 262.307 bits (669), Expect = 2.357e-79
Identity = 157/332 (47.29%), Postives = 231/332 (69.58%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
            GT  E +L+ +LL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P +F N+   R+    +W PD+VL+NNADG YEVS+  N +V+ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D +++ L  +   +   L D+  SG W +V  P R +     D S    I Y  I++RK LFYT+NLIIPCVLI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M+ W++  F++ LP  L M++P
Sbjct:   25 GTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL--VLKSEVASLDDFTPSGEWDIVALPGRRNE--NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPALLFMQQP 352          

HSP 2 Score: 61.2326 bits (147), Expect = 3.954e-9
Identity = 23/60 (38.33%), Postives = 41/60 (68.33%), Query Frame = 2
Query: 1718 MRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            +R A+  + FI++H+  E++++ +  DWKYVA V DR+ L IF  V + GT+G+ L+ ++
Sbjct:  424 LREAVDGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLF 483          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Human
Match: CHRNB2 (cholinergic receptor nicotinic beta 2 subunit [Source:HGNC Symbol;Acc:HGNC:1962])

HSP 1 Score: 262.307 bits (669), Expect = 3.883e-79
Identity = 157/332 (47.29%), Postives = 231/332 (69.58%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
            GT  E +L+ +LL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P +F N+   R+    +W PD+VL+NNADG YEVS+  N +V+ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D +++ L  +   +   L D+  SG W +V  P R +     D S    I Y  I++RK LFYT+NLIIPCVLI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M+ W++  F++ LP  L M++P
Sbjct:   25 GTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL--VLKSEVASLDDFTPSGEWDIVALPGRRNE--NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPALLFMQQP 352          

HSP 2 Score: 60.8474 bits (146), Expect = 4.319e-9
Identity = 23/60 (38.33%), Postives = 41/60 (68.33%), Query Frame = 2
Query: 1718 MRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            +R A+  + FI++H+  E++++ +  DWKYVA V DR+ L IF  V + GT+G+ L+ ++
Sbjct:  424 LREAVDGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLF 483          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Human
Match: CHRNB2 (cholinergic receptor nicotinic beta 2 subunit [Source:HGNC Symbol;Acc:HGNC:1962])

HSP 1 Score: 257.299 bits (656), Expect = 1.721e-77
Identity = 157/334 (47.01%), Postives = 231/334 (69.16%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRL--KWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
            GT  E +L+ +LL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+    +W DY+L W+P +F N+   R+    +W PD+VL+NNADG YEVS+  N +V+ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D +++ L  +   +   L D+  SG W +V  P R +     D S    I Y  I++RK LFYT+NLIIPCVLI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M+ W++  F++ LP  L M++P
Sbjct:   25 GTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQVSEWEDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL--VLKSEVASLDDFTPSGEWDIVALPGRRNE--NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPALLFMQQP 354          

HSP 2 Score: 61.2326 bits (147), Expect = 4.002e-9
Identity = 23/60 (38.33%), Postives = 41/60 (68.33%), Query Frame = 2
Query: 1718 MRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            +R A+  + FI++H+  E++++ +  DWKYVA V DR+ L IF  V + GT+G+ L+ ++
Sbjct:  426 LREAVDGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLF 485          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Human
Match: CHRNA3 (cholinergic receptor nicotinic alpha 3 subunit [Source:HGNC Symbol;Acc:HGNC:1957])

HSP 1 Score: 255.373 bits (651), Expect = 7.297e-77
Identity = 152/333 (45.65%), Postives = 226/333 (67.87%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
             ++ E +L   L E   YN +IRPV+N+S+ + +   ++M Q++ V+E +QIM++N+W++  W DY+L+W PSD+G  +  R+   ++W+PDIVL+NNA G+++V  +   L+   G V WIPPAIF SSC I V YFPFD Q+C MKFGSW++D +++ L  I     ++L DYW+SG W ++  P    +I     +     I Y++ ++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W++  F+++LP+ + M RP
Sbjct:   31 ASEAEHRLFERLFE--DYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSS--MNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLLPRVMFMTRP 359          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Celegans
Match: acr-3 (Acetylcholine receptor subunit beta-type acr-3 [Source:UniProtKB/Swiss-Prot;Acc:Q93149])

HSP 1 Score: 271.166 bits (692), Expect = 2.136e-83
Identity = 178/352 (50.57%), Postives = 247/352 (70.17%), Query Frame = 2
Query:  194 IFILQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNT-IRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            IF++  LQ     Y N      E +L+ Y+ +   YN LIRPV N S+  I V  +LA + +I V+EK+QIM++NVW  +KW DYQ++W+P +F  +   R+ P +VW PDIVLFNNADGNY VS+  NV+V   G++LW+PPA++ SSC I VE+FPFD+Q C + FGSWTF   +L L Y+ GK+HV+L+DY  SG W ++D P       G      ++I Y I ++RK+LFYTV LI+P VL++ L++ VFYLPA++ EK+TL+ISILL+LVVFLL++S+ILPPTSS+IPL++K+LL TFIMN+ TI ++V+IIN  FR P TH M  W++  F+  LP   +M RPE
Sbjct:   12 IFLITELQC----YPN----SAEERLLSYIFDG--YNSLIRPVLNASSPPIEVFFSLAFVLLINVDEKNQIMQTNVWPTMKWNDYQMQWDPREFDGIKTIRVPPDKVWLPDIVLFNNADGNYLVSFYSNVVVEHTGDMLWVPPAVYKSSCLIDVEFFPFDEQVCSLTFGSWTFRKDELQLSYLSGKRHVELNDYLPSGVWDLIDAP-------GLLIDERSKISYQIKIRRKALFYTVILIMPTVLMAFLSMMVFYLPAESSEKITLAISILLALVVFLLVVSKILPPTSSTIPLMAKYLLMTFIMNMITIMVSVIIINVYFRGPATHIMPNWVKTVFLKFLPVLFVMRRPE 346          

HSP 2 Score: 64.6994 bits (156), Expect = 9.556e-11
Identity = 40/110 (36.36%), Postives = 61/110 (55.45%), Query Frame = 2
Query: 1637 SANDAFFDQRKDFRRNIYPKQL----------KVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKK 1936
            SA   FF +R D + +  PKQ           K+S+  + AI AI +I+ HL  +   K+   +WK+V+ V DR+ L +F  VT  GT+GILL A  + E V++  VI++
Sbjct:  367 SALKTFF-KRNDAKISEQPKQTSRKDGSSSEEKLSSDAKKAIEAIEYITTHLTHDNAFKRQREEWKFVSVVIDRLLLYLFFAVTTGGTVGILLSAPNVFEQVNQTSVIER 475          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Celegans
Match: acr-2 (Acetylcholine receptor subunit beta-type acr-2 [Source:UniProtKB/Swiss-Prot;Acc:P48182])

HSP 1 Score: 262.692 bits (670), Expect = 3.467e-79
Identity = 154/339 (45.43%), Postives = 231/339 (68.14%), Query Frame = 2
Query:  236 QNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNT-IRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
            Q     + E +L+  L   ++YN LIRPV N+S+  + V+  +AMI +I V+EK+QI+++NVW+ +KW D+QL W P+++GN+    +   RVW PDIVLFNNADGNYEVS++ NV V  +G+V W+PPA+F SSC I VE+FPFD+Q C + FGSWT+++ ++ L + +  + V L DY  SG W ++D P ++          E ++V+ ++++RK+LFYTV LIIP VL++ L+V  FYLP D+GEK++     LL  +V  LL+   + P +S+IPL+ K+LL  F++NI  +  TV+I+N  FRS  +HKM  W+RK F++ LP  L+M+RPER
Sbjct:   52 QGDTMGEDEDRLVIDLF--REYNFLIRPVKNVSSPPVVVDFGVAMILLINVDEKNQILQTNVWLTMKWNDFQLAWNPAEYGNISNLHVPSDRVWLPDIVLFNNADGNYEVSFKSNVFVDHHGDVTWVPPAMFKSSCRIDVEWFPFDEQCCTLVFGSWTYNSEEVRLHWYNNIQAVQLHDYSYSGIWDVIDVPGQL---VHKPDLKENKMVFNVVIRRKTLFYTVILIIPTVLMAFLSVMAFYLPVDSGEKVS-LTISLLLALVVFLLLVSKILPPTSNIPLMGKYLLLAFVLNITAVVGTVVIVNIYFRSALSHKMPTWVRKVFLEFLPHLLVMKRPER 384          

HSP 2 Score: 81.2629 bits (199), Expect = 7.295e-16
Identity = 37/86 (43.02%), Postives = 59/86 (68.60%), Query Frame = 2
Query: 1697 QLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSAASL 1954
            Q K+S  M++ + AI +I+EH++ E + KK+  DWKYVA V DR+ L+IF  VT+ GT+GI+  A ++ ++VD+  +I K +A  L
Sbjct:  484 QEKISKEMKTTVEAIAYIAEHMKREMSLKKMRDDWKYVAMVLDRLILLIFFGVTLGGTLGIICSAPHVFDFVDQEAIISKLNAKYL 569          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Celegans
Match: lev-1 (Acetylcholine receptor subunit beta-type lev-1 [Source:UniProtKB/Swiss-Prot;Acc:Q27218])

HSP 1 Score: 260.766 bits (665), Expect = 3.687e-79
Identity = 169/351 (48.15%), Postives = 243/351 (69.23%), Query Frame = 2
Query:  203 LQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNT-IRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERK 1252
            L  L  S+ N+        E +L+  L   + YN L++PV N S   + V++ + ++ +I V+EK Q+M +NVW+ +KW D+QL+W+P D+ N+   R+ P +VW PDIVLFNNADGNYEVS+  NVL+   G VLW+PPAI+ SSC I VE+FPFD Q C + FGSWT++  ++ L ++   + VD S+Y  S  W M+D P+ +        S  ++I + I ++RK+LFYTV LI+P VL++ L V VFYLP  +GEKM L++++LLS+VVFLLL+S+ILPPTSSSIPL++K+LL TF++NI TI +T +I N  FRSP TH++  W+RK F+D+LP  + M+RP RK
Sbjct:   19 LVFLAVSLRNHSTCEDIDAEDRLMVDLF--RGYNSLVQPVRNRSELPMIVKIGMQLVLLINVDEKEQVMHTNVWLTMKWDDFQLKWDPRDYANITQIRVAPEKVWLPDIVLFNNADGNYEVSFMCNVLILSTGTVLWVPPAIYKSSCIIDVEFFPFDDQLCSLTFGSWTYNRDEIKLDFLTSDR-VDFSEYSTSSIWDMMDGPAVL-------TSDRSRIEFQIRIRRKTLFYTVVLILPTVLMAFLNVTVFYLPTASGEKMGLTMNVLLSIVVFLLLVSKILPPTSSSIPLVAKYLLLTFVLNIITIMVTTIICNIYFRSPITHRLPPWVRKVFLDILPLLMCMQRPHRK 359          

HSP 2 Score: 60.4622 bits (145), Expect = 2.604e-9
Identity = 31/78 (39.74%), Postives = 45/78 (57.69%), Query Frame = 2
Query: 1703 KVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKK 1936
            ++S   + AI AI FI+E+   EE  K+   DWK++A+V DR  L  F   T+ GT+GI+  A  + E  DEN  + K
Sbjct:  412 ELSPEAQRAIDAIEFITENRRDEEITKQFRDDWKFIASVVDRFLLYGFFGATVGGTIGIIFTAPSVFETFDENATLVK 489          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Celegans
Match: lev-1 (Acetylcholine receptor subunit beta-type lev-1 [Source:UniProtKB/Swiss-Prot;Acc:Q27218])

HSP 1 Score: 252.292 bits (643), Expect = 3.119e-76
Identity = 156/351 (44.44%), Postives = 225/351 (64.10%), Query Frame = 2
Query:  203 LQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNT-IRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERK 1252
            L  L  S+ N+        E +L+  L   + YN L++PV N S   + V++ + ++ +I V+EK Q+M +NVW+ +KW D+QL+W+P D+ N+   R+ P +VW PDIVLFNNADGNYEVS+  NVL+   G VLW+PPAI+ SSC I VE+FPFD Q C + FGSWT++  ++ L ++   + VD S+Y  S  W M+D P+ +        S  ++I + I ++RK+LFYTV LI+P VL++ L V VFYLP  +GEKM L++++LLS+VVFLLL+S+ILPPTSSSIPL++K                         SP TH++  W+RK F+D+LP  + M+RP RK
Sbjct:   19 LVFLAVSLRNHSTCEDIDAEDRLMVDLF--RGYNSLVQPVRNRSELPMIVKIGMQLVLLINVDEKEQVMHTNVWLTMKWDDFQLKWDPRDYANITQIRVAPEKVWLPDIVLFNNADGNYEVSFMCNVLILSTGTVLWVPPAIYKSSCIIDVEFFPFDDQLCSLTFGSWTYNRDEIKLDFLTSDR-VDFSEYSTSSIWDMMDGPAVL-------TSDRSRIEFQIRIRRKTLFYTVVLILPTVLMAFLNVTVFYLPTASGEKMGLTMNVLLSIVVFLLLVSKILPPTSSSIPLVAK-------------------------SPITHRLPPWVRKVFLDILPLLMCMQRPHRK 334          

HSP 2 Score: 60.4622 bits (145), Expect = 2.340e-9
Identity = 31/78 (39.74%), Postives = 45/78 (57.69%), Query Frame = 2
Query: 1703 KVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKK 1936
            ++S   + AI AI FI+E+   EE  K+   DWK++A+V DR  L  F   T+ GT+GI+  A  + E  DEN  + K
Sbjct:  387 ELSPEAQRAIDAIEFITENRRDEEITKQFRDDWKFIASVVDRFLLYGFFGATVGGTIGIIFTAPSVFETFDENATLVK 464          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Celegans
Match: unc-29 (Acetylcholine receptor subunit beta-type unc-29 [Source:UniProtKB/Swiss-Prot;Acc:P48181])

HSP 1 Score: 251.906 bits (642), Expect = 5.173e-76
Identity = 158/319 (49.53%), Postives = 238/319 (74.61%), Query Frame = 2
Query:  293 KQYNPLIRPVSNLSNT-IRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            + YN LI+PV N S   + V++AL ++ +I V+EK Q+M +NVW+ L+W D+Q++W P ++G +   R++P +VW PDIVLFNNADGNYEVS+  NV++   G++LW+PPAI+ SSC I VE+FPFD+Q C + FGSWT++ +++ L ++  +  VD+S+Y  S  W ++D P+ + N         ++I + + ++RK+LFYTV LIIP VL++ L++ VF+LP D+GEK+TL+IS+LLS+VVFLLL+S+ILPPTSS+IPL++K+LL TF++N+ TI +TV+IIN  FR P TH+M  W+R  F+  LPK + M+RP+
Sbjct:   39 RGYNSLIQPVRNSSELPLIVKMALQLVLLINVDEKDQVMHTNVWLTLQWHDFQMKWNPVNYGEIKQIRVSPDKVWLPDIVLFNNADGNYEVSFMCNVVINHKGDMLWVPPAIYKSSCIIDVEFFPFDEQVCTLVFGSWTYNENEIKLEFVQAEL-VDVSEYSASSIWDVIDVPASLVN-------KRSRIEFQVRIRRKTLFYTVVLIIPTVLMAFLSMAVFFLPTDSGEKITLTISVLLSIVVFLLLVSKILPPTSSTIPLMAKYLLLTFVLNVITILVTVIIINVYFRGPRTHRMPQWVRVVFLQFLPKLVCMKRPK 349          

HSP 2 Score: 70.4774 bits (171), Expect = 1.538e-12
Identity = 33/79 (41.77%), Postives = 51/79 (64.56%), Query Frame = 2
Query: 1727 AISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVI----KKYSAAS 1951
            AI AI +I+EHL+ +E +K    DWKYVA + DR+ L +F  +T+ GT GIL  A ++ + +D+ E++    +KY  AS
Sbjct:  412 AIDAIEYITEHLKRDEQHKSFRDDWKYVAMIIDRLLLYVFFGITVGGTCGILFSAPHVFQRIDQQEMLDRLKEKYDTAS 490          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Fly
Match: nAChRbeta1 (gene:FBgn0000038 transcript:FBtr0073299)

HSP 1 Score: 329.717 bits (844), Expect = 3.317e-105
Identity = 198/335 (59.10%), Postives = 255/335 (76.12%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSP-ETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
             ++ E +L+  L   + YN LIRPV N++  + V   LA +Q+I V EK+QIMKSNVW+RL W DYQL+W+ +D+G + V R+ P +VW+PDIVLFNNADGNYEV ++ NVL+ P G VLW+PPAI+ SSCTI V YFPFDQQ+C MKFGSWTF+  Q++L   + K  VDLSDYWKSG+W +++ P+ ++   G    P ET I + II++RK+LFYTVNLI+P VLIS L V VFYLPA+AGEK+TL ISILLSLVVFLLL+S+ILPPTS  +PLI+K+LLFTFIMN  +I +TV+IINWNFR P TH+M  +IR  F+  LP FL M+RP +
Sbjct:   24 ASEDEERLVRDLF--RGYNKLIRPVQNMTQKVGVRFGLAFVQLINVNEKNQIMKSNVWLRLVWYDYQLQWDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKSNVLIYPTGEVLWVPPAIYQSSCTIDVTYFPFDQQTCIMKFGSWTFNGDQVSLALYNNKNFVDLSDYWKSGTWDIIEVPAYLNVYEGDSNHPTETDITFYIIIRRKTLFYTVNLILPTVLISFLCVLVFYLPAEAGEKVTLGISILLSLVVFLLLVSKILPPTSLVLPLIAKYLLFTFIMNTVSILVTVIIINWNFRGPRTHRMPMYIRSIFLHYLPAFLFMKRPRK 356          

HSP 2 Score: 79.337 bits (194), Expect = 3.661e-15
Identity = 39/80 (48.75%), Postives = 52/80 (65.00%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSA 1945
            +S     A  A+ FI+EHL  E+   +   DWKYVA V DR+QL IF IVT AGT+GIL+ A +I EYVD++ +I+ Y  
Sbjct:  441 LSPEASKATEAVEFIAEHLRNEDLYIQTREDWKYVAMVIDRLQLYIFFIVTTAGTVGILMDAPHIFEYVDQDRIIEIYRG 520          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Fly
Match: nAChRalpha4 (gene:FBgn0266347 transcript:FBtr0332872)

HSP 1 Score: 256.914 bits (655), Expect = 1.173e-77
Identity = 154/330 (46.67%), Postives = 226/330 (68.48%), Query Frame = 2
Query:  299 YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKK-------HVDLSDYWKSGSWYMVDCPS-RMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMS 1264
            YN L+RPV N ++ ++V + L + Q+I V  K+QIM +N+W+   W DY+LRWEP ++G V +  +    +W+PDIVL+NNADGN+EV+      +   G V W PPAI+ SSC I VEYFPFD+Q+C +KFGSWT+D  ++ L ++D ++        VDLS+++ S  W +++ P+ R +        P   I + I ++RK+LFYTVN+IIPC+ IS LTV  FYLP+D+GEK+TLSISIL+SL VF LL+  I+PPTS  +PL+ K+L+F  I+   +I +TV+++N +FRSP THKM+ W+++ FID LP FL ++RP   +  S
Sbjct:   23 YNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLPAFLFIKRPTYNFETS 352          

HSP 2 Score: 64.6994 bits (156), Expect = 1.370e-10
Identity = 35/101 (34.65%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1592 THCDTFECPSSPS-DISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKA 1891
            THC + E  + P  DI         +     N      K   ++  +   + FI+EH +M+E++ K+  DWKYVA V DR+ L IFT+  + GT GI+L+A
Sbjct:  373 THCSSDEIAAVPDMDIGP----LGMKSPILNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQA 469          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Fly
Match: nAChRalpha4 (gene:FBgn0266347 transcript:FBtr0300118)

HSP 1 Score: 257.299 bits (656), Expect = 2.264e-77
Identity = 154/330 (46.67%), Postives = 226/330 (68.48%), Query Frame = 2
Query:  299 YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKK-------HVDLSDYWKSGSWYMVDCPS-RMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMS 1264
            YN L+RPV N ++ ++V + L + Q+I V  K+QIM +N+W+   W DY+LRWEP ++G V +  +    +W+PDIVL+NNADGN+EV+      +   G V W PPAI+ SSC I VEYFPFD+Q+C +KFGSWT+D  ++ L ++D ++        VDLS+++ S  W +++ P+ R +        P   I + I ++RK+LFYTVN+IIPC+ IS LTV  FYLP+D+GEK+TLSISIL+SL VF LL+  I+PPTS  +PL+ K+L+F  I+   +I +TV+++N +FRSP THKM+ W+++ FID LP FL ++RP   +  S
Sbjct:   23 YNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEIDVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLPAFLFIKRPTYNFETS 352          

HSP 2 Score: 64.6994 bits (156), Expect = 1.413e-10
Identity = 35/101 (34.65%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1592 THCDTFECPSSPS-DISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKA 1891
            THC + E  + P  DI         +     N      K   ++  +   + FI+EH +M+E++ K+  DWKYVA V DR+ L IFT+  + GT GI+L+A
Sbjct:  413 THCSSDEIAAVPDMDIGP----LGMKSPILNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQA 509          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Fly
Match: nAChRalpha4 (gene:FBgn0266347 transcript:FBtr0346707)

HSP 1 Score: 256.914 bits (655), Expect = 3.298e-77
Identity = 153/330 (46.36%), Postives = 228/330 (69.09%), Query Frame = 2
Query:  299 YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKK-------HVDLSDYWKSGSWYMVDCPS-RMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMS 1264
            YN L+RPV N ++ ++V + L + Q+I V  K+QIM +N+W+   W DY+LRWEP ++G V +  +    +W+PDIVL+NNADG+YEV+     +V  NG V+W PPA++ SSC+I VEYFP+D Q+C +K GSWT+D  ++ L ++D ++        VDLS+++ S  W +++ P+ R +        P   I + I ++RK+LFYTVN+IIPC+ IS LTV  FYLP+D+GEK+TLSISIL+SL VF LL+  I+PPTS  +PL+ K+L+F  I+   +I +TV+++N +FRSP THKM+ W+++ FID LP FL ++RP   +  S
Sbjct:   23 YNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGHYEVTLMTKAIVYNNGLVIWQPPAVYKSSCSIDVEYFPYDVQTCILKLGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFIDFLPAFLFIKRPTYNFETS 352          

HSP 2 Score: 65.0846 bits (157), Expect = 1.240e-10
Identity = 35/101 (34.65%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1592 THCDTFECPSSPS-DISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKA 1891
            THC + E  + P  DI         +     N      K   ++  +   + FI+EH +M+E++ K+  DWKYVA V DR+ L IFT+  + GT GI+L+A
Sbjct:  413 THCSSDEIAAVPDMDIGP----LGMKSPILNNPAFSHSKCLPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYVAMVLDRLFLWIFTLAVVVGTAGIILQA 509          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Fly
Match: nAChRalpha1 (gene:FBgn0000036 transcript:FBtr0334916)

HSP 1 Score: 254.218 bits (648), Expect = 6.171e-76
Identity = 154/318 (48.43%), Postives = 220/318 (69.18%), Query Frame = 2
Query:  299 YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYI-------DGKKHVDLSDYWKSGSWYMVDCPS-RMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFL 1228
            YN LIRPV N S+ + V++ L + Q+I V  K+QIM +NVW+  +W DY+L+W P D+G VD   +    +W PDIVL+NNADGNYEV+     ++   G V+W PPAI+ S C I VEYFPFD+Q+C MKFGSWT+D   + L ++       + +  +DL DY+ S  W ++  P+ R +      + P   IV+ + L+RK+LFYTVNLIIPCV IS L+V VFYLP+D+GEK++L ISILLSL VF LL++ I+PPTS ++PL+ K+LLFT ++   ++ +T+ ++N NFRSP TH+M+ W+++ FI +LPK L
Sbjct:   36 YNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWVEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNADGNYEVTIMTKAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYMVDLRHLKQTADSDNIEVGIDLQDYYISVEWDIMRVPAVRNEKFYSCCEEPYLDIVFNLTLRRKTLFYTVNLIIPCVGISFLSVLVFYLPSDSGEKISLCISILLSLTVFFLLLAEIIPPTSLTVPLLGKYLLFTMMLVTLSVVVTIAVLNVNFRSPVTHRMAPWVQRLFIQILPKLL 353          

HSP 2 Score: 50.447 bits (119), Expect = 3.905e-6
Identity = 23/61 (37.70%), Postives = 36/61 (59.02%), Query Frame = 2
Query: 1709 SNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKA 1891
            +  M   I    FI++H++ ++  + +  DWKYVA V DR+ L IF I  + GT  I+L+A
Sbjct:  474 AREMEKTIEGSRFIAQHVKNKDKFESVEEDWKYVAMVLDRMFLWIFAIACVVGTALIILQA 534          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Zebrafish
Match: chrna3 (cholinergic receptor, nicotinic, alpha 3 [Source:ZFIN;Acc:ZDB-GENE-070822-1])

HSP 1 Score: 268.085 bits (684), Expect = 2.414e-81
Identity = 158/329 (48.02%), Postives = 231/329 (70.21%), Query Frame = 2
Query:  299 YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNV 1282
            YN  IRPV N+S+ + V+  ++M Q++ V+E +QIM++N+W+R  W DY+LRW+P DFG V+  R+  +++W+PDIVL+NNA G+++V  +   L+  NG+V WIPPAIF SSC I V YFPFD Q+C MKFGSWT+D +++ L  I     ++L D+W+SG W ++D P    +I     +   T I Y++ ++R  LFYT+N+IIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W+R+ F+ +LP+ + M RPE+   +  K+ +V
Sbjct:   75 YNQYIRPVENVSDPVVVQFEVSMSQLVKVDEVNQIMETNLWLRHIWNDYKLRWDPKDFGGVEFIRVPSNKIWKPDIVLYNNAVGDFQVDDKTKALLRFNGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLIGST--INLKDFWESGEWTIIDAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPCWVRRVFLSLLPRVMFMTRPEKDQEVPAKQCSV 401          

HSP 2 Score: 61.6178 bits (148), Expect = 1.788e-9
Identity = 27/67 (40.30%), Postives = 45/67 (67.16%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILE 1906
            VS ++R AI ++ +I+E++ ++   K++  DWKYVA V DRI L +F +V I GT G+ L+ + + E
Sbjct:  475 VSPQVREAIESVKYIAENMRLQNEAKEVQDDWKYVAMVIDRIFLWVFVLVCILGTAGLFLQPLLLGE 541          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Zebrafish
Match: chrna2a (cholinergic receptor, nicotinic, alpha 2a (neuronal) [Source:ZFIN;Acc:ZDB-GENE-040108-2])

HSP 1 Score: 257.299 bits (656), Expect = 1.701e-77
Identity = 170/354 (48.02%), Postives = 234/354 (66.10%), Query Frame = 2
Query:  191 VIFILQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPET--QIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            +I I  H+Q  +++    +    E KL H L     YN   RPV N+S+ + V+  L++ Q+I V+EK+Q+M +NVW++ +W DY+LRW PS+F NV   R+    +W PDIVL+NNADG + V+      +   G V W+PPAI+ SSC+I V +FPFDQQ+C+MKFGSWT+D +++ L  I+G   VDL DYW+SG W +++      N    D   E    I Y  I++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLLLI+ I+P TS  IPLI ++LLFT I    +I ITV ++N + RSP+TH M  W+   F+D +P++L M RPE
Sbjct:   16 LILIADHVQPVLSD--GWIHAHAEDKLFHKLF--IGYNKWSRPVRNISDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPSEFDNVTSIRVPSEMIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVSWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQIEGT--VDLKDYWESGEWAIINAVGTY-NTKKYDCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHSMPRWVHSVFLDHIPRWLFMRRPE 362          

HSP 2 Score: 54.6842 bits (130), Expect = 2.481e-7
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 2
Query: 1727 AISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILL 1885
            A+  + +I++HL  E+ +  +  DWKYVA V DRI L +F IV + GT+G+ L
Sbjct:  460 ALEGVRYIADHLRAEDEDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTIGLFL 512          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Zebrafish
Match: chrna4a (cholinergic receptor, nicotinic, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-130530-903])

HSP 1 Score: 254.988 bits (650), Expect = 2.472e-76
Identity = 209/554 (37.73%), Postives = 311/554 (56.14%), Query Frame = 2
Query:  251 TQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPS--DFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPE--TQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLSQXXXXXXXXXXXXXXLFANPWNNYQN-----MSRHSLDSESDKIPDSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILL 1885
             Q E +L++ LL    Y+ L RPV ++S+ + V   L++ Q+I V+EK+Q+M +NVW++ +W DY+LRW P   D GNV   RI    +W+PDIVL   ADG++ V+      +  +G V WIPPAI+ SSC+I V + PFDQQ+C +KFGSWT+D S++ L  I     VD  DYW+SG W +    +   N+   +   E    I Y+ I++R  LFYT+NLIIPC+LI  LTV VFYLP+D GE++T+ IS+LLSL VFLLLI+ I+PPTS  IPLI+++LLF  I  I  I ITV ++N + RSP+TH M  W+R++F+DV+P+F+ ++RP+        + + K V      +N   VD    N    +   S   K Y S    +++S            +P   Y N     M  H   S+S ++   +R    C++ST    RS     +C   E     S +   D F     +  + +       S+  R AI  + +I+E+L  +  +  +  +WKYVA V DR+ L +F +V I G +G+ +
Sbjct:   22 VQAEERLLNSLL--SAYSKLTRPVHSVSDVVHVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWLDYRLRWTPGPEDNGNVSSMRIPAQFIWKPDIVL---ADGDFTVTHLTKAHLLHDGRVTWIPPAIYKSSCSIDVTFLPFDQQNCTIKFGSWTYDRSKIDL--IRIADSVDQLDYWESGEWLITSAVATY-NVKKYECCSEEYADITYSFIIRRLPLFYTINLIIPCLLILCLTVLVFYLPSDCGEEVTMCISVLLSLTVFLLLITEIIPPTSLVIPLIAEYLLFIMIFIILFIIITVFVLNVHHRSPDTHSMPQWVRRFFLDVMPRFIFIQRPQI------WQKDAKGVCDDAQKSNKRCVDSNWKNRE--IKEESIRMKVYSSCLNADMHS----------IKSPSQAYNNSTCPKMKLHKGRSQSVQVNCGERDVNLCNSSTRFRSRS---MQYCSLHEHEQQTSAVFNADKF----SNICKVMTSTGENPSHATRLAIEGVQYIAEYLRAQHADLSVKEEWKYVAMVIDRLFLWMFVLVCILGIVGLFI 542          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Zebrafish
Match: chrnb5a (cholinergic receptor, nicotinic, beta 5a [Source:ZFIN;Acc:ZDB-GENE-090312-169])

HSP 1 Score: 251.521 bits (641), Expect = 1.293e-75
Identity = 161/355 (45.35%), Postives = 238/355 (67.04%), Query Frame = 2
Query:  191 VIFILQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPET--QIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
            +I +   L +SV  +++ +    E +L+ +LL  ++YN LIRP  N S  + + + +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P+++  +   RI    +W PDIVL+NNADG YEVS+  N +V+  G++ W+PPAI+ S+C I V  FPFDQQ+C +KF SWT+D ++L L         D  DY  SG W +V  P R +     D S  T   + Y  +++RK LFYT+NLIIPCVLI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS ++PLI K+L+FT ++  F+I  +V ++N + RSP+TH+M  W+++ F+  LP FL+M RP R
Sbjct:    5 LIRLTATLTDSVCLHRS-LAADAEERLVDFLLGPERYNKLIRPAVNKSQQVTIGIKVSLAQLISVNEREQIMTTNVWLTQEWNDYRLVWDPNEYEGIKKLRIPSRHIWLPDIVLYNNADGVYEVSFYCNAVVSNTGDIFWLPPAIYKSACAIEVRNFPFDQQNCTLKFRSWTYDRTELDLYLTSDFASRD--DYTPSGEWDIVSLPGRRNE----DLSDPTYLDVTYDFVIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLAVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHRMPEWVKRVFLHKLPFFLLMRRPGR 352          

HSP 2 Score: 63.929 bits (154), Expect = 3.665e-10
Identity = 24/58 (41.38%), Postives = 43/58 (74.14%), Query Frame = 2
Query: 1724 SAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
             AI  + FI+EH+++E++++ I+ DWKYVA V DR+ L IF +V + GT+G+ ++ ++
Sbjct:  420 EAIEGVRFIAEHMKIEDDDEGIIEDWKYVAMVIDRLFLWIFILVCVVGTLGLFVQPLF 477          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Zebrafish
Match: chrnb5a (cholinergic receptor, nicotinic, beta 5a [Source:ZFIN;Acc:ZDB-GENE-090312-169])

HSP 1 Score: 250.366 bits (638), Expect = 2.038e-75
Identity = 157/337 (46.59%), Postives = 228/337 (67.66%), Query Frame = 2
Query:  245 VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPET--QIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
            +    E +L+ +LL  ++YN LIRP  N S  + + + +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P+++  +   RI    +W PDIVL+NNADG YEVS+  N +V+  G++ W+PPAI+ S+C I V  FPFDQQ+C +KF SWT+D ++L L         D  DY  SG W +V  P R +     D S  T   + Y  +++RK LFYT+NLIIPCVLI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS ++PLI K+L+FT ++  F+I  +V ++N + RSP+TH+M  W+++ F+  LP FL+M RP R
Sbjct:    3 LAADAEERLVDFLLGPERYNKLIRPAVNKSQQVTIGIKVSLAQLISVNEREQIMTTNVWLTQEWNDYRLVWDPNEYEGIKKLRIPSRHIWLPDIVLYNNADGVYEVSFYCNAVVSNTGDIFWLPPAIYKSACAIEVRNFPFDQQNCTLKFRSWTYDRTELDLYLTSDFASRD--DYTPSGEWDIVSLPGRRNE----DLSDPTYLDVTYDFVIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLAVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHRMPEWVKRVFLHKLPFFLLMRRPGR 333          

