SMED30017058

Overview
NameSMED30017058
Smed IDSMED30017058
Length (bp)1950
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30017058 (SMED30017058) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017058 (SMED30017058) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017058 (SMED30017058) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017058 (SMED30017058) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017058

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30017058SMESG000051187.1 SmedASXL_080578SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30017058SMESG000051187.1 SmedASXL_080578SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30017058 vs. Planmine SMEST
Match: SMESG000054252.1 (SMESG000054252.1)

HSP 1 Score: 136.346 bits (342), Expect = 2.311e-42
Identity = 65/89 (73.03%), Postives = 71/89 (79.78%), Query Frame = 3 
Query: 1602 GTQTRDLEDVDRTTMTVPTRFAGRYRQTLWDRGYTISTYGTPEWPSRNNNGSETDVELPSILIKREPGRNPAREIRQKTEVELHRFEQL 1868
            GT + + +DVD TT+TVPT FAGRY Q LW+ GYTISTYGTP+WP R  NG E D ELPSILIKREPGRNP REIRQK E ELHR EQL
Sbjct:   13 GTHSGNWKDVDLTTLTVPTLFAGRYTQALWNLGYTISTYGTPKWPYRKTNGPEGDFELPSILIKREPGRNPTREIRQKAEGELHRLEQL 101

HSP 2 Score: 56.225 bits (134), Expect = 2.311e-42
Identity = 25/28 (89.29%), Postives = 26/28 (92.86%), Query Frame = 1 
Query: 1867 YRFLAKRQREQLARINRDIASGRIPSSD 1950
            YRFL KRQ+E LARINRDIASGRIPSSD
Sbjct:  105 YRFLVKRQKEHLARINRDIASGRIPSSD 132
[Close]
BLAST of SMED30017058 vs. Planmine SMEST
Match: SMESG000054252.1 (SMESG000054252.1)

HSP 1 Score: 135.961 bits (341), Expect = 5.461e-42
Identity = 65/89 (73.03%), Postives = 71/89 (79.78%), Query Frame = 3 
Query: 1602 GTQTRDLEDVDRTTMTVPTRFAGRYRQTLWDRGYTISTYGTPEWPSRNNNGSETDVELPSILIKREPGRNPAREIRQKTEVELHRFEQL 1868
            GT + + +DVD TT+TVPT FAGRY Q LW+ GYTISTYGTP+WP R  NG E D ELPSILIKREPGRNP REIRQK E ELHR EQL
Sbjct:   13 GTHSGNWKDVDLTTLTVPTLFAGRYTQALWNLGYTISTYGTPKWPYRKTNGPEGDFELPSILIKREPGRNPTREIRQKAEGELHRLEQL 101

HSP 2 Score: 55.4546 bits (132), Expect = 5.461e-42
Identity = 25/28 (89.29%), Postives = 26/28 (92.86%), Query Frame = 1 
Query: 1867 YRFLAKRQREQLARINRDIASGRIPSSD 1950
            YRFL KRQ+E LARINRDIASGRIPSSD
Sbjct:  105 YRFLVKRQKEHLARINRDIASGRIPSSD 132
[Close]
BLAST of SMED30017058 vs. Planmine SMEST
Match: SMESG000008335.1 (SMESG000008335.1)

HSP 1 Score: 131.339 bits (329), Expect = 1.367e-36
Identity = 58/81 (71.60%), Postives = 72/81 (88.89%), Query Frame = 3 
Query: 1626 DVDRTTMTVPTRFAGRYRQTLWDRGYTISTYGTPEWPSRNNNGSETDVELPSILIKREPGRNPAREIRQKTEVELHRFEQL 1868
            D++ TT+TVP+RFAGRYRQ LWD+GYTIST+GTPEWP+R +N  + D ELPS++IKREPGRNPAREIRQ+TE  L+R+EQL
Sbjct:   95 DMESTTLTVPSRFAGRYRQALWDQGYTISTHGTPEWPNRRSNNCDEDTELPSLVIKREPGRNPAREIRQETEGLLYRYEQL 175

HSP 2 Score: 41.9726 bits (97), Expect = 1.367e-36
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 1 
Query: 1867 YRFLAKRQREQLARINRDIASGRIPSSD 1950
            YR+LA ++R +LARIN DI +G IPSSD
Sbjct:  179 YRYLANQKRRRLARINEDITNGHIPSSD 206
[Close]
BLAST of SMED30017058 vs. Planmine SMEST
Match: SMESG000060519.1 (SMESG000060519.1)

HSP 1 Score: 127.102 bits (318), Expect = 9.251e-34
Identity = 58/81 (71.60%), Postives = 63/81 (77.78%), Query Frame = 3 
Query: 1626 DVDRTTMTVPTRFAGRYRQTLWDRGYTISTYGTPEWPSRNNNGSETDVELPSILIKREPGRNPAREIRQKTEVELHRFEQL 1868
            DV+RTT+TV  RFAGRY Q LWD GY+ISTYGT EWP RN    + D ELP ILIKREPGRNPAREIRQK E  LH FE+L
Sbjct:  857 DVERTTLTVQNRFAGRYHQALWDPGYSISTYGTLEWPCRNPESRDKDTELPCILIKREPGRNPAREIRQKAEGALHHFEKL 937

HSP 2 Score: 36.5798 bits (83), Expect = 9.251e-34
Identity = 18/28 (64.29%), Postives = 20/28 (71.43%), Query Frame = 1 
Query: 1867 YRFLAKRQREQLARINRDIASGRIPSSD 1950
            YR L  +QRE LARI  DIAS RIP+ D
Sbjct:  941 YRPLTNQQREHLARIIMDIASWRIPNLD 968
[Close]
BLAST of SMED30017058 vs. Planmine SMEST
Match: SMESG000057450.1 (SMESG000057450.1)

HSP 1 Score: 102.834 bits (255), Expect = 2.821e-32
Identity = 48/70 (68.57%), Postives = 54/70 (77.14%), Query Frame = 3 
Query: 1659 RFAGRYRQTLWDRGYTISTYGTPEWPSRNNNGSETDVELPSILIKREPGRNPAREIRQKTEVELHRFEQL 1868
            +   R RQ LWDRGYTISTYGTPEWP R  +    D+ELPS+LIKRE G+NPAREIRQ+ E ELHR EQL
Sbjct:   45 QVCSRCRQALWDRGYTISTYGTPEWPKRKTDELRDDIELPSVLIKRELGQNPAREIRQEAERELHRLEQL 114

HSP 2 Score: 46.2098 bits (108), Expect = 2.821e-32
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 1 
Query: 1867 YRFLAKRQREQLARINRDIASGRIPSSD 1950
            YRFLA+RQ+E LAR+NRDI SG IPS D
Sbjct:  118 YRFLARRQKEYLARVNRDIRSGHIPSLD 145

HSP 3 Score: 30.0314 bits (66), Expect = 2.821e-32
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 2 
Query: 1619 FGRCRPYHDDGTDKI 1663
             GRCRPYH DG D++
Sbjct:   32 LGRCRPYHSDGADQV 46
The following BLAST results are available for this feature:
BLAST of SMED30017058 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017058 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000054252.12.311e-4273.03SMESG000054252.1[more]
SMESG000054252.15.461e-4273.03SMESG000054252.1[more]
SMESG000008335.11.367e-3671.60SMESG000008335.1[more]
SMESG000060519.19.251e-3471.60SMESG000060519.1[more]
SMESG000057450.12.821e-3268.57SMESG000057450.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017058 ID=SMED30017058|Name=SMED30017058|organism=Schmidtea mediterranea sexual|type=transcript|length=1950bp
AGATTAACAAAAGAAAATCATGTGAGCCACAAATGTGTACAGATGGGAGA
CCGAGAATCCCGCGTAGTATTCAACGATCTCTGGAGGATGTTTGAACAAA
CGATTCGATGAGGCCTAATATTTCCGAAGATTGTTTCCCGGACAACGTGG
AACCTATAATAATGCACAAGTGGGAGTAAAATGGATACCGAACAATGGCA
ATTGAACTAATTATGGCGGATCAGCCCACGTTTAGCATTAATATACCAAA
ATCTTCGAAAGAAATAAAATTGATTAAGAATTTTCCGACTCTTATTTAAG
TCGACAGAACAATGGGTAGAAACTCTACCGCTGGTGACTGGAGTGTGAAA
TGTAATATTACACACCCAGCAAGTTGACAAAAATAATTCACCGAAGAAGG
AAAACGTATGACGTAGCCAGTAGATAGAAATGTAGACAGAAAATCATCTG
CTATCTAGCGATCGATAGTAGGAGTGGGGAAGAGACGAACCACTCATAAT
TGGCAAGGAACGTCGACGTTGCCACGTGGCGGTACACACCTACAGAGCCC
ACGAGTATAAATGTCCAGCGAAATTGTATACGTTGTAATTACTGAAATAT
TAAAATGACAGCAGCTTCAGCGAATGAACCCATGAAAATGAAAGAAAATT
CTCTAATTAATGATTCAAATCAACGAGCACCGGAGAAGTATAACCATCGA
ACCTATGGAGTTTCGGATCACGGACGAAACCACATTGCTGCGCGGAGTTC
TATGGTAGGAAAGGCTATCCAAGTCATGTTTTTGTGTCAATAAAAATTTA
TGTCATAAATTGCTGTTAATTCTTTTTTGTACAATTGAAAATGGCGATTC
AGTAATTCCGAAAACTCCTAGGCATAAATTGCTTCAACTACTAGATTTAT
CCAATAATTAAAACTACCACTGAGCCATCAGGAACTTCTGTAGCCACGTC
GATAAACAAATCGTGAACCACATTTTGTCAGAGTAAGATTTCGAGGATAA
GAGCGAGCCGTAAAGTAGTGTCGAGAGTTCGCGTCGAAATAATTCCAAAT
GTATAATGGCATTCTCCAAGGTACATGAATCTTCTAGAGTCTGAAATTCC
CAAGGTCTCCGTAATTATCCCAGTTAATTTACCTGGTTGACCTTAACTGA
AATAGTGCTCAAATCCTCCATTGAAGGTAAAAGTGATCACCACTCAAGGT
GATCCATTTTTAGCTTGTGATTAGGAGCCGGAGGGAATGGAATATTACAT
TTAGGGCTTTCGATACACGGAAACGGTCAGCTCCATGCTGTTTTACTATA
AGCGAAAGGAAAAAGTTAGTGAAAATGTTATTAGTACTACCATAATGAGA
GAATCAAATTTAAGACCACTATTATAGGATATTCTAGATCATTTCCCACC
TAAGCACAATTTTAGAAGGGGGTATCTCTAACTTACATGGATGTTGTAGT
CAAACCTGAACGATTATAGTTTTGCTTATTCAAACAAATTGGATTTATAT
GTCCCTGAAAGTTAATTTAGAAAATTGGTTATAAATGTATATCGATCATT
GATAGGAAGGAAATTAATAAGGACTAATAATCTTTTAATGTCGTTTTGAC
AGGCACTCAGACCCGTGATTTGGAAGATGTAGACCGTACCACGATGACGG
TACCGACAAGATTCGCCGGAAGGTATCGTCAGACCTTATGGGACAGAGGC
TACACAATATCGACGTATGGGACCCCCGAGTGGCCGAGTAGGAACAACAA
TGGATCGGAAACCGATGTAGAGCTCCCTAGCATACTCATCAAGAGGGAGC
CGGGCAGAAACCCAGCCCGAGAGATCCGGCAGAAGACGGAAGTCGAACTG
CACAGATTTGAGCAGTTACCGATTTCTCGCGAAGCGGCAAAGAGAACAAC
TTGCAAGGATCAACCGGGATATCGCGAGCGGGCGTATCCCTAGTTCAGAC
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protein sequence of SMED30017058-orf-1

>SMED30017058-orf-1 ID=SMED30017058-orf-1|Name=SMED30017058-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MTVPTRFAGRYRQTLWDRGYTISTYGTPEWPSRNNNGSETDVELPSILIK
REPGRNPAREIRQKTEVELHRFEQLPISREAAKRTTCKDQPGYRERAYP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell