Kunitz-type serine protease inhibitor

Overview


Name	Kunitz-type serine protease inhibitor
Smed ID	SMED30017004
Length (bp)	262

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Kunitz-type serine protease inhibitor (SMED30017004) t-SNE clustered cells

Violin plots show distribution of expression levels for Kunitz-type serine protease inhibitor (SMED30017004) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Kunitz-type serine protease inhibitor (SMED30017004) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Kunitz-type serine protease inhibitor (SMED30017004) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30017004

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pigment cell	SMED30017004	SMESG000037995.1	SmedASXL_008102	SmedAsxl_ww_GCZZ01	PMID:29158443 He et al., 2017
protonephridia	SMED30017004	SMESG000037995.1	dd_Smed_v4_300_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30017004	SMESG000037995.1	dd_Smed_v4_300_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30017004	SMESG000037995.1	dd_Smed_v4_300_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30017004	SMESG000037995.1	dd_Smed_v4_300_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30017004	SMESG000037995.1	dd_Smed_v4_300_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30017004	SMESG000037995.1	dd_Smed_v4_300_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017004	SMESG000037995.1	Contig35179	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30017004	SMESG000037995.1	Contig35179	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
tail region	SMED30017004	SMESG000037995.1	dd_Smed_v6_300_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
goblet cell	SMED30017004	SMESG000037995.1	dd_Smed_v6_300_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 63.929 bits (154), Expect = 4.465e-13
Identity = 26/49 (53.06%), Postives = 36/49 (73.47%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C LPA +G C AY  ++ +NS T +C+ FV+GGC+GN NNF S++ CEE
Sbjct:  293 CSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEE 341

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 63.929 bits (154), Expect = 4.525e-13
Identity = 26/49 (53.06%), Postives = 36/49 (73.47%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C LPA +G C AY  ++ +NS T +C+ FV+GGC+GN NNF S++ CEE
Sbjct:  386 CSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEE 434

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: SPINT3 (serine peptidase inhibitor, Kunitz type 3 [Source:HGNC Symbol;Acc:HGNC:11248])

HSP 1 Score: 60.077 bits (144), Expect = 5.750e-13
Identity = 26/50 (52.00%), Postives = 34/50 (68.00%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            C  P EKG C  Y+ ++FFN  T +CELF +GGC GN NNF  ++ CE+F
Sbjct:   36 CAFPMEKGPCQTYMTRWFFNFETGECELFAYGGCGGNSNNFLRKEKCEKF 85

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: EPPIN (epididymal peptidase inhibitor [Source:HGNC Symbol;Acc:HGNC:15932])

HSP 1 Score: 57.7658 bits (138), Expect = 8.438e-12
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 3

Query:   72 CSVISFGES----RAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C V S G+     +   C +P E G CLAY   ++++   N C +FV+GGCQGN NNF S+ +C
Sbjct:   44 CCVFSCGKKCLDLKQDVCEMPKETGPCLAYFLHWWYDKKDNTCSMFVYGGCQGNNNNFQSKANC 107

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Match: EPPIN (epididymal peptidase inhibitor [Source:HGNC Symbol;Acc:HGNC:15932])

HSP 1 Score: 57.3806 bits (137), Expect = 1.326e-11
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 3

Query:   72 CSVISFGES----RAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C V S G+     +   C +P E G CLAY   ++++   N C +FV+GGCQGN NNF S+ +C
Sbjct:   60 CCVFSCGKKCLDLKQDVCEMPKETGPCLAYFLHWWYDKKDNTCSMFVYGGCQGNNNNFQSKANC 123

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 67.781 bits (164), Expect = 1.002e-14
Identity = 26/53 (49.06%), Postives = 37/53 (69.81%), Query Frame = 3

Query:   96 SRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +RA  C LP E+G C  Y  +F+FN+  N+C+ F +GGC+GN+NNF   +DCE
Sbjct:  534 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCE 586

HSP 2 Score: 62.3882 bits (150), Expect = 8.069e-13
Identity = 29/86 (33.72%), Postives = 44/86 (51.16%), Query Frame = 3

Query:   27 TLIKMNSIIIATLVLCSVISFGESRAPE----------CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            T + ++ +  A  V  SV+  G +  P           C +P + G+C  Y  ++FFNS T +CE F +G C GN+NNF  +  CE
Sbjct: 2139 TGLPVSIVTAAPTVQASVVPLGTTPQPHFEPKFDGRVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCE 2224

HSP 3 Score: 55.4546 bits (132), Expect = 2.082e-10
Identity = 20/49 (40.82%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +C  P E G C  + ++++FN     C  F +GGC GN+N+F +QK+CE
Sbjct: 1082 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECE 1130

HSP 4 Score: 53.5286 bits (127), Expect = 8.249e-10
Identity = 20/52 (38.46%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  105 PECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            P C  P  KG C     +++++  T+ C LF + GC GN NNF S +DC+  
Sbjct: 2740 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRL 2791

HSP 5 Score: 51.6026 bits (122), Expect = 4.053e-9
Identity = 18/47 (38.30%), Postives = 28/47 (59.57%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C  P + G C     ++FF+     C++F + GCQGN NNF S+++C
Sbjct:  737 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEEC 783

HSP 6 Score: 51.2174 bits (121), Expect = 6.291e-9
Identity = 19/50 (38.00%), Postives = 30/50 (60.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            EC   +  G C    +++F+N  + KCE F + GC GN NN+ S++ CE+
Sbjct:  422 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACED 471

HSP 7 Score: 49.2914 bits (116), Expect = 3.185e-8
Identity = 23/68 (33.82%), Postives = 34/68 (50.00%), Query Frame = 3

Query:   57 ATLVLCS---VISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            AT+  C    V++  +S    C    E G C     +F F+ + N C  F +GGC GN NNF + ++C
Sbjct:  845 ATMQECRSRCVMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQEC 912

HSP 8 Score: 43.1282 bits (100), Expect = 4.528e-6
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3

Query:  156 KFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            ++F++   N+C+LF F GC GN N F  + DCE
Sbjct: 3093 QYFYSPRDNRCKLFFFRGCGGNLNRFERKSDCE 3125

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3d.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 67.781 bits (164), Expect = 1.012e-14
Identity = 26/53 (49.06%), Postives = 37/53 (69.81%), Query Frame = 3

Query:   96 SRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +RA  C LP E+G C  Y  +F+FN+  N+C+ F +GGC+GN+NNF   +DCE
Sbjct:  534 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCE 586

HSP 2 Score: 62.3882 bits (150), Expect = 8.228e-13
Identity = 29/86 (33.72%), Postives = 44/86 (51.16%), Query Frame = 3

Query:   27 TLIKMNSIIIATLVLCSVISFGESRAPE----------CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            T + ++ +  A  V  SV+  G +  P           C +P + G+C  Y  ++FFNS T +CE F +G C GN+NNF  +  CE
Sbjct: 2130 TGLPVSIVTAAPTVQASVVPLGTTPQPHFEPKFDGRVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCE 2215

HSP 3 Score: 55.4546 bits (132), Expect = 2.082e-10
Identity = 20/49 (40.82%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +C  P E G C  + ++++FN     C  F +GGC GN+N+F +QK+CE
Sbjct: 1073 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECE 1121

HSP 4 Score: 53.5286 bits (127), Expect = 8.411e-10
Identity = 20/52 (38.46%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  105 PECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            P C  P  KG C     +++++  T+ C LF + GC GN NNF S +DC+  
Sbjct: 2731 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRL 2782

