Regulator of G-protein signaling 9-binding protein

Overview
NameRegulator of G-protein signaling 9-binding protein
Smed IDSMED30016948
Length (bp)1502
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Regulator of G-protein signaling 9-binding protein (SMED30016948) t-SNE clustered cells

Violin plots show distribution of expression levels for Regulator of G-protein signaling 9-binding protein (SMED30016948) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Regulator of G-protein signaling 9-binding protein (SMED30016948) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Regulator of G-protein signaling 9-binding protein (SMED30016948) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016948

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30016948SMESG000016932.1 SmedASXL_001208SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30016948SMESG000016932.1 dd_Smed_v4_14671_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016948SMESG000016932.1 dd_Smed_v4_14671_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30016948SMESG000016932.1 dd_Smed_v4_14671_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
anterior sideSMED30016948SMESG000016932.1 dd_Smed_v4_14671_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016948SMESG000016932.1 dd_Smed_v4_14671_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30016948SMESG000016932.1 dd_Smed_v4_14671_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016948SMESG000016932.1 Contig31995newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016948SMESG000016932.1 Contig31995uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30016948SMESG000016932.1 dd_Smed_v6_14671_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Human
Match: RGS7BP (regulator of G protein signaling 7 binding protein [Source:HGNC Symbol;Acc:HGNC:23271])

HSP 1 Score: 50.447 bits (119), Expect = 3.065e-6
Identity = 30/104 (28.85%), Postives = 52/104 (50.00%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMR-DTGQLREDIHAIRQRGWELAN--HNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLN 828
            V+EF+   ALY  L++S+G +      LR ++H  R +G E+A   H KL  +         G  D   +  + R++     CLE +  ++L+++ LL S Q +
Sbjct:   57 VQEFNTQVALYRELVISIGDVSVSCPSLRAEMHKTRTKGCEMARQAHQKLAAI--------SGPEDGEIHPEICRLYIQLQCCLEMYTTEMLKSICLLGSLQFH 152
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Zebrafish
Match: rgs7bpa (regulator of G protein signaling 7 binding protein a [Source:NCBI gene;Acc:767669])

HSP 1 Score: 54.6842 bits (130), Expect = 6.419e-8
Identity = 33/104 (31.73%), Postives = 54/104 (51.92%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMR-DTGQLREDIHAIRQRGWELAN--HNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLN 828
            V+EF+   ALY  L++S+G +  D   LR ++H  R +G E+A   H  L  +         G  D   +  + R+F     CLE ++ ++L++V LL S QL+
Sbjct:   49 VQEFNTLVALYRELVISIGEISADCPSLRAEMHKTRTKGCEMARTAHQNLSAI--------SGPEDGEIHPEICRLFIQLQCCLEMYLTEMLKSVCLLGSLQLH 144
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Xenopus
Match: fgfr1op (FGFR1 oncogene partner [Source:Xenbase;Acc:XB-GENE-5752647])

HSP 1 Score: 48.521 bits (114), Expect = 7.762e-6
Identity = 29/110 (26.36%), Postives = 53/110 (48.18%), Query Frame = 1 
Query:  499 LITPEVERNVREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLN 828
            ++  E +  +   ++ TA Y  L+L++G   D+  LRE++   RQ+  ELA  N+  L   ++    D  L        ER++ +F +C++     + R + L   F LN
Sbjct:    1 MLKEECKALLDALNKVTACYRHLVLTIGGTSDSQNLREELKKTRQKAQELAVANRNKLTSTLK----DHRLTKEDKAEFERLWVIFTTCMDILETDMRRALVLGQEFPLN 106
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Mouse
Match: Rgs7bp (regulator of G-protein signalling 7 binding protein [Source:MGI Symbol;Acc:MGI:106334])

HSP 1 Score: 50.0618 bits (118), Expect = 2.571e-6
Identity = 30/104 (28.85%), Postives = 52/104 (50.00%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMR-DTGQLREDIHAIRQRGWELAN--HNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLN 828
            V+EF+   ALY  L++S+G +      LR ++H  R +G E+A   H KL  +         G  D   +  + R++     CLE +  ++L+++ LL S Q +
Sbjct:   57 VQEFNTQVALYRELVISIGDVSVSCPSLRAEMHKTRTKGCEMARQAHQKLAAI--------SGPEDGEIHPEICRLYIQLQCCLEMYTTEMLKSICLLGSLQFH 152
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Mouse
Match: Rgs9bp (regulator of G-protein signalling 9 binding protein [Source:MGI Symbol;Acc:MGI:2384418])

HSP 1 Score: 49.6766 bits (117), Expect = 2.871e-6
Identity = 37/125 (29.60%), Postives = 57/125 (45.60%), Query Frame = 1 
Query:  535 FSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNG-HPDCGTFVNSGLSDQMPGSLYLRA 906
             ++ TA Y  L+L+VG   DT  LRE++   RQ+  ELA         R+  A  D +L +      ER++  F  CL+     + R + L ++F L+    P   T V  G S     +L  R+
Sbjct:   13 LNKTTACYHHLVLTVGGSADTQDLREELQKTRQKARELAVATG----ARLTVALRDRSLATEERAEFERLWVAFSGCLDLLEADMQRALALGATFPLHAPRRPLVRTGVTGGSSAVAARALSARS 133
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. UniProt/SwissProt
Match: sp|Q6XK22|R9BP_CHICK (Regulator of G-protein signaling 9-binding protein OS=Gallus gallus OX=9031 GN=RGS9BP PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.068e-7
Identity = 35/112 (31.25%), Postives = 55/112 (49.11%), Query Frame = 1 
Query:  499 LITPEVERNVREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWEL--ANHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLN 828
            ++  E +  +   SR TA Y  ++L++G   D+  LRE++   RQ+  EL  AN NKL  V R      D  +        ER++ +F +CLE     + R ++L   F LN
Sbjct:    1 MVKEECKALLDALSRVTACYRHMVLTIGGTSDSQNLREELKKTRQKAQELAVANRNKLTTVLR------DKTVSKEDKAEFERLWVIFSTCLEILEIDMRRALELGHEFPLN 106
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. UniProt/SwissProt
Match: sp|Q08BU8|R7BPA_DANRE (Regulator of G-protein signaling 7-binding protein A OS=Danio rerio OX=7955 GN=rgs7bpa PE=2 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 4.845e-7
Identity = 33/104 (31.73%), Postives = 54/104 (51.92%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMR-DTGQLREDIHAIRQRGWELAN--HNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLN 828
            V+EF+   ALY  L++S+G +  D   LR ++H  R +G E+A   H  L  +         G  D   +  + R+F     CLE ++ ++L++V LL S QL+
Sbjct:   49 VQEFNTLVALYRELVISIGEISADCPSLRAEMHKTRTKGCEMARTAHQNLSAI--------SGPEDGEIHPEICRLFIQLQCCLEMYLTEMLKSVCLLGSLQLH 144
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. UniProt/SwissProt
Match: sp|Q08DH5|R7BP_BOVIN (Regulator of G-protein signaling 7-binding protein OS=Bos taurus OX=9913 GN=RGS7BP PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 2.503e-6
Identity = 31/104 (29.81%), Postives = 53/104 (50.96%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMR-DTGQLREDIHAIRQRGWELAN--HNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLN 828
            V+EF+   ALY  L++S+G +      LRE++H  R +G E+A   H KL  +         G  D   +  + R++     CLE +  ++L+++ LL S Q +
Sbjct:   57 VQEFNTQVALYRELVISIGDVSVSCPSLREEMHKTRTKGCEMARQAHQKLAAI--------SGPEDGEIHPEICRLYIQLQCCLEMYTTEMLKSICLLGSLQFH 152
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. UniProt/SwissProt
Match: sp|Q6GLU0|R9BPC_XENLA (Regulator of G-protein signaling 9-binding protein C OS=Xenopus laevis OX=8355 GN=rgs9bp-c PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 4.159e-6
Identity = 31/100 (31.00%), Postives = 48/100 (48.00%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELA--NHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSF 819
            V    +  A Y  L+L++G   D+  LR+++   R+R  ELA  N NKL    R      D  L     + LER++  F SCLE F   + +  +L ++ 
Sbjct:   27 VESLHKVVACYRHLVLTIGGSSDSIHLRDELRRTRERAQELAVCNRNKLTTALR------DKKLSKKDCEELERLWVEFSSCLELFHNDMCKVYELATAL 120
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. TrEMBL
Match: A0A210QFL3 (Regulator of G-protein signaling 9-binding protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT15598 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.041e-11
Identity = 43/125 (34.40%), Postives = 71/125 (56.80%), Query Frame = 1 
Query:  502 ITPEVERNVREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGHPDCGTFVNSGLSD 876
            I  E  + V E ++ TA Y    + +G  +D+  LRE++  IR RG ELA  N+L LVP +  A     L +   + LER++ +F +CLE    QL++T+ L+ +F L+NG       +N+G+++
Sbjct:   38 IIQECAKIVVELNQETAAYCTHAVYIGGTQDSEDLREELKRIRLRGLELAQLNRLKLVPLLSNA----TLGTDDAQELERLYKIFSACLELLEIQLIKTLALVQAFPLHNGTK---VLINTGITE 155
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. TrEMBL
Match: A0A2T7NE07 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_19887 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.298e-9
Identity = 40/124 (32.26%), Postives = 70/124 (56.45%), Query Frame = 1 
Query:  535 FSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGHPDCGTFVNSGLSDQMPGSLYLRA 906
             S+  AL+  L + +G + D+ +LR+++   R R   LA+ NK++L+P ++      +        LER++ ++ +CLEFF  QLL+   LL+ F L   H +   F+N+G++D  P S+  RA
Sbjct:   51 LSKEIALFCNLSMGLGGVSDSDRLRDELRQSRDRACALAHENKIILLPLLKSTRKSSD----TRGELERLYRLYSACLEFFEIQLLKVRSLLALFPL---HTETCLFINTGIAD--PLSILSRA 165
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. TrEMBL
Match: A0A0R3TGA4 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS6093 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.687e-7
Identity = 45/125 (36.00%), Postives = 63/125 (50.40%), Query Frame = 1 
Query:  535 FSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVP--------RMRQANNDGNLDSPAYK------NLE---RVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGHPDCGTFV 858
            FS   ALY+ LI  +GT  D  +LR     +R+R    A   + +L+P         +R  +   N+ S +Y+      NLE    +FF+ C+CLEFF+ QLLRT  L   F LN G P   +FV
Sbjct:   63 FSHYIALYSTLISRLGTRCDCRKLRAKSEEVRRRNMYYARKCQEVLLPIVESVKVSLLRPPSASRNIYS-SYRRGSRKSNLEDYYHLFFLHCTCLEFFISQLLRTAQLFQCFSLNIGSPLTSSFV 186
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. TrEMBL
Match: A0A1S3J9J2 (regulator of G-protein signaling 9-binding protein OS=Lingula unguis OX=7574 GN=LOC106170991 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 2.033e-6
Identity = 36/107 (33.64%), Postives = 62/107 (57.94%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNLDSPAYKNL---ERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGH 837
            ++E S+ TA Y ML++++GT  D   LR+++   + R ++L   NK  L+P +R  NN     + + ++L   ER++ +F SCLE     L +TV L  +F L+ G+
Sbjct:   36 LKELSKYTAHYEMLMIALGTTCDNDSLRKELRRTQGRAYDLVLQNKARLMPYLR--NNSAACTTISKEDLQEFERLWSIFMSCLESLESHLCKTVMLQKAFPLHQGN 140
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. TrEMBL
Match: R7VFR3 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_204254 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 3.642e-6
Identity = 35/105 (33.33%), Postives = 60/105 (57.14%), Query Frame = 1 
Query:  520 RNVREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNL-DSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNN 831
            +++++F+R TA Y  LI ++G+  D+  LR ++ +  ++  ELAN NK  L+P ++     G L      +  E+++  F SCLE    Q+ RTV LL+ F L+ 
Sbjct:   57 KSMKDFNRETARYCCLIPAIGSTVDSISLRNELKSTWKKAHELANQNKSRLLPHLK-----GRLCKIDEVEEYEKIWTTFGSCLENLQSQMWRTVSLLACFPLHG 156
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Cavefish
Match: rgs7bpa (regulator of G protein signaling 7 binding protein [Source:NCBI gene;Acc:103040426])

HSP 1 Score: 54.6842 bits (130), Expect = 4.178e-8
Identity = 36/116 (31.03%), Postives = 57/116 (49.14%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMR-DTGQLREDIHAIRQRGWELAN--HNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGHPDCGTFVNS 864
            V+EF+   ALY  L++SVG +  D   LR ++   R +G E+A   H KL  +         G  D   +  + R+F     CLE ++ ++L++V LL S QL+    D    + S
Sbjct:   34 VQEFNTLVALYRELVISVGEISVDCPSLRAEMQKTRTKGCEMARTAHQKLSAI--------SGPEDGEIHPEICRLFIQLQCCLEMYLTEMLKSVCLLESLQLHRRGKDSALKLES 141
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Medaka
Match: rgs7bpa (regulator of G protein signaling 7 binding protein [Source:NCBI gene;Acc:101164065])

HSP 1 Score: 54.299 bits (129), Expect = 6.686e-8
Identity = 35/112 (31.25%), Postives = 58/112 (51.79%), Query Frame = 1 
Query:  526 VREFSRCTALYTMLILSVGTMR-DTGQLREDIHAIRQRGWELAN--HNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLN-NGHPDCG 849
            V+EF+   ALY  L++S+G +  D   LR ++   R +G E+A   H+ L L+         G  D   +  + R+F     CLE ++ ++L++V +L S QL+  G   CG
Sbjct:   52 VQEFNTLVALYRELVISIGEITVDCPSLRAEMLKTRTKGCEMARAAHHSLSLI--------SGPEDGEIHPEICRLFIQLQCCLEMYITEMLKSVCMLGSLQLHMKGKDPCG 155
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Medaka
Match: rgs7bpb (regulator of G protein signaling 7 binding protein b [Source:NCBI gene;Acc:337556])

HSP 1 Score: 48.521 bits (114), Expect = 6.351e-6
Identity = 34/116 (29.31%), Postives = 54/116 (46.55%), Query Frame = 1 
Query:  523 NVREFSRCTALYTMLILSVGTMR-DTGQLREDIHAIRQRGWELAN--HNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGHPDCGTFVN 861
             V+EF+   ALY   ++SVG M  D   LR  +H  R +G  +A   H  L ++      +  G  D      + R+F     CLE F+ ++L+++ LL   QL+    D    V+
Sbjct:   42 TVQEFNTLVALYREQVISVGEMSADCPPLRAQMHHTRTKGCSMARAAHQDLAVI------SVSGPEDGEIPPEICRLFIQLQCCLEMFVTEMLKSMCLLGVLQLHRRRTDSDPKVD 151
[Close]
BLAST of Regulator of G-protein signaling 9-binding protein vs. Planmine SMEST
Match: SMESG000016932.1 (SMESG000016932.1)

HSP 1 Score: 622.854 bits (1605), Expect = 0.000e+0
Identity = 342/343 (99.71%), Postives = 342/343 (99.71%), Query Frame = 1 
Query:  361 MSNRSTNEQKTCETKPDKVKQKPPTGKTKTSVAINVDVISLKQHQSLITPEVERNVREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGHPDCGTFVNSGLSDQMPGSLYLRAAGXXXXXXXXXXXXXXTARRHSAFNFRFNTPRLKQHQKQNALLGDKNAKIENQELQXXXXXXXXXXXXXEIHSSIAMTPWTIQPQYHLDRFAGNXXXXXXXXXXXXEGGLDSVRSSTCKFSQGMENTDQDSRINTKQRRCLILTIIALVSVIFIAIALGVG 1389
            MSNRSTNEQK CETKPDKVKQKPPTGKTKTSVAINVDVISLKQHQSLITPEVERNVREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGHPDCGTFVNSGLSDQMPGSLYLRAAGSSISASPLSSPIMSTARRHSAFNFRFNTPRLKQHQKQNALLGDKNAKIENQELQLKKDIKALKDLILEIHSSIAMTPWTIQPQYHLDRFAGNSDSLASLADSSDEGGLDSVRSSTCKFSQGMENTDQDSRINTKQRRCLILTIIALVSVIFIAIALGVG
Sbjct:    1 MSNRSTNEQKICETKPDKVKQKPPTGKTKTSVAINVDVISLKQHQSLITPEVERNVREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHNKLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLLSSFQLNNGHPDCGTFVNSGLSDQMPGSLYLRAAGSSISASPLSSPIMSTARRHSAFNFRFNTPRLKQHQKQNALLGDKNAKIENQELQLKKDIKALKDLILEIHSSIAMTPWTIQPQYHLDRFAGNSDSLASLADSSDEGGLDSVRSSTCKFSQGMENTDQDSRINTKQRRCLILTIIALVSVIFIAIALGVG 343
The following BLAST results are available for this feature:
BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RGS7BP3.065e-628.85regulator of G protein signaling 7 binding protein... [more]
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rgs7bpa6.419e-831.73regulator of G protein signaling 7 binding protein... [more]
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fgfr1op7.762e-626.36FGFR1 oncogene partner [Source:Xenbase;Acc:XB-GENE... [more]
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rgs7bp2.571e-628.85regulator of G-protein signalling 7 binding protei... [more]
Rgs9bp2.871e-629.60regulator of G-protein signalling 9 binding protei... [more]
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BLAST of Regulator of G-protein signaling 9-binding protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q6XK22|R9BP_CHICK1.068e-731.25Regulator of G-protein signaling 9-binding protein... [more]
sp|Q08BU8|R7BPA_DANRE4.845e-731.73Regulator of G-protein signaling 7-binding protein... [more]
sp|Q08DH5|R7BP_BOVIN2.503e-629.81Regulator of G-protein signaling 7-binding protein... [more]
sp|Q6GLU0|R9BPC_XENLA4.159e-631.00Regulator of G-protein signaling 9-binding protein... [more]
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BLAST of Regulator of G-protein signaling 9-binding protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210QFL37.041e-1134.40Regulator of G-protein signaling 9-binding protein... [more]
A0A2T7NE071.298e-932.26Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A0R3TGA41.687e-736.00Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
A0A1S3J9J22.033e-633.64regulator of G-protein signaling 9-binding protein... [more]
R7VFR33.642e-633.33Uncharacterized protein OS=Capitella teleta OX=283... [more]
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rgs7bpa4.178e-831.03regulator of G protein signaling 7 binding protein... [more]
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rgs7bpa6.686e-831.25regulator of G protein signaling 7 binding protein... [more]
rgs7bpb6.351e-629.31regulator of G protein signaling 7 binding protein... [more]
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BLAST of Regulator of G-protein signaling 9-binding protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000016932.10.000e+099.71SMESG000016932.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016948 ID=SMED30016948|Name=Regulator of G-protein signaling 9-binding protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1502bp
GGAGAGGTAATATCTAGTGACAGACATTGGCTGTTTTTTTAAAGGATTAC
AACACACTTATTTGATCTTGATGTTATGTTCTGTTGGGAGAAATACAACT
GTCCCTGGTTATCGAGTTCGCTTGTATAGATAGTAAGAAAATACATTCCC
CGGTCATTGACATCCCTTCTCTCTTGAAAAAGGGGGAAGAAATATTTTCT
AAAAGTAAATTTAGATTTAGATAGACAAAATCTCCCAACAACAATCAATA
AATATTATCTTTACTCATAAACAAGAAGCCCAACCGTTCAAGAATTCATC
GAAAGTATTCAAAGAAAAAAAAAGAAAAAAAAAAGAAAAAAAAGTTTTTT
TCCCCCTCAAATGTCTAATCGTTCGACAAATGAACAAAAAACCTGCGAAA
CAAAACCCGACAAAGTCAAACAGAAACCACCGACCGGTAAAACTAAAACC
AGTGTCGCGATTAATGTCGATGTGATTTCACTTAAACAACATCAGTCTTT
GATTACTCCTGAAGTGGAACGAAACGTTCGAGAGTTTTCTAGATGTACCG
CTCTCTATACCATGCTGATTCTCAGTGTTGGAACTATGAGGGATACCGGT
CAACTTCGAGAGGATATACACGCTATCAGACAACGAGGATGGGAACTTGC
CAACCATAATAAGTTGTTACTCGTTCCGAGAATGCGACAAGCCAACAATG
ATGGGAATTTAGATTCACCGGCATACAAAAATTTGGAACGCGTATTTTTC
ATGTTTTGTTCTTGTCTTGAATTTTTTATGGAGCAACTGTTACGAACCGT
TGACTTGTTGAGTAGTTTTCAGTTGAATAATGGACACCCAGATTGTGGAA
CATTTGTCAATTCTGGTTTGAGTGATCAAATGCCCGGTTCGTