SMED30017054

Overview
NameSMED30017054
Smed IDSMED30017054
Length (bp)1654
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30017054 (SMED30017054) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017054 (SMED30017054) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017054 (SMED30017054) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017054 (SMED30017054) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017054

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 14

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30017054h1SMcG0022351 Contig50528newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017054h1SMcG0022351 Contig50528uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017054h1SMcG0022355 Contig50528newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017054h1SMcG0022355 Contig50528uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017054h1SMcG0022353 Contig50528newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017054h1SMcG0022353 Contig50528uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30017054h1SMcG0022355 dd_Smed_v4_3962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30017054h1SMcG0022355 dd_Smed_v4_3962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30017054h1SMcG0022355 dd_Smed_v4_3962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30017054h1SMcG0022353 dd_Smed_v4_3962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30017054h1SMcG0022353 dd_Smed_v4_3962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30017054h1SMcG0022353 dd_Smed_v4_3962_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30017054h1SMcG0022355 dd_Smed_v6_3962_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30017054h1SMcG0022353 dd_Smed_v6_3962_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30017054 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002574:38022..43733 -7549
v31.002574:31859..35146 +7302
v31.002574:6839..8924 +6534
Homology
[Close]
BLAST of SMED30017054 vs. Planmine SMEST
Match: SMESG000023216.1 (SMESG000023216.1)

HSP 1 Score: 934.48 bits (2414), Expect = 0.000e+0
Identity = 458/491 (93.28%), Postives = 470/491 (95.72%), Query Frame = 3 
Query:  135 MIECPLYSSIFKCWSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLHSGNQNGTIALYYTASRTRYTTHCTIEPINHLTVLISLGSEEIILNCKNAINLTSSVIDENDLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDQIELPLYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRNGTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNGNMNATGPGYVMESFMSQSNWILIGGFSTVMAALFVLYCIFRKQMKRAFKEMGKDRTQPRHYEVVENIYTISPPIRESCEYGMIEETEYAFIK 1607
            MIECPLYSSIFKCWSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLH GNQNGTIALYYTASRTRYTT+CTIEPINHLTVLISLGS  IILNCKNA+NL SSVIDE DLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCD IELP+YKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLR GTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRN NMNA GP YV ES  S    ILIG FSTVMAALFVLYCIF+K+MKRAFKE+GK+RTQPR+YE+VENI TIS PIRESC+YG IE++EYAFIK
Sbjct:    1 MIECPLYSSIFKCWSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLHPGNQNGTIALYYTASRTRYTTNCTIEPINHLTVLISLGSGIIILNCKNAMNLISSVIDEKDLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDPIELPVYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRYGTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNRNMNAAGPSYVPES--SPFYLILIGFFSTVMAALFVLYCIFKKKMKRAFKELGKNRTQPRNYELVENICTISSPIRESCDYGKIEDSEYAFIK 489
[Close]
BLAST of SMED30017054 vs. Planmine SMEST
Match: SMESG000023216.1 (SMESG000023216.1)

HSP 1 Score: 929.087 bits (2400), Expect = 0.000e+0
Identity = 458/491 (93.28%), Postives = 470/491 (95.72%), Query Frame = 3 
Query:  135 MIECPLYSSIFKCWSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLHSGNQNGTIALYYTASRTRYTTHCTIEPINHLTVLISLGSEEIILNCKNAINLTSSVIDENDLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDQIELPLYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRNGTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNGNMNATGPGYVMESFMSQSNWILIGGFSTVMAALFVLYCIFRKQMKRAFKEMGKDRTQPRHYEVVENIYTISPPIRESCEYGMIEETEYAFIK 1607
            MIECPLYSSIFKCWSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLH GNQNGTIALYYTASRTRYTT+CTIEPINHLTVLISLGS  IILNCKNA+NL SSVIDEN LHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCD IELP+YKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLR GTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRN NMNA GP YV ES  S    ILIG FSTVMAALFVLYCIF+K+MKRAFKE+GK+RTQPR+YE+VENI TIS PIRESC+YG IE++EYAFIK
Sbjct:    1 MIECPLYSSIFKCWSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLHPGNQNGTIALYYTASRTRYTTNCTIEPINHLTVLISLGSGIIILNCKNAMNLISSVIDEN-LHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDPIELPVYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRYGTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNRNMNAAGPSYVPES--SPFYLILIGFFSTVMAALFVLYCIFKKKMKRAFKELGKNRTQPRNYELVENICTISSPIRESCDYGKIEDSEYAFIK 488
[Close]
BLAST of SMED30017054 vs. Planmine SMEST
Match: SMESG000023223.1 (SMESG000023223.1)

HSP 1 Score: 823.928 bits (2127), Expect = 0.000e+0
Identity = 403/446 (90.36%), Postives = 413/446 (92.60%), Query Frame = 3 
Query:  135 MIECPLYSSIFKCWSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLHSGNQNGTIALYYTASRTRYTTHCTIEPINHLTVLISLGSEEIILNCKNAINLTSSVIDENDLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDQIELPLYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRNGTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNGNMNATGPGYVMESFMSQSNWILIGGFSTVMAAL-FVLYCIFRKQMKR 1469
            MIECPLYSSI KCWSVLAAIILFSPL STSDYIYHELNGNLTYSKWNLSLFTKNF GILLYATNANESLIIKLHSGNQNGTIALYYTASRTRYTTHCT EPINHLTVLISLGS E+ILNCKNA+NLTSSVIDENDLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGIN NRCDQIELP+YKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLR GTITFTVNNINAI MVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRN NMNA GP YV ES +       +       +++ F +YCI  +  KR
Sbjct:    1 MIECPLYSSILKCWSVLAAIILFSPLSSTSDYIYHELNGNLTYSKWNLSLFTKNFGGILLYATNANESLIIKLHSGNQNGTIALYYTASRTRYTTHCTTEPINHLTVLISLGSGEMILNCKNAMNLTSSVIDENDLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINVNRCDQIELPVYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRYGTITFTVNNINAICMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNRNMNAAGPSYVPESNIHNILIYSLIIIYLSTSSIAFAIYCIKTRAKKR 446
[Close]
BLAST of SMED30017054 vs. Planmine SMEST
Match: SMESG000023217.1 (SMESG000023217.1)

HSP 1 Score: 551.592 bits (1420), Expect = 0.000e+0
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 3 
Query:  816 MYDCSLPGINANRCDQIELPLYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRNGTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNGNMNATGPGYVMESFMSQSNWILIGGFSTVMAALFVLYCIFRKQMKRAFKEMGKDRTQPRHYEVVENIYTISPPIRESCEYGMIEETEYAFIK 1607
            MYDCSLPGINANRCDQIELPLYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRNGTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNGNMNATGPGYVMESFMSQSNWILIGGFSTVMAALFVLYCIFRKQMKRAFKEMGKDRTQPRHYEVVENIYTISPPIRESCEYGMIEETEYAFIK
Sbjct:    1 MYDCSLPGINANRCDQIELPLYKQSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRNGTITFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNGNMNATGPGYVMESFMSQSNWILIGGFSTVMAALFVLYCIFRKQMKRAFKEMGKDRTQPRHYEVVENIYTISPPIRESCEYGMIEETEYAFIK 264
[Close]
BLAST of SMED30017054 vs. Planmine SMEST
Match: SMESG000023221.1 (SMESG000023221.1)

HSP 1 Score: 198.364 bits (503), Expect = 6.334e-57
Identity = 142/476 (29.83%), Postives = 232/476 (48.74%), Query Frame = 3 
Query:  219 TSDYIYHELNGNLTYSKWNLSLFTKNFEGILLYATNANESLIIKLHSGNQNG----TIALYYTASRTRYTTHCTIEPINHLTVLISLGSEEIILNCKNA--------INLTSSVIDENDLHKFPVPKDYYKAPNKTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHIFPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDQIELPLYKQSNQSYHRYGNY---NYNKTIRISLVWKQLYSKDFCLMEIKLRNGTI------TFTVNNINAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGIRIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSD-FTRSRNGNMNATGPGYVMESFMSQSNWILIGGFSTVMAALFVLYCIFRKQMKRAFKEMGKDRTQPRHYEVVENIYTISPPIRESCEYGMI 1580
            +S YIY EL  +L  S WN+SL  +  +GI+LYA ++   L+IK    N +     T  ++   S T Y      E   HL V+IS    + I N             N  S +  +N     P+PK + K   KTFI+MGNIGPY GYN K++YP K  L GC+EYFY N +P++++  G     SC   ++T    N+      ++ + M+DCSLP +N   C QIE+ +    NQS  +  +    N+  +I I+  WKQ+YS+++CL+EI    G +       F   ++   +    + + S +P     +++ L+ IN L I F   + ++K+ +G +I   I   +  PI I+VG  +    C  F G  G  Y+ S+ F      N +AT      ++  + S ++LIGG    ++ L +   I  K+    +  +  +R +  +   + ++  IS P+  S +YG +
Sbjct:   18 SSGYIYQELTESLVKSVWNISLHVRETKGIVLYAIDSQAQLLIKFTEENNDSRIFITFNIFSKPSLTIYAWSIKHELKTHLHVIIS--KRKDIFNITGPQYMQRLTDWNQISLLNFKNMFLSMPIPKGFLKYSKKTFIFMGNIGPYDGYN-KTYYPSKSHLNGCLEYFYSNLRPVSMQQNGAKFLKSCEVNSITNYICNKGGYLMTNLPIHMFDCSLPILNEKYCTQIEMLVSTADNQSLIKRSSIPPTNHRSSI-INFAWKQIYSRNYCLLEIDFNGGQVIQVAVDMFNYASVRRFNNENNNNNESPTPTIIKTNKLLLNDINNLFIEFSKKQIIIKISDGHQINTIIGSLLAHPITINVGNPQKRNNCERFVGAFGITYNLSEWFMHYTAYNQSATSIVRDSDNRNNLSLFLLIGGLLVWVSILLITMIITYKKYFSIY-SLINNRHRSGNGLYIRSVRDISRPVSNSDDYGTV 488
The following BLAST results are available for this feature:
BLAST of SMED30017054 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30017054 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000023216.10.000e+093.28SMESG000023216.1[more]
SMESG000023216.10.000e+093.28SMESG000023216.1[more]
SMESG000023223.10.000e+090.36SMESG000023223.1[more]
SMESG000023217.10.000e+0100.00SMESG000023217.1[more]
SMESG000023221.16.334e-5729.83SMESG000023221.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017054 ID=SMED30017054|Name=SMED30017054|organism=Schmidtea mediterranea sexual|type=transcript|length=1654bp
AATAAATACCGATCAAAGTTCGTTTTTAATTTTGAACATGAAATTCTAAA
AATATAAATGATGGATACCCGGTGTGTAAAACTGGATTTAGTTGACGATA
TGTTCGCTAATAATCTCACAGTTTGCGCCTTGTTATGATTGAATGTCCTC
TATATTCTTCTATCTTTAAGTGTTGGAGCGTCCTAGCAGCAATCATCCTA
TTCTCGCCTCTGCCCAGCACCTCTGATTACATTTATCACGAATTGAATGG
CAACCTAACATATTCAAAATGGAATCTTTCTTTATTCACCAAAAATTTCG
AAGGAATACTTTTATACGCCACCAATGCGAATGAAAGTCTTATAATTAAA
TTACATTCAGGTAATCAAAATGGAACAATTGCTTTATATTACACGGCCTC
TAGAACCAGGTACACTACCCACTGCACGATTGAACCGATCAATCATTTGA
CGGTTTTGATCAGTCTCGGATCCGAAGAAATTATCCTCAATTGCAAAAAC
GCAATAAACTTGACTTCTTCTGTCATTGATGAAAATGATCTCCATAAATT
CCCGGTACCGAAAGATTATTACAAAGCCCCGAACAAAACATTCATCTGGA
TGGGAAATATTGGACCGTATCTCGGTTACAATAATAAATCATTTTATCCA
TTAAAACAACAATTGATTGGTTGTGTTGAATATTTCTATTTCAATAATCA
ACCAATCGCATTGAAGAATCAAGGAAATCACATTTTCCCATCATGCACCA
GTCAGACCGTGACGGAAGAAACCGAAAATCGGACAGCGCGATCATCCAGA
TCAATAAATGTGCAAATGTATGATTGTTCCTTGCCAGGAATCAATGCCAA
CCGTTGCGATCAAATCGAATTGCCCCTCTATAAACAATCCAACCAATCAT
ATCACAGATATGGCAATTATAATTATAATAAAACAATAAGGATATCTTTA
GTTTGGAAGCAACTTTATTCGAAAGACTTTTGTTTGATGGAGATCAAATT
GAGAAATGGCACCATCACATTTACTGTCAATAATATTAATGCTATTTCCA
TGGTCATGTACAGTACTGATGCCAGTACTAGTCCGCGTTTCCAATTATTG
GATAGAATTTCATTGGATAAAATAAATAAATTATCCATTGCATTCGGAAT
CTCAGAATTTATGGTCAAGGTATTTAACGGGATCCGGATTTATAAATTTA
TTACGGATTCAGTTGGCGCACCAATCAAAATCGATGTCGGAAAATCCCGT
AATGAAAAAGTTTGCAGAACTTTTGAAGGAATTCTGGGTTTCCAATACGA
TTCCTCAGATTTCACGAGATCTCGGAACGGAAATATGAACGCTACCGGCC
CCGGCTATGTTATGGAGTCTTTTATGTCTCAATCAAATTGGATTCTGATC
GGAGGTTTCTCAACGGTCATGGCAGCATTATTCGTCTTATATTGCATTTT
TAGAAAGCAAATGAAAAGAGCGTTCAAAGAAATGGGTAAAGACCGAACTC
AACCAAGGCATTACGAAGTTGTAGAAAATATATACACGATTTCTCCTCCA
ATTAGAGAAAGCTGCGAATATGGGATGATTGAAGAAACAGAATATGCGTT
TATTAAATGATTCAAATTCAAAATATATTTACTCGTTTTAAATTTAAAAA
AAAA
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protein sequence of SMED30017054-orf-1

>SMED30017054-orf-1 ID=SMED30017054-orf-1|Name=SMED30017054-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=492bp
MIECPLYSSIFKCWSVLAAIILFSPLPSTSDYIYHELNGNLTYSKWNLSL
FTKNFEGILLYATNANESLIIKLHSGNQNGTIALYYTASRTRYTTHCTIE
PINHLTVLISLGSEEIILNCKNAINLTSSVIDENDLHKFPVPKDYYKAPN
KTFIWMGNIGPYLGYNNKSFYPLKQQLIGCVEYFYFNNQPIALKNQGNHI
FPSCTSQTVTEETENRTARSSRSINVQMYDCSLPGINANRCDQIELPLYK
QSNQSYHRYGNYNYNKTIRISLVWKQLYSKDFCLMEIKLRNGTITFTVNN
INAISMVMYSTDASTSPRFQLLDRISLDKINKLSIAFGISEFMVKVFNGI
RIYKFITDSVGAPIKIDVGKSRNEKVCRTFEGILGFQYDSSDFTRSRNGN
MNATGPGYVMESFMSQSNWILIGGFSTVMAALFVLYCIFRKQMKRAFKEM
GKDRTQPRHYEVVENIYTISPPIRESCEYGMIEETEYAFIK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013320ConA-like_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..29
score: 0.623
NoneNo IPR availableTMHMMTMhelixcoord: 417..439
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILYSSF49899Concanavalin A-like lectins/glucanasescoord: 30..195