DNA topoisomerase 2-binding protein 1

Overview
NameDNA topoisomerase 2-binding protein 1
Smed IDSMED30017131
Length (bp)3003
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA topoisomerase 2-binding protein 1 (SMED30017131) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA topoisomerase 2-binding protein 1 (SMED30017131) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA topoisomerase 2-binding protein 1 (SMED30017131) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA topoisomerase 2-binding protein 1 (SMED30017131) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017131

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30017131h1SMcG0014842 SmedASXL_014705SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30017131h1SMcG0014842 SmedASXL_014705SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30017131h1SMcG0014842 dd_Smed_v4_14737_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30017131h1SMcG0014842 dd_Smed_v6_14737_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30017131h1SMcG0014842 dd_Smed_v4_14737_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30017131h1SMcG0014842 SMED_26858_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30017131h1SMcG0014842 SMED_26858_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30017131h1SMcG0014842 SMED_16452_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30017131h1SMcG0014842 SMED_16452_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Human
Match: TOPBP1 (DNA topoisomerase II binding protein 1 [Source:HGNC Symbol;Acc:HGNC:17008])

HSP 1 Score: 141.739 bits (356), Expect = 2.380e-33
Identity = 121/421 (28.74%), Postives = 188/421 (44.66%), Query Frame = 3 
Query:  195 LSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRA---YIPGLSQTDSS----------SNNSAGQNDKIICDNKLNTK---------------------IWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGD-ASKLKE---KEDDITYVNG-DWLQACLDGKALSDSKQFAIEIPKSPIAVPISN 1340
            L  I  E+ L+    N    YI   F      +LK       GPQ ++  + +   +P  E P+++  + +V +  T   +E ++E+   V MM G V +DLN S   LI+  +G++KY  A+ +  P++   WI  +WE +Q        D   +E ++ PIF    I +TG   L+R+ ++++ +K GG Y   L +  CTHLI  +  G+K+ECAK+W +  V  QW  DS+ KG+  DE  Y+   R     +P  S   S           SN +A    + IC N LN+K                     +   C I         ++K   LI   GG     LN+     VTH+IVGD   +LK+   K     +V G  WL  C   K    S++  I     P+ +P+S+
Sbjct:   39 LQIITEEEALKIKE-NDRSLYICDPFSGVVFDHLKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTD-INMEDFKCPIFLGCIICVTGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDESIYKTEPRPEAKTMPNSSTPTSQINTIDNVSNISNINASCVSESIC-NSLNSKLEPTLENLENLDVSAFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNE----DVTHVIVGDYDDELKQFWNKSAHRPHVVGAKWLLECF-SKGYMLSEEPYIHANYQPVEIPVSH 451

HSP 2 Score: 89.7373 bits (221), Expect = 2.155e-17
Identity = 51/164 (31.10%), Postives = 85/164 (51.83%), Query Frame = 2 
Query: 2150 ENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNG-----GGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVP 2626
            E K + IF L +++    +     ++KLGG +     ++   TH+V G P R EKYLA++AAGKWVLH SY++    AG +++E  Y+WG   +S    L  +  +  +  LA     K+           G+F  W+V L  ++ +     R+++SGG  ++P
Sbjct: 1258 ELKKQYIFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGS--SSILDVLTGINVQQRRLALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLP 1419
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Human
Match: TOPBP1 (DNA topoisomerase II binding protein 1 [Source:HGNC Symbol;Acc:HGNC:17008])

HSP 1 Score: 140.198 bits (352), Expect = 6.278e-33
Identity = 121/426 (28.40%), Postives = 189/426 (44.37%), Query Frame = 3 
Query:  195 LSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRA---YIPG---------------LSQTDSSSNNSAGQNDKIICDNKLNTK---------------------IWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGD-ASKLKE---KEDDITYVNG-DWLQACLDGKALSDSKQFAIEIPKSPIAVPISN 1340
            L  I  E+ L+    N    YI   F      +LK       GPQ ++  + +   +P  E P+++  + +V +  T   +E ++E+   V MM G V +DLN S   LI+  +G++KY  A+ +  P++   WI  +WE +Q        D   +E ++ PIF    I +TG   L+R+ ++++ +K GG Y   L +  CTHLI  +  G+K+ECAK+W +  V  QW  DS+ KG+  DE  Y+   R     +P                LS   + SN +A    + IC N LN+K                     +   C I         ++K   LI   GG     LN+     VTH+IVGD   +LK+   K     +V G  WL  C   K    S++  I     P+ +P+S+
Sbjct:   39 LQIITEEEALKIKE-NDRSLYICDPFSGVVFDHLKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTD-INMEDFKCPIFLGCIICVTGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDESIYKTEPRPEAKTMPNSSTPTSQINTIDSRTLSDVSNISNINASCVSESIC-NSLNSKLEPTLENLENLDVSAFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNE----DVTHVIVGDYDDELKQFWNKSAHRPHVVGAKWLLECF-SKGYMLSEEPYIHANYQPVEIPVSH 456

HSP 2 Score: 89.7373 bits (221), Expect = 2.175e-17
Identity = 51/164 (31.10%), Postives = 85/164 (51.83%), Query Frame = 2 
Query: 2150 ENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNG-----GGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVP 2626
            E K + IF L +++    +     ++KLGG +     ++   TH+V G P R EKYLA++AAGKWVLH SY++    AG +++E  Y+WG   +S    L  +  +  +  LA     K+           G+F  W+V L  ++ +     R+++SGG  ++P
Sbjct: 1263 ELKKQYIFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGS--SSILDVLTGINVQQRRLALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLP 1424
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Human
Match: ECT2 (epithelial cell transforming 2 [Source:HGNC Symbol;Acc:HGNC:3155])

HSP 1 Score: 94.3597 bits (233), Expect = 6.566e-19
Identity = 69/235 (29.36%), Postives = 112/235 (47.66%), Query Frame = 3 
Query:  321 GPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSS-SNNSAGQNDK 1013
            GP  +L+  +  E LP    PL+ T++ N+ +CFTGF +  K+EL   V ++H   G + KD N+    L++      K+R A  +G P++  +WI K WE     D     D++  E +++P F +  +S  GF   E+  +E++    GG Y P L    CTHL+  +   +        K+  V  +W   S++      E  Y L  +A  P L ++ S  S N+   N K
Sbjct:  118 GPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRK--KEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNE-FKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLP-LGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMY-LYEKANTPELKKSVSMLSLNTPNSNRK 347
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Human
Match: ECT2 (epithelial cell transforming 2 [Source:HGNC Symbol;Acc:HGNC:3155])

HSP 1 Score: 93.9745 bits (232), Expect = 6.859e-19
Identity = 69/235 (29.36%), Postives = 112/235 (47.66%), Query Frame = 3 
Query:  321 GPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSS-SNNSAGQNDK 1013
            GP  +L+  +  E LP    PL+ T++ N+ +CFTGF +  K+EL   V ++H   G + KD N+    L++      K+R A  +G P++  +WI K WE     D     D++  E +++P F +  +S  GF   E+  +E++    GG Y P L    CTHL+  +   +        K+  V  +W   S++      E  Y L  +A  P L ++ S  S N+   N K
Sbjct:  119 GPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRK--KEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNE-FKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLP-LGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMY-LYEKANTPELKKSVSMLSLNTPNSNRK 348
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Human
Match: ECT2 (epithelial cell transforming 2 [Source:HGNC Symbol;Acc:HGNC:3155])

HSP 1 Score: 93.9745 bits (232), Expect = 6.859e-19
Identity = 69/235 (29.36%), Postives = 112/235 (47.66%), Query Frame = 3 
Query:  321 GPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSS-SNNSAGQNDK 1013
            GP  +L+  +  E LP    PL+ T++ N+ +CFTGF +  K+EL   V ++H   G + KD N+    L++      K+R A  +G P++  +WI K WE     D     D++  E +++P F +  +S  GF   E+  +E++    GG Y P L    CTHL+  +   +        K+  V  +W   S++      E  Y L  +A  P L ++ S  S N+   N K
Sbjct:  119 GPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRK--KEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNE-FKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLP-LGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMY-LYEKANTPELKKSVSMLSLNTPNSNRK 348
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Celegans
Match: mus-101 (pep chromosome:WBcel235:I:10479283:10484969:-1 gene:WBGene00009507.1 transcript:F37D6.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mus-101)

HSP 1 Score: 94.7449 bits (234), Expect = 1.894e-19
Identity = 70/284 (24.65%), Postives = 135/284 (47.54%), Query Frame = 3 
Query:  117 VYFVEDTKTPLLVQAYNALDGMENANL-----SWIKT---EDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDL-NASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNT-DPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIIT-VNYQWLLDSVRKGYACDEKKYQ 935
            +YFV+    P + +  N  +  + A        WI +   ED+ ++  F     ++   F  K    L++ K+  +GP  +L+ + + + LP    P++S+  ++V++ FTG N   K+EL +K+  M G V   L + +  ++   +  T KY+AA    I ++   WI+ +WE +Q T           +  Y + +F  L++++T     +R    ++I   GG     ++   C++LI+ + +G K+  A +WK +  V  +W+   V  G+  D  KY 
Sbjct:   36 LYFVDLPNEPTVKEIRNLEELFKTAKAVGIMPEWIDSDALEDLQKSEDF-----FVLPCFRGKLFRKLQARKLKVYGPPIVLESIEDGKQLPQWNHPVYSSVFQDVKISFTGLNLTKKQELYEKIGWMCGVVGDALYHETTHLVTEKAEQTEKYKAAVNNSIKLMRIGWIDDLWETSQTTMGRFSALSRDSVNSYALRVFEGLEMAITSIDGADRTNFIQLIEDHGGKVPGTMSKTRCSYLISDKITGVKYAKAVEWKSMQIVQSRWIRKCVDLGHLVDAGKYH 314

HSP 2 Score: 71.2478 bits (173), Expect = 3.200e-12
Identity = 58/230 (25.22%), Postives = 100/230 (43.48%), Query Frame = 2 
Query: 2168 IFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLR----TSEFIELESVACRFTKFDLA---------LADAAKRF----HDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTI--------VPLIEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLIPFYLSARNDCERKKF 2782
            +  +  +D      L+E + KLGG +E   ++N + THL++    R  K L +IAAGKW L   Y+  S   GRW++E  ++W   +    + +   L+    R    +LA         +A+    F     +   G+F DWR  +  + ++    S I+E+GG  +        V  ++P   L    F  N    + L    +   Y  D++  +L  R++  R KF
Sbjct:  948 LLSVSNMDPQRAADLQETIMKLGGTIE--REFNKDVTHLIASNMQRAPKVLCSIAAGKWCLTPDYVTKSAEVGRWLDEKSFEWTREKLLACSKKNESLKERDSRKVSENLAAVCGLWRQRVAEMPVTFSVSMENRQNGAFSDWRCVIHLDDKRAGVFSSILEAGGAIVHSISEYVEVSTLKPNIVLASKEFPWNPQ-SAVLLKRDNIPIYVFDILYDFLIDRDNLNRTKF 1174
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Celegans
Match: ect-2 (ECT2 (Mammalian Rho GEF) homolog [Source:UniProtKB/TrEMBL;Acc:Q9U364])

HSP 1 Score: 59.3066 bits (142), Expect = 1.283e-8
Identity = 57/257 (22.18%), Postives = 118/257 (45.91%), Query Frame = 3 
Query:  345 LRNDETLPI-CEIPLFSTALKNVEVCFTGFNEEV--KKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQL-SGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQ---FLDCEPEFV 1094
            L+N++ + +    PL+   +K+V +       EV  K+EL D V  M G+V KD  +   + I+  +   K ++ S +G+P +  DW+ + W+  ++    D  +  +++++R+ +F  L +   GF   E + + + +   GG   P  T  + TH++    + E    A     + V  +W   S+ +G    E  + L +G+ +   L     SS+ S+     +   N  +    +DC ++    L+  P+++
Sbjct:  100 LKNEDLMTLRAARPLYCELMKDVTMKLAA---EVPNKRELVDLVHYMGGSVRKDTVSRTNVFIAAKVEA-KVQSISLVGVPTMRADWVTECWK-HRDDSYFDVMEPCFVDKHRLGVFEGLSLFFHGFKQTEIDDMLRSLENTGGKLAPSPT--LATHVVYNPDNDEVETLAVSSNQVHVTKEWFWVSLHRGCCAIEDNFALPTGKLHRKPL----ESSSVSSPIGKSLRSQNSRSLSSMRDCSVEGPTSLNMTPDYI 345
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Celegans
Match: ect-2 (ECT2 (Mammalian Rho GEF) homolog [Source:UniProtKB/TrEMBL;Acc:Q9U364])

HSP 1 Score: 59.3066 bits (142), Expect = 1.283e-8
Identity = 57/257 (22.18%), Postives = 118/257 (45.91%), Query Frame = 3 
Query:  345 LRNDETLPI-CEIPLFSTALKNVEVCFTGFNEEV--KKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQL-SGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQ---FLDCEPEFV 1094
            L+N++ + +    PL+   +K+V +       EV  K+EL D V  M G+V KD  +   + I+  +   K ++ S +G+P +  DW+ + W+  ++    D  +  +++++R+ +F  L +   GF   E + + + +   GG   P  T  + TH++    + E    A     + V  +W   S+ +G    E  + L +G+ +   L     SS+ S+     +   N  +    +DC ++    L+  P+++
Sbjct:  100 LKNEDLMTLRAARPLYCELMKDVTMKLAA---EVPNKRELVDLVHYMGGSVRKDTVSRTNVFIAAKVEA-KVQSISLVGVPTMRADWVTECWK-HRDDSYFDVMEPCFVDKHRLGVFEGLSLFFHGFKQTEIDDMLRSLENTGGKLAPSPT--LATHVVYNPDNDEVETLAVSSNQVHVTKEWFWVSLHRGCCAIEDNFALPTGKLHRKPL----ESSSVSSPIGKSLRSQNSRSLSSMRDCSVEGPTSLNMTPDYI 345
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Celegans
Match: ect-2 (ECT2 (Mammalian Rho GEF) homolog [Source:UniProtKB/TrEMBL;Acc:Q9U364])

HSP 1 Score: 59.3066 bits (142), Expect = 1.474e-8
Identity = 57/257 (22.18%), Postives = 118/257 (45.91%), Query Frame = 3 
Query:  345 LRNDETLPI-CEIPLFSTALKNVEVCFTGFNEEV--KKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQL-SGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQ---FLDCEPEFV 1094
            L+N++ + +    PL+   +K+V +       EV  K+EL D V  M G+V KD  +   + I+  +   K ++ S +G+P +  DW+ + W+  ++    D  +  +++++R+ +F  L +   GF   E + + + +   GG   P  T  + TH++    + E    A     + V  +W   S+ +G    E  + L +G+ +   L     SS+ S+     +   N  +    +DC ++    L+  P+++
Sbjct:  100 LKNEDLMTLRAARPLYCELMKDVTMKLAA---EVPNKRELVDLVHYMGGSVRKDTVSRTNVFIAAKVEA-KVQSISLVGVPTMRADWVTECWK-HRDDSYFDVMEPCFVDKHRLGVFEGLSLFFHGFKQTEIDDMLRSLENTGGKLAPSPT--LATHVVYNPDNDEVETLAVSSNQVHVTKEWFWVSLHRGCCAIEDNFALPTGKLHRKPL----ESSSVSSPIGKSLRSQNSRSLSSMRDCSVEGPTSLNMTPDYI 345
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Celegans
Match: ect-2 (ECT2 (Mammalian Rho GEF) homolog [Source:UniProtKB/TrEMBL;Acc:Q9U364])

HSP 1 Score: 59.3066 bits (142), Expect = 1.474e-8
Identity = 57/257 (22.18%), Postives = 118/257 (45.91%), Query Frame = 3 
Query:  345 LRNDETLPI-CEIPLFSTALKNVEVCFTGFNEEV--KKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQL-SGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQ---FLDCEPEFV 1094
            L+N++ + +    PL+   +K+V +       EV  K+EL D V  M G+V KD  +   + I+  +   K ++ S +G+P +  DW+ + W+  ++    D  +  +++++R+ +F  L +   GF   E + + + +   GG   P  T  + TH++    + E    A     + V  +W   S+ +G    E  + L +G+ +   L     SS+ S+     +   N  +    +DC ++    L+  P+++
Sbjct:  100 LKNEDLMTLRAARPLYCELMKDVTMKLAA---EVPNKRELVDLVHYMGGSVRKDTVSRTNVFIAAKVEA-KVQSISLVGVPTMRADWVTECWK-HRDDSYFDVMEPCFVDKHRLGVFEGLSLFFHGFKQTEIDDMLRSLENTGGKLAPSPT--LATHVVYNPDNDEVETLAVSSNQVHVTKEWFWVSLHRGCCAIEDNFALPTGKLHRKPL----ESSSVSSPIGKSLRSQNSRSLSSMRDCSVEGPTSLNMTPDYI 345
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Fly
Match: mus101 (gene:FBgn0002878 transcript:FBtr0073817)

HSP 1 Score: 107.071 bits (266), Expect = 4.164e-23
Identity = 64/244 (26.23%), Postives = 123/244 (50.41%), Query Frame = 3 
Query:  219 ILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDG--ADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQAS--GEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQL 938
            ++      K   ++   FE +    L+  + L  GP CL+  LR +E +P     ++STA+++++V  TG   + K+ELS  +  M G   +        LIS +I + KY  A+  G+PV+  DW+  +W+ ++ +   +G  A +   ++YR+PIF    I+ +G  +  ++ + +++   GG Y+      +   +I  Q     EK++ A ++K   +  +W+ DS  +GYA   K Y++
Sbjct:   62 LIAAGNLTKKDVFVLTQFEGEFFEQLQQTRALILGPPCLITCLRRNEPIPEGSSAIYSTAMRDLQVSATGITPQKKEELSRLINWMGGIYFQSFGHRTTHLISNTIKSSKYEQATLNGVPVMHVDWVQYVWDQSRRSQ-REGIMATDPDFDKYRLPIFFGANITCSGLDVARKDQVMRLVNDNGGIYHRAFRSQVVDIVITEQTKTDTEKYKAAIRYKKDVLLPEWIFDSCNRGYALPTKDYEV 304

HSP 2 Score: 80.8777 bits (198), Expect = 5.501e-15
Identity = 54/177 (30.51%), Postives = 83/177 (46.89%), Query Frame = 2 
Query: 2114 SKRKICEGAELIENKSEKIF-VLCTIDKASYVTLREAVKKLGGAM-ELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEP 2638
            +KR+   G+  ++      F + C  D      L   + +LGG + E   +Y+   THL+  RP R EK LA IAAGKW+L+  YI+ S A G +++E  Y+WG  +    I L ++A        A+          GGG+F D RV L         +  ++ +GG  I+    P
Sbjct: 1191 AKRRSSGGSPKMQYAGIPCFSISCGDDDEKRAELIARITQLGGKVCENLVNYDDSCTHLLCERPNRGEKMLACIAAGKWILNIQYIEQSHARGDFLDETLYEWGNPKA---INLPTLAPEEEPIAAAVHRWRTELSACGGGAFSDHRVILSMNERSGAPIRNVLRAGGACILEPTTP 1364
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Fly
Match: pbl (gene:FBgn0003041 transcript:FBtr0112817)

HSP 1 Score: 73.1738 bits (178), Expect = 8.924e-13
Identity = 57/223 (25.56%), Postives = 97/223 (43.50%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYA 914
            Y+   FE  S   +   K    GP  L       +TL     P+++ A++ V  CFTG  +  K EL+  V ++H   G + KDLN     LI    G  KY+ A    + VV   W+   W   +N+   D   E + + +R+  F   +I   GFP+ E + +  ++++ GG    +L    C+H++  + +       K      +   W   +++ GYA
Sbjct:  113 YVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRK--KDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWA-DRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVC-AELDDPECSHVVVDEHTTLTKPEPKNNHTHILKSDWFWYTIQNGYA 331
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Fly
Match: pbl (gene:FBgn0003041 transcript:FBtr0076771)

HSP 1 Score: 73.1738 bits (178), Expect = 9.288e-13
Identity = 57/223 (25.56%), Postives = 97/223 (43.50%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYA 914
            Y+   FE  S   +   K    GP  L       +TL     P+++ A++ V  CFTG  +  K EL+  V ++H   G + KDLN     LI    G  KY+ A    + VV   W+   W   +N+   D   E + + +R+  F   +I   GFP+ E + +  ++++ GG    +L    C+H++  + +       K      +   W   +++ GYA
Sbjct:   66 YVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRK--KDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWA-DRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVC-AELDDPECSHVVVDEHTTLTKPEPKNNHTHILKSDWFWYTIQNGYA 284
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Fly
Match: pbl (gene:FBgn0003041 transcript:FBtr0076772)

HSP 1 Score: 70.4774 bits (171), Expect = 6.858e-12
Identity = 55/198 (27.78%), Postives = 90/198 (45.45%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKF 839
            Y+   FE  S   +   K    GP  L       +TL     P+++ A++ V  CFTG  +  K EL+  V ++H   G + KDLN     LI    G  KY+ A    + VV   W+   W   +N+   D   E + + +R+  F   +I   GFP+ E + +  ++++ GG    +L    C+H++ M  +G  F
Sbjct:   66 YVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRK--KDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAW-ADRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVC-AELDDPECSHVV-MPNTGAVF 258
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Fly
Match: pbl (gene:FBgn0003041 transcript:FBtr0076773)

HSP 1 Score: 68.5514 bits (166), Expect = 2.269e-11
Identity = 53/204 (25.98%), Postives = 91/204 (44.61%), Query Frame = 3 
Query:  312 LAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYA 914
            L  GP  L       +TL     P+++ A++ V  CFTG  +  K EL+  V ++H   G + KDLN     LI    G  KY+ A    + VV   W+   W   +N+   D   E + + +R+  F   +I   GFP+ E + +  ++++ GG    +L    C+H++  + +       K      +   W   +++ GYA
Sbjct:   14 LILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRK--KDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWA-DRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVC-AELDDPECSHVVVDEHTTLTKPEPKNNHTHILKSDWFWYTIQNGYA 213
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Zebrafish
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-060626-1])

HSP 1 Score: 139.813 bits (351), Expect = 5.041e-33
Identity = 77/276 (27.90%), Postives = 141/276 (51.09%), Query Frame = 3 
Query:  111 WYVYFVEDT--KTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            + V FVE    K+ + +QAY A+  +++     +  E+       N    ++F  F   +  + +        P  +L  L+    +P  E P+++ ++ +V V  T  ++E + E+ D + +M G V +DLN S   L++  +G++KY  A+ +G P++   W+   WE +Q  D +    E   E YR P+     + +TG   +ER+ ++++  + G  Y   L +  CTHLI  + +G+K+E A+KW +  V+  WL DS+ KG+  DE +Y
Sbjct:    9 YIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQ--DSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRY 282

HSP 2 Score: 89.7373 bits (221), Expect = 1.482e-17
Identity = 52/178 (29.21%), Postives = 89/178 (50.00%), Query Frame = 2 
Query: 2171 FVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIE-LESVACRFTKFDLALADAAKRFHDNGG---------GSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLIN 2674
            F+L +++    +     +++LGG +     ++   TH++ G P R EKYLAA+AAGKW+LH SY++   A G +++E QY+WG   +S  +E L S+  +  +  LA     K                G+F  W V L  ++ +     R+++SGG  ++P   P      TH  ++
Sbjct: 1272 FLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWG---SSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEPNVIVVQGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVD 1446
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Zebrafish
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-060626-1])

HSP 1 Score: 139.813 bits (351), Expect = 5.621e-33
Identity = 77/276 (27.90%), Postives = 141/276 (51.09%), Query Frame = 3 
Query:  111 WYVYFVEDT--KTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            + V FVE    K+ + +QAY A+  +++     +  E+       N    ++F  F   +  + +        P  +L  L+    +P  E P+++ ++ +V V  T  ++E + E+ D + +M G V +DLN S   L++  +G++KY  A+ +G P++   W+   WE +Q  D +    E   E YR P+     + +TG   +ER+ ++++  + G  Y   L +  CTHLI  + +G+K+E A+KW +  V+  WL DS+ KG+  DE +Y
Sbjct:    9 YIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQ--DSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRY 282

HSP 2 Score: 94.7449 bits (234), Expect = 3.873e-19
Identity = 53/171 (30.99%), Postives = 90/171 (52.63%), Query Frame = 2 
Query: 2171 FVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIE-LESVACRFTKFDLALADAAKRFH--DNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLIN 2674
            F+L +++    +     +++LGG +     ++   TH++ G P R EKYLAA+AAGKW+LH SY++   A G +++E QY+WG   +S  +E L S+  +  +  LA     K      N  G+F  W V L  ++ +     R+++SGG  ++P   P      TH  ++
Sbjct: 1273 FLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWG---SSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVD 1440
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Zebrafish
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-060626-1])

HSP 1 Score: 139.813 bits (351), Expect = 5.621e-33
Identity = 77/276 (27.90%), Postives = 141/276 (51.09%), Query Frame = 3 
Query:  111 WYVYFVEDT--KTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            + V FVE    K+ + +QAY A+  +++     +  E+       N    ++F  F   +  + +        P  +L  L+    +P  E P+++ ++ +V V  T  ++E + E+ D + +M G V +DLN S   L++  +G++KY  A+ +G P++   W+   WE +Q  D +    E   E YR P+     + +TG   +ER+ ++++  + G  Y   L +  CTHLI  + +G+K+E A+KW +  V+  WL DS+ KG+  DE +Y
Sbjct:    9 YIVKFVESNGQKSDVALQAYKAIVELQSEKYVQMVDEEAALELDHNDKSLFVFSDFTSSAFDHCRRLGCRIVSPLVVLFCLQKQRCVPKAEQPVYNMSMADVTVSCTNLDKEARSEVMDLIQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAASLGKPILLPSWVKACWEKSQ--DSVFHHSELNTEDYRCPVLKGCTVCVTGLSTVERKEVQRLCDQNGATYTGQLKMNECTHLIVNEPTGQKYEFARKWNVYCVSLHWLFDSIEKGFCQDESRY 282

HSP 2 Score: 94.7449 bits (234), Expect = 3.873e-19
Identity = 53/171 (30.99%), Postives = 90/171 (52.63%), Query Frame = 2 
Query: 2171 FVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIE-LESVACRFTKFDLALADAAKRFH--DNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLIN 2674
            F+L +++    +     +++LGG +     ++   TH++ G P R EKYLAA+AAGKW+LH SY++   A G +++E QY+WG   +S  +E L S+  +  +  LA     K      N  G+F  W V L  ++ +     R+++SGG  ++P   P      TH  ++
Sbjct: 1273 FLLSSLNPQERIDYSHLIEELGGVVLEKQSFDPSCTHVIVGHPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFIQEDQYEWG---SSSILEALPSINSQQKRLALAAMRWRKTLQGCSNKEGAFGGWIVMLNIDQAREAGFRRLLQSGGAKVLPDPSPSLFKETTHLFVD 1440
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Zebrafish
Match: ect2 (epithelial cell transforming 2 [Source:ZFIN;Acc:ZDB-GENE-040831-2])

HSP 1 Score: 94.3597 bits (233), Expect = 3.476e-19
Identity = 71/263 (27.00%), Postives = 124/263 (47.15%), Query Frame = 3 
Query:  177 GMENANLSWIKTEDILQTSTFNKHCSY----IFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKI--LISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
             +++ N   IKT+++       ++C +    +   F+     +L  N     GP  ++      E LP    PL+S  + N+ +CFTGF +  K E+   V ++H   GT+ KD + SAK+  LI+ S    KYR A  +G P+++ DWI K WE   + +     +E +  ++++P F +  +S  GF   E+  +E+   K GG +     VG   CTHL+  + S ++       ++  V  +W   S++      E  Y
Sbjct:   69 ALQDLNTPCIKTDNVCDFGD-GENCEFETVFVLKDFQAPEYSFLYKNDSRILGPPAVMHCANKGEPLPFSSRPLYSMTMLNLSLCFTGFRK--KDEVVALVNLVHHMGGTIRKDFD-SAKVTHLIARSTHGEKYRLAVCMGTPILTPDWIRKAWEHRDDIN-FHAGNELFRTEFKVPPFQDCVLSFLGFSEEEKNNMEERTQKHGGRFQ---AVGDERCTHLVVEENSIKELPFTPSKRLYVVKQEWFWGSIQMDARAGESMY 323
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Zebrafish
Match: ect2 (epithelial cell transforming 2 [Source:ZFIN;Acc:ZDB-GENE-040831-2])

HSP 1 Score: 94.3597 bits (233), Expect = 3.975e-19
Identity = 71/263 (27.00%), Postives = 124/263 (47.15%), Query Frame = 3 
Query:  177 GMENANLSWIKTEDILQTSTFNKHCSY----IFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKI--LISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
             +++ N   IKT+++       ++C +    +   F+     +L  N     GP  ++      E LP    PL+S  + N+ +CFTGF +  K E+   V ++H   GT+ KD + SAK+  LI+ S    KYR A  +G P+++ DWI K WE   + +     +E +  ++++P F +  +S  GF   E+  +E+   K GG +     VG   CTHL+  + S ++       ++  V  +W   S++      E  Y
Sbjct:   69 ALQDLNTPCIKTDNVCDFGD-GENCEFETVFVLKDFQAPEYSFLYKNDSRILGPPAVMHCANKGEPLPFSSRPLYSMTMLNLSLCFTGFRK--KDEVVALVNLVHHMGGTIRKDFD-SAKVTHLIARSTHGEKYRLAVCMGTPILTPDWIRKAWEHRDDIN-FHAGNELFRTEFKVPPFQDCVLSFLGFSEEEKNNMEERTQKHGGRFQ---AVGDERCTHLVVEENSIKELPFTPSKRLYVVKQEWFWGSIQMDARAGESMY 323
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314])

HSP 1 Score: 121.324 bits (303), Expect = 4.716e-34
Identity = 123/505 (24.36%), Postives = 211/505 (41.78%), Query Frame = 3 
Query:  396 ALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCV------------------------IQFLDCEPEFVEKWSNLITEFGGEACKNLNDL-----SVVF------VTHIIVGDA-SKLKE---KEDDITYV-NGDWLQACLDGKAL--------SDSKQFAIEIPKSP----IAVPIS------NPIPDLSINWDLMDQYNLGNVS-----NPFDXXXXXXXXXXXXXXXFGIFHKYRFFLHNLSRVQIKAVSDLIINNSGIINCSPDG--ASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLLSIESDFMFT 1715
            A+ +V +  T  ++E ++++ + V +M G V +DLN S   LI+  +G++KY  A+ +  P++   W+  +W+  Q+   +    +  +++Y  PIF    I +TG   L+R+ ++++    GG Y   L +   THLI  +A G+K+ECA+KW +  V+ QW  DS+ KG+  DE  Y++     +  +  T + + ++   + + I D    + I   CV                        I  L    + ++     +  FGG     L  L      V F      VTHIIVG+   +LK+   K     YV    WL        L        S  +Q  +  P  P     A  IS      N   +   + DL+ QY   N +     N     S+    +       G+F + +F +          + D+I  N+G +  S     A Y V+    C         +VT  +L  C++ ++LL   S+ +FT
Sbjct:    2 AMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWD--QSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCVNESAFNSVMTSRLDPPADALENLDISSLQAPEDLLDGCRIYLCGFGGRKLDKLRRLINSGGGVRFSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQPENIYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLSKNYNMTSCTSQSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLSSQKRAIADYAVVPLLGCEV-ESTVGEVVTNAWLGMCIEQEKLLDPHSNALFT 503

HSP 2 Score: 45.0542 bits (105), Expect = 4.716e-34
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 2 
Query: 1817 GATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDC 1975
            GA VQ+   RK  P K++FA+THL++   +  K+ ++K WN+PAVT  W+  C
Sbjct:  540 GAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQC 592

HSP 3 Score: 88.5817 bits (218), Expect = 3.842e-17
Identity = 49/161 (30.43%), Postives = 84/161 (52.17%), Query Frame = 2 
Query: 2162 EKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRF--------HDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            E+ F L ++D    +   + ++++GG +     ++   TH++ G P R EKYLA++AAGKWVLH SY++   AA ++++E  Y+WG         + S           LA+AA R+         + G G+F  W+V L  ++ K     R+++SGG  +
Sbjct: 1086 ERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSK------SILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIGAFSGWKVILNVDQTKEPGFKRLLQSGGAKV 1240

HSP 4 Score: 52.373 bits (124), Expect = 4.522e-6
Identity = 24/62 (38.71%), Postives = 30/62 (48.39%), Query Frame = 3 
Query:  774 NPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIP 959
            NP   +   THL+   A G K+E AKKW +  V  +WLL   R G   DE  Y +  R   P
Sbjct:  552 NPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSRGSRP 613
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314])

HSP 1 Score: 135.191 bits (339), Expect = 2.098e-31
Identity = 74/269 (27.51%), Postives = 139/269 (51.67%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCV 1061
            YI   F      +LK       GPQ ++  + +   +P  E P+++ A+ +V +  T  ++E ++++ + V +M G V +DLN S   LI+  +G++KY  A+ +  P++   W+  +W+  Q+   +    +  +++Y  PIF    I +TG   L+R+ ++++    GG Y   L +   THLI  +A G+K+ECA+KW +  V+ QW  DS+ KG+  DE  Y++     +  +  T + + ++   + + I D    + I   CV
Sbjct:   58 YICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWD--QSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCV 324

HSP 2 Score: 86.2705 bits (212), Expect = 2.119e-16
Identity = 50/163 (30.67%), Postives = 84/163 (51.53%), Query Frame = 2 
Query: 2162 EKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFH-------DNGG---GSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            E+ F L ++D    +   + ++++GG +     ++   TH++ G P R EKYLA++AAGKWVLH SY++   AA ++++E  Y+WG         + S           LA+AA R+         N G   G+F  W+V L  ++ K     R+++SGG  +
Sbjct: 1219 ERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSK------SILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKV 1375
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314])

HSP 1 Score: 135.191 bits (339), Expect = 2.174e-31
Identity = 74/269 (27.51%), Postives = 139/269 (51.67%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCV 1061
            YI   F      +LK       GPQ ++  + +   +P  E P+++ A+ +V +  T  ++E ++++ + V +M G V +DLN S   LI+  +G++KY  A+ +  P++   W+  +W+  Q+   +    +  +++Y  PIF    I +TG   L+R+ ++++    GG Y   L +   THLI  +A G+K+ECA+KW +  V+ QW  DS+ KG+  DE  Y++     +  +  T + + ++   + + I D    + I   CV
Sbjct:   32 YICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWD--QSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCV 298

HSP 2 Score: 82.8037 bits (203), Expect = 2.541e-15
Identity = 48/158 (30.38%), Postives = 81/158 (51.27%), Query Frame = 2 
Query: 2177 LCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFH-------DNGG---GSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            L ++D    +   + ++++GG +     ++   TH++ G P R EKYLA++AAGKWVLH SY++   AA ++++E  Y+WG         + S           LA+AA R+         N G   G+F  W+V L  ++ K     R+++SGG  +
Sbjct: 1195 LSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSK------SILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKV 1346
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314])

HSP 1 Score: 134.806 bits (338), Expect = 2.886e-31
Identity = 74/269 (27.51%), Postives = 139/269 (51.67%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCV 1061
            YI   F      +LK       GPQ ++  + +   +P  E P+++ A+ +V +  T  ++E ++++ + V +M G V +DLN S   LI+  +G++KY  A+ +  P++   W+  +W+  Q+   +    +  +++Y  PIF    I +TG   L+R+ ++++    GG Y   L +   THLI  +A G+K+ECA+KW +  V+ QW  DS+ KG+  DE  Y++     +  +  T + + ++   + + I D    + I   CV
Sbjct:   58 YICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWD--QSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCV 324

HSP 2 Score: 86.2705 bits (212), Expect = 1.933e-16
Identity = 50/163 (30.67%), Postives = 84/163 (51.53%), Query Frame = 2 
Query: 2162 EKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFH-------DNGG---GSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            E+ F L ++D    +   + ++++GG +     ++   TH++ G P R EKYLA++AAGKWVLH SY++   AA ++++E  Y+WG         + S           LA+AA R+         N G   G+F  W+V L  ++ K     R+++SGG  +
Sbjct: 1225 ERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSK------SILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKV 1381
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Xenopus
Match: ppp1r14b (protein phosphatase 1 regulatory inhibitor subunit 14B [Source:Xenbase;Acc:XB-GENE-485314])

HSP 1 Score: 134.42 bits (337), Expect = 3.305e-31
Identity = 74/269 (27.51%), Postives = 139/269 (51.67%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCV 1061
            YI   F      +LK       GPQ ++  + +   +P  E P+++ A+ +V +  T  ++E ++++ + V +M G V +DLN S   LI+  +G++KY  A+ +  P++   W+  +W+  Q+   +    +  +++Y  PIF    I +TG   L+R+ ++++    GG Y   L +   THLI  +A G+K+ECA+KW +  V+ QW  DS+ KG+  DE  Y++     +  +  T + + ++   + + I D    + I   CV
Sbjct:   58 YICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWD--QSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCV 324

HSP 2 Score: 86.2705 bits (212), Expect = 1.965e-16
Identity = 50/163 (30.67%), Postives = 84/163 (51.53%), Query Frame = 2 
Query: 2162 EKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFH-------DNGG---GSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            E+ F L ++D    +   + ++++GG +     ++   TH++ G P R EKYLA++AAGKWVLH SY++   AA ++++E  Y+WG         + S           LA+AA R+         N G   G+F  W+V L  ++ K     R+++SGG  +
Sbjct: 1262 ERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSK------SILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKV 1418
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Mouse
Match: Topbp1 (topoisomerase (DNA) II binding protein 1 [Source:MGI Symbol;Acc:MGI:1920018])

HSP 1 Score: 135.961 bits (341), Expect = 9.045e-32
Identity = 88/300 (29.33%), Postives = 146/300 (48.67%), Query Frame = 3 
Query:  138 KTPLLVQAYNALDGMEN-----ANLSWIKTEDILQTST--------FNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRA---YIPGLSQTDSSSN 989
            + P LV+   + D  E       ++  +++ED LQ  T         N    YI   F      +LK       GPQ +   +R+ + +P  E P+++  + +V V  T  +++ ++E+   V MM G V +DLN S   LI+  +G++KY  A+ +  P++   WI  +WE +Q        D   +E ++ PIF    I +TG   + R+ ++++  K GG Y   L +  CTHLI  +  G+K+ECA++W +  V  QW  DS+ KG+  DE  Y+   R     +P  S   + SN
Sbjct:    6 QEPFLVKFLKSSDNSECFFKALESIKELQSEDYLQIITDEEALKIRENDKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTD-VNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAETRVEAKMVPDTSTPTAQSN 304

HSP 2 Score: 90.5077 bits (223), Expect = 8.160e-18
Identity = 55/186 (29.57%), Postives = 94/186 (50.54%), Query Frame = 2 
Query: 2081 KASEEIDTRPISKRKICEGAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNG-----GGSFQDWRVCLVEEREKFEKMSRIIESGGGTIV 2623
            + +++I+T+  + RK+         K + +F + +++    +     +K LGG++      +   TH+V G P R EKYLA++AAGKWVLH SY+D    AGR+++E  Y+WG   +S    L  V     K  LA     KR   +       G+F  W+  L  +R +     R++++GG  ++
Sbjct: 1245 QPADKIETQEETHRKV---------KKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGS--SSILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVL 1419
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Mouse
Match: Ect2 (ect2 oncogene [Source:MGI Symbol;Acc:MGI:95281])

HSP 1 Score: 90.5077 bits (223), Expect = 5.484e-18
Identity = 65/225 (28.89%), Postives = 110/225 (48.89%), Query Frame = 3 
Query:  321 GPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDS 980
            GP  +L+  +  E LP    PL+ T++ N+ +CFTGF +  K+EL   V ++H   G + K+ N+    L++      K+R A  +G P++  +WI K WE  +N      A + +  ++++P F +  +S  GF   E+  +E++    GG Y P   VG   CTHLI  + + +        K+  V  +W   S++      E  Y L  +A  P L ++ S
Sbjct:  119 GPPVILNCAQRGEPLPFSCRPLYCTSMLNLVLCFTGFRK--KEELVKLVTLVHHMGGVIRKECNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWE-RRNEQCFCAAVDDFRNEFKVPPFQDCILSFLGFSDEEKHSMEEMTEMQGGSYLP---VGDERCTHLIVEENTVKDLPFEPSKKLFVVKQEWFWGSIQMDARAGETMY-LYEKANTPELKKSVS 336
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Mouse
Match: Ect2 (ect2 oncogene [Source:MGI Symbol;Acc:MGI:95281])

HSP 1 Score: 90.5077 bits (223), Expect = 5.484e-18
Identity = 65/225 (28.89%), Postives = 110/225 (48.89%), Query Frame = 3 
Query:  321 GPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDS 980
            GP  +L+  +  E LP    PL+ T++ N+ +CFTGF +  K+EL   V ++H   G + K+ N+    L++      K+R A  +G P++  +WI K WE  +N      A + +  ++++P F +  +S  GF   E+  +E++    GG Y P   VG   CTHLI  + + +        K+  V  +W   S++      E  Y L  +A  P L ++ S
Sbjct:  119 GPPVILNCAQRGEPLPFSCRPLYCTSMLNLVLCFTGFRK--KEELVKLVTLVHHMGGVIRKECNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWE-RRNEQCFCAAVDDFRNEFKVPPFQDCILSFLGFSDEEKHSMEEMTEMQGGSYLP---VGDERCTHLIVEENTVKDLPFEPSKKLFVVKQEWFWGSIQMDARAGETMY-LYEKANTPELKKSVS 336
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Mouse
Match: Ect2 (ect2 oncogene [Source:MGI Symbol;Acc:MGI:95281])

HSP 1 Score: 90.8929 bits (224), Expect = 5.580e-18
Identity = 65/225 (28.89%), Postives = 110/225 (48.89%), Query Frame = 3 
Query:  321 GPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDS 980
            GP  +L+  +  E LP    PL+ T++ N+ +CFTGF +  K+EL   V ++H   G + K+ N+    L++      K+R A  +G P++  +WI K WE  +N      A + +  ++++P F +  +S  GF   E+  +E++    GG Y P   VG   CTHLI  + + +        K+  V  +W   S++      E  Y L  +A  P L ++ S
Sbjct:  150 GPPVILNCAQRGEPLPFSCRPLYCTSMLNLVLCFTGFRK--KEELVKLVTLVHHMGGVIRKECNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWE-RRNEQCFCAAVDDFRNEFKVPPFQDCILSFLGFSDEEKHSMEEMTEMQGGSYLP---VGDERCTHLIVEENTVKDLPFEPSKKLFVVKQEWFWGSIQMDARAGETMY-LYEKANTPELKKSVS 367
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Mouse
Match: Slf1 (SMC5-SMC6 complex localization factor 1 [Source:MGI Symbol;Acc:MGI:2145448])

HSP 1 Score: 70.4774 bits (171), Expect = 1.129e-12
Identity = 46/134 (34.33%), Postives = 70/134 (52.24%), Query Frame = 2 
Query: 2276 THLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHD-----NGGGSFQDWRVC-LVEEREKFEKMSRIIESG-GGTIVPLIEPVKELHV 2656
            THL++ R  ++EK+LAA AAGKWVL   YI  S  +GRW++E  Y+WG       IE +S       +   +  A KR+ +        G+F  W+V  LV   ++ + + R++E+G    I+P   P    HV
Sbjct:   50 THLIAERLCKSEKFLAACAAGKWVLTKDYIIHSAKSGRWLDETTYEWGYK-----IEKDS------HYSPQMQSAPKRWREELKRTGAPGAFHRWKVVLLVRADKRSDSLVRVLEAGKANVILPKNSPSGITHV 172
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q800K6|TOB1A_XENLA (DNA topoisomerase 2-binding protein 1-A OS=Xenopus laevis OX=8355 GN=topbp1-A PE=1 SV=2)

HSP 1 Score: 142.124 bits (357), Expect = 8.749e-33
Identity = 78/269 (29.00%), Postives = 140/269 (52.04%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCV 1061
            YI   F   +  +LK       GPQ ++  + N   +P  E P+++ A+ +V +  T  ++E ++++   V +M G V +DLN S   LI+  +G++KY  A+ +  P++   W+ ++WE + N   +  +D    E Y  PIF    I +TG   L+R+ ++++    GG Y   L +   THLI  +A G+K+ECA+KW +  ++ QW  DS+ KG+  DE  Y++   + I  +  T + +  ++  N + + D    + I   CV
Sbjct:   58 YICDPFSGAAFSHLKKLGCRIVGPQVVIFCMENQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHHYVQIMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKELWEKS-NQRIIRYSDVNMTE-YLCPIFRGCTICVTGLSSLDRKEVQRLTALHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCISVQWFFDSIEKGFCQDETMYKIEPASTIKSVPDTSTPTGGNSKPNSRTLYDVSQISNISTSCV 324

HSP 2 Score: 92.0485 bits (227), Expect = 1.940e-17
Identity = 56/181 (30.94%), Postives = 90/181 (49.72%), Query Frame = 2 
Query: 2108 PISKRKICEGAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFH-------DNGG---GSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            P  + K  +     E   E+ F L ++D    +   + +++LGG +     ++   TH+V G P R EKYLA++AAGKWVLH SY++   AA R+++E  Y+WG +       + S           LA+AA R+         N G   G+F  W+V L  ++ K     R+++SGG  +
Sbjct: 1242 PQPREKPVQPFSKEETLKERRFQLSSLDPQERIDYSQLIEELGGVVIEKQCFDPSCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKRFIQEEDYEWGSM------SILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKV 1416
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q7ZZY3|TOB1B_XENLA (DNA topoisomerase 2-binding protein 1-B OS=Xenopus laevis OX=8355 GN=topbp1-B PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 2.853e-32
Identity = 85/314 (27.07%), Postives = 155/314 (49.36%), Query Frame = 3 
Query:  120 YFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCV 1061
            YF++  +    +Q+  +L   E      +K +D          C YI   F   +  +LK       GPQ ++  + +   +P  E P+++ A+ +V +  T  ++E ++++   V +M G V +DLN S   LI+  +G+ KY  A+ +  P++   W+ ++WE + N   +  +D    E Y  PIF    I +TG   L+R+ ++++    GG Y   L +   THLI  +A G+K+ECA+KW +  ++ QW  DS+ KG+  DE  Y++   + I  +  T + +  ++  N + + D    + I   CV
Sbjct:   22 YFLKGYEAMKQIQSDESLQLTEEREALVLKEKD---------KCVYICDPFSGAAFSHLKKLGCRIVGPQVVIFCMESQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHHYVQIMGGCVYRDLNVSVTHLIAGEVGSNKYLVAASLEKPILLPSWVKELWEKS-NQRIIRYSDVNMTE-YLCPIFRGCTICVTGLSSLDRKEVQRLTALHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWIVHCISVQWFFDSIEKGFCQDETMYKIEPASTIKSVPDTSTPTGGNSKPNSRTLYDVSQISNISTSCV 324

HSP 2 Score: 91.6633 bits (226), Expect = 2.300e-17
Identity = 54/167 (32.34%), Postives = 86/167 (51.50%), Query Frame = 2 
Query: 2150 ENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFH-------DNGG---GSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            E   E+ F L ++D    +   + +++LGG +     ++   TH+V G P R EKYLA++AAGKWVLH SY++   AA R+++E  Y+WG +       + S           LA+AA R+         N G   G+F  W+V L  ++ K     R+++SGG  +
Sbjct: 1256 ETLKERRFQLSSLDPQERIDYSQLIEELGGVVIEKQCFDPSCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKRFIQEEDYEWGSM------SILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKV 1416
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q92547|TOPB1_HUMAN (DNA topoisomerase 2-binding protein 1 OS=Homo sapiens OX=9606 GN=TOPBP1 PE=1 SV=3)

HSP 1 Score: 140.198 bits (352), Expect = 3.015e-32
Identity = 121/426 (28.40%), Postives = 189/426 (44.37%), Query Frame = 3 
Query:  195 LSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRA---YIPG---------------LSQTDSSSNNSAGQNDKIICDNKLNTK---------------------IWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGD-ASKLKE---KEDDITYVNG-DWLQACLDGKALSDSKQFAIEIPKSPIAVPISN 1340
            L  I  E+ L+    N    YI   F      +LK       GPQ ++  + +   +P  E P+++  + +V +  T   +E ++E+   V MM G V +DLN S   LI+  +G++KY  A+ +  P++   WI  +WE +Q        D   +E ++ PIF    I +TG   L+R+ ++++ +K GG Y   L +  CTHLI  +  G+K+ECAK+W +  V  QW  DS+ KG+  DE  Y+   R     +P                LS   + SN +A    + IC N LN+K                     +   C I         ++K   LI   GG     LN+     VTH+IVGD   +LK+   K     +V G  WL  C   K    S++  I     P+ +P+S+
Sbjct:   39 LQIITEEEALKIKE-NDRSLYICDPFSGVVFDHLKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTD-INMEDFKCPIFLGCIICVTGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDESIYKTEPRPEAKTMPNSSTPTSQINTIDSRTLSDVSNISNINASCVSESIC-NSLNSKLEPTLENLENLDVSAFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNE----DVTHVIVGDYDDELKQFWNKSAHRPHVVGAKWLLECF-SKGYMLSEEPYIHANYQPVEIPVSH 456

HSP 2 Score: 89.7373 bits (221), Expect = 1.045e-16
Identity = 51/164 (31.10%), Postives = 85/164 (51.83%), Query Frame = 2 
Query: 2150 ENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNG-----GGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVP 2626
            E K + IF L +++    +     ++KLGG +     ++   TH+V G P R EKYLA++AAGKWVLH SY++    AG +++E  Y+WG   +S    L  +  +  +  LA     K+           G+F  W+V L  ++ +     R+++SGG  ++P
Sbjct: 1263 ELKKQYIFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEEDYEWGS--SSILDVLTGINVQQRRLALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSREAGFKRLLQSGGAKVLP 1424
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q6ZQF0|TOPB1_MOUSE (DNA topoisomerase 2-binding protein 1 OS=Mus musculus OX=10090 GN=Topbp1 PE=1 SV=2)

HSP 1 Score: 135.961 bits (341), Expect = 6.327e-31
Identity = 88/300 (29.33%), Postives = 146/300 (48.67%), Query Frame = 3 
Query:  138 KTPLLVQAYNALDGMEN-----ANLSWIKTEDILQTST--------FNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRA---YIPGLSQTDSSSN 989
            + P LV+   + D  E       ++  +++ED LQ  T         N    YI   F      +LK       GPQ +   +R+ + +P  E P+++  + +V V  T  +++ ++E+   V MM G V +DLN S   LI+  +G++KY  A+ +  P++   WI  +WE +Q        D   +E ++ PIF    I +TG   + R+ ++++  K GG Y   L +  CTHLI  +  G+K+ECA++W +  V  QW  DS+ KG+  DE  Y+   R     +P  S   + SN
Sbjct:    6 QEPFLVKFLKSSDNSECFFKALESIKELQSEDYLQIITDEEALKIRENDKSLYICDRFSGTVFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTD-VNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAETRVEAKMVPDTSTPTAQSN 304

HSP 2 Score: 90.5077 bits (223), Expect = 5.708e-17
Identity = 55/186 (29.57%), Postives = 94/186 (50.54%), Query Frame = 2 
Query: 2081 KASEEIDTRPISKRKICEGAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNG-----GGSFQDWRVCLVEEREKFEKMSRIIESGGGTIV 2623
            + +++I+T+  + RK+         K + +F + +++    +     +K LGG++      +   TH+V G P R EKYLA++AAGKWVLH SY+D    AGR+++E  Y+WG   +S    L  V     K  LA     KR   +       G+F  W+  L  +R +     R++++GG  ++
Sbjct: 1245 QPADKIETQEETHRKV---------KKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACKTAGRFVQEEDYEWGS--SSILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVDRPREAGFKRLLQAGGAKVL 1419
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q9H8V3|ECT2_HUMAN (Protein ECT2 OS=Homo sapiens OX=9606 GN=ECT2 PE=1 SV=4)

HSP 1 Score: 93.9745 bits (232), Expect = 3.474e-18
Identity = 69/235 (29.36%), Postives = 112/235 (47.66%), Query Frame = 3 
Query:  321 GPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSS-SNNSAGQNDK 1013
            GP  +L+  +  E LP    PL+ T++ N+ +CFTGF +  K+EL   V ++H   G + KD N+    L++      K+R A  +G P++  +WI K WE     D     D++  E +++P F +  +S  GF   E+  +E++    GG Y P L    CTHL+  +   +        K+  V  +W   S++      E  Y L  +A  P L ++ S  S N+   N K
Sbjct:  150 GPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRK--KEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNE-FKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLP-LGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMY-LYEKANTPELKKSVSMLSLNTPNSNRK 379
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. TrEMBL
Match: A0A430Q6U0 (Topoisomerase (DNA) II binding protein 1 OS=Schistosoma bovis OX=6184 GN=DC041_0001837 PE=4 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 1.946e-66
Identity = 163/621 (26.25%), Postives = 294/621 (47.34%), Query Frame = 3 
Query:  117 VYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTF--NKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQ------------------------LSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGD--ASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAIEIPKSPIAVP--------ISNPIPDLSINWD-----LMDQYNLGNVSN-----------------------PFDXXXXXXXXXXXXXXXFGIFHKYRFFLHN-LSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLL--SIESDFMFTRM--DINPNILTGTILSFSGY 1772
            + FV +  + LL  A+NA+   EN     + + ++++      ++   ++   F+ +   +L+S  +  +GPQ +L Y+R ++ LP    PLFSTAL N     T      +K++ D + M+HG+VS+DL  +  ++++  +G++KY   +   + ++  DWI + W L++  DPLD     + E+YR+PIF  L I ++G  + ER+ +  ++ K GG Y+  + +G  THL+  QASG K+  AKKWKI  ++ +WL+DSV KGYA DE+ Y+                        +S  +Y+   S+ D + +    ++ +++   +  + I   C I+F DC+ + V  +S+++ E GG+ C  L D     +TH++VG   +S    +E  + YV G WL AC D K         + IP+    +P        + + +   S N D     L++QY  G +++                       P  ++  +    +      G F    FF H  LS   +   +D+I    G I+ +    +++V    +   PP      VT+ +++ C+  K L    I  ++ F  +     P  L+G ++S SG+
Sbjct:    4 IIFVNEPPSSLLNAAFNAVSEYENICPKRLSSSEVIKLYPLKPSRDVCFVLARFDGECYDHLRSLGVSIYGPQVILHYVRENKPLPNLPYPLFSTALLNATATVTSVTGAQRKQIFDSIQMLHGSVSRDLTDNVNVIVTAKVGSKKYLVGASRNLHILLPDWITEAWRLSEIQDPLDMMLGIYTEKYRVPIFSQLVICVSGLSVEERKEVSDLVSKHGGKYSGVMKIGETTHLVTRQASGTKYVHAKKWKIQIISIKWLIDSVDKGYALDEEDYRVDQVKTKSSTPTPNTQQNEISFDNISAISYVGAASKVDETRSVCFRESAQVV---QSGSSILSGCSIRFSDCKKDEVHLYSSIVRELGGKLCTELFDQVTEMLTHVVVGSTPSSNGMSREKGVKYVKGAWLLACRDSK---------VRIPEDEYLIPEINENLQRLEDEVDVTSTNIDAADFQLINQYFTGGLADDEFQDEAIETKEAIQDFAKTTIRPEIADEVKHPEVSEISYPGGCFSNLIFFFHKELSDADVVKYTDIIKTAGGCISDNERNVNFLVAPFFVISLPPISSCEFVTIGWISYCIHEKSLCLQEIRKEYAFKPVLHKPGPPPLSGCVISLSGF 612

HSP 2 Score: 45.4394 bits (106), Expect = 1.946e-66
Identity = 29/64 (45.31%), Postives = 43/64 (67.19%), Query Frame = 2 
Query: 1784 RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDC 1975
            RSLL S   A GA VQ+   RK +  +N+ A+THLI +KPD +K+P+++ W +PAV + W+  C
Sbjct:  617 RSLLTSYAQALGAVVQECFLRKSVASRNLAASTHLIAAKPDGRKWPAAQQWGLPAVNRLWLYKC 680

HSP 3 Score: 97.4413 bits (241), Expect = 1.181e-16
Identity = 66/204 (32.35%), Postives = 102/204 (50.00%), Query Frame = 2 
Query: 2216 EAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFI-ELESVAC----------RFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELH-VTH--FLINKNLCSGLPDLT-SHSCYEIDLIPFYLSARNDCERKKF 2782
            E V++LGG ++     +   THL+   P R+EK L  +A+GKW+LH SY+D       W++E  Y+WGG  T   + +L    C          + T     LA +A+R+  +GG +F +W+V      +K     RIIE+GGG ++    P    H VTH  F    N  S L +L  ++S   ID +  YLS+  +  + +F
Sbjct: 1262 EIVERLGGEVDNLTTLSERTTHLIVYAPTRSEKCLICLASGKWMLHKSYLDACSRESCWVDEAAYEWGGPGTEPLLMQLSPFPCPPGGPNILHQQKTAQIRDLARSARRWRLSGGKAFHNWKVIFGPGCDKESSFRRIIEAGGGKVLSSNPPYPPPHEVTHAFFKTGSNSTSALSNLPRNYSWLRIDYLCSYLSSGQEISKSEF 1465
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. TrEMBL
Match: A0A183KJ35 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS15042 PE=4 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 1.106e-65
Identity = 162/621 (26.09%), Postives = 293/621 (47.18%), Query Frame = 3 
Query:  117 VYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTF--NKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQ------------------------LSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGD--ASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAIEIPKSPIAVP--------ISNPIPDLSINWD-----LMDQYNLGNVSN-----------------------PFDXXXXXXXXXXXXXXXFGIFHKYRFFLHN-LSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLL--SIESDFMFTRM--DINPNILTGTILSFSGY 1772
            + FV +  + LL  A+NA+   EN     + + ++++      ++   ++   F+ +   +L+S  +  +GPQ +L Y+R ++ LP    PLFSTAL N     T      +K++ D + M+HG+VS+DL  +  ++++  +G++KY   +   + ++  DWI + W L++  DPLD     + E+YR+PIF  L I ++G  + ER+ +  ++ K GG Y+  + +G  THL+  QASG K+  AKKWKI  ++ +WL+DSV KGYA DE+ Y+                        +S  +Y+   S+ D + +    ++ +++   +  + I   C I+F DC+ + V  +S+++ E GG+ C  L D     +TH++VG   +S    +E  + YV G WL AC D K         + IP+    +P        + + +   S N D     L++QY  G +++                       P  ++  +    +      G F    F  H  LS   +   +D+I    G I+ +    +++V    +   PP      VT+ +++ C+  K L    I  ++ F  +     P  L+G ++S SG+
Sbjct:    4 IIFVNEPPSSLLNAAFNAVSEYENICPKRLSSSEVIKLYPLKPSRDVCFVLARFDGECYDHLRSLGVSIYGPQVILHYVRENKPLPNLPYPLFSTALLNATATVTSVTGAQRKQIFDSIQMLHGSVSRDLTDNVNVIVTAKVGSKKYLVGASRNLHILLPDWITEAWRLSEIQDPLDMMLGIYTEKYRVPIFSQLVICVSGLSVEERKEVSDLVSKHGGKYSGVMKIGETTHLVTRQASGTKYVHAKKWKIQIISIKWLIDSVDKGYALDEEDYRVDQVKTKSSTPTPNTQQNEISFDNISAISYVGAASKVDETRSVCFRESAQVV---QSGSSILSGCSIRFSDCKKDEVHLYSSIVRELGGKLCTELFDQVTEMLTHVVVGSTPSSNGMSREKGVKYVKGAWLLACRDSK---------VRIPEDEYLIPEINENLQRLEDEVDVTSTNIDAADFQLINQYFTGGLADDEFQDEAIETKEAIQDFAKTTIRPEIADEVKHPEVSEISYPGGCFSNLIFSFHKELSDADVVKYTDIIKTAGGCISDNERNVNFLVAPFFVISLPPISSCEFVTIGWISYCIHEKSLCLQEIRKEYAFKPVLHKPGPPPLSGCVISLSGF 612

HSP 2 Score: 44.669 bits (104), Expect = 1.106e-65
Identity = 29/64 (45.31%), Postives = 43/64 (67.19%), Query Frame = 2 
Query: 1784 RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDC 1975
            RSLL S   A GA VQ+   RK +  +N+ A+THLI +KPD +K+P+++ W +PAV + W+  C
Sbjct:  617 RSLLTSYAQALGAVVQECFLRKSVASRNLAASTHLIAAKPDGRKWPAAQQWGLPAVNRLWLYKC 680
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. TrEMBL
Match: A0A183NWR5 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS6551 PE=4 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 5.200e-65
Identity = 160/621 (25.76%), Postives = 292/621 (47.02%), Query Frame = 3 
Query:  117 VYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTF--NKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQ------------------------LSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGD--ASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAIEIPKSPIAVP--------ISNPIPDLSINWD-----LMDQYNLGNVSN-----------------------PFDXXXXXXXXXXXXXXXFGIFHKYRFFLHN-LSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLL--SIESDFMFTRM--DINPNILTGTILSFSGY 1772
            + FV +  + LL  A+NA+   EN     + + ++++      ++   ++   F+ +   +L+S  +  +GPQ +L Y+R ++ LP    PLFSTAL N     T      +K++ D + M+HG VS+DL  +  ++++  +G++KY   +   + ++  DWI + W L++  DP+D     + E+YR+PIF  L I ++G  + ER+ +  ++ K GG Y+  + +G  THL+  QASG K+  AKKWKI  ++ +WL+DSV KGYA DE+ Y+                        +S  +Y+   S+ D + +    ++ +++   +  + I   C I+F DC+ + V  +S+++ E GG+ C  L D     +TH++VG   +S    +E  + Y+ G WL AC D K         + IP+    +P        + + +   S N D     L++QY  G +++                       P  ++  +    +      G F    F  H  LS   +   +D+I    G I+ +    +++V    +   PP      VT+ +++ C+  K L    I  ++ F  +     P  L+G ++S SG+
Sbjct:    4 IIFVNEPPSSLLNAAFNAVSEYENICPKRLSSSEVIKLYPLKPSRDVCFVLARFDGECYDHLRSLGVSIYGPQVILHYVRENKPLPNLPYPLFSTALLNATATVTSVTGAQRKQIFDSIQMLHGCVSRDLTDNVNVIVTAKVGSKKYLVGASRNLQILLPDWITEAWRLSEIQDPIDMMLGIYTEKYRVPIFSQLVICVSGLSVEERKEVSDLVSKHGGKYSGVMKIGETTHLVTRQASGTKYVHAKKWKIQIISIKWLIDSVDKGYALDEEDYRVDQVKTKSSTPTPNTQQNEISFDNISAISYVGAASKVDETRSVCFRESAQVV---QSGSSILSGCSIRFSDCKKDEVHLYSSIVRELGGKLCTELFDQVTEMLTHVVVGSTPSSNGMSREKGVKYIKGAWLLACRDSK---------VRIPEDEYLIPEINENFQRLEDEVDVTSTNIDAADFQLINQYFTGGLADDEFQDEAIETKEAIQDFAKTTIRPETADEVKHPEVSETSYPGGCFSNLVFSFHKELSDADVVKYTDIIKTAGGCISDNERNVNFLVAPFFVINLPPISSCEFVTIGWISYCIHEKSLCLQEIRKEYAFKPVLHKPGPPPLSGCVISLSGF 612

HSP 2 Score: 44.2838 bits (103), Expect = 5.200e-65
Identity = 28/64 (43.75%), Postives = 43/64 (67.19%), Query Frame = 2 
Query: 1784 RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDC 1975
            R+LL S   A GA VQ+   RK +  +N+ A+THLI +KPD +K+P+++ W +PAV + W+  C
Sbjct:  617 RNLLTSYAQALGAVVQECFLRKSVASRNLAASTHLIAAKPDGRKWPAAQQWGLPAVNRLWLYKC 680

HSP 3 Score: 85.1149 bits (209), Expect = 8.016e-13
Identity = 49/146 (33.56%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 2216 EAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFI-ELESVAC----------RFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            E V++LGG ++     +   THL+   P R+EK L  +A+GKW+LH SY+D       W++E  Y+WGG  T   + +L    C          + T     LA +A+R+  +GG +F +W+V      +K     RIIE+GGG +
Sbjct: 1186 EIVERLGGEVDNLTTLSERTTHLIVYAPTRSEKCLICLASGKWMLHKSYLDACSRESCWVDEAAYEWGGPGTEPLLMQLSPFPCPPGGPNILHQQKTAQIRDLARSARRWRLSGGKAFHNWKVIFGPGCDKESSFRRIIEAGGGKV 1331
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. TrEMBL
Match: A0A183LAC4 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS749 PE=4 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 5.938e-65
Identity = 160/621 (25.76%), Postives = 294/621 (47.34%), Query Frame = 3 
Query:  117 VYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTF--NKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQ------------------------LSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGD--ASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAIEIPKSPIAVP--------ISNPIPDLSINWD-----LMDQYNLGNVSN-----------------------PFDXXXXXXXXXXXXXXXFGIFHKYRFFLHN-LSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLL--SIESDFMFTRM--DINPNILTGTILSFSGY 1772
            + FV +  + LL  A+NA+   EN     + + ++++      ++   ++   F+ +   +L+S  +  +GPQ +L Y+R ++ LP    PLFSTAL N     T      +K++ D + M+HG+VS+DL  +  ++++  +G++KY   +   + ++  DWI + W L++  DP+D     + E+YR+PIF  L I ++G  + ER+ +  ++ K GG Y+  + +G  THL+  QASG K+  AKKWKI  ++ +WL+DSV KGYA DE+ Y+                        +S  +Y+   S+ D + +  + ++ +++   +  + I   C I+F DC+ + V  +S+++ E GG+ C  L D     +TH++VG   +S    +E  + Y+ G WL AC D K         + IP+    +P        + + +   S N D     L++QY  G +++                       P  ++  +    +      G F    F  H  LS   +   +D+I    G I+ +    +++V    +   PP      VT+ +++ C+  K L    I  ++ F  +     P  L+G ++S SG+
Sbjct:    4 IIFVNEPPSSLLNAAFNAVSEYENICPKRLSSSEVIKLYPLKPSRDVCFVLARFDGECYDHLRSLGVSIYGPQVILHYVRENKPLPNLPYPLFSTALLNATATVTSVTGAQRKQIFDSIQMLHGSVSRDLTDNVNVIVTAKVGSKKYLVGASRNLHILLPDWITEAWRLSEIQDPIDMMLGIYTEKYRVPIFSQLVICVSGLSVEERKEVSDLVSKHGGKYSGVMKIGETTHLVTRQASGTKYVHAKKWKIQIISIKWLIDSVDKGYALDEEDYRVDQAKTKSSTPTPNTQQNEISFDNISAISYVGAASKVDETRSVCSRESAQVV---QSGSSILSGCSIRFSDCKKDEVHLYSSIVRELGGKLCTELFDQVTEMLTHVVVGSTPSSNGMSREKGVKYIKGAWLLACRDSK---------VRIPEDEYLIPEINENLQRLEDEVDVTSTNIDAADFQLINQYFTGGLADDEFQDEAIETKEAIQDFSKTTIRPEIADEVKYPEVSEISYPGGCFSNLVFSFHKELSDADVVKYTDIIKTAGGCISDNERNVNFLVTPFFVINLPPISSCEFVTIGWISYCIHEKSLCLQEIRKEYAFKPVLHKPGPPPLSGCVISLSGF 612

HSP 2 Score: 43.1282 bits (100), Expect = 5.938e-65
Identity = 28/64 (43.75%), Postives = 42/64 (65.62%), Query Frame = 2 
Query: 1784 RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDC 1975
            RSLL S     GA VQ+   RK +  +N+ A+THLI +KPD +K+P+++ W +PAV + W+  C
Sbjct:  617 RSLLTSYAQDLGAVVQECFLRKSVASRNLAASTHLIAAKPDGRKWPAAQQWGLPAVNRLWLYKC 680

HSP 3 Score: 85.1149 bits (209), Expect = 8.023e-13
Identity = 49/146 (33.56%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 2216 EAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFI-ELESVAC----------RFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTI 2620
            E V++LGG ++     +   THL+   P R+EK L  +A+GKW+LH SY+D       W++E  Y+WGG  T   + +L    C          + T     LA +A+R+  +GG +F +W+V      +K     RIIE+GGG +
Sbjct: 1189 EIVERLGGEVDNLTTLSERTTHLIVYAPTRSEKCLICLASGKWMLHKSYLDACSRESCWVDEAAYEWGGPGTEPLLMQLSPFPCPPGGPNILHQQKTAQIRDLARSARRWRLSGGKAFHNWKVIFGPGCDKESSFRRIIEAGGGKV 1334
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. TrEMBL
Match: A0A095AI88 (DNA topoisomerase 2-binding protein 1 OS=Schistosoma haematobium OX=6185 GN=MS3_01849 PE=4 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 6.087e-65
Identity = 161/623 (25.84%), Postives = 291/623 (46.71%), Query Frame = 3 
Query:  117 VYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTF--NKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQ------------------------LSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGD--ASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAIEIPKSPIAVP--------ISNPIPDLSINWD-----LMDQYNLGNVSNPFDXXXXXXXXXXXXXXXF-------------------------GIFHKYRFFLHN-LSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLL--SIESDFMFTRM--DINPNILTGTILSFSGY 1772
            + F+ +  + LL  A+NA+   EN     + + ++++      ++   ++   F+ +   +L+S  +  +GPQ +L Y+R ++ LP    PLFSTAL N     T      +K++ D + M+HG+VS+DL  +  ++++  +G++KY   +   + ++  DWI + W L++  DP+D     + E+YR+PIF  L I ++G  + ER+ +  ++ K GG Y+  + +G  THL+  QASG K+  AKKWKI  ++ +WL+DSV KGYA DE+ Y+                        +S  +Y+   S+ D + +    ++ +++   +  + I   C I+F DC+ + V  +S+++ E GG+ C  L D     +TH++VG   +S    +E  + YV G WL AC D K         + IP+    +P        + + +   S N D     L++QY  G +++  D   +           F                         G F    F  H  LS   +   +D+I    G I+ +    +++V    +   PP      VT+ +++ C+  K L    I  ++ F  +     P  L+G ++S SG+
Sbjct:    4 IIFINEPPSSLLNAAFNAVSEYENICPKRLSSSEVIKLYPLKPSRDVCFVLARFDGECYDHLRSLGVSIYGPQVILHYVRENKPLPNLPYPLFSTALLNATATVTSVTGAQRKQIFDSIQMLHGSVSRDLTDNVNVIVTAKVGSKKYLVGASRNLHILLPDWITEAWRLSEIQDPIDMMLGIYTEKYRVPIFSQLVICVSGLSVEERKEVSDLVSKHGGKYSGVMKIGETTHLVTRQASGTKYVHAKKWKIQIISIKWLIDSVDKGYALDEEDYRVDQVKTKSSTPTPNTQQNEISFDNISAISYVGAASKVDETRSVCFRESAQVV---QSGSSILSGCSIRFSDCKKDEVHLYSSIVRELGGKLCTELFDQVTEMLTHVVVGSTPSSNGMSREKGVKYVKGAWLLACRDSK---------VRIPEDEYLIPEINENLQRLEDEVDVTSTNIDAADFQLINQYFTGGLAD--DEFQDEATETKEAIQDFAKTTIRPEIADEVKHPEVSEISYPGGCFSNLIFSFHKELSDADVVKYTDIIKTAGGCISDNERNVNFLVAPFFVINLPPISSCEFVTIGWISYCIHEKSLCLQEIRKEYAFKPVLHKPGPPPLSGCVISLSGF 612

HSP 2 Score: 45.4394 bits (106), Expect = 6.087e-65
Identity = 29/64 (45.31%), Postives = 43/64 (67.19%), Query Frame = 2 
Query: 1784 RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDC 1975
            RSLL S   A GA VQ+   RK +  +N+ A+THLI +KPD +K+P+++ W +PAV + W+  C
Sbjct:  617 RSLLTSYAQALGAVVQECFLRKSVASRNLAASTHLIAAKPDGRKWPAAQQWGLPAVNRLWLYKC 680

HSP 3 Score: 97.0561 bits (240), Expect = 1.708e-16
Identity = 66/204 (32.35%), Postives = 102/204 (50.00%), Query Frame = 2 
Query: 2216 EAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFI-ELESVAC----------RFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELH-VTH--FLINKNLCSGLPDLT-SHSCYEIDLIPFYLSARNDCERKKF 2782
            E V++LGG ++     +   THL+   P R+EK L  +A+GKW+LH SY+D       W++E  Y+WGG  T   + +L    C          + T     LA +A+R+  +GG +F +W+V      +K     RIIE+GGG ++    P    H VTH  F    N  S L +L  ++S   ID +  YLS+  +  + +F
Sbjct: 1185 EIVERLGGEVDNLTTLSERTTHLIVYAPTRSEKCLICLASGKWMLHKSYLDACSRESCWVDEAAYEWGGPGTEPLLMQLSPFPCPPGGPNILHQQKTAQIRDLARSARRWRLSGGKAFHNWKVIFGPGCDKESSFRRIIEAGGGKVLSSNPPYPPPHEVTHAFFKTGGNSTSALSNLPRNYSWLRIDYLCSYLSSGQEISKSEF 1388
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Cavefish
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:NCBI gene;Acc:103036620])

HSP 1 Score: 140.584 bits (353), Expect = 2.861e-33
Identity = 80/276 (28.99%), Postives = 140/276 (50.72%), Query Frame = 3 
Query:  111 WYVYFVEDT--KTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            + V FVE    K  L  +AY A+  +++        E+       N    Y+F  F   +  + +        P  +L  L+    +P  E P+++ A+ +V V  T  ++EV+ ++ D V +M G V +DLN S   L++  +G++KY  A+ +G PV+   W+   WE +Q  D +    +  +E Y  P+F    + +TG   +ER+ ++++    GG Y   L +  CTHLI  + SG+K+E A+KW +  ++  WL DS+ KG+  +E +Y
Sbjct:    9 FMVKFVEHNGQKRELGQKAYEAIVELQSEKYVQTIGEEAALKLDHNDKSLYVFSDFTSSAFEHCRKLGCRIVSPLVVLFCLQQQRCVPKAEQPVYNMAMADVTVTCTSLDKEVRTDVMDLVQLMGGRVYRDLNVSVTHLVAGEVGSKKYLVAANLGKPVLLPTWVKACWEKSQ--DSMFRYSDLRVEDYLCPVFKGCTVCVTGLSTVERKEVQRLCELHGGTYTGQLKMNECTHLIVSEPSGQKYEFARKWNVYCISVHWLFDSIEKGFCQEESRY 282

HSP 2 Score: 80.1073 bits (196), Expect = 1.125e-14
Identity = 64/245 (26.12%), Postives = 105/245 (42.86%), Query Frame = 2 
Query: 1988 EMLSTDLYSLELLANSVDKGKTEINHGVRKRKASEEIDTRPISKRKICEGAELIE--------NKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLR--------TSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLIN 2674
            EM + D+   ++    V   +  ++  V K   +  I   PISK  +    ++ E         K    F L ++     +     +++LGG +     ++   +H++ G P R EKYLAA+AAGKW+LH SY++   A G ++ E  Y+WG           TS+   L   A R+ K     AD          G+F  W V L  +  +     R+++SGG  ++P   P      TH   +
Sbjct: 1211 EMAACDVIEAQMRPK-VTPPRVNVDQRVPKTPEAPSI-AFPISKPAVPPAPQIEEEEETEQEVKKDPPRFQLSSLSPQERIDYSHVIEELGGIVLEKQCFDPSCSHIIVGYPLRNEKYLAAMAAGKWILHRSYLEACRAEGYFLVEEDYEWGSRSILDAVPTVTSQQKRLALSALRWRKALQGRADKE--------GAFGGWIVMLNIDHARDAGFRRLLQSGGAKVLPGPSPSLYKDTTHLFAD 1445
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Cavefish
Match: ect2 (epithelial cell transforming 2 [Source:ZFIN;Acc:ZDB-GENE-040831-2])

HSP 1 Score: 102.064 bits (253), Expect = 1.204e-21
Identity = 69/231 (29.87%), Postives = 112/231 (48.48%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMH---GTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            ++   F+     YL  N     GP  +L      E LP    PL+ST + N+ +CFTGF +  K+E+ + V ++H   GT+ KD +A    LI+ S    KYR A  +G P+++ DWI K WE     +   G +E+    +++P F +  +S  GF   E+  +E+  +K GG +   L VG   CTHL+  + S ++       ++  V  +W   S++      E  Y
Sbjct:   67 FVLKDFQSPYYSYLYKNDNRILGPPVILHCAGKGEPLPFSSRPLYSTTMLNMSLCFTGFRK--KEEVVNLVSLVHHMGGTIRKDFSAKVTHLIAHSTHGEKYRLAVCMGTPILTPDWIRKAWEHKDEINFHAGVEEFRT-AFKVPPFQDCVLSFLGFSEEEKNNMEERTLKHGGQF---LEVGDEKCTHLVVEENSIKELPFVPSKRLYVVKQEWFWGSIQMDARAGESMY 291
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Cavefish
Match: slf1 (SMC5-SMC6 complex localization factor 1 [Source:ZFIN;Acc:ZDB-GENE-141216-402])

HSP 1 Score: 57.3806 bits (137), Expect = 8.104e-8
Identity = 39/139 (28.06%), Postives = 64/139 (46.04%), Query Frame = 2 
Query: 2210 LREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVP 2626
            L + +K L G+   G++Y  E THL+      +EK+++A  AGKW++   ++ DS+    W+ E  Y+   L  +      S+     K+   +A           G+FQ W VCL  E      M  I+++G   I P
Sbjct:   22 LLQGIKCLHGSYIGGSEYREEMTHLIVIHASASEKFISA-CAGKWIVTPEFVLDSVKQNAWLSEALYE---LNLTANTRTPSMTNPLQKWREKVARGVV------SGAFQGWTVCLHIEDPDRRSMFEILKAGKAVIDP 150
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Sea Lamprey
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:ZFIN;Acc:ZDB-GENE-060626-1])

HSP 1 Score: 132.494 bits (332), Expect = 1.563e-31
Identity = 118/493 (23.94%), Postives = 207/493 (41.99%), Query Frame = 3 
Query:  114 YVYFVEDTK--TPLLVQAYNALDGMENANLSWIKTEDILQTSTFNK--HCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQN-----------------------DKIICDNKLNTK----------------------------IWKDCVIQFLDC--EPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVN---------GDWLQACLDGKAL-----------SDSKQFAIEIPKSPIA-VPISNPIPDLS 1358
            +V FVED +  +    +AY  +  +++ +L  ++  D  +  T  +     Y+   F   +  +L        GPQ +L  L  +  +P  E P+++ AL +  +  T  +++V++E+   + +M G V +D  ++   L++  +G++KY  A+ +  P++   WI   WE +Q    + G D  W+E++  P      +S+TG    ER  I++++ K GG Y+ +L +  CTHLI  +  G+K+E A+KW +  V  QWL DSV +G   DE  Y +     I G  Q  S+S  S  +                        ++ +    + T+                            +   C  Q   C    + +EK   L+   GG    N N      VTHIIVGD    + +E D+T V            WL  C   + L           +  ++  ++ P +P+A  P    +P L+
Sbjct:   10 FVKFVEDNENESKYFKEAYEKICKIQSESL--LQRVDAAKVLTLKEKDRSLYVCDPFHGVAFDHLVKMGCRVVGPQVVLFCLDRERCVPKAEHPVYNMALADAVLSCTNLDKDVREEMHRLIGLMGGRVLRDFTSAVTHLVAGEVGSKKYLVAASLKKPIMLVSWITTCWEASQERM-VCGTDNEWLEKHMCPPLQGCMLSVTGLSTQERRDIQRLVTKHGGTYSGELKMNECTHLIVHKPHGQKYEFARKWNVACVTVQWLHDSVERGACQDESLYSVLPLDDIEGGPQEPSTSTPSPSRGRPGSCAMSMSNVSSISNISMSRINETVSTTSMATRLEVMAMGARAEVDELDSLDPAHLYGLDSLLDGCKAQIFVCGFSGKRLEKLRRLVN-LGGGVRYNQNSAD---VTHIIVGD----RGRERDLTLVQENSMRNVVTAKWLVDCFTQRRLLPTELYLHPDYTAPEEILLQAPLAPVASAPSQQRVPPLT 491

HSP 2 Score: 82.0333 bits (201), Expect = 5.332e-16
Identity = 55/185 (29.73%), Postives = 94/185 (50.81%), Query Frame = 2 
Query: 2156 KSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADY-NGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWM-EEYQYQWGGLR--------TSEFIELESVACRFTKFDLALADAAKRFHDNGG--GSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLIN 2674
            +  + F L ++     +   + +++L G + L   Y +   +H++ G P R EKYLA++AAGKWVLH SY++   A GR++ +E +Y+WG           T++   L + A R+ K  L L    +R  + G   G+F  W+V L  E+ K     R+++SGG  ++   E       TH  ++
Sbjct: 1295 QPHRYFQLSSMSAQERIDYLQLIEQLEGGVVLDKQYFDPSCSHIIVGNPLRNEKYLASMAAGKWVLHKSYLEACRAMGRFVQQEEEYEWGSSHILDALANLTTQQRRLATAAMRWRK-KLQL----QRRQEPGAQVGAFGGWKVILNIEQSKESGFRRLLQSGGAEVLSSGEAQALEEATHLFVD 1474

HSP 3 Score: 50.447 bits (119), Expect = 2.653e-6
Identity = 23/53 (43.40%), Postives = 30/53 (56.60%), Query Frame = 3 
Query:  774 NPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            NP   +   THL+  +ASG KFE A KW +  V   WLL+S RKG    E ++
Sbjct:  710 NPKKNMHASTHLLVREASGCKFEAAIKWDLPAVGVGWLLESARKGCRAKEHRH 762
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Nematostella
Match: EDO38946 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBH5])

HSP 1 Score: 147.517 bits (371), Expect = 6.539e-36
Identity = 76/227 (33.48%), Postives = 125/227 (55.07%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQ 935
            YI   FE     YLKS K    GPQC+L  L+   +LP    P+++ +L+ V  C T    + ++ L D + MM GTV +D   S   LI+  +G++KY  A  +  PV+  +W+  +WE  +++  + G D  + ++++ PIF    I +TG     R+ ++++  + GG Y+ +L +  CTHL+  Q  GEK+E A+KW I  V+ QW  D +R G+  +E  Y+
Sbjct:    7 YILDPFEGDHFGYLKSQKCRICGPQCVLSCLKTKTSLPNSSFPVYTASLREVHACCTSVPRKERQHLHDYIHMMGGTVMRDFTESVTHLIAGEVGSKKYSVACSLNKPVMLPEWVFTLWEDGKSSHVV-GTDPAY-DRFKCPIFKGCTICVTGLDAQTRQEVKQLCNQNGGVYSGELNMNTCTHLLVNQPKGEKYEFARKWNIHCVSTQWFYDCIRNGFWVEESGYR 231

HSP 2 Score: 87.8113 bits (216), Expect = 1.589e-17
Identity = 57/175 (32.57%), Postives = 89/175 (50.86%), Query Frame = 2 
Query: 2165 KIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLA----LADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELH-VTHFLIN 2674
            ++  +   +KA Y  L   +++LGG +     +N E TH+V G+P R EKYLAA+AAGKWVLH SY++ S   G ++ E  ++WG     E     + A +  +  LA     AD ++       G+F  W V L  ++ +     R++E+GG  +V    P      VTH  +N
Sbjct: 1180 QLSAMTPQEKADYSLL---IEQLGGKVFDTMYFNPECTHVVVGKPNRNEKYLAAMAAGKWVLHKSYLEASREEGVFVREAPHEWGVEVAGEQPNKLATAAKRWRCRLAHQRKTADPSQSL-----GAFTGWSVFLCVDKSRQPGFKRLLEAGGAKVVSSRPPYSCAEGVTHAFVN 1346
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Nematostella
Match: EDO41864 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2Z1])

HSP 1 Score: 63.1586 bits (152), Expect = 1.780e-11
Identity = 43/166 (25.90%), Postives = 71/166 (42.77%), Query Frame = 2 
Query: 2276 THLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRV-CLVEEREKFEKMSRIIESGGGTIV-----PLIEPVKELHVTHFLINKNLCSGLPDLTSHS--CYEIDLIPFYL 2749
            TH+V  +P R EK+L   A GKW+L   Y++DS AA +W++E  Y+W        +  + V+              ++  +   G F  W V  LV + ++     R++ +G   I+         P     +TH  + K+L   +  L      C   D IP Y+
Sbjct:    5 THIVCNKPNRGEKFLCGCATGKWLLRKEYLEDSFAAQQWLDEELYEWNDACNVPGLRQDHVSAPMR---------WRKLSEKERGPFDGWNVLVLVNDAKRRIAYKRLLAAGQARILNGKPGDTYMPKLAKKLTHVFVEKSLECDVEGLAKAGVPCLSPDYIPEYI 161
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Nematostella
Match: EDO44560 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVE4])

HSP 1 Score: 46.595 bits (109), Expect = 3.391e-6
Identity = 23/64 (35.94%), Postives = 36/64 (56.25%), Query Frame = 2 
Query: 2222 VKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQ 2413
            V+KLGG   +        TH+++G P RT   L AIA G W++   ++  S+ AG W++E  Y+
Sbjct:    7 VRKLGG-FYIADKAGANTTHVIAGSPRRTLNVLRAIAQGCWLVSPEWVMKSLEAGYWVDEEPYE 69
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Medaka
Match: topbp1 (DNA topoisomerase II binding protein 1 [Source:NCBI gene;Acc:101166667])

HSP 1 Score: 143.665 bits (361), Expect = 2.543e-34
Identity = 83/302 (27.48%), Postives = 154/302 (50.99%), Query Frame = 3 
Query:  111 WYVYFVED--TKTPLLVQAYNALDGMENAN-LSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQN 1007
            + V FVE   +     V+AY A+  +++   L  +K E++LQ    +K   ++F  F   +  + K +      P  +L  L+    +P  E P+++ A+ +V +  T   +  + ++ D V +M G    DLN S   LI+  +G++KY  A+ +G P++   W+   WE +QN+  L    +  +++Y  P+     + +TG    ER+ ++++  + G  Y+  L +  CTHLI  + +G+K+ECA+KW +  V+  WL DS+ KG+  DE +Y +  RA       T + + N   ++
Sbjct:    9 FIVKFVESKGSNAEYAVKAYEAILELQSEKYLKKVKEEEVLQMEEKDKSL-FVFSSFTSPAFLHCKKHGCRIVSPLVVLYCLQQQRCVPKAEKPVYNMAMADVTISCTSLEKAARVDVMDLVQLMGGRACLDLNVSVTHLIAGEVGSKKYLVAANLGKPILLPSWVKACWEKSQNS--LFRYTDLPMDEYLCPVLLGCTVCVTGLSSTERKEVQRLCEQHGAKYSGQLKMNECTHLIVSEPAGQKYECARKWNVYCVSLHWLFDSIEKGFCQDESRYAVERRASNTSRPHTSTPTGNGRKED 307

HSP 2 Score: 83.9593 bits (206), Expect = 5.957e-16
Identity = 53/184 (28.80%), Postives = 88/184 (47.83%), Query Frame = 2 
Query: 2150 ENKSEKI---FVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGS------FQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLIN 2674
            E K EK    F + ++     +     ++ LGG +     ++   +H++ G P R EKYLAA+AAGKW+LH SY++     GR+++E  ++WG   +     L S+  +  +    LA AA R+     GS      F  W V L  ++ +     R+++SGG  ++P   P      TH   +
Sbjct: 1208 EEKKEKQPPRFQMSSLSPQERIDYSHVIEDLGGVVLEKQSFDPSCSHIIVGSPLRNEKYLAAMAAGKWILHRSYLEVCRGVGRFIQEDDFEWGS--SCILDALPSITLQQRR----LATAAMRWRKALQGSREREGAFSGWTVMLNIDQSRESGFRRLLQSGGAKVLPGPSPSLYREATHLFAD 1385
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Medaka
Match: ect2 (epithelial cell transforming 2 [Source:NCBI gene;Acc:101157762])

HSP 1 Score: 92.8189 bits (229), Expect = 9.555e-19
Identity = 68/220 (30.91%), Postives = 109/220 (49.55%), Query Frame = 3 
Query:  282 SIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGF-NEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            S  + + N+I+  GP  +      +E +     PL+ TA+ N+ +C TGF N+E  K L + V  M GT+ KD +     L++ S    KYR A  +G P+++  WI K WE   + D   G +E+  E +++P F +  +S  GF   E+  +E+  +K GG Y   L VG   CTHL+  + S  +   + K K+  V  +W   S++      E  Y
Sbjct:  140 SFLHRRDNRIV--GPPVVFHCAAKEEPMLFSCRPLYCTAMLNLSLCLTGFRNKEEMKNLVNLVHHMGGTIRKDFSTKVTHLVAYSTHGEKYRLAVCMGTPILTPLWIQKSWERRDDVDFHAGQEEFRSE-FKVPPFQDCVLSFLGFSEEEKANMEERTLKHGGRY---LEVGDERCTHLVVEENSVRELLTSSK-KLYVVKQEWFWGSIQMDARAGESMY 352
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Medaka
Match: ect2 (epithelial cell transforming 2 [Source:NCBI gene;Acc:101157762])

HSP 1 Score: 92.8189 bits (229), Expect = 1.207e-18
Identity = 68/220 (30.91%), Postives = 109/220 (49.55%), Query Frame = 3 
Query:  282 SIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGF-NEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            S  + + N+I+  GP  +      +E +     PL+ TA+ N+ +C TGF N+E  K L + V  M GT+ KD +     L++ S    KYR A  +G P+++  WI K WE   + D   G +E+  E +++P F +  +S  GF   E+  +E+  +K GG Y   L VG   CTHL+  + S  +   + K K+  V  +W   S++      E  Y
Sbjct:  140 SFLHRRDNRIV--GPPVVFHCAAKEEPMLFSCRPLYCTAMLNLSLCLTGFRNKEEMKNLVNLVHHMGGTIRKDFSTKVTHLVAYSTHGEKYRLAVCMGTPILTPLWIQKSWERRDDVDFHAGQEEFRSE-FKVPPFQDCVLSFLGFSEEEKANMEERTLKHGGRY---LEVGDERCTHLVVEENSVRELLTSSK-KLYVVKQEWFWGSIQMDARAGESMY 352
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Medaka
Match: ect2 (epithelial cell transforming 2 [Source:NCBI gene;Acc:101157762])

HSP 1 Score: 92.4337 bits (228), Expect = 1.283e-18
Identity = 68/220 (30.91%), Postives = 109/220 (49.55%), Query Frame = 3 
Query:  282 SIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGF-NEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            S  + + N+I+  GP  +      +E +     PL+ TA+ N+ +C TGF N+E  K L + V  M GT+ KD +     L++ S    KYR A  +G P+++  WI K WE   + D   G +E+  E +++P F +  +S  GF   E+  +E+  +K GG Y   L VG   CTHL+  + S  +   + K K+  V  +W   S++      E  Y
Sbjct:  109 SFLHRRDNRIV--GPPVVFHCAAKEEPMLFSCRPLYCTAMLNLSLCLTGFRNKEEMKNLVNLVHHMGGTIRKDFSTKVTHLVAYSTHGEKYRLAVCMGTPILTPLWIQKSWERRDDVDFHAGQEEFRSE-FKVPPFQDCVLSFLGFSEEEKANMEERTLKHGGRY---LEVGDERCTHLVVEENSVRELLTSSK-KLYVVKQEWFWGSIQMDARAGESMY 321
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Medaka
Match: ect2 (epithelial cell transforming 2 [Source:NCBI gene;Acc:101157762])

HSP 1 Score: 92.4337 bits (228), Expect = 1.482e-18
Identity = 68/220 (30.91%), Postives = 109/220 (49.55%), Query Frame = 3 
Query:  282 SIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGF-NEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVG--ICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY 932
            S  + + N+I+  GP  +      +E +     PL+ TA+ N+ +C TGF N+E  K L + V  M GT+ KD +     L++ S    KYR A  +G P+++  WI K WE   + D   G +E+  E +++P F +  +S  GF   E+  +E+  +K GG Y   L VG   CTHL+  + S  +   + K K+  V  +W   S++      E  Y
Sbjct:  140 SFLHRRDNRIV--GPPVVFHCAAKEEPMLFSCRPLYCTAMLNLSLCLTGFRNKEEMKNLVNLVHHMGGTIRKDFSTKVTHLVAYSTHGEKYRLAVCMGTPILTPLWIQKSWERRDDVDFHAGQEEFRSE-FKVPPFQDCVLSFLGFSEEEKANMEERTLKHGGRY---LEVGDERCTHLVVEENSVRELLTSSK-KLYVVKQEWFWGSIQMDARAGESMY 352
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Planmine SMEST
Match: SMESG000012344.1 (SMESG000012344.1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.000e+0
Identity = 559/562 (99.47%), Postives = 560/562 (99.64%), Query Frame = 3 
Query:  102 MQIWYVYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAIEIPKSPIAVPISNPIPDLSINWDLMDQYNLGNVSNPFDXXXXXXXXXXXXXXXFGIFHKYRFFLHNLSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLLSIESDFMFTRMDINPNILTGTILSFSGYSVDEE 1787
            MQIWYVYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKS+KILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILEREIIEKIIIKCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWLQACLDGKALSDSKQF IEIPKSPIAVPISNPIPDLSINWDLMDQYNLGNVSNPFDSNSERINNENINPNSFGIFHKYRFFLHNLSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLLSIESDFMFTRMDINPNILTGTILSFSGYSVDE 
Sbjct:    1 MQIWYVYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSHKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILEREIIEKIIIKCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFDIEIPKSPIAVPISNPIPDLSINWDLMDQYNLGNVSNPFDSNSERINNENINPNSFGIFHKYRFFLHNLSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQLLSIESDFMFTRMDINPNILTGTILSFSGYSVDER 562

HSP 2 Score: 714.916 bits (1844), Expect = 0.000e+0
Identity = 361/364 (99.18%), Postives = 363/364 (99.73%), Query Frame = 2 
Query: 1784 RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDCIAHREMLSTDLYSLELLANSVDKGKTEINHGVRKRKASEEIDTRPISKRKICEGAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLIPFYLSARNDCERKKFMKKFTIPVTEMDKLFGKITERSRCARKSKIF 2875
            RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLIISKPDKKKFPSSKIWNIPAVTKHWIGDCIAHREMLSTDLYSLELLANSVDK KTEINHGVRKRKASEEIDTRPISKRKICEGAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRW++EYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLIPFYLSARNDCERKKFMKKFTIPVTEMDKLFGKITERSRCARKSKIF
Sbjct:  562 RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLIISKPDKKKFPSSKIWNIPAVTKHWIGDCIAHREMLSTDLYSLELLANSVDKEKTEINHGVRKRKASEEIDTRPISKRKICEGAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWIDEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLIPFYLSARNDCERKKFMKKFTIPVTEMDKLFGKITERSRCARKSKIF 925
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Planmine SMEST
Match: SMESG000021513.1 (SMESG000021513.1)

HSP 1 Score: 377.867 bits (969), Expect = 5.093e-122
Identity = 218/552 (39.49%), Postives = 326/552 (59.06%), Query Frame = 3 
Query:  255 YIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLN---------------------------TKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAIEIPKSPIAVPISNPIPDLSINWDLMDQYNLGNV-------SNPFDXXXXXXXXXXXXXXXFGIFHKYRFFLHNLSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLCRSPPQECSS---LVTVFYLTECVKHKQLLSIESDFMFTRMDINPN----ILTGTILSFSGYSVDEE 1787
            ++ GYFE  S+ YL+S K+  FGPQCLL Y   D+ LP    PLFS ALK++ VCF+   EE K +L++KV MMHG VS  L A+ +ILIS S+ +RKYR ASE GIPVV K+W+ ++W L++N   L+G D+  I++Y+IPI HNL+I L+GF   ER+  ++++ + GG Y P+L V  CTHLIA  ASG+KF  AKKW I TV  +WL++S++KGYACD  +YQLS   ++PG         ++  ++ KII D+K N                           ++IW DC  Q LDC+ EFV KW NLI E GG+   ++     +  THIIVGD +KL EK D I Y+  DWLQACL+   L +  Q+ ++     I  P +N   D   NW  M++ + G+        +N  +S     +  +I+    GIF+K+ F + N +  +   + + I  N+G +  + +G+++ +L + +  +P +  SS   +VTV+Y+ +C++  Q+L ++SDF+F  + I+ N    IL GT++SFSGY  +E 
Sbjct:    2 FVLGYFECSSLKYLESLKLPIFGPQCLLAYFGKDDVLPKSPYPLFSIALKDIHVCFSSIEEENKIDLTNKVKMMHGFVSDALKANVQILISSSVQSRKYRVASEKGIPVVRKEWVEELWTLSKNCVQLNGVDQILIDKYQIPILHNLKICLSGFNESERDEAKQMVNENGGSYYPNLNVNDCTHLIAKNASGQKFSYAKKWNITTVTCEWLIESIKKGYACDVDQYQLSDEIHVPG---------STPKKDSKIIMDSKCNEISVIFDGSHADETRLDNHDESDCVPDSRIWIDCHFQLLDCKEEFVTKWENLIKENGGKIVSSV----TMPPTHIIVGDCTKLTEKTDGILYLKSDWLQACLNENRLVNVHQYIVD-----IYSPAANVQSD---NWGSMNELDWGDTMKTVEVNNNKDESTVIEKSTMSIDAIPNGIFYKFIFHMDNETCEKSIRIVNSITENAGTVISTTEGSTHYILPN-IFSTPIKSISSSTRVVTVYYIEQCLEQNQILPLDSDFLFNPISIDSNHYNTILVGTVISFSGYPAEER 531

HSP 2 Score: 81.2629 bits (199), Expect = 5.093e-122
Identity = 51/65 (78.46%), Postives = 54/65 (83.08%), Query Frame = 2 
Query: 1784 RSLLKSICSAAGATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDCI 1978
            R LLK IC+  GATVQDQMSRK +P K I ATTHLIISKPD KKFPSSK W IPAVTKHWIG+CI
Sbjct:  531 RQLLKEICTKLGATVQDQMSRKKIPSKRILATTHLIISKPDNKKFPSSKSWGIPAVTKHWIGECI 595

HSP 3 Score: 257.684 bits (657), Expect = 5.093e-71
Identity = 121/259 (46.72%), Postives = 177/259 (68.34%), Query Frame = 2 
Query: 2060 NHGVRKRKASEEIDTRPISKRKIC----EGAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPLIEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLIPFYLSARNDCERKKFMKKFTIPVTEMDKL 2824
            +H     K   + + + ISK ++       +E+ +   EK+F+   I K       E ++ LGG++EL +D++   THL+ G P   EKYLAAIAAGKWVLH SY+D+S++ G+W+EE ++QWGG R +EF++L      + K  LAL DA+K +H+ GGG+F++WRVCL+E+ EK+EKM +IIE+GGGT+VP  EP+ +L VTHFL+NK+ CSGLPDL+ + CY+I++IP +LSA+ND E K+  KKF +   EM K 
Sbjct:  906 SHCENNDKVQWDYEQKSISKHELSGSFSPASEITQLDIEKVFMFSNIAKTLEQHYDEEIRNLGGSLELNSDFSMRMTHLICGEPCLNEKYLAAIAAGKWVLHKSYLDESISQGKWLEETEFQWGGSRKNEFLQLNPTK-TYGKIFLALLDASKHWHEIGGGAFKEWRVCLIEKNEKYEKMCKIIEAGGGTVVPFSEPISQLGVTHFLVNKSQCSGLPDLSQYPCYKIEMIPSFLSAQNDREIKRCNKKFIVSAAEMQKF 1163
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Planmine SMEST
Match: SMESG000035281.1 (SMESG000035281.1)

HSP 1 Score: 163.31 bits (412), Expect = 1.429e-40
Identity = 116/407 (28.50%), Postives = 200/407 (49.14%), Query Frame = 3 
Query:  105 QIWYVYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNK---HCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAI--EIP 1310
            ++W++YF+ D+ +P+  +AY++L  +E  ++  +K  +    S + K   +  ++F  F+ ++  YLKS +   FGP  +L  +  D +LP   +PLF+  LKN ++  +  +   K++L + + MM GT S+    S +IL++ ++ + KY +A    I V+S DWI  IW      DP    +E ++++++IPIF NL+I L+GF   ER  I+ II   GG +  +LT    THLI   +   K+  A +W +  V   WL DS++ GY  +  KY+                                 N  I++ C+I F+ C  EF+ +W  +I    G+   + N +     TH I+ +   L  K     YV+  WL+  ++       K+F +  EIP
Sbjct:    3 KVWFLYFINDSPSPMFDKAYDSL--LEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPF---NEQYLKRFKIPIFFNLKICLSGFLEPERNTIKNIINNNGGIHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYE--------------------------------FNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEK--TTHFIIDNKVGLITKNYG-NYVDKSWLEKSINNGKRLPLKEFLVLGEIP 369

HSP 2 Score: 125.561 bits (314), Expect = 7.537e-29
Identity = 74/209 (35.41%), Postives = 108/209 (51.67%), Query Frame = 2 
Query: 2135 GAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPL---IEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLIPFYLS 2752
            G  +    + ++F+L          L + + +LGG +      +  FTHL+  R  R +K+L+A+A+GKW++H+SYI+      RW+ E  Y+WG   T+  I     A       L LA  AKR+ + GG +F+DWR+ L+E   K E   RI  SGGGT++P+   + PV E  VTH  IN N C+  P L     Y    I F LS
Sbjct: 1107 GRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLI-----AANDRDDLLPLALCAKRWREFGGKAFKDWRIILIESESKIESFKRIFVSGGGTVLPIGSSLPPVNE--VTHSFINLN-CTNYPKLQLPFEYHWIKINFILS 1307

HSP 3 Score: 61.6178 bits (148), Expect = 7.852e-14
Identity = 72/265 (27.17%), Postives = 116/265 (43.77%), Query Frame = 3 
Query: 1032 LNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWL-QACLDGKALSDSKQFAIEIPKSPIAVPISNPIPDLSIN----WDLMDQYNLGNVSNPFDXXXXXXXXXXXXXXXFGIFHKYRFFL-HNLSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLC-RSPPQECSSLVTVFYLTECVKHKQLLSIESDFMF---TRMDINPNIL----TGTILSFSGYSVDE 1784
            + + ++++C+I F  C   F+ KW+ +I    G      ++ S+   THI+V D   L   +   TYVN  WL Q+   GK L          P     V  SN I DLSI+    W  + +  +   +   DSNS             G+F+    F+  ++S      +  +I   SG I  +P  +  IV    L  R      S LVT +Y+++C+ + ++L ++ DF+F       INP +L      ++L F  Y  DE
Sbjct:  599 IESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISE-SEFSITPPTHIVVDDDWALS-IDSRFTYVNKSWLEQSIHAGKCL----------PVDIFLVTDSNGI-DLSIDDQSPW--ISESTIQQETRIIDSNSTTD----------GLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDE 838

HSP 4 Score: 35.8094 bits (81), Expect = 7.852e-14
Identity = 16/59 (27.12%), Postives = 33/59 (55.93%), Query Frame = 2 
Query: 1817 GATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDCIAHREM 1993
            GATVQD + +    +K++ A+T ++    +     S   W+IP V + W+ +C+  +++
Sbjct:  847 GATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQL 905
[Close]
BLAST of DNA topoisomerase 2-binding protein 1 vs. Planmine SMEST
Match: SMESG000035281.1 (SMESG000035281.1)

HSP 1 Score: 162.925 bits (411), Expect = 2.240e-40
Identity = 116/406 (28.57%), Postives = 199/406 (49.01%), Query Frame = 3 
Query:  108 IWYVYFVEDTKTPLLVQAYNALDGMENANLSWIKTEDILQTSTFNK---HCSYIFGYFEEKSIYYLKSNKILAFGPQCLLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHGTVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTDPLDGADEYWIEQYRIPIFHNLQISLTGFPILERXXXXXXXXXCGGFYNPDLTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKYQLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWLQACLDGKALSDSKQFAI--EIP 1310
            +W++YF+ D+ +P+  +AY++L  +E  ++  +K  +    S + K   +  ++F  F+ ++  YLKS +   FGP  +L  +  D +LP   +PLF+  LKN ++  +  +   K++L + + MM GT S+    S +IL++ ++ + KY +A    I V+S DWI  IW      DP    +E ++++++IPIF NL+I L+GF   ER  I+ II   GG +  +LT    THLI   +   K+  A +W +  V   WL DS++ GY  +  KY+                                 N  I++ C+I F+ C  EF+ +W  +I    G+   + N +     TH I+ +   L  K     YV+  WL+  ++       K+F +  EIP
Sbjct:    5 VWFLYFINDSPSPMFDKAYDSL--LEFEDVKAVKLSENFIMSNYEKLESNSCFVFSEFKGEAFEYLKSLERTIFGPNIILYCVSQDLSLPKTRLPLFNRKLKNYKISSSQLDVMEKQKLINLIKMMEGTYSEKFTNSIQILLTRNVESPKYSSAHNNLITVLSPDWIYDIWNSQIENDPF---NEQYLKRFKIPIFFNLKICLSGFLEPERNTIKNIINNNGGIHFGELTCNEATHLITKISKSRKYSYALQWNVKIVCINWLHDSLKDGYCKEYNKYE--------------------------------FNPNIFEHCIISFIGCSDEFINQWKVIIENNNGKVSLDPNLIEK--TTHFIIDNKVGLITKNYG-NYVDKSWLEKSINNGKRLPLKEFLVLGEIP 370

HSP 2 Score: 125.561 bits (314), Expect = 6.983e-29
Identity = 74/209 (35.41%), Postives = 108/209 (51.67%), Query Frame = 2 
Query: 2135 GAELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLVSGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSEFIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKMSRIIESGGGTIVPL---IEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLIPFYLS 2752
            G  +    + ++F+L          L + + +LGG +      +  FTHL+  R  R +K+L+A+A+GKW++H+SYI+      RW+ E  Y+WG   T+  I     A       L LA  AKR+ + GG +F+DWR+ L+E   K E   RI  SGGGT++P+   + PV E  VTH  IN N C+  P L     Y    I F LS
Sbjct: 1108 GRPVSSTSAPRLFLLTGFQDRETTKLSDIIHRLGGEVLESNLLDRPFTHLICNRLQRNDKFLSAVASGKWIMHSSYIEACHRESRWLNETLYEWGSSGTNTLI-----AANDRDDLLPLALCAKRWREFGGKAFKDWRIILIESESKIESFKRIFVSGGGTVLPIGSSLPPVNE--VTHSFINLN-CTNYPKLQLPFEYHWIKINFILS 1308

HSP 3 Score: 61.6178 bits (148), Expect = 8.313e-14
Identity = 72/265 (27.17%), Postives = 116/265 (43.77%), Query Frame = 3 
Query: 1032 LNTKIWKDCVIQFLDCEPEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITYVNGDWL-QACLDGKALSDSKQFAIEIPKSPIAVPISNPIPDLSIN----WDLMDQYNLGNVSNPFDXXXXXXXXXXXXXXXFGIFHKYRFFL-HNLSRVQIKAVSDLIINNSGIINCSPDGASYIVLSDSLC-RSPPQECSSLVTVFYLTECVKHKQLLSIESDFMF---TRMDINPNIL----TGTILSFSGYSVDE 1784
            + + ++++C+I F  C   F+ KW+ +I    G      ++ S+   THI+V D   L   +   TYVN  WL Q+   GK L          P     V  SN I DLSI+    W  + +  +   +   DSNS             G+F+    F+  ++S      +  +I   SG I  +P  +  IV    L  R      S LVT +Y+++C+ + ++L ++ DF+F       INP +L      ++L F  Y  DE
Sbjct:  600 IESNMFENCLISFHGCSDGFIGKWTAIINSRSGRVISE-SEFSITPPTHIVVDDDWALS-IDSRFTYVNKSWLEQSIHAGKCL----------PVDIFLVTDSNGI-DLSIDDQSPW--ISESTIQQETRIIDSNSTTD----------GLFNGILVFIDQSISDYSSSRLVKIITEKSGEITENPSNSRLIVTGAFLAHRYKCGPESKLVTEYYISQCIANDRILDVDGDFLFYPTVVAGINPGLLLVGCVISLLGFDSYEFDE 839

HSP 4 Score: 35.8094 bits (81), Expect = 8.313e-14
Identity = 16/59 (27.12%), Postives = 33/59 (55.93%), Query Frame = 2 
Query: 1817 GATVQDQMSRKPLPDKNIFATTHLXXXXXXXXXXXXXXXWNIPAVTKHWIGDCIAHREM 1993
            GATVQD + +    +K++ A+T ++    +     S   W+IP V + W+ +C+  +++
Sbjct:  848 GATVQDIVMKNDCRNKSLKASTVVVAKDANSNGLKSPLDWHIPVVNRDWLLECVTRKQL 906
The following BLAST results are available for this feature:
BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TOPBP12.380e-3328.74DNA topoisomerase II binding protein 1 [Source:HGN... [more]
TOPBP16.278e-3328.40DNA topoisomerase II binding protein 1 [Source:HGN... [more]
ECT26.566e-1929.36epithelial cell transforming 2 [Source:HGNC Symbol... [more]
ECT26.859e-1929.36epithelial cell transforming 2 [Source:HGNC Symbol... [more]
ECT26.859e-1929.36epithelial cell transforming 2 [Source:HGNC Symbol... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mus-1011.894e-1924.65pep chromosome:WBcel235:I:10479283:10484969:-1 gen... [more]
ect-21.283e-822.18ECT2 (Mammalian Rho GEF) homolog [Source:UniProtK... [more]
ect-21.283e-822.18ECT2 (Mammalian Rho GEF) homolog [Source:UniProtK... [more]
ect-21.474e-822.18ECT2 (Mammalian Rho GEF) homolog [Source:UniProtK... [more]
ect-21.474e-822.18ECT2 (Mammalian Rho GEF) homolog [Source:UniProtK... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mus1014.164e-2326.23gene:FBgn0002878 transcript:FBtr0073817[more]
pbl8.924e-1325.56gene:FBgn0003041 transcript:FBtr0112817[more]
pbl9.288e-1325.56gene:FBgn0003041 transcript:FBtr0076771[more]
pbl6.858e-1227.78gene:FBgn0003041 transcript:FBtr0076772[more]
pbl2.269e-1125.98gene:FBgn0003041 transcript:FBtr0076773[more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
topbp15.041e-3327.90DNA topoisomerase II binding protein 1 [Source:ZFI... [more]
topbp15.621e-3327.90DNA topoisomerase II binding protein 1 [Source:ZFI... [more]
topbp15.621e-3327.90DNA topoisomerase II binding protein 1 [Source:ZFI... [more]
ect23.476e-1927.00epithelial cell transforming 2 [Source:ZFIN;Acc:ZD... [more]
ect23.975e-1927.00epithelial cell transforming 2 [Source:ZFIN;Acc:ZD... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ppp1r14b4.716e-3424.36protein phosphatase 1 regulatory inhibitor subunit... [more]
ppp1r14b2.098e-3127.51protein phosphatase 1 regulatory inhibitor subunit... [more]
ppp1r14b2.174e-3127.51protein phosphatase 1 regulatory inhibitor subunit... [more]
ppp1r14b2.886e-3127.51protein phosphatase 1 regulatory inhibitor subunit... [more]
ppp1r14b3.305e-3127.51protein phosphatase 1 regulatory inhibitor subunit... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Topbp19.045e-3229.33topoisomerase (DNA) II binding protein 1 [Source:M... [more]
Ect25.484e-1828.89ect2 oncogene [Source:MGI Symbol;Acc:MGI:95281][more]
Ect25.484e-1828.89ect2 oncogene [Source:MGI Symbol;Acc:MGI:95281][more]
Ect25.580e-1828.89ect2 oncogene [Source:MGI Symbol;Acc:MGI:95281][more]
Slf11.129e-1234.33SMC5-SMC6 complex localization factor 1 [Source:MG... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q800K6|TOB1A_XENLA8.749e-3329.00DNA topoisomerase 2-binding protein 1-A OS=Xenopus... [more]
sp|Q7ZZY3|TOB1B_XENLA2.853e-3227.07DNA topoisomerase 2-binding protein 1-B OS=Xenopus... [more]
sp|Q92547|TOPB1_HUMAN3.015e-3228.40DNA topoisomerase 2-binding protein 1 OS=Homo sapi... [more]
sp|Q6ZQF0|TOPB1_MOUSE6.327e-3129.33DNA topoisomerase 2-binding protein 1 OS=Mus muscu... [more]
sp|Q9H8V3|ECT2_HUMAN3.474e-1829.36Protein ECT2 OS=Homo sapiens OX=9606 GN=ECT2 PE=1 ... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A430Q6U01.946e-6626.25Topoisomerase (DNA) II binding protein 1 OS=Schist... [more]
A0A183KJ351.106e-6526.09Uncharacterized protein OS=Schistosoma curassoni O... [more]
A0A183NWR55.200e-6525.76Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A183LAC45.938e-6525.76Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A095AI886.087e-6525.84DNA topoisomerase 2-binding protein 1 OS=Schistoso... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
topbp12.861e-3328.99DNA topoisomerase II binding protein 1 [Source:NCB... [more]
ect21.204e-2129.87epithelial cell transforming 2 [Source:ZFIN;Acc:ZD... [more]
slf18.104e-828.06SMC5-SMC6 complex localization factor 1 [Source:ZF... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
topbp11.563e-3123.94DNA topoisomerase II binding protein 1 [Source:ZFI... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO389466.539e-3633.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO418641.780e-1125.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO445603.391e-635.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
topbp12.543e-3427.48DNA topoisomerase II binding protein 1 [Source:NCB... [more]
ect29.555e-1930.91epithelial cell transforming 2 [Source:NCBI gene;A... [more]
ect21.207e-1830.91epithelial cell transforming 2 [Source:NCBI gene;A... [more]
ect21.283e-1830.91epithelial cell transforming 2 [Source:NCBI gene;A... [more]
ect21.482e-1830.91epithelial cell transforming 2 [Source:NCBI gene;A... [more]
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BLAST of DNA topoisomerase 2-binding protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000012344.10.000e+099.47SMESG000012344.1[more]
SMESG000021513.15.093e-12239.49SMESG000021513.1[more]
SMESG000035281.11.429e-4028.50SMESG000035281.1[more]
SMESG000035281.12.240e-4028.57SMESG000035281.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017131 ID=SMED30017131|Name=DNA topoisomerase 2-binding protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=3003bp
TCTGAACTCTTATTGTAAAATTTGTTCAAATAATGTTTTTTGTTCGGATA
ATACTTTTTTTCAAATTCTTTCTTCTAATAAAATTTGATTTTAAGAAAAT
AATGCAAATATGGTATGTCTATTTCGTTGAAGATACAAAAACTCCATTAT
TAGTCCAAGCATATAACGCTCTAGATGGAATGGAGAATGCTAATTTAAGC
TGGATTAAAACAGAAGATATTCTACAAACTTCAACTTTTAATAAACATTG
TTCATATATTTTCGGATACTTTGAAGAAAAATCAATATATTACTTAAAAT
CTAATAAAATTTTGGCTTTTGGGCCTCAATGTTTATTGGATTACTTGCGA
AATGATGAAACTTTACCAATATGTGAAATTCCATTATTTAGTACAGCCTT
AAAAAATGTAGAAGTTTGTTTTACTGGTTTCAATGAAGAAGTCAAAAAAG
AATTAAGTGATAAAGTTGTGATGATGCATGGAACGGTTAGCAAAGATTTA
AATGCTTCTGCGAAAATTCTTATATCTCCGTCTATTGGAACTCGCAAATA
TCGAGCAGCATCAGAAATTGGTATTCCTGTCGTTTCAAAAGACTGGATTA
ACAAAATTTGGGAATTAACTCAAAATACAGATCCTCTAGATGGCGCGGAT
GAGTACTGGATTGAACAATATCGAATCCCAATCTTTCACAATTTACAAAT
CAGTTTAACCGGATTTCCGATATTAGAACGAGAAATAATAGAAAAGATTA
TAATAAAATGTGGTGGATTTTATAACCCAGATTTGACAGTTGGGATTTGT
ACTCATTTGATAGCAATGCAGGCTTCCGGTGAGAAATTCGAGTGTGCCAA
AAAATGGAAAATTATAACAGTCAATTACCAATGGTTGCTGGACTCTGTTA
GAAAAGGATACGCTTGCGATGAAAAGAAATATCAATTATCCGGTAGAGCC
TATATTCCAGGCTTATCTCAAACTGATTCATCCAGCAACAATTCAGCTGG
CCAAAATGATAAAATCATTTGCGACAATAAATTAAACACGAAAATATGGA
AAGATTGTGTGATTCAATTCTTAGATTGCGAACCGGAATTTGTTGAAAAA
TGGTCAAATTTAATCACTGAATTTGGAGGCGAGGCATGTAAAAACTTAAA
CGATTTGTCAGTGGTCTTTGTAACTCACATTATTGTAGGAGATGCCAGCA
AATTGAAGGAAAAGGAAGATGATATTACCTATGTAAACGGTGATTGGCTG
CAAGCCTGTTTAGATGGGAAGGCGTTATCAGATTCTAAACAATTTGCTAT
TGAAATTCCCAAATCACCGATAGCCGTTCCGATCAGTAACCCGATACCGG
ATTTATCAATAAATTGGGATTTAATGGACCAATATAATCTGGGAAATGTT
TCCAATCCGTTTGACAGCAATTCCGAGCGTATCAATAACGAAAATATAAA
TCCAAACTCTTTTGGTATATTTCATAAATATCGATTTTTCCTCCATAATT
TGTCTCGGGTCCAAATAAAAGCTGTTTCCGATTTAATTATCAACAATTCA
GGCATAATCAATTGCTCTCCAGACGGTGCAAGTTATATTGTATTATCGGA
TAGCTTGTGTCGGTCACCGCCACAGGAATGTAGTAGTTTGGTTACGGTTT
TCTATCTCACCGAATGTGTCAAACATAAACAGTTGCTGTCAATTGAATCT
GATTTCATGTTTACTCGAATGGATATCAATCCCAATATTCTCACTGGGAC
CATTCTCTCATTTAGTGGATATTCAGTCGATGAAGAAGTCTTTTGAAATC
GATTTGTTCCGCTGCTGGAGCGACCGTTCAAGATCAAATGTCACGGAAAC
CGCTTCCTGATAAAAACATTTTTGCTACGACACATCTGATAATTTCAAAA
CCGGATAAAAAGAAATTTCCATCGAGTAAGATCTGGAACATTCCAGCTGT
TACCAAGCACTGGATAGGGGATTGTATTGCACACAGAGAAATGCTTTCCA
CTGACTTATACTCCCTGGAACTCTTGGCCAATTCAGTAGACAAAGGAAAG
ACTGAAATCAATCACGGAGTTAGGAAAAGAAAAGCCAGCGAAGAAATTGA
TACTAGACCAATCAGCAAGCGAAAAATCTGTGAGGGTGCGGAATTGATTG
AAAATAAATCGGAAAAGATATTTGTGTTATGCACAATAGATAAGGCGAGT
TATGTAACGTTACGGGAAGCGGTTAAGAAATTGGGAGGAGCAATGGAACT
GGGAGCGGATTACAATGGAGAATTCACTCATTTAGTGTCTGGAAGGCCGG
GCCGAACTGAGAAATATCTCGCGGCTATTGCAGCCGGCAAATGGGTGTTG
CACACATCATACATTGATGATTCTATGGCTGCTGGCCGCTGGATGGAGGA
GTATCAATATCAATGGGGAGGACTGAGAACATCTGAGTTTATTGAGTTGG
AATCGGTGGCTTGCAGATTTACAAAATTTGATTTAGCCTTGGCTGATGCT
GCTAAGCGATTCCATGACAACGGTGGTGGTTCGTTTCAAGATTGGCGAGT
GTGCTTGGTGGAAGAAAGGGAAAAGTTTGAAAAAATGTCGAGAATTATCG
AATCTGGAGGTGGAACTATTGTGCCCTTAATTGAACCAGTGAAAGAATTG
CACGTTACACATTTTCTAATAAATAAAAATCTATGTTCCGGTTTGCCTGA
TTTGACCAGCCATTCTTGTTACGAGATTGACTTGATTCCGTTTTACTTAT
CTGCTCGGAATGATTGTGAAAGGAAAAAGTTTATGAAAAAGTTCACTATT
CCAGTTACGGAGATGGATAAATTATTTGGAAAGATCACTGAGAGATCAAG
ATGTGCCAGAAAGTCAAAAATATTTTAATAACTTTTTTTATTTCCAATAG
ATTATTTTTTGTAAATAAATTTTTATATCCTATTTGATTTTTGTAGTGTT
TCTATTAGAAATAATATATTTCATGTTAATAGTATGCAAATTCTTCATGT
GAC
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protein sequence of SMED30017131-orf-1

>SMED30017131-orf-1 ID=SMED30017131-orf-1|Name=SMED30017131-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=347bp
MSRKPLPDKNIFATTHLIISKPDKKKFPSSKIWNIPAVTKHWIGDCIAHR
EMLSTDLYSLELLANSVDKGKTEINHGVRKRKASEEIDTRPISKRKICEG
AELIENKSEKIFVLCTIDKASYVTLREAVKKLGGAMELGADYNGEFTHLV
SGRPGRTEKYLAAIAAGKWVLHTSYIDDSMAAGRWMEEYQYQWGGLRTSE
FIELESVACRFTKFDLALADAAKRFHDNGGGSFQDWRVCLVEEREKFEKM
SRIIESGGGTIVPLIEPVKELHVTHFLINKNLCSGLPDLTSHSCYEIDLI
PFYLSARNDCERKKFMKKFTIPVTEMDKLFGKITERSRCARKSKIF*
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protein sequence of SMED30017131-orf-2

>SMED30017131-orf-2 ID=SMED30017131-orf-2|Name=SMED30017131-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=588bp
MFFVRIILFFKFFLLIKFDFKKIMQIWYVYFVEDTKTPLLVQAYNALDGM
ENANLSWIKTEDILQTSTFNKHCSYIFGYFEEKSIYYLKSNKILAFGPQC
LLDYLRNDETLPICEIPLFSTALKNVEVCFTGFNEEVKKELSDKVVMMHG
TVSKDLNASAKILISPSIGTRKYRAASEIGIPVVSKDWINKIWELTQNTD
PLDGADEYWIEQYRIPIFHNLQISLTGFPILEREIIEKIIIKCGGFYNPD
LTVGICTHLIAMQASGEKFECAKKWKIITVNYQWLLDSVRKGYACDEKKY
QLSGRAYIPGLSQTDSSSNNSAGQNDKIICDNKLNTKIWKDCVIQFLDCE
PEFVEKWSNLITEFGGEACKNLNDLSVVFVTHIIVGDASKLKEKEDDITY
VNGDWLQACLDGKALSDSKQFAIEIPKSPIAVPISNPIPDLSINWDLMDQ
YNLGNVSNPFDSNSERINNENINPNSFGIFHKYRFFLHNLSRVQIKAVSD
LIINNSGIINCSPDGASYIVLSDSLCRSPPQECSSLVTVFYLTECVKHKQ
LLSIESDFMFTRMDINPNILTGTILSFSGYSVDEEVF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR036420BRCT_dom_sf
IPR001357BRCT_dom
Vocabulary: molecular function
TermDefinition
GO:0016853isomerase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001357BRCT domainSMARTSM00292BRCT_7coord: 215..292
e-value: 3.3E-16
score: 69.9
coord: 120..196
e-value: 7.8E-10
score: 48.7
coord: 336..413
e-value: 0.0016
score: 27.7
coord: 477..549
e-value: 35.0
score: 7.3
IPR001357BRCT domainPFAMPF16589BRCT_2coord: 478..556
e-value: 5.8E-7
score: 29.8
IPR001357BRCT domainPFAMPF12738PTCB-BRCTcoord: 127..188
e-value: 6.4E-10
score: 38.8
coord: 222..284
e-value: 1.2E-13
score: 50.7
IPR001357BRCT domainPROSITEPS50172BRCTcoord: 359..423
score: 10.085
IPR001357BRCT domainPROSITEPS50172BRCTcoord: 213..302
score: 16.205
IPR001357BRCT domainPROSITEPS50172BRCTcoord: 118..189
score: 12.088
IPR036420BRCT domain superfamilyGENE3DG3DSA:3.40.50.10190coord: 216..306
e-value: 1.0E-21
score: 79.2
IPR036420BRCT domain superfamilyGENE3DG3DSA:3.40.50.10190coord: 119..215
e-value: 1.6E-18
score: 68.7
IPR036420BRCT domain superfamilyGENE3DG3DSA:3.40.50.10190coord: 20..118
e-value: 2.9E-8
score: 35.6
IPR036420BRCT domain superfamilyGENE3DG3DSA:3.40.50.10190coord: 470..562
e-value: 3.2E-9
score: 38.8
IPR036420BRCT domain superfamilyGENE3DG3DSA:3.40.50.10190coord: 323..426
e-value: 8.3E-10
score: 40.5
IPR036420BRCT domain superfamilySUPERFAMILYSSF52113BRCT domaincoord: 81..195
IPR036420BRCT domain superfamilySUPERFAMILYSSF52113BRCT domaincoord: 215..303
IPR036420BRCT domain superfamilySUPERFAMILYSSF52113BRCT domaincoord: 334..413
NoneNo IPR availablePANTHERPTHR13561DNA REPLICATION REGULATOR DPB11-RELATEDcoord: 80..588
NoneNo IPR availableCDDcd17731BRCT_TopBP1_rpt2_likecoord: 216..292
e-value: 3.55672E-19
score: 79.8914
NoneNo IPR availableCDDcd00027BRCTcoord: 342..409
e-value: 6.1131E-5
score: 39.2673
NoneNo IPR availableCDDcd17733BRCT_Ect2_rpt1coord: 123..194
e-value: 5.3709E-16
score: 70.8174
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.617