SMED30017099

Overview
NameSMED30017099
Smed IDSMED30017099
Length (bp)387
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30017099 (SMED30017099) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017099 (SMED30017099) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017099 (SMED30017099) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017099 (SMED30017099) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30017099

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30017099SMESG000030373.1 SmedASXL_018797SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30017099SMESG000030373.1 dd_Smed_v4_595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30017099SMESG000030373.1 dd_Smed_v4_595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30017099SMESG000030373.1 dd_Smed_v4_595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30017099SMESG000030373.1 dd_Smed_v4_595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30017099SMESG000030373.1 dd_Smed_v4_595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30017099SMESG000030373.1 dd_Smed_v4_595_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
Smed sexual biotypeSMED30017099SMESG000030373.1 Contig1380newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017099SMESG000030373.1 Contig1380uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017099SMESG000075440.1 Contig1380newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017099SMESG000075440.1 Contig1380uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30017099SMESG000030373.1 dd_Smed_v6_595_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30017099SMESG000030373.1 OX_Smed_1.0.21100ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
protonephridiaSMED30017099SMESG000030373.1 dd_Smed_v6_595_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30017099SMESG000030373.1 dd_Smed_v6_595_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30017099 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006026:19362..19907 -1850
Homology
[Close]
BLAST of SMED30017099 vs. Ensembl Human
Match: HSBP1 (heat shock factor binding protein 1 [Source:HGNC Symbol;Acc:HGNC:5203])

HSP 1 Score: 50.8322 bits (120), Expect = 4.592e-9
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. Ensembl Human
Match: HSBP1 (heat shock factor binding protein 1 [Source:HGNC Symbol;Acc:HGNC:5203])

HSP 1 Score: 50.8322 bits (120), Expect = 4.592e-9
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. Ensembl Fly
Match: CG5446 (gene:FBgn0032429 transcript:FBtr0333094)

HSP 1 Score: 46.9802 bits (110), Expect = 1.108e-7
Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRID 287
            ADP+  QEL  Y++ ++Q +QD    +S++I   +D +G  ++DLE+++ DLM Q+ I+
Sbjct:   22 ADPKNMQELTIYVQNLLQNVQDKFQTMSDQIITRIDDMGNRIDDLEKSIADLMNQAGIE 80
[Close]
BLAST of SMED30017099 vs. Ensembl Fly
Match: CG5446 (gene:FBgn0032429 transcript:FBtr0080388)

HSP 1 Score: 46.9802 bits (110), Expect = 1.108e-7
Identity = 22/59 (37.29%), Postives = 40/59 (67.80%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRID 287
            ADP+  QEL  Y++ ++Q +QD    +S++I   +D +G  ++DLE+++ DLM Q+ I+
Sbjct:   22 ADPKNMQELTIYVQNLLQNVQDKFQTMSDQIITRIDDMGNRIDDLEKSIADLMNQAGIE 80
[Close]
BLAST of SMED30017099 vs. Ensembl Zebrafish
Match: hsbp1b (heat shock factor binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1721])

HSP 1 Score: 49.2914 bits (116), Expect = 1.534e-8
Identity = 21/60 (35.00%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP++ Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKSVQDLTAVVQTLLQQMQDKFQTMSDQIIGRIDEMSTRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. Ensembl Zebrafish
Match: hsbp1a (heat shock factor binding protein 1a [Source:ZFIN;Acc:ZDB-GENE-050320-136])

HSP 1 Score: 48.9062 bits (115), Expect = 1.986e-8
Identity = 21/59 (35.59%), Postives = 40/59 (67.80%), Query Frame = 3 
Query:  114 DPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
            DP++ Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    5 DPKSSQDLTVVVQTLLQQMQDKFQTMSDQIIGRIDEMSTRIDDLEKNISDLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. Ensembl Zebrafish
Match: hsbp1a (heat shock factor binding protein 1a [Source:ZFIN;Acc:ZDB-GENE-050320-136])

HSP 1 Score: 48.9062 bits (115), Expect = 1.986e-8
Identity = 21/59 (35.59%), Postives = 40/59 (67.80%), Query Frame = 3 
Query:  114 DPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
            DP++ Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    5 DPKSSQDLTVVVQTLLQQMQDKFQTMSDQIIGRIDEMSTRIDDLEKNISDLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. Ensembl Zebrafish
Match: hsbp1l1 (heat shock factor binding protein 1 like 1 [Source:NCBI gene;Acc:799177])