HSP 5 Score: 51.6026 bits (122), Expect = 4.093e-9
Identity = 18/47 (38.30%), Postives = 28/47 (59.57%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C  P + G C     ++FF+     C++F + GCQGN NNF S+++C
Sbjct:  728 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEEC 774

HSP 6 Score: 51.2174 bits (121), Expect = 6.291e-9
Identity = 19/50 (38.00%), Postives = 30/50 (60.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            EC   +  G C    +++F+N  + KCE F + GC GN NN+ S++ CE+
Sbjct:  422 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACED 471

HSP 7 Score: 49.2914 bits (116), Expect = 3.154e-8
Identity = 23/68 (33.82%), Postives = 34/68 (50.00%), Query Frame = 3

Query:   57 ATLVLCS---VISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            AT+  C    V++  +S    C    E G C     +F F+ + N C  F +GGC GN NNF + ++C
Sbjct:  836 ATMQECRSRCVMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQEC 903

HSP 8 Score: 43.1282 bits (100), Expect = 4.528e-6
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3

Query:  156 KFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            ++F++   N+C+LF F GC GN N F  + DCE
Sbjct: 3084 QYFYSPRDNRCKLFFFRGCGGNLNRFERKSDCE 3116

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 67.3958 bits (163), Expect = 1.104e-14
Identity = 26/53 (49.06%), Postives = 37/53 (69.81%), Query Frame = 3

Query:   96 SRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +RA  C LP E+G C  Y  +F+FN+  N+C+ F +GGC+GN+NNF   +DCE
Sbjct:  405 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCE 457

HSP 2 Score: 62.003 bits (149), Expect = 9.067e-13
Identity = 29/86 (33.72%), Postives = 44/86 (51.16%), Query Frame = 3

Query:   27 TLIKMNSIIIATLVLCSVISFGESRAPE----------CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            T + ++ +  A  V  SV+  G +  P           C +P + G+C  Y  ++FFNS T +CE F +G C GN+NNF  +  CE
Sbjct: 2010 TGLPVSIVTAAPTVQASVVPLGTTPQPHFEPKFDGRVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCE 2095

HSP 3 Score: 55.4546 bits (132), Expect = 2.250e-10
Identity = 20/49 (40.82%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +C  P E G C  + ++++FN     C  F +GGC GN+N+F +QK+CE
Sbjct:  953 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECE 1001

HSP 4 Score: 53.5286 bits (127), Expect = 9.091e-10
Identity = 20/52 (38.46%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  105 PECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            P C  P  KG C     +++++  T+ C LF + GC GN NNF S +DC+  
Sbjct: 2611 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRL 2662

HSP 5 Score: 51.6026 bits (122), Expect = 4.381e-9
Identity = 18/47 (38.30%), Postives = 28/47 (59.57%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C  P + G C     ++FF+     C++F + GCQGN NNF S+++C
Sbjct:  608 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEEC 654

HSP 6 Score: 51.2174 bits (121), Expect = 6.668e-9
Identity = 19/50 (38.00%), Postives = 30/50 (60.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            EC   +  G C    +++F+N  + KCE F + GC GN NN+ S++ CE+
Sbjct:  293 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACED 342

HSP 7 Score: 49.2914 bits (116), Expect = 3.476e-8
Identity = 23/68 (33.82%), Postives = 34/68 (50.00%), Query Frame = 3

Query:   57 ATLVLCS---VISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            AT+  C    V++  +S    C    E G C     +F F+ + N C  F +GGC GN NNF + ++C
Sbjct:  716 ATMQECRSRCVMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQEC 783

HSP 8 Score: 43.1282 bits (100), Expect = 4.847e-6
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3

Query:  156 KFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            ++F++   N+C+LF F GC GN N F  + DCE
Sbjct: 2964 QYFYSPRDNRCKLFFFRGCGGNLNRFERKSDCE 2996

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3h.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 67.0106 bits (162), Expect = 1.848e-14
Identity = 26/53 (49.06%), Postives = 37/53 (69.81%), Query Frame = 3

Query:   96 SRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +RA  C LP E+G C  Y  +F+FN+  N+C+ F +GGC+GN+NNF   +DCE
Sbjct:  313 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCE 365

HSP 2 Score: 61.6178 bits (148), Expect = 1.547e-12
Identity = 29/86 (33.72%), Postives = 44/86 (51.16%), Query Frame = 3

Query:   27 TLIKMNSIIIATLVLCSVISFGESRAPE----------CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            T + ++ +  A  V  SV+  G +  P           C +P + G+C  Y  ++FFNS T +CE F +G C GN+NNF  +  CE
Sbjct: 1537 TGLPVSIVTAAPTVQASVVPLGTTPQPHFEPKFDGRVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCE 1622

HSP 3 Score: 54.6842 bits (130), Expect = 3.350e-10
Identity = 20/49 (40.82%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +C  P E G C  + ++++FN     C  F +GGC GN+N+F +QK+CE
Sbjct:  861 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECE 909

HSP 4 Score: 53.1434 bits (126), Expect = 1.289e-9
Identity = 20/52 (38.46%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  105 PECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            P C  P  KG C     +++++  T+ C LF + GC GN NNF S +DC+  
Sbjct: 2138 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRL 2189

HSP 5 Score: 51.2174 bits (121), Expect = 6.398e-9
Identity = 18/47 (38.30%), Postives = 28/47 (59.57%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C  P + G C     ++FF+     C++F + GCQGN NNF S+++C
Sbjct:  516 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEEC 562

HSP 6 Score: 50.447 bits (119), Expect = 1.003e-8
Identity = 19/50 (38.00%), Postives = 30/50 (60.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            EC   +  G C    +++F+N  + KCE F + GC GN NN+ S++ CE+
Sbjct:  201 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACED 250

HSP 7 Score: 48.521 bits (114), Expect = 5.178e-8
Identity = 23/68 (33.82%), Postives = 34/68 (50.00%), Query Frame = 3

Query:   57 ATLVLCS---VISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            AT+  C    V++  +S    C    E G C     +F F+ + N C  F +GGC GN NNF + ++C
Sbjct:  624 ATMQECRSRCVMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQEC 691

HSP 8 Score: 42.743 bits (99), Expect = 6.549e-6
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3

Query:  156 KFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            ++F++   N+C+LF F GC GN N F  + DCE
Sbjct: 2491 QYFYSPRDNRCKLFFFRGCGGNLNRFERKSDCE 2523

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 66.6254 bits (161), Expect = 2.058e-14
Identity = 26/53 (49.06%), Postives = 37/53 (69.81%), Query Frame = 3

Query:   96 SRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +RA  C LP E+G C  Y  +F+FN+  N+C+ F +GGC+GN+NNF   +DCE
Sbjct:  534 ARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCE 586

HSP 2 Score: 61.2326 bits (147), Expect = 1.690e-12
Identity = 29/86 (33.72%), Postives = 44/86 (51.16%), Query Frame = 3

Query:   27 TLIKMNSIIIATLVLCSVISFGESRAPE----------CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            T + ++ +  A  V  SV+  G +  P           C +P + G+C  Y  ++FFNS T +CE F +G C GN+NNF  +  CE
Sbjct: 1613 TGLPVSIVTAAPTVQASVVPLGTTPQPHFEPKFDGRVVCAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCE 1698

HSP 3 Score: 54.6842 bits (130), Expect = 3.519e-10
Identity = 20/49 (40.82%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +C  P E G C  + ++++FN     C  F +GGC GN+N+F +QK+CE
Sbjct: 1082 KCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECE 1130

HSP 4 Score: 53.1434 bits (126), Expect = 1.436e-9
Identity = 20/52 (38.46%), Postives = 29/52 (55.77%), Query Frame = 3

Query:  105 PECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            P C  P  KG C     +++++  T+ C LF + GC GN NNF S +DC+  
Sbjct: 2214 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRL 2265

HSP 5 Score: 51.2174 bits (121), Expect = 6.854e-9
Identity = 18/47 (38.30%), Postives = 28/47 (59.57%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C  P + G C     ++FF+     C++F + GCQGN NNF S+++C
Sbjct:  737 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEEC 783

HSP 6 Score: 50.447 bits (119), Expect = 1.053e-8
Identity = 19/50 (38.00%), Postives = 30/50 (60.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            EC   +  G C    +++F+N  + KCE F + GC GN NN+ S++ CE+
Sbjct:  422 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACED 471

HSP 7 Score: 48.521 bits (114), Expect = 5.602e-8
Identity = 23/68 (33.82%), Postives = 34/68 (50.00%), Query Frame = 3

Query:   57 ATLVLCS---VISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            AT+  C    V++  +S    C    E G C     +F F+ + N C  F +GGC GN NNF + ++C
Sbjct:  845 ATMQECRSRCVMAMKKSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQEC 912

HSP 8 Score: 42.743 bits (99), Expect = 6.948e-6
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3

Query:  156 KFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            ++F++   N+C+LF F GC GN N F  + DCE
Sbjct: 2567 QYFYSPRDNRCKLFFFRGCGGNLNRFERKSDCE 2599

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: CG13748 (gene:FBgn0033355 transcript:FBtr0088656)

HSP 1 Score: 61.2326 bits (147), Expect = 1.238e-13
Identity = 32/80 (40.00%), Postives = 42/80 (52.50%), Query Frame = 3

Query:   18 FMFTLIKMNSIIIATLVLCSVISFGESRAPE--CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            F   L    S     + L  V + G +  PE  C +PA KG+C A I ++ ++    KC  F FGGC GN+NNF S KDC
Sbjct:   27 FPMDLYDDVSDFFDAISLDDVANTGRNTHPEQFCLMPARKGVCRALIPRWRYDPEQKKCVEFKFGGCDGNENNFASYKDC 106

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0077545)

HSP 1 Score: 59.3066 bits (142), Expect = 1.310e-12
Identity = 24/50 (48.00%), Postives = 32/50 (64.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            +C  P E G C A   ++ +N  T  CE FV+GGC GNKNNF S++ CE+
Sbjct:   54 DCHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQ 103

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0346581)

HSP 1 Score: 59.3066 bits (142), Expect = 1.310e-12
Identity = 24/50 (48.00%), Postives = 32/50 (64.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            +C  P E G C A   ++ +N  T  CE FV+GGC GNKNNF S++ CE+
Sbjct:   54 DCHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQ 103

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: CG10031 (gene:FBgn0031563 transcript:FBtr0077544)

HSP 1 Score: 54.6842 bits (130), Expect = 6.363e-11
Identity = 20/51 (39.22%), Postives = 32/51 (62.75%), Query Frame = 3

Query:  105 PECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            P+C  P + G C   +E+F++N  +  CE F +GGC+GN N +  ++ CEE
Sbjct:   73 PKCLQPLDVGPCRMSLERFYYNKDSKACETFKYGGCRGNDNRWGFRQTCEE 123

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Match: CG2816 (gene:FBgn0031564 transcript:FBtr0300299)

HSP 1 Score: 51.9878 bits (123), Expect = 3.269e-10
Identity = 20/52 (38.46%), Postives = 29/52 (55.77%), Query Frame = 3

Query:   99 RAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            R   C  P   G C  Y+E + +N     CE F++GGC GN N F+++ +CE
Sbjct:   27 RVKLCLQPMISGRCFGYVESYAYNPIKRHCEPFIYGGCGGNDNRFSTKAECE 78

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: wfikkn1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:NCBI gene;Acc:406570])

HSP 1 Score: 61.6178 bits (148), Expect = 2.205e-12
Identity = 22/48 (45.83%), Postives = 36/48 (75.00%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            C LPA +G C  +  ++F+NS T +CE F++GGC GNKN+F ++++C+
Sbjct:  257 CSLPAVQGPCRHWQARWFYNSLTERCEAFLYGGCSGNKNSFGTRRECD 304

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: wfikkn2a (info WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2a [Source:ZFIN;Acc:ZDB-GENE-120914-1])

HSP 1 Score: 60.8474 bits (146), Expect = 3.813e-12
Identity = 27/60 (45.00%), Postives = 40/60 (66.67%), Query Frame = 3

Query:   78 VISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            ++S G   A  C LP+ +G C AY  ++ ++ A  KC+ FV+GGC GN+NNF S++ CEE
Sbjct:  372 MLSCGAELAAPCSLPSVQGPCKAYKPRWAYSHALKKCQSFVYGGCGGNENNFESKEACEE 431

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: wfikkn1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:NCBI gene;Acc:406570])

HSP 1 Score: 60.077 bits (144), Expect = 7.352e-12
Identity = 22/48 (45.83%), Postives = 36/48 (75.00%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            C LPA +G C  +  ++F+NS T +CE F++GGC GNKN+F ++++C+
Sbjct:  328 CSLPAVQGPCRHWQARWFYNSLTERCEAFLYGGCSGNKNSFGTRRECD 375

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: wfikkn2b (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2b [Source:ZFIN;Acc:ZDB-GENE-110414-1])

HSP 1 Score: 58.5362 bits (140), Expect = 2.529e-11
Identity = 22/52 (42.31%), Postives = 36/52 (69.23%), Query Frame = 3

Query:  102 APECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            +  C LP  KG C AY  ++ ++S  ++C LF++GGC+ N+NNF S++ CE+
Sbjct:  372 SASCMLPIHKGPCKAYELRWAYSSTLHQCRLFIYGGCKANENNFKSKRSCED 423

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Match: tfpia (tissue factor pathway inhibitor a [Source:ZFIN;Acc:ZDB-GENE-030711-1])

HSP 1 Score: 57.3806 bits (137), Expect = 5.712e-11
Identity = 24/50 (48.00%), Postives = 34/50 (68.00%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            C L  ++G C A  ++F+F+  T +CE F +GGCQGN NNF + +DCEE 
Sbjct:   42 CALKKDEGPCKAMKDRFYFDIDTGRCEPFEYGGCQGNANNFETLQDCEEM 91

HSP 2 Score: 48.521 bits (114), Expect = 7.259e-8
Identity = 21/55 (38.18%), Postives = 35/55 (63.64%), Query Frame = 3

Query:   93 ESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            E+++P C L  E G C   + ++FF+  + +C+ F +GGC GN NNF + K C++
Sbjct:   96 ENKSP-CHLEDEPGPCRGLVPRYFFDQKSQECKQFFYGGCFGNANNFKTIKACQQ 149

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:NCBI gene;Acc:548483])

HSP 1 Score: 57.3806 bits (137), Expect = 4.329e-11
Identity = 25/74 (33.78%), Postives = 44/74 (59.46%), Query Frame = 3

Query:   39 MNSIIIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            + +++ A+L++    S  +     C LP ++G C A I  ++++  T  C+ F++GGC GN N F S +DCE+F
Sbjct:    5 LQALMAASLLIQVFGSPMQGNTTVCLLPPDEGPCKALIPHYYYDRYTQTCQEFLYGGCDGNSNKFLSMEDCEKF 78

HSP 2 Score: 50.8322 bits (120), Expect = 1.175e-8
Identity = 20/48 (41.67%), Postives = 31/48 (64.58%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            C+ P ++G C A + +++FN  +  CE FV+ GC GN NNF   +DC+
Sbjct:  149 CYSPKDEGSCSASVTRYYFNIESKACEEFVYTGCGGNSNNFVKMEDCD 196

HSP 3 Score: 50.8322 bits (120), Expect = 1.355e-8
Identity = 24/82 (29.27%), Postives = 43/82 (52.44%), Query Frame = 3

Query:   18 FMFTLIKMNSIIIATLVLCSVISFGESRAPE-CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            F++     NS    ++  C    +   + P+ C +  ++G C  +++++ +N  T KCE F +GGC GN NNF  +  C +F
Sbjct:   57 FLYGGCDGNSNKFLSMEDCEKFCWKIKKVPKACRMDPDEGPCRGFLKRYAYNMQTMKCEQFYYGGCYGNDNNFKDKASCMDF 138

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:Xenbase;Acc:XB-GENE-976485])

HSP 1 Score: 56.9954 bits (136), Expect = 1.489e-10
Identity = 22/56 (39.29%), Postives = 37/56 (66.07%), Query Frame = 3

Query:   93 ESRAPE-CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            ++  PE C L  E+G C  ++ K++++  T  C  F +GGC GN+N F++QK+CE+
Sbjct: 3002 QTTGPEVCNLQKEEGTCRDFVLKWYYDPETKSCARFWYGGCGGNENRFSTQKECEK 3057

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: cmtr2 (cap methyltransferase 2 [Source:Xenbase;Acc:XB-GENE-6035233])

HSP 1 Score: 52.7582 bits (125), Expect = 1.966e-10
Identity = 22/50 (44.00%), Postives = 31/50 (62.00%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            C LP   G C A    + F++A  KC  F +GGCQGN N F ++K+C+E+
Sbjct:    6 CRLPITPGPCKAAKTHWAFDAAQGKCVTFAYGGCQGNGNQFYTEKECKEY 55

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: cmtr2 (cap methyltransferase 2 [Source:Xenbase;Acc:XB-GENE-6035233])

HSP 1 Score: 52.373 bits (124), Expect = 2.393e-10
Identity = 22/50 (44.00%), Postives = 31/50 (62.00%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            C LP   G C A    + F++A  KC  F +GGCQGN N F ++K+C+E+
Sbjct:    6 CRLPITPGPCKAAKTHWAFDAAQGKCVTFAYGGCQGNGNQFYTEKECKEY 55

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:Xenbase;Acc:XB-GENE-976485])

HSP 1 Score: 55.8398 bits (133), Expect = 3.065e-10
Identity = 22/53 (41.51%), Postives = 35/53 (66.04%), Query Frame = 3

Query:  102 APE-CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
             PE C L  E+G C  ++ K++++  T  C  F +GGC GN+N F++QK+CE+
Sbjct: 2825 GPEVCNLQKEEGTCRDFVLKWYYDPETKSCARFWYGGCGGNENRFSTQKECEK 2877

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Spint3 (serine peptidase inhibitor, Kunitz type, 3 [Source:MGI Symbol;Acc:MGI:3651470])

HSP 1 Score: 60.077 bits (144), Expect = 2.841e-13
Identity = 25/54 (46.30%), Postives = 36/54 (66.67%), Query Frame = 3

Query:   93 ESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            +S  P C LP + G C A   ++++NS T KCE F +GGC+GN+NNF S+  C+
Sbjct:   29 KSLPPMCILPKDIGGCRAVFVRWYYNSKTGKCEWFRYGGCKGNENNFPSRSQCQ 82

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Wfikkn2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:MGI Symbol;Acc:MGI:2669209])

HSP 1 Score: 63.929 bits (154), Expect = 3.559e-13
Identity = 26/49 (53.06%), Postives = 36/49 (73.47%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C LPA +G C AY+ ++ +NS T  C+ FV+GGC+GN NNF S++ CEE
Sbjct:  381 CSLPALQGPCKAYVPRWAYNSQTGLCQSFVYGGCEGNGNNFESREACEE 429

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Eppin (epididymal peptidase inhibitor [Source:MGI Symbol;Acc:MGI:1922776])

HSP 1 Score: 60.8474 bits (146), Expect = 4.479e-13
Identity = 23/48 (47.92%), Postives = 34/48 (70.83%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            C LP + G C+AY  +++FN   + C++F++GGCQGN NNF SQ  C+
Sbjct:   77 CSLPKDSGYCMAYFRRWWFNKENSTCQVFIYGGCQGNNNNFQSQSICQ 124

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Match: Wfdc6a (WAP four-disulfide core domain 6A [Source:MGI Symbol;Acc:MGI:2684968])

HSP 1 Score: 59.6918 bits (143), Expect = 1.626e-12
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3

Query:   99 RAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            R   C LP + G CLAY+ ++++N  T+ C  F++GGCQGN NNF S+  C
Sbjct:   73 RQDVCSLPQDPGPCLAYLPRWWYNQETDLCTEFIYGGCQGNPNNFPSEGIC 123

[Close]

Query:   99 RAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            R   C LP + G CLAY+ ++++N  T+ C  F++GGCQGN NNF S+  C
Sbjct:   73 RQDVCSLPQDPGPCLAYLPRWWYNQETDLCTEFIYGGCQGNPNNFPSEGIC 123

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|P00992|VKT3_VIPAA (Kunitz-type serine protease inhibitor 3 OS=Vipera ammodytes ammodytes OX=8705 PE=1 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 3.156e-16
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C+LPA+ G CLAY+  F+++SA+NKC+ F++GGC+GN NNF +  +C  
Sbjct:   31 CYLPADPGRCLAYMPSFYYDSASNKCKKFIYGGCRGNANNFKTWDECRH 79

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DJ50|VKT31_MESMA (Kunitz-type serine protease inhibitor BmKTT-2 OS=Mesobuthus martensii OX=34649 PE=2 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 1.469e-15
Identity = 27/71 (38.03%), Postives = 45/71 (63.38%), Query Frame = 3

Query:   39 MNSIIIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            MN I +  ++L  V +  ++   +C LP++ G C AY  ++F+N    +C+ FV+GGC+GN NNF ++ DC
Sbjct:    2 MNVITVVGIILSVVCTISDAEGVDCTLPSDTGRCKAYFIRYFYNQKAGECQKFVYGGCEGNSNNFLTKSDC 72

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|E5AJX3|VKT_VIPBN (Kunitz-type serine protease inhibitor ki-VN OS=Vipera berus nikolskii OX=1808362 PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 2.191e-15
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C+LPAE G C AY+  F+++SA+NKC+ F++GGC+GN NNF ++ +C  
Sbjct:   31 CYLPAEPGECNAYMPSFYYDSASNKCKKFIYGGCRGNANNFKTRDECHH 79

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|A8Y7N7|VKTC4_DABSI (Kunitz-type serine protease inhibitor C4 OS=Daboia siamensis OX=343250 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 4.471e-15
Identity = 25/56 (44.64%), Postives = 38/56 (67.86%), Query Frame = 3

Query:   90 GESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            G  R   C+LPA+ G C+AYI  F+++S + KC+ F++GGC GN NNF ++  C +
Sbjct:   24 GHDRPKFCYLPADPGECMAYIRSFYYDSESKKCKEFIYGGCHGNANNFPTRDKCRQ 79

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Match: sp|P00991|VKT1_VIPAA (Kunitz-type serine protease inhibitor 1 OS=Vipera ammodytes ammodytes OX=8705 PE=1 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 4.471e-15
Identity = 25/56 (44.64%), Postives = 39/56 (69.64%), Query Frame = 3

Query:   90 GESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            G+     C+LPA+ G C A+I +F+++SA+NKC  F++GGC GN NNF +  +C +
Sbjct:   24 GQDHPKFCYLPADPGRCKAHIPRFYYDSASNKCNKFIYGGCPGNANNFKTWDECRQ 79

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: A0A016W7U9 (BPTI/Kunitz inhibitor domain-containing protein (Fragment) OS=Ancylostoma ceylanicum OX=53326 GN=Acey_s0999.g3344 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.610e-14
Identity = 28/60 (46.67%), Postives = 41/60 (68.33%), Query Frame = 3

Query:   78 VISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            V+S    R   CFLP  KG C A +E++F+N+ TN+C LF +GGC+GN+NNF+ + +C  
Sbjct:    3 VVSAKRPRNETCFLPPVKGRCRASLERWFYNATTNRCHLFRYGGCRGNENNFSDKHECRR 62

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: A0A1S5QJJ2 (Kunitz/BPTI inhibitor-1 OS=Vipera ammodytes ammodytes OX=8705 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.007e-14
Identity = 26/49 (53.06%), Postives = 38/49 (77.55%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C+LPA+ G CLAYI  F+++SA+NKC+ F++GGC+GN NNF +  +C  
Sbjct:   31 CYLPADPGRCLAYIPSFYYDSASNKCKKFIYGGCRGNANNFKTWDECRH 79

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: A0A1S5QK73 (Kunitz/BPTI inhibitor-5 OS=Vipera ammodytes ammodytes OX=8705 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 4.653e-14
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C+LPA+ G CLAY+ +F++N A+NKC+ F++GGC+GN NNF +  +C  
Sbjct:   31 CYLPADPGRCLAYMPRFYYNPASNKCKEFIYGGCRGNANNFKTWDECRH 79

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: A0A1S5QKN1 (Kunitz/BPTI inhibitor-7 OS=Vipera ammodytes ammodytes OX=8705 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.435e-14
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C+LPA+ G CLAY+ +F++N A+NKC+ F++GGC+GN NNF +  +C  
Sbjct:   31 CYLPADPGRCLAYMPRFYYNPASNKCKEFIYGGCRGNANNFKTWDECRH 79

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Match: A0A1S5QJJ1 (Kunitz/BPTI inhibitor-3 OS=Vipera ammodytes ammodytes OX=8705 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.649e-14
Identity = 25/49 (51.02%), Postives = 38/49 (77.55%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C+LPA+ G CLAY+ +F++N A+NKC+ F++GGC+GN NNF +  +C  
Sbjct:   31 CYLPADPGRCLAYMPRFYYNPASNKCKEFIYGGCRGNANNFKTWDECRH 79

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: ENSAMXT00000039940.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000999.1:23065:40314:1 gene:ENSAMXG00000037874.1 transcript:ENSAMXT00000039940.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 64.6994 bits (156), Expect = 1.349e-13
Identity = 23/49 (46.94%), Postives = 36/49 (73.47%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C LP E+G C+ ++ K+F++S +N C  F +GGC GN N FN+Q++CE+
Sbjct:  438 CKLPKEEGTCIKFVLKWFYDSVSNSCTRFWYGGCGGNLNRFNTQEECEK 486

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: ENSAMXT00000051114.1 (pep primary_assembly:Astyanax_mexicanus-2.0:11:5183832:5196079:-1 gene:ENSAMXG00000033686.1 transcript:ENSAMXT00000051114.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.3882 bits (150), Expect = 2.718e-13
Identity = 26/66 (39.39%), Postives = 44/66 (66.67%), Query Frame = 3

Query:   60 TLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            +L +C   S+G+     C L  ++G C AY+ K+F+++  N+C  F +GGC+GN N F+SQ++CE+
Sbjct:  109 SLQVCRWHSYGDVHV--CLLKNDQGPCRAYLLKWFYDTQQNECARFWYGGCKGNGNRFDSQEECED 172

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:NCBI gene;Acc:103023332])

HSP 1 Score: 61.2326 bits (147), Expect = 1.046e-12
Identity = 28/68 (41.18%), Postives = 41/68 (60.29%), Query Frame = 3

Query:   60 TLVLCSVISFGESRAPE-CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            T   C  + +   + P+ C  P+E G C A + ++FFN  T +CE F +GGCQGN+NNF S + C E+
Sbjct:   69 TFQECHKMCYTIPKIPQICRFPSEVGPCRALLSRYFFNMTTMQCEQFYYGGCQGNENNFQSHQSCMEY 136

HSP 2 Score: 53.9138 bits (128), Expect = 5.425e-10
Identity = 21/50 (42.00%), Postives = 33/50 (66.00%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            C LPA +G C   + ++F+N+ T +CE F +GGC GN NNF + ++C + 
Sbjct:   27 CLLPAVEGPCRGLVSRYFYNTLTQRCEEFSYGGCGGNANNFVTFQECHKM 76

HSP 3 Score: 46.9802 bits (110), Expect = 2.310e-7
Identity = 19/42 (45.24%), Postives = 28/42 (66.67%), Query Frame = 3

Query:  126 EKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            +KG C A I ++++NSA   CE F + GC G+ NNF S++ C
Sbjct:  152 DKGKCSASIPRYYYNSAKKTCEEFTYTGCGGSSNNFVSKQSC 193

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: wfikkn2a (WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2-like [Source:NCBI gene;Acc:103032076])

HSP 1 Score: 58.9214 bits (141), Expect = 1.799e-11
Identity = 26/60 (43.33%), Postives = 39/60 (65.00%), Query Frame = 3

Query:   78 VISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            ++S G   A  C LP+ +G C AY  ++ +N    +C+ FV+GGC GN+NNF S++ CEE
Sbjct:  366 MLSCGAELAAPCSLPSLQGPCKAYEPRWAYNHLLKQCQSFVYGGCGGNENNFESKEACEE 425

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Match: ambp (alpha-1-microglobulin/bikunin precursor [Source:NCBI gene;Acc:103022541])

HSP 1 Score: 56.9954 bits (136), Expect = 6.518e-11
Identity = 21/50 (42.00%), Postives = 33/50 (66.00%), Query Frame = 3

Query:  102 APECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            A  C    + G C   I+++++NS+   C+ FVFGGC GN+NNF ++K+C
Sbjct:  223 AESCTAEPDSGPCFGMIQRYYYNSSLMACQQFVFGGCMGNQNNFVTEKEC 272

HSP 2 Score: 53.5286 bits (127), Expect = 1.338e-9
Identity = 23/50 (46.00%), Postives = 32/50 (64.00%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            C LP + G C    E + F+S+  KC  F +GGC+GN N F +QK+CEE+
Sbjct:  282 CRLPMDAGPCNTPQELWAFDSSIGKCVSFKYGGCKGNGNKFYTQKECEEY 331

