Anoctamin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016964 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9Note: Hover over icons to view figure legend
Homology
BLAST of Anoctamin vs. Ensembl Human
Match: ANO4 (anoctamin 4 [Source:HGNC Symbol;Acc:HGNC:23837]) HSP 1 Score: 411.764 bits (1057), Expect = 2.854e-126 Identity = 317/991 (31.99%), Postives = 496/991 (50.05%), Query Frame = 1
Query: 91 TIMSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRK-----TIFQRLFET----FDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERP-XXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIY------IIGYSENG--FLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
T S++ L A + + K +NG++F DG +ID++LVY + N KR F +++ EGL++E KE+S ++D I+F+K+ A WE L RYAE + +R +++ L Y F + + F+R D + ++ E +T P+S+ + + F I + TFF A RS I V IL R E+ NK G+ +L++ G +++A+ LHE +++ + N R+LLY+ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P + +G+ V LY + + V KE+ Q T+I MCP+ + C F +LSD+ + L DNGATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y +VA+ LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT P R + + C GCL+DL +Q+ IM+ KQ ++ E P + + R K++Q S + E D + ++P + Y LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y G E G + G+ + SLS S+F + G + + GT YCRY +R PP PY YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 50 TSTSDDASRLEAGGETVPERNK-SNGLYFRDGKCRIDYILVYRKSNPQTE---KREVFERNIRAEGLQME-----------KESSLINSD----------IIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEEND----CYTAPFSQQRIHHFII-HNKETFFNNATRSRI--------------------------VHHILQR--------------------------------IKYEEGKNK---IGLNRLLTNGSYEAAFPLHEGSYRSKNSIRTHGAENHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHSQVSKEVCQATDIIMCPV--CDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERMNPISGKPEPYQAF-TDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGPERKISFPQWEKD--------------------YNLQP--MNAYG-----------LFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFS-GTPLKYCRYRDYRDPP--HSLVPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKER 908
BLAST of Anoctamin vs. Ensembl Human
Match: ANO4 (anoctamin 4 [Source:HGNC Symbol;Acc:HGNC:23837]) HSP 1 Score: 411.379 bits (1056), Expect = 8.587e-126 Identity = 317/992 (31.96%), Postives = 496/992 (50.00%), Query Frame = 1
Query: 88 FTIMSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRK-----TIFQRLFET----FDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERP-XXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIY------IIGYSENG--FLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
T S++ L A + + K +NG++F DG +ID++LVY + N KR F +++ EGL++E KE+S ++D I+F+K+ A WE L RYAE + +R +++ L Y F + + F+R D + ++ E +T P+S+ + + F I + TFF A RS I V IL R E+ NK G+ +L++ G +++A+ LHE +++ + N R+LLY+ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P + +G+ V LY + + V KE+ Q T+I MCP+ + C F +LSD+ + L DNGATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y +VA+ LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT P R + + C GCL+DL +Q+ IM+ KQ ++ E P + + R K++Q S + E D + ++P + Y LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y G E G + G+ + SLS S+F + G + + GT YCRY +R PP PY YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 84 LTSTSDDASRLEAGGETVPERNK-SNGLYFRDGKCRIDYILVYRKSNPQTE---KREVFERNIRAEGLQME-----------KESSLINSD----------IIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEEND----CYTAPFSQQRIHHF-IIHNKETFFNNATRSRI--------------------------VHHILQR--------------------------------IKYEEGKNK---IGLNRLLTNGSYEAAFPLHEGSYRSKNSIRTHGAENHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHSQVSKEVCQATDIIMCPV--CDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERMNPISGKPEPYQAF-TDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGPERKISFPQWEKD--------------------YNLQP--MNAYG-----------LFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFS-GTPLKYCRYRDYRDPP--HSLVPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKER 943
BLAST of Anoctamin vs. Ensembl Human
Match: ANO1 (anoctamin 1 [Source:HGNC Symbol;Acc:HGNC:21625]) HSP 1 Score: 405.986 bits (1042), Expect = 5.464e-125 Identity = 299/956 (31.28%), Postives = 474/956 (49.58%), Query Frame = 1
Query: 241 NQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR---KQMLSLGFEYDFNRK--TIFQRLFETFDFLNIFNINELKP-IPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPF-WRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQII-SHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPW-ETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMK-----------------NQHTSKLVADSFKKSA 3012
+ +R + +LL+ GLE+E +D+ K++ + F+K+ A W LCR AE LK++ K+M + +K ++ Q++ + + E +P + + P+S+ K++LFD+ D +FF + RS I V +IL R T C + GI L++ GV+ +AY LH+ D + ENVE N+R LLY+ W ++ QP+D ++ YFGEK+ YFAWLG YT L+P S++GIIV LY +I E+ Q NITMCPL + C +WK+S A + L DN ATV +V M++WA+ F+E WKR+Q++L Y W++ E E++ R E+ A + + KK + KE + WR R P +++ IA+ A + G+I+Y++ + + M+ S ++ + + AT +++LV+I+ L VY +A WLT +E +T+ ++ + K +LL+F+N+Y+ IFY+ F + P FRS M C GGCL++L +QL+ IM+GKQ+I +++ E +P++ ++ KLKQ + + R EV + +EP +A L EY+EM+IQFGF+T+F+ +FPLAPL AL NN+ EIR DA KF LRRPVA R KDIGIW +I+ + +AV NA +I+F + +I +L+Y+ YS+NG ++GF + +LS N S+F L LG CRY +R PPW E KY+ + +W +LAA++ FV +F+NLV + VIPD+P + + +E L E+ ++ + + + N H +K DS A
Sbjct: 8 KRFRREEYEGNLLEAGLELE-RDEDTKIH--------------------GVGFVKIHAPWNVLCREAEFLKLKMPTKKMYHINETRGLLKKINSVLQKITDPIQP----KVAEHRPQTMKRLSYPFSREKQHLFDL-SDKDSFFDSKTRSTI--------------------------VYEILKR---------------------TTC--------------TKAKYSMGITSLLANGVYAAAYPLHD----GDYNGENVEFNDRKLLYEEWARYGVFYKYQPIDLVRKYFGEKIGLYFAWLGVYTQMLIPASIVGIIVFLYGCATMDENIPSMEMCDQRHNITMCPL--CDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLKKES--RNKETDKVKLTWRDRFPAYLTNLVSIIFMIAVTFAIVLGVIIYRISMAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLIFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRM---EECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQQSPP--DHEECVKRKQRYEV---------------DYNLEP-----FAG----------LTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAVRAKDIGIWYNILRGIGKLAVIINAFVISFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSENKYD---ISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQDKQQLLETWMEKERQKDEPPCNHHNTKACPDSLGSPA 830
BLAST of Anoctamin vs. Ensembl Human
Match: ANO4 (anoctamin 4 [Source:HGNC Symbol;Acc:HGNC:23837]) HSP 1 Score: 412.92 bits (1060), Expect = 9.030e-125 Identity = 317/992 (31.96%), Postives = 496/992 (50.00%), Query Frame = 1
Query: 88 FTIMSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRK-----TIFQRLFET----FDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERP-XXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIY------IIGYSENG--FLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
T S++ L A + + K +NG++F DG +ID++LVY + N KR F +++ EGL++E KE+S ++D I+F+K+ A WE L RYAE + +R +++ L Y F + + F+R D + ++ E +T P+S+ + + F I + TFF A RS I V IL R E+ NK G+ +L++ G +++A+ LHE +++ + N R+LLY+ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P + +G+ V LY + + V KE+ Q T+I MCP+ + C F +LSD+ + L DNGATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y +VA+ LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT P R + + C GCL+DL +Q+ IM+ KQ ++ E P + + R K++Q S + E D + ++P + Y LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y G E G + G+ + SLS S+F + G + + GT YCRY +R PP PY YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 250 LTSTSDDASRLEAGGETVPERNK-SNGLYFRDGKCRIDYILVYRKSNPQTE---KREVFERNIRAEGLQME-----------KESSLINSD----------IIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEEND----CYTAPFSQQRIHHFII-HNKETFFNNATRSRI--------------------------VHHILQR--------------------------------IKYEEGKNK---IGLNRLLTNGSYEAAFPLHEGSYRSKNSIRTHGAENHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHSQVSKEVCQATDIIMCPV--CDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERMNPISGKPEPYQAF-TDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGPERKISFPQWEKD--------------------YNLQP--MNAYG-----------LFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFS-GTPLKYCRYRDYRDPP--HSLVPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKER 1109
BLAST of Anoctamin vs. Ensembl Human
Match: ANO1 (anoctamin 1 [Source:HGNC Symbol;Acc:HGNC:21625]) HSP 1 Score: 402.905 bits (1034), Expect = 2.549e-122 Identity = 319/1081 (29.51%), Postives = 512/1081 (47.36%), Query Frame = 1
Query: 97 MSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVY--ERINDNRT---------------------------------------------NQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR---KQMLSLGFEYDFNRK--TIFQRLFETFDFLNIFNINELKP-IPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA----RPEFVA-LIDKNYPKKTNLISGKEEPFIP--------------------------FWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQII-SHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPW-ETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMK-----------------NQHTSKLVADSFKKSA 3012
+ +E +L + + D + + KY G++F DG RK+D++LVY +R + NRT + +R + +LL+ GLE+E +D+ K++ + F+K+ A W LCR AE LK++ K+M + +K ++ Q++ + + E +P + + P+S+ K++LFD+ D +FF + RS I V +IL R T C + GI L++ GV+ +AY LH+ D + ENVE N+R LLY+ W ++ QP+D ++ YFGEK+ YFAWLG YT L+P S++GIIV LY +I E+ Q NITMCPL + C +WK+S A + L DN ATV +V M++WA+ F+E WKR+Q++L Y W++ E E+E R E+ A +++K+ K++ + ++ IP WR R P +++ IA+ A + G+I+Y++ + + M+ S ++ + + AT +++LV+I+ L VY +A WLT +E +T+ ++ + K +LL+F+N+Y+ IFY+ F + P FRS M C GGCL++L +QL+ IM+GKQ+I +++ E +P++ ++ KLKQ + + R EV + +EP +A L EY+EM+IQFGF+T+F+ +FPLAPL AL NN+ EIR DA KF LRRPVA R KDIGIW +I+ + +AV NA +I+F + +I +L+Y+ YS+NG ++GF + +LS N S+F L LG CRY +R PPW E KY+ + +W +LAA++ FV +F+NLV + VIPD+P + + +E L E+ ++ + + + N H +K DS A
Sbjct: 31 LPSEGTLLNSLSVDPDAECKY--GLYFRDGRRKVDYILVYHHKRPSGNRTLVRRVQHSDTPSGARSVKQDHPLPGKGASLDAGSGEPPMDYHEDDKRFRREEYEGNLLEAGLELE-RDEDTKIH--------------------GVGFVKIHAPWNVLCREAEFLKLKMPTKKMYHINETRGLLKKINSVLQKITDPIQP----KVAEHRPQTMKRLSYPFSREKQHLFDL-SDKDSFFDSKTRSTI--------------------------VYEILKR---------------------TTC--------------TKAKYSMGITSLLANGVYAAAYPLHD----GDYNGENVEFNDRKLLYEEWARYGVFYKYQPIDLVRKYFGEKIGLYFAWLGVYTQMLIPASIVGIIVFLYGCATMDENIPSMEMCDQRHNITMCPL--CDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLKKESR--NKEKRRHIPEESTNKWKQRVKTAMAGVKLTDKVKLTWRDRFPAYLTNLVSIIFMIAVTFAIVLGVIIYRISMAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLIFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRM---EECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQQSPP--DHEECVKRKQRYEV---------------DYNLEP-----FAG----------LTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAVRAKDIGIWYNILRGIGKLAVIINAFVISFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSENKYD---ISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQDKQQLLETWMEKERQKDEPPCNHHNTKACPDSLGSPA 976
BLAST of Anoctamin vs. Ensembl Celegans
Match: anoh-1 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:C0P286]) HSP 1 Score: 155.221 bits (391), Expect = 2.648e-38 Identity = 175/694 (25.22%), Postives = 301/694 (43.37%), Query Frame = 1
Query: 580 RGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILH----ERPXXXXXXXXXXXXXXXXXXYKHWVSMRNI-------FRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDI--------VVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFF------LIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFT---QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLI 2577
R P+ + LF D +FFT+++R + IL + ID+ +D+ R TD PS + E + K G++ L+ V++ A++LH E P + +V+ + R++ ++ QPL+ I+ YFGE++A+YFAW G + L P + G++V +Y + + + VV I Q N+ C R + + FF + D AV MSIW S+F+++WKR L+Y WN D + EP RPEF + K + I+G++ P R + ++ S V +S +M + + +++ L + + ++ + + A S+L+ + M L +YS + L E+HRT+S++++S+ +K++ + +N Y+S+FY+ FI+P + QP F + K C C L+LQL + K K+ LP VK ++L+ + EA RVE+ D+A +L + W ++P D +L E E +I FG +F FPLAPLLAL ++R DA + R+P+ IGIW+ I++ + AV +NA ++AF + + +
Sbjct: 111 RFINAPFQRKHGSLFLNYHDEKSFFTSSQRGYLTYQILTK----------------IDISKDLKGERLGESQDEPTD---------------PSTSSITSDEQLRRK----GLSWLLMSDVYEEAFVLHAPSKEEPYFKAMQNGSVKAYNEFISEIELDPRRSLSLNWERWYKFQPLNKIRDYFGEQIAYYFAWQGTFLTLLWPAVIFGLVVFIYGFIDSISSAPLDWNHCKVVNFIGQTENVA-----CGMR--------NGVTLFFSMVTQWFMSSFDTKMNAFFAVFMSIWGSVFVQIWKRNNSVLSYQWNSDDF-HAIEPDRPEF-----RGSKVKEDPITGEDIWISPALARYIKML---ASFVFVSFSMLVVVISLMLVTLLKIWMVYNFQCTKEYTFHCWLSAAFLPSILNTLSAMGLGAIYSNLVSRLNSWENHRTESEHNNSLIVKIFAFQMVNTYTSLFYVAFIRPESHGLQPNGLFGLGTEFKDT-CLDDTCSSLLALQLLTHTLIKPFPKFFKDVVLPYF---VKLFRLRMYTSR--------TEA-RVEIEDDDQANVL----VREW-LKPSA----GDFVL---------WEMNEKIILFGTTMMFASLFPLAPLLALIIGFVDMRIDAHRLIWFNRKPIPIITNGIGIWLPILTFLQYCAVFTNAFIVAFTSGFCSTFL 716
BLAST of Anoctamin vs. Ensembl Celegans
Match: anoh-1 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:C0P286]) HSP 1 Score: 155.221 bits (391), Expect = 2.750e-38 Identity = 175/694 (25.22%), Postives = 301/694 (43.37%), Query Frame = 1
Query: 580 RGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILH----ERPXXXXXXXXXXXXXXXXXXYKHWVSMRNI-------FRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDI--------VVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFF------LIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFT---QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLI 2577
R P+ + LF D +FFT+++R + IL + ID+ +D+ R TD PS + E + K G++ L+ V++ A++LH E P + +V+ + R++ ++ QPL+ I+ YFGE++A+YFAW G + L P + G++V +Y + + + VV I Q N+ C R + + FF + D AV MSIW S+F+++WKR L+Y WN D + EP RPEF + K + I+G++ P R + ++ S V +S +M + + +++ L + + ++ + + A S+L+ + M L +YS + L E+HRT+S++++S+ +K++ + +N Y+S+FY+ FI+P + QP F + K C C L+LQL + K K+ LP VK ++L+ + EA RVE+ D+A +L + W ++P D +L E E +I FG +F FPLAPLLAL ++R DA + R+P+ IGIW+ I++ + AV +NA ++AF + + +
Sbjct: 129 RFINAPFQRKHGSLFLNYHDEKSFFTSSQRGYLTYQILTK----------------IDISKDLKGERLGESQDEPTD---------------PSTSSITSDEQLRRK----GLSWLLMSDVYEEAFVLHAPSKEEPYFKAMQNGSVKAYNEFISEIELDPRRSLSLNWERWYKFQPLNKIRDYFGEQIAYYFAWQGTFLTLLWPAVIFGLVVFIYGFIDSISSAPLDWNHCKVVNFIGQTENVA-----CGMR--------NGVTLFFSMVTQWFMSSFDTKMNAFFAVFMSIWGSVFVQIWKRNNSVLSYQWNSDDF-HAIEPDRPEF-----RGSKVKEDPITGEDIWISPALARYIKML---ASFVFVSFSMLVVVISLMLVTLLKIWMVYNFQCTKEYTFHCWLSAAFLPSILNTLSAMGLGAIYSNLVSRLNSWENHRTESEHNNSLIVKIFAFQMVNTYTSLFYVAFIRPESHGLQPNGLFGLGTEFKD-TCLDDTCSSLLALQLLTHTLIKPFPKFFKDVVLPYF---VKLFRLRMYTSR--------TEA-RVEIEDDDQANVL----VREW-LKPSA----GDFVL---------WEMNEKIILFGTTMMFASLFPLAPLLALIIGFVDMRIDAHRLIWFNRKPIPIITNGIGIWLPILTFLQYCAVFTNAFIVAFTSGFCSTFL 734
BLAST of Anoctamin vs. Ensembl Celegans
Match: anoh-2 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:H2L2L3]) HSP 1 Score: 113.62 bits (283), Expect = 3.689e-25 Identity = 98/351 (27.92%), Postives = 156/351 (44.44%), Query Frame = 1
Query: 1069 QPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIV-SLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDM---EYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYS-TVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVK 2106
QP+D +K YFG ++A YFAWLG+ T L SLLGI++ L Y ++ +++YQ L+ + VL A IW++++LE WKR Q +LA+ W +D Y +P RP F D P N +SG+ EPF P W+ + TY T L + M A L + L F S M S + +L+I++S +Y R+A+ L D+E++RT +Y+ + K+ L +F+ + S+FYI F+ R + L LA ++I +Q+ +I E +P ++ +K
Sbjct: 269 QPIDQVKDYFGTEIAMYFAWLGHMTTALWFPSLLGILMWFLGGFKYKNSPGDKQDLYQ--------------------------------LISDICFVLFAFFNCIWSTIYLEWWKRVQAELAFKWGTYDATQDSYLQDP-RPAFQG--DYLAP---NPVSGRLEPFYPAWKHTVMRYVITYPITFLCVVGMFAAMLAIFTVQDLADFYFGD-------SWMLSWLCYFPMIFYALMIVIS-DKIYRRLALILNDLENYRTDDEYEDFLITKIVLFQFVTAFGSLFYIAFV---------LRD--------------MKRLQETLATLLITRQVTQNIMETVVPFMIEKLK 550 HSP 2 Score: 68.1662 bits (165), Expect = 3.478e-11 Identity = 48/109 (44.04%), Postives = 66/109 (60.55%), Query Frame = 1
Query: 2221 CIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIA 2547
C D+ PEL + ++++S RPL ++LEM IQFG++ +F PAFPLA + AL NNL EIR DA K ++RP +RVKDIG W M ++ + V N LI
Sbjct: 656 CTDS---NPELTQAELESVMSVYA-RPL-DDFLEMFIQFGYVLLFSPAFPLAAVCALINNLIEIRVDAFKLCNTVQRPFGRRVKDIGAWQKAMEILGILGVIVNCALIG 759
BLAST of Anoctamin vs. Ensembl Celegans
Match: anoh-2 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:H2L2L3]) HSP 1 Score: 112.849 bits (281), Expect = 4.734e-25 Identity = 98/351 (27.92%), Postives = 156/351 (44.44%), Query Frame = 1
Query: 1069 QPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIV-SLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDM---EYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYS-TVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVK 2106
QP+D +K YFG ++A YFAWLG+ T L SLLGI++ L Y ++ +++YQ L+ + VL A IW++++LE WKR Q +LA+ W +D Y +P RP F D P N +SG+ EPF P W+ + TY T L + M A L + L F S M S + +L+I++S +Y R+A+ L D+E++RT +Y+ + K+ L +F+ + S+FYI F+ R + L LA ++I +Q+ +I E +P ++ +K
Sbjct: 213 QPIDQVKDYFGTEIAMYFAWLGHMTTALWFPSLLGILMWFLGGFKYKNSPGDKQDLYQ--------------------------------LISDICFVLFAFFNCIWSTIYLEWWKRVQAELAFKWGTYDATQDSYLQDP-RPAFQG--DYLAP---NPVSGRLEPFYPAWKHTVMRYVITYPITFLCVVGMFAAMLAIFTVQDLADFYFGD-------SWMLSWLCYFPMIFYALMIVIS-DKIYRRLALILNDLENYRTDDEYEDFLITKIVLFQFVTAFGSLFYIAFV---------LRD--------------MKRLQETLATLLITRQVTQNIMETVVPFMIEKLK 494 HSP 2 Score: 68.1662 bits (165), Expect = 3.204e-11 Identity = 48/109 (44.04%), Postives = 66/109 (60.55%), Query Frame = 1
Query: 2221 CIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIA 2547
C D+ PEL + ++++S RPL ++LEM IQFG++ +F PAFPLA + AL NNL EIR DA K ++RP +RVKDIG W M ++ + V N LI
Sbjct: 600 CTDS---NPELTQAELESVMSVYA-RPL-DDFLEMFIQFGYVLLFSPAFPLAAVCALINNLIEIRVDAFKLCNTVQRPFGRRVKDIGAWQKAMEILGILGVIVNCALIG 703
BLAST of Anoctamin vs. Ensembl Fly
Match: subdued (gene:FBgn0038721 transcript:FBtr0083806) HSP 1 Score: 482.256 bits (1240), Expect = 4.584e-153 Identity = 334/943 (35.42%), Postives = 502/943 (53.23%), Query Frame = 1
Query: 160 NNGMFFVDGVRKIDFVLVYERINDNRTNQI----KRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNR--KTIFQRLFETFDFL--NIFNINELKPI-PRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQG--TNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQ-PFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYG-GHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKAD 2949
N +FF D VR IDFVL Y RIN + ++ KR F +L+ +GLE+E S + I F+K+ A E L RYAE+LK+R M + NR K++F L F F NI+ E+ P FT YS+ KEYLFDI +D FFT A RS I VE IL R+ + P+ ++ D++ FGI +LI+EGV+ +AY LH+ R LLYKHW S+ +R QPLD IK YFG K+ YFAWLGYYTY LL S++G+I LY+ N + VK+I Q TNITMCPL C C FW L + + + L+DN +TV AV MS WA+LFLE+WKR ++ + W++ + +E RP+++A ++ P + + ++ +EP +PFWR ++P F++S VLL IA+A L +++Y++ + +++ S ++ + ++ A+ + ++L ++ L +Y+ +A +LT++E RTQ+Q+D S+TLK+YLL+F+N Y+SIFYI F + F + + C +GGCL +L +QLA IM+GKQ + I E LP V ++ +L N + +A KD+ + D K +D W G R LF EYLEM++Q+GF+TIF+ AFPLAP AL NN+ E+R DA K +RPV+QRV+DIG+W I+ + ++V +N +IAF + I +L+Y ++ G L+G+ +F+LS + + TLY L +N T CRY+ FR PP + E Y+ + +++ ILA ++ FV +FEN V L M ++ IPD+ +LR ++RE +TNEII+ +
Sbjct: 33 NTSLFFEDCVRSIDFVLAY-RINAHEPTELENTEKRRVFEANLISQGLEVE------------------------SSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKEIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQD--CFFTTAVRSRI--------------------------VEFILDRQ-----------------------RFPAKNQ----HDMA-----FGIERLIAEGVYSAAYPLHD-------GEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPL-CD--WCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKV--LAIQVGLSRLFNNTPNPDKA-------KDERWMRDFKLLD-W------------------GARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVID-SPTLYSLAGDL---SNITTCRYTDFRLPP--SSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQE 846
BLAST of Anoctamin vs. Ensembl Fly
Match: subdued (gene:FBgn0038721 transcript:FBtr0334921) HSP 1 Score: 482.256 bits (1240), Expect = 4.584e-153 Identity = 334/943 (35.42%), Postives = 502/943 (53.23%), Query Frame = 1
Query: 160 NNGMFFVDGVRKIDFVLVYERINDNRTNQI----KRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNR--KTIFQRLFETFDFL--NIFNINELKPI-PRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQG--TNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQ-PFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYG-GHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKAD 2949
N +FF D VR IDFVL Y RIN + ++ KR F +L+ +GLE+E S + I F+K+ A E L RYAE+LK+R M + NR K++F L F F NI+ E+ P FT YS+ KEYLFDI +D FFT A RS I VE IL R+ + P+ ++ D++ FGI +LI+EGV+ +AY LH+ R LLYKHW S+ +R QPLD IK YFG K+ YFAWLGYYTY LL S++G+I LY+ N + VK+I Q TNITMCPL C C FW L + + + L+DN +TV AV MS WA+LFLE+WKR ++ + W++ + +E RP+++A ++ P + + ++ +EP +PFWR ++P F++S VLL IA+A L +++Y++ + +++ S ++ + ++ A+ + ++L ++ L +Y+ +A +LT++E RTQ+Q+D S+TLK+YLL+F+N Y+SIFYI F + F + + C +GGCL +L +QLA IM+GKQ + I E LP V ++ +L N + +A KD+ + D K +D W G R LF EYLEM++Q+GF+TIF+ AFPLAP AL NN+ E+R DA K +RPV+QRV+DIG+W I+ + ++V +N +IAF + I +L+Y ++ G L+G+ +F+LS + + TLY L +N T CRY+ FR PP + E Y+ + +++ ILA ++ FV +FEN V L M ++ IPD+ +LR ++RE +TNEII+ +
Sbjct: 33 NTSLFFEDCVRSIDFVLAY-RINAHEPTELENTEKRRVFEANLISQGLEVE------------------------SSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKEIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQD--CFFTTAVRSRI--------------------------VEFILDRQ-----------------------RFPAKNQ----HDMA-----FGIERLIAEGVYSAAYPLHD-------GEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPL-CD--WCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKV--LAIQVGLSRLFNNTPNPDKA-------KDERWMRDFKLLD-W------------------GARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVID-SPTLYSLAGDL---SNITTCRYTDFRLPP--SSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQE 846
BLAST of Anoctamin vs. Ensembl Fly
Match: subdued (gene:FBgn0038721 transcript:FBtr0083805) HSP 1 Score: 482.256 bits (1240), Expect = 2.732e-151 Identity = 334/943 (35.42%), Postives = 502/943 (53.23%), Query Frame = 1
Query: 160 NNGMFFVDGVRKIDFVLVYERINDNRTNQI----KRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNR--KTIFQRLFETFDFL--NIFNINELKPI-PRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQG--TNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQ-PFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYG-GHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKAD 2949
N +FF D VR IDFVL Y RIN + ++ KR F +L+ +GLE+E S + I F+K+ A E L RYAE+LK+R M + NR K++F L F F NI+ E+ P FT YS+ KEYLFDI +D FFT A RS I VE IL R+ + P+ ++ D++ FGI +LI+EGV+ +AY LH+ R LLYKHW S+ +R QPLD IK YFG K+ YFAWLGYYTY LL S++G+I LY+ N + VK+I Q TNITMCPL C C FW L + + + L+DN +TV AV MS WA+LFLE+WKR ++ + W++ + +E RP+++A ++ P + + ++ +EP +PFWR ++P F++S VLL IA+A L +++Y++ + +++ S ++ + ++ A+ + ++L ++ L +Y+ +A +LT++E RTQ+Q+D S+TLK+YLL+F+N Y+SIFYI F + F + + C +GGCL +L +QLA IM+GKQ + I E LP V ++ +L N + +A KD+ + D K +D W G R LF EYLEM++Q+GF+TIF+ AFPLAP AL NN+ E+R DA K +RPV+QRV+DIG+W I+ + ++V +N +IAF + I +L+Y ++ G L+G+ +F+LS + + TLY L +N T CRY+ FR PP + E Y+ + +++ ILA ++ FV +FEN V L M ++ IPD+ +LR ++RE +TNEII+ +
Sbjct: 182 NTSLFFEDCVRSIDFVLAY-RINAHEPTELENTEKRRVFEANLISQGLEVE------------------------SSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKEIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQD--CFFTTAVRSRI--------------------------VEFILDRQ-----------------------RFPAKNQ----HDMA-----FGIERLIAEGVYSAAYPLHD-------GEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPL-CD--WCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKV--LAIQVGLSRLFNNTPNPDKA-------KDERWMRDFKLLD-W------------------GARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVID-SPTLYSLAGDL---SNITTCRYTDFRLPP--SSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQE 995
BLAST of Anoctamin vs. Ensembl Fly
Match: subdued (gene:FBgn0038721 transcript:FBtr0334922) HSP 1 Score: 472.241 bits (1214), Expect = 6.377e-149 Identity = 335/967 (34.64%), Postives = 503/967 (52.02%), Query Frame = 1
Query: 160 NNGMFFVDGVRKIDFVLVYERINDNRTNQI----KRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQM---------------LSLGFEYDFNR-----------KTIFQRLFETFDFL--NIFNINELKPI-PRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQG--TNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQ-PFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYG-GHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKAD 2949
N +FF D VR IDFVL Y RIN + ++ KR F +L+ +GLE+E S + I F+K+ A E L RYAE+LK+R M L Y N+ K++F L F F NI+ E+ P FT YS+ KEYLFDI +D FFT A RS I VE IL R+ + P+ ++ D++ FGI +LI+EGV+ +AY LH+ R LLYKHW S+ +R QPLD IK YFG K+ YFAWLGYYTY LL S++G+I LY+ N + VK+I Q TNITMCPL C C FW L + + + L+DN +TV AV MS WA+LFLE+WKR ++ + W++ + +E RP+++A ++ P + + ++ +EP +PFWR ++P F++S VLL IA+A L +++Y++ + +++ S ++ + ++ A+ + ++L ++ L +Y+ +A +LT++E RTQ+Q+D S+TLK+YLL+F+N Y+SIFYI F + F + + C +GGCL +L +QLA IM+GKQ + I E LP V ++ +L N + +A KD+ + D K +D W G R LF EYLEM++Q+GF+TIF+ AFPLAP AL NN+ E+R DA K +RPV+QRV+DIG+W I+ + ++V +N +IAF + I +L+Y ++ G L+G+ +F+LS + + TLY L +N T CRY+ FR PP + E Y+ + +++ ILA ++ FV +FEN V L M ++ IPD+ +LR ++RE +TNEII+ +
Sbjct: 33 NTSLFFEDCVRSIDFVLAY-RINAHEPTELENTEKRRVFEANLISQGLEVE------------------------SSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKESLCNLRISERSNRLRNAAHYLTNKIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQD--CFFTTAVRSRI--------------------------VEFILDRQ-----------------------RFPAKNQ----HDMA-----FGIERLIAEGVYSAAYPLHD-------GEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPL-CD--WCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKV--LAIQVGLSRLFNNTPNPDKA-------KDERWMRDFKLLD-W------------------GARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVID-SPTLYSLAGDL---SNITTCRYTDFRLPP--SSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQE 870
BLAST of Anoctamin vs. Ensembl Fly
Match: subdued (gene:FBgn0038721 transcript:FBtr0302492) HSP 1 Score: 472.241 bits (1214), Expect = 2.753e-147 Identity = 335/967 (34.64%), Postives = 503/967 (52.02%), Query Frame = 1
Query: 160 NNGMFFVDGVRKIDFVLVYERINDNRTNQI----KRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQM---------------LSLGFEYDFNR-----------KTIFQRLFETFDFL--NIFNINELKPI-PRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQG--TNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQ-PFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYG-GHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKAD 2949
N +FF D VR IDFVL Y RIN + ++ KR F +L+ +GLE+E S + I F+K+ A E L RYAE+LK+R M L Y N+ K++F L F F NI+ E+ P FT YS+ KEYLFDI +D FFT A RS I VE IL R+ + P+ ++ D++ FGI +LI+EGV+ +AY LH+ R LLYKHW S+ +R QPLD IK YFG K+ YFAWLGYYTY LL S++G+I LY+ N + VK+I Q TNITMCPL C C FW L + + + L+DN +TV AV MS WA+LFLE+WKR ++ + W++ + +E RP+++A ++ P + + ++ +EP +PFWR ++P F++S VLL IA+A L +++Y++ + +++ S ++ + ++ A+ + ++L ++ L +Y+ +A +LT++E RTQ+Q+D S+TLK+YLL+F+N Y+SIFYI F + F + + C +GGCL +L +QLA IM+GKQ + I E LP V ++ +L N + +A KD+ + D K +D W G R LF EYLEM++Q+GF+TIF+ AFPLAP AL NN+ E+R DA K +RPV+QRV+DIG+W I+ + ++V +N +IAF + I +L+Y ++ G L+G+ +F+LS + + TLY L +N T CRY+ FR PP + E Y+ + +++ ILA ++ FV +FEN V L M ++ IPD+ +LR ++RE +TNEII+ +
Sbjct: 182 NTSLFFEDCVRSIDFVLAY-RINAHEPTELENTEKRRVFEANLISQGLEVE------------------------SSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKESLCNLRISERSNRLRNAAHYLTNKIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQD--CFFTTAVRSRI--------------------------VEFILDRQ-----------------------RFPAKNQ----HDMA-----FGIERLIAEGVYSAAYPLHD-------GEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPL-CD--WCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKV--LAIQVGLSRLFNNTPNPDKA-------KDERWMRDFKLLD-W------------------GARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVID-SPTLYSLAGDL---SNITTCRYTDFRLPP--SSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQE 1019
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: ano3 (anoctamin 3 [Source:ZFIN;Acc:ZDB-GENE-111031-3]) HSP 1 Score: 415.231 bits (1066), Expect = 5.138e-127 Identity = 310/951 (32.60%), Postives = 470/951 (49.42%), Query Frame = 1
Query: 163 NGMFFVDGVRKIDFVLVYERINDNRTNQI-KRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDN---ATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXX-XXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPR--GCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYII--------GYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
+G+ F DG ++ID++LVY++ + Q+ KR TF ++L EGL +E KE S ++D I+F+K+ A W+TLC+YAE + IR + D+ +K + R F L + +PR P KE L D+ E + A F A +N R ++ S + I V +L R ED +K GI +L+ +++A+ HE + N R+LLY+ W ++ QPLD I+ YFGEK+ YFAWLG+YT L+P +L+G+ V LY + + V KEI + MCP MC C W LSD+ + L DNG TV A+ M+IWA++FLE WKR++ +L Y W++ D E E+E RP+F A + ++ N ISGK EPF P ++ + + S + I++ L + +++++L++ + T+ TG ++ +IIMSL +VY +VA LT++EH RT+S++++S LK++L +F+N SS FYI F P + + R C GCL+DL LQ+ IM+ KQI ++ E P L + R K+K+A E QS+++ E P+ W ++P ++ G L EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+ R DIGIW ++ + +AV +NA +IA + YI + +Y I GY L G+ + SLS + E Y YCRY +R PPW EPY +T +W +LAA++ F+ +FE+LV+ IA +IPD+P L M+RE L E++ +A+L ++ +
Sbjct: 153 SGLMFRDGKKRIDYILVYKK----SSPQVEKRCTFERNLRAEGLMLE-----------KEPSLTNSD----------IMFVKIHAPWDTLCKYAEQMNIRMPFRKKCYFTDWKKKAMGSRFQLRFRQLKSW-------LPR---NPMKLDKEALPDLVETDCYTAPFCRARMHHFTIN---NRETFFSNSTRSRI-------VHHVLQRT--------------------------------KYEDGKSK---MGINRLLGNSTYEAAFPPHEGCYKSRHPIKTHGAQNHRHLLYERWARWGIWYKYQPLDLIRRYFGEKIGLYFAWLGWYTGMLIPAALVGLFVFLYGLFTMDSSQVSKEICEANTTVMCP-MCEG-NCSSWTLSDSCVYAKVTHLFDNGGTVFFAIFMAIWATVFLEFWKRRRAELTYDWDLIDWEEEEEELRPQFEAKYSRK--ERVNPISGKPEPFQPL-TDKLSRLMVSVSGIFFMISLVLTAVFAVVVFRLIAMEKFVSFQWEFVKKNWQFATSGTGVCINFMIIMSLNVVYEKVAYLLTNLEHPRTESEWENSFALKMFLFQFVNLNSSTFYIAFFLGRFAGRPGDYNKLFNRWRMEECHPSGCLIDLCLQMGVIMVLKQIWNNFMELGYPLLQNWWSRRKMKRAGE----QSNSKEEL---------------PQWDRDWNLQP----------MNAHG---LVDEYLEMVLQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPMPARATDIGIWHGVLEGIGVVAVITNAFVIAITSDYIPRFVYAIKYGPCVDKGYRHEKCLRGYLNNSLSVFDMGELRNGSY---------ENQYCRYRDYRAPPWSP--EPYEFTLQFWHVLAARLAFIIVFEHLVFGIKTFIAHMIPDMPKDLCDRMRREKYLMQEMVYEAELEHLQKE 975
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: ano5a (anoctamin 5a [Source:ZFIN;Acc:ZDB-GENE-091013-2]) HSP 1 Score: 399.438 bits (1025), Expect = 6.887e-122 Identity = 306/992 (30.85%), Postives = 482/992 (48.59%), Query Frame = 1
Query: 100 SNED-EILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFT-TPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDID--VVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDE--PARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSII-----TAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLI--YIIGYSENGFLNGFTDFSLSYMNSSEF----------NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
SN D E +N +++ ++ + ++F DGVR+IDFVL Y D Q +R F +L K GLE+E +D S S F+K+ WE L YA+VLKI+ + F+ + +I E K IP G+ TP+ + P+ FFT+ +++ L I DK + P +V +LSR P++ ED K GI +L++ G + SA+ LH+ ++ +ER LYK+W + ++ QPL+ I+ Y+GEK+ YFAWLG+YT L +++G++ +Y + +I +EI G I MCPL C + C +WKLS S + L DN ATV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ + K ++ N ++ + EP++P + + +TVL I++ +A + G+I Y+L F S+M+ + + + ++I T+ T S ++ VIIM L +Y RVA+ +TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P F S ++ C GGCL++L+ QL +M+GKQ+ +I+E +P L + + E L + R D+ L + G LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ E+R DA KF RRPVA + IG W I++M+A +V +NA +++F + I +L+ Y+ ++G+ SLS N SEF N + N T CRY +R+PP K Y++ +W ILAAK+ F+ + E++V++ +A +IPDVP ++ +KRE L E + ++ ++KNQ
Sbjct: 32 SNSDKETFQLENAEVDKQQQSKDSVYFRDGVRRIDFVLSYIDDKDGEKKQDRRKVFEANLQKAGLEVETEDK-------------------SESEDGKTYFLKIHVPWEVLATYADVLKIK-----VPFKAN--------------------DIPENKEIPMGWLFTPFRLPDHVMNPEPD----FFTSTFDKSKIDFFL-------IEDKETLFPASTRNRIVYYLLSR------------------------------IPYSTEDKDKK----GIKRLLNNGTYTSAFPLHD-CRYWTRSRDDNCESERYSLYKNWARFSSFYKEQPLNLIRKYYGEKIGIYFAWLGFYTEMLSYAAVVGLLCFIYGVATFDENIWSREICNETIGGQIVMCPL-CDKK-CGYWKLSTTCSSSWQSYLFDNAATVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYES---KCTNRRLNRVTQEMEPYLPL-TSKCARSILSGATVLFWISLIIASIIGVIAYRLAVFAAFASIMKDSPTNKLEIVGNLITPQFATSVTASCINFVIIMVLNFLYERVAIKITDMEVPKTHVEYENKLTIKMFLFQFVNYYSSCFYVAFFKGKFVGYPGNYAYMFGPWSGLRNEECEPGGCLIELTTQLVIVMVGKQVWGNIQEALVPWLRNWWVSRSARNHPESLYS-------------------------------------RWEQDHDLQNLGQLGLFYEYLEMVIQFGFITLFVASFPLAPLLALMNNILEVRVDAWKFTTQFRRPVAAKAHSIGAWEEILNMIAVFSVVTNAFIVSFTSDMIPRLVYQYVHNTGNAASMSGYITHSLSIYNISEFPPENRPDDDENPDWFN------AGNYTTCRYRDYRYPPGHEK--QYTHNMDFWHILAAKLAFIIIMEHVVFVVKFFVAWLIPDVPSDVKARVKRERYLIQEYLHDYEVEKLKNQ 882
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: ano5b (anoctamin 5b [Source:ZFIN;Acc:ZDB-GENE-061215-108]) HSP 1 Score: 396.741 bits (1018), Expect = 6.340e-121 Identity = 308/997 (30.89%), Postives = 481/997 (48.24%), Query Frame = 1
Query: 136 DINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSEN--GFLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNE-TYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ---------HTSKLVADSFKKSAVIAK 3024
+I+ + + +FF DGVR+IDFVL Y + +R F +L K GLE+E +D S S ++K+ A WE L YA+VLKI+ R+ + L F N ++P P FT P+ KSK F I +D TFF + R+ IV YIL RC P+ ED K GI +L++ G + SAY LH+ + +ER LY++W ++ QPL+ IK Y+GEK+ YFAWLG+YT L +++G+I +Y ++ ++I KEI G I MCPL + C +WKL+ + L DN TV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K ++ N I+ + EP++PF + + +TVL + +A + G+I Y+L + S+M+ + I + S+IT + T S ++ VII+ L +Y VA+W+TDME +T +Y++ +T+K+++ +F+N YSS FY+ F + P F S ++ C GGCL++L+ QL +M GKQ++ +++E LP + + K +S E R E D+ L + LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ E+R DA KF RRP+A + ++IG W I+++VA ++V +NA ++AF + I +L+Y+ Y + G+ SLS N S+ N G + ++ T CRY +R+PP + Y++T +W ILAAK+ F+ + E++V++ +A +IPDVP +++ +KRE L E + ++ ++K Q T+ L+ S K VI++
Sbjct: 46 EIDKFDQNRDSVFFKDGVRRIDFVLSYVDDKEGDKKAERRREFEANLEKAGLELETEDK-------------------SESDDRKTHYLKIHAPWEVLATYADVLKIKVPFKVSDIPK--AREVPLEWLSHPFRLPE----NIMRPEPDYFTAPFDKSKVDFFLI-DDKDTFFPPSTRNRIVYYILT---------------------------RC-----------------------------PYYKEDRKEKDKT-GINRLLNNGTYTSAYPLHD-CRYWKKAQDMQCESERYHLYRYWARFLCFYKEQPLNLIKKYYGEKIGIYFAWLGFYTEMLFYAAVMGVICFVYGVLSYEDNITSKEICDPKIGGMIVMCPL--CDKKCSYWKLNSTCLSSWQSHLFDNEGTVFFAMFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQIRPEYE---QKCTGRRLNRITQEMEPYLPF-PSKCARFCLSGATVLFWTCLIVACIMGVIAYRLAVYAAFASVMKDSSTSKIQLVGSLITPQLATSVTASCINFVIILILNFLYEHVAIWITDMEIPKTHLEYENKLTMKMFMFQFVNYYSSCFYVAFFKGKFVGYPGNYSYMFGKWSTLRNEECAPGGCLIELTTQLLIVMAGKQMVGNVQEALLPLVRNWWSSRK---------GRSHPESTYSRWE----------------------------QDHDLQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNILEVRVDAWKFTTQFRRPMAAKARNIGAWEEILNVVAIMSVVTNAFIMAFTSDMIPRLVYLYAYHPGIEANMTGYITNSLSIYNISQIPEDNLPEAGENPSWFNSSTITTCRYRDYRYPPGHLR--QYTHTMQFWHILAAKLAFIIIMEHVVFVVKFFVAWLIPDVPSEVKARIKRERFLVQEYLHNYEVEKLKMQLSQSFCLSTETASLLPSSPNKHQVISE 913
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: ano5a (anoctamin 5a [Source:ZFIN;Acc:ZDB-GENE-091013-2]) HSP 1 Score: 392.119 bits (1006), Expect = 1.114e-119 Identity = 301/978 (30.78%), Postives = 475/978 (48.57%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFT-TPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDID--VVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDE--PARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSII-----TAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLI--YIIGYSENGFLNGFTDFSLSYMNSSEF----------NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
++ ++ + ++F DGVR+IDFVL Y D Q +R F +L K GLE+E +D S S F+K+ WE L YA+VLKI+ + F+ + +I E K IP G+ TP+ + P+ FFT+ +++ L I DK + P +V +LSR P++ ED K GI +L++ G + SA+ LH+ ++ +ER LYK+W + ++ QPL+ I+ Y+GEK+ YFAWLG+YT L +++G++ +Y + +I +EI G I MCPL C + C +WKLS S + L DN ATV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ + K ++ N ++ + EP++P + + +TVL I++ +A + G+I Y+L F S+M+ + + + ++I T+ T S ++ VIIM L +Y RVA+ +TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P F S ++ C GGCL++L+ QL +M+GKQ+ +I+E +P L + + E L + R D+ L + G LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ E+R DA KF RRPVA + IG W I++M+A +V +NA +++F + I +L+ Y+ ++G+ SLS N SEF N + N T CRY +R+PP K Y++ +W ILAAK+ F+ + E++V++ +A +IPDVP ++ +KRE L E + ++ ++KNQ
Sbjct: 23 VDKQQQSKDSVYFRDGVRRIDFVLSYIDDKDGEKKQDRRKVFEANLQKAGLEVETEDK-------------------SESEDGKTYFLKIHVPWEVLATYADVLKIK-----VPFKAN--------------------DIPENKEIPMGWLFTPFRLPDHVMNPEPD----FFTSTFDKSKIDFFL-------IEDKETLFPASTRNRIVYYLLSR------------------------------IPYSTEDKDKK----GIKRLLNNGTYTSAFPLHD-CRYWTRSRDDNCESERYSLYKNWARFSSFYKEQPLNLIRKYYGEKIGIYFAWLGFYTEMLSYAAVVGLLCFIYGVATFDENIWSREICNETIGGQIVMCPL-CDKK-CGYWKLSTTCSSSWQSYLFDNAATVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYES---KCTNRRLNRVTQEMEPYLPL-TSKCARSILSGATVLFWISLIIASIIGVIAYRLAVFAAFASIMKDSPTNKLEIVGNLITPQFATSVTASCINFVIIMVLNFLYERVAIKITDMEVPKTHVEYENKLTIKMFLFQFVNYYSSCFYVAFFKGKFVGYPGNYAYMFGPWSGLRNEECEPGGCLIELTTQLVIVMVGKQVWGNIQEALVPWLRNWWVSRSARNHPESLYS-------------------------------------RWEQDHDLQNLGQLGLFYEYLEMVIQFGFITLFVASFPLAPLLALMNNILEVRVDAWKFTTQFRRPVAAKAHSIGAWEEILNMIAVFSVVTNAFIVSFTSDMIPRLVYQYVHNTGNAASMSGYITHSLSIYNISEFPPENRPDDDENPDWFN------AGNYTTCRYRDYRYPPGHEK--QYTHNMDFWHILAAKLAFIIIMEHVVFVVKFFVAWLIPDVPSDVKARVKRERYLIQEYLHDYEVEKLKNQ 859
BLAST of Anoctamin vs. Ensembl Zebrafish
Match: ano11 (anoctamin 11 [Source:ZFIN;Acc:ZDB-GENE-090313-245]) HSP 1 Score: 376.711 bits (966), Expect = 1.999e-116 Identity = 241/698 (34.53%), Postives = 385/698 (55.16%), Query Frame = 1
Query: 898 KTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXX-XXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNI-TMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSM-IKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIIL--KADLLQMKNQHT 2976
K + G+A+L+ +G F A+ LHE P + N+R +LY +W S + QPLDHI+ YFGEK+A YFAWLG+YT WLLP +L+G V + I+ ++ KEI + + MCPL C + C+ W +SD + L D+ TV +V MS+WA FLE WKR+ LA+HW+ D ++EP RPEF A+ + N ++G +EP+ P + R+ M +++ + + + FL +I+Y+ + +M S + + I + +L++L +I+ + VY+ +A LT E HRTQ+QY+ + T K+++ +F+N YSS FY+ F + P + ++ C GGCL++L+ QL IM+GKQ+I++I+E+ +P+ +K ++ K+A + ++ E +R W+ + ELI C LF EYLE+++QFGFITIF+ AFPLAPL AL NN E+R DA KF RRPVA+R + IG+W I+ ++ ++V NA LIAF + ++ +L+Y Y N L+G+ +F+L+Y S +N ++ T CRY FR E Y VYW++LA ++ F+ FE++V+ + +I ++PD+P+ L++ +KRE L + + + LLQ K T
Sbjct: 14 KRAEVGVARLLRDGAFTGAFPLHEGPFKLPSCDTQPDQLNKRQVLYHYWASWLKWCKYQPLDHIREYFGEKIALYFAWLGFYTAWLLPAALVGTCVFVSGILTMGSNTPAKEICESGGLYLMCPL-CET--CKPWNMSDICPMAKVGYLFDHPGTVFFSVFMSLWAVTFLEYWKRKNATLAHHWDCMDFHEDEEPPRPEFAAMAPA---MEENPVTGVKEPYFPE-KARISRMLTGSMVIVIMLCVVMIFLVTVIIYRSIVSVMMFETGSSVLRTQAGNIANISSTLVNLALILLMGQVYTALAEQLTKWEMHRTQTQYEDAFTFKVFIFQFVNFYSSPFYVAFFKGRFVGYPGHYGTLFGMRNEDCGPGGCLIELAEQLCIIMVGKQLINNIQEFVIPK----IKAWRQKRALAS-VKKAQKTQEPER-------------------WEQDYELIPCEG-----------LFDEYLEIVLQFGFITIFVAAFPLAPLFALLNNWVEVRLDAHKFVCEYRRPVAERAQHIGVWFIILEALSHVSVVVNAFLIAFTSDFLPRLLY--QYKFNNDLHGYVNFTLAYSPPS-YNH-----------SSHTVCRYKAFRD-------ENGKYKLVYWELLAVRLGFIIAFEHVVFFVLRVIDWMVPDIPESLQLKIKRERYLAKQALADNQEALLQAKRLAT 648
BLAST of Anoctamin vs. Ensembl Xenopus
Match: ANO5 (anoctamin 5 [Source:NCBI gene;Acc:100489787]) HSP 1 Score: 400.593 bits (1028), Expect = 1.153e-122 Identity = 314/974 (32.24%), Postives = 473/974 (48.56%), Query Frame = 1
Query: 115 ILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVY--ERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHS-RTISNM--SSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRG--CPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYS--ENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
+L P I+ + + +FF DG R+IDFVL Y + D +R F +L K GLE+E +D S F+K+ A WE L YAEVL ++ + + + D + I + + F + P P FT P+ K K+ LF E+ FFT + R+ IV Y ILSR C + E+ K FGI +L++ G + AY LH+ + + N ER LY HW ++ QPLD I+ Y+GEK+ YFAWLG+YT L +++G LY + + I KEI G I MCPL C R C FW+L+ + DN AT+ A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ + K KK N ++ + EP++P + F+ +TVL IA+ +A + II+Y+L + S+M + + + +SN+ + T+ T S+L+ V IM L +Y RVA+W+TDME RT +Y++ +T+K++L +F+N YSS FY+ F + P+ S K R C GCL++L+ QL +M GKQI +I+E +P + +KR K + + L + R D L G LF EYLEM++QFGFIT+F+ +FPLAPLLAL NN+ EIR D+ K +RPVA + IG+W I++ +A ++V +NA ++AF + I +L+Y YS E ++G+ SLS N S+F + N CRY +R+PP K Y T YW ILAAK+ F+ + E++V+L +A +IPDVP +++ +KRE LT +I+ + +L ++K + +K
Sbjct: 15 LLPPPPPQIDKHAQSKDSVFFWDGSRRIDFVLSYTEDTGKDAEKKAERRREFELNLQKSGLELETEDK-------------------KESENGKTYFVKIHAPWEVLTTYAEVLNLKMPLKADDLKDD--SENILSHVLKLFKLPQ----EVMYPEPDYFTAPFRKDKQELFRT-EEREKFFTPSTRNRIVYY--------------------------ILSR-----------------------CH-------YGEEEGKKK---FGIKRLLTNGSYLDAYPLHD---CQYWKKTDERNCERYTLYTHWAKFTRFYKEQPLDLIRKYYGEKIGIYFAWLGFYTEMLFYAAVVGFFCFLYGWITMDDSISSKEICDPNIGGQIIMCPL-CDKR-CEFWRLNSTCEPSQYSHMFDNVATLFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYES---KCTEKKKNPVTQEMEPYLPA-TSKAARFCFSGATVLFWIALIIASIIAIIVYRLWVYAAFASIMDNNLALKPVSNLLTPQLATSVTASVLNFVTIMILNFLYERVAIWITDMEIPRTHLEYENRLTMKMFLFQFVNYYSSCFYVAFFKGKFVGYPADYSYLFGKWRNEECDPAGCLIELTTQLTIVMAGKQIWGNIQEAFVPWTWNWLKRRKARNHPQNLYS-------------------------------------RWEHDGDLQTFGGLGLFNEYLEMVVQFGFITLFVASFPLAPLLALLNNILEIRVDSWKLTTQFKRPVAAKAHSIGVWQEILNGIAILSVVTNAFIVAFTSDMIPRLVYYYAYSQDEGTPMSGYIGSSLSVFNVSDFKEQ---SMPMENDMNVISCRYRDYRYPPGHEK--EYQVTMQYWHILAAKMAFIIIMEHVVFLVKFFVAWLIPDVPSEVKARVKREKFLTQKILHEYELEKLKQKLCNK 852
BLAST of Anoctamin vs. Ensembl Xenopus
Match: ANO5 (anoctamin 5 [Source:NCBI gene;Acc:100489787]) HSP 1 Score: 400.208 bits (1027), Expect = 1.748e-122 Identity = 314/974 (32.24%), Postives = 473/974 (48.56%), Query Frame = 1
Query: 115 ILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVY--ERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHS-RTISNM--SSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRG--CPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYS--ENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
+L P I+ + + +FF DG R+IDFVL Y + D +R F +L K GLE+E +D S F+K+ A WE L YAEVL ++ + + + D + I + + F + P P FT P+ K K+ LF E+ FFT + R+ IV Y ILSR C + E+ K FGI +L++ G + AY LH+ + + N ER LY HW ++ QPLD I+ Y+GEK+ YFAWLG+YT L +++G LY + + I KEI G I MCPL C R C FW+L+ + DN AT+ A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ + K KK N ++ + EP++P + F+ +TVL IA+ +A + II+Y+L + S+M + + + +SN+ + T+ T S+L+ V IM L +Y RVA+W+TDME RT +Y++ +T+K++L +F+N YSS FY+ F + P+ S K R C GCL++L+ QL +M GKQI +I+E +P + +KR K + + L + R D L G LF EYLEM++QFGFIT+F+ +FPLAPLLAL NN+ EIR D+ K +RPVA + IG+W I++ +A ++V +NA ++AF + I +L+Y YS E ++G+ SLS N S+F + N CRY +R+PP K Y T YW ILAAK+ F+ + E++V+L +A +IPDVP +++ +KRE LT +I+ + +L ++K + +K
Sbjct: 15 LLPPPPPQIDKHAQSKDSVFFWDGSRRIDFVLSYTEDTGKDAEKKAERRREFELNLQKSGLELETEDK-------------------KESENGKTYFVKIHAPWEVLTTYAEVLNLKMPLKADDLKDD--SENILSHVLKLFKLPQ----EVMYPEPDYFTAPFRKDKQELFRT-EEREKFFTPSTRNRIVYY--------------------------ILSR-----------------------CH-------YGEEEGKKK---FGIKRLLTNGSYLDAYPLHD---CQYWKKTDERNCERYTLYTHWAKFTRFYKEQPLDLIRKYYGEKIGIYFAWLGFYTEMLFYAAVVGFFCFLYGWITMDDSISSKEICDPNIGGQIIMCPL-CDKR-CEFWRLNSTCEPSQYSHMFDNVATLFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYES---KCTEKKKNPVTQEMEPYLPA-TSKAARFCFSGATVLFWIALIIASIIAIIVYRLWVYAAFASIMDNNLALKPVSNLLTPQLATSVTASVLNFVTIMILNFLYERVAIWITDMEIPRTHLEYENRLTMKMFLFQFVNYYSSCFYVAFFKGKFVGYPADYSYLFGKWRNEECDPAGCLIELTTQLTIVMAGKQIWGNIQEAFVPWTWNWLKRRKARNHPQNLYS-------------------------------------RWEHDGDLQTFGGLGLFNEYLEMVVQFGFITLFVASFPLAPLLALLNNILEIRVDSWKLTTQFKRPVAAKAHSIGVWQEILNGIAILSVVTNAFIVAFTSDMIPRLVYYYAYSQDEGTPMSGYIGSSLSVFNVSDFKEQ---SMPMENDMNVISCRYRDYRYPPGHEK--EYQVTMQYWHILAAKMAFIIIMEHVVFLVKFFVAWLIPDVPSEVKARVKREKFLTQKILHEYELEKLKQKLCNK 852
BLAST of Anoctamin vs. Ensembl Xenopus
Match: ANO4 (major histocompatibility complex class I-related gene protein-like [Source:Xenbase;Acc:XB-GENE-5756215]) HSP 1 Score: 399.053 bits (1024), Expect = 2.050e-122 Identity = 306/957 (31.97%), Postives = 481/957 (50.26%), Query Frame = 1
Query: 136 DINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXX-XXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSE-NGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
DI + G+FF DG R+ID++LVY + + KR F +++ EGL +E KE+S ++D I+F+K+ A WE L RYAE++ +R +++ L Y F L + L+ I +L+ +T P+S+ + + F I + TFF A R+ +V IL R E+ NK G+ +L++ +++A+ LHE + ++ R+LL++ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P +++G+ V LY + + V +EI Q TNITMCPL + C + +LSD+ + L DN ATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y RVA++LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT +P R + + C GCL+DL LQ+ IM+ KQ ++ E P + + R KL+Q E I K + P+ + ++P P LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y Y G + Y+N+S L++ + +S HPP + EPY+YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 3 DIGEIFPVCCGLFFNDGKRRIDYILVYRKTS---LQSEKREIFERNIRAEGLHLE-----------KESSIVNSD----------IIFVKLHAPWEVLGRYAELMNVRMPFRRKIYYLHRRYKFMSSRFRGLLPKKPMRLDKEKIPDLEE-NDCYTAPFSQQRIHHFII-NNKDTFFNNATRT--------------------------RIVHHILQR--------------------------------VKYEEGKNKI---GLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPL--CDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERVNPISGKPEPYQAF-ADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQ------------------EHGIHGKTAL--PQWEKDYNLQP-------------INPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCAGQGRAGEKCMVGYVNAS-----------LSVFLVTDFENHSNSSHPP--SAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKER 821
BLAST of Anoctamin vs. Ensembl Xenopus
Match: ANO4 (major histocompatibility complex class I-related gene protein-like [Source:Xenbase;Acc:XB-GENE-5756215]) HSP 1 Score: 397.127 bits (1019), Expect = 9.422e-122 Identity = 305/950 (32.11%), Postives = 473/950 (49.79%), Query Frame = 1
Query: 148 DKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGF--TTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXX-XXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
+K +G+FF DG R+ID++LVY + + KR F +++ EGL +E KE+S ++D I+F+K+ A WE L RYAE++ +R F RK + L + F++ F RG P KE + D+ E+N + + I ++I+ D +V IL R E+ NK G+ +L++ +++A+ LHE + ++ R+LL++ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P +++G+ V LY + + V +EI Q TNITMCPL + C + +LSD+ + L DN ATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L L Y RVA++LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT +P R + + C GCL+DL LQ+ IM+ KQ ++ E P + + R KL+Q E I K + P+ + ++P P LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y Y G KL + + RY +R PP + EPY+YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 18 EKNKTSGLFFNDGKRRIDYILVYRKTS---LQSEKREIFERNIRAEGLHLE-----------KESSIVNSD----------IIFVKLHAPWEVLGRYAELMNVRM---------PFRRKIYY--LHRRYKFMSRF---------RGLLPKKPMRLDKEKIPDL-EENDCYTAPFSQQRIHHFIINN------KDTFFNNATRTRIVHHILQR--------------------------------VKYEEGKNKI---GLNRLLTNCTYEAAFPLHEGSYRSKNSIKTHGAENHRHLLFECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAIVGLFVFLYGMFTLDSCQVSREICQATNITMCPL--CDKYCPYMRLSDSCIYAKVTHLFDNSATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERVNPISGKPEPYQAF-ADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNL-YERVALFLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGRPGAYLRLINKWRLEECHPSGCLIDLCLQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKLRQ------------------EHGIHGKTAL--PQWEKDYNLQP-------------INPYGLFEEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFITQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAVTSDFIPRLVYAYKYGPCA-------------------GQGRAGEKLIISFSS---RYRDYRDPP--SAPEPYAYTLQFWHVLAARLAFIIVFEHLVFFIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERVQKEKKER 818
BLAST of Anoctamin vs. Ensembl Xenopus
Match: ANO5 (anoctamin 5 [Source:NCBI gene;Acc:100489787]) HSP 1 Score: 386.341 bits (991), Expect = 2.181e-117 Identity = 309/974 (31.72%), Postives = 465/974 (47.74%), Query Frame = 1
Query: 115 ILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVY--ERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHS-RTISNM--SSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRG--CPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYS--ENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
+L P I+ + + +FF DG R+IDFVL Y + D +R F +L K GLE+E +D S F+K+ A WE L YAEVL ++ + + + D + I + + F + P P FT P+ K D F ER R +V ILSR C + E+ K FGI +L++ G + AY LH+ + + N ER LY HW ++ QPLD I+ Y+GEK+ YFAWLG+YT L +++G LY + + I KEI G I MCPL C R C FW+L+ + DN AT+ A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ + K KK N ++ + EP++P + F+ +TVL IA+ +A + II+Y+L + S+M + + + +SN+ + T+ T S+L+ V IM L +Y RVA+W+TDME RT +Y++ +T+K++L +F+N YSS FY+ F + P+ S K R C GCL++L+ QL +M GKQI +I+E +P + +KR K + + L + R D L G LF EYLEM++QFGFIT+F+ +FPLAPLLAL NN+ EIR D+ K +RPVA + IG+W I++ +A ++V +NA ++AF + I +L+Y YS E ++G+ SLS N S+F + N CRY +R+PP K Y T YW ILAAK+ F+ + E++V+L +A +IPDVP +++ +KRE LT +I+ + +L ++K + +K
Sbjct: 15 LLPPPPPQIDKHAQSKDSVFFWDGSRRIDFVLSYTEDTGKDAEKKAERRREFELNLQKSGLELETEDK-------------------KESENGKTYFVKIHAPWEVLTTYAEVLNLKMPLKADDLKDD--SENILSHVLKLFKLPQ----EVMYPEPDYFTAPFRK----------DKQELFRTEEREKFFTPSTRNR-----------------IVYYILSR-----------------------CH-------YGEEEGKKK---FGIKRLLTNGSYLDAYPLHD---CQYWKKTDERNCERYTLYTHWAKFTRFYKEQPLDLIRKYYGEKIGIYFAWLGFYTEMLFYAAVVGFFCFLYGWITMDDSISSKEICDPNIGGQIIMCPL-CDKR-CEFWRLNSTCEPSQYSHMFDNVATLFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYES---KCTEKKKNPVTQEMEPYLPA-TSKAARFCFSGATVLFWIALIIASIIAIIVYRLWVYAAFASIMDNNLALKPVSNLLTPQLATSVTASVLNFVTIMILNFLYERVAIWITDMEIPRTHLEYENRLTMKMFLFQFVNYYSSCFYVAFFKGKFVGYPADYSYLFGKWRNEECDPAGCLIELTTQLTIVMAGKQIWGNIQEAFVPWTWNWLKRRKARNHPQNLYS-------------------------------------RWEHDGDLQTFGGLGLFNEYLEMVVQFGFITLFVASFPLAPLLALLNNILEIRVDSWKLTTQFKRPVAAKAHSIGVWQEILNGIAILSVVTNAFIVAFTSDMIPRLVYYYAYSQDEGTPMSGYIGSSLSVFNVSDFKEQ---SMPMENDMNVISCRYRDYRYPPGHEK--EYQVTMQYWHILAAKMAFIIIMEHVVFLVKFFVAWLIPDVPSEVKARVKREKFLTQKILHEYELEKLKQKLCNK 852
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano1 (anoctamin 1, calcium activated chloride channel [Source:MGI Symbol;Acc:MGI:2142149]) HSP 1 Score: 417.542 bits (1072), Expect = 3.631e-128 Identity = 318/1006 (31.61%), Postives = 509/1006 (50.60%), Query Frame = 1
Query: 97 MSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEI--------QDDGC--KVYPFKENS------YYDNDS-VGSPSHRSNIV------------------FIKVFATWETLCRYAEVLKIR---KQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKP-IPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVA-LIDKNYPKKTNLISGKEEPFIPF-WRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQII-SHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
+ +E +L + + D + + KY G++F DG RK+D++LVY KR + ++L + GL+ ++ QD K P S Y+++D + N++ F+K+ A W LCR AE LK++ K++ + E KTI L + D + + E +P + + P+S+ K++LFD+ D +FF + RS I V +IL R T C + GI L++ GV+ +AY LH+ D + +NVE N+R LLY+ W S ++ QP+D ++ YFGEKV YFAWLG YT L+P S++G+IV LY +I E+ Q NITMCPL + C +WK+S A + L DN ATV +V M++WA+ F+E WKR+Q++L Y W++ E E++ R E+ A +++K+ K++ KE + WR R P F +++ IA+ A + G+I+Y++ + + + M+ S ++ + + AT +++LV+I+ L VY +A WLT +E +T+ ++ +T K +LL+F+N+Y+ IFY+ F + P FRS M C GGCL++L +QL+ IM+GKQ+I +++ E +P++ ++ KL++ + +DR E + + +D + +EP +A L EY+EM+IQFGF+T+F+ +FPLAPL AL NN+ EIR DA KF LRRPVA R KDIGIW +I+ V +AV NA +I+F + +I +L+Y+ YS+NG ++GF + +LS N S+F L LG CRY +R PPW Y + +W +LAA++ FV +F+NLV + VIPD+P + + +E L E+ ++ + Q K Q
Sbjct: 31 LPSEGTLLNSLSVDPDAECKY--GLYFRDGKRKVDYILVYHH---------KRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHIS-ETRGLLKTINSVLQKITDPIQP-KVAEHRPQTTKRLSYPFSREKQHLFDL-TDRDSFFDSKTRSTI--------------------------VYEILKR---------------------TTC--------------TKAKYSMGITSLLANGVYSAAYPLHD----GDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPL--CDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESR---NKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRM---EECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRR-----------QSPSDREEYVKRKQRYEVD------FNLEP-----FAG----------LTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSE--HKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREE--QGKQQ 913
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano1 (anoctamin 1, calcium activated chloride channel [Source:MGI Symbol;Acc:MGI:2142149]) HSP 1 Score: 418.313 bits (1074), Expect = 6.506e-128 Identity = 314/992 (31.65%), Postives = 502/992 (50.60%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEI--------QDDGC--KVYPFKENS------YYDNDS-VGSPSHRSNIV------------------FIKVFATWETLCRYAEVLKIR---KQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKP-IPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVA-LIDKNYPKKTNLISGKEEPFIPF-WRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQII-SHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
++ D + G++F DG RK+D++LVY KR + ++L + GL+ ++ QD K P S Y+++D + N++ F+K+ A W LCR AE LK++ K++ + E KTI L + D + + E +P + + P+S+ K++LFD+ D +FF + RS I V +IL R T C + GI L++ GV+ +AY LH+ D + +NVE N+R LLY+ W S ++ QP+D ++ YFGEKV YFAWLG YT L+P S++G+IV LY +I E+ Q NITMCPL + C +WK+S A + L DN ATV +V M++WA+ F+E WKR+Q++L Y W++ E E++ R E+ A +++K+ K++ KE + WR R P F +++ IA+ A + G+I+Y++ + + + M+ S ++ + + AT +++LV+I+ L VY +A WLT +E +T+ ++ +T K +LL+F+N+Y+ IFY+ F + P FRS M + C GGCL++L +QL+ IM+GKQ+I +++ E +P++ ++ KL++ + +DR E + + +D + +EP +A L EY+EM+IQFGF+T+F+ +FPLAPL AL NN+ EIR DA KF LRRPVA R KDIGIW +I+ V +AV NA +I+F + +I +L+Y+ YS+NG ++GF + +LS N S+F L LG CRY +R PPW Y + +W +LAA++ FV +F+NLV + VIPD+P + + +E L E+ ++ + Q K Q
Sbjct: 101 VDPDAECKYGLYFRDGKRKVDYILVYHH---------KRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHIS-ETRGLLKTINSVLQKITDPIQP-KVAEHRPQTTKRLSYPFSREKQHLFDL-TDRDSFFDSKTRSTI--------------------------VYEILKR---------------------TTC--------------TKAKYSMGITSLLANGVYSAAYPLHD----GDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPL--CDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESR---NKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEE---CAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRR-----------QSPSDREEYVKRKQRYEVD------FNLEP-----FAG----------LTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSE--HKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREE--QGKQQ 971
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano1 (anoctamin 1, calcium activated chloride channel [Source:MGI Symbol;Acc:MGI:2142149]) HSP 1 Score: 414.461 bits (1064), Expect = 1.649e-126 Identity = 315/996 (31.63%), Postives = 502/996 (50.40%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEI--------QDDGC--KVYPFKENS------YYDNDS-VGSPSHRSNIV------------------FIKVFATWETLCRYAEVLKIR---KQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKP-IPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA----RPEFVA-LIDKNYPKKTNLISGKEEPFIPF-WRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQII-SHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
++ D + G++F DG RK+D++LVY KR + ++L + GL+ ++ QD K P S Y+++D + N++ F+K+ A W LCR AE LK++ K++ + E KTI L + D + + E +P + + P+S+ K++LFD+ D +FF + RS I V +IL R T C + GI L++ GV+ +AY LH+ D + +NVE N+R LLY+ W S ++ QP+D ++ YFGEKV YFAWLG YT L+P S++G+IV LY +I E+ Q NITMCPL + C +WK+S A + L DN ATV +V M++WA+ F+E WKR+Q++L Y W++ E E+E R E+ A +++K+ K++ KE + WR R P F +++ IA+ A + G+I+Y++ + + + M+ S ++ + + AT +++LV+I+ L VY +A WLT +E +T+ ++ +T K +LL+F+N+Y+ IFY+ F + P FRS M + C GGCL++L +QL+ IM+GKQ+I +++ E +P++ ++ KL++ + +DR E + + +D + +EP +A L EY+EM+IQFGF+T+F+ +FPLAPL AL NN+ EIR DA KF LRRPVA R KDIGIW +I+ V +AV NA +I+F + +I +L+Y+ YS+NG ++GF + +LS N S+F L LG CRY +R PPW Y + +W +LAA++ FV +F+NLV + VIPD+P + + +E L E+ ++ + Q K Q
Sbjct: 100 VDPDAECKYGLYFRDGKRKVDYILVYHH---------KRASGSRTLARRGLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHIS-ETRGLLKTINSVLQKITDPIQP-KVAEHRPQTTKRLSYPFSREKQHLFDL-TDRDSFFDSKTRSTI--------------------------VYEILKR---------------------TTC--------------TKAKYSMGITSLLANGVYSAAYPLHD----GDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPL--CDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESR---NKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEE---CAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRR-----------QSPSDREEYVKRKQRYEVD------FNLEP-----FAG----------LTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSE--HKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREE--QGKQQ 974
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano4 (anoctamin 4 [Source:MGI Symbol;Acc:MGI:2443344]) HSP 1 Score: 411.379 bits (1056), Expect = 2.698e-126 Identity = 319/993 (32.12%), Postives = 496/993 (49.95%), Query Frame = 1
Query: 91 TIMSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRK-----TIFQRLFET----FDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERP-XXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQ--AEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIY------IIGYSENG--FLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
T S + L A + + K +NG++F DG +ID++LVY + N KR F +++ EGL++E KE+S ++D I+F+K+ A WE L RYAE + +R +++ L Y F + + F+R D + ++ E +T P+S+ + + F I + TFF A RS I V IL R E+ NK G+ +L++ G +++A+ LHE +++ + N R+LLY+ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P + +G+ V LY + + V KE+ Q T+I MCP+ + C F +LSD+ + L DNGATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y +VA+ LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT P R + + C GCL+DL +Q+ IM+ KQ ++ E P + + R K++Q E+ IN E + + L P + Y LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y G E G + G+ + SLS S+F + G + + GT YCRY +R PP PY YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 50 TSTSEDTSRLEAGGETVRERNK-SNGLYFRDGKCRIDYILVYRKSNPQTE---KREVFERNIRAEGLQME-----------KESSLINSD----------IIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEE----NDCYTAPFSQQRIHHF-IIHNKDTFFNNATRSRI--------------------------VHHILQR--------------------------------IKYEEGKNK---IGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTHGAVNHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHCQVSKEVCQATDIIMCPV--CDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERMNPISGKPEPYQAF-TDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGTERKINFPQWEKDYN------------LQP------------MNAYG-----------LFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFS-GTPLKYCRYRDYRDPP--HSLAPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKER 908
BLAST of Anoctamin vs. Ensembl Mouse
Match: Ano4 (anoctamin 4 [Source:MGI Symbol;Acc:MGI:2443344]) HSP 1 Score: 411.379 bits (1056), Expect = 2.698e-126 Identity = 319/993 (32.12%), Postives = 496/993 (49.95%), Query Frame = 1
Query: 91 TIMSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRK-----TIFQRLFET----FDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERP-XXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQ--AEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIY------IIGYSENG--FLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
T S + L A + + K +NG++F DG +ID++LVY + N KR F +++ EGL++E KE+S ++D I+F+K+ A WE L RYAE + +R +++ L Y F + + F+R D + ++ E +T P+S+ + + F I + TFF A RS I V IL R E+ NK G+ +L++ G +++A+ LHE +++ + N R+LLY+ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P + +G+ V LY + + V KE+ Q T+I MCP+ + C F +LSD+ + L DNGATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y +VA+ LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT P R + + C GCL+DL +Q+ IM+ KQ ++ E P + + R K++Q E+ IN E + + L P + Y LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y G E G + G+ + SLS S+F + G + + GT YCRY +R PP PY YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 50 TSTSEDTSRLEAGGETVRERNK-SNGLYFRDGKCRIDYILVYRKSNPQTE---KREVFERNIRAEGLQME-----------KESSLINSD----------IIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEE----NDCYTAPFSQQRIHHF-IIHNKDTFFNNATRSRI--------------------------VHHILQR--------------------------------IKYEEGKNK---IGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTHGAVNHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHCQVSKEVCQATDIIMCPV--CDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERMNPISGKPEPYQAF-TDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGTERKINFPQWEKDYN------------LQP------------MNAYG-----------LFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFS-GTPLKYCRYRDYRDPP--HSLAPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKER 908
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q8C5H1|ANO4_MOUSE (Anoctamin-4 OS=Mus musculus OX=10090 GN=Ano4 PE=1 SV=2) HSP 1 Score: 411.764 bits (1057), Expect = 3.720e-125 Identity = 318/994 (31.99%), Postives = 497/994 (50.00%), Query Frame = 1
Query: 88 FTIMSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRK-----TIFQRLFET----FDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERP-XXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQ--AEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIY------IIGYSENG--FLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
T S + L A + + K +NG++F DG +ID++LVY + N KR F +++ EGL++E KE+S ++D I+F+K+ A WE L RYAE + +R +++ L Y F + + F+R D + ++ E +T P+S+ + + F I + TFF A RS I V IL R E+ NK G+ +L++ G +++A+ LHE +++ + N R+LLY+ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P + +G+ V LY + + V KE+ Q T+I MCP+ + C F +LSD+ + L DNGATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y +VA+ LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT P R + + C GCL+DL +Q+ IM+ KQ ++ E P + + R K++Q E+ IN P+ + ++P + Y LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y G E G + G+ + SLS S+F + G + + GT YCRY +R PP PY YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 84 LTSTSEDTSRLEAGGETVRERNK-SNGLYFRDGKCRIDYILVYRKSNPQTE---KREVFERNIRAEGLQME-----------KESSLINSD----------IIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEE----NDCYTAPFSQQRIHHF-IIHNKDTFFNNATRSRI--------------------------VHHILQR--------------------------------IKYEEGKNK---IGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTHGAVNHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHCQVSKEVCQATDIIMCPV--CDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERMNPISGKPEPYQAF-TDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGTERKINF----------------------PQWEKDYNLQP--MNAYG-----------LFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFS-GTPLKYCRYRDYRDPP--HSLAPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKER 943
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q32M45|ANO4_HUMAN (Anoctamin-4 OS=Homo sapiens OX=9606 GN=ANO4 PE=2 SV=1) HSP 1 Score: 411.379 bits (1056), Expect = 4.124e-125 Identity = 317/992 (31.96%), Postives = 496/992 (50.00%), Query Frame = 1
Query: 88 FTIMSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRK-----TIFQRLFET----FDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERP-XXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIY------IIGYSENG--FLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
T S++ L A + + K +NG++F DG +ID++LVY + N KR F +++ EGL++E KE+S ++D I+F+K+ A WE L RYAE + +R +++ L Y F + + F+R D + ++ E +T P+S+ + + F I + TFF A RS I V IL R E+ NK G+ +L++ G +++A+ LHE +++ + N R+LLY+ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P + +G+ V LY + + V KE+ Q T+I MCP+ + C F +LSD+ + L DNGATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y +VA+ LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT P R + + C GCL+DL +Q+ IM+ KQ ++ E P + + R K++Q S + E D + ++P + Y LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y G E G + G+ + SLS S+F + G + + GT YCRY +R PP PY YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 84 LTSTSDDASRLEAGGETVPERNK-SNGLYFRDGKCRIDYILVYRKSNPQTE---KREVFERNIRAEGLQME-----------KESSLINSD----------IIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEEND----CYTAPFSQQRIHHF-IIHNKETFFNNATRSRI--------------------------VHHILQR--------------------------------IKYEEGKNK---IGLNRLLTNGSYEAAFPLHEGSYRSKNSIRTHGAENHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHSQVSKEVCQATDIIMCPV--CDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERMNPISGKPEPYQAF-TDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGPERKISFPQWEKD--------------------YNLQP--MNAYG-----------LFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFS-GTPLKYCRYRDYRDPP--HSLVPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKER 943
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|A6QLE6|ANO4_BOVIN (Anoctamin-4 OS=Bos taurus OX=9913 GN=ANO4 PE=2 SV=1) HSP 1 Score: 410.223 bits (1053), Expect = 4.584e-125 Identity = 317/991 (31.99%), Postives = 496/991 (50.05%), Query Frame = 1
Query: 91 TIMSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIR----KQMLSLGFEYDFNRK-----TIFQRLFET----FDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERP-XXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIY------IIGYSENG--FLNGFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
T S++ L A + + K N G++F DG +ID++LVY + N KR F +++ EGL++E KE+S ++D I+F+K+ A WE L RYAE + +R +++ L Y F + + F+R D + ++ E +T P+S+ + + F I + TFF A RS I V IL R E+ NK G+ +L++ G +++A+ LHE +++ + N R+LLY+ W S ++ QPLD ++ YFGEK+ YFAWLG+YT L P + +G+ V LY ++ + V KE+ Q T+I MCP+ + C F +LSD+ + L DNGATV AV M++WA++FLE WKR++ +AY W++ D E E+E RP+F A K ++ N ISGK EP+ F + + + S + I + +A + GI++Y++V+ S I N S + T T ++ IIM L ++Y +VA+ LT++E RT+S++++S TLK++L +F+N SS FYI F+ FT P R + + C GCL+DL +Q+ IM+ KQ ++ E P + + R K++Q S + E D + ++P + Y LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RRP+A R KDIGIW I+ + ++V +NA +IA + +I +L+Y G E G + G+ + SLS S+F + G + + GT YCRY +R PP PY YT +W +LAA++ F+ +FE+LV+ +I+ +IPD+P LR M+RE L E++ +A+L +++ + +
Sbjct: 50 TSTSDDASRLEAGGETVPEKNKLN-GLYFRDGKCRIDYILVYRKSNPQME---KREVFERNIRAEGLQME-----------KESSLINSD----------IIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEEND----CYTAPFSQQRIHHFII-HNKDTFFNNATRSRI--------------------------VHHILQR--------------------------------IKYEEGKNK---IGLNRLLTNGSYEAAFPLHEGSYRSKNSIRTHGAVNHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVITLDHCQVSKEVCQATDIIMCPV--CDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKK--ERMNPISGKPEPYQAF-ADKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGPERKISFPQWEKD--------------------YNLQP--MNAYG-----------LFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFS-GTPLKYCRYRDYRDPP--HSLVPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKER 908
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q8BHY3|ANO1_MOUSE (Anoctamin-1 OS=Mus musculus OX=10090 GN=Ano1 PE=1 SV=2) HSP 1 Score: 404.06 bits (1037), Expect = 2.504e-122 Identity = 291/878 (33.14%), Postives = 457/878 (52.05%), Query Frame = 1
Query: 388 NIVFIKVFATWETLCRYAEVLKIR---KQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKP-IPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA----RPEFVA-LIDKNYPKKTNLISGKEEPFIPF-WRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQII-SHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
+ F+K+ A W LCR AE LK++ K++ + E KTI L + D + + E +P + + P+S+ K++LFD+ D +FF + RS I V +IL R T C + GI L++ GV+ +AY LH+ D + +NVE N+R LLY+ W S ++ QP+D ++ YFGEKV YFAWLG YT L+P S++G+IV LY +I E+ Q NITMCPL + C +WK+S A + L DN ATV +V M++WA+ F+E WKR+Q++L Y W++ E E+E R E+ A +++K+ K++ KE + WR R P F +++ IA+ A + G+I+Y++ + + + M+ S ++ + + AT +++LV+I+ L VY +A WLT +E +T+ ++ +T K +LL+F+N+Y+ IFY+ F + P FRS M C GGCL++L +QL+ IM+GKQ+I +++ E +P++ ++ KL++ + +DR E + + +D + +EP +A L EY+EM+IQFGF+T+F+ +FPLAPL AL NN+ EIR DA KF LRRPVA R KDIGIW +I+ V +AV NA +I+F + +I +L+Y+ YS+NG ++GF + +LS N S+F L LG CRY +R PPW Y + +W +LAA++ FV +F+NLV + VIPD+P + + +E L E+ ++ + Q K Q
Sbjct: 152 GVGFVKIHAPWHVLCREAEFLKLKMPTKKVYHIS-ETRGLLKTINSVLQKITDPIQP-KVAEHRPQTTKRLSYPFSREKQHLFDL-TDRDSFFDSKTRSTI--------------------------VYEILKR---------------------TTC--------------TKAKYSMGITSLLANGVYSAAYPLHD----GDYEGDNVEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDENIPSMEMCDQRYNITMCPL--CDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESR---NKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRM---EECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRR-----------QSPSDREEYVKRKQRYEVD------FNLEP-----FAG----------LTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSE--HKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREE--QGKQQ 917
BLAST of Anoctamin vs. UniProt/SwissProt
Match: sp|Q5XXA6|ANO1_HUMAN (Anoctamin-1 OS=Homo sapiens OX=9606 GN=ANO1 PE=1 SV=1) HSP 1 Score: 390.578 bits (1002), Expect = 4.240e-117 Identity = 291/937 (31.06%), Postives = 462/937 (49.31%), Query Frame = 1
Query: 388 NIVFIKVFATWETLCRYAEVLKIR---KQMLSLGFEYDFNRK--TIFQRLFETFDFLNIFNINELKP-IPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA----RPEFVA-LIDKNYPKKTNLISGKEEPFIP--------------------------FWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQII-SHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPW-ETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMK-----------------NQHTSKLVADSFKKSA 3012
+ F+K+ A W LCR AE LK++ K+M + +K ++ Q++ + + E +P + + P+S+ K++LFD+ D +FF + RS I V +IL R T C + GI L++ GV+ +AY LH+ D + ENVE N+R LLY+ W ++ QP+D ++ YFGEK+ YFAWLG YT L+P S++GIIV LY +I E+ Q NITMCPL + C +WK+S A + L DN ATV +V M++WA+ F+E WKR+Q++L Y W++ E E+E R E+ A +++K+ K++ + ++ IP WR R P +++ IA+ A + G+I+Y++ + + M+ S ++ + + AT +++LV+I+ L VY +A WLT +E +T+ ++ + K +LL+F+N+Y+ IFY+ F + P FRS M C GGCL++L +QL+ IM+GKQ+I +++ E +P++ ++ KLKQ + D E + + +D + +EP +A L EY+EM+IQFGF+T+F+ +FPLAPL AL NN+ EIR DA KF LRRPVA R KDIGIW +I+ + +AV NA +I+F + +I +L+Y+ YS+NG ++GF + +LS N S+F L LG CRY +R PPW E KY+ + +W +LAA++ FV +F+NLV + VIPD+P + + +E L E+ ++ + + + N H +K DS A
Sbjct: 152 GVGFVKIHAPWNVLCREAEFLKLKMPTKKMYHINETRGLLKKINSVLQKITDPIQP----KVAEHRPQTMKRLSYPFSREKQHLFDL-SDKDSFFDSKTRSTI--------------------------VYEILKR---------------------TTC--------------TKAKYSMGITSLLANGVYAAAYPLHD----GDYNGENVEFNDRKLLYEEWARYGVFYKYQPIDLVRKYFGEKIGLYFAWLGVYTQMLIPASIVGIIVFLYGCATMDENIPSMEMCDQRHNITMCPL--CDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLKKESR--NKEKRRHIPEESTNKWKQRVKTAMAGVKLTDKVKLTWRDRFPAYLTNLVSIIFMIAVTFAIVLGVIIYRISMAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLIFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRM---EECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQ-----------QSPPDHEECVKRKQRYEVD------YNLEP-----FAG----------LTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAVRAKDIGIWYNILRGIGKLAVIINAFVISFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSENKYD---ISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQDKQQLLETWMEKERQKDEPPCNHHNTKACPDSLGSPA 976
BLAST of Anoctamin vs. TrEMBL
Match: A0A5K4FF50 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 682.945 bits (1761), Expect = 0.000e+0 Identity = 380/885 (42.94%), Postives = 556/885 (62.82%), Query Frame = 1
Query: 388 NIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNI--NELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ-GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATI-LDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSKLVAD-SFKKSAVIAKK 3027
N+VF+K+ A+WE + YAE LK+RK + L + + T +F+ DFL + N +KPI +T P+S +++YLFDIPE+ FFTA ER+L++++ILRR Y+ D E + + DI++ + DEN + S ++ +L ++ K + GI KLIS+G+F +AY LHE P N Y W S + +F+ QP+ +I+ YFGE VAFYFAWLG+YT WLLPI++LGIIV L+ ++ +D ++ E+ G NI MCPL C S C+FW L + R L+RL+D+ TVL AVVMS+WA +F EMWKR+QV LAY WNV+ +E D+P RPEF+AL+ K P+K N ++G EPFIPFWRR++P++ ++STVLL++ + LAFL G++LYKLV ++ HH+ + + + +I TGSL++LV I L LVY R+A LTD+EHHRTQ +YD+S+TLKLYLL+F+N YSSIFYI FIQ T +P +I+ GC G CL +L +QL IM+GKQI S I+E +P L +++ + ++ N +D + + + T L P + + I C AD + DPG RPLF EYLEM+IQ+GFIT+F+PAFPLAPL L NNLFEIR DA K RRPV +RV+ IGIW+SI++++A+IA+++NA +IAF T +ID+ +Y YS +G + GF +F+LSYM+++ F+ + TNETYCRY +R PPW + EPY +T +Y+ +LAAK +FV+ FE + + +I +PDVP ++R + E+ +TN IIL A++ Q+ + + D +F+K ++ ++
Sbjct: 151 NLVFVKLHASWEAMTYYAEYLKMRKPLRQLAYGWKLVNPTPKTSVFK--DFLKCLRMDKNIIKPIDTCYTWPFSMNRQYLFDIPENKDEFFTAVERALVLDHILRRTGYKS-EDLVNTEDLYEPSISDIVTSH------DLADEN------------SGSSNQANSLYVMATK--NLGITKLISDGIFSAAYPLHE-PAGSTLAPTEFNNRILLQRY--WASYKMLFKCQPISYIRYYFGEAVAFYFAWLGFYTAWLLPIAILGIIVFLFGLLDLKSDSIIHEVCDLGQNIYMCPL-CKSSKCKFWTLDTSCLRTKLMRLVDHEGTVLFAVVMSLWAVIFFEMWKRKQVSLAYRWNVYSLEPMDQPPRPEFMALLSKRCPRKVNSLTGYVEPFIPFWRRKVPIILVSFSTVLLTVILTLAFLVGVVLYKLVIKVILYRHHNPIVQSTAGMIATMTGSLVNLVTIFFLKLVYDRMATKLTDIEHHRTQVEYDNSLTLKLYLLQFVNYYSSIFYIAFIQGPTSAVPG-SEHILIQSTGCDQGDCLFELFIQLVIIMVGKQIFSFIQESLMPVLWKLFFKFR---SMKRSSNYADHNHNMNSINFNESNPVTTSLLPAKYSS--LSGRNILCRADFNMLDPGSRPLFNEYLEMMIQYGFITMFVPAFPLAPLFGLLNNLFEIRGDAKKLVNQYRRPVLERVQTIGIWLSIITVLASIAIRTNACIIAFTTQFIDRCVYRYTYSPDGTMKGFVNFTLSYMSTTRFD----------VPTNETYCRYDSYRSPPWSS--EPYVFTSIYYHVLAAKFIFVFTFETIATILTNVIMAAVPDVPKRVRRKIFHESNITNAIILNAEV--GNQQYNNAITKDVNFQKIVLLKQQ 988
BLAST of Anoctamin vs. TrEMBL
Match: A0A5K4FDS2 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 677.937 bits (1748), Expect = 0.000e+0 Identity = 380/904 (42.04%), Postives = 561/904 (62.06%), Query Frame = 1
Query: 388 NIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNI--NELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXY-------------------KHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ-GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATI-LDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSKLVAD-SFKKSAVIAKK 3027
N+VF+K+ A+WE + YAE LK+RK + L + + T +F+ DFL + N +KPI +T P+S +++YLFDIPE+ FFTA ER+L++++ILRR Y+ D E + + DI++ + DEN + S ++ +L ++ K + GI KLIS+G+F +AY LHE P + + +N Y ++W S + +F+ QP+ +I+ YFGE VAFYFAWLG+YT WLLPI++LGIIV L+ ++ +D ++ E+ G NI MCPL C S C+FW L + R L+RL+D+ TVL AVVMS+WA +F EMWKR+QV LAY WNV+ +E D+P RPEF+AL+ K P+K N ++G EPFIPFWRR++P++ ++STVLL++ + LAFL G++LYKLV ++ HH+ + + + +I TGSL++LV I L LVY R+A LTD+EHHRTQ +YD+S+TLKLYLL+F+N YSSIFYI FIQ T +P +I+ GC G CL +L +QL IM+GKQI S I+E +P L +++ + ++ N +D + + + T L P + + I C AD + DPG RPLF EYLEM+IQ+GFIT+F+PAFPLAPL L NNLFEIR DA K RRPV +RV+ IGIW+SI++++A+IA+++NA +IAF T +ID+ +Y YS +G + GF +F+LSYM+++ F+ + TNETYCRY +R PPW + EPY +T +Y+ +LAAK +FV+ FE + + +I +PDVP ++R + E+ +TN IIL A++ Q+ + + D +F+K ++ ++
Sbjct: 151 NLVFVKLHASWEAMTYYAEYLKMRKPLRQLAYGWKLVNPTPKTSVFK--DFLKCLRMDKNIIKPIDTCYTWPFSMNRQYLFDIPENKDEFFTAVERALVLDHILRRTGYKS-EDLVNTEDLYEPSISDIVTSH------DLADEN------------SGSSNQANSLYVMATK--NLGITKLISDGIFSAAYPLHE-PTKKLSLITLQQNIITPSCYGPAGSTLAPTEFNNRILLQRYWASYKMLFKCQPISYIRYYFGEAVAFYFAWLGFYTAWLLPIAILGIIVFLFGLLDLKSDSIIHEVCDLGQNIYMCPL-CKSSKCKFWTLDTSCLRTKLMRLVDHEGTVLFAVVMSLWAVIFFEMWKRKQVSLAYRWNVYSLEPMDQPPRPEFMALLSKRCPRKVNSLTGYVEPFIPFWRRKVPIILVSFSTVLLTVILTLAFLVGVVLYKLVIKVILYRHHNPIVQSTAGMIATMTGSLVNLVTIFFLKLVYDRMATKLTDIEHHRTQVEYDNSLTLKLYLLQFVNYYSSIFYIAFIQGPTSAVPG-SEHILIQSTGCDQGDCLFELFIQLVIIMVGKQIFSFIQESLMPVLWKLFFKFR---SMKRSSNYADHNHNMNSINFNESNPVTTSLLPAKYSS--LSGRNILCRADFNMLDPGSRPLFNEYLEMMIQYGFITMFVPAFPLAPLFGLLNNLFEIRGDAKKLVNQYRRPVLERVQTIGIWLSIITVLASIAIRTNACIIAFTTQFIDRCVYRYTYSPDGTMKGFVNFTLSYMSTTRFD----------VPTNETYCRYDSYRSPPWSS--EPYVFTSIYYHVLAAKFIFVFTFETIATILTNVIMAAVPDVPKRVRRKIFHESNITNAIILNAEV--GNQQYNNAITKDVNFQKIVLLKQQ 1009
BLAST of Anoctamin vs. TrEMBL
Match: A0A5K4FBC0 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 671.389 bits (1731), Expect = 0.000e+0 Identity = 379/904 (41.92%), Postives = 560/904 (61.95%), Query Frame = 1
Query: 388 NIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNI--NELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXY-------------------KHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ-GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATI-LDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSKLVAD-SFKKSAVIAKK 3027
N+VF+K+ A+WE + YAE LK+RK + L + ++F+ DFL + N +KPI +T P+S +++YLFDIPE+ FFTA ER+L++++ILRR Y+ D E + + DI++ + DEN + S ++ +L ++ K + GI KLIS+G+F +AY LHE P + + +N Y ++W S + +F+ QP+ +I+ YFGE VAFYFAWLG+YT WLLPI++LGIIV L+ ++ +D ++ E+ G NI MCPL C S C+FW L + R L+RL+D+ TVL AVVMS+WA +F EMWKR+QV LAY WNV+ +E D+P RPEF+AL+ K P+K N ++G EPFIPFWRR++P++ ++STVLL++ + LAFL G++LYKLV ++ HH+ + + + +I TGSL++LV I L LVY R+A LTD+EHHRTQ +YD+S+TLKLYLL+F+N YSSIFYI FIQ T +P +I+ GC G CL +L +QL IM+GKQI S I+E +P L +++ + ++ N +D + + + T L P + + I C AD + DPG RPLF EYLEM+IQ+GFIT+F+PAFPLAPL L NNLFEIR DA K RRPV +RV+ IGIW+SI++++A+IA+++NA +IAF T +ID+ +Y YS +G + GF +F+LSYM+++ F+ + TNETYCRY +R PPW + EPY +T +Y+ +LAAK +FV+ FE + + +I +PDVP ++R + E+ +TN IIL A++ Q+ + + D +F+K ++ ++
Sbjct: 151 NLVFVKLHASWEAMTYYAEYLKMRKPLRQLVNPTP--KTSVFK------DFLKCLRMDKNIIKPIDTCYTWPFSMNRQYLFDIPENKDEFFTAVERALVLDHILRRTGYKS-EDLVNTEDLYEPSISDIVTSH------DLADEN------------SGSSNQANSLYVMATK--NLGITKLISDGIFSAAYPLHE-PTKKLSLITLQQNIITPSCYGPAGSTLAPTEFNNRILLQRYWASYKMLFKCQPISYIRYYFGEAVAFYFAWLGFYTAWLLPIAILGIIVFLFGLLDLKSDSIIHEVCDLGQNIYMCPL-CKSSKCKFWTLDTSCLRTKLMRLVDHEGTVLFAVVMSLWAVIFFEMWKRKQVSLAYRWNVYSLEPMDQPPRPEFMALLSKRCPRKVNSLTGYVEPFIPFWRRKVPIILVSFSTVLLTVILTLAFLVGVVLYKLVIKVILYRHHNPIVQSTAGMIATMTGSLVNLVTIFFLKLVYDRMATKLTDIEHHRTQVEYDNSLTLKLYLLQFVNYYSSIFYIAFIQGPTSAVPG-SEHILIQSTGCDQGDCLFELFIQLVIIMVGKQIFSFIQESLMPVLWKLFFKFR---SMKRSSNYADHNHNMNSINFNESNPVTTSLLPAKYSS--LSGRNILCRADFNMLDPGSRPLFNEYLEMMIQYGFITMFVPAFPLAPLFGLLNNLFEIRGDAKKLVNQYRRPVLERVQTIGIWLSIITVLASIAIRTNACIIAFTTQFIDRCVYRYTYSPDGTMKGFVNFTLSYMSTTRFD----------VPTNETYCRYDSYRSPPWSS--EPYVFTSIYYHVLAAKFIFVFTFETIATILTNVIMAAVPDVPKRVRRKIFHESNITNAIILNAEV--GNQQYNNAITKDVNFQKIVLLKQQ 1003
BLAST of Anoctamin vs. TrEMBL
Match: U6JHY6 (Anoctamin OS=Echinococcus granulosus OX=6210 GN=EGR_10154 PE=3 SV=1) HSP 1 Score: 659.833 bits (1701), Expect = 0.000e+0 Identity = 401/994 (40.34%), Postives = 572/994 (57.55%), Query Frame = 1
Query: 169 MFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQD--------DGCKVYPFKENSYYDNDSVGSPSHRSN--------------------------IVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTI-FQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILH---------------ERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDK-NYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRL-----VSTVKRYKLKQA---EEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
MFF D R+IDFVLVY+ + ++ KR FL +L ++ +EIE+++ G + FK+ + + DS+G PS ++ +VF+K+ A+W T+ R AEVL+ RK + E R+T + FE D +IF + P + P+S+++ LFDIPED FF ERSL+V+Y+L+R + PV D +T+E D + + +PF ++ + VD GI LI++ VF +A+ LH E + E E N R LL W S++N+ + QPLD+++LYFGE +AFYFAWLG YTYWL+P+ + G++V + + S+D +V ++ +G+ MCP CS RGC+FW L+ + + L+DN +TVL A+ MSIWA+LF+E WKR Q L + WNV +E DEP RPEF+AL+ K Y NLI+G+EEP IPFW R++P ++S+V+L + + LA L G+I Y+L+ +L+ H + IS+M+ IIT T S ++LV I L LVY ++AV LTDME+HRTQS+YD S+TLKLYLL+F+N YSSIFYI FIQ T +P RS I+ GC G CL L +QLA IM+GKQ ++ + E LP + V R ++ EK+ ME+ +E K T D + P L C A+ L D G RPLF EYLEMLIQ+GFIT+F+PAFP+APL AL NN+FE+R+DA KF R LRRPV +R IGIW I++ ++ +A+++NA LIAF + Y+ ++ YI+ YS N L G+ DF+LSYMN+S F+ T L N TYCRY FR PP T +PY+YTPVYW +LA K FV++FEN+ A+IA IPD+P L I + EA + NE++L+ + ++ K +
Sbjct: 45 MFFRDRKRRIDFVLVYKAEETDTVSKSKRFAFLSALAEQMIEIEVENCQGEILAKTGPQKPGFKDEAGFLLDSMGQPSSDADAVRGYAQTGDQEDRNPLETAILANDDLVFVKLHASWSTMIRVAEVLQFRKPLKQAKVERLEMRETAKCSKCFE-LDPKSIFKL------PSPYRAPFSRTRMNLFDIPEDYDNFFNPTERSLVVDYVLKRTGTCECTGPGTCPPVKGGD---------DASPHYVTEE--------DVEYERKTRARPFDVKLAEHGKVDLGIDHLINDKVFIAAFPLHDPSEELMNLLKQKEGEVAGSSRPKTEQTEVNMRTLLNDQWGSLKNMIKFQPLDYVRLYFGESIAFYFAWLGLYTYWLIPVGIFGLLVFCLSAIDISDDRIVDDVCNRGSEFVMCP-TCSQRGCKFWLLNASCLATKMTHLVDNISTVLFAIFMSIWATLFMENWKRYQNVLGHRWNVQHLEPVDEPPRPEFLALLKKRGYKTTVNLITGREEPSIPFWSRKLPCTVLSFSSVILGMVLCLAALIGVIFYRLIVNTLLLQHENVFISSMAGIITTVTSSCINLVCIFILKLVYDKIAVKLTDMENHRTQSEYDDSLTLKLYLLQFVNFYSSIFYIAFIQGTTAGLPGDRSIP-IRSSGCDNGNCLFQLFIQLAIIMVGKQFLNFVTENTLPPIKRCLVVYFAHRRSMRDRGALAEKVSGAQYGSMESGALE---KTPLTSGDGR--------PILYNCRANFTLLDGGSRPLFEEYLEMLIQYGFITMFVPAFPVAPLFALLNNMFELRTDAKKFLRLLRRPVRKRENGIGIWFGILTAISALAIRTNACLIAFTSEYLVRMTYILYYSPNRTLEGYLDFTLSYMNASRFDLT---PKDEVLLKNSTYCRYRDFREPP--TAPDPYAYTPVYWHVLAVKFAFVFIFENVALALTALIARCIPDMPQNLTIISRHEARVVNELLLQNEEIEDKEK 996
BLAST of Anoctamin vs. TrEMBL
Match: A0A087W085 (Anoctamin OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000175600 PE=3 SV=1) HSP 1 Score: 657.522 bits (1695), Expect = 0.000e+0 Identity = 399/994 (40.14%), Postives = 572/994 (57.55%), Query Frame = 1
Query: 169 MFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQD--------DGCKVYPFKENSYYDNDSVGSPSHRSN--------------------------IVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTI-FQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILH---------------ERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIY-QGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDK-NYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRL-----VSTVKRYKLKQA---EEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
MFF D R+IDFVLVY+ + ++ KR FL +L ++ +EIE+++ G + FK+ + + DS+G PS ++ +VF+K+ A+W T+ R AEVL+ RK + E R+T + FE D +IF + P + P+S+++ LFDIPED FF ERSL+V+Y+L+R + P+ D +T+E D + + +PF ++ + VD GI LI++ +F +A+ LH E + E E N R LL W S++N+ + QPLD+++LYFGE +AFYFAWLG YTYWL+P+ + G++V + + S+D +V ++ +G+ MCP CS RGC+FW L+ + + L+DN +TVL A+ MSIWA+LF+E WKR Q L + WNV +E DEP RPEF+AL+ K Y NLI+G+EEP IPFW R++P ++S+V+L + + LA L G+I Y+L+ +L+ H + IS+M+ IIT T S ++LV I L LVY ++AV LTDME+HRTQS+YD S+TLKLYLL+F+N YSSIFYI FIQ T +P RS I+ GC G CL L +QLA IM+GKQ ++ + E LP + V R ++ EK+ ME+ +E K T D + P L C A+ L D G RPLF EYLEMLIQ+GFIT+F+PAFP+APL AL NN+FE+R+DA KF R LRRPV +R IGIW I++ ++ +A+++NA LIAF + Y+ ++ YI+ YS N L G+ DF+LSYMN+S F+ T L N TYCRY FR PP T +PY+YTPVYW +LA K FV++FEN+ A+IA IPD+P L I + EA + NE++L+ + ++ K +
Sbjct: 45 MFFRDRKRRIDFVLVYKAEETDIVSKSKRFAFLSALAEQMIEIEVENCQGEILAKTGPQKPGFKDEAGFLLDSMGQPSSDADAVRGYAQTGDQEDRNPLETAILANDDLVFVKLHASWSTMIRVAEVLQFRKPLKQAKVERLEMRETAKCSKCFE-LDPKSIFKL------PSPYRAPFSRTRMNLFDIPEDYDNFFNPTERSLVVDYVLKRTGTCECTALGTCPPIKGGD---------DASPHYVTEE--------DVEYERKTRARPFDVKLAEHGKVDLGIDHLINDKIFIAAFPLHDPSEELMNLLKQKEGEVAGSSRPKTEQTEVNMRTLLNDQWGSLKNMIKFQPLDYVRLYFGESIAFYFAWLGLYTYWLIPVGIFGLLVFCLSAIDISDDRIVDDVCNRGSEFVMCP-TCSQRGCKFWLLNASCLATKMTHLVDNISTVLFAIFMSIWATLFMENWKRYQNVLGHRWNVQHLEPVDEPPRPEFLALLKKRGYKTTVNLITGREEPSIPFWSRKLPCTVLSFSSVILGMVLCLAALIGVIFYRLIVNTLLLQHENVFISSMAGIITTVTSSCINLVCIFILKLVYDKIAVKLTDMENHRTQSEYDDSLTLKLYLLQFVNFYSSIFYIAFIQGTTASLPGDRSIP-IRSSGCDNGNCLFQLFIQLAIIMVGKQFLNFVTENTLPPIKRCLVVYFAHRRSMRDRGALAEKVSGAQYGSMESGALE---KTPLTSGDGR--------PTLYNCRANFTLLDGGSRPLFEEYLEMLIQYGFITMFVPAFPVAPLFALLNNMFELRTDAKKFLRLLRRPVRKRENGIGIWFGILTAISALAIRTNACLIAFTSEYLVRMTYILYYSPNRTLEGYLDFTLSYMNASRFDLT---PKDELLLKNSTYCRYRDFREPP--TAPDPYAYTPVYWHVLAVKFAFVFIFENVALALTALIARCIPDMPQNLTIISRHEARVVNELLLQNEEIEDKEK 996
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:103044829]) HSP 1 Score: 415.231 bits (1066), Expect = 6.199e-128 Identity = 309/979 (31.56%), Postives = 483/979 (49.34%), Query Frame = 1
Query: 115 ILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYD---FNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFL--NGFTDFSLSYMNSSEFNA--TLYGGHKLTLGTNE--TYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
+ D+ ++ + + ++F DG R+IDFVL Y ++R Q +R + +++ K GLE+E++D + S F+K+ A WE L YA+VLKI+ + F+ + NR+ LF + + ++P P FT+P+ KSK+ F I +D T F + R+ IV YIL R Y R ++N+N+ DK GI +L++ G + SA+ LH+ + +ER LY++W ++ QPL+ I+ Y+GEK+ YFAWLG+YT L +L+G+I +Y + I KEI G I MCPL + C FWKL+ + + L DN ATV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K ++ N ++ + EP+ P + + +TVL I++ +A + G+I Y+L F S+M+ + + + S+IT + T S ++ VIIM L Y RVA+W+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P + S ++ C GGCL++L+ QL +M GKQ+ +I+E LP L + K + E L + R D L + LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ E+R DA K RRPVA + IG W I++M+A +V +NA ++AF + I +L+Y+ Y L G+ + SLS N S+ ++ T G N T CRY +R+PP +YE YS+T +W ILAAK+ F+ + E++V++ ++A +IPDVP ++ +KRE L E + ++ ++K Q
Sbjct: 38 VFQLDDATVDKQHQSKDSVYFRDGKRRIDFVLSYIEDKEDR-KQERRKLYEENMQKAGLELELEDK-------------------TESEDGKTYFLKIHAPWEVLATYADVLKIK-----VPFKVNDIPENREIPVSWLFTPL----LLPDHVMRPDPDYFTSPFDKSKDDFFLI-DDKETLFPPSTRNRIVYYILSRCFYNR--------------------------------DDNINK------------DKR-------------GIKRLLNNGTYTSAFPLHD-CRYWTKSRDTHCESERYSLYRYWARFFRFYKEQPLNLIRKYYGEKIGIYFAWLGFYTEMLFYAALVGLICFIYGAATYDDVIWTKEICDDDIGGQIVMCPL--CDKKCGFWKLNSTCNSTWQSHLFDNTATVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCTSRRRNKVTQEMEPYFPV-TSKCARACLSGATVLFWISLIIASIIGVIAYRLAVFAALASVMKDSPTNKMELVGSLITPQLATSVTASCINFVIIMILNFFYERVAIWITDMEVPKTHLEYENKLTIKMFLFQFVNYYSSCFYVAFFKGKFVGYPGKYTYMFNGSGLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALLPWLKNWWSSRKARSHPESLYS-------------------------------------RWEQDYDLQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALMNNILEVRVDAWKLTTQFRRPVAAKAHSIGAWDEILNMIAVFSVVTNAFIVAFTSDMIPRLVYLYAYHPGTELTMQGYVNNSLSIFNISQLHSDNTPDPGENPAWFNNSVITTCRYRDYRYPP-GHEYE-YSHTMQFWHILAAKMAFIIIMEHVVFVVKFLVAWLIPDVPSDVQARVKRERYLVQEYLHNYEVDKLKKQ 883
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:103044829]) HSP 1 Score: 413.305 bits (1061), Expect = 1.561e-127 Identity = 306/972 (31.48%), Postives = 483/972 (49.69%), Query Frame = 1
Query: 136 DINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYD---FNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFL--NGFTDFSLSYMNSSEFNA--TLYGGHKLTLGTNE--TYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
+++ + + ++F DG R+IDFVL Y ++R Q +R + +++ K GLE+E++D + S F+K+ A WE L YA+VLKI+ + F+ + NR+ LF + + ++P P FT+P+ KSK+ F I +D T F + R+ IV YIL R Y R ++N+N+ DK GI +L++ G + SA+ LH+ + +ER LY++W ++ QPL+ I+ Y+GEK+ YFAWLG+YT L +L+G+I +Y + I KEI G I MCPL + C FWKL+ + + L DN ATV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K ++ N ++ + EP+ P + + +TVL I++ +A + G+I Y+L F S+M+ + + + S+IT + T S ++ VIIM L Y RVA+W+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P + S ++ C GGCL++L+ QL +M GKQ+ +I+E LP L + K + E L ++ + + + L + LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ E+R DA K RRPVA + IG W I++M+A +V +NA ++AF + I +L+Y+ Y L G+ + SLS N S+ ++ T G N T CRY +R+PP +YE YS+T +W ILAAK+ F+ + E++V++ ++A +IPDVP ++ +KRE L E + ++ ++K Q
Sbjct: 16 EVDKQHQSKDSVYFRDGKRRIDFVLSYIEDKEDR-KQERRKLYEENMQKAGLELELEDK-------------------TESEDGKTYFLKIHAPWEVLATYADVLKIK-----VPFKVNDIPENREIPVSWLFTPL----LLPDHVMRPDPDYFTSPFDKSKDDFFLI-DDKETLFPPSTRNRIVYYILSRCFYNR--------------------------------DDNINK------------DKR-------------GIKRLLNNGTYTSAFPLHD-CRYWTKSRDTHCESERYSLYRYWARFFRFYKEQPLNLIRKYYGEKIGIYFAWLGFYTEMLFYAALVGLICFIYGAATYDDVIWTKEICDDDIGGQIVMCPL--CDKKCGFWKLNSTCNSTWQSHLFDNTATVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCTSRRRNKVTQEMEPYFPV-TSKCARACLSGATVLFWISLIIASIIGVIAYRLAVFAALASVMKDSPTNKMELVGSLITPQLATSVTASCINFVIIMILNFFYERVAIWITDMEVPKTHLEYENKLTIKMFLFQFVNYYSSCFYVAFFKGKFVGYPGKYTYMFNGSGLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALLPWLKNWWSSRKARSHPESLYSRWEQDYD-------------------------------------LQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALMNNILEVRVDAWKLTTQFRRPVAAKAHSIGAWDEILNMIAVFSVVTNAFIVAFTSDMIPRLVYLYAYHPGTELTMQGYVNNSLSIFNISQLHSDNTPDPGENPAWFNNSVITTCRYRDYRYPP-GHEYE-YSHTMQFWHILAAKMAFIIIMEHVVFVVKFLVAWLIPDVPSDVQARVKRERYLVQEYLHNYEVDKLKKQ 854
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:103044829]) HSP 1 Score: 413.305 bits (1061), Expect = 1.714e-127 Identity = 306/972 (31.48%), Postives = 483/972 (49.69%), Query Frame = 1
Query: 136 DINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYD---FNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFL--NGFTDFSLSYMNSSEFNA--TLYGGHKLTLGTNE--TYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
+++ + + ++F DG R+IDFVL Y ++R Q +R + +++ K GLE+E++D + S F+K+ A WE L YA+VLKI+ + F+ + NR+ LF + + ++P P FT+P+ KSK+ F I +D T F + R+ IV YIL R Y R ++N+N+ DK GI +L++ G + SA+ LH+ + +ER LY++W ++ QPL+ I+ Y+GEK+ YFAWLG+YT L +L+G+I +Y + I KEI G I MCPL + C FWKL+ + + L DN ATV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K ++ N ++ + EP+ P + + +TVL I++ +A + G+I Y+L F S+M+ + + + S+IT + T S ++ VIIM L Y RVA+W+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P + S ++ C GGCL++L+ QL +M GKQ+ +I+E LP L + K + E L ++ + + + L + LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ E+R DA K RRPVA + IG W I++M+A +V +NA ++AF + I +L+Y+ Y L G+ + SLS N S+ ++ T G N T CRY +R+PP +YE YS+T +W ILAAK+ F+ + E++V++ ++A +IPDVP ++ +KRE L E + ++ ++K Q
Sbjct: 11 EVDKQHQSKDSVYFRDGKRRIDFVLSYIEDKEDR-KQERRKLYEENMQKAGLELELEDK-------------------TESEDGKTYFLKIHAPWEVLATYADVLKIK-----VPFKVNDIPENREIPVSWLFTPL----LLPDHVMRPDPDYFTSPFDKSKDDFFLI-DDKETLFPPSTRNRIVYYILSRCFYNR--------------------------------DDNINK------------DKR-------------GIKRLLNNGTYTSAFPLHD-CRYWTKSRDTHCESERYSLYRYWARFFRFYKEQPLNLIRKYYGEKIGIYFAWLGFYTEMLFYAALVGLICFIYGAATYDDVIWTKEICDDDIGGQIVMCPL--CDKKCGFWKLNSTCNSTWQSHLFDNTATVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCTSRRRNKVTQEMEPYFPV-TSKCARACLSGATVLFWISLIIASIIGVIAYRLAVFAALASVMKDSPTNKMELVGSLITPQLATSVTASCINFVIIMILNFFYERVAIWITDMEVPKTHLEYENKLTIKMFLFQFVNYYSSCFYVAFFKGKFVGYPGKYTYMFNGSGLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALLPWLKNWWSSRKARSHPESLYSRWEQDYD-------------------------------------LQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALMNNILEVRVDAWKLTTQFRRPVAAKAHSIGAWDEILNMIAVFSVVTNAFIVAFTSDMIPRLVYLYAYHPGTELTMQGYVNNSLSIFNISQLHSDNTPDPGENPAWFNNSVITTCRYRDYRYPP-GHEYE-YSHTMQFWHILAAKMAFIIIMEHVVFVVKFLVAWLIPDVPSDVQARVKRERYLVQEYLHNYEVDKLKKQ 849
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:103044829]) HSP 1 Score: 412.535 bits (1059), Expect = 8.246e-127 Identity = 306/971 (31.51%), Postives = 482/971 (49.64%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYD---FNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFL--NGFTDFSLSYMNSSEFNA--TLYGGHKLTLGTNE--TYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
++ + + ++F DG R+IDFVL Y ++R Q +R + +++ K GLE+E++D + S F+K+ A WE L YA+VLKI+ + F+ + NR+ LF + + ++P P FT+P+ KSK+ F I +D T F + R+ IV YIL R Y R ++N+N+ DK GI +L++ G + SA+ LH+ + +ER LY++W ++ QPL+ I+ Y+GEK+ YFAWLG+YT L +L+G+I +Y + I KEI G I MCPL + C FWKL+ + + L DN ATV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K ++ N ++ + EP+ P + + +TVL I++ +A + G+I Y+L F S+M+ + + + S+IT + T S ++ VIIM L Y RVA+W+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P + S ++ C GGCL++L+ QL +M GKQ+ +I+E LP L + K + E L ++ + + + L + LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ E+R DA K RRPVA + IG W I++M+A +V +NA ++AF + I +L+Y+ Y L G+ + SLS N S+ ++ T G N T CRY +R+PP +YE YS+T +W ILAAK+ F+ + E++V++ ++A +IPDVP ++ +KRE L E + ++ ++K Q
Sbjct: 60 VDKQHQSKDSVYFRDGKRRIDFVLSYIEDKEDR-KQERRKLYEENMQKAGLELELEDK-------------------TESEDGKTYFLKIHAPWEVLATYADVLKIK-----VPFKVNDIPENREIPVSWLFTPL----LLPDHVMRPDPDYFTSPFDKSKDDFFLI-DDKETLFPPSTRNRIVYYILSRCFYNR--------------------------------DDNINK------------DKR-------------GIKRLLNNGTYTSAFPLHD-CRYWTKSRDTHCESERYSLYRYWARFFRFYKEQPLNLIRKYYGEKIGIYFAWLGFYTEMLFYAALVGLICFIYGAATYDDVIWTKEICDDDIGGQIVMCPL--CDKKCGFWKLNSTCNSTWQSHLFDNTATVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCTSRRRNKVTQEMEPYFPV-TSKCARACLSGATVLFWISLIIASIIGVIAYRLAVFAALASVMKDSPTNKMELVGSLITPQLATSVTASCINFVIIMILNFFYERVAIWITDMEVPKTHLEYENKLTIKMFLFQFVNYYSSCFYVAFFKGKFVGYPGKYTYMFNGSGLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALLPWLKNWWSSRKARSHPESLYSRWEQDYD-------------------------------------LQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALMNNILEVRVDAWKLTTQFRRPVAAKAHSIGAWDEILNMIAVFSVVTNAFIVAFTSDMIPRLVYLYAYHPGTELTMQGYVNNSLSIFNISQLHSDNTPDPGENPAWFNNSVITTCRYRDYRYPP-GHEYE-YSHTMQFWHILAAKMAFIIIMEHVVFVVKFLVAWLIPDVPSDVQARVKRERYLVQEYLHNYEVDKLKKQ 897
BLAST of Anoctamin vs. Ensembl Cavefish
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:103044829]) HSP 1 Score: 413.305 bits (1061), Expect = 1.364e-126 Identity = 308/971 (31.72%), Postives = 480/971 (49.43%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYD---FNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFL--NGFTDFSLSYMNSSEFNA--TLYGGHKLTLGTNE--TYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
++ + + ++F DG R+IDFVL Y ++R Q +R + +++ K GLE+E++D + S F+K+ A WE L YA+VLKI+ + F+ + NR+ LF + + ++P P FT+P+ KSK+ F I +D T F + R+ IV YIL R Y R ++N+N+ DK GI +L++ G + SA+ LH+ + +ER LY++W ++ QPL+ I+ Y+GEK+ YFAWLG+YT L +L+G+I +Y + I KEI G I MCPL + C FWKL+ + + L DN ATV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K ++ N ++ + EP+ P + + +TVL I++ +A + G+I Y+L F S+M+ + + + S+IT + T S ++ VIIM L Y RVA+W+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P + S ++ C GGCL++L+ QL +M GKQ+ +I+E LP L + K + E L + R D L + LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ E+R DA K RRPVA + IG W I++M+A +V +NA ++AF + I +L+Y+ Y L G+ + SLS N S+ ++ T G N T CRY +R+PP +YE YS+T +W ILAAK+ F+ + E++V++ ++A +IPDVP ++ +KRE L E + ++ ++K Q
Sbjct: 104 VDKQHQSKDSVYFRDGKRRIDFVLSYIEDKEDR-KQERRKLYEENMQKAGLELELEDK-------------------TESEDGKTYFLKIHAPWEVLATYADVLKIK-----VPFKVNDIPENREIPVSWLFTPL----LLPDHVMRPDPDYFTSPFDKSKDDFFLI-DDKETLFPPSTRNRIVYYILSRCFYNR--------------------------------DDNINK------------DKR-------------GIKRLLNNGTYTSAFPLHD-CRYWTKSRDTHCESERYSLYRYWARFFRFYKEQPLNLIRKYYGEKIGIYFAWLGFYTEMLFYAALVGLICFIYGAATYDDVIWTKEICDDDIGGQIVMCPL--CDKKCGFWKLNSTCNSTWQSHLFDNTATVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCTSRRRNKVTQEMEPYFPV-TSKCARACLSGATVLFWISLIIASIIGVIAYRLAVFAALASVMKDSPTNKMELVGSLITPQLATSVTASCINFVIIMILNFFYERVAIWITDMEVPKTHLEYENKLTIKMFLFQFVNYYSSCFYVAFFKGKFVGYPGKYTYMFNGSGLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALLPWLKNWWSSRKARSHPESLYS-------------------------------------RWEQDYDLQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALMNNILEVRVDAWKLTTQFRRPVAAKAHSIGAWDEILNMIAVFSVVTNAFIVAFTSDMIPRLVYLYAYHPGTELTMQGYVNNSLSIFNISQLHSDNTPDPGENPAWFNNSVITTCRYRDYRYPP-GHEYE-YSHTMQFWHILAAKMAFIIIMEHVVFVVKFLVAWLIPDVPSDVQARVKRERYLVQEYLHNYEVDKLKKQ 941
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ano11 (anoctamin 11 [Source:ZFIN;Acc:ZDB-GENE-090313-245]) HSP 1 Score: 353.214 bits (905), Expect = 7.153e-107 Identity = 269/859 (31.32%), Postives = 435/859 (50.64%), Query Frame = 1
Query: 376 SHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXX-XXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWL-GYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ-GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYK-LVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIP-SFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEII 2937
+ NI ++K+ A W+ L YAE + +R + + + +RL + NI + + + + + KSK F + TFF++ +R +I V++IL+ P G + K + G+ +++ + +F +A+ LHE + + R +LYK+W F+ QPLDHI+ YFGEK+AFYFAWL G+YT WLLP +L+G++V + ND VKEI + G++ MCPL C + C+ W LS+ + + + D T+ ++ +S+W+ FLE WKR+ L YHW + +E+E RPE+ AL + N I+G++EP+ R P + L I++ L FL +ILY+ L+S S+ + + ++ ++ I TG+L+ LV+I+ + +Y+ +A +LT E HRTQS++++++T+K+++ + +N YSS Y+GF + P ++ + + CP GGCL++L+ QL IM+GKQI+++++E LP + +R KL + K + Q ++ W+ + EL L + G LF EYLEM++QFGFITIF+ AFPLAPL AL NN EIR DA KF RR V +RV+ IG+W I+S +A I++ SNA LIAF + ++ +L+Y Y + L+G+ DF+L+Y A N T CRY FR + + T YW++LA ++ FV +FE+LV+L I +I DV L++ +KRE L + +
Sbjct: 51 GEKINIHYLKLHAPWQVLQCYAEEINLRMPLQI----HPMTKTHFSERLLKFLHLPNIMHADLPRQPQEYYNCFFRKSKLDRFLGSDHPETFFSSTQRHVI--------------------------VKEILN-------------------------TTPYGKR---------KKAEVGLDRMLRDHIFSAAFPLHEGSYDGTYLGLAPEQMTRRQVLYKYWARWGCWFKYQPLDHIREYFGEKIAFYFAWLLGFYTCWLLPPALVGLVVFFVGWLTLHNDSTVKEICENGSHYQMCPL-CDT--CKVWNLSNTCNMAKMGHIFDYPGTIFYSIFISLWSVSFLEYWKRKSSSLTYHWGC-TLVHEEERPRPEYAALAPL---IRENPITGEKEPYFSE-DDRTPRTVTGSIVLFLMISVVLIFLVTVILYQALLSISIFKTG-NWFLATQATNIANMTGTLVFLVLIVIMARLYTTIADYLTRWEMHRTQSEHENALTVKVFIFQLVNYYSSPVYVGFFKGKFNGYPGNYGNLFGTRNENCPQGGCLIELAQQLFIIMVGKQILNNLQELILPIIQQWRQRRKLDEIRGKRLVQE------------------------LNIWEDDYEL--------LHNTG---LFDEYLEMVMQFGFITIFVMAFPLAPLFALMNNWVEIRLDARKFVCRYRRSVVERVRGIGVWFDILSGLAHISIISNAFLIAFTSDFLPRLLYRYTYKSS--LDGYVDFTLAYAPRDYVVA------------NHTMCRYRDFRD-------QDGNLTLFYWKLLAVRLSFVIIFEHLVFLVARAIDWLIVDVAPDLKVKIKREEYLAKQAL 780
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000399.1 (pep scaffold:Pmarinus_7.0:GL477564:2673:43187:-1 gene:ENSPMAG00000000358.1 transcript:ENSPMAT00000000399.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 312.383 bits (799), Expect = 4.785e-92 Identity = 217/614 (35.34%), Postives = 340/614 (55.37%), Query Frame = 1
Query: 913 GIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ-GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVA-LIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQ--------PFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQII-SHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRY 2721
GI LI+ GV+ SA+ LH+ +L E N+R LL+ W S ++ QPL+ IK YFGEK+ YFAWLG YT L+P S++GI V LY + DI E G NITMCP + C++ +LS+ R L DN TVL ++ M++WA+ FLE WKR + +L Y W+++ ME ++ RPE+ A L++K L + E + WR ++P S +L + ++ A + G+I Y++ L+ + + S I AT L++LVII+ L +Y +A WLT +E +T+ ++++ + K ++L+F+N+Y+SI Y+ F++ + +P++R C GGCL +L+LQL IM+GKQ++ +++ E +P+ + + RY K+ K ++ E D +E+ KD +++EP G P EY+EM+IQFGF+T+F+ +FPLAP AL NN+ EIR D KF LRRP A R KDIG W I++ VA +AV NA++IA + ++ +LIY+ YS +G L G+ +++LSY N S++ + + +N T CRY
Sbjct: 228 GITSLIANGVYTSAFPLHD------GELHGSEVNDRKLLHDEWASYNKFYKYQPLELIKKYFGEKIGLYFAWLGVYTQMLIPPSVVGIAVFLYGWLAAEKDIPGFETCVIGQNITMCP--TCDKLCKYKRLSENCDRVRSSHLFDNITTVLFSIFMALWATTFLEHWKRIETRLNYTWDLNGMEESEQQLRPEYEAKLLEKKRKVDIKLAKTQTEEYGLSWRDQLPSFLINCSCILFMVLLSFAIVVGMIFYRITMNVLLANSTDPYLKSNRSAIIMATAFLINLVIILILDEIYGSIARWLTVIEVPKTEEEFETRLIFKSFMLKFVNSYTSIIYVAFLKGRITGYPKKYYYILPNYRMEE------CAPGGCLQELALQLCIIMLGKQLLQNNLFEVGIPK-IKKLCRYLHKKDHHK---KASHLKEEDGMELGCKD------------YELEP------------FTGLNP---EYMEMVIQFGFVTLFVASFPLAPFFALLNNVIEIRLDGKKFVTELRRPTAVRAKDIGTWYVILNGVAKLAVIINALVIALTSDFVPRLIYMYFYSPDGSLRGYVNYTLSYFNVSDYAPG------IIIESNYTLCRY 790
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ano3 (anoctamin 3 [Source:ZFIN;Acc:ZDB-GENE-111031-3]) HSP 1 Score: 259.225 bits (661), Expect = 7.872e-76 Identity = 188/538 (34.94%), Postives = 293/538 (54.46%), Query Frame = 1
Query: 1387 ASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIG-FIQPFT-QPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGY--------SENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
A++FLE WKR++ +LAY W++ D E E+E RP+F A + ++ N ISGK EP+ F + + + S + L I + + + G+++Y++V S+ + + S TA TG ++ VIIM L ++Y +VAV+LT++E RT++++++S TLK++L +F+N SSIFY+ F+ FT P + + + C GCL+DL +Q+ IM+ KQ ++ E P L + + KL+ EME ++A+ + W+ D L LF EYLEM++QFGF TIF+ AFPLAPLLAL NN+ EIR DA KF RR +A R KDIG+W I+ + ++V +NA +IA + +I +L+Y Y SE L G+ + SLS N S + + + YC Y +R P + EPY +T +W +LAA++ F+ +FE+LVY IIA +IPD+P L+ M RE L E++ +ADL ++ +
Sbjct: 7 ATVFLEFWKRRKSELAYEWDLLDWEEEEEEIRPQFEAKYSQK--ERLNAISGKPEPYQSF-HDKCSRLVVSASGIFLMICLVIVAVFGVVVYRVVMLSVFTTSELVFVRSYSQFFTAGTGVCINFVIIMLLNMIYEKVAVFLTNLEQCRTEAEWENSFTLKMFLFQFVNLNSSIFYVAFFLGRFTGHPGKYEKLFHIWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGFPLLQNWWAKKKLQH-----------EMETTEDDMAL-----------LPQWE---------QDYSLQQINTYGLFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRSLACRAKDIGVWYGILEGIGVVSVITNAFVIAVTSDFIPRLVYFYQYGPCARMRESERDCLEGYVNSSLSVFNPSTYCQII-----------KCYCIYRDYREAPSTHREEPYKFTLQFWHVLAARLAFIIVFEHLVYFIKLIIAYMIPDIPKGLKERMHREKFLVQEMVHEADLDALRKE 499
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ano11 (anoctamin 11 [Source:ZFIN;Acc:ZDB-GENE-090313-245]) HSP 1 Score: 223.402 bits (568), Expect = 1.525e-64 Identity = 150/445 (33.71%), Postives = 242/445 (54.38%), Query Frame = 1
Query: 1606 TVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEM--LIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNE 2931
++L +++ + FL +I+Y+ + +M + + ++ I + S+++L++I+ + VYS +A LT E HRTQ+QY+ + T K+++ +F+N YSS FY+ F + P ++ + C GGCL++L+ QL IM+GKQ+I++I+E+ P++ + +++ KL + S E+ R W+ + ELI C LF EYLEM ++QFGFITIF+ AFPLAPL AL NN E+R DA KF RRPVA+R +DIG+W +I+ + I+V NA LIAF + ++ KL+Y Y N L G+ +F+LSY + N T+CRY +R + +T YW++LA ++ F+ FE++V+ +I ++PDVP+ L I MKRE L +
Sbjct: 4 VIILMLSVVMIFLVTVIMYRGIVSVVMYKTGNAVLMTQAANIANISSSMVNLILILVMGQVYSSLAEQLTKWEMHRTQTQYEDAFTFKVFVFQFVNFYSSPFYVAFFKGRFVGYPGHYKKLLGTRNEDCGPGGCLIELAQQLIIIMVGKQVINNIQEFVFPKIKAWLQKRKLAA-----LRGSHVAPESSR-------------------WEEDYELIPC-----------EGLFEEYLEMGPVLQFGFITIFVAAFPLAPLFALLNNWVELRLDAQKFVCEYRRPVAERAQDIGVWFNILDALVNISVIINAFLIAFTSNFLPKLLYQYTYDRN--LRGYINFTLSYAPDD------------YVVNNYTHCRYKDYRD-------QDGKHTLFYWKLLAIRLGFIIAFEHVVFFISRMIDWLVPDVPESLEIKMKRERYLAKQ 392
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Match: ano11 (anoctamin 11 [Source:ZFIN;Acc:ZDB-GENE-090313-245]) HSP 1 Score: 219.935 bits (559), Expect = 2.976e-63 Identity = 152/444 (34.23%), Postives = 243/444 (54.73%), Query Frame = 1
Query: 1615 LSIAMALAFLGGIILYK-LVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALP-RLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEM--LIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNE 2931
L +++ + FL +I+Y+ +VS + + ++ ++ ++ I + S+++L++I+ + VYS +A LT E HRTQ+QY+ + T K+++ +F+N YSS FY+ F + P ++ + C GGCL++L+ QL IM+GKQ+I++I+E+ P L R ++ + L + A + V P+ W+ + ELI C LF EYLEM ++QFGFITIF+ AFPLAPL AL NN E+R DA KF RRPVA+R +DIG+W +I+ + I+V NA LIAF + ++ KL+Y Y N L G+ +F+LSY + N T+CRY +R + +T YW++LA ++ F+ FE++V+ +I ++PDVP+ L I MKRE L +
Sbjct: 1 LQLSVVMIFLVTVIMYRGIVSVVMYKTGNAVLMTQQAANIANISSSMVNLILILVMGQVYSSLAEQLTKWEMHRTQTQYEDAFTFKVFVFQFVNFYSSPFYVAFFKGRFVGYPGHYKKLLGTRNEDCGPGGCLIELAQQLIIIMVGKQVINNIQEFVFPLTLPPPAPRSCRRKIKAWLQKRKLAALRGSHVA-----------PES-SRWEEDYELIPCEG-----------LFEEYLEMGPVLQFGFITIFVAAFPLAPLFALLNNWVELRLDAQKFVCEYRRPVAERAQDIGVWFNILDALVNISVIINAFLIAFTSNFLPKLLYQYTYDRN--LRGYINFTLSYAPDD------------YVVNNYTHCRYKDYRD-------QDGKHTLFYWKLLAIRLGFIIAFEHVVFFISRMIDWLVPDVPESLEIKMKRERYLAKQ 400
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO49855 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7REV0]) HSP 1 Score: 424.476 bits (1090), Expect = 3.568e-133 Identity = 309/923 (33.48%), Postives = 454/923 (49.19%), Query Frame = 1
Query: 199 DFVLVYERINDNRTNQI--KRMTFLKSLLKEGLEIE---IQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNI--VYTSNDIVVKEIYQGTNIT--MCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEII 2937
DFVLVYE + Q R FL++L K LE E QD K++ FIK+ WE L YAE L R L +G N +R+ E F NIF P P FT + +K F +D ++FT ER+ R +Y +++E AP G + + + GI +L+ EGVF + Y LH +L ++W + QPLDHI+ YFGEK+ YFAWLG YT WL+ S LG++V LY + + +S++ E+ T MCPL GC++W L + + L DN +TV AV +S W+ FLE WKR++V LAYHW+V + E E E RP F AL + N ++G EP P +R P +F + V +++ L F+ G+I+YKL+ + + + S + I +TG+ ++L IIM L+ VY RVA+ LT E HRTQ++Y+ S+T K+++ +F N YSSIFY+ F + P +R ++ C GGCL++L+ QL IM+GKQ+I++++E A+P + +KR K ++++ + P+ W+ + EL+ LF EYLEM++QFGFITIF+ AFPLAP AL NN+FEIR D+ KF +RRP+A R +DIGIW +I+ VA IAV SNA LIAF +T++ KL+Y SEN L G+T+++L++ N TL CRY FR P +T YW +LA ++ FV +FE++V+ M +I +++PD+P +L I +KREA L + +
Sbjct: 1 DFVLVYEEGEKDPPPQSIEYRQKFLENLAKSQLEFEEEITQDKKVKLH-----------------------FIKIHVPWEVLLFYAEELSFRAP-LEVGLNTKVNWS---ERMLEKFHMPNIFKEEVPNPPPDFFTCTFQANKLNKFIGSDDPDSYFTDVERT--------RVAY--------------EILET-----------------------------APYGKR---------QRGEIGIERLVEEGVFKAGYPLHVNCYLNQQGYSPFLIYVSQVLKEYWGRWGKWLKYQPLDHIRDYFGEKIGIYFAWLGQYTAWLIMPSFLGLLVFLYGVATINSSDNKPALEVCDNPPWTFRMCPLCDEELGCQYWDLHISCKSGKIAYLFDNASTVFFAVFVSFWSCFFLEYWKRKEVTLAYHWDVLEYEEEAERPRPTFAALAPT---VERNPVTGILEPHFPD-EKRKPRLFSGIAIVFTMVSLVLVFMVGVIVYKLLVYRPLARNPSTRAYALQ--IANSTGAFVNLTIIMILSRVYERVALALTHWEMHRTQTEYEDSLTFKVFVFQFANFYSSIFYVAFFKGKLVGYPGHYRRLFGLRQEECSPGGCLMELAQQLVIIMVGKQMINNVQEIAIPLVKQWIKRKKRGTSKDE------------------------IKPR----WEQDFELVENEG-----------LFQEYLEMILQFGFITIFVAAFPLAPFFALANNIFEIRIDSDKFVCEVRRPIADRAQDIGIWFNILDSVAKIAVISNAFLIAFTSTFLPKLLYRYAVSENFSLEGYTNYTLAWAPR---NTTL------------KPCRYQEFRDPEGH-------FTVFYWHLLALRLGFVIMFEHVVFFVMRLIDMMVPDIPTELEIIIKREAYLAKQAL 769
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO48050 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7RKU9]) HSP 1 Score: 375.17 bits (962), Expect = 1.565e-114 Identity = 233/697 (33.43%), Postives = 368/697 (52.80%), Query Frame = 1
Query: 883 EDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGT---NITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISG--KEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQ---PFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTN---------ETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATL 2922
+ + D GI +LI +G + +AY LH P ++ + W F+ QP D IK YFG ++ YFAWLG+Y L+P ++ G+ V +Y I ++ VK+I MCPL + C +W L + DN +TV+ A+ MSIWA++FLE WKR+Q LAY W++ E ++E RPEF+ + K + G P + +R+ V+ S V IA+ L+ + G+++Y+ + + + + + + T+ T ++L+L+ I L VY ++A++LT+ E+ RT++ + S T K+YL +F+NNY+SIFYI F + P R + + GC +GGCL+DL +QL IM+G+QII +I E A+P L +K +K++QA+++ + I W+V+ Y N L + LF EYLE+++QFGF+T+F+ AFPLAPL AL N +FE+R DA+ F RRP +R +DIG W+SIM +A I+V NA +IAF + +I +L+Y YS +G L G+ + SLSY N +++ G + G N YCRY G+ P + PY + YW ++AA++ FV+ F+ +VY +A ++PD P L + +KR+ L
Sbjct: 162 QRFGEERFDQGIKRLIHQGTYTAAYPLHSGPEEVSRKGHPSNERQKLR--RDWARFGRWFKYQPYDAIKDYFGTEIGLYFAWLGFYAAMLVPAAIFGVAVFIYGIAQSTIFPPVKDICNKAYEAEFYMCPL--CDKQCPYWSLVVNCPYAIWVHAFDNDSTVVFAIFMSIWATVFLEFWKRRQAVLAYEWHMMHYEDQEEQPRPEFIVTVTTLKKDKASQWGGMVPHVPKLQQYRKLAGVV----SLVAFMIALVLSAVVGVVIYRASVYGSLMAYPDPQVRKQAKMTTSITAAILNLICINLLKFVYEKLAMFLTEWENPRTETDFKDSFTYKMYLFQFVNNYASIFYIAFFKLNLVIGTPGNYRRFAGEYRLDGCSSGGCLMDLCIQLVIIMVGQQIIGNITEIAIPGL---LKWWKMRQAQKEAFEE-------------------------IPRWEVD------YKLNTLPE---HHLFWEYLEVVLQFGFVTMFVAAFPLAPLFALVNAVFELRVDAINFVCQFRRPTPRRAQDIGAWMSIMQGLANISVLVNAFVIAFTSDFIPRLVYTTAYSPDGTLRGYLNNSLSYFNVKDYDRMGAIGAREKPGDNYAYTVLNYTREYCRYPGYHEPEF-----PYEVSKQYWHVIAARLAFVFAFQYIVYAVTKFVAWLVPDRPRHLELRIKRQEYL 808
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO49854 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7REU8]) HSP 1 Score: 356.295 bits (913), Expect = 1.249e-108 Identity = 238/687 (34.64%), Postives = 374/687 (54.44%), Query Frame = 1
Query: 907 DFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYF-----GEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNI--VYTSNDIVVKEI--YQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQ-PFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEII 2937
+ GI++L+ EG + +AY LH P D+ N+ + ++W ++ QPLDHI+ + G K+A YFAWLG YT WL+ S++G+IV +Y+ + N++ E+ + MCP C GC W L + SR L DN TV ++ +S WA LFLE WKR++V + + W+V + E E+E RP F AL + N I+G EP+ P +R P + + + +++ + F+ G+I+YKL+ + ++ + + ++ + TG++L+L+IIM L+ VY ++A+ L E HRTQ++Y+ ++TLK+++ +F N YSSIFYI F + F ++ + ++ C GGCL++L+ QLA IMIGKQ+I +I+E +P L +K K + +++ E IK + W+ + EL+ DN LF EYLEM+IQ+GFIT+F+ AFPLAP AL NN+FEIR DA KF LRR A R +DIG+W +I+ + +AV SNA LIAF + ++ KL+Y S N L G+ ++SL++ + T CRY GFR E ++T +W +L ++VFV LFE+ V+ +I V +PD+P L +KRE + + +
Sbjct: 70 EIGISRLVDEGTYSAAYPLHVGPHGPDEMKLNMRQVLK----EYWARWGAWYKYQPLDHIRQAYEREGGGLKIAIYFAWLGQYTAWLIMPSVVGLIVFIYSFATINGPNNMPALEMCDHPAWTYPMCP-NCEV-GCSVWDLKKSCSRAKHAYLFDNPMTVAYSIFVSFWAVLFLEFWKRKEVTIGHAWDVLEFESEEERPRPTFAALAPA---VERNPITGLLEPYFPD-EKRSPRVLSGIAIICGMVSLVMLFMVGVIVYKLLV--IHPLYKNPNLQPYANQFASGTGAVLNLIIIMILSRVYEKLALLLNHWEMHRTQTEYEDNLTLKVFIFQFTNFYSSIFYIAFFKGKFVGYPGNYGTIFGLRNEECSPGGCLIELAQQLAVIMIGKQVIGNIQEVFIPELKKYLKNRKRQNSKD---------------EDEIKPR-----------WEADYELL----DN-------EGLFQEYLEMVIQYGFITLFVAAFPLAPFFALANNVFEIRIDADKFVNDLRRSTADRAQDIGVWFTILDSITKLAVISNAFLIAFTSEFLPKLLYAGVVSPNQSLEGYLNYSLAWAPPN---------------TTSQPCRYRGFRD-------EDGNFTRFFWHLLTMRLVFVILFEHFVFGISTLIDVAVPDIPRALEDTIKREKYVATQAL 685
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO48048 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7RKU7]) HSP 1 Score: 353.214 bits (905), Expect = 6.020e-106 Identity = 242/697 (34.72%), Postives = 377/697 (54.09%), Query Frame = 1
Query: 913 GIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTN-ITMCPLMCSSRGCRFWKLSDA---YSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVL-LSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNM-------SSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQP---FTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYS---ENGFLNGFTDFSLSYMNSSEFNATLYGGHKL----TLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEII 2937
G+ +L+ EG + + Y LH+ + + N+ + L K W SM+ IF+ QPLD +K Y+G +A YFAWLG+YT L+P++++G++V +Y I ++ V++ N MCPL R C +W L Y+R + DN TV +A+ S+WA+LFLE WKR+Q LAY W+V + E E+E RPEF A K N+I+GK EP IP RR + + +V+ I + +A + G+++Y+ F+ + + TI S IIT+ T +L++L+ I L L Y+R+AVWLT+ E+ RT++ Y+ S T K+YL +F+N Y+SIFYI F + P R + + GC A GC L+L +Q+ IM+G+QII +I E A+P +++ +K K E K N+ + E D + ++P+ LF EYLE+++Q+GF+T+FI AFPLAPL +L N++ EIR DA+ F RRP +DIG W I+ + ++V NA +++F + +I KL+Y + Y+ G L G+ + SLS +N + G L L ET+CRY G+ + P+ + YW ++AA++ FV++F+ VY + IA +PD P L + KRE LT +I+
Sbjct: 188 GLKQLVYEGAYCANYPLHDGDDDVIGESSYPSNDRQRLK-KDWSSMKRIFKYQPLDTVKGYYGTAIALYFAWLGFYTAMLIPLAIVGLLVFIYGIGSSATSPTVRDACDTNNKFYMCPL--CDRTCSYWDLVSTTCMYAR--VTHFFDNDGTVFLAIFTSVWATLFLEFWKRRQAVLAYEWHVANFEEEEEQIRPEFAATAPT---LKKNIITGKLEPHIP--RRTLYQRYGAIGSVVAFMILLVIAAVIGVVVYRAAVFAALSGNSDSTIRARYAMIAIGSRIITSMTAALINLLCINILKLFYNRLAVWLTNWENPRTKTDYEDSFTYKMYLFQFVNTYASIFYIAFFKSEIVVGTPGRYKRIAGKYRLEGCSAQGCFLELCVQMLIIMVGQQIIGNITEVAIPAIMTWIKERK----EPK--NKQLPQFEQD--------------------YNLQPQ-------------EEHNLFWEYLEIVLQYGFVTMFIAAFPLAPLFSLLNSIVEIRVDAINFVSQFRRPDTMIAEDIGAWYRILETLTRLSVLINAFVLSFTSEFIPKLVYKLKYAPEGSEGTLEGYLNNSLSAINVTVMEEWEPGTAPLNPTKNLNYTETFCRYKGYHDNTY-----PFKFNKQYWHVIAARLAFVFVFQYTVYAITSFIAWAVPDQPRSLELRSKREEHLTKKIL 830
BLAST of Anoctamin vs. Ensembl Nematostella
Match: EDO48049 (Anoctamin [Source:UniProtKB/TrEMBL;Acc:A7RKU8]) HSP 1 Score: 329.717 bits (844), Expect = 1.914e-98 Identity = 223/689 (32.37%), Postives = 368/689 (53.41%), Query Frame = 1
Query: 910 FGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTN---ITMCPLMCSSRGCRFWKLSDAYSRF-FLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAM-ALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQP---FTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSEN----GFLNGFTDFSLSYMNSSEFNATLYGGHKL-TLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEII 2937
G+ +L+ EG + Y LH+ + + +R + W S F+ QP D IK YFG ++ YFAWLG+YT L+P+++ G++V +Y IV + V+++ N MCPL + C +W L+ + ++ DN TV +AVV SIWA+LFLE+WKR+Q LA W+V D E E+E RPEF A + K N ++G +EP++P +R + + T ++++ + + +A + G+++Y+ F+ + TI + +T+ T ++++L+ I L VYS +AV LT+ E+ RT++ Y+ S T K+YL +F+N Y+SIFYI F + P R + + GC GC L+L +QL IM+G+QII +I E A+P L+ +KR K +PK + +L + D L+ G +F EYLE+++Q+GF+T+F+ AFPLAPL +L N++ EIR DA+ F R RRP A R +DIG W I+ + ++V NA +++F + ++ +L+Y + Y+ + G L G+ + SL+ + + L ++ T CRY G+ + P+ + YW ++A ++ FV++F+ VY F +A +IPD L + +KRE L ++I
Sbjct: 116 LGLKQLLYEGAYIGNYPLHDGDDEVKEGETISNDRQRLR--RDWASFGRCFKYQPYDAIKSYFGTEIGLYFAWLGFYTGMLVPLAIAGLVVFIYGIVDATMSPPVRDVCDSKNEGRWIMCPL--CDKQCSYWDLAKTTCAYAYVTHFFDNSLTVALAVVSSIWATLFLELWKRRQASLANKWHVSDFE-EEETIRPEFAATAPR---LKKNPVTGLQEPYVP--KRTLYQRYGTTGSIIVMMCLLVIAAVVGVVIYRAAVFASLSGSEILTIRMRARTVTSITAAIINLICINILKFVYSWLAVKLTNWENPRTRTDYEDSFTYKMYLFQFVNTYASIFYIAFFKSGLVIGTPGRYKRIAGKYRLDGCSEQGCFLELCIQLIIIMVGQQIIGNITEIAIPALMEWMKRRK--------------------------------EPK-------DKQLPQYQQDFDLNSLGEHNMFWEYLEVVLQYGFVTMFVAAFPLAPLFSLLNSIVEIRVDAINFVRQFRRPDAARAEDIGAWSRILEGLTKLSVLVNAFVLSFTSDFVPRLLYRLKYAPDRDSGGSLEGYLNNSLAVFEVAYLEPGTEPENPLQSINYTVTTCRYPGY-----HSNTAPFKFNKQYWHVIAGRLAFVFVFQYTVYAFTQFLAWLIPDRTKTLDLKIKREQYLAKQLI 750
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:101164640]) HSP 1 Score: 421.394 bits (1082), Expect = 1.110e-130 Identity = 315/976 (32.27%), Postives = 480/976 (49.18%), Query Frame = 1
Query: 136 DINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNI-FNINE--LKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLN--GFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
+I ++ + +FF DGVR+IDFVL Y D Q +R F +L+K GLE+EI+D S S F+K+ A WE L YA+VLKI+ F I ++L+ F + E ++P P FT P+ KSK F I ED FF + R+ IV Y ILSR + I DE + GI +L++ + +A+ LH+ + +++R LYK+W + F+ QPL+ I+ Y+GEK+ YFAWLG+YT L +++G + +Y ++ ++ KEI G NI MCPL + C FWKL+ + + L DN TV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K +K N I+ + EP++P + + + +TVLL I++ +A + G+I Y+L + S+M+ + + + IT + T S ++ VIIM L L+Y RVAVW+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P F S ++ C GGCL++L+ QL +M GKQ+ +I+E +P L++ K + E L + R D+ L G LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ EIR DA K RRPVA + IG W I++ +A ++V +NA ++AF + I +L+Y+ Y G +N G+ + SLS N SE N G + L ++ T CRY +R+PP YS+T +W ILAAK+ F+ + E++V+L +A +IPDVP ++ +KRE L E + ++ ++K Q + +
Sbjct: 2 EIQKQQQSKDSVFFRDGVRRIDFVLSYVDDKDGERKQERRRVFEANLVKVGLELEIEDK-------------------SESDDRKTYFVKIHAPWEVLATYADVLKIK---------VPFKANDIPDNSEMPMNWLSTPFRLPEPIMQPEPDFFTAPFDKSKSDFFLI-EDRDKFFLPSTRNRIVYY--------------------------ILSR-----CSYIRDECGERDKK--------------------------GIKRLLNNSTYTAAFPLHDS-RYWTKSKDVNCDSDRYNLYKYWAGFFSFFKEQPLNLIRKYYGEKIGLYFAWLGFYTEMLFFAAVVGTLCFVYGVLTYDDNEWSKEICSDEIGGNIVMCPL--CDKKCGFWKLNTTCNSSWQSHLFDNVGTVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCNSRKLNRITQEMEPYLPI-TSKCARICLSGATVLLWISLIIACIIGVIAYRLAVYAAFASIMKDSPTSNLEVVGPYITPQLATSVTASCINFVIIMILNLMYERVAVWITDMEIPKTHLEYENKLTVKMFLFQFVNYYSSCFYVAFFKGKFVGYPGDYAYMFGKWSKLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALVPWLMNWWASRKARSYPESLYS-------------------------------------RWEQDHDLQGFGQLGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNIIEIRVDAWKLTTQFRRPVAAKAHCIGAWEEILNGMAVLSVVTNAFIVAFTSDMIPRLVYMYAYQPEGEMNMKGYINNSLSVFNISEIPLPNRPEDGENPLWFNSSITTCRYRDYRNPP--GHENQYSHTMQFWHILAAKLAFIIIMEHVVFLVKYFVAWMIPDVPSDVKARVKRERYLVQEYLHNYEVEKLKIQLSQQ 845
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:101164640]) HSP 1 Score: 421.394 bits (1082), Expect = 3.877e-130 Identity = 315/975 (32.31%), Postives = 479/975 (49.13%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNI-FNINE--LKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLN--GFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
I ++ + +FF DGVR+IDFVL Y D Q +R F +L+K GLE+EI+D S S F+K+ A WE L YA+VLKI+ F I ++L+ F + E ++P P FT P+ KSK F I ED FF + R+ IV Y ILSR + I DE + GI +L++ + +A+ LH+ + +++R LYK+W + F+ QPL+ I+ Y+GEK+ YFAWLG+YT L +++G + +Y ++ ++ KEI G NI MCPL + C FWKL+ + + L DN TV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K +K N I+ + EP++P + + + +TVLL I++ +A + G+I Y+L + S+M+ + + + IT + T S ++ VIIM L L+Y RVAVW+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P F S ++ C GGCL++L+ QL +M GKQ+ +I+E +P L++ K + E L + R D+ L G LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ EIR DA K RRPVA + IG W I++ +A ++V +NA ++AF + I +L+Y+ Y G +N G+ + SLS N SE N G + L ++ T CRY +R+PP YS+T +W ILAAK+ F+ + E++V+L +A +IPDVP ++ +KRE L E + ++ ++K Q + +
Sbjct: 47 IQKQQQSKDSVFFRDGVRRIDFVLSYVDDKDGERKQERRRVFEANLVKVGLELEIEDK-------------------SESDDRKTYFVKIHAPWEVLATYADVLKIK---------VPFKANDIPDNSEMPMNWLSTPFRLPEPIMQPEPDFFTAPFDKSKSDFFLI-EDRDKFFLPSTRNRIVYY--------------------------ILSR-----CSYIRDECGERDKK--------------------------GIKRLLNNSTYTAAFPLHDS-RYWTKSKDVNCDSDRYNLYKYWAGFFSFFKEQPLNLIRKYYGEKIGLYFAWLGFYTEMLFFAAVVGTLCFVYGVLTYDDNEWSKEICSDEIGGNIVMCPL--CDKKCGFWKLNTTCNSSWQSHLFDNVGTVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCNSRKLNRITQEMEPYLPI-TSKCARICLSGATVLLWISLIIACIIGVIAYRLAVYAAFASIMKDSPTSNLEVVGPYITPQLATSVTASCINFVIIMILNLMYERVAVWITDMEIPKTHLEYENKLTVKMFLFQFVNYYSSCFYVAFFKGKFVGYPGDYAYMFGKWSKLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALVPWLMNWWASRKARSYPESLYS-------------------------------------RWEQDHDLQGFGQLGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNIIEIRVDAWKLTTQFRRPVAAKAHCIGAWEEILNGMAVLSVVTNAFIVAFTSDMIPRLVYMYAYQPEGEMNMKGYINNSLSVFNISEIPLPNRPEDGENPLWFNSSITTCRYRDYRNPPGHEN--QYSHTMQFWHILAAKLAFIIIMEHVVFLVKYFVAWMIPDVPSDVKARVKRERYLVQEYLHNYEVEKLKIQLSQQ 889
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:101164640]) HSP 1 Score: 421.779 bits (1083), Expect = 1.239e-129 Identity = 315/975 (32.31%), Postives = 479/975 (49.13%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNI-FNINE--LKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLN--GFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
I ++ + +FF DGVR+IDFVL Y D Q +R F +L+K GLE+EI+D S S F+K+ A WE L YA+VLKI+ F I ++L+ F + E ++P P FT P+ KSK F I ED FF + R+ IV Y ILSR + I DE + GI +L++ + +A+ LH+ + +++R LYK+W + F+ QPL+ I+ Y+GEK+ YFAWLG+YT L +++G + +Y ++ ++ KEI G NI MCPL + C FWKL+ + + L DN TV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K +K N I+ + EP++P + + + +TVLL I++ +A + G+I Y+L + S+M+ + + + IT + T S ++ VIIM L L+Y RVAVW+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P F S ++ C GGCL++L+ QL +M GKQ+ +I+E +P L++ K + E L + R D+ L G LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ EIR DA K RRPVA + IG W I++ +A ++V +NA ++AF + I +L+Y+ Y G +N G+ + SLS N SE N G + L ++ T CRY +R+PP YS+T +W ILAAK+ F+ + E++V+L +A +IPDVP ++ +KRE L E + ++ ++K Q + +
Sbjct: 107 IQKQQQSKDSVFFRDGVRRIDFVLSYVDDKDGERKQERRRVFEANLVKVGLELEIEDK-------------------SESDDRKTYFVKIHAPWEVLATYADVLKIK---------VPFKANDIPDNSEMPMNWLSTPFRLPEPIMQPEPDFFTAPFDKSKSDFFLI-EDRDKFFLPSTRNRIVYY--------------------------ILSR-----CSYIRDECGERDKK--------------------------GIKRLLNNSTYTAAFPLHDS-RYWTKSKDVNCDSDRYNLYKYWAGFFSFFKEQPLNLIRKYYGEKIGLYFAWLGFYTEMLFFAAVVGTLCFVYGVLTYDDNEWSKEICSDEIGGNIVMCPL--CDKKCGFWKLNTTCNSSWQSHLFDNVGTVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCNSRKLNRITQEMEPYLPI-TSKCARICLSGATVLLWISLIIACIIGVIAYRLAVYAAFASIMKDSPTSNLEVVGPYITPQLATSVTASCINFVIIMILNLMYERVAVWITDMEIPKTHLEYENKLTVKMFLFQFVNYYSSCFYVAFFKGKFVGYPGDYAYMFGKWSKLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALVPWLMNWWASRKARSYPESLYS-------------------------------------RWEQDHDLQGFGQLGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNIIEIRVDAWKLTTQFRRPVAAKAHCIGAWEEILNGMAVLSVVTNAFIVAFTSDMIPRLVYMYAYQPEGEMNMKGYINNSLSVFNISEIPLPNRPEDGENPLWFNSSITTCRYRDYRNPPGHEN--QYSHTMQFWHILAAKLAFIIIMEHVVFLVKYFVAWMIPDVPSDVKARVKRERYLVQEYLHNYEVEKLKIQLSQQ 949
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:101164640]) HSP 1 Score: 421.394 bits (1082), Expect = 4.469e-129 Identity = 315/971 (32.44%), Postives = 477/971 (49.12%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNI-FNINE--LKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLN--GFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
I ++ + +FF DGVR+IDFVL Y D Q +R F +L+K GLE+EI+D S S F+K+ A WE L YA+VLKI+ F I ++L+ F + E ++P P FT P+ KSK F I ED FF + R+ IV Y ILSR + I DE + GI +L++ + +A+ LH+ + +++R LYK+W + F+ QPL+ I+ Y+GEK+ YFAWLG+YT L +++G + +Y ++ ++ KEI G NI MCPL + C FWKL+ + + L DN TV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K +K N I+ + EP++P + + + +TVLL I++ +A + G+I Y+L + S+M+ + + + IT + T S ++ VIIM L L+Y RVAVW+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P F S ++ C GGCL++L+ QL +M GKQ+ +I+E +P L++ K + E L + R D+ L G LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ EIR DA K RRPVA + IG W I++ +A ++V +NA ++AF + I +L+Y+ Y G +N G+ + SLS N SE N G + L ++ T CRY +R+PP YS+T +W ILAAK+ F+ + E++V+L +A +IPDVP ++ +KRE L E + ++ ++K Q
Sbjct: 158 IQKQQQSKDSVFFRDGVRRIDFVLSYVDDKDGERKQERRRVFEANLVKVGLELEIEDK-------------------SESDDRKTYFVKIHAPWEVLATYADVLKIK---------VPFKANDIPDNSEMPMNWLSTPFRLPEPIMQPEPDFFTAPFDKSKSDFFLI-EDRDKFFLPSTRNRIVYY--------------------------ILSR-----CSYIRDECGERDKK--------------------------GIKRLLNNSTYTAAFPLHDS-RYWTKSKDVNCDSDRYNLYKYWAGFFSFFKEQPLNLIRKYYGEKIGLYFAWLGFYTEMLFFAAVVGTLCFVYGVLTYDDNEWSKEICSDEIGGNIVMCPL--CDKKCGFWKLNTTCNSSWQSHLFDNVGTVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCNSRKLNRITQEMEPYLPI-TSKCARICLSGATVLLWISLIIACIIGVIAYRLAVYAAFASIMKDSPTSNLEVVGPYITPQLATSVTASCINFVIIMILNLMYERVAVWITDMEIPKTHLEYENKLTVKMFLFQFVNYYSSCFYVAFFKGKFVGYPGDYAYMFGKWSKLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALVPWLMNWWASRKARSYPESLYS-------------------------------------RWEQDHDLQGFGQLGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNIIEIRVDAWKLTTQFRRPVAAKAHCIGAWEEILNGMAVLSVVTNAFIVAFTSDMIPRLVYMYAYQPEGEMNMKGYINNSLSVFNISEIPLPNRPEDGENPLWFNSSITTCRYRDYRNPP--GHENQYSHTMQFWHILAAKLAFIIIMEHVVFLVKYFVAWMIPDVPSDVKARVKRERYLVQEYLHNYEVEKLKIQ 996
BLAST of Anoctamin vs. Ensembl Medaka
Match: ano5a (anoctamin 5 [Source:NCBI gene;Acc:101164640]) HSP 1 Score: 421.394 bits (1082), Expect = 5.722e-129 Identity = 315/971 (32.44%), Postives = 479/971 (49.33%), Query Frame = 1
Query: 139 INDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNI-FNINE--LKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQ---GTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPA--RPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSF----SLMRMHHSRTISNMSSIIT-----AATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPS-----FRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLN--GFTDFSLSYMNSSEF---NATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQ 2970
I ++ + +FF DGVR+IDFVL Y D Q +R F +L+K GLE+EI+D S S F+K+ A WE L YA+VLKI+ F I ++L+ F + E ++P P FT P+ KSK F I ED FF + R+ IV Y ILSR + I DE + GI +L++ + +A+ LH+ + +++R LYK+W + F+ QPL+ I+ Y+GEK+ YFAWLG+YT L +++G + +Y ++ ++ KEI G NI MCPL + C FWKL+ + + L DN TV A+ M IW +LFLE WKR+Q +L Y W++ D E E + RPE+ K +K N I+ + EP++P + + + +TVLL I++ +A + G+I Y+L + S+M+ + + + IT + T S ++ VIIM L L+Y RVAVW+TDME +T +Y++ +T+K++L +F+N YSS FY+ F + P F S ++ C GGCL++L+ QL +M GKQ+ +I+E +P L++ K + E L ++ W+ D+ L G LF EYLEM+IQFGFIT+F+ +FPLAPLLAL NN+ EIR DA K RRPVA + IG W I++ +A ++V +NA ++AF + I +L+Y+ Y G +N G+ + SLS N SE N G + L ++ T CRY +R+PP YS+T +W ILAAK+ F+ + E++V+L +A +IPDVP ++ +KRE L E + ++ ++K Q
Sbjct: 172 IQKQQQSKDSVFFRDGVRRIDFVLSYVDDKDGERKQERRRVFEANLVKVGLELEIEDK-------------------SESDDRKTYFVKIHAPWEVLATYADVLKIK---------VPFKANDIPDNSEMPMNWLSTPFRLPEPIMQPEPDFFTAPFDKSKSDFFLI-EDRDKFFLPSTRNRIVYY--------------------------ILSR-----CSYIRDECGERDKK--------------------------GIKRLLNNSTYTAAFPLHDS-RYWTKSKDVNCDSDRYNLYKYWAGFFSFFKEQPLNLIRKYYGEKIGLYFAWLGFYTEMLFFAAVVGTLCFVYGVLTYDDNEWSKEICSDEIGGNIVMCPL--CDKKCGFWKLNTTCNSSWQSHLFDNVGTVFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQLRPEYET---KCNSRKLNRITQEMEPYLPI-TSKCARICLSGATVLLWISLIIACIIGVIAYRLAVYAAFASIMKDSPTSNLEVVGPYITPQLATSVTASCINFVIIMILNLMYERVAVWITDMEIPKTHLEYENKLTVKMFLFQFVNYYSSCFYVAFFKGKFVGYPGDYAYMFGKWSKLRNEECDPGGCLIELTTQLVIVMTGKQVWGNIQEALVPWLMNWWASRKARSYPESLYSR----------------------------WE---------QDHDLQGFGQLGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNIIEIRVDAWKLTTQFRRPVAAKAHCIGAWEEILNGMAVLSVVTNAFIVAFTSDMIPRLVYMYAYQPEGEMNMKGYINNSLSVFNISEIPLPNRPEDGENPLWFNSSITTCRYRDYRNPP--GHENQYSHTMQFWHILAAKLAFIIIMEHVVFLVKYFVAWMIPDVPSDVKARVKRERYLVQEYLHNYEVEKLKIQ 1010
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000033728.1 (SMESG000033728.1) HSP 1 Score: 1713.74 bits (4437), Expect = 0.000e+0 Identity = 865/867 (99.77%), Postives = 865/867 (99.77%), Query Frame = 1
Query: 97 MSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLG 2697
MSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFA EDISNKTVDFGIAKLISEGVFDSAYILHERPLNEDDDLENVENNERNLLYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITIFIPAFPLAPLLALTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLT G
Sbjct: 1 MSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFAFEDISNKTVDFGIAKLISEGVFDSAYILHERPLNEDDDLENVENNERNLLYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITIFIPAFPLAPLLALTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTWG 867 HSP 2 Score: 92.8189 bits (229), Expect = 9.472e-19 Identity = 43/43 (100.00%), Postives = 43/43 (100.00%), Query Frame = 2
Query: 2693 WGLMKRIADILDFGIRHGKRNMNLIHIHLSIGKYSLQKLYSFT 2821
WGLMKRIADILDFGIRHGKRNMNLIHIHLSIGKYSLQKLYSFT
Sbjct: 866 WGLMKRIADILDFGIRHGKRNMNLIHIHLSIGKYSLQKLYSFT 908
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000033732.1 (SMESG000033732.1) HSP 1 Score: 936.406 bits (2419), Expect = 0.000e+0 Identity = 507/969 (52.32%), Postives = 678/969 (69.97%), Query Frame = 1
Query: 142 NDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETF---DFLNIFNIN--ELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTW------KVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSKLVADSFKKSAV 3015
N+ +K N GMFF D VR+IDFVLVY+++ D+ + KR++F++SL+ EGLEIEIQ+ G ++ ++ ++D + S N+VF+K+ A+W+TLCRYAE++KIRK +++ G +Y++ IFQ FE F DF IF+I+ LKPI +G+T P+SK K YLFDIP + + FFT ERSLIVNYIL+R Y K +P+ ++ + + VE+ I + N + TN +G ++FGIAKLISE VF++A+ILHE+ + + + N R LLYKHW ++ NIFR QPLDHI+LYFGE VAFYFAWLG+YT+WL PI+L G+ V++YN+ DI+++EI + NITMCP+ C C WKLS Y +F+LIRL+DNGATV++A V+S+W LFLEMW+R+Q LA+ WNVHD+E EDEP RPE+ AL++KNY + N ISGKEEPFI FW+ +P+ T ST+L SI + LA L GI + K + +L+R+ + +S +S TAA GSL+SL+ I+ LT VYSRVAVWLTD+EHHRTQSQYD+SVT KLY+LEFINNYSS+FYIGF P+T+ P S SMIKP+GC G CLLDLS+QL FI++GKQIIS+I +++PRL S++K Y++K+A+ K I + I D I + +C D + K++ EL CY DNILSDP PRPLF EYLEM+IQFGFITIFI FPLAPLLA+ NNL EIRSDA KF RRPVAQRVKDIGIW+ I++ +A I VKSNA+LIAFAT+YID++IYII +S N L GF DFSLS+MN++EFN + + +NET+C Y GFR+PPW+ EP SYTPV+WQ+LAAK+ FVYLFE V LF+ I ++IP++PD+ RIAMKREA +TNE ILK +L + +N + + +A S KK+ +
Sbjct: 18 NEKEKNNKGMFFTDRVRRIDFVLVYQQLLDSEEKRNKRISFIESLIDEGLEIEIQNIGGRIINIADSVNENSDDITCES--KNLVFVKIHASWDTLCRYAEIMKIRKSIMNAGVDYNY----IFQSFFEKFVDFDFFRIFSIDNKRLKPIAQGYTYPFSKQKYYLFDIPVNRSEFFTPVERSLIVNYILKRKGY-----KSNHKPIS-----NLSNEKMLVELEPIHSDKRSNSNDSITNEVEENGS-----------NLNFGIAKLISEKVFETAFILHEK----FSNGKYKKENMRVLLYKHWATLTNIFRHQPLDHIRLYFGEMVAFYFAWLGFYTFWLFPIALFGVCVTIYNLATIPRDIIIQEISERNNITMCPVFCIEGSCESWKLSAGYVQFYLIRLIDNGATVILAGVVSVWVFLFLEMWERKQFTLAFRWNVHDLEVEDEPLRPEYAALLEKNYISQFNKISGKEEPFISFWKNTLPIQMLTISTILFSIVLTLACLTGITVTKTIIRNLLRLQLTYVMSKLS---TAAIGSLISLITIIILTSVYSRVAVWLTDLEHHRTQSQYDNSVTGKLYILEFINNYSSMFYIGFAHPYTKVKPGSDSFSMIKPQGCLTGDCLLDLSMQLIFILVGKQIISNIINFSIPRLCSSIKEYRIKRADSKFIKRRSVH------RPTITD-YEISEVECSDIYTDCNGVKLQSELKICYQDNILSDPEPRPLFKEYLEMIIQFGFITIFILPFPLAPLLAIINNLMEIRSDAKKFVTVYRRPVAQRVKDIGIWMDILTTIAKIVVKSNALLIAFATSYIDRMIYIIVHSRNFDLGGFVDFSLSFMNTTEFNIS-------SSISNETHCSYIGFRYPPWDVHNEPMSYTPVFWQMLAAKIAFVYLFETFVSLFVKIFLMIIPNIPDEYRIAMKREAAITNEFILKIELEEHRNIYEANNLAKSLKKTII 938
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000033730.1 (SMESG000033730.1) HSP 1 Score: 857.055 bits (2213), Expect = 0.000e+0 Identity = 466/931 (50.05%), Postives = 631/931 (67.78%), Query Frame = 1
Query: 160 NNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMN--FDTNCQAPSGDKPFA-LEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSMIKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILK 2943
NNG+FF DG+R+IDFVL Y+++NDN N+ R FLK L+K+GLE EIQD+ ++ + +DN IVFIK+FA WETLC YAE+L+IRK +L + + F++ F FD N++ N FT P+S SKEYLFDIP + FF ERSLIVNYILR + + +KC I +I + S D+EM T + N+ +E + N + G L ++K + + LISE VF+SAY LHE + + ++R LYK W RNI R QPLD I+ YFGEK+AFYF+WLG+YTYWL SLLG++++++NI++ S+DI+V EI +G NITMCPL+C+S+ C+FWKLSD Y +FF+IR+LDN VLMA MSIWASLFLEMWKR+Q +LA+HWNV+D++ + EP +PEFVA I +N K N E +PFWRR +P MFF +STVL S+ +ALAFL GIILY++V ++L+ MH S ++ MS++I + TGS++SL++I+ LT+VY+++A WLT +E++ TQS+YD SVT+KLYLLEFINNYS +FY GFI P QPIP F++ S +KP GCP GGCL LS+QLAFIMIG+Q+ISH EY+ P L S++K+YKL++ ++V+ K+ V E+IRCY D +L+D +P+F EYLEMLIQFGFIT+FIPAFPLAPLLAL NNL EIR+DA KF A +RPVAQRVKDIG W++I+S+VA +AVKSNAILIAF T +I++L+YI+ YS G L GF+DF+LSY+ SSEF ++ TNETYCRY FR+ P + K P + T +YW+ILAAKV+FVYLFEN+VY M II+ IP+ +++ + +E T++ +++LK
Sbjct: 20 NNGLFFTDGIRQIDFVLAYQKLNDNERNRKIRSKFLKILMKQGLEFEIQDEEGNLFSLEN---FDNQD-----DTKKIVFIKLFAKWETLCHYAELLRIRKPILHPPSSIKYEQSLSFRKYFRKFDIFNLYFGNSFHSRTHTFTMPFSSSKEYLFDIPANRQYFFIQTERSLIVNYILRSITLQCNRNKCNI-LANILHPKSTPSSITDMEMRTFSTINSESECSKSHSNNDETDEGLTEIQHLIQTNDKLTNMSLHNLISEQVFESAYCLHE--SFIVQNSLKRDESDRVNLYKCWGLTRNICRSQPLDSIRSYFGEKIAFYFSWLGFYTYWLFFPSLLGLVITVFNILHASSDIIVNEIKEGGNITMCPLLCNSKQCKFWKLSDVYYKFFVIRILDNKTMVLMAFSMSIWASLFLEMWKREQFRLAFHWNVYDLQNQYEPQKPEFVAFITQNQTDKGN------ESSVPFWRRTVPFMFFKFSTVLFSVILALAFLVGIILYEIVIYALLMMHPSPLVTQMSNVIISVTGSIISLIVILLLTMVYAKLAHWLTGLENNETQSEYDDSVTMKLYLLEFINNYSYLFYFGFILPLIQPIPGFKAMSDLKPIGCPTGGCLFGLSVQLAFIMIGRQLISHFTEYSYPLLCSSIKKYKLRK--------------CNKVKYRSKNITN-----------VNAEIIRCYDDLLLADLESQPVFYEYLEMLIQFGFITMFIPAFPLAPLLALFNNLLEIRNDATKFLTAYKRPVAQRVKDIGTWINILSVVAGLAVKSNAILIAFTTFFIERLLYILKYSPTGDLKGFSDFTLSYLKSSEFASS----------TNETYCRYMDFRYHPLDIKNGPLTLTSIYWEILAAKVIFVYLFENVVYTIMKIISATIPNQNERITKEITKEVTISRKLLLK 898
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000033730.1 (SMESG000033730.1) HSP 1 Score: 530.406 bits (1365), Expect = 2.655e-175 Identity = 285/584 (48.80%), Postives = 391/584 (66.95%), Query Frame = 1
Query: 160 NNGMFFVDGVRKIDFVLVYERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSLGFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMN--FDTNCQAPSGDKPFA-LEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTNITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYS 1902
NNG+FF DG+R+IDFVL Y+++NDN N+ R FLK L+K+GLE EIQD+ ++ + +DN IVFIK+FA WETLC YAE+L+IRK +L + + F++ F FD N++ N FT P+S SKEYLFDIP + FF ERSLIVNYILR + + +KC I +I + S D+EM T + N+ +E + N + G L ++K + + LISE VF+SAY LHE + + ++R LYK W RNI R QPLD I+ YFGEK+AFYF+WLG+YTYWL SLLG++++++NI++ S+DI+V EI +G NITMCPL+C+S+ C+FWKLSD Y +FF+IR+LDN VLMA MSIWASLFLEMWKR+Q +LA+HWNV+D++ + EP +PEFVA I +N K N E +PFWRR +P MFF +STVL S+ +ALAFL GIILY++V ++L+ MH S ++ MS++I + TGS++SL++I+ LT+VY+++A WLT +E++ TQS+YD SVT+KLYLLEFINNYS
Sbjct: 20 NNGLFFTDGIRQIDFVLAYQKLNDNERNRKIRSKFLKILMKQGLEFEIQDEEGNLFSLEN---FDNQD-----DTKKIVFIKLFAKWETLCHYAELLRIRKPILHPPSSIKYEQSLSFRKYFRKFDIFNLYFGNSFHSRTHTFTMPFSSSKEYLFDIPANRQYFFIQTERSLIVNYILRSITLQCNRNKCNI-LANILHPKSTPSSITDMEMRTFSTINSESECSKSHSNNDETDEGLTEIQHLIQTNDKLTNMSLHNLISEQVFESAYCLHE--SFIVQNSLKRDESDRVNLYKCWGLTRNICRSQPLDSIRSYFGEKIAFYFSWLGFYTYWLFFPSLLGLVITVFNILHASSDIIVNEIKEGGNITMCPLLCNSKQCKFWKLSDVYYKFFVIRILDNKTMVLMAFSMSIWASLFLEMWKREQFRLAFHWNVYDLQNQYEPQKPEFVAFITQNQTDKGN------ESSVPFWRRTVPFMFFKFSTVLFSVILALAFLVGIILYEIVIYALLMMHPSPLVTQMSNVIISVTGSIISLIVILLLTMVYAKLAHWLTGLENNETQSEYDDSVTMKLYLLEFINNYS 586
BLAST of Anoctamin vs. Planmine SMEST
Match: SMESG000006381.1 (SMESG000006381.1) HSP 1 Score: 385.185 bits (988), Expect = 1.253e-117 Identity = 297/944 (31.46%), Postives = 460/944 (48.73%), Query Frame = 1
Query: 163 NGMFFVDGVRKIDFVLVY-ERINDNRTNQIKRMTFLKSLLKEGLEIEIQDDGCKVYPFKENSYYDNDSVGSPSHRSNIVFIKVFATWETLCRYAEVLKIRKQMLSL-GFEYDFNRKTIFQRLFETFDFLNIFNINELKPIPRGFTTPYSKSKEYLFDIPEDNATFFTAAERSLIVNYILRRASYRRISDKCAIEPVDIDVVEDILSRRCDVEMATITDENNVNEMNFDTNCQAPSGDKPFALEDISNKTVDFGIAKLISEGVFDSAYILHERPXXXXXXXXXXXXXXXXXXYKHWVSMRNIFRLQPLDHIKLYFGEKVAFYFAWLGYYTYWLLPISLLGIIVSLYNIVYTSNDIVVKEIYQGTN-ITMCPLMCSSRGCRFWKLSDAYSRFFLIRLLDNGATVLMAVVMSIWASLFLEMWKRQQVQLAYHWNVHDMEYEDEPARPEFVALIDKNYPKKTNLISGKEEPFIPFWRRRMPVMFFTYSTVLLSIAMALAFLGGIILYKLVSFSLMRMHHSRTISNMSSIITAATGSLLSLVIIMSLTLVYSRVAVWLTDMEHHRTQSQYDSSVTLKLYLLEFINNYSSIFYIGFIQPFTQPIPSFRSSSM-IKPRGCPAGGCLLDLSLQLAFIMIGKQIISHIKEYALPRLVSTVKRYKLKQAEEKLINQSDAEMEADRVEVAIKDKATILDPKCIDTWKVEPELIRCYADNILSDPGPRPLFGEYLEMLIQFGFITXXXXXXXXXXXXXXTNNLFEIRSDALKFARALRRPVAQRVKDIGIWVSIMSMVATIAVKSNAILIAFATTYIDKLIYIIGYSENGFLNGFTDFSLSYMNSSEFNATLYGGHKLTLGTNETYCRYSGFRHPPWETKYEPYSYTPVYWQILAAKVVFVYLFENLVYLFMAIIAVVIPDVPDKLRIAMKREATLTNEIILKADLLQMKNQHTSK 2982
+G +F DG KIDF+L + E+ + + + R F +L K GLE+E D D G +IK++ WE +CR+AE L +R + + D++ K + NI ++ P +T + +SK + F + T+F++ +R +VN IL Y R R+ ++ + + D+ + P + P+ +KT +L +S IL+E +W ++ QPLDHI+ YFGEK+ FYFAW+G YT WLLP S++GI V LY + +I KEI G N MCPL GC++W+L D + L D+ T+ ++ M++WA FLE WKR+ LA+HW+V D E E+E R ++ AL + N ++G EP+ P RR+P + +LL + + + F+ +I+Y+++ + M + + N + T ++++LV+IM L VY +A+ LT+ E HRTQS +D+ + K++L +F+N YSSIFYI F + P S ++ C GGCL++L++QLA IM+GKQIIS+I+E +P+L + R+ E+ Q D+ C ++ LI DN L L EYLE+++QFGFITIF+ AFPLAP AL NN EIR DA K +RPVA+R KDIG+W I+ + TIA SNA L+AF + ++ KL Y + L+G+T+ SLS+ S N+T+ CRY G+R K +W++LA ++ FV +FE+ V ++ ++IPDVP +L I +KRE L + + ADL+ + K
Sbjct: 36 SGYYFNDGKSKIDFILAWSEKEKEPHSKALYRKKFESNLKKAGLELE----------------EDFDETGK------FHYIKIYVPWEIMCRFAEKLALRAPLQAHPNPSDDWSMKAL-----NCLHIPNIMYLDAPNKPPDFYTCEFKQSKIHRFLGSNNKLTYFSSTQRHQVVNEILETQLYGR-------------------RRKGEIGIKRMVDDEIYDSA-------YPLHEGPYE----CSKT------ELEQPEKLNSRQILYE----------------------YWARYGRWYQYQPLDHIREYFGEKIGFYFAWMGLYTAWLLPASIIGIFVFLYGVFTFDRNISAKEICAGNNSFYMCPLCDEKIGCQYWELKDVCMFIKIAYLFDHPGTIFYSIFMAMWAVTFLEYWKRKNASLAHHWDVMDYEEEEERPRAQYAALCSIF---QKNPVTGVIEPYFPK-ERRLPRILTGIVCILLMMTVVIIFIIAVIIYRVL--IEIPMFQNPLLRNNAVTFANGTAAIVNLVLIMCLAKVYEIIALKLTEWEMHRTQSDFDNQLIFKVFLFQFVNFYSSIFYIAFFKGRFVGYPGNYSYFFGLRNEDCQNGGCLIELAMQLAIIMVGKQIISNIQEVIVPKLQTWFYRFSKGLGEQV---QHDS---------------------CSNS-----GLIIYKEDNKLIP--YEGLLDEYLEIVLQFGFITIFVAAFPLAPFFALLNNWMEIRLDAQKLVCETQRPVAERAKDIGVWFVILESITTIACISNAFLLAFTSEFLPKLYYRYRFDS---LDGYTNHSLSW---SPKNSTVIP------------CRYPGYRDNDGILK-------QYFWELLAVRLGFVIVFEHTVAGIGKLLDLLIPDVPKELYIKIKRETYLAKQALQDADLITWETFENEK 832 The following BLAST results are available for this feature:
BLAST of Anoctamin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 4
BLAST of Anoctamin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Anoctamin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Anoctamin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Anoctamin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Anoctamin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Anoctamin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Anoctamin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Anoctamin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016964 ID=SMED30016964|Name=Anoctamin|organism=Schmidtea mediterranea sexual|type=transcript|length=3498bpback to top protein sequence of SMED30016964-orf-1 >SMED30016964-orf-1 ID=SMED30016964-orf-1|Name=SMED30016964-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1020bp MSNEDEILAADNPDINDDKKYNNGMFFVDGVRKIDFVLVYERINDNRTNQback to top Annotated Terms
The following terms have been associated with this transcript:
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