HSP 1 Score: 45.8246 bits (107), Expect = 3.390e-7
Identity = 21/56 (37.50%), Postives = 36/56 (64.29%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQS 278
            ++ +  ++L   +E  MQQ+Q     +SE+I + MD +G  ++DLE+NV DLM Q+
Sbjct:    4 SESKAAKDLTAIMETTMQQLQSRFQNLSEQIISKMDEMGTRIDDLEKNVADLMTQA 59
[Close]
BLAST of SMED30017099 vs. Ensembl Xenopus
Match: hsbp1 (heat shock factor binding protein 1 [Source:NCBI gene;Acc:496903])

HSP 1 Score: 50.447 bits (119), Expect = 6.597e-9
Identity = 23/60 (38.33%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ +DE
Sbjct:    4 TDPKTVQDLTVVVQNLLQQMQDKFQTMSDQIIGRIDDMSTRIDDLEKNIADLMTQAGVDE 63
[Close]
BLAST of SMED30017099 vs. Ensembl Mouse
Match: Hsbp1 (heat shock factor binding protein 1 [Source:MGI Symbol;Acc:MGI:1915446])

HSP 1 Score: 49.2914 bits (116), Expect = 1.728e-8
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q++   +E ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTMQDITLVVETLLQQMQDKFQIMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. Ensembl Mouse
Match: Hsbp1 (heat shock factor binding protein 1 [Source:MGI Symbol;Acc:MGI:1915446])

HSP 1 Score: 49.2914 bits (116), Expect = 1.728e-8
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q++   +E ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTMQDITLVVETLLQQMQDKFQIMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. UniProt/SwissProt
Match: sp|O75506|HSBP1_HUMAN (Heat shock factor-binding protein 1 OS=Homo sapiens OX=9606 GN=HSBP1 PE=1 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.205e-8
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. UniProt/SwissProt
Match: sp|Q3ZC22|HSBP1_BOVIN (Heat shock factor-binding protein 1 OS=Bos taurus OX=9913 GN=HSBP1 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.205e-8
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. UniProt/SwissProt
Match: sp|Q5RDI2|HSBP1_PONAB (Heat shock factor-binding protein 1 OS=Pongo abelii OX=9601 GN=HSBP1 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.428e-8
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. UniProt/SwissProt
Match: sp|Q8K3X8|HSBP1_RAT (Heat shock factor-binding protein 1 OS=Rattus norvegicus OX=10116 GN=Hsbp1 PE=3 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 1.041e-7
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q++   +E ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTMQDITLVVETLLQQMQDKFQIMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. UniProt/SwissProt
Match: sp|Q9CQZ1|HSBP1_MOUSE (Heat shock factor-binding protein 1 OS=Mus musculus OX=10090 GN=Hsbp1 PE=1 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 1.209e-7
Identity = 22/60 (36.67%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP+T Q++   +E ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKTMQDITLVVETLLQQMQDKFQIMSDQIIGRIDDMSSRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. TrEMBL
Match: A0A5K4F7U5 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 4.040e-13
Identity = 31/68 (45.59%), Postives = 45/68 (66.18%), Query Frame = 3 
Query:   87 ARDSKFGIADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             RD  FGI DP T  EL++Y++ I QQMQD + + SEKI   ++ +G  ++ LE+NV DLM Q+ + E
Sbjct:    8 VRDRSFGIPDPHTVTELSNYMQNIFQQMQDTLQQTSEKITTRIEEMGSKIDSLEKNVADLMTQAGVHE 75
[Close]
BLAST of SMED30017099 vs. TrEMBL
Match: C1LJV1 (Putative heat shock factor binding protein 1 OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 8.447e-13
Identity = 30/68 (44.12%), Postives = 45/68 (66.18%), Query Frame = 3 
Query:   87 ARDSKFGIADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             +D  FGI DP T  EL++Y++ I QQMQD + + SEKI   ++ +G  ++ LE+NV DLM Q+ + E
Sbjct:    8 VKDKSFGIPDPHTVNELSNYMQNIFQQMQDTLQQTSEKITTRIEEMGSKIDSLEKNVADLMTQAGVHE 75
[Close]
BLAST of SMED30017099 vs. TrEMBL
Match: A0A267GXM6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004952g1 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.457e-12
Identity = 29/68 (42.65%), Postives = 48/68 (70.59%), Query Frame = 3 
Query:   84 YARDSKFGIADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRID 287
            YA+D  FGIADP +  EL ++++ ++Q + DN +  S+KI   +D +GR +++LERNV DLM Q+ ++
Sbjct:   53 YAKDKSFGIADPGSVNELTNHLQSLLQGLSDNFNHTSDKITGRIDEMGRRIDELERNVADLMTQAGVE 120
[Close]
BLAST of SMED30017099 vs. TrEMBL
Match: A0A1I8IZ87 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.772e-12
Identity = 29/68 (42.65%), Postives = 48/68 (70.59%), Query Frame = 3 
Query:   84 YARDSKFGIADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRID 287
            YA+D  FGIADP +  EL ++++ ++Q + DN +  S+KI   +D +GR +++LERNV DLM Q+ ++
Sbjct:   59 YAKDKSFGIADPGSVNELTNHLQSLLQGLSDNFNHTSDKITGRIDEMGRRIDELERNVADLMTQAGVE 126
[Close]
BLAST of SMED30017099 vs. TrEMBL
Match: A0A1I8HFY0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.914e-12
Identity = 29/68 (42.65%), Postives = 48/68 (70.59%), Query Frame = 3 
Query:   84 YARDSKFGIADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRID 287
            YA+D  FGIADP +  EL ++++ ++Q + DN +  S+KI   +D +GR +++LERNV DLM Q+ ++
Sbjct:   60 YAKDKSFGIADPGSVNELTNHLQSLLQGLSDNFNHTSDKITGRIDEMGRRIDELERNVADLMTQAGVE 127
[Close]
BLAST of SMED30017099 vs. Ensembl Cavefish
Match: hsbp1a (heat shock factor-binding protein 1-like [Source:NCBI gene;Acc:103023435])

HSP 1 Score: 47.3654 bits (111), Expect = 6.886e-8
Identity = 20/60 (33.33%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             +P++ Q+L   ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TEPKSAQDLTVVVQTLLQQMQDKFQTMSDQIIGRIDEMSTRIDDLEKNISDLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. Ensembl Cavefish
Match: hsbp1b (heat shock factor binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1721])

HSP 1 Score: 44.669 bits (104), Expect = 6.561e-7
Identity = 21/60 (35.00%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP++ Q+L   ++ ++QQMQD    +S++I    +I+   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKSVQDLTGVVQTLLQQMQDKFQTMSDQIIGR-NILFTRIDDLEKNIADLMTQAGVEE 62
[Close]
BLAST of SMED30017099 vs. Ensembl Nematostella
Match: EDO35978 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJY7])

HSP 1 Score: 50.0618 bits (118), Expect = 1.731e-9
Identity = 21/62 (33.87%), Postives = 42/62 (67.74%), Query Frame = 3 
Query:  108 IADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDEP 293
            + +P+  Q+L  +++ ++ QMQD    +S++I   +D +   ++DLE+N+G+LM Q+ +DEP
Sbjct:   11 VENPENLQDLTGFVQTLLTQMQDKFQNMSDQIITRIDEMSNRIDDLEKNIGELMNQAGVDEP 72
[Close]
BLAST of SMED30017099 vs. Ensembl Medaka
Match: hsbp1a (heat shock factor binding protein 1 [Source:NCBI gene;Acc:101175153])

HSP 1 Score: 49.2914 bits (116), Expect = 1.165e-8
Identity = 21/60 (35.00%), Postives = 41/60 (68.33%), Query Frame = 3 
Query:  111 ADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNMDIIGRGVNDLERNVGDLMAQSRIDE 290
             DP++ Q+L + ++ ++QQMQD    +S++I   +D +   ++DLE+N+ DLM Q+ ++E
Sbjct:    4 TDPKSVQDLTNVVQTLLQQMQDKFQTMSDQIIGRIDEMSTRIDDLEKNIADLMTQAGVEE 63
[Close]
BLAST of SMED30017099 vs. Planmine SMEST
Match: SMESG000030373.1 (SMESG000030373.1)

HSP 1 Score: 108.997 bits (271), Expect = 2.265e-32
Identity = 52/61 (85.25%), Postives = 56/61 (91.80%), Query Frame = 3 
Query:   63 MSTCRDSYARDSKFGIADPQTPQELAHYIERIMQQMQDNVDRVSEKINNN--MDIIGRGVN 239
            MSTCRDSYARDSKFGIADPQTPQELAHYIERIMQQMQDNVDRVSEKINNN  + ++ R +N
Sbjct:    1 MSTCRDSYARDSKFGIADPQTPQELAHYIERIMQQMQDNVDRVSEKINNNNILLLVSRNLN 61
The following BLAST results are available for this feature:
BLAST of SMED30017099 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
HSBP14.592e-936.67heat shock factor binding protein 1 [Source:HGNC S... [more]
HSBP14.592e-936.67heat shock factor binding protein 1 [Source:HGNC S... [more]
back to top
BLAST of SMED30017099 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30017099 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG54461.108e-737.29gene:FBgn0032429 transcript:FBtr0333094[more]
CG54461.108e-737.29gene:FBgn0032429 transcript:FBtr0080388[more]
back to top
BLAST of SMED30017099 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
hsbp1b1.534e-835.00heat shock factor binding protein 1b [Source:ZFIN;... [more]
hsbp1a1.986e-835.59heat shock factor binding protein 1a [Source:ZFIN;... [more]
hsbp1a1.986e-835.59heat shock factor binding protein 1a [Source:ZFIN;... [more]
hsbp1l13.390e-737.50heat shock factor binding protein 1 like 1 [Source... [more]
back to top
BLAST of SMED30017099 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hsbp16.597e-938.33heat shock factor binding protein 1 [Source:NCBI g... [more]
back to top
BLAST of SMED30017099 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Hsbp11.728e-836.67heat shock factor binding protein 1 [Source:MGI Sy... [more]
Hsbp11.728e-836.67heat shock factor binding protein 1 [Source:MGI Sy... [more]
back to top
BLAST of SMED30017099 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O75506|HSBP1_HUMAN2.205e-836.67Heat shock factor-binding protein 1 OS=Homo sapien... [more]
sp|Q3ZC22|HSBP1_BOVIN2.205e-836.67Heat shock factor-binding protein 1 OS=Bos taurus ... [more]
sp|Q5RDI2|HSBP1_PONAB2.428e-836.67Heat shock factor-binding protein 1 OS=Pongo abeli... [more]
sp|Q8K3X8|HSBP1_RAT1.041e-736.67Heat shock factor-binding protein 1 OS=Rattus norv... [more]
sp|Q9CQZ1|HSBP1_MOUSE1.209e-736.67Heat shock factor-binding protein 1 OS=Mus musculu... [more]
back to top
BLAST of SMED30017099 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K4F7U54.040e-1345.59Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
C1LJV18.447e-1344.12Putative heat shock factor binding protein 1 OS=Sc... [more]
A0A267GXM61.457e-1242.65Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8IZ871.772e-1242.65Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HFY01.914e-1242.65Uncharacterized protein OS=Macrostomum lignano OX=... [more]
back to top
BLAST of SMED30017099 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hsbp1a6.886e-833.33heat shock factor-binding protein 1-like [Source:N... [more]
hsbp1b6.561e-735.00heat shock factor binding protein 1b [Source:ZFIN;... [more]
back to top
BLAST of SMED30017099 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30017099 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30017099 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO359781.731e-933.87Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMED30017099 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hsbp1a1.165e-835.00heat shock factor binding protein 1 [Source:NCBI g... [more]
back to top
BLAST of SMED30017099 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000030373.12.265e-3285.25SMESG000030373.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017099 ID=SMED30017099|Name=SMED30017099|organism=Schmidtea mediterranea sexual|type=transcript|length=387bp
CTTTGATTTGAAATTTGTTTAAATTGTTGTTGTTACAGTAACATCTAGTA
AATACTTTCTAAATGTCAACTTGTCGAGATTCATATGCTAGAGATTCTAA
GTTTGGAATTGCTGATCCACAGACTCCACAAGAATTAGCTCATTATATTG
AGAGAATAATGCAGCAAATGCAAGACAATGTTGACCGTGTTTCCGAGAAA
ATCAACAATAATATGGATATAATCGGCCGTGGAGTAAACGATTTGGAAAG
AAATGTTGGGGATCTAATGGCTCAATCAAGGATTGACGAGCCTTGAGAAT
TTTGTATTTGTTAACAGTTTTATTTCAAACTAATTTATTTGATATAAAAC
AATATTTTTCTCAAATATAATTCAGTAAATTTTCAAT
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0000418head
PLANA:0000464GABAergic neuron
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR009643HS1-bd
Vocabulary: molecular function
TermDefinition
GO:0003714transcription corepressor activity
Vocabulary: biological process
TermDefinition
GO:1903507negative regulation of nucleic acid-templated transcription
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.5.430coord: 19..66
e-value: 3.0E-11
score: 45.1
NoneNo IPR availablePANTHERPTHR19424:SF3HEAT SHOCK FACTOR-BINDING PROTEIN 1coord: 17..76
IPR009643Heat shock factor binding 1PFAMPF06825HSBP1coord: 23..72
e-value: 5.3E-13
score: 48.6
IPR009643Heat shock factor binding 1PANTHERPTHR19424HEAT SHOCK FACTOR BINDING PROTEIN 1coord: 17..76