HSP 2 Score: 63.929 bits (154), Expect = 3.388e-10
Identity = 24/58 (41.38%), Postives = 43/58 (74.14%), Query Frame = 2
Query: 1724 SAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
             AI  + FI+EH+++E++++ I+ DWKYVA V DR+ L IF +V + GT+G+ ++ ++
Sbjct:  401 EAIEGVRFIAEHMKIEDDDEGIIEDWKYVAMVIDRLFLWIFILVCVVGTLGLFVQPLF 458          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Xenopus
Match: chrnb2 (cholinergic receptor nicotinic beta 2 subunit [Source:NCBI gene;Acc:100101688])

HSP 1 Score: 261.536 bits (667), Expect = 3.270e-79
Identity = 157/345 (45.51%), Postives = 236/345 (68.41%), Query Frame = 2
Query:  215 QNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
            QN   +  + +GT  E +L+ +LL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P++F ++   R+    +W PD+VL+NNADG YEVS+  N +V+ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D ++L L  +       L D+  SG W ++  P R +     + S    I Y  I++RK LFYT+NLIIPC+LI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M  W++  F+D LP  L M++P +
Sbjct:   14 QNPQRDKYSCLGTDTEERLVEHLLDPSRYNKLIRPATNGSEQVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWDPAEFDDMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSHDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTELDL--VLRSDVASLDDFTPSGEWDIIALPGRRNE--NPNDSTYVDITYDFIIRRKPLFYTINLIIPCILITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMPPWVKVVFLDKLPTLLFMKQPRQ 354          

HSP 2 Score: 60.077 bits (144), Expect = 8.143e-9
Identity = 33/125 (26.40%), Postives = 64/125 (51.20%), Query Frame = 2
Query: 1523 DSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            D  R+ CTC  +  ++ + G   +     E P         + F D++   R+ +          ++ A+  + FI++H++ E++++ +  DWKYVA V DR+ L IF  V + GT+G+ L+ ++
Sbjct:  379 DGARS-CTCYVNQASVKKYGAQLS-----ELPE------GVNGFRDRQGKVRQCLC--------GLQEAVDGVRFIADHMKSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLF 483          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Xenopus
Match: chrnb2 (cholinergic receptor nicotinic beta 2 subunit [Source:NCBI gene;Acc:100101688])

HSP 1 Score: 261.151 bits (666), Expect = 3.970e-79
Identity = 155/335 (46.27%), Postives = 232/335 (69.25%), Query Frame = 2
Query:  245 VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
            +GT  E +L+ +LL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P++F ++   R+    +W PD+VL+NNADG YEVS+  N +V+ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D ++L L  +       L D+  SG W ++  P R +     + S    I Y  I++RK LFYT+NLIIPC+LI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M  W++  F+D LP  L M++P +
Sbjct:   21 LGTDTEERLVEHLLDPSRYNKLIRPATNGSEQVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWDPAEFDDMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSHDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTELDL--VLRSDVASLDDFTPSGEWDIIALPGRRNE--NPNDSTYVDITYDFIIRRKPLFYTINLIIPCILITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMPPWVKVVFLDKLPTLLFMKQPRQ 351          

HSP 2 Score: 59.6918 bits (143), Expect = 1.033e-8
Identity = 33/125 (26.40%), Postives = 64/125 (51.20%), Query Frame = 2
Query: 1523 DSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            D  R+ CTC  +  ++ + G   +     E P         + F D++   R+ +          ++ A+  + FI++H++ E++++ +  DWKYVA V DR+ L IF  V + GT+G+ L+ ++
Sbjct:  376 DGARS-CTCYVNQASVKKYGAQLS-----ELPE------GVNGFRDRQGKVRQCL--------CGLQEAVDGVRFIADHMKSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLF 480          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Xenopus
Match: usp16 (ubiquitin specific peptidase 16 [Source:Xenbase;Acc:XB-GENE-1007164])

HSP 1 Score: 258.455 bits (659), Expect = 3.152e-78
Identity = 159/333 (47.75%), Postives = 229/333 (68.77%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
             T  E +L++ LL   +YN LIRP  N S  + ++L L++ Q+I+V E+ QIM +NVW+  +W DY+L W P+D+  +   RI   ++W PDIVL+NNADG YEVS   N +V  NG+V W+PPAI+ S+C I V++FPFDQQ+C +KF SWT+D +++ L  +  K    + D+  SG W +++ P R   I   D +    + Y  I++RK LFYT+NLIIPC+LI+ L + VFYLP+D G+KMTL IS+LL+L VFLLLIS+I+PPTS  +PLI K+L+FT ++  F+I  +V ++N + RSP+TH M  W++  F+D LP FL M+RPE
Sbjct:   24 STDSEERLMNNLLRKDKYNKLIRPAYNNSQLVSIQLQLSLAQLISVNEREQIMTTNVWLNQEWVDYRLTWNPADYDGIKKLRIPSQQIWLPDIVLYNNADGTYEVSLFTNAIVQNNGSVAWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDYTEIDL--VLNKGWATMDDFTPSGEWDIIELPGRR-TICPLDPT-YVDVTYDFIIKRKPLFYTINLIIPCILITSLAILVFYLPSDCGQKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMPPWVKNCFLDRLPVFLFMKRPE 352          

HSP 2 Score: 68.9366 bits (167), Expect = 1.046e-11
Identity = 35/92 (38.04%), Postives = 54/92 (58.70%), Query Frame = 2
Query: 1619 SSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAV 1894
            + P D + ND     R    R  YP+  K +  +  A+  + FISEH++ E+ ++ I+ DWKYVA V DR+ L IF IV I GT+G+ ++ +
Sbjct:  384 ARPCDHTVNDPPESVRHQEFRYRYPR--KYTPEVHEAMDGVKFISEHMKSEDEDQSIIEDWKYVAMVVDRLFLWIFIIVCIVGTVGLFMQPL 473          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Xenopus
Match: usp16 (ubiquitin specific peptidase 16 [Source:Xenbase;Acc:XB-GENE-1007164])

HSP 1 Score: 256.914 bits (655), Expect = 9.510e-78
Identity = 158/329 (48.02%), Postives = 228/329 (69.30%), Query Frame = 2
Query:  260 ESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            E +L++ LL   +YN LIRP  N S  + ++L L++ Q+I+V E+ QIM +NVW+  +W DY+L W P+D+  +   RI   ++W PDIVL+NNADG YEVS   N +V  NG+V W+PPAI+ S+C I V++FPFDQQ+C +KF SWT+D +++ L  +  K    + D+  SG W +++ P R   I   D +    + Y  I++RK LFYT+NLIIPC+LI+ L + VFYLP+D G+KMTL IS+LL+L VFLLLIS+I+PPTS  +PLI K+L+FT ++  F+I  +V ++N + RSP+TH M  W++  F+D LP FL M+RPE
Sbjct:   10 EERLMNNLLRKDKYNKLIRPAYNNSQLVSIQLQLSLAQLISVNEREQIMTTNVWLNQEWVDYRLTWNPADYDGIKKLRIPSQQIWLPDIVLYNNADGTYEVSLFTNAIVQNNGSVAWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDYTEIDL--VLNKGWATMDDFTPSGEWDIIELPGRR-TICPLDPT-YVDVTYDFIIKRKPLFYTINLIIPCILITSLAILVFYLPSDCGQKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMPPWVKNCFLDRLPVFLFMKRPE 334          

HSP 2 Score: 69.3218 bits (168), Expect = 9.428e-12
Identity = 35/92 (38.04%), Postives = 54/92 (58.70%), Query Frame = 2
Query: 1619 SSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAV 1894
            + P D + ND     R    R  YP+  K +  +  A+  + FISEH++ E+ ++ I+ DWKYVA V DR+ L IF IV I GT+G+ ++ +
Sbjct:  366 ARPCDHTVNDPPESVRHQEFRYRYPR--KYTPEVHEAMDGVKFISEHMKSEDEDQSIIEDWKYVAMVVDRLFLWIFIIVCIVGTVGLFMQPL 455          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Xenopus
Match: usp16 (ubiquitin specific peptidase 16 [Source:Xenbase;Acc:XB-GENE-1007164])

HSP 1 Score: 256.914 bits (655), Expect = 2.198e-77
Identity = 158/333 (47.45%), Postives = 228/333 (68.47%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
                E +L++ LL   +YN LIRP  N S  + ++L L++ Q+I+V E+ QIM +NVW+  +W DY+L W P+D+  +   RI   ++W PDIVL+NNADG YEVS   N +V  NG+V W+PPAI+ S+C I V++FPFDQQ+C +KF SWT+D +++ L  +  K    + D+  SG W +++ P R   I   D +    + Y  I++RK LFYT+NLIIPC+LI+ L + VFYLP+D G+KMTL IS+LL+L VFLLLIS+I+PPTS  +PLI K+L+FT ++  F+I  +V ++N + RSP+TH M  W++  F+D LP FL M+RPE
Sbjct:   44 AADSEERLMNNLLRKDKYNKLIRPAYNNSQLVSIQLQLSLAQLISVNEREQIMTTNVWLNQEWVDYRLTWNPADYDGIKKLRIPSQQIWLPDIVLYNNADGTYEVSLFTNAIVQNNGSVAWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDYTEIDL--VLNKGWATMDDFTPSGEWDIIELPGR-RTICPLDPT-YVDVTYDFIIKRKPLFYTINLIIPCILITSLAILVFYLPSDCGQKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMPPWVKNCFLDRLPVFLFMKRPE 372          

HSP 2 Score: 70.8626 bits (172), Expect = 3.051e-12
Identity = 36/92 (39.13%), Postives = 55/92 (59.78%), Query Frame = 2
Query: 1619 SSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAV 1894
            + P D + ND     R    R  YP+  K +  +  A+  + FISEH++ E+ ++ I+ DWKYVA V DR+ L IF IV I GT+G+ ++A+
Sbjct:  404 ARPCDHTVNDPPESVRHQEFRYRYPR--KYTPEVHEAMDGVKFISEHMKSEDEDQSIIEDWKYVAMVVDRLFLWIFIIVCIVGTVGLFMQAM 493          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Mouse
Match: Chrnb2 (cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal) [Source:MGI Symbol;Acc:MGI:87891])

HSP 1 Score: 263.077 bits (671), Expect = 8.847e-80
Identity = 156/336 (46.43%), Postives = 232/336 (69.05%), Query Frame = 2
Query:  245 VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERK 1252
            +GT  E +L+ +LL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P DF N+   R+    +W PD+VL+NNADG YEVS+  N +V+ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D +++ L  +       L D+  SG W ++  P R +     D S    I Y  I++RK LFYT+NLIIPCVLI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M+ W++  F++ LP  L +++P  +
Sbjct:   24 LGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL--VLKSDVASLDDFTPSGEWDIIALPGRRNE--NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPTLLFLQQPRHR 355          

HSP 2 Score: 65.0846 bits (157), Expect = 1.642e-10
Identity = 32/108 (29.63%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 1574 RSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            R G     C  F  P+S   ++             R++ P     S  +R A+  + FI++H+  E++++ +  DWKYVA V DR+ L IF  V + GT+G+ L+ ++
Sbjct:  379 REGPAADPCTCFVNPASMQGLAGAFQAEPAAAGLGRSMGP----CSCGLREAVDGVRFIADHMRSEDDDQSVREDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLF 482          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Mouse
Match: Chrna3 (cholinergic receptor, nicotinic, alpha polypeptide 3 [Source:MGI Symbol;Acc:MGI:87887])

HSP 1 Score: 261.151 bits (666), Expect = 1.825e-79
Identity = 154/333 (46.25%), Postives = 229/333 (68.77%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
             ++ E +L  YL E   YN +IRPV+N+S+ + ++  ++M Q++ V+E +QIM++N+W++  W DY+L+W+PSD+  V+  R+   ++W+PDIVL+NNADG+++V  +   L+   G V WIPPAIF SSC I V YFPFD Q+C MKFGSW++D +++ L  I     ++L DYW+SG W ++  P     I     +     I Y++ ++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W++  F+++LP+ + M RP
Sbjct:   25 ASEAEHRLFQYLFE--DYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSS--MNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRP 353          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Mouse
Match: Chrna3 (cholinergic receptor, nicotinic, alpha polypeptide 3 [Source:MGI Symbol;Acc:MGI:87887])

HSP 1 Score: 261.151 bits (666), Expect = 4.412e-79
Identity = 154/333 (46.25%), Postives = 229/333 (68.77%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
             ++ E +L  YL E   YN +IRPV+N+S+ + ++  ++M Q++ V+E +QIM++N+W++  W DY+L+W+PSD+  V+  R+   ++W+PDIVL+NNADG+++V  +   L+   G V WIPPAIF SSC I V YFPFD Q+C MKFGSW++D +++ L  I     ++L DYW+SG W ++  P     I     +     I Y++ ++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W++  F+++LP+ + M RP
Sbjct:   25 ASEAEHRLFQYLFE--DYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSS--MNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRP 353          

HSP 2 Score: 56.225 bits (134), Expect = 9.346e-8
Identity = 25/63 (39.68%), Postives = 42/63 (66.67%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAV 1894
            +S  ++ AI ++ +I+E+++ +   K+I  DWKYVA V DRI L +F +V I GT G+ L+ +
Sbjct:  431 LSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPL 493          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Mouse
Match: Chrna3 (cholinergic receptor, nicotinic, alpha polypeptide 3 [Source:MGI Symbol;Acc:MGI:87887])

HSP 1 Score: 261.151 bits (666), Expect = 4.771e-79
Identity = 154/333 (46.25%), Postives = 229/333 (68.77%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
             ++ E +L  YL E   YN +IRPV+N+S+ + ++  ++M Q++ V+E +QIM++N+W++  W DY+L+W+PSD+  V+  R+   ++W+PDIVL+NNADG+++V  +   L+   G V WIPPAIF SSC I V YFPFD Q+C MKFGSW++D +++ L  I     ++L DYW+SG W ++  P     I     +     I Y++ ++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W++  F+++LP+ + M RP
Sbjct:   30 ASEAEHRLFQYLFE--DYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSS--MNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRP 358          

HSP 2 Score: 56.225 bits (134), Expect = 9.834e-8
Identity = 25/63 (39.68%), Postives = 42/63 (66.67%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAV 1894
            +S  ++ AI ++ +I+E+++ +   K+I  DWKYVA V DRI L +F +V I GT G+ L+ +
Sbjct:  436 LSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPL 498          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Mouse
Match: Chrnb2 (cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal) [Source:MGI Symbol;Acc:MGI:87891])

HSP 1 Score: 260.766 bits (665), Expect = 8.683e-77
Identity = 156/338 (46.15%), Postives = 233/338 (68.93%), Query Frame = 2
Query:  245 VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYS 1258
            +GT  E +L+ +LL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P DF N+   R+    +W PD+VL+NNADG YEVS+  N +V+ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D +++ L  +       L D+  SG W ++  P R +     D S    I Y  I++RK LFYT+NLIIPCVLI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M+ W++  F++ LP  L +++P  + +
Sbjct:   24 LGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL--VLKSDVASLDDFTPSGEWDIIALPGRRNE--NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPTLLFLQQPRHRCA 357          
BLAST of Acetylcholine receptor subunit beta-like protein vs. UniProt/SwissProt
Match: sp|P04755|ACH3_DROME (Acetylcholine receptor subunit beta-like 1 OS=Drosophila melanogaster OX=7227 GN=nAChRbeta1 PE=2 SV=1)

HSP 1 Score: 328.946 bits (842), Expect = 5.549e-104
Identity = 197/335 (58.81%), Postives = 255/335 (76.12%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSP-ETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
             ++ E +L+  L   + YN LIRPV N++  + V   LA +Q+I V EK+Q+MKSNVW+RL W DYQL+W+ +D+G + V R+ P +VW+PDIVLFNNADGNYEV ++ NVL+ P G VLW+PPAI+ SSCTI V YFPFDQQ+C MKFGSWTF+  Q++L   + K  VDLSDYWKSG+W +++ P+ ++   G    P ET I + II++RK+LFYTVNLI+P VLIS L V VFYLPA+AGEK+TL ISILLSLVVFLLL+S+ILPPTS  +PLI+K+LLFTFIMN  +I +TV+IINWNFR P TH+M  +IR  F+  LP FL M+RP +
Sbjct:   24 ASEDEERLVRDLF--RGYNKLIRPVQNMTQKVGVRFGLAFVQLINVNEKNQVMKSNVWLRLVWYDYQLQWDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKSNVLIYPTGEVLWVPPAIYQSSCTIDVTYFPFDQQTCIMKFGSWTFNGDQVSLALYNNKNFVDLSDYWKSGTWDIIEVPAYLNVYEGDSNHPTETDITFYIIIRRKTLFYTVNLILPTVLISFLCVLVFYLPAEAGEKVTLGISILLSLVVFLLLVSKILPPTSLVLPLIAKYLLFTFIMNTVSILVTVIIINWNFRGPRTHRMPMYIRSIFLHYLPAFLFMKRPRK 356          

HSP 2 Score: 79.337 bits (194), Expect = 3.670e-14
Identity = 39/80 (48.75%), Postives = 52/80 (65.00%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSA 1945
            +S     A  A+ FI+EHL  E+   +   DWKYVA V DR+QL IF IVT AGT+GIL+ A +I EYVD++ +I+ Y  
Sbjct:  441 LSPEASKATEAVEFIAEHLRNEDLYIQTREDWKYVAMVIDRLQLYIFFIVTTAGTVGILMDAPHIFEYVDQDRIIEIYRG 520          
BLAST of Acetylcholine receptor subunit beta-like protein vs. UniProt/SwissProt
Match: sp|Q93149|ACR3_CAEEL (Acetylcholine receptor subunit beta-type acr-3 OS=Caenorhabditis elegans OX=6239 GN=acr-3 PE=1 SV=1)

HSP 1 Score: 271.166 bits (692), Expect = 2.717e-82
Identity = 178/352 (50.57%), Postives = 247/352 (70.17%), Query Frame = 2
Query:  194 IFILQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNT-IRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            IF++  LQ     Y N      E +L+ Y+ +   YN LIRPV N S+  I V  +LA + +I V+EK+QIM++NVW  +KW DYQ++W+P +F  +   R+ P +VW PDIVLFNNADGNY VS+  NV+V   G++LW+PPA++ SSC I VE+FPFD+Q C + FGSWTF   +L L Y+ GK+HV+L+DY  SG W ++D P       G      ++I Y I ++RK+LFYTV LI+P VL++ L++ VFYLPA++ EK+TL+ISILL+LVVFLL++S+ILPPTSS+IPL++K+LL TFIMN+ TI ++V+IIN  FR P TH M  W++  F+  LP   +M RPE
Sbjct:   12 IFLITELQC----YPN----SAEERLLSYIFDG--YNSLIRPVLNASSPPIEVFFSLAFVLLINVDEKNQIMQTNVWPTMKWNDYQMQWDPREFDGIKTIRVPPDKVWLPDIVLFNNADGNYLVSFYSNVVVEHTGDMLWVPPAVYKSSCLIDVEFFPFDEQVCSLTFGSWTFRKDELQLSYLSGKRHVELNDYLPSGVWDLIDAP-------GLLIDERSKISYQIKIRRKALFYTVILIMPTVLMAFLSMMVFYLPAESSEKITLAISILLALVVFLLVVSKILPPTSSTIPLMAKYLLMTFIMNMITIMVSVIIINVYFRGPATHIMPNWVKTVFLKFLPVLFVMRRPE 346          

HSP 2 Score: 64.6994 bits (156), Expect = 1.216e-9
Identity = 40/110 (36.36%), Postives = 61/110 (55.45%), Query Frame = 2
Query: 1637 SANDAFFDQRKDFRRNIYPKQL----------KVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKK 1936
            SA   FF +R D + +  PKQ           K+S+  + AI AI +I+ HL  +   K+   +WK+V+ V DR+ L +F  VT  GT+GILL A  + E V++  VI++
Sbjct:  367 SALKTFF-KRNDAKISEQPKQTSRKDGSSSEEKLSSDAKKAIEAIEYITTHLTHDNAFKRQREEWKFVSVVIDRLLLYLFFAVTTGGTVGILLSAPNVFEQVNQTSVIER 475          
BLAST of Acetylcholine receptor subunit beta-like protein vs. UniProt/SwissProt
Match: sp|P30926|ACHB4_HUMAN (Neuronal acetylcholine receptor subunit beta-4 OS=Homo sapiens OX=9606 GN=CHRNB4 PE=1 SV=2)

HSP 1 Score: 267.314 bits (682), Expect = 1.298e-80
Identity = 168/347 (48.41%), Postives = 236/347 (68.01%), Query Frame = 2
Query:  260 ESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPE-TQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRT 1297
            E KL+  LL   +YN LIRP ++ S  I ++L L++ Q+I+V E+ QIM +NVW++ +WTDY+L W  S +  V++ RI   R+W PDIVL+NNADG YEVS   N++V  NG+VLW+PPAI+ S+C I V+YFPFDQQ+C +KF SWT+D +++ +  +     +D  D+  SG W +V  P R    T   Q P    + Y  I++RK LFYT+NLIIPCVL +LL + VFYLP+D GEKMTL IS+LL+L  FLLLIS+I+PPTS  +PLI K+L+FT ++  F+I  +V ++N + RSP+TH M+ W+++ F+  LP FL M+RP    S +   P  KS  T
Sbjct:   27 EEKLMDDLLNKTRYNNLIRPATSSSQLISIKLQLSLAQLISVNEREQIMTTNVWLKQEWTDYRLTWNSSRYEGVNILRIPAKRIWLPDIVLYNNADGTYEVSVYTNLIVRSNGSVLWLPPAIYKSACKIEVKYFPFDQQNCTLKFRSWTYDHTEIDMVLMTPTASMD--DFTPSGEWDIVALPGRR---TVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLTTLLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMAPWVKRCFLHKLPTFLFMKRPGPDSSPARAFPPSKSCVT 368          

HSP 2 Score: 58.151 bits (139), Expect = 1.715e-7
Identity = 24/70 (34.29%), Postives = 45/70 (64.29%), Query Frame = 2
Query: 1700 LKVSNRMR----SAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            L+ S R R     A+  ++FI++H++ ++ ++ ++ DWKYVA V DR+ L +F  V + GT+G+ L  ++
Sbjct:  414 LRSSGRFRQDVQEALEGVSFIAQHMKNDDEDQSVVEDWKYVAMVVDRLFLWVFMFVCVLGTVGLFLPPLF 483          
BLAST of Acetylcholine receptor subunit beta-like protein vs. UniProt/SwissProt
Match: sp|P09484|ACHB2_CHICK (Neuronal acetylcholine receptor subunit beta-2 OS=Gallus gallus OX=9031 GN=CHRNB2 PE=1 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 6.935e-80
Identity = 158/342 (46.20%), Postives = 234/342 (68.42%), Query Frame = 2
Query:  224 VANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
            +A  +  + T  E +L+ YLL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P DF N+   R+    +W PD+VL+NNADG YEVS+  N +++ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D +++ L  +   +   L D+  SG W +V  P R +     D S    I Y  I++RK LFYT+NLIIPC+LI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M  W+R  F+  LP  L M++P++
Sbjct:   10 LAALRRSLCTDTEERLVEYLLDPTRYNKLIRPATNGSQLVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVISYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL--VLKSEVASLDDFTPSGEWDIVALPGRRNE--NPDDSTYVDITYDFIIRRKPLFYTINLIIPCILITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMPPWVRTLFLRKLPALLFMKQPQQ 347          

HSP 2 Score: 58.9214 bits (141), Expect = 1.041e-7
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 2
Query: 1718 MRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            +  A+  + FI++H+  E++++ +  DWKYVA V DR+ L IF  V + GT+G+ L+ ++
Sbjct:  413 LEEAVEGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTVGMFLQPLF 472          
BLAST of Acetylcholine receptor subunit beta-like protein vs. UniProt/SwissProt
Match: sp|P12390|ACHB2_RAT (Neuronal acetylcholine receptor subunit beta-2 OS=Rattus norvegicus OX=10116 GN=Chrnb2 PE=1 SV=2)

HSP 1 Score: 263.077 bits (671), Expect = 5.780e-79
Identity = 156/336 (46.43%), Postives = 232/336 (69.05%), Query Frame = 2
Query:  245 VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERK 1252
            +GT  E +L+ +LL+  +YN LIRP +N S  + V+L +++ Q+I+V E+ QIM +NVW+  +W DY+L W+P DF N+   R+    +W PD+VL+NNADG YEVS+  N +V+ +G++ W+PPAI+ S+C I V++FPFDQQ+C MKF SWT+D +++ L  +       L D+  SG W ++  P R +     D S    I Y  I++RK LFYT+NLIIPCVLI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PL+ K+L+FT ++  F+I  +V ++N + RSP TH M+ W++  F++ LP  L +++P  +
Sbjct:   23 LGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL--VLKSDVASLDDFTPSGEWDIIALPGRRNE--NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPTLLFLQQPRHR 354          

HSP 2 Score: 63.5438 bits (153), Expect = 3.094e-9
Identity = 32/108 (29.63%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 1574 RSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            R G     C  F  P+S   ++             R++ P     S  +R A+  + FI++H+  E++++ +  DWKYVA V DR+ L IF  V + GT+G+ L+ ++
Sbjct:  378 REGPAADPCTCFVNPASVQGLAGAFRAEPTAAGPGRSVGP----CSCGLREAVDGVRFIADHMRSEDDDQSVREDWKYVAMVIDRLFLWIFVFVCVFGTVGMFLQPLF 481          
BLAST of Acetylcholine receptor subunit beta-like protein vs. TrEMBL
Match: A0A267DUG9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019166g1 PE=3 SV=1)

HSP 1 Score: 399.823 bits (1026), Expect = 1.154e-128
Identity = 230/355 (64.79%), Postives = 287/355 (80.85%), Query Frame = 2
Query:  194 IFILQHLQNSV-ANYQNR---VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            + I+Q L   V   YQ R   +G+  E KL++ L +HK YN LIRPVSNL+ T++V   LAMIQ+I VEEKSQ+MKSNVW+R+ W+DYQLRW+PSDFG + V R+ P+++W+PDIVL+NNADG YEVSWQPN+L+  +G +LW+PPAI+ SSCTIHV+YFPFDQQ CEMKFGSWTFDA+Q+ L + +G +  DL+DYWKSGSW ++DCP  +  +    Q P+T +++TI ++RK+LFYTVNLIIPCVLIS L+VCVFYLPADAGEKMTL ISILL+LVVFLLL+S+ILPPTS SIPLISKFLLFTFIMNI TI  TV+IINWNFR+P THKM  W+R  F+  LP+ L M+RPE
Sbjct:    8 LLIVQFLSCLVRPTYQVRESGIGSDDEIKLVNLLFKHKGYNRLIRPVSNLNQTVQVGFGLAMIQLINVEEKSQVMKSNVWLRMTWSDYQLRWDPSDFGGIQVIRVNPNKIWKPDIVLYNNADGRYEVSWQPNILIFSSGTILWVPPAIYKSSCTIHVQYFPFDQQECEMKFGSWTFDATQVVLDWYEGARVADLNDYWKSGSWDIIDCPGNITLVEKLGQPPQTMMIFTITIRRKTLFYTVNLIIPCVLISFLSVCVFYLPADAGEKMTLCISILLALVVFLLLVSKILPPTSISIPLISKFLLFTFIMNIITIVFTVIIINWNFRTPRTHKMPNWVRLLFLKYLPRVLFMKRPE 362          

HSP 2 Score: 98.2117 bits (243), Expect = 9.550e-18
Identity = 45/84 (53.57%), Postives = 67/84 (79.76%), Query Frame = 2
Query: 1700 LKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSAAS 1951
            L+V+  +R AI+AINFIS HL +E+  K++++DWKYVA+V DRIQLVIF+ VTI GT+ IL+ A +IL++VD++ +IK+ +  S
Sbjct:  452 LEVTPDLRRAIAAINFISTHLRIEDEYKRVLQDWKYVASVIDRIQLVIFSSVTIVGTVAILMNAPFILDFVDQDAIIKRLTDKS 535          
BLAST of Acetylcholine receptor subunit beta-like protein vs. TrEMBL
Match: A0A1I8GCT6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 397.897 bits (1021), Expect = 2.025e-128
Identity = 223/334 (66.77%), Postives = 278/334 (83.23%), Query Frame = 2
Query:  245 VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            +G+  E KL++ L +HK YN LIRPVSNL+ T++V   LAMIQ+I VEEKSQ+MKSNVW+R+ W+DYQLRW+PSDFG + V R+ P+++W+PDIVL+NNADG YEVSWQPN+L+  +G +LW+PPAI+ SSCTIHV+YFPFDQQ CEMKFGSWTFDA+Q+ L + +G +  DL+DYWKSGSW ++DCP  +  +    Q P+T +++TI ++RK+LFYTVNLIIPCVLIS L+VCVFYLPADAGEKMTL ISILL+LVVFLLL+S+ILPPTS SIPLISKFLLFTFIMNI TI  TV+IINWNFR+P THKM  W+R  F+  LP+ L M+RPE
Sbjct:    1 IGSDDEIKLVNLLFKHKGYNRLIRPVSNLNQTVQVGFGLAMIQLINVEEKSQVMKSNVWLRMTWSDYQLRWDPSDFGGIQVIRVNPNKIWKPDIVLYNNADGRYEVSWQPNILIFSSGTILWVPPAIYKSSCTIHVQYFPFDQQECEMKFGSWTFDATQVVLDWYEGARVADLNDYWKSGSWDIIDCPGNITLVEKLGQPPQTMMIFTITIRRKTLFYTVNLIIPCVLISFLSVCVFYLPADAGEKMTLCISILLALVVFLLLVSKILPPTSISIPLISKFLLFTFIMNIITIVFTVIIINWNFRTPRTHKMPNWVRLLFLKYLPRVLFMKRPE 334          

HSP 2 Score: 98.2117 bits (243), Expect = 8.134e-18
Identity = 45/84 (53.57%), Postives = 67/84 (79.76%), Query Frame = 2
Query: 1700 LKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSAAS 1951
            L+V+  +R AI+AINFIS HL +E+  K++++DWKYVA+V DRIQLVIF+ VTI GT+ IL+ A +IL++VD++ +IK+ +  S
Sbjct:  423 LEVTPDLRRAIAAINFISTHLRIEDEYKRVLQDWKYVASVIDRIQLVIFSSVTIVGTVAILMNAPFILDFVDQDAIIKRLTDKS 506          
BLAST of Acetylcholine receptor subunit beta-like protein vs. TrEMBL
Match: A0A1I8HFW9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 399.438 bits (1025), Expect = 2.737e-128
Identity = 223/338 (65.98%), Postives = 279/338 (82.54%), Query Frame = 2
Query:  233 YQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            +   +G+  E KL++ L +HK YN LIRPVSNL+ T++V   LAMIQ+I VEEKSQ+MKSNVW+R+ W+DYQLRW+PSDFG + V R+ P+++W+PDIVL+NNADG YEVSWQPN+L+  +G +LW+PPAI+ SSCTIHV+YFPFDQQ CEMKFGSWTFDA+Q+ L + +G +  DL+DYWKSGSW ++DCP  +  +    Q P+T +++TI ++RK+LFYTVNLIIPCVLIS L+VCVFYLPADAGEKMTL ISILL+LVVFLLL+S+ILPPTS SIPLISKFLLFTFIMNI TI  TV+IINWNFR+P THKM  W+R  F+  LP+ L M+RPE
Sbjct:   28 FATGIGSDDEIKLVNLLFKHKGYNRLIRPVSNLNQTVQVGFGLAMIQLINVEEKSQVMKSNVWLRMTWSDYQLRWDPSDFGGIQVIRVNPNKIWKPDIVLYNNADGRYEVSWQPNILIFSSGTILWVPPAIYKSSCTIHVQYFPFDQQECEMKFGSWTFDATQVVLDWYEGARVADLNDYWKSGSWDIIDCPGNITLVEKLGQPPQTMMIFTITIRRKTLFYTVNLIIPCVLISFLSVCVFYLPADAGEKMTLCISILLALVVFLLLVSKILPPTSISIPLISKFLLFTFIMNIITIVFTVIIINWNFRTPRTHKMPNWVRLLFLKYLPRVLFMKRPE 365          

HSP 2 Score: 97.8265 bits (242), Expect = 1.464e-17
Identity = 44/81 (54.32%), Postives = 66/81 (81.48%), Query Frame = 2
Query: 1700 LKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYS 1942
            L+V+  +R AI+AINFIS HL +E+  K++++DWKYVA+V DRIQLVIF+ VTI GT+ IL+ A +IL++VD++ +IK+ +
Sbjct:  454 LEVTPDLRRAIAAINFISTHLRIEDEYKRVLQDWKYVASVIDRIQLVIFSSVTIVGTVAILMNAPFILDFVDQDAIIKRLT 534          
BLAST of Acetylcholine receptor subunit beta-like protein vs. TrEMBL
Match: A0A1I8H157 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 397.127 bits (1019), Expect = 1.186e-127
Identity = 223/334 (66.77%), Postives = 278/334 (83.23%), Query Frame = 2
Query:  245 VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            +G+  E KL++ L +HK YN LIRPVSNL+ T++V   LAMIQ+I VEEKSQ+MKSNVW+R+ W+DYQLRW+PSDFG + V R+ P+++W+PDIVL+NNADG YEVSWQPN+L+  +G +LW+PPAI+ SSCTIHV+YFPFDQQ CEMKFGSWTFDA+Q+ L + +G +  DL+DYWKSGSW ++DCP  +  +    Q P+T +++TI ++RK+LFYTVNLIIPCVLIS L+VCVFYLPADAGEKMTL ISILL+LVVFLLL+S+ILPPTS SIPLISKFLLFTFIMNI TI  TV+IINWNFR+P THKM  W+R  F+  LP+ L M+RPE
Sbjct:    1 IGSDDEIKLVNLLFKHKGYNRLIRPVSNLNQTVQVGFGLAMIQLINVEEKSQVMKSNVWLRMTWSDYQLRWDPSDFGGIQVIRVNPNKIWKPDIVLYNNADGRYEVSWQPNILIFSSGTILWVPPAIYKSSCTIHVQYFPFDQQECEMKFGSWTFDATQVVLDWYEGARVADLNDYWKSGSWDIIDCPGNITLVEKLGQPPQTMMIFTITIRRKTLFYTVNLIIPCVLISFLSVCVFYLPADAGEKMTLCISILLALVVFLLLVSKILPPTSISIPLISKFLLFTFIMNIITIVFTVIIINWNFRTPRTHKMPNWVRLLFLKYLPRVLFMKRPE 334          

HSP 2 Score: 97.8265 bits (242), Expect = 1.559e-17
Identity = 44/81 (54.32%), Postives = 66/81 (81.48%), Query Frame = 2
Query: 1700 LKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYS 1942
            L+V+  +R AI+AINFIS HL +E+  K++++DWKYVA+V DRIQLVIF+ VTI GT+ IL+ A +IL++VD++ +IK+ +
Sbjct:  424 LEVTPDLRRAIAAINFISTHLRIEDEYKRVLQDWKYVASVIDRIQLVIFSSVTIVGTVAILMNAPFILDFVDQDAIIKRLT 504          
BLAST of Acetylcholine receptor subunit beta-like protein vs. TrEMBL
Match: A0A564Z310 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS12072 PE=3 SV=1)

HSP 1 Score: 399.823 bits (1026), Expect = 2.154e-127
Identity = 249/575 (43.30%), Postives = 375/575 (65.22%), Query Frame = 2
Query:  236 QNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLSQXXXXXXXXXXXXXXLFANPWNNYQNMSRHSLDSESDKIPDSKRTKCTCSASTNN--IDRSGTDKTHCDTFECPSSPSDI---SANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSA 1945
            Q ++ ++ E +L +YL   + YNPLIRPV+N+S  +RV+L L MI +I ++E+ Q+MKSNVW+ + W DYQL W+P+ +G + V R+  SRVW+PDIVLFNNADGNYEV WQPN+LV  +G++LW+PPA++ SSC I VEYFPFD+Q CEMKFGSW FDAS++++G+    + +DLSDYW+SG+W +VD P+R+ N +  + +  T IVYTI L+RK+LFY+VNLI+PCV++SLL+V VFYLP+DAGEK+TL+ISI+++LVVFL+L+S+ILPPTS+++PLIS++L+FTF+ +I  + ++V+++NWN+RSP TH M  WI+  F+DVLP+FL M R E  YS         S  T+          Y + N  AF+    F   +   L          +   ++   N  + Y+N++   L   + +  DS ++  + + S +   I  +G+ K      +   SP  I   S       + KD   +     +     +  A+ AI +ISE LE +E ++ ++++WKY A+V DR+ LVIFT+V++   + +L  A  +L  +D++ VI KYS 
Sbjct:   73 QTKIASEQEYELTNYLFNEQGYNPLIRPVANVSEALRVDLGLCMIHLIHIDERRQVMKSNVWLPMVWRDYQLTWDPAKYGGLKVIRVPHSRVWKPDIVLFNNADGNYEVLWQPNLLVHHDGSILWMPPAVYESSCAIDVEYFPFDEQECEMKFGSWAFDASRISMGFYSSLEFMDLSDYWRSGTWDLVDVPARIVNTSNGEVN-NTYIVYTIKLRRKTLFYSVNLIVPCVMLSLLSVFVFYLPSDAGEKVTLAISIVVALVVFLILVSKILPPTSTTVPLISRYLMFTFVSDILAVFMSVLVVNWNYRSPRTHTMPKWIKTVFLDVLPRFLFMNRLEHLYSKDYDSDFPSSYFTINK--------YSSENEDAFI--QEFPEGASLDLISSVSTDSEESRPKSVSTFNRGHFYENLTAQRLSVSTGRPFDSSKSDESSTTSDDGEIIVLNGSLKLR----KMKHSPHRIGIRSRRMGITCEGKDDNSSAQKGVIGGGFALTEAVKAIEYISERLEKKEKDRIVVQEWKYAASVIDRLVLVIFTVVSVVVALSLLTNAPSLLSPIDQDAVINKYSV 632          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Cavefish
Match: chrna4b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:103025036])

HSP 1 Score: 283.878 bits (725), Expect = 4.832e-87
Identity = 206/559 (36.85%), Postives = 318/559 (56.89%), Query Frame = 2
Query:  260 ESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPET--QIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE-------RKYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLSQXXXXXXXXXXXXXXLFANPWNNYQNMSRHSLDS----ESDKIPDSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPK----QLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILL 1885
            E +L+  L    +YN L RPV+N+S+ + V   L++ Q+I V+EK+Q+M +NVW++ +W+DY+LRW P ++ NV   RI    +W+PDIVL+NNADG++ V+      +  +G + W PPAI+ SSC+I V +FPFDQQ+C+MKFGSWT+D +++ L  +D    VD  DYW+SG W +++   +  N    +   E    I Y+ I++R  LFYT+NLIIPC+LIS LTV VFYLP++  EK+TL IS+LLSL VFLLLI+ I+P TS  IPLI ++LLFT I    +I ITV ++N + RS  TH+M  W+R+ F+ ++P++L M+RP        RK      +P +    +  S  +I     +   P+    +P        + S  +++        ++L   P      + RHS +S      + +P+++  +  C             KTHC   E   S    S ++    Q      +  PK    ++ +S  +  A+  + +I++HL  E+++  +  DWKYVA V DRI L +F +V I GT G+ L
Sbjct:   28 EERLLQVLF--SRYNKLSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWSDYKLRWNPEEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAQLFHDGRIKWKPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDRAKIDLVSMDDD--VDQMDYWESGEWVIINAVGKY-NTKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSRTHRMPHWVRQLFLQLVPRYLFMKRPPASGKRNCRKLIEMMHKPVMPQAISPSSQYSI-----LQEEPTQAPQTPELRFPPTHNSSPSSLSLSPDTPLGSLLHTLPLTEICELYRHSAESVFVDSKEGLPETETLR-HCRK-----HEGAAIKTHC-IAEGGGSTGQGSRHNLLDTQNSSNNCSKEPKTEEVEIPISQSLLRALEGVQYIADHLRAEDSDFSVREDWKYVAMVIDRIFLWMFVLVCILGTAGLFL 569          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Cavefish
Match: chrna3 (neuronal acetylcholine receptor subunit alpha-3-like [Source:NCBI gene;Acc:103025148])

HSP 1 Score: 267.314 bits (682), Expect = 4.698e-82
Identity = 159/335 (47.46%), Postives = 232/335 (69.25%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
             ++ E +L   +     YN  IRPV N+S+ + V+  ++M Q++ V+E +QIM++N+W+R  W DY+LRW+P DFG V+  R+  +R+W+PDIVL+NNA G+++V  +   L+  NG+V WIPPAIF SSC I V YFPFD Q+C MKFGSWT+D +++ L  I     ++L D+W+SG W ++D P    +I     +   T I Y++ ++R  LFYT+N+IIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W+R+ F+ +LP+ + M RP+R
Sbjct:   21 ASEAEHRLFSVIF--SSYNQYIRPVENVSDPVIVQFEVSMSQLVKVDEVNQIMETNLWLRHIWNDYKLRWDPKDFGGVEFIRVPSNRIWKPDIVLYNNAVGDFQVDDKTKALLRYNGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLIGST--INLKDFWESGEWMIIDAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPCWVRRVFLGLLPRVMFMTRPDR 351          

HSP 2 Score: 60.8474 bits (146), Expect = 2.731e-9
Identity = 26/67 (38.81%), Postives = 45/67 (67.16%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILE 1906
            +S  +R AI ++ +I+E++ ++   K++  DWKYVA V DRI L +F +V I GT G+ L+ + ++E
Sbjct:  393 LSPEVREAIESVKYIAENMRLQNEAKEVQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLLLVE 459          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Cavefish
Match: chrna4b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:103025036])

HSP 1 Score: 269.24 bits (687), Expect = 5.536e-81
Identity = 206/597 (34.51%), Postives = 322/597 (53.94%), Query Frame = 2
Query:  260 ESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPET--QIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFL------------------MMERPERKYSMS---------------------------NKEPNVKSVRTVKSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLSQXXXXXXXXXXXXXXLFANPWNNYQNMSRHSLDS----ESDKIPDSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPK----QLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILL 1885
            E +L+  L    +YN L RPV+N+S+ + V   L++ Q+I V+EK+Q+M +NVW++ +W+DY+LRW P ++ NV   RI    +W+PDIVL+NNADG++ V+      +  +G + W PPAI+ SSC+I V +FPFDQQ+C+MKFGSWT+D +++ L  +D    VD  DYW+SG W +++   +  N    +   E    I Y+ I++R  LFYT+NLIIPC+LIS LTV VFYLP++  EK+TL IS+LLSL VFLLLI+ I+P TS  IPLI ++LLFT I    +I ITV ++N + RS  TH+M  W+R+ F+ ++P++L                  MM +P    +M                             +EP  K+     S ++ +++  +   P+    +P        + S  +++        ++L   P      + RHS +S      + +P+++  +  C             KTHC   E   S    S ++    Q      +  PK    ++ +S  +  A+  + +I++HL  E+++  +  DWKYVA V DRI L +F +V I GT G+ L
Sbjct:   31 EERLLQVLF--SRYNKLSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWSDYKLRWNPEEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAQLFHDGRIKWKPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDRAKIDLVSMDDD--VDQMDYWESGEWVIINAVGKY-NTKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSRTHRMPHWVRQLFLQLVPRYLFMKRPPASGKRNCRKLIEMMHKPVMPQAMLLGATTLPPSENPCPDPDGVSAAQSGGLQREPASKTPLFCSSPSSQYSI--LQEEPTQAPQTPELRFPPTHNSSPSSLSLSPDTPLGSLLHTLPLTEICELYRHSAESVFVDSKEGLPETETLR-HCRK-----HEGAAIKTHC-IAEGGGSTGQGSRHNLLDTQNSSNNCSKEPKTEEVEIPISQSLLRALEGVQYIADHLRAEDSDFSVREDWKYVAMVIDRIFLWMFVLVCILGTAGLFL 613          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Cavefish
Match: chrna3 (neuronal acetylcholine receptor subunit alpha-3-like [Source:NCBI gene;Acc:103025148])

HSP 1 Score: 266.929 bits (681), Expect = 5.669e-81
Identity = 159/335 (47.46%), Postives = 232/335 (69.25%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER 1249
             ++ E +L   +     YN  IRPV N+S+ + V+  ++M Q++ V+E +QIM++N+W+R  W DY+LRW+P DFG V+  R+  +R+W+PDIVL+NNA G+++V  +   L+  NG+V WIPPAIF SSC I V YFPFD Q+C MKFGSWT+D +++ L  I     ++L D+W+SG W ++D P    +I     +   T I Y++ ++R  LFYT+N+IIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W+R+ F+ +LP+ + M RP+R
Sbjct:   18 ASEAEHRLFSVIFS--SYNQYIRPVENVSDPVIVQFEVSMSQLVKVDEVNQIMETNLWLRHIWNDYKLRWDPKDFGGVEFIRVPSNRIWKPDIVLYNNAVGDFQVDDKTKALLRYNGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLIGST--INLKDFWESGEWMIIDAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPCWVRRVFLGLLPRVMFMTRPDR 348          

HSP 2 Score: 61.2326 bits (147), Expect = 1.828e-9
Identity = 26/67 (38.81%), Postives = 45/67 (67.16%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILE 1906
            +S  +R AI ++ +I+E++ ++   K++  DWKYVA V DRI L +F +V I GT G+ L+ + ++E
Sbjct:  473 LSPEVREAIESVKYIAENMRLQNEAKEVQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLLLVE 539          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Cavefish
Match: chrna6 (neuronal acetylcholine receptor subunit alpha-3 [Source:NCBI gene;Acc:103022127])

HSP 1 Score: 253.062 bits (645), Expect = 8.918e-77
Identity = 151/325 (46.46%), Postives = 223/325 (68.62%), Query Frame = 2
Query:  299 YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNK 1270
            YN  IRPV N+S+ + VE  +++ Q++ V+E +QIM++N+W+R  W DY+L+W P++F  ++  R+  +++W+PDIVL+NNA G++ V  +   L+  +G + W+PPAIF SSC + + YFPFD Q+C MKFGSWT+D +++ L  I  K  V+L D+W+SG W ++D P    +I     +     I Y+  ++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M GW+R  F+  LP+ ++M RP  +   S K
Sbjct:   28 YNQFIRPVENVSDPVTVEFEVSISQLVKVDEVNQIMETNLWLRHIWNDYKLKWIPAEFDGIEYIRVPSNKIWRPDIVLYNNAVGDFLVEDKTKALLKFDGTITWVPPAIFKSSCPMDITYFPFDYQNCSMKFGSWTYDKAKIDLVLIGSK--VNLKDFWESGEWEIIDAPGYKHDIKYNCCEEIYPDITYSFYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPGWVRTVFLRALPRIMLMRRPIDQSESSTK 350          

HSP 2 Score: 53.9138 bits (128), Expect = 3.501e-7
Identity = 23/63 (36.51%), Postives = 40/63 (63.49%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAV 1894
            VS  ++ AI ++ +I+E++      K++  DWKYVA V DRI L +F +V + GT+G+  + +
Sbjct:  380 VSPEIKQAIESVKYIAENMRSRNKAKEVEDDWKYVAMVIDRIFLWVFVLVCVLGTVGLFFQPL 442          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Sea Lamprey
Match: chrna3 (cholinergic receptor, nicotinic, alpha 3 [Source:ZFIN;Acc:ZDB-GENE-070822-1])

HSP 1 Score: 253.447 bits (646), Expect = 4.289e-77
Identity = 147/317 (46.37%), Postives = 221/317 (69.72%), Query Frame = 2
Query:  299 YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            YNP IRPV N+S+ + V   ++M Q++ ++E +QIM +N+W++  W DY+ +W P  F  ++  R+   ++W+PDIVL+NNADG+++V  +   L+  +G V+W+PPAIF SSC I V YFPFD Q+C MKFGSWT+D +++ L  +  K  ++L D+W+SG W ++D P    +I     +     I Y++ ++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TLSIS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I IT+ ++N ++R+P  H M  W+R+ F+D LPK ++++ P+
Sbjct:   19 YNPYIRPVFNVSDPVIVRFEVSMSQLVKLDEVNQIMATNLWLKHTWNDYKFQWNPGQFEGMEYIRVPSDKIWKPDIVLYNNADGDFQVEGKTKALLKYDGTVMWMPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLVGTK--INLKDFWESGEWVIIDVPGYKHDIKYNCCEQIYPDITYSVYVRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLSISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITIFVLNVHYRTPEAHTMPNWVRRVFLDTLPKIMLLQPPK 333          

HSP 2 Score: 56.9954 bits (136), Expect = 7.949e-9
Identity = 25/60 (41.67%), Postives = 39/60 (65.00%), Query Frame = 2
Query: 1709 SNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLK 1888
            S  +R  I ++ +I+E+L  ++  K++  DWKYVA V DRI L +F +V I GT G+ L+
Sbjct:  425 SPEIRQVIDSVKYIAENLRTQDEAKEVCDDWKYVAMVVDRIFLWVFVLVCILGTAGLFLQ 484          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Sea Lamprey
Match: chrnb3b (cholinergic receptor, nicotinic, beta 3b (neuronal) [Source:ZFIN;Acc:ZDB-GENE-030102-4])

HSP 1 Score: 222.631 bits (566), Expect = 7.090e-66
Identity = 145/342 (42.40%), Postives = 219/342 (64.04%), Query Frame = 2
Query:  251 TQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPS-RMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHK-MSGWIRKWFIDVLPKFLMMERPERKYSMSNK 1270
             + E +L+  +L  K Y   +RPV  LS+T+ V+  L + Q++ V+EK+Q+M +NVW++ +W D  LRW P D+G ++  R+  S +W PDIVL++NADG +E S     +V  +G + W PPAI+ SSCTI V +FPFDQQ+C MKFGSWT+D + + L  ++    VD  +Y+ +G W  ++    +   + G    P   I Y+ +++R  LFYT+ LI+PC+ +S LTV VFYLP+D GEK++L  S+L+SL VFLL+I  I+P +S  IPLI ++LLF  I    +I ITV +IN + RS +TH  M+ W+R+ F+  LPK L M     +Y+  ++
Sbjct:   32 AELEDRLLKRML--KDYQKWVRPVDKLSDTVEVKFGLGIAQLVDVDEKNQLMTTNVWLKQEWVDINLRWNPDDYGGINSIRVPASSIWLPDIVLYDNADGRFEGSIVTKAVVKHDGRIKWTPPAIYKSSCTIDVTFFPFDQQNCSMKFGSWTYDGTLVDLIMVNSD--VDRGEYFDNGEWEFLNATGNKGVRLDGHRFYP--YITYSFVIRRLPLFYTLFLIVPCLGLSFLTVFVFYLPSDEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIITVFVINVHHRSSSTHHPMAPWVRQLFLHKLPKILCMRSHVDRYTAPDE 367          

HSP 2 Score: 50.0618 bits (118), Expect = 1.220e-6
Identity = 21/58 (36.21%), Postives = 38/58 (65.52%), Query Frame = 2
Query: 1724 SAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            +AI ++ +I+ H++ E   ++++ DWK+VA V DRI L  F  V++ G++ I L  +Y
Sbjct:  396 TAIDSVRYITRHIKKENEVREVVEDWKFVAQVVDRILLWTFLSVSVFGSILIFLPTIY 453          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Sea Lamprey
Match: chrna2a (cholinergic receptor, nicotinic, alpha 2a (neuronal) [Source:ZFIN;Acc:ZDB-GENE-040108-2])

HSP 1 Score: 221.09 bits (562), Expect = 2.848e-64
Identity = 145/293 (49.49%), Postives = 202/293 (68.94%), Query Frame = 2
Query:  371 QIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPET--QIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
             +   +EK+Q+M +NVW++ +W DY+L+W+P+++ NV   R+    +W PDIVL+NNADG++ V+      +   G V W PPAI+ SSC+I V +FPFDQQ+C MKFGSWT+D +++ L  +   + VDL DYW+SG W +V       N    D  P+    I Y+ I++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLLLI+ I+P TS  IPLI ++LLFT I    +I ITV ++N + RSP TH M  W+R+ F+D +P++L+M RP
Sbjct:    9 HLCDCDEKNQMMTTNVWLKQEWDDYKLQWDPAEYENVTSIRVPSELIWVPDIVLYNNADGDFAVTHMTKAHLFHTGRVRWKPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDL--VSMARDVDLKDYWESGEWAIVHAVGTY-NTKKYDCCPQVYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPATHAMPRWVRRVFLDRVPRWLVMRRP 298          

HSP 2 Score: 53.1434 bits (126), Expect = 1.519e-7
Identity = 21/52 (40.38%), Postives = 33/52 (63.46%), Query Frame = 2
Query: 1730 ISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILL 1885
            +  + +I+ HL  E+ +  +  DWKYVA V DRI L +F +V + GT+G+ L
Sbjct:  507 VQGVQYIANHLRAEDAHLSVKEDWKYVAMVIDRIFLWVFIVVCLLGTVGLFL 558          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Sea Lamprey
Match: chrnb1l (cholinergic receptor, nicotinic, beta 1 (muscle) like [Source:ZFIN;Acc:ZDB-GENE-060811-1])

HSP 1 Score: 209.534 bits (532), Expect = 1.117e-60
Identity = 149/394 (37.82%), Postives = 229/394 (58.12%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGY-IDGKKH------VDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNPSAFVY----SPSFLRKSYQS 1396
            G + E  L+  L   +QY  L+RP    +  + V L + + Q+I++      M  +  I L W D +L W+P ++ N+   R++P RVW PDI L NN DGN++     +VLV   G V W+PP+I+ SSC + V+YFPFD Q+C M F S+T+D S++ L Y +D  +       +D S +  SG W +   PSR +  T         I + +I++RK LFY +N+++PC+LI++  V  FYLP DAGEKM L IS+LL+L VFLLL++  +P TS  +P+I  +L+FT ++    + +TV+++N +FRSP TH M  W+R+ FI  LPK+L +ERP +K  ++ K P +      +   + H+  +I   PS  +     SPSF    + S
Sbjct:   20 GLEAEENLLKDLFVVQQYKTLVRPARTATGRVTVRLGMTLSQLISLPACQPDMTLSRLISLSWEDPRLSWDPRNYDNLRSIRLSPKRVWLPDIALSNNNDGNFDFGLYVHVLVDYTGAVRWVPPSIYRSSCGMQVKYFPFDWQNCSMVFQSFTYDTSEINLEYALDEHRQEIRHIIIDESTFPVSGEWQIKHKPSRKN--TNPRDPLYQDITFYLIMERKPLFYIINIVVPCILITICAVFTFYLPPDAGEKMGLCISVLLALTVFLLLLADKVPETSLGVPVIVNYLMFTMVIVTACVILTVIVLNLHFRSPATHIMPYWVRRIFIHSLPKYLWIERP-KKEELAPKLPVL-----TQHQQHCHDEYFIRTPPSQALLLLPTSPSFGMTPFSS 405          

HSP 2 Score: 57.3806 bits (137), Expect = 6.256e-9
Identity = 27/71 (38.03%), Postives = 43/71 (60.56%), Query Frame = 2
Query: 1691 PKQLKV--SNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            P +L V     ++SA+ A+++ISEH+  ++   ++  DW+YVA V DRI  + F ++T  GT GI   A Y
Sbjct:  410 PDKLHVVLPPELQSAVDAVHYISEHMLSQKEFDELKEDWQYVAMVVDRIFFITFFLITSLGTFGIFFNASY 480          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008550.1 (pep scaffold:Pmarinus_7.0:GL476601:34:38373:1 gene:ENSPMAG00000007717.1 transcript:ENSPMAT00000008550.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 154.451 bits (389), Expect = 2.512e-41
Identity = 111/302 (36.75%), Postives = 179/302 (59.27%), Query Frame = 2
Query:  491 RITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNI-TGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPER-------------KYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNPSA 1354
            R  P ++W PDI+L+N+AD  ++ ++  NVLV   GN   +PP +  S+C I V +FPFD Q C++KFGSWT D   + L  ++     D S Y  +G W +V+ P + + +     Q P   + YT+ ++R++L+Y +NL++PCVLIS L++ VF LPAD+GEK++L I++LLSL VF+LL++ I+P TS S+PLI ++   T I+  F++ +TV+++ ++  +P+  KM  W+R   +D    FL M++P R             + SM + +  + S   +    N H V     NP A
Sbjct:   10 RFQPHQIWTPDILLYNSADERFDATFHTNVLVNYTGNCQHLPPGMLKSTCYIDVRWFPFDVQKCDLKFGSWTHDGWLIDLQMLEA----DTSGYIANGEWDLVEIPGKRNELYYDCCQEPYPDVTYTLTIRRRTLYYGLNLLVPCVLISALSLLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIGQYFASTMIIVAFSVVVTVIVLQYHHHNPDGKKMPQWVRVILLDWCAWFLRMKQPGRERHELARRLSILHRNSMVSLDLALLSASGIHKKTNGHGVFIGFTNPDA 307          

HSP 2 Score: 48.9062 bits (115), Expect = 2.652e-6
Identity = 22/72 (30.56%), Postives = 42/72 (58.33%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDEN 1921
            +S+ +   +  + +I+     ++ N+ I  +WK+ AAV DR+ L+ FT+ TI  T+ IL+ A +  E V ++
Sbjct:  350 MSSEITKILIELRYIAGRFRTQDENEAIASEWKFAAAVVDRLCLLAFTLFTIISTISILMSAPHFTEAVAKD 421          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Nematostella
Match: EDO38722 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC04])

HSP 1 Score: 192.971 bits (489), Expect = 5.829e-57
Identity = 96/247 (38.87%), Postives = 147/247 (59.51%), Query Frame = 2
Query:  260 ESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEV--SWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYID-GKKHVDLSDYWKSGSWYMVDCPSRMDNITG-ADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMT 988
            E +L+ +L   K Y+P +RPV   S+T+ V L + + Q+I VE K+Q++KS+++IR  W +  L+W  S FG +    + P R+W+PDI L+NN D        +  +V ++  G  +W+ P I  SSC   V +FPFD+Q C++KFGSWT+D   L +      K   D   + K G W ++  P R + +       P   + YTIIL+RKS+FY VN+I PC LI+ LT+  FYLP+++GE++T
Sbjct:    1 EQRLVDHLF--KGYDPTVRPVYKKSDTVLVRLGITLHQLIDVEFKNQVVKSSIFIRQAWLNPFLKWNASAFGGITTINVDPKRIWKPDIYLYNNVDDQTGAMNQFDTHVTLSFTGQSMWLAPKILKSSCKFDVTHFPFDEQKCDLKFGSWTYDGFHLDIASEHPAKCEGDYKKFIKGGEWDLIGLPCRRNELVYICCPEPYPDLTYTIILRRKSMFYFVNMIAPCFLITALTLLSFYLPSESGERLT 245          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Nematostella
Match: EDO42054 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2J7])

HSP 1 Score: 198.749 bits (504), Expect = 9.959e-57
Identity = 99/255 (38.82%), Postives = 156/255 (61.18%), Query Frame = 2
Query:  236 QNRVGTQHESKLIHYLLE-HKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVS---WQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNI-TGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKM 985
            + R G   E+     L +  + Y+  +RPV N S+ + VE +L++IQIIAV  KSQ++  +VWIR KWT+  L W  S +G ++   + P++VW PDI+L+NNAD  Y      ++ +V+ + +G   W  PA F S+C I+V YFPFD Q+C +KFGSWT+  ++LT+         DLS Y  +G W+++  P++++ +     + P   +   + +QRK L+Y  NLIIPC LI+ LT+  F+LP ++GEK+
Sbjct:   27 RKRRGASRETAEKRLLADLFRNYDSEVRPVFNDSDKVNVEFSLSLIQIIAVNSKSQLVTVSVWIRQKWTNPMLAWNTSKYGGIESVNLNPAKVWIPDIILYNNADETYAGGLEKYKTSVIASSDGTQEWNSPASFTSTCNINVRYFPFDSQNCSLKFGSWTYVGNRLTM--TSTSDSADLSKYVNNGEWHLLAMPAKLNRVFYNCCKYPFYDVTLYLEIQRKPLYYVFNLIIPCALIATLTLIKFFLPPESGEKV 279          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Nematostella
Match: EDO32052 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW62])

HSP 1 Score: 187.963 bits (476), Expect = 1.099e-54
Identity = 87/236 (36.86%), Postives = 147/236 (62.29%), Query Frame = 2
Query:  293 KQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQP---NVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMD-NITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMT 988
            K Y P  RPV ++ + + V++ L + QII V+EK+QI+ +NVWIRL W D+ L W P DFG V    + P+ VW+PDI+L+N  D   E  ++     V+++ +G+  W+ PAI  + C + V  FPFD Q C++KFGSWT D  ++ +  +     VD+ DY ++  W++ D P R D  +      P   + +T++++R+++FY  N+++P  +I+LL++  FYLP ++GE+++
Sbjct:    3 KNYTPEARPVLHVKDPVVVKMGLMLKQIIDVDEKNQILTTNVWIRLYWKDHLLNWNPEDFGGVKSINVEPNNVWKPDILLYNPTDLRVESLYKKIDTKVILSSDGSAAWLSPAIIKTECKVDVRLFPFDVQYCDLKFGSWTHDGLKIDIQ-LRKTTGVDIDDYSENREWFLKDAPGRRDVKLYNCCPEPYPTVTFTLVIRRRAMFYVYNMVLPTGMIALLSLFSFYLPPNSGERVS 237          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Nematostella
Match: EDO32053 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW63])

HSP 1 Score: 186.422 bits (472), Expect = 1.106e-53
Identity = 90/247 (36.44%), Postives = 151/247 (61.13%), Query Frame = 2
Query:  260 ESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNAD---GNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMD-NITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMT 988
            E  L+ YLL++  Y+   RPV N+++ ++++L + + Q+I + E+ QI+  NVWIR  W D+ L+W P DFG +    I P RVW+PD++L+N  D   G+       NV+V  +G+  W+ PAI  S C I V +FPFD QSCE+ FGSWT D  ++ + ++  +  +D  ++  +  W++     R D  +      P   + YTIIL+R+++FY  N+++P  +I+LL++  FYLP ++GE+++
Sbjct:    1 EPALLKYLLQN--YSKDARPVLNVNDPVQLKLGIMLRQVIDLNERDQILTINVWIRQYWKDHLLKWNPDDFGGIKSINIAPDRVWKPDVLLYNRTDLRFGSLNNKIDTNVIVNHDGSTTWLAPAIIKSECKIDVRFFPFDVQSCELTFGSWTHDGLKIDI-HLKQESGIDTENFSDNREWFLKGTSGRRDVKMYICCPEPYPTVTYTIILRRRAMFYVFNMVLPTGVIALLSLFSFYLPPNSGERVS 244          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Nematostella
Match: EDO43642 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RY59])

HSP 1 Score: 180.259 bits (456), Expect = 8.810e-52
Identity = 92/248 (37.10%), Postives = 157/248 (63.31%), Query Frame = 2
Query:  260 ESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVS---WQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGAD--QSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMT 988
            E+KL+  L  +  YNP +RPV   ++++ V+L +++ QII ++E++QI+ SN+W+R KW +  L W+P+ FGN+    + P RVW+PD++L+NNAD + + +   +   V +  +G+ LW+ P IF S+C   V YFPFD+QSC +KFGSWT+D  +L +   +G   + +  +  +G W +    S   N+   D    P   + +T+ ++R +LFY +N++IPC LI+ LT+  + LP ++GE++T
Sbjct:    2 ENKLLKDLFSN--YNPSVRPVLKATSSVTVQLGVSLHQIIDLDERNQILISNLWVRQKWNNPLLTWDPTHFGNLTSINVDPLRVWRPDLILYNNADRDTDGALDRFYTKVQLKFDGSSLWLAPIIFKSTCKFDVAYFPFDEQSCILKFGSWTYDGLRLNIVPENG-TDIGIDKFVSNGEWELTGV-SANRNVMFYDCCVEPYPDVTFTLKIRRLTLFYYMNMLIPCFLITGLTLLSYILPPESGERIT 245          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Medaka
Match: chrna3 (neuronal acetylcholine receptor subunit alpha-3 [Source:NCBI gene;Acc:101159658])

HSP 1 Score: 299.671 bits (766), Expect = 3.865e-93
Identity = 209/555 (37.66%), Postives = 321/555 (57.84%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLSQXXXXXXXXXXXXXXLFANPWNNYQNMSRHSLDSESDKIPDS-KRTKCTCSASTNNI--DRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYI 1900
            GT  E++   + +    YN  IRPV N+S+ + V+  ++M Q++ V+E +QIM++N+W+R  W DY+L+W P +FG V+  R+  +R+W+PDIVL+NNA G+++V  +   L+  NG+V WIPPAIF SSC I V YFPFD Q+C MKFGSWT+D +++ L  I     ++L D+W+SG W ++D P    +I     +   T I Y++ ++R  LFYT+N+IIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W+R+ F+ +LP+ + M RPER       +P   +V +        +V  +   P   V+S   L+K +++ +  +    +  N   IL     +N  N S  S    S   P S +R  C     +  +  D  G               +     +  F   +     I    L +S  +R AI ++ +I+E++ ++ + K++  DWKYVA V DRI L +F +V I GT G+ L+ + +
Sbjct:   22 GTCSEAEHKLFSVIFSNYNQYIRPVENVSDPVIVQFEVSMSQLVKVDEVNQIMETNLWLRHIWNDYKLKWNPKEFGGVEFIRVPSNRIWKPDIVLYNNAVGDFQVDDKTKALLRFNGDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLIGST--INLKDFWESGEWTIIDAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPCWVRRVFLGLLPRVMFMTRPER-------DPEKVAVGS--------SVQTVHLRPCT-VHSSGTLQK-HKARAPGSSMMSSLTNRQRILNNTELSNLNNRSGDSNRCASSGFPGSGERCNCCWQKRSGKLPYDSEGGSGGLGCLGALSGGGAMGIGGETQFSSSESLDGRIM-SLLPLSPEIREAIESVKYIAENMRLQNDAKEVQDDWKYVAMVIDRIFLWVFVLVCILGTAGLFLQPLLL 556          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Medaka
Match: ENSORLT00000017941.2 (neuronal acetylcholine receptor subunit alpha-3-like [Source:NCBI gene;Acc:101168601])

HSP 1 Score: 255.758 bits (652), Expect = 3.024e-77
Identity = 153/332 (46.08%), Postives = 228/332 (68.67%), Query Frame = 2
Query:  251 TQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
            ++ E +L   L   ++YN  IRPV N+S+ + VE  +++ Q++ V+E +QIM++N+W+R  W DY+LRW P++F  ++  R+  +++W+PDIVL+NNA G++ V  +   L+  +G + W+PPAIF SSC + + YFPFD Q+C MKFGSWT+D +++ L  I  K  V+L D+W+SG W ++D P    +I     +     I Y+  ++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M GW+   F+ VLP+ ++M RP
Sbjct:   26 SKAEDRLFRKLF--RRYNQFIRPVENVSDPVTVEFEVSISQLVKVDEVNQIMETNLWLRHIWNDYKLRWTPAEFDGIEFIRVPSNKIWRPDIVLYNNAVGDFLVEDKTKALLKFDGTITWVPPAIFKSSCPMDITYFPFDYQNCSMKFGSWTYDKAKIDLVLIGSK--VNLKDFWESGEWEIIDAPGYKHDIKYNCCEEIYPDITYSFYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPMTHTMPGWVHLVFLKVLPRIMLMRRP 353          

HSP 2 Score: 55.8398 bits (133), Expect = 9.170e-8
Identity = 25/63 (39.68%), Postives = 40/63 (63.49%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAV 1894
            VS  ++ AI ++ +I+E++      K++  DWKYVA V DRI L +F  V + GTMG+ L+ +
Sbjct:  423 VSPEVKQAIESVKYIAENMRSRNKAKEVEDDWKYVAMVIDRIFLWVFVTVCLLGTMGLFLQPL 485          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Medaka
Match: chrna6 (neuronal acetylcholine receptor subunit alpha-3 [Source:NCBI gene;Acc:101162933])

HSP 1 Score: 254.988 bits (650), Expect = 6.985e-76
Identity = 149/316 (47.15%), Postives = 222/316 (70.25%), Query Frame = 2
Query:  299 YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNIT-GADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
            YN  IRPV N+S+ + VE  +++ Q++ V+E +QIM++N+W+R  W DY+L+W P+++  ++  R+  +R+W+PDIVL+NNA G++ V+ +   L+  +G + W+PPAIF SSC + + YFPFD Q+C MKFGSWT+D +++ L  I  K  V+L D+W+SG W ++D P    +I     +   T I Y+  ++R  LFYT+NLIIPC+LIS LTV VFYLP+D GEK+TL IS+LLSL VFLL+I+  +P TS  IPLI ++LLFT I    +I ITV ++N ++R+P TH M  W+R  F+ +LP+ ++M RP
Sbjct:   74 YNQFIRPVENVSDPVTVEFEVSISQLVKVDEVNQIMETNLWLRHVWNDYKLKWTPAEYDGIEFIRVPSNRIWRPDIVLYNNAVGDFLVADKTKALLKFDGTITWVPPAIFKSSCPMDITYFPFDYQNCSMKFGSWTYDKAKIDLVLIGSK--VNLKDFWESGEWEIIDAPGYKHDIKYNCCEEIYTDITYSFYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPMTHTMPDWVRSVFLGLLPRVMLMRRP 387          

HSP 2 Score: 53.9138 bits (128), Expect = 3.768e-7
Identity = 24/61 (39.34%), Postives = 38/61 (62.30%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLK 1888
            VS  ++ AI ++ +I+E+L      K++  DWKYVA V DRI L +F  V + GT+G+  +
Sbjct:  527 VSPEVKQAIESVKYIAENLRTRNKAKEVEDDWKYVAMVIDRIFLWVFVTVCVLGTVGLFFQ 587          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Medaka
Match: chrnb4 (neuronal acetylcholine receptor subunit beta-4-like [Source:NCBI gene;Acc:101159423])

HSP 1 Score: 251.906 bits (642), Expect = 2.626e-75
Identity = 168/392 (42.86%), Postives = 249/392 (63.52%), Query Frame = 2
Query:  227 ANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLS 1402
            A  Q+      E +L++ LL   +YNPLIRP  N +  + V+L +++ Q+I+V E+ QIM +NVWI   W DY+L W+P+ +  +   RI    VW PDIVL+NNADG YEV+   NV+V  NG+V W+PPAI+ S+C I V++FPFD Q+C +KF SWT+D +++ L  I   +   + D+  SG W ++  P R   +   D +    + Y  I++RK LFYT+NLIIPC+LI+ L + VFYLP+D GEKMTL IS+LL+L VFLLLIS+I+PPTS  +PLI K+L+FT ++  F+I  +V ++N + RSP+TH M  W++  F+  LP  L M+RP   +S   +   ++  R +++  +I  +DY   + + F  S S L  S  + S
Sbjct:   38 ALMQSCKSADSEERLMNMLLGKHRYNPLIRPAINRTERVEVKLQVSLAQLISVNEREQIMTTNVWITQHWVDYRLSWDPAKYEGIQKLRIPSRHVWLPDIVLYNNADGIYEVTVFTNVIVLFNGSVNWLPPAIYKSACKIEVKHFPFDLQNCTLKFRSWTYDHTEIDL--ILKSEAASMDDFTPSGEWDIIALPGR-RTVNPTDPT-YVDLTYDFIIKRKPLFYTINLIIPCILITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIITSVCVLNVHHRSPSTHTMPSWVKLIFLKKLPALLFMKRPN-NFSGCQR---LRQQRCLRARRSILGLDYPNASKACFTLSSSALLPSDSAFS 421          

HSP 2 Score: 62.003 bits (149), Expect = 1.089e-9
Identity = 22/60 (36.67%), Postives = 43/60 (71.67%), Query Frame = 2
Query: 1718 MRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVY 1897
            ++ A+  + F++EH+  +++++ ++ DWKYVA V DR+ L IF IV + GT+G+ L+ ++
Sbjct:  471 VKEAVKGVRFVAEHMMADDDDQSVIEDWKYVAMVVDRMFLWIFVIVCVVGTLGLFLQPLF 530          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Medaka
Match: chrna4b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:101158195])

HSP 1 Score: 252.677 bits (644), Expect = 1.606e-74
Identity = 163/343 (47.52%), Postives = 228/343 (66.47%), Query Frame = 2
Query:  221 SVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPE--TQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
             V + Q       E +L+  L    +YN L RPV N S+T+ V   L++ Q+I V+EK+Q+M +NVW++ +W DY+LRW P D+ NV   RI    +W+PDIVL+NNADG++ V+      +  +G + W+PPAI+ SSC+I V +FPFDQQSC+MKFGSWT+D +++ L  I     VD  DYW+SG W +V+   +  N    +   E    I Y  I++R  LFYT+NLIIPC+LIS LTV VFYLP+  GEK+TL IS+LLSL VFLLLI+ I+P TS  IPLI ++LLFT +    +I IT+ ++N + RSP TH M  W+R+ F+D++P+ L M+RP
Sbjct:   32 GVVSSQIEPRAHAEERLLQNLF--ARYNKLSRPVQNTSDTVLVHFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPEDYENVTSIRIPSEIIWRPDIVLYNNADGDFAVTHLTKAHLFYDGQIKWMPPAIYKSSCSIDVTFFPFDQQSCKMKFGSWTYDRAKIDL--ISMASDVDQMDYWESGEWVIVNAVGKY-NTKKYECCAEIYADITYYFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSQCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMVFVTLSIIITIFVLNVHHRSPQTHGMPHWVRRVFLDLVPRVLFMKRP 369          

HSP 2 Score: 56.225 bits (134), Expect = 8.133e-8
Identity = 25/60 (41.67%), Postives = 38/60 (63.33%), Query Frame = 2
Query: 1706 VSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILL 1885
            VS  M+ AI  + +I++HL   + +  +  DWKYVA V DRI L +F +V I G++G+ L
Sbjct:  579 VSPAMQRAIEGVQYIADHLRAGDADFSVKEDWKYVAMVIDRIFLWMFVLVCILGSVGLFL 638          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Planmine SMEST
Match: SMESG000029213.1 (SMESG000029213.1)

HSP 1 Score: 1063.52 bits (2749), Expect = 0.000e+0
Identity = 587/588 (99.83%), Postives = 587/588 (99.83%), Query Frame = 2
Query:  191 VIFILQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLSQXXXXXXXXXXXXXXLFANPWNNYQNMSRHSLDSESDKIPDSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSAASL 1954
            VIFILQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTLSISILLSLVVFLLLISRILPPTSSSIPLISKFLLFTFIMNIFTITITVMIINWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRT KSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLSQDNINSENTNNSDNILFANPWNNYQNMSRHSLDSESDKIPDSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSAASL
Sbjct:   15 VIFILQHLQNSVANYQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTLSISILLSLVVFLLLISRILPPTSSSIPLISKFLLFTFIMNIFTITITVMIINWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTAKSNNNIHNVDYIAYNPSAFVYSPSFLRKSYQSLSQDNINSENTNNSDNILFANPWNNYQNMSRHSLDSESDKIPDSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISANDAFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSAASL 602          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Planmine SMEST
Match: SMESG000031561.1 (SMESG000031561.1)

HSP 1 Score: 359.762 bits (922), Expect = 2.549e-117
Identity = 208/337 (61.72%), Postives = 269/337 (79.82%), Query Frame = 2
Query:  233 YQNRVGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
            Y  RV +++E KLI YL  HK YN LIRPVSN + T+ VEL L +IQII V+EK+QI+ SNVW+RL W D+QL+W PSDFGN++V R+   ++W+PDIVLFNNADGNYEVSW+PNVL+  +GN+LWIPP IF SSCTI V YFPFDQQ+C+M+FGSWTFDA+Q+ L +  G + VDLSD+WKS +W +++CP  + N T   Q+P T+IVY + ++RK+LFYTVNLI+PCVLI+ LT CVFYLP ++GEKM L IS+LL+LVVFLLL+S+ILPPTS SIPLISK+LLFTFI+NI  + +TVM IN ++R+P  H+M  WIR  F++  P  L+M+RP
Sbjct:   24 YTIRV-SEYEGKLIDYLFNHKGYNNLIRPVSNTTATLTVELGLGLIQIINVDEKNQIVISNVWLRLAWNDFQLQWNPSDFGNLNVIRVPSDQIWRPDIVLFNNADGNYEVSWKPNVLIYNDGNILWIPPTIFKSSCTIDVTYFPFDQQTCKMRFGSWTFDATQVALSWYSGIQFVDLSDFWKSETWDLLNCPGEIKNYTHPGQAPTTEIVYLLHIRRKTLFYTVNLILPCVLIAFLTFCVFYLPTESGEKMCLCISVLLALVVFLLLVSQILPPTSVSIPLISKYLLFTFILNIIAVIVTVMTINIHYRTPQIHQMPKWIRYCFMEKFPPLLLMKRP 359          

HSP 2 Score: 69.707 bits (169), Expect = 3.282e-12
Identity = 32/84 (38.10%), Postives = 56/84 (66.67%), Query Frame = 2
Query: 1694 KQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSA 1945
            ++++ +  +    +A+NFI+ +L+ E+   + + DWK+VA+V DR+ L++F IVT  GT GILL    I    +++EVIKK+S+
Sbjct:  402 REIEANENLDEIFAAVNFIAANLQSEDAIDQALHDWKFVASVIDRMMLILFIIVTTIGTFGILLNFETIDPNFNQDEVIKKFSS 485          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Planmine SMEST
Match: SMESG000031561.1 (SMESG000031561.1)

HSP 1 Score: 359.377 bits (921), Expect = 2.725e-117
Identity = 205/331 (61.93%), Postives = 266/331 (80.36%), Query Frame = 2
Query:  251 TQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERP 1243
            +++E KLI YL  HK YN LIRPVSN + T+ VEL L +IQII V+EK+QI+ SNVW+RL W D+QL+W PSDFGN++V R+   ++W+PDIVLFNNADGNYEVSW+PNVL+  +GN+LWIPP IF SSCTI V YFPFDQQ+C+M+FGSWTFDA+Q+ L +  G + VDLSD+WKS +W +++CP  + N T   Q+P T+IVY + ++RK+LFYTVNLI+PCVLI+ LT CVFYLP ++GEKM L IS+LL+LVVFLLL+S+ILPPTS SIPLISK+LLFTFI+NI  + +TVM IN ++R+P  H+M  WIR  F++  P  L+M+RP
Sbjct:   27 SEYEGKLIDYLFNHKGYNNLIRPVSNTTATLTVELGLGLIQIINVDEKNQIVISNVWLRLAWNDFQLQWNPSDFGNLNVIRVPSDQIWRPDIVLFNNADGNYEVSWKPNVLIYNDGNILWIPPTIFKSSCTIDVTYFPFDQQTCKMRFGSWTFDATQVALSWYSGIQFVDLSDFWKSETWDLLNCPGEIKNYTHPGQAPTTEIVYLLHIRRKTLFYTVNLILPCVLIAFLTFCVFYLPTESGEKMCLCISVLLALVVFLLLVSQILPPTSVSIPLISKYLLFTFILNIIAVIVTVMTINIHYRTPQIHQMPKWIRYCFMEKFPPLLLMKRP 357          

HSP 2 Score: 69.3218 bits (168), Expect = 3.856e-12
Identity = 32/84 (38.10%), Postives = 56/84 (66.67%), Query Frame = 2
Query: 1694 KQLKVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSA 1945
            ++++ +  +    +A+NFI+ +L+ E+   + + DWK+VA+V DR+ L++F IVT  GT GILL    I    +++EVIKK+S+
Sbjct:  400 REIEANENLDEIFAAVNFIAANLQSEDAIDQALHDWKFVASVIDRMMLILFIIVTTIGTFGILLNFETIDPNFNQDEVIKKFSS 483          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Planmine SMEST
Match: SMESG000064521.1 (SMESG000064521.1)

HSP 1 Score: 347.436 bits (890), Expect = 1.789e-111
Identity = 206/335 (61.49%), Postives = 264/335 (78.81%), Query Frame = 2
Query:  245 VGTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQ-IVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPE 1246
            + +  E +L  YL ++K YN LIRPV NL++ I V+  LA+IQ+I VEEK QI+KSNVWIR+KW DYQL WEP+DFG + V R++P  VW+PDIVLFNNADGNYEV WQPN+L+  +G +LWIPPAI+ SSC+I VEYFPFD+Q CEM+FGSWTF++ Q++L +  G   +DLS YW+SG+W ++ CP R+ N T      +TQ I+Y I ++RK+LFYTV LI+PC+LISLLTVCVFYLPAD   KMTL+ISILL+L+VF LLIS++LPPTS+SIPLISK+LLFTFI+NI  I ITV+IIN N+R+P TH M  W++K FI+ LP  L M RP+
Sbjct:   28 LASDDEGRLKDYLFKYKGYNQLIRPVVNLTDVIDVKFELALIQLINVEEKKQILKSNVWIRMKWVDYQLVWEPADFGGIKVTRVSPKIVWKPDIVLFNNADGNYEVLWQPNILIQYDGEILWIPPAIYESSCSILVEYFPFDEQVCEMRFGSWTFNSKQVSLSW-SGSSKIDLSTYWQSGTWDIISCPGRITNGTMDSSGIDTQEIIYQITIRRKTLFYTVILILPCLLISLLTVCVFYLPADDHHKMTLTISILLALIVFFLLISKLLPPTSTSIPLISKYLLFTFIVNIIVIVITVIIINLNYRTPRTHYMPSWVKKVFIEFLPIVLGMSRPK 361          

HSP 2 Score: 60.8474 bits (146), Expect = 2.220e-9
Identity = 27/71 (38.03%), Postives = 44/71 (61.97%), Query Frame = 2
Query: 1703 KVSNRMRSAISAINFISEHLEMEENNKKIMRDWKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVD 1915
            ++++ MR AI +INF+S H E +       +DW++VA+V DR+ L+IF+ VT+ GT  + L+  Y     D
Sbjct:  503 EINHEMRQAILSINFLSSHTESQGEFSMGKQDWQFVASVIDRLMLLIFSTVTLIGTAALFLRTPYFFSLFD 573          
BLAST of Acetylcholine receptor subunit beta-like protein vs. Planmine SMEST
Match: SMESG000020665.1 (SMESG000020665.1)

HSP 1 Score: 220.705 bits (561), Expect = 1.787e-64
Identity = 147/377 (38.99%), Postives = 237/377 (62.86%), Query Frame = 2
Query:  248 GTQHESKLIHYLLEHKQYNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQLRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPN--VLVTPNGNVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGKKH---------------VDLSDYWKSGSWYMVDCPSRMD-NITGADQSPETQIVYTIILQRKSLFYTVNLIIPCVLISLLTVCVFYLPADAGEKMTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXWNFRSPNTHKMSGWIRKWFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTVKSNNNIHN 1324
            GT+   +L   L+  + Y+P +RPV + ++T+ + + L+++QI+ V+EK+QI+  N+W+  +W D    W+P+D+GNV    I    +W PD+VL+NNA G+++++   N  V+V  +G+VLW PP IF S C I+VE+FPFD+Q C++K G+WT +   + L +   K                 +DLS Y  +  W +++  ++ +         P   I + + L+R++LFY +NLI PC+ IS LT+ VFYLPAD+GEK++LSISILLSL VF LLI  I PPTS  +PLI K+LLFT I+   ++  TV+++N ++RSP+THKM+ W+R  F   LPK L M++P++ Y  +  +   ++    ++++NI +
Sbjct:   16 GTKDARRLYDDLMRRRGYDPSVRPVKSYTSTVLLYMNLSLVQILDVDEKNQIIVLNMWVNQEWNDPNFVWDPNDYGNVTELNIPSREIWTPDLVLYNNAGGDWDLASSSNTKVIVNHDGSVLWKPPIIFKSQCGINVEFFPFDKQLCDLKIGTWTHNGFLIDLRHTAQKNQSQASFDNCNAKIDYAIDLSQYVNNVEWDLLEVSAKRNIEFYPCCTEPYLDISFNVSLRRRTLFYGINLICPCLAISFLTILVFYLPADSGEKVSLSISILLSLTVFFLLIFDISPPTSIVVPLILKYLLFTMILVTLSVMATVLVLNIHWRSPDTHKMAPWVRSVFTSTLPKLLFMKKPDQDYRDNETDDIPETFHEDETDSNIQD 392          
The following BLAST results are available for this feature:
BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CHRNB42.703e-8148.41cholinergic receptor nicotinic beta 4 subunit [Sou... [more]
CHRNB22.357e-7947.29cholinergic receptor nicotinic beta 2 subunit [Sou... [more]
CHRNB23.883e-7947.29cholinergic receptor nicotinic beta 2 subunit [Sou... [more]
CHRNB21.721e-7747.01cholinergic receptor nicotinic beta 2 subunit [Sou... [more]
CHRNA37.297e-7745.65cholinergic receptor nicotinic alpha 3 subunit [So... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
acr-32.136e-8350.57Acetylcholine receptor subunit beta-type acr-3 [S... [more]
acr-23.467e-7945.43Acetylcholine receptor subunit beta-type acr-2 [S... [more]
lev-13.687e-7948.15Acetylcholine receptor subunit beta-type lev-1 [S... [more]
lev-13.119e-7644.44Acetylcholine receptor subunit beta-type lev-1 [S... [more]
unc-295.173e-7649.53Acetylcholine receptor subunit beta-type unc-29 [... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nAChRbeta13.317e-10559.10gene:FBgn0000038 transcript:FBtr0073299[more]
nAChRalpha41.173e-7746.67gene:FBgn0266347 transcript:FBtr0332872[more]
nAChRalpha42.264e-7746.67gene:FBgn0266347 transcript:FBtr0300118[more]
nAChRalpha43.298e-7746.36gene:FBgn0266347 transcript:FBtr0346707[more]
nAChRalpha16.171e-7648.43gene:FBgn0000036 transcript:FBtr0334916[more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
chrna32.414e-8148.02cholinergic receptor, nicotinic, alpha 3 [Source:Z... [more]
chrna2a1.701e-7748.02cholinergic receptor, nicotinic, alpha 2a (neurona... [more]
chrna4a2.472e-7637.73cholinergic receptor, nicotinic, alpha 4a [Source:... [more]
chrnb5a1.293e-7545.35cholinergic receptor, nicotinic, beta 5a [Source:Z... [more]
chrnb5a2.038e-7546.59cholinergic receptor, nicotinic, beta 5a [Source:Z... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
chrnb23.270e-7945.51cholinergic receptor nicotinic beta 2 subunit [Sou... [more]
chrnb23.970e-7946.27cholinergic receptor nicotinic beta 2 subunit [Sou... [more]
usp163.152e-7847.75ubiquitin specific peptidase 16 [Source:Xenbase;Ac... [more]
usp169.510e-7848.02ubiquitin specific peptidase 16 [Source:Xenbase;Ac... [more]
usp162.198e-7747.45ubiquitin specific peptidase 16 [Source:Xenbase;Ac... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Chrnb28.847e-8046.43cholinergic receptor, nicotinic, beta polypeptide ... [more]
Chrna31.825e-7946.25cholinergic receptor, nicotinic, alpha polypeptide... [more]
Chrna34.412e-7946.25cholinergic receptor, nicotinic, alpha polypeptide... [more]
Chrna34.771e-7946.25cholinergic receptor, nicotinic, alpha polypeptide... [more]
Chrnb28.683e-7746.15cholinergic receptor, nicotinic, beta polypeptide ... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P04755|ACH3_DROME5.549e-10458.81Acetylcholine receptor subunit beta-like 1 OS=Dros... [more]
sp|Q93149|ACR3_CAEEL2.717e-8250.57Acetylcholine receptor subunit beta-type acr-3 OS=... [more]
sp|P30926|ACHB4_HUMAN1.298e-8048.41Neuronal acetylcholine receptor subunit beta-4 OS=... [more]
sp|P09484|ACHB2_CHICK6.935e-8046.20Neuronal acetylcholine receptor subunit beta-2 OS=... [more]
sp|P12390|ACHB2_RAT5.780e-7946.43Neuronal acetylcholine receptor subunit beta-2 OS=... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DUG91.154e-12864.79Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GCT62.025e-12866.77Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HFW92.737e-12865.98Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8H1571.186e-12766.77Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A564Z3102.154e-12743.30Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
chrna4b4.832e-8736.85neuronal acetylcholine receptor subunit alpha-2 [S... [more]
chrna34.698e-8247.46neuronal acetylcholine receptor subunit alpha-3-li... [more]
chrna4b5.536e-8134.51neuronal acetylcholine receptor subunit alpha-2 [S... [more]
chrna35.669e-8147.46neuronal acetylcholine receptor subunit alpha-3-li... [more]
chrna68.918e-7746.46neuronal acetylcholine receptor subunit alpha-3 [S... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
chrna34.289e-7746.37cholinergic receptor, nicotinic, alpha 3 [Source:Z... [more]
chrnb3b7.090e-6642.40cholinergic receptor, nicotinic, beta 3b (neuronal... [more]
chrna2a2.848e-6449.49cholinergic receptor, nicotinic, alpha 2a (neurona... [more]
chrnb1l1.117e-6037.82cholinergic receptor, nicotinic, beta 1 (muscle) l... [more]
ENSPMAT00000008550.12.512e-4136.75pep scaffold:Pmarinus_7.0:GL476601:34:38373:1 gene... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO387225.829e-5738.87Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO420549.959e-5738.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO320521.099e-5436.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO320531.106e-5336.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO436428.810e-5237.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
chrna33.865e-9337.66neuronal acetylcholine receptor subunit alpha-3 [S... [more]
ENSORLT00000017941.23.024e-7746.08neuronal acetylcholine receptor subunit alpha-3-li... [more]
chrna66.985e-7647.15neuronal acetylcholine receptor subunit alpha-3 [S... [more]
chrnb42.626e-7542.86neuronal acetylcholine receptor subunit beta-4-lik... [more]
chrna4b1.606e-7447.52neuronal acetylcholine receptor subunit alpha-2 [S... [more]
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BLAST of Acetylcholine receptor subunit beta-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029213.10.000e+099.83SMESG000029213.1[more]
SMESG000031561.12.549e-11761.72SMESG000031561.1[more]
SMESG000031561.12.725e-11761.93SMESG000031561.1[more]
SMESG000064521.11.789e-11161.49SMESG000064521.1[more]
SMESG000020665.11.787e-6438.99SMESG000020665.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017192 ID=SMED30017192|Name=Acetylcholine receptor subunit beta-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1960bp
CCGATCTCGAATAGCGTAATCAGAATTGCATATTGATTACCTTATCTACA
GTGGGAAAAATCCTTTGATTAAATTTTCGATGAGTGAAGATCTGTTTGTC
GACTCATAATTGATCACTGGAACATTCCTTGATTTTTACATTATTAGGAT
GATACTAAATTTATTACTGAATACTAGGCTTATTACAATAGTTATTTTTA
TATTGCAGCATCTTCAAAATTCAGTGGCCAATTATCAAAATAGAGTCGGC
ACGCAACATGAATCTAAACTGATCCATTACTTATTGGAGCACAAACAATA
TAATCCGTTGATCAGACCCGTTTCCAATCTTTCCAACACAATCAGAGTTG
AACTCGCTTTAGCTATGATTCAGATAATCGCAGTTGAAGAGAAGTCTCAA
ATAATGAAAAGCAATGTCTGGATAAGATTGAAATGGACAGATTATCAACT
GAGATGGGAGCCGTCCGATTTTGGGAATGTCGATGTTGCCAGGATCACCC
CAAGTAGAGTATGGCAGCCTGATATCGTTTTATTTAACAATGCAGATGGG
AATTATGAGGTTTCTTGGCAGCCAAATGTATTAGTTACTCCAAATGGTAA
TGTGCTTTGGATACCTCCAGCAATTTTTATAAGCTCATGCACAATACATG
TGGAATATTTTCCTTTTGATCAACAAAGTTGTGAAATGAAATTTGGATCG
TGGACATTTGATGCTTCTCAGTTAACTCTTGGTTACATAGACGGGAAAAA
GCATGTAGACCTAAGTGACTATTGGAAGAGTGGAAGCTGGTATATGGTCG
ACTGTCCTTCTCGTATGGATAATATAACTGGAGCCGATCAATCTCCAGAA
ACTCAAATTGTATATACAATTATACTACAAAGAAAAAGTCTTTTTTATAC
AGTTAATTTAATTATTCCCTGTGTTCTAATTTCTTTGCTTACAGTCTGTG
TTTTCTATTTACCAGCTGATGCTGGCGAAAAGATGACACTTTCTATTTCA
ATTCTGCTATCATTAGTTGTCTTTTTACTTTTGATCAGTAGAATTCTTCC
ACCGACATCATCTTCCATTCCTCTAATTAGCAAGTTTTTACTATTTACAT
TTATTATGAATATCTTTACTATAACTATTACTGTGATGATAATAAATTGG
AATTTTCGGTCTCCAAACACTCATAAAATGTCGGGATGGATCAGAAAATG
GTTCATTGATGTGCTTCCAAAGTTTTTGATGATGGAAAGACCAGAAAGAA
AATACTCAATGTCTAATAAAGAACCTAACGTCAAGTCAGTCAGAACTGTT
AAAAGCAATAACAACATTCATAATGTCGACTACATAGCTTATAATCCTTC
TGCATTTGTTTATTCTCCATCGTTTCTTAGAAAGTCCTATCAGTCATTGT
CTCAAGATAACATTAACTCTGAAAATACGAACAATTCTGATAATATCCTT
TTTGCAAATCCCTGGAATAATTATCAAAACATGTCTCGTCATTCTTTAGA
CAGTGAAAGTGACAAAATTCCTGATAGTAAAAGAACAAAATGTACATGTT
CGGCCTCGACAAATAACATTGATCGGAGTGGTACTGATAAAACCCATTGT
GATACATTTGAATGCCCATCATCCCCATCAGATATATCAGCAAATGATGC
ATTTTTCGATCAAAGAAAAGATTTTCGGAGGAATATTTATCCAAAACAAC
TAAAAGTCTCAAATAGAATGCGCTCGGCGATTTCTGCAATAAATTTTATT
TCAGAACATTTGGAAATGGAAGAAAATAATAAAAAGATAATGCGAGATTG
GAAATATGTGGCTGCAGTATTCGACAGGATTCAGTTAGTTATATTCACCA
TTGTGACTATAGCTGGGACAATGGGAATTTTGCTCAAAGCAGTTTATATT
TTAGAATATGTAGATGAGAACGAAGTCATTAAAAAGTACTCGGCTGCTTC
CTTGTAAAAC
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protein sequence of SMED30017192-orf-1

>SMED30017192-orf-1 ID=SMED30017192-orf-1|Name=SMED30017192-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=603bp
MILNLLLNTRLITIVIFILQHLQNSVANYQNRVGTQHESKLIHYLLEHKQ
YNPLIRPVSNLSNTIRVELALAMIQIIAVEEKSQIMKSNVWIRLKWTDYQ
LRWEPSDFGNVDVARITPSRVWQPDIVLFNNADGNYEVSWQPNVLVTPNG
NVLWIPPAIFISSCTIHVEYFPFDQQSCEMKFGSWTFDASQLTLGYIDGK
KHVDLSDYWKSGSWYMVDCPSRMDNITGADQSPETQIVYTIILQRKSLFY
TVNLIIPCVLISLLTVCVFYLPADAGEKMTLSISILLSLVVFLLLISRIL
PPTSSSIPLISKFLLFTFIMNIFTITITVMIINWNFRSPNTHKMSGWIRK
WFIDVLPKFLMMERPERKYSMSNKEPNVKSVRTVKSNNNIHNVDYIAYNP
SAFVYSPSFLRKSYQSLSQDNINSENTNNSDNILFANPWNNYQNMSRHSL
DSESDKIPDSKRTKCTCSASTNNIDRSGTDKTHCDTFECPSSPSDISAND
AFFDQRKDFRRNIYPKQLKVSNRMRSAISAINFISEHLEMEENNKKIMRD
WKYVAAVFDRIQLVIFTIVTIAGTMGILLKAVYILEYVDENEVIKKYSAA
SL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004888transmembrane signaling receptor activity
GO:0004889acetylcholine-activated cation-selective channel activity
GO:0005216ion channel activity
GO:0005230extracellular ligand-gated ion channel activity
GO:0005515protein binding
GO:0008144drug binding
GO:0015464acetylcholine receptor activity
GO:0022848acetylcholine-gated cation-selective channel activity
GO:0042166acetylcholine binding
GO:0046982protein heterodimerization activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000418head
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036734Neur_chan_lig-bd_sf
IPR036719Neuro-gated_channel_TM_sf
IPR006201Neur_channel
IPR002394Nicotinic_acetylcholine_rcpt
IPR027361Acetylcholine_rcpt_TM
IPR006202Neur_chan_lig-bd
IPR006029Neurotrans-gated_channel_TM
IPR018000Neurotransmitter_ion_chnl_CS
Vocabulary: biological process
TermDefinition
GO:0006811ion transport
GO:0006810transport
GO:0001508action potential
GO:0001661conditioned taste aversion
GO:0001666response to hypoxia
GO:0006816calcium ion transport
GO:0006939smooth muscle contraction
GO:0007165signal transduction
GO:0007271synaptic transmission, cholinergic
GO:0007274neuromuscular synaptic transmission
GO:0007601visual perception
GO:0007605sensory perception of sound
GO:0007612learning
GO:0007613memory
GO:0007626locomotory behavior
GO:0008306associative learning
GO:0008542visual learning
GO:0014059regulation of dopamine secretion
GO:0019233sensory perception of pain
GO:0021562vestibulocochlear nerve development
GO:0021631optic nerve morphogenesis
GO:0021771lateral geniculate nucleus development
GO:0021952central nervous system projection neuron axonogenesis
GO:0021955central nervous system neuron axonogenesis
GO:0030890positive regulation of B cell proliferation
GO:0032225regulation of synaptic transmission, dopaminergic
GO:0032226positive regulation of synaptic transmission, dopaminergic
GO:0033603positive regulation of dopamine secretion
GO:0034220ion transmembrane transport
GO:0035094response to nicotine
GO:0035095behavioral response to nicotine
GO:0035176social behavior
GO:0042053regulation of dopamine metabolic process
GO:0042113B cell activation
GO:0042220response to cocaine
GO:0042320regulation of circadian sleep/wake cycle, REM sleep
GO:0042391regulation of membrane potential
GO:0045188regulation of circadian sleep/wake cycle, non-REM sleep
GO:0045471response to ethanol
GO:0045759negative regulation of action potential
GO:0048814regulation of dendrite morphogenesis
GO:0050877neurological system process
GO:0050890cognition
GO:0051291protein heterooligomerization
GO:0051899membrane depolarization
GO:0051963regulation of synapse assembly
GO:0060078regulation of postsynaptic membrane potential
GO:0060079excitatory postsynaptic potential
GO:0060084synaptic transmission involved in micturition
GO:0095500acetylcholine receptor signaling pathway
GO:1905144response to acetylcholine
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0045211postsynaptic membrane
GO:0016020membrane
GO:0005886plasma membrane
GO:0005892acetylcholine-gated channel complex
GO:0009897external side of plasma membrane
GO:0030054cell junction
GO:0044853plasma membrane raft
GO:0045202synapse
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002394Nicotinic acetylcholine receptorPRINTSPR00254NICOTINICRcoord: 74..90
score: 42.09
coord: 144..162
score: 49.77
coord: 108..122
score: 27.78
coord: 126..138
score: 63.08
IPR006201Neurotransmitter-gated ion-channelPRINTSPR00252NRIONCHANNELcoord: 120..131
score: 47.02
coord: 164..178
score: 50.65
coord: 87..103
score: 35.72
coord: 241..253
score: 36.69
IPR006201Neurotransmitter-gated ion-channelPANTHERPTHR18945NEUROTRANSMITTER GATED ION CHANNELcoord: 43..586
IPR006029Neurotransmitter-gated ion-channel transmembrane domainPFAMPF02932Neur_chan_membcoord: 254..578
e-value: 5.9E-68
score: 229.5
IPR036734Neurotransmitter-gated ion-channel ligand-binding domain superfamilyGENE3DG3DSA:2.70.170.10coord: 36..246
e-value: 1.2E-69
score: 235.8
IPR036734Neurotransmitter-gated ion-channel ligand-binding domain superfamilySUPERFAMILYSSF63712Nicotinic receptor ligand binding domain-likecoord: 37..246
IPR006202Neurotransmitter-gated ion-channel ligand-binding domainPFAMPF02931Neur_chan_LBDcoord: 39..246
e-value: 4.6E-63
score: 212.5
NoneNo IPR availableGENE3DG3DSA:1.20.58.390coord: 248..365
e-value: 3.2E-41
score: 142.2
NoneNo IPR availableGENE3DG3DSA:1.20.58.390coord: 514..591
e-value: 1.8E-13
score: 52.5
NoneNo IPR availablePANTHERPTHR18945:SF784ACETYLCHOLINE RECEPTOR SUBUNIT BETA-LIKE 1coord: 43..586
NoneNo IPR availableCDDcd19032LGIC_ECD_nAChR_proto_beta-likecoord: 38..246
e-value: 1.94332E-113
score: 335.448
NoneNo IPR availableCDDcd19064LGIC_TM_nAChRcoord: 248..337
e-value: 1.91834E-40
score: 140.726
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..27
score: 0.703
NoneNo IPR availableTMHMMTMhelixcoord: 314..336
NoneNo IPR availableTMHMMTMhelixcoord: 280..299
NoneNo IPR availableTMHMMTMhelixcoord: 249..271
NoneNo IPR availableTMHMMTMhelixcoord: 563..585
IPR018000Neurotransmitter-gated ion-channel, conserved sitePROSITEPS00236NEUROTR_ION_CHANNELcoord: 164..178
IPR036719Neurotransmitter-gated ion-channel transmembrane domain superfamilySUPERFAMILYSSF90112Neurotransmitter-gated ion-channel transmembrane porecoord: 248..584