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:ZFIN;Acc:ZDB-GENE-060503-38])

HSP 1 Score: 56.9954 bits (136), Expect = 1.087e-11
Identity = 23/54 (42.59%), Postives = 35/54 (64.81%), Query Frame = 3

Query:   93 ESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            E +   C LPA+ G C A   ++FFN +T +CE F++GGC  N+NNF + ++C 
Sbjct:   21 EDQRVTCTLPAKPGDCRAAFPRYFFNRSTLRCESFIYGGCHSNENNFETMQECR 74

HSP 2 Score: 46.595 bits (109), Expect = 6.376e-8
Identity = 20/47 (42.55%), Postives = 26/47 (55.32%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C   AE G C A+I ++F+N     C  FV+GGC GN N F   + C
Sbjct:   87 CRPDAETGRCRAHITRYFYNMTLGMCMPFVYGGCGGNNNRFEDMESC 133

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.4546 bits (132), Expect = 3.747e-11
Identity = 22/47 (46.81%), Postives = 31/47 (65.96%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C + A+ G C A I +F +N  T+ CE F +GGC GN NNF+S++ C
Sbjct:  167 CLMKADPGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSC 213

HSP 2 Score: 50.0618 bits (118), Expect = 3.226e-9
Identity = 24/54 (44.44%), Postives = 32/54 (59.26%), Query Frame = 3

Query:  102 APE-CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            AP+ C L  E G+C A + ++FFN  +  CE F +GGCQGN NNF     C + 
Sbjct:   71 APKACRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQH 124

HSP 3 Score: 49.6766 bits (117), Expect = 3.681e-9
Identity = 21/47 (44.68%), Postives = 29/47 (61.70%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C +  + G C AY+ + F+N  T +CE F +GGC GN NNF S  +C
Sbjct:    3 CMMDLDPGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAEC 49

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.4546 bits (132), Expect = 3.754e-11
Identity = 22/47 (46.81%), Postives = 31/47 (65.96%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C + A+ G C A I +F +N  T+ CE F +GGC GN NNF+S++ C
Sbjct:  166 CLMKADPGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSC 212

HSP 2 Score: 49.6766 bits (117), Expect = 3.771e-9
Identity = 21/47 (44.68%), Postives = 29/47 (61.70%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C +  + G C AY+ + F+N  T +CE F +GGC GN NNF S  +C
Sbjct:    3 CMMDLDPGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAEC 49

HSP 3 Score: 49.6766 bits (117), Expect = 3.967e-9
Identity = 24/54 (44.44%), Postives = 32/54 (59.26%), Query Frame = 3

Query:  102 APE-CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            AP+ C L  E G+C A + ++FFN  +  CE F +GGCQGN NNF     C + 
Sbjct:   70 APKACRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQH 123

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 54.6842 bits (130), Expect = 1.078e-10
Identity = 20/49 (40.82%), Postives = 34/49 (69.39%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C LP ++G C  +  ++ +N    +C+ F++GGC+GN+NNF SQ+ CE+
Sbjct:  374 CSLPVQQGPCKQWALRWAYNPLIKRCQSFIYGGCEGNENNFQSQELCED 422

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 53.1434 bits (126), Expect = 3.629e-10
Identity = 20/49 (40.82%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C LP  +G C  +   + +N    +C+L V+GGC+GN+NNF S++ CE+
Sbjct:  399 CHLPPVQGPCRDWERHWAYNPLIKQCQLLVYGGCEGNENNFESREACED 447

HSP 2 Score: 48.9062 bits (115), Expect = 1.283e-8
Identity = 19/59 (32.20%), Postives = 34/59 (57.63%), Query Frame = 3

Query:   84 SFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            +  E  A EC  P +   C +   K++++S  N C+ FV+GGC  N+N F++ + C+ +
Sbjct:  332 AIAEFPASECHEPPDVTHCGSARTKWYYDSGLNACDTFVYGGCDSNRNRFDTYEGCKRW 390

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 62.7734 bits (151), Expect = 3.358e-15
Identity = 26/50 (52.00%), Postives = 35/50 (70.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            EC  P   G C AY E++F+N  + KC+ FV+GGCQGN NNF S+ +CE+
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEK 50

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO26263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 62.7734 bits (151), Expect = 3.358e-15
Identity = 26/50 (52.00%), Postives = 35/50 (70.00%), Query Frame = 3

Query:  108 ECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            EC  P   G C AY E++F+N  + KC+ FV+GGCQGN NNF S+ +CE+
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEK 50

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO47795 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RL41])

HSP 1 Score: 58.9214 bits (141), Expect = 1.434e-13
Identity = 21/49 (42.86%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C LP   G C AY+ ++++N+   +C+ FV+GGC+GN N F S + CE 
Sbjct:    2 CQLPKLPGPCSAYVPRWYYNAKHRRCQKFVYGGCRGNANRFKSHRSCER 50

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO41922 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S322])

HSP 1 Score: 58.5362 bits (140), Expect = 1.948e-13
Identity = 24/49 (48.98%), Postives = 34/49 (69.39%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C LPA  G C+AYI  F+++    KC+ F++GGC GN N F++Q+ CEE
Sbjct:    9 CSLPAVTGRCMAYIPSFYYDMKEEKCKEFIYGGCNGNLNRFDTQEKCEE 57

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Match: EDO38284 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDB9])

HSP 1 Score: 56.225 bits (134), Expect = 1.493e-12
Identity = 25/48 (52.08%), Postives = 33/48 (68.75%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            C LP   G C+A IE+F++N+   KC+ FVFGGC  N NNF ++K CE
Sbjct:   13 CKLPKFAGPCMASIERFYYNAKIGKCQSFVFGGCLPNGNNFKTRKACE 60

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: ENSORLT00000042019.1 (pep primary_assembly:ASM223467v1:12:15740119:15780496:1 gene:ENSORLG00000010354.2 transcript:ENSORLT00000042019.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.4622 bits (145), Expect = 2.253e-12
Identity = 21/52 (40.38%), Postives = 38/52 (73.08%), Query Frame = 3

Query:   96 SRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            ++ P+  +P + G+C  Y E++F+N    +CE+F++GGC GN+NNF ++K+C
Sbjct:   76 TKLPQPHIPPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKEC 127

HSP 2 Score: 55.4546 bits (132), Expect = 1.551e-10
Identity = 24/67 (35.82%), Postives = 40/67 (59.70%), Query Frame = 3

Query:   51 IIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            I+ T +L    S    +   C    + G+C  Y E++F+N    +CE+F++GGC GN+NNF ++K+C
Sbjct:    5 ILGTFILEVGSSGPYKKVRVCAQAPDSGLCFGYFERYFYNFRKGRCEIFIYGGCLGNQNNFLTEKEC 71

HSP 3 Score: 51.2174 bits (121), Expect = 5.526e-9
Identity = 19/47 (40.43%), Postives = 32/47 (68.09%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDC 251
            C L  + G C  Y E++F+N    +CE+F++G C GN+NNF ++++C
Sbjct:  133 CTLDPDPGPCECYSERYFYNFKKRRCEIFIYGCCNGNQNNFETEQEC 179

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: wfikkn2a (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:NCBI gene;Acc:101168544])

HSP 1 Score: 60.4622 bits (145), Expect = 5.134e-12
Identity = 31/80 (38.75%), Postives = 46/80 (57.50%), Query Frame = 3

Query:   18 FMFTLIKMNSIIIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            F ++    N     T   C +   GE  AP C LP+ +G C AY  ++ ++S   KC+ FV+GGC GN+NNF S++ CE+
Sbjct:  358 FTYSQCNKNQNHFDTYEACMLACGGELAAP-CSLPSLQGPCKAYEPRWAYSSTLKKCQSFVYGGCGGNENNFESKEACEQ 436

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: wfikkn1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:NCBI gene;Acc:406570])

HSP 1 Score: 59.6918 bits (143), Expect = 8.756e-12
Identity = 24/48 (50.00%), Postives = 35/48 (72.92%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            C LPA +G C A+  ++ +NS   +C+ F FGGCQGN NNF+++K+CE
Sbjct:  362 CSLPAVQGPCKAWEARWAWNSMLKECQPFAFGGCQGNANNFHTKKECE 409

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:NCBI gene;Acc:101174930])

HSP 1 Score: 58.9214 bits (141), Expect = 1.257e-11
Identity = 30/80 (37.50%), Postives = 45/80 (56.25%), Query Frame = 3

Query:   24 FTLIKMNSIIIATLVLCSVISFGESRAPE--CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
             T   M   ++    L S +  G S  P+  C L  ++G C   IE++F+N+ T KCE+F +GGC GN NNF S + C++
Sbjct:   53 LTRATMEFGMLTLFALFSSLPGGLSLVPKGACLLRVDEGPCRGQIERYFYNTITQKCEVFYYGGCHGNANNFMSFQKCQK 132

HSP 2 Score: 54.6842 bits (130), Expect = 4.297e-10
Identity = 22/49 (44.90%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  111 CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            C  P +KG C A + +F++N AT  CE F + GC G+ NNF S++ CE+
Sbjct:  204 CLDPLDKGKCSASMRRFYYNKATKTCEEFAYSGCGGSSNNFVSRESCED 252

HSP 3 Score: 51.2174 bits (121), Expect = 8.213e-9
Identity = 25/64 (39.06%), Postives = 33/64 (51.56%), Query Frame = 3

Query:   72 CSVISFGESRAPE-CFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            C    F   + P+ C  P E+G C A    +FFN +T +CE F +GGC GN N F     C +F
Sbjct:  130 CQKTCFRIPKVPQICRFPKEEGHCRALFPSYFFNMSTMQCEPFSYGGCGGNSNRFRDLTSCMDF 193

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Match: paplnb (papilin [Source:NCBI gene;Acc:101161692])

HSP 1 Score: 58.9214 bits (141), Expect = 1.593e-11
Identity = 22/52 (42.31%), Postives = 35/52 (67.31%), Query Frame = 3

Query:  102 APECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            A  C LP ++G C  ++ +F +++A  KC+ F +GGC GN NNF S+ DC++
Sbjct:  749 ASACSLPRDEGPCEKWVARFAYDAAVGKCKEFWYGGCHGNANNFASEHDCQK 800

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000037994.1 (SMESG000037994.1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.095e-25
Identity = 40/72 (55.56%), Postives = 52/72 (72.22%), Query Frame = 3

Query:   39 MNSIIIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCE 254
            MNSI+I TL+ C + S GESRAPEC  P + GMC AY  +F+FN  +  CE F++GGCQ N NNFN+ ++CE
Sbjct:    1 MNSIVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECE 72

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000037995.1 (SMESG000037995.1)

HSP 1 Score: 78.9518 bits (193), Expect = 6.392e-21
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 3

Query:   39 MNSIIIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            M S I+  +VL S+    ESR  EC  P + GMC+AY ++F+FNS T KCE F++GGC  N NNF ++ +CEE 
Sbjct:    3 MKSKILVLVVLFSLSQLLESRLKECNEPKQSGMCMAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEEL 76

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000037999.1 (SMESG000037999.1)

HSP 1 Score: 77.0258 bits (188), Expect = 3.681e-20
Identity = 36/74 (48.65%), Postives = 48/74 (64.86%), Query Frame = 3

Query:   39 MNSIIIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            M S I+  +VL S+    ESR  EC  P + GMC AY ++F+FNS T KCE F++GGC  N NNF ++ +CEE 
Sbjct:    3 MKSKILVLVVLFSLSQLLESRLKECNEPKQSGMCEAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEEL 76

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000037999.1 (SMESG000037999.1)

HSP 1 Score: 76.6406 bits (187), Expect = 4.253e-20
Identity = 36/74 (48.65%), Postives = 48/74 (64.86%), Query Frame = 3

Query:   39 MNSIIIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEEF 260
            M S I+  +VL S+    ESR  EC  P + GMC AY ++F+FNS T KCE F++GGC  N NNF ++ +CEE 
Sbjct:    1 MKSKILVLVVLFSLSQLLESRLKECNEPKQSGMCEAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEEL 74

[Close]

BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Match: SMESG000038052.1 (SMESG000038052.1)

HSP 1 Score: 75.485 bits (184), Expect = 1.216e-19
Identity = 32/73 (43.84%), Postives = 49/73 (67.12%), Query Frame = 3

Query:   39 MNSIIIATLVLCSVISFGESRAPECFLPAEKGMCLAYIEKFFFNSATNKCELFVFGGCQGNKNNFNSQKDCEE 257
            MNS++I + +  +V+   ES++PEC LP E G+C AY  ++ F+    KC  FV+GGC  N NNF+++ +CEE
Sbjct:    1 MNSLVIFSCIFVAVLQLTESKSPECNLPLETGLCEAYFVRYGFDVKLGKCRTFVYGGCHVNDNNFHTRAECEE 73

The following BLAST results are available for this feature:

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Yeast
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
WFIKKN2	4.465e-13	53.06	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
WFIKKN2	4.525e-13	53.06	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
SPINT3	5.750e-13	52.00	serine peptidase inhibitor, Kunitz type 3 [Source:...	[more]
EPPIN	8.438e-12	42.19	epididymal peptidase inhibitor [Source:HGNC Symbol...	[more]
EPPIN	1.326e-11	42.19	epididymal peptidase inhibitor [Source:HGNC Symbol...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mlt-11	1.002e-14	49.06	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	1.012e-14	49.06	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	1.104e-14	49.06	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]
mlt-11	1.848e-14	49.06	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]
mlt-11	2.058e-14	49.06	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CG13748	1.238e-13	40.00	gene:FBgn0033355 transcript:FBtr0088656	[more]
CG3604	1.310e-12	48.00	gene:FBgn0031562 transcript:FBtr0077545	[more]
CG3604	1.310e-12	48.00	gene:FBgn0031562 transcript:FBtr0346581	[more]
CG10031	6.363e-11	39.22	gene:FBgn0031563 transcript:FBtr0077544	[more]
CG2816	3.269e-10	38.46	gene:FBgn0031564 transcript:FBtr0300299	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
wfikkn1	2.205e-12	45.83	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
wfikkn2a	3.813e-12	45.00	info WAP, follistatin/kazal, immunoglobulin, kunit...	[more]
wfikkn1	7.352e-12	45.83	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
wfikkn2b	2.529e-11	42.31	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
tfpia	5.712e-11	48.00	tissue factor pathway inhibitor a [Source:ZFIN;Acc...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tfpi2	4.329e-11	33.78	tissue factor pathway inhibitor 2 [Source:NCBI gen...	[more]
itch	1.489e-10	39.29	itchy E3 ubiquitin protein ligase [Source:Xenbase;...	[more]
cmtr2	1.966e-10	44.00	cap methyltransferase 2 [Source:Xenbase;Acc:XB-GEN...	[more]
cmtr2	2.393e-10	44.00	cap methyltransferase 2 [Source:Xenbase;Acc:XB-GEN...	[more]
itch	3.065e-10	41.51	itchy E3 ubiquitin protein ligase [Source:Xenbase;...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Spint3	2.841e-13	46.30	serine peptidase inhibitor, Kunitz type, 3 [Source...	[more]
Wfikkn2	3.559e-13	53.06	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
Eppin	4.479e-13	47.92	epididymal peptidase inhibitor [Source:MGI Symbol;...	[more]
Wfdc6a	1.626e-12	47.06	WAP four-disulfide core domain 6A [Source:MGI Symb...	[more]
Wfdc6a	1.626e-12	47.06	WAP four-disulfide core domain 6A [Source:MGI Symb...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P00992\|VKT3_VIPAA	3.156e-16	51.02	Kunitz-type serine protease inhibitor 3 OS=Vipera ...	[more]
sp\|P0DJ50\|VKT31_MESMA	1.469e-15	38.03	Kunitz-type serine protease inhibitor BmKTT-2 OS=M...	[more]
sp\|E5AJX3\|VKT_VIPBN	2.191e-15	51.02	Kunitz-type serine protease inhibitor ki-VN OS=Vip...	[more]
sp\|A8Y7N7\|VKTC4_DABSI	4.471e-15	44.64	Kunitz-type serine protease inhibitor C4 OS=Daboia...	[more]
sp\|P00991\|VKT1_VIPAA	4.471e-15	44.64	Kunitz-type serine protease inhibitor 1 OS=Vipera ...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A016W7U9	1.610e-14	46.67	BPTI/Kunitz inhibitor domain-containing protein (F...	[more]
A0A1S5QJJ2	3.007e-14	53.06	Kunitz/BPTI inhibitor-1 OS=Vipera ammodytes ammody...	[more]
A0A1S5QK73	4.653e-14	51.02	Kunitz/BPTI inhibitor-5 OS=Vipera ammodytes ammody...	[more]
A0A1S5QKN1	6.435e-14	51.02	Kunitz/BPTI inhibitor-7 OS=Vipera ammodytes ammody...	[more]
A0A1S5QJJ1	6.649e-14	51.02	Kunitz/BPTI inhibitor-3 OS=Vipera ammodytes ammody...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000039940.1	1.349e-13	46.94	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000051114.1	2.718e-13	39.39	pep primary_assembly:Astyanax_mexicanus-2.0:11:518...	[more]
tfpi2	1.046e-12	41.18	tissue factor pathway inhibitor 2 [Source:NCBI gen...	[more]
wfikkn2a	1.799e-11	43.33	WAP, Kazal, immunoglobulin, Kunitz and NTR domain-...	[more]
ambp	6.518e-11	42.00	alpha-1-microglobulin/bikunin precursor [Source:NC...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tfpi2	1.087e-11	42.59	tissue factor pathway inhibitor 2 [Source:ZFIN;Acc...	[more]
ENSPMAT00000005038.1	3.747e-11	46.81	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]
ENSPMAT00000005043.1	3.754e-11	46.81	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]
WFIKKN1	1.078e-10	40.82	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
WFIKKN1	3.629e-10	40.82	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO41696	3.358e-15	52.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO26263	3.358e-15	52.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO47795	1.434e-13	42.86	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41922	1.948e-13	48.98	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38284	1.493e-12	52.08	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000042019.1	2.253e-12	40.38	pep primary_assembly:ASM223467v1:12:15740119:15780...	[more]
wfikkn2a	5.134e-12	38.75	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
wfikkn1	8.756e-12	50.00	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
tfpi2	1.257e-11	37.50	tissue factor pathway inhibitor 2 [Source:NCBI gen...	[more]
paplnb	1.593e-11	42.31	papilin [Source:NCBI gene;Acc:101161692]	[more]

BLAST of Kunitz-type serine protease inhibitor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000037994.1	2.095e-25	55.56	SMESG000037994.1	[more]
SMESG000037995.1	6.392e-21	48.65	SMESG000037995.1	[more]
SMESG000037999.1	3.681e-20	48.65	SMESG000037999.1	[more]
SMESG000037999.1	4.253e-20	48.65	SMESG000037999.1	[more]
SMESG000038052.1	1.216e-19	43.84	SMESG000038052.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30017004 ID=SMED30017004|Name=Kunitz-type serine protease inhibitor|organism=Schmidtea mediterranea sexual|type=transcript|length=262bp
AAATAAATTTGAAATTCTTCATGTTCACATTAATAAAAATGAATTCAATA
ATTATTGCAACTTTAGTTTTATGTTCAGTTATAAGCTTTGGAGAAAGTCG
AGCTCCTGAATGCTTTTTACCGGCTGAGAAAGGAATGTGCCTTGCATACA
TTGAAAAATTCTTTTTCAATTCTGCTACTAATAAATGCGAACTATTCGTT
TTTGGTGGTTGCCAAGGCAATAAGAACAATTTTAATTCTCAAAAAGATTG
TGAAGAATTTTG

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000020	protonephridia
PLANA:0000026	gut
PLANA:0000067	goblet cell
PLANA:0000421	tail
PLANA:0002032	epidermal cell
PLANA:0002075	pigment cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036880	Kunitz_BPTI_sf
IPR002223	Kunitz_BPTI
IPR020901	Prtase_inh_Kunz-CS

Vocabulary: **molecular function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0030414	peptidase inhibitor activity

Vocabulary: **cellular component**
Term	Definition
GO:0005576	extracellular region

Vocabulary: **biological process**
Term	Definition
GO:0010466	negative regulation of peptidase activity
GO:0010951	negative regulation of endopeptidase activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PRINTS	PR00759	BASICPTASE	coord: 72..86 score: 40.78 coord: 34..48 score: 34.04 coord: 62..72 score: 55.19
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	SMART	SM00131	KU_2	coord: 35..86 e-value: 3.9E-15 score: 66.3
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PFAM	PF00014	Kunitz_BPTI	coord: 37..86 e-value: 8.6E-17 score: 61.1
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PROSITE	PS50279	BPTI_KUNITZ_2	coord: 37..86 score: 13.913
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	CDD	cd00109	KU	coord: 37..86 e-value: 6.03338E-19 score: 70.7418
IPR036880	Pancreatic trypsin inhibitor Kunitz domain superfamily	GENE3D	G3DSA:4.10.410.10		coord: 20..86 e-value: 2.9E-19 score: 70.7
IPR036880	Pancreatic trypsin inhibitor Kunitz domain superfamily	SUPERFAMILY	SSF57362	BPTI-like	coord: 32..85
None	No IPR available	PANTHER	PTHR10083	KUNITZ-TYPE PROTEASE INHIBITOR-RELATED	coord: 34..85
None	No IPR available	TMHMM	TMhelix		coord: 5..27
IPR020901	Proteinase inhibitor I2, Kunitz, conserved site	PROSITE	PS00280	BPTI_KUNITZ_1	coord: 65..83

Main menu

Kunitz-type serine protease inhibitor

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Owlery

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