SMED30017018

Overview


Name	SMED30017018
Smed ID	SMED30017018
Length (bp)	1008

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30017018 (SMED30017018) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30017018 (SMED30017018) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30017018 (SMED30017018) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30017018 (SMED30017018) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30017018

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30017018	SMESG000007294.1	dd_Smed_v4_371_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30017018	SMESG000007294.1	dd_Smed_v4_371_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30017018	SMESG000007294.1	dd_Smed_v4_371_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30017018	SMESG000007294.1	dd_Smed_v4_371_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30017018	SMESG000007294.1	dd_Smed_v4_371_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30017018	SMESG000007294.1	dd_Smed_v4_371_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30017018	SMESG000007294.1	dd_Smed_v4_371_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30017018	SMESG000007294.1	dd_Smed_v4_371_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30017018	SMESG000007294.1	dd_Smed_v6_371_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30017018 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.001677:56288..57438 -	4922

Homology

[Close]

BLAST of SMED30017018 vs. Ensembl Human
Match: NACA (nascent polypeptide associated complex subunit alpha [Source:HGNC Symbol;Acc:HGNC:7629])

HSP 1 Score: 165.622 bits (418), Expect = 2.645e-49
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 214

[Close]

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 214

[Close]

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 214

[Close]

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. Ensembl Human
Match: NACA (nascent polypeptide associated complex subunit alpha [Source:HGNC Symbol;Acc:HGNC:7629])

HSP 1 Score: 165.622 bits (418), Expect = 4.661e-49
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   81 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 234

[Close]

BLAST of SMED30017018 vs. Ensembl Celegans
Match: icd-2 (Nascent polypeptide-associated complex subunit alpha [Source:UniProtKB/Swiss-Prot;Acc:Q86S66])

HSP 1 Score: 152.525 bits (384), Expect = 7.309e-45
Identity = 92/163 (56.44%), Postives = 115/163 (70.55%), Query Frame = -3

Query:  353 GDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 841
            GDH D   ++KQSRSEKKARK  SKLGLK +TGV+RV IRKSKNILFV+ +P+V+KSP SDTY++FGEAKIED++  AQ++A E          +      AP    VE  + E     ED+TG+ E DI+L++ QA  +RNKAIRALK ADND+VNAIM LT
Sbjct:   46 GDHID--KQAKQSRSEKKARKLFSKLGLKQVTGVSRVCIRKSKNILFVINKPDVFKSPGSDTYIIFGEAKIEDLTQHAQMSAIE----------NLKPTREAPQLKTVEEDENEDVEFKEDSTGIEEKDIELVISQANTTRNKAIRALKEADNDIVNAIMSLT 196

[Close]

BLAST of SMED30017018 vs. Ensembl Celegans
Match: icd-2 (Nascent polypeptide-associated complex subunit alpha [Source:UniProtKB/Swiss-Prot;Acc:Q86S66])

HSP 1 Score: 147.517 bits (371), Expect = 3.432e-43
Identity = 88/163 (53.99%), Postives = 118/163 (72.39%), Query Frame = -3

Query:  353 GDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 841
            GDH D   ++KQSRSEKKARK  SKLGLK +TGV+RV IRKSKNILFV+ +P+V+KSP SDTY++FGEAKIED++  AQ++A E +  ++                +    ++E++  +ED+TG+ E DI+L++ QA  +RNKAIRALK ADND+VNAIM LT
Sbjct:   31 GDHID--KQAKQSRSEKKARKLFSKLGLKQVTGVSRVCIRKSKNILFVINKPDVFKSPGSDTYIIFGEAKIEDLTQHAQMSAIENLKPTREAP------------QLKTVEEDENEDVEEDSTGIEEKDIELVISQANTTRNKAIRALKEADNDIVNAIMSLT 179

[Close]

BLAST of SMED30017018 vs. Ensembl Celegans
Match: icd-2 (Nascent polypeptide-associated complex subunit alpha [Source:UniProtKB/Swiss-Prot;Acc:Q86S66])

HSP 1 Score: 147.902 bits (372), Expect = 4.272e-43
Identity = 88/163 (53.99%), Postives = 118/163 (72.39%), Query Frame = -3

Query:  353 GDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 841
            GDH D   ++KQSRSEKKARK  SKLGLK +TGV+RV IRKSKNILFV+ +P+V+KSP SDTY++FGEAKIED++  AQ++A E +  ++                +    ++E++  +ED+TG+ E DI+L++ QA  +RNKAIRALK ADND+VNAIM LT
Sbjct:   46 GDHID--KQAKQSRSEKKARKLFSKLGLKQVTGVSRVCIRKSKNILFVINKPDVFKSPGSDTYIIFGEAKIEDLTQHAQMSAIENLKPTREA------------PQLKTVEEDENEDVEEDSTGIEEKDIELVISQANTTRNKAIRALKEADNDIVNAIMSLT 194

[Close]

BLAST of SMED30017018 vs. Ensembl Fly
Match: Nacalpha (gene:FBgn0086904 transcript:FBtr0087858)

HSP 1 Score: 148.288 bits (373), Expect = 6.196e-43
Identity = 98/166 (59.04%), Postives = 120/166 (72.29%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            GATG   D +SK+KQSR EKKARK M KLGLK I GVNRV IRKSKNILFV+  P+VYK+P SDTY+VFGEAKIED+S QAQ+AAAEK    +A     +  +    AP+ E  +E+      D TG+ E DI+L++ QA  +R KAI+ALKN +ND+VNAIMELT
Sbjct:   55 GATGLPIDLVSKAKQSRGEKKARKIMLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAGAADSVGATTSVAPIAEEDEEDV-----DDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELT 215

