Deferrochelatase/peroxidase YfeX

Overview
NameDeferrochelatase/peroxidase YfeX
Smed IDSMED30017015
Length (bp)1344
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Deferrochelatase/peroxidase YfeX (SMED30017015) t-SNE clustered cells

Violin plots show distribution of expression levels for Deferrochelatase/peroxidase YfeX (SMED30017015) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Deferrochelatase/peroxidase YfeX (SMED30017015) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Deferrochelatase/peroxidase YfeX (SMED30017015) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30017015

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30017015SMESG000077723.1 dd_Smed_v4_2999_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30017015SMESG000077723.1 dd_Smed_v4_2999_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30017015SMESG000077723.1 dd_Smed_v4_2999_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017015SMESG000077723.1 Contig4686newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017015SMESG000077723.1 Contig4686uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017015SMESG000077723.1 Contig30864newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017015SMESG000077723.1 Contig30864uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017015SMESG000077723.1 Contig34449newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017015SMESG000077723.1 Contig34449uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017015SMESG000077723.1 Contig52155newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30017015SMESG000077723.1 Contig52155uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Deferrochelatase/peroxidase YfeX vs. UniProt/SwissProt
Match: sp|Q8XBI9|YFEX_ECO57 (Dye-decolorizing peroxidase YfeX OS=Escherichia coli O157:H7 OX=83334 GN=yfeX PE=1 SV=2)

HSP 1 Score: 124.405 bits (311), Expect = 4.349e-31
Identity = 94/310 (30.32%), Postives = 148/310 (47.74%), Query Frame = 3 
Query:  303 QESVFSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRXXXXXXXXXXY--YTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAV---NTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTEL--KRKSTTSHVARMTGGPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDLL 1211
            Q  +  +    A+++  +++ EV         A  K + DK++    +  +  + A V FG N    +           +  Y +    G  P+T  D+ IH         F +AQ  ++    + IE  E+I+G+ +   RDLSGF+DGTENPA  + R+EVAV       GGSYV  Q+W H    +N+      E  +GR +  + E+    +  TSH+ R+      E  K  +IVRQS P+GT SG +GL+F  Y A +  ++  L  M G T DG  D +++ +K V   Y++ P    LD L+
Sbjct:    5 QSGILPEHCRAAIWIEANVKGEVDALR-----AASKTFADKLATFEAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGK----GLAPTTQFDVLIHILSLRHDVNFSVAQAAMEAFG-DCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVIKDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTRVD---LKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDT-DGKRDAMLRFTKPVTGGYYFAP---SLDKLM 297
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BLAST of Deferrochelatase/peroxidase YfeX vs. UniProt/SwissProt
Match: sp|P76536|YFEX_ECOLI (Dye-decolorizing peroxidase YfeX OS=Escherichia coli (strain K12) OX=83333 GN=yfeX PE=1 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 9.109e-31
Identity = 94/310 (30.32%), Postives = 148/310 (47.74%), Query Frame = 3 
Query:  303 QESVFSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRXXXXXXXXXXY--YTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAV---NTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTEL--KRKSTTSHVARMTGGPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDLL 1211
            Q  +  +    A+++  +++ EV         A  K + DK++    +  +  + A V FG N    +           +  Y +    G  P+T  D+ IH         F +AQ  ++    + IE  E+I+G+ +   RDLSGF+DGTENPA  + R+EVAV       GGSYV  Q+W H    +N+      E  +GR +  + E+    +  TSH+ R+      E  K  +IVRQS P+GT SG +GL+F  Y A +  ++  L  M G T DG  D +++ +K V   Y++ P    LD L+
Sbjct:    5 QSGILPEHCRAAIWIEANVKGEVDALR-----AASKTFADKLATFEAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGK----GLAPTTQFDVLIHILSLRHDVNFSVAQAAMEAFG-DCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVIKDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMVIGRTKEANEEIDGDERPETSHLTRVD---LKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDT-DGKRDAMLRFTKPVTGGYYFAP---SLDKLM 297
[Close]
BLAST of Deferrochelatase/peroxidase YfeX vs. TrEMBL
Match: A0A1S3JN88 (uncharacterized protein LOC106174718 OS=Lingula unguis OX=7574 GN=LOC106174718 PE=4 SV=1)

HSP 1 Score: 400.979 bits (1029), Expect = 2.991e-134
Identity = 193/328 (58.84%), Postives = 243/328 (74.09%), Query Frame = 3 
Query:  225 YTLFDRNKLKLTLNINPAFCASLLKSQESVFSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRXXXXXXXXXXYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTGGPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDL 1208
            Y L+  +  K  L + P   A+L +SQ SVFSQTK HALYL IHL+PE   K   K VANL+K+VD + P   R +++E+ AGVGFGPN  +++   +     ++Y  R+G  GEMPSTGGDIF+HAKCDE S+LFEL Q ILK LP++S++ +ED+Y +V+RYGRDLSGFIDGTENPA  + RQEVAV   TGGSYVITQKWIH+  VIN  K+ V+EG+VGR R DSTELK+K  +SHVARMTGG  F Q KKFEIVRQS P+G+LS + GLFFIGY+A+ +  ++ML+RMVGA  D   DD+M++S CVK TYWYFPG +EL  L
Sbjct:   56 YKLYGGDVPKHAL-LQPVHAATLPESQPSVFSQTKQHALYLWIHLKPEADSKACAKVVANLQKHVDAVDPPADRTEDSEIWAGVGFGPNCYSQL--GRKTKQDYAYRHRQGPLGEMPSTGGDIFVHAKCDEQSKLFELCQQILKSLPKDSVKEYEDVYSFVFRYGRDLSGFIDGTENPADDESRQEVAVEKETGGSYVITQKWIHRMNVINSTKDSVMEGWVGRSREDSTELKKKPISSHVARMTGGNAFAQPKKFEIVRQSMPYGSLSDKCGLFFIGYAAHPQNFEFMLDRMVGAGGDPHSDDVMRLSDCVKGTYWYFPGAEELKKL 380
[Close]
BLAST of Deferrochelatase/peroxidase YfeX vs. TrEMBL
Match: A0A1S3J2Z2 (uncharacterized protein LOC106169733 OS=Lingula unguis OX=7574 GN=LOC106169733 PE=4 SV=1)

HSP 1 Score: 399.823 bits (1026), Expect = 7.305e-134
Identity = 193/328 (58.84%), Postives = 242/328 (73.78%), Query Frame = 3 
Query:  225 YTLFDRNKLKLTLNINPAFCASLLKSQESVFSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRXXXXXXXXXXYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTGGPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDL 1208
            Y L+  +  K  L + P   A+L +SQ SVFSQTK HALYL IHL+PE   K   K VANL+K+VD + P   R +++E+ AGVGFGPN  +++   +     ++Y  R+G  GEMPSTGGDIF+HAKCDE S+LFEL Q ILK LP++S++ +ED+Y +V+RYGRDLSGFIDGTENPA  + RQEVAV   TGGSYVITQKWIH+  VIN  K+ V+EG+VGR R DSTELK+K  +SHVARMTGG  F Q KKFEIVRQS P+G LS + GLFFIGY+A+ +  ++ML+RMVGA  D   DD+M++S CVK TYWYFPG +EL  L
Sbjct:   56 YKLYGGDVPKHAL-LQPVHAATLPESQPSVFSQTKQHALYLWIHLKPEADSKACAKVVANLQKHVDAVDPPADRTEDSEIWAGVGFGPNCYSQL--GRKTKQDYAYRHRQGPLGEMPSTGGDIFVHAKCDEQSKLFELCQQILKSLPKDSVKEYEDVYSFVFRYGRDLSGFIDGTENPADDESRQEVAVEKETGGSYVITQKWIHRMNVINSTKDSVMEGWVGRSREDSTELKKKPISSHVARMTGGNAFAQPKKFEIVRQSMPYGNLSDKCGLFFIGYAAHPQNFEFMLDRMVGAGGDPHSDDVMRLSDCVKGTYWYFPGAEELKKL 380
[Close]
BLAST of Deferrochelatase/peroxidase YfeX vs. TrEMBL
Match: A0A194ALU0 (Uncharacterized protein OS=Pinctada fucata OX=50426 PE=4 SV=1)

HSP 1 Score: 389.423 bits (999), Expect = 9.186e-130
Identity = 197/359 (54.87%), Postives = 253/359 (70.47%), Query Frame = 3 
Query:  150 ARNKFTNSNKIILCGGAVTVFGGIFYTLFDRNKLKLTLN------INPAFCASLLKSQESVFSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRXXXXXXXXXXYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTGGPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDL 1208
            +R K   S+ ++L  G V V  G  Y ++  N+    L       +   F A + KSQ SV+S  K+HALYL IHL+PE + KEV K V NL+KYVD++    +RD+E+E+LAGVGFGPNF  +I        +++Y  RKG  G+MPS+GGD+FIHAKC+  S+LFELAQ+I+K +P  S+E +EDI  +VYR GRDLSGFIDGTEN A  ++RQ +AV    GGSYVITQKW+H   VI KEK++ LEG+VGR  SDSTEL RKS +SHVARMTGG  +EQKK+ EIVRQS PFG L+GE+GLFFIGY+ +    ++ML+RMVGA  D   DDIM++SK  K TYWYFPG++EL  L
Sbjct:   27 SRTKVRTSSALLL--GTVAVGAGS-YGIYKYNQRSNVLENLQDMLVPNVFAAGVPKSQPSVWSPGKNHALYLWIHLKPEANTKEVAKAVKNLQKYVDQVVDPAMRDEEDEILAGVGFGPNFFAQI--GGKALKNYNYPHRKGALGDMPSSGGDVFIHAKCNTTSKLFELAQVIMKNMPAGSVEKYEDIISFVYRNGRDLSGFIDGTENAADDENRQRIAVQEKNGGSYVITQKWLHDLNVIQKEKDKTLEGWVGRGISDSTELSRKSISSHVARMTGGNGWEQKKEAEIVRQSMPFGNLAGEHGLFFIGYAKDPYHFEFMLDRMVGAGSDNHCDDIMRLSKNTKGTYWYFPGEEELTKL 380
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BLAST of Deferrochelatase/peroxidase YfeX vs. TrEMBL
Match: V4C950 (Uncharacterized protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_114599 PE=4 SV=1)

HSP 1 Score: 379.022 bits (972), Expect = 6.990e-127
Identity = 181/299 (60.54%), Postives = 232/299 (77.59%), Query Frame = 3 
Query:  312 VFSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRXXXXXXXXXXYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTGGPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDL 1208
            V+S+ K+HALYL I+L+P  + +EV K VA ++KYVD+++   ++DD++E+LAGVGFGPNF +++  +     SF Y  RKG  G+MPS+GGD+FIHAKC+  S+LFELAQ+I+K LP++S+E+FEDIY +VY+ GRDLSGFIDGTEN A  + RQE AV   TGGSYVITQKW+HK  VI+ EK+  LEG+VGR R+DSTEL RK+ TSHVARMT G NF   K  EIVRQS PFG+LSGE GLFFIGY+A+ +  +YML+RMVG+  DG  DDIM++S+ VK TYWYFPG  EL  L
Sbjct:    1 VYSEGKNHALYLWINLKPGANSREVAKSVAKIQKYVDQVTDPTMKDDDDEILAGVGFGPNFFSQV--AGKTMKSFVYPHRKGALGDMPSSGGDVFIHAKCNTPSKLFELAQVIIKNLPKDSVENFEDIYSFVYKNGRDLSGFIDGTENRADEEGRQETAVEKETGGSYVITQKWLHKLDVIDGEKDRTLEGWVGRARNDSTELSRKTITSHVARMTSGNNFNAPKPVEIVRQSMPFGSLSGEKGLFFIGYAASPQNFEYMLDRMVGSGGDGQNDDIMRLSQNVKGTYWYFPGASELKKL 297
[Close]
BLAST of Deferrochelatase/peroxidase YfeX vs. TrEMBL
Match: A0A2C9K2E1 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106078669 PE=4 SV=1)

HSP 1 Score: 381.333 bits (978), Expect = 2.039e-126
Identity = 205/395 (51.90%), Postives = 263/395 (66.58%), Query Frame = 3 
Query:   54 TIEMIKNLSLFFRQTFSKQSFGNQQKSFLNQFARNKFTNSNKIILCGGAVTVFG--GIF-----YTLFDRNKLKLT---LNINPAFCASLLKSQESVFSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRXXXXXXXXXXYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTGGPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDL 1208
            T++ +  +     ++FSK++F  Q    LN  +R   +     I+ G A+  F   GIF      T+ D  K K+    LN      A+   SQ SV+S  K HALYL I+L+P  + K+V K  A L+K VD I    ++D+E+EVLAGVGFGPNF  ++         + Y  RKG  G+MPS+GGDIF+HAK ++IS+LFEL+QL +  LP++S+ESFEDIY +VY+  RDLSGFIDGTEN    + RQ +AV   TGGSYVITQKWIH F VIN EK ++LE +VGR+RSDSTEL RK  TSHVARMTGG  F+Q K +EIVRQS PFGT+S E GLFFIGY+A+ +  ++ML+RMVGA  D   DDIMK+S  VK TYWYFPGQ EL+ L
Sbjct:    6 TLKYLNVVQSHLTKSFSKKAFIRQ----LNTSSRPTASQKGGAIILGSALAGFTAYGIFKKLQGQTVLDE-KFKIVDSVLNHATVKAATPPASQPSVWSPGKDHALYLWIYLKPTANSKDVAKVAATLQKMVDTIVDPTMKDEEDEVLAGVGFGPNFYAQVAGKTRKNFVYPY--RKGNLGDMPSSGGDIFVHAKSNQISKLFELSQLFINSLPKDSVESFEDIYSFVYQIQRDLSGFIDGTENRVDDEGRQLIAVEKETGGSYVITQKWIHNFNVINTEKTKILENWVGRERSDSTELPRKPITSHVARMTGGNAFQQPKAYEIVRQSMPFGTVSKEAGLFFIGYAADPKNFEFMLDRMVGAGGDSQCDDIMKLSTNVKGTYWYFPGQSELNKL 393
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BLAST of Deferrochelatase/peroxidase YfeX vs. Planmine SMEST
Match: SMESG000077723.1 (SMESG000077723.1)

HSP 1 Score: 761.525 bits (1965), Expect = 0.000e+0
Identity = 378/384 (98.44%), Postives = 381/384 (99.22%), Query Frame = 3 
Query:   63 MIKNLSLFFRQTFSKQSFGNQQKSFLNQFARNKFTNSNKIILCGGAVTVFGGIFYTLFDRNKLKLTLNINPAFCASLLKSQESVFSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRXXXXXXXXXXYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTGGPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDLLK 1214
            MIKNLSLFFR+TFSKQSFGNQQKSFLNQFARNKFTNSNKIILCGGAVTVFGGIFYT FDRNKLKLTLNINPAFCAS LKSQESV+SQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRPSKPPPSSFSYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTGGPNFEQKK FEIVRQSQPFGTLSGENGLFFIGYSA+VEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDLLK
Sbjct:    1 MIKNLSLFFRKTFSKQSFGNQQKSFLNQFARNKFTNSNKIILCGGAVTVFGGIFYTFFDRNKLKLTLNINPAFCASFLKSQESVYSQTKHHALYLTIHLRPEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRPSKPPPSSFSYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKELPENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGSYVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTGGPNFEQKKNFEIVRQSQPFGTLSGENGLFFIGYSASVEALDYMLNRMVGATEDGLGDDIMKMSKCVKSTYWYFPGQQELDDLLK 384
The following BLAST results are available for this feature:
BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q8XBI9|YFEX_ECO574.349e-3130.32Dye-decolorizing peroxidase YfeX OS=Escherichia co... [more]
sp|P76536|YFEX_ECOLI9.109e-3130.32Dye-decolorizing peroxidase YfeX OS=Escherichia co... [more]
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BLAST of Deferrochelatase/peroxidase YfeX vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3JN882.991e-13458.84uncharacterized protein LOC106174718 OS=Lingula un... [more]
A0A1S3J2Z27.305e-13458.84uncharacterized protein LOC106169733 OS=Lingula un... [more]
A0A194ALU09.186e-13054.87Uncharacterized protein OS=Pinctada fucata OX=5042... [more]
V4C9506.990e-12760.54Uncharacterized protein (Fragment) OS=Lottia gigan... [more]
A0A2C9K2E12.039e-12651.90Uncharacterized protein OS=Biomphalaria glabrata O... [more]
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Deferrochelatase/peroxidase YfeX vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000077723.10.000e+098.44SMESG000077723.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30017015 ID=SMED30017015|Name=Deferrochelatase/peroxidase YfeX|organism=Schmidtea mediterranea sexual|type=transcript|length=1344bp
CCGCCGCATAAAATTATTTATTGTGGGACATTTAAAATATTTTTGAGTTT
TTAACGATTGAAATGATAAAAAACTTGTCTTTGTTTTTTAGGCAAACTTT
TTCAAAACAATCTTTTGGAAATCAGCAAAAGTCATTTCTCAATCAATTTG
CACGTAATAAATTTACAAATTCAAATAAAATAATCTTATGCGGCGGAGCG
GTTACTGTTTTTGGAGGTATTTTTTATACTCTCTTTGATCGAAATAAACT
CAAACTAACACTTAATATCAATCCGGCATTTTGTGCGTCATTACTGAAAT
CTCAAGAATCTGTTTTCTCTCAAACTAAACATCATGCGTTATATTTAACA
ATACACCTAAGGCCAGAAGTTTCTGTTAAAGAAGTTATCAAAAAAGTCGC
CAACTTAAAAAAGTATGTTGATAAAATATCGCCAATTAATCTTAGAGATG
ACGAAAATGAAGTGCTTGCTGGTGTTGGATTTGGTCCGAATTTCCTAACC
AAAATCAGGCCTTCTAAGCCTCCCCCGTCTAGTTTTTCTTATTACACTAG
AAAGGGACAATTCGGAGAAATGCCAAGCACTGGTGGAGATATTTTTATCC
ATGCTAAATGTGATGAGATAAGCAGATTATTTGAATTGGCTCAGCTCATT
CTGAAAGAATTGCCTGAAAATAGCATTGAAAGTTTTGAAGACATTTATGG
ATGGGTTTATCGGTATGGACGTGATTTAAGTGGGTTTATCGACGGTACAG
AAAATCCAGCCAGTACAGATCATCGGCAAGAAGTTGCTGTTAATACGACT
ACAGGAGGGAGTTATGTTATCACACAGAAGTGGATTCATAAATTTGGAGT
TATTAACAAAGAAAAGGAAGAGGTTCTGGAAGGTTATGTTGGACGTAAGC
GCTCTGATAGTACAGAATTGAAGAGAAAATCAACTACGTCCCATGTTGCT
AGAATGACAGGAGGTCCAAACTTTGAACAGAAAAAAAAATTTGAAATTGT
GAGACAATCTCAACCATTTGGTACGTTATCAGGAGAAAATGGTCTATTTT
TCATCGGTTATTCCGCCAATGTAGAAGCTCTGGATTATATGTTAAATAGG
ATGGTAGGTGCAACTGAAGATGGACTTGGTGATGATATAATGAAAATGTC
CAAATGCGTTAAAAGCACATATTGGTATTTCCCAGGACAACAGGAACTGG
ATGATTTGCTTAAATAAATGAAAATATAGCGAATAATTATAGATTCATAA
TCACATTATTGGCTTGTTTTACTAATTTATGTAACTTTTATCACATTTGT
AATTTTGACAAATTTTTTCGATGAAATAAATCATTTGATTTTTT
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protein sequence of SMED30017015-orf-1

>SMED30017015-orf-1 ID=SMED30017015-orf-1|Name=SMED30017015-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=385bp
MIKNLSLFFRQTFSKQSFGNQQKSFLNQFARNKFTNSNKIILCGGAVTVF
GGIFYTLFDRNKLKLTLNINPAFCASLLKSQESVFSQTKHHALYLTIHLR
PEVSVKEVIKKVANLKKYVDKISPINLRDDENEVLAGVGFGPNFLTKIRP
SKPPPSSFSYYTRKGQFGEMPSTGGDIFIHAKCDEISRLFELAQLILKEL
PENSIESFEDIYGWVYRYGRDLSGFIDGTENPASTDHRQEVAVNTTTGGS
YVITQKWIHKFGVINKEKEEVLEGYVGRKRSDSTELKRKSTTSHVARMTG
GPNFEQKKKFEIVRQSQPFGTLSGENGLFFIGYSANVEALDYMLNRMVGA
TEDGLGDDIMKMSKCVKSTYWYFPGQQELDDLLK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR006314Dyp_peroxidase
IPR011008Dimeric_a/b-barrel
Vocabulary: molecular function
TermDefinition
GO:0004601peroxidase activity
GO:0020037heme binding
GO:0015114phosphate ion transmembrane transporter activity
GO:0015321sodium-dependent phosphate transmembrane transporter activity
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
GO:0035435phosphate ion transmembrane transport
GO:0044341sodium-dependent phosphate transport
GO:0098869cellular oxidant detoxification
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006314Dyp-type peroxidaseTIGRFAMTIGR01413TIGR01413coord: 81..378
e-value: 1.2E-37
score: 127.9
IPR006314Dyp-type peroxidasePFAMPF04261Dyp_peroxcoord: 88..378
e-value: 1.1E-36
score: 126.5
IPR006314Dyp-type peroxidasePROSITEPS51404DYP_PEROXIDASEcoord: 68..382
score: 16.861
NoneNo IPR availablePANTHERPTHR30521:SF0DEFERROCHELATASE/PEROXIDASE YFEX-RELATEDcoord: 79..382
NoneNo IPR availablePANTHERPTHR30521UNCHARACTERIZEDcoord: 79..382
NoneNo IPR availableTMHMMTMhelixcoord: 40..58
IPR011008Dimeric alpha-beta barrelSUPERFAMILYSSF54909Dimeric alpha+beta barrelcoord: 80..382