[Close]

BLAST of SMED30017018 vs. Ensembl Fly
Match: Nacalpha (gene:FBgn0086904 transcript:FBtr0087859)

HSP 1 Score: 148.288 bits (373), Expect = 6.196e-43
Identity = 98/166 (59.04%), Postives = 120/166 (72.29%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            GATG   D +SK+KQSR EKKARK M KLGLK I GVNRV IRKSKNILFV+  P+VYK+P SDTY+VFGEAKIED+S QAQ+AAAEK    +A     +  +    AP+ E  +E+      D TG+ E DI+L++ QA  +R KAI+ALKN +ND+VNAIMELT
Sbjct:   55 GATGLPIDLVSKAKQSRGEKKARKIMLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAGAADSVGATTSVAPIAEEDEEDV-----DDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELT 215

[Close]

BLAST of SMED30017018 vs. Ensembl Fly
Match: Nacalpha (gene:FBgn0086904 transcript:FBtr0345195)

HSP 1 Score: 148.288 bits (373), Expect = 6.196e-43
Identity = 98/166 (59.04%), Postives = 120/166 (72.29%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            GATG   D +SK+KQSR EKKARK M KLGLK I GVNRV IRKSKNILFV+  P+VYK+P SDTY+VFGEAKIED+S QAQ+AAAEK    +A     +  +    AP+ E  +E+      D TG+ E DI+L++ QA  +R KAI+ALKN +ND+VNAIMELT
Sbjct:   55 GATGLPIDLVSKAKQSRGEKKARKIMLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAGAADSVGATTSVAPIAEEDEEDV-----DDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELT 215

[Close]

BLAST of SMED30017018 vs. Ensembl Fly
Match: Nacalpha (gene:FBgn0086904 transcript:FBtr0087860)

HSP 1 Score: 148.288 bits (373), Expect = 6.196e-43
Identity = 98/166 (59.04%), Postives = 120/166 (72.29%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            GATG   D +SK+KQSR EKKARK M KLGLK I GVNRV IRKSKNILFV+  P+VYK+P SDTY+VFGEAKIED+S QAQ+AAAEK    +A     +  +    AP+ E  +E+      D TG+ E DI+L++ QA  +R KAI+ALKN +ND+VNAIMELT
Sbjct:   55 GATGLPIDLVSKAKQSRGEKKARKIMLKLGLKQIQGVNRVTIRKSKNILFVINNPDVYKNPHSDTYIVFGEAKIEDLSQQAQVAAAEKFKAPEAAGAADSVGATTSVAPIAEEDEEDV-----DDTGVDEKDIELVITQANTTRAKAIKALKNNNNDIVNAIMELT 215

[Close]

BLAST of SMED30017018 vs. Ensembl Fly
Match: CG4415 (gene:FBgn0031296 transcript:FBtr0301087)

HSP 1 Score: 90.5077 bits (223), Expect = 3.647e-20
Identity = 68/163 (41.72%), Postives = 87/163 (53.37%), Query Frame = -3

Query:  353 SKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESD---------GEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 814
            SKQSR E+KAR+ + KL LK +  V RV ++K KNIL  + QP+VY+   S TY+ FGE  +ED S  +  + A   A  +                  +   EES          GED DA GL   DI+L+  QA  SR KAI+AL   DND+VNAIM LT
Sbjct:  186 SKQSRGERKARRILMKLDLKPMENVARVTMKKGKNILLYIDQPDVYRVAHSSTYIFFGEICVEDTSTTSVASQAAVKAAERFRGTSPAGRQGNEDG---KAPGEESPHDDDDDENDGEDADAMGLHAKDIELVQMQAACSRKKAIQALLKNDNDVVNAIMALT 345

[Close]

BLAST of SMED30017018 vs. Ensembl Zebrafish
Match: naca (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:195823])

HSP 1 Score: 163.696 bits (413), Expect = 1.009e-48
Identity = 101/160 (63.12%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ + GV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVAGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. Ensembl Zebrafish
Match: naca (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:195823])

HSP 1 Score: 163.696 bits (413), Expect = 1.127e-48
Identity = 101/160 (63.12%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ + GV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:   64 EEPVSKAKQSRSEKKARKAMSKLGLRQVAGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 217

[Close]

BLAST of SMED30017018 vs. Ensembl Zebrafish
Match: naca (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:195823])

HSP 1 Score: 169.859 bits (429), Expect = 1.524e-46
Identity = 101/160 (63.12%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ + GV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:  876 EEPVSKAKQSRSEKKARKAMSKLGLRQVAGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 1029

[Close]

BLAST of SMED30017018 vs. Ensembl Zebrafish
Match: nacad (NAC alpha domain containing [Source:ZFIN;Acc:ZDB-GENE-091020-7])

HSP 1 Score: 139.813 bits (351), Expect = 1.962e-39
Identity = 86/161 (53.42%), Postives = 112/161 (69.57%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQ-EESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            D+ +S++KQSRSEKKARKAMSKLGLK I GV R+ IRKSKNILFV+ +P+V+KSPASD Y+VFGEAKIED          +  A+     +D +   +     +  TI+ E  + E+ D  GL + DI+L+M QA VSR KA+RAL++  ND+VNAIMELT
Sbjct:   75 DESVSRAKQSRSEKKARKAMSKLGLKQIHGVTRITIRKSKNILFVITRPDVFKSPASDIYIVFGEAKIED----LSQQVHKAAAEKFKVPLDPSPLPSDITPSL--TIKEESEEEEELDEGGLEQRDIELVMAQANVSRAKAVRALRHNKNDIVNAIMELT 229

[Close]

BLAST of SMED30017018 vs. Ensembl Zebrafish
Match: nacad (NAC alpha domain containing [Source:ZFIN;Acc:ZDB-GENE-091020-7])

HSP 1 Score: 139.043 bits (349), Expect = 8.020e-36
Identity = 86/161 (53.42%), Postives = 112/161 (69.57%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQ-EESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            D+ +S++KQSRSEKKARKAMSKLGLK I GV R+ IRKSKNILFV+ +P+V+KSPASD Y+VFGEAKIED          +  A+     +D +   +     +  TI+ E  + E+ D  GL + DI+L+M QA VSR KA+RAL++  ND+VNAIMELT
Sbjct: 1715 DESVSRAKQSRSEKKARKAMSKLGLKQIHGVTRITIRKSKNILFVITRPDVFKSPASDIYIVFGEAKIED----LSQQVHKAAAEKFKVPLDPSPLPSDITPSL--TIKEESEEEEELDEGGLEQRDIELVMAQANVSRAKAVRALRHNKNDIVNAIMELT 1869

[Close]

BLAST of SMED30017018 vs. Ensembl Xenopus
Match: aldoa (aldolase, fructose-bisphosphate A [Source:Xenbase;Acc:XB-GENE-959791])

HSP 1 Score: 166.007 bits (419), Expect = 3.281e-49
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   83 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 236

[Close]

BLAST of SMED30017018 vs. Ensembl Xenopus
Match: aldoa (aldolase, fructose-bisphosphate A [Source:Xenbase;Acc:XB-GENE-959791])

HSP 1 Score: 166.777 bits (421), Expect = 3.773e-49
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:  112 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 265

[Close]

BLAST of SMED30017018 vs. Ensembl Xenopus
Match: aldoa (aldolase, fructose-bisphosphate A [Source:Xenbase;Acc:XB-GENE-959791])

HSP 1 Score: 167.548 bits (423), Expect = 4.068e-49
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:  135 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 288

[Close]

BLAST of SMED30017018 vs. Ensembl Xenopus
Match: aldoa (aldolase, fructose-bisphosphate A [Source:Xenbase;Acc:XB-GENE-959791])

HSP 1 Score: 172.94 bits (437), Expect = 1.121e-47
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:  682 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 835

[Close]

BLAST of SMED30017018 vs. Ensembl Xenopus
Match: aldoa (aldolase, fructose-bisphosphate A [Source:Xenbase;Acc:XB-GENE-959791])

HSP 1 Score: 173.326 bits (438), Expect = 1.418e-47
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:  795 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 948

[Close]

BLAST of SMED30017018 vs. Ensembl Mouse
Match: Naca (nascent polypeptide-associated complex alpha polypeptide [Source:MGI Symbol;Acc:MGI:106095])

HSP 1 Score: 165.622 bits (418), Expect = 1.816e-49
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. Ensembl Mouse
Match: Naca (nascent polypeptide-associated complex alpha polypeptide [Source:MGI Symbol;Acc:MGI:106095])

HSP 1 Score: 171.4 bits (433), Expect = 5.340e-47
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct: 2033 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 2186

[Close]

BLAST of SMED30017018 vs. Ensembl Mouse
Match: Nacad (NAC alpha domain containing [Source:MGI Symbol;Acc:MGI:3603030])

HSP 1 Score: 139.043 bits (349), Expect = 6.882e-36
Identity = 96/168 (57.14%), Postives = 118/168 (70.24%), Query Frame = -3

Query:  353 ADGATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 856
            A G  G +++ ++K+KQSRSEKKARKAMSKLGL+ I GV R+ I+KSKNILFV+ +P+V+KSPASDTYVVFGEAKIED+S Q   AAAEK              S  P        QEE D E E+A GL   DI+L+M QA V+R KA+RALK+  +D+VNAIMELT
Sbjct: 1337 AQGPAGSNEETIAKAKQSRSEKKARKAMSKLGLRQIQGVTRITIQKSKNILFVIAKPDVFKSPASDTYVVFGEAKIEDLSQQVHKAAAEKFKVPSEPSALVPELSPGPRVRPECEEQEEEDEEVEEA-GLEPRDIELVMAQANVTRAKAVRALKDNHSDIVNAIMELT 1503

[Close]

BLAST of SMED30017018 vs. UniProt/SwissProt
Match: sp|Q60817|NACA_MOUSE (Nascent polypeptide-associated complex subunit alpha OS=Mus musculus OX=10090 GN=Naca PE=1 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 1.270e-48
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. UniProt/SwissProt
Match: sp|Q13765|NACA_HUMAN (Nascent polypeptide-associated complex subunit alpha OS=Homo sapiens OX=9606 GN=NACA PE=1 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 1.270e-48
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. UniProt/SwissProt
Match: sp|Q8AWF2|NACA_ORENI (Nascent polypeptide-associated complex subunit alpha OS=Oreochromis niloticus OX=8128 GN=naca PE=2 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 1.312e-48
Identity = 103/160 (64.38%), Postives = 125/160 (78.12%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V   T S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVPGETVSNVQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. UniProt/SwissProt
Match: sp|Q5E9A1|NACA_BOVIN (Nascent polypeptide-associated complex subunit alpha OS=Bos taurus OX=9913 GN=NACA PE=1 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 1.771e-48
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. UniProt/SwissProt
Match: sp|Q68F90|NACA_XENTR (Nascent polypeptide-associated complex subunit alpha OS=Xenopus tropicalis OX=8364 GN=naca PE=2 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 2.020e-48
Identity = 102/160 (63.75%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN  ND+VNAIMELT
Sbjct:   60 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELT 213

[Close]

BLAST of SMED30017018 vs. TrEMBL
Match: A0A161N1A8 (Nascent polypeptide-associated complex subunit alpha (Fragment) OS=Triatoma infestans OX=30076 PE=4 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 8.378e-50
Identity = 103/166 (62.05%), Postives = 126/166 (75.90%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            G+   H D +SK+KQSR EKKARK MSKLGLK I GV+RV IRKSKNILFV+ +P+V+K+PASDTY++FGE KIED+S QAQ+AAAEK         D + ++   A  VV  IQEES+ ED DATG+ E DI+L+M QA VSRNKA++AL N  ND+VNAIMELT
Sbjct:   33 GSVSLHPD-ISKAKQSRGEKKARKLMSKLGLKPIQGVSRVTIRKSKNILFVINKPDVFKNPASDTYIIFGETKIEDLSQQAQVAAAEKFKAP-----DISQSTETGATTVVAPIQEESEEEDVDATGIEEKDIELVMSQANVSRNKAVKALTNNQNDIVNAIMELT 192

[Close]

BLAST of SMED30017018 vs. TrEMBL
Match: A0A069DWV5 (Putative transcription factor OS=Panstrongylus megistus OX=65343 PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 8.572e-50
Identity = 103/166 (62.05%), Postives = 126/166 (75.90%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            G+   H D +SK+KQSR EKKARK MSKLGLK I GV+RV IRKSKNILFV+ +P+V+K+PASDTY++FGE KIED+S QAQ+AAAEK         D + ++   A  VV  IQEES+ ED DATG+ E DI+L+M QA VSRNKA++AL N  ND+VNAIMELT
Sbjct:   32 GSVSLHPD-ISKAKQSRGEKKARKLMSKLGLKPIQGVSRVTIRKSKNILFVINKPDVFKNPASDTYIIFGETKIEDLSQQAQVAAAEKFKAP-----DISQSTETGATTVVAPIQEESEEEDVDATGIEEKDIELVMSQANVSRNKAVKALTNNQNDIVNAIMELT 191

[Close]

BLAST of SMED30017018 vs. TrEMBL
Match: A0A224XL93 (Putative transcription factor OS=Panstrongylus lignarius OX=156445 PE=4 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 8.572e-50
Identity = 103/166 (62.05%), Postives = 126/166 (75.90%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            G+   H D +SK+KQSR EKKARK MSKLGLK I GV+RV IRKSKNILFV+ +P+V+K+PASDTY++FGE KIED+S QAQ+AAAEK         D + ++   A  VV  IQEES+ ED DATG+ E DI+L+M QA VSRNKA++AL N  ND+VNAIMELT
Sbjct:   32 GSVSLHPD-ISKAKQSRGEKKARKLMSKLGLKPIQGVSRVTIRKSKNILFVINKPDVFKNPASDTYIIFGETKIEDLSQQAQVAAAEKFKAP-----DISQSTETGATTVVAPIQEESEEEDVDATGIEEKDIELVMSQANVSRNKAVKALTNNQNDIVNAIMELT 191

[Close]

BLAST of SMED30017018 vs. TrEMBL
Match: T1IDE0 (NAC-A/B domain-containing protein OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 8.572e-50
Identity = 103/166 (62.05%), Postives = 126/166 (75.90%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            G+   H D +SK+KQSR EKKARK MSKLGLK I GV+RV IRKSKNILFV+ +P+V+K+PASDTY++FGE KIED+S QAQ+AAAEK         D + ++   A  VV  IQEES+ ED DATG+ E DI+L+M QA VSRNKA++AL N  ND+VNAIMELT
Sbjct:   32 GSVSLHPD-ISKAKQSRGEKKARKLMSKLGLKPIQGVSRVTIRKSKNILFVINKPDVFKNPASDTYIIFGETKIEDLSQQAQVAAAEKFKAP-----DISQSTETGATTVVAPIQEESEEEDVDATGIEEKDIELVMSQANVSRNKAVKALTNNQNDIVNAIMELT 191

[Close]

BLAST of SMED30017018 vs. TrEMBL
Match: A0A023FAS8 (Putative transcription factor OS=Triatoma infestans OX=30076 PE=2 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 7.387e-49
Identity = 103/166 (62.05%), Postives = 125/166 (75.30%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
            G+   H D +SK+KQSR EKKARK MSKLGLK I GV+RV IRKSKNILFV+ +P+V K+PASDTY++FGE KIED+S QAQ+AAAEK         D + ++   A  VV  IQEES+ ED DATG+ E DI+L+M QA VSRNKA++AL N  ND+VNAIMELT
Sbjct:   32 GSVSLHPD-ISKAKQSRGEKKARKLMSKLGLKPIQGVSRVTIRKSKNILFVINKPDVXKNPASDTYIIFGETKIEDLSQQAQVAAAEKFKAP-----DISQSTETGATTVVAPIQEESEEEDVDATGIEEKDIELVMSQANVSRNKAVKALTNNQNDIVNAIMELT 191

[Close]

BLAST of SMED30017018 vs. Ensembl Cavefish
Match: ENSAMXT00000047756.1 (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:103024960])

HSP 1 Score: 166.007 bits (419), Expect = 1.114e-49
Identity = 103/160 (64.38%), Postives = 125/160 (78.12%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V    AS         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAASNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. Ensembl Cavefish
Match: ENSAMXT00000032505.1 (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:103024960])

HSP 1 Score: 166.777 bits (421), Expect = 2.147e-47
Identity = 103/160 (64.38%), Postives = 125/160 (78.12%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V    AS         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:  299 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAASNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 452

[Close]

BLAST of SMED30017018 vs. Ensembl Cavefish
Match: ENSAMXT00000055179.1 (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:103024960])

HSP 1 Score: 169.474 bits (428), Expect = 1.007e-46
Identity = 103/160 (64.38%), Postives = 125/160 (78.12%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V    AS         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:  634 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAASNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 787

[Close]

BLAST of SMED30017018 vs. Ensembl Cavefish
Match: ENSAMXT00000052916.1 (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:103024960])

HSP 1 Score: 168.703 bits (426), Expect = 2.908e-46
Identity = 103/160 (64.38%), Postives = 125/160 (78.12%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V    AS         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:  872 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAASNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 1025

[Close]

BLAST of SMED30017018 vs. Ensembl Cavefish
Match: ENSAMXT00000036282.1 (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:103024960])

HSP 1 Score: 168.703 bits (426), Expect = 2.908e-46
Identity = 103/160 (64.38%), Postives = 125/160 (78.12%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V    AS         T+QEES+ E+ D TG+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:  872 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAASNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 1025

[Close]

BLAST of SMED30017018 vs. Ensembl Sea Lamprey
Match: naca (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:195823])

HSP 1 Score: 147.517 bits (371), Expect = 3.037e-43
Identity = 91/167 (54.49%), Postives = 114/167 (68.26%), Query Frame = -3

Query:  353 HDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCE------NDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 835
            +++ +SK+KQSRSEKKARKAMSKLGL+ + GV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AA            +        A  V E +Q     E+ +     +       DI+L+M QA VSR+KA+RALKN  ND+VNAIMELT
Sbjct:   62 NEELVSKAKQSRSEKKARKAMSKLGLRQVGGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQMAA-----------AEKFKVPGEAAPGVQERVQPPPVPEESEEEEEVDETGVEAKDIELVMSQANVSRSKAVRALKNNSNDIVNAIMELT 217

[Close]

BLAST of SMED30017018 vs. Ensembl Yeast
Match: EGD2 (Alpha subunit of the nascent polypeptide-associated complex (NAC); involved in protein sorting and translocation; associated with cytoplasmic ribosomes [Source:SGD;Acc:S000001236])

HSP 1 Score: 79.7221 bits (195), Expect = 2.265e-18
Identity = 56/167 (33.53%), Postives = 89/167 (53.29%), Query Frame = -3

Query:  353 SRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATG----------------LCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 805
            +++EKKAR+ + KLGLK I G+ RV  RK  N ++ + +PEV++S A   YVVFGEAK+++ + +   A  +  A       +     +       E IQ +     E +                  L ++DI+L++ Q  VS+N+AI+ALK  + D+VNAIM L+
Sbjct:   14 NKNEKKARELIGKLGLKQIPGIIRVTFRKKDNQIYAIEKPEVFRS-AGGNYVVFGEAKVDNFTQKLAAAQQQAQASGIMPSNEDVATKSP------EDIQADMQAAAEGSVNAAAEEDDEEGEVDAGDLNKDDIELVVQQTNVSKNQAIKALKAHNGDLVNAIMSLS 173

[Close]

BLAST of SMED30017018 vs. Ensembl Nematostella
Match: EDO45869 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRQ8])

HSP 1 Score: 156.762 bits (395), Expect = 9.142e-47
Identity = 98/166 (59.04%), Postives = 122/166 (73.49%), Query Frame = -3

Query:  353 GATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 850
             ATG +++P+SK+KQSR EKKARKAM KL LK +TGVNRV IRKSKNILF++ +P+VYK+PASD Y++FGEAKIED+S QAQ+ AAEK       +      +   A P VE  +EE +  DE  TG+   DI+L+M QA VSRNKAI ALK  +ND+VNAIMELT
Sbjct:   45 AATGLNEEPVSKAKQSRGEKKARKAMQKLSLKSVTGVNRVTIRKSKNILFIINKPDVYKNPASDMYIIFGEAKIEDLSQQAQVQAAEKF------KAPNPAPTIPEAKPAVEQEEEEEEAIDE--TGVESKDIELVMQQANVSRNKAIMALKKNENDIVNAIMELT 202

[Close]

BLAST of SMED30017018 vs. Ensembl Medaka
Match: NACA (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:101162050])

HSP 1 Score: 163.696 bits (413), Expect = 8.321e-49
Identity = 101/160 (63.12%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D  G+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:   61 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDEAGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 214

[Close]

BLAST of SMED30017018 vs. Ensembl Medaka
Match: NACA (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:101162050])

HSP 1 Score: 168.703 bits (426), Expect = 3.046e-46
Identity = 101/160 (63.12%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D  G+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:  853 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDEAGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 1006

[Close]

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D  G+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:  853 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDEAGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 1006

[Close]

BLAST of SMED30017018 vs. Ensembl Medaka
Match: NACA (nascent polypeptide associated complex subunit alpha [Source:NCBI gene;Acc:101162050])

HSP 1 Score: 168.318 bits (425), Expect = 3.714e-46
Identity = 101/160 (63.12%), Postives = 123/160 (76.88%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            ++P+SK+KQSRSEKKARKAMSKLGL+ +TGV RV IRKSKNILFV+ +P+VYKSPASDTY+VFGEAKIED+S QAQ+AAAEK       +V     S         T+QEES+ E+ D  G+   DI+L+M QA VSR KA+RALKN +ND+VNAIMELT
Sbjct:  744 EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKF------KVQGEAVSNIQENTQTPTVQEESEEEEVDEAGVEVKDIELVMSQANVSRAKAVRALKNNNNDIVNAIMELT 897

[Close]

BLAST of SMED30017018 vs. Ensembl Medaka
Match: ENSORLT00000034684.1 (uncharacterized LOC101166002 [Source:NCBI gene;Acc:101166002])

HSP 1 Score: 137.887 bits (346), Expect = 1.468e-35
Identity = 94/162 (58.02%), Postives = 118/162 (72.84%), Query Frame = -3

Query:  353 DDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQ--EESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELT 832
            DD L++ KQSRSEKKARKAMSKLGLK + GV R+ IRKSK+ILFV+ +P+V+KSP SD Y+VFGEAKIED+S QA  AAAEK       +V   ++  A   P   TI+   E + E+ D  GL + DI+L+M QA VSR KA+RALK+ +ND+VNAIMELT
Sbjct: 1943 DDTLNRPKQSRSEKKARKAMSKLGLKPVHGVTRITIRKSKSILFVISRPDVFKSPVSDIYIVFGEAKIEDLSQQAHKAAAEKF------KVPMMSSPLATPVPPSLTIKEESEEEEEEVDEGGLEQRDIELVMAQANVSRVKAVRALKHNNNDIVNAIMELT 2098

[Close]

BLAST of SMED30017018 vs. Planmine SMEST
Match: SMESG000007294.1 (SMESG000007294.1)

HSP 1 Score: 280.026 bits (715), Expect = 1.269e-94
Identity = 198/200 (99.00%), Postives = 200/200 (100.00%), Query Frame = -3

Query:  350 MGLETDQKXXXXXXXXXXXXXXXXXXXXXVKADGATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDXXXXXXXXXXXXXXXXXATEVDXXXXXXXXXXPVVETIQEESDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELTN 949
            MGLETDQKVHEVVSESESEESVPELESGEVKADGATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDISAQAQIAAAEKIAKSKATEVDTTTASAAPAAPVV+TIQEESDGE+EDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELTN
Sbjct:    1 MGLETDQKVHEVVSESESEESVPELESGEVKADGATGDHDDPLSKSKQSRSEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYVVFGEAKIEDISAQAQIAAAEKIAKSKATEVDTTTASAAPAAPVVDTIQEESDGEEEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELTN 200

[Close]

BLAST of SMED30017018 vs. Planmine SMEST
Match: SMESG000016070.1 (SMESG000016070.1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.603e-16
Identity = 43/71 (60.56%), Postives = 56/71 (78.87%), Query Frame = 3

Query:   21 KSICPNXXXXXXXXXXXXXXXX-XSFIVAATEVLLVPFCACSENAKRICERLDRLYKILAFQPIDNMMKMR 230
            KS CPN+++S LWF L++     LSFIVA  EVL VPFCAC E AK+ICE LDR++KI+A+QPI+NM+ M+
Sbjct:    3 KSFCPNILYSILWFTLWIFFAFPLSFIVAFIEVLFVPFCACFEKAKQICEVLDRMFKIMAYQPIENMLNMK 73

[Close]

BLAST of SMED30017018 vs. Planmine SMEST
Match: SMESG000065952.1 (SMESG000065952.1)

HSP 1 Score: 62.003 bits (149), Expect = 3.840e-12
Identity = 26/47 (55.32%), Postives = 38/47 (80.85%), Query Frame = 3

Query:   90 SFIVAATEVLLVPFCACSENAKRICERLDRLYKILAFQPIDNMMKMR 230
            +F +A  EV ++PFCAC E AK+ICE LDR++K+LA+QP++NM+  R
Sbjct:   25 AFFLALVEVAVIPFCACLEPAKQICEVLDRIFKVLAYQPMENMIYGR 71

[Close]

BLAST of SMED30017018 vs. Planmine SMEST
Match: SMESG000065952.1 (SMESG000065952.1)

HSP 1 Score: 62.003 bits (149), Expect = 3.900e-12
Identity = 26/47 (55.32%), Postives = 38/47 (80.85%), Query Frame = 3

Query:   90 SFIVAATEVLLVPFCACSENAKRICERLDRLYKILAFQPIDNMMKMR 230
            +F +A  EV ++PFCAC E AK+ICE LDR++K+LA+QP++NM+  R
Sbjct:   26 AFFLALVEVAVIPFCACLEPAKQICEVLDRIFKVLAYQPMENMIYGR 72

The following BLAST results are available for this feature:

BLAST of SMED30017018 vs. Ensembl Human
BLAST of SMED30017018 vs. Ensembl Celegans
BLAST of SMED30017018 vs. Ensembl Fly
BLAST of SMED30017018 vs. Ensembl Zebrafish
BLAST of SMED30017018 vs. Ensembl Xenopus
BLAST of SMED30017018 vs. Ensembl Mouse
BLAST of SMED30017018 vs. UniProt/SwissProt
BLAST of SMED30017018 vs. TrEMBL
BLAST of SMED30017018 vs. Ensembl Cavefish
BLAST of SMED30017018 vs. Ensembl Sea Lamprey
BLAST of SMED30017018 vs. Ensembl Yeast
BLAST of SMED30017018 vs. Ensembl Nematostella
BLAST of SMED30017018 vs. Ensembl Medaka
BLAST of SMED30017018 vs. Planmine SMEST

BLAST of SMED30017018 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
NACA	2.645e-49	63.75	nascent polypeptide associated complex subunit alp...	[more]
NACA	2.645e-49	63.75	nascent polypeptide associated complex subunit alp...	[more]
NACA	2.645e-49	63.75	nascent polypeptide associated complex subunit alp...	[more]
NACA	2.645e-49	63.75	nascent polypeptide associated complex subunit alp...	[more]
NACA	4.661e-49	63.75	nascent polypeptide associated complex subunit alp...	[more]

BLAST of SMED30017018 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
icd-2	7.309e-45	56.44	Nascent polypeptide-associated complex subunit alp...	[more]
icd-2	3.432e-43	53.99	Nascent polypeptide-associated complex subunit alp...	[more]
icd-2	4.272e-43	53.99	Nascent polypeptide-associated complex subunit alp...	[more]

BLAST of SMED30017018 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Nacalpha	6.196e-43	59.04	gene:FBgn0086904 transcript:FBtr0087858	[more]
Nacalpha	6.196e-43	59.04	gene:FBgn0086904 transcript:FBtr0087859	[more]
Nacalpha	6.196e-43	59.04	gene:FBgn0086904 transcript:FBtr0345195	[more]
Nacalpha	6.196e-43	59.04	gene:FBgn0086904 transcript:FBtr0087860	[more]
CG4415	3.647e-20	41.72	gene:FBgn0031296 transcript:FBtr0301087	[more]

BLAST of SMED30017018 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
naca	1.009e-48	63.13	nascent polypeptide associated complex subunit alp...	[more]
naca	1.127e-48	63.13	nascent polypeptide associated complex subunit alp...	[more]
naca	1.524e-46	63.13	nascent polypeptide associated complex subunit alp...	[more]
nacad	1.962e-39	53.42	NAC alpha domain containing [Source:ZFIN;Acc:ZDB-G...	[more]
nacad	8.020e-36	53.42	NAC alpha domain containing [Source:ZFIN;Acc:ZDB-G...	[more]

BLAST of SMED30017018 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
aldoa	3.281e-49	63.75	aldolase, fructose-bisphosphate A [Source:Xenbase;...	[more]
aldoa	3.773e-49	63.75	aldolase, fructose-bisphosphate A [Source:Xenbase;...	[more]
aldoa	4.068e-49	63.75	aldolase, fructose-bisphosphate A [Source:Xenbase;...	[more]
aldoa	1.121e-47	63.75	aldolase, fructose-bisphosphate A [Source:Xenbase;...	[more]
aldoa	1.418e-47	63.75	aldolase, fructose-bisphosphate A [Source:Xenbase;...	[more]

BLAST of SMED30017018 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Naca	1.816e-49	63.75	nascent polypeptide-associated complex alpha polyp...	[more]
Naca	5.340e-47	63.75	nascent polypeptide-associated complex alpha polyp...	[more]
Nacad	6.882e-36	57.14	NAC alpha domain containing [Source:MGI Symbol;Acc...	[more]

BLAST of SMED30017018 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q60817\|NACA_MOUSE	1.270e-48	63.75	Nascent polypeptide-associated complex subunit alp...	[more]
sp\|Q13765\|NACA_HUMAN	1.270e-48	63.75	Nascent polypeptide-associated complex subunit alp...	[more]
sp\|Q8AWF2\|NACA_ORENI	1.312e-48	64.38	Nascent polypeptide-associated complex subunit alp...	[more]
sp\|Q5E9A1\|NACA_BOVIN	1.771e-48	63.75	Nascent polypeptide-associated complex subunit alp...	[more]
sp\|Q68F90\|NACA_XENTR	2.020e-48	63.75	Nascent polypeptide-associated complex subunit alp...	[more]

BLAST of SMED30017018 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A161N1A8	8.378e-50	62.05	Nascent polypeptide-associated complex subunit alp...	[more]
A0A069DWV5	8.572e-50	62.05	Putative transcription factor OS=Panstrongylus meg...	[more]
A0A224XL93	8.572e-50	62.05	Putative transcription factor OS=Panstrongylus lig...	[more]
T1IDE0	8.572e-50	62.05	NAC-A/B domain-containing protein OS=Rhodnius prol...	[more]
A0A023FAS8	7.387e-49	62.05	Putative transcription factor OS=Triatoma infestan...	[more]

BLAST of SMED30017018 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000047756.1	1.114e-49	64.38	nascent polypeptide associated complex subunit alp...	[more]
ENSAMXT00000032505.1	2.147e-47	64.38	nascent polypeptide associated complex subunit alp...	[more]
ENSAMXT00000055179.1	1.007e-46	64.38	nascent polypeptide associated complex subunit alp...	[more]
ENSAMXT00000052916.1	2.908e-46	64.38	nascent polypeptide associated complex subunit alp...	[more]
ENSAMXT00000036282.1	2.908e-46	64.38	nascent polypeptide associated complex subunit alp...	[more]

BLAST of SMED30017018 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
naca	3.037e-43	54.49	nascent polypeptide associated complex subunit alp...	[more]

BLAST of SMED30017018 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
EGD2	2.265e-18	33.53	Alpha subunit of the nascent polypeptide-associate...	[more]

BLAST of SMED30017018 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO45869	9.142e-47	59.04	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30017018 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
NACA	8.321e-49	63.13	nascent polypeptide associated complex subunit alp...	[more]
NACA	3.046e-46	63.13	nascent polypeptide associated complex subunit alp...	[more]
NACA	3.046e-46	63.13	nascent polypeptide associated complex subunit alp...	[more]
NACA	3.714e-46	63.13	nascent polypeptide associated complex subunit alp...	[more]
ENSORLT00000034684.1	1.468e-35	58.02	uncharacterized LOC101166002 [Source:NCBI gene;Acc...	[more]

BLAST of SMED30017018 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000007294.1	1.269e-94	99.00	SMESG000007294.1	[more]
SMESG000016070.1	1.603e-16	60.56	SMESG000016070.1	[more]
SMESG000065952.1	3.840e-12	55.32	SMESG000065952.1	[more]
SMESG000065952.1	3.900e-12	55.32	SMESG000065952.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence
protein sequence

transcript sequence

>SMED30017018 ID=SMED30017018|Name=SMED30017018|organism=Schmidtea mediterranea sexual|type=transcript|length=1008bp
ATAAATCATCTATGGTACCAAAATCAATATGTCCAAATTTGATATGGTCA
TTTTTATGGTTCATTTTATTTTTGATAACATGGCCACTATCCTTCATTGT
AGCTGCTACCGAAGTCCTGTTAGTACCATTTTGTGCATGTTCTGAAAATG
CCAAAAGAATATGTGAAAGGCTTGACAGATTATACAAAATTTTAGCATTT
CAGCCCATCGATAACATGATGAAAATGAGAAACTGTATAATAAATGACAC
TAACAATGAAATAACTGAAGATAATGAACAAATACCACCAGAAAATGATA
ACTAACACTGAAATTTCCAAAATTTTATTAAATACAACATTTGTAATTAG
TTGGTTAATTCCATAATAGCATTGACCATATCGTTATCTGCATTTTTCAA
AGCTCGAATAGCTTTATTTCTACTAACTTGAGCTTGATCCATAATCAATT
TAATGTCATTTTCACAAAGCCCTGTGGCATCTTCGTCTTCTCCATCTGAT
TCTTCTTGAATAGTTTCAACGACGGGTGCAGCTGGAGCGGCTGATGCAGT
GGTAGTGTCAACTTCAGTTGCTTTGGATTTAGCAATTTTTTCAGCTGCTG
CAATTTGAGCTTGCGCTGAAATATCTTCTATTTTAGCTTCTCCAAAAACT
ACGTATGTATCGGATGCAGGTGATTTATAAACTTCTGGTTGACCAACAAC
AAATAAAATATTTTTAGATTTTCGAATGGCGACTCGATTAACTCCTGTAA
TATGTTTCAAACCCAATTTGCTCATAGCTTTTCTAGCTTTTTTTTCGCTT
CTAGATTGTTTACTTTTACTTAAAGGATCGTCATGGTCACCAGTTGCACC
ATCGGCTTTAACTTCCCCAGATTCTAATTCTGGTACAGATTCCTCAGATT
CAGATTCTGAGACCACTTCGTGAACCTTTTGATCAGTTTCCAATCCCATT
AGTTAAAAATTCAATTTCAATGTTTATTAAAAAATTAAAACAAAATTTAA
ATTATGAG

protein sequence of SMED30017018-orf-1

>SMED30017018-orf-1 ID=SMED30017018-orf-1|Name=SMED30017018-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=101bp
KSSMVPKSICPNLIWSFLWFILFLITWPLSFIVAATEVLLVPFCACSENA
KRICERLDRLYKILAFQPIDNMMKMRNCIINDTNNEITEDNEQIPPENDN
*

protein sequence of SMED30017018-orf-2

>SMED30017018-orf-2 ID=SMED30017018-orf-2|Name=SMED30017018-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=201bp
MGLETDQKVHEVVSESESEESVPELESGEVKADGATGDHDDPLSKSKQSR
SEKKARKAMSKLGLKHITGVNRVAIRKSKNILFVVGQPEVYKSPASDTYV
VFGEAKIEDISAQAQIAAAEKIAKSKATEVDTTTASAAPAAPVVETIQEE
SDGEDEDATGLCENDIKLIMDQAQVSRNKAIRALKNADNDMVNAIMELTN
*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000429	neoblast

Vocabulary: **INTERPRO**
Term	Definition
IPR038187	NAC_A/B_dom_sf
IPR002715	Nas_poly-pep-assoc_cplx_dom
IPR016641	EGD2/NACA
IPR009060	UBA-like_sf

Vocabulary: **cellular component**
Term	Definition
GO:0005854	nascent polypeptide-associated complex

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 170..190
None	No IPR available	GENE3D	G3DSA:1.10.8.10		coord: 161..199 e-value: 2.7E-13 score: 51.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..59
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..58
None	No IPR available	CDD	cd14358	UBA_NAC_euk	coord: 163..199 e-value: 9.11434E-16 score: 66.1075
IPR002715	Nascent polypeptide-associated complex NAC domain	SMART	SM01407	NAC_2	coord: 52..108 e-value: 1.6E-25 score: 100.8
IPR002715	Nascent polypeptide-associated complex NAC domain	PFAM	PF01849	NAC	coord: 52..107 e-value: 7.4E-23 score: 80.3
IPR002715	Nascent polypeptide-associated complex NAC domain	PROSITE	PS51151	NAC_AB	coord: 49..114 score: 18.545
IPR038187	NAC A/B domain superfamily	GENE3D	G3DSA:2.20.70.30		coord: 58..111 e-value: 6.6E-30 score: 104.4
IPR016641	Nascent polypeptide-associated complex subunit alpha	PIRSF	PIRSF015901	NAC_alpha	coord: 8..200 e-value: 2.5E-65 score: 217.3
IPR016641	Nascent polypeptide-associated complex subunit alpha	PANTHER	PTHR21713	NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED	coord: 13..199
IPR009060	UBA-like superfamily	SUPERFAMILY	SSF46934	UBA-like	coord: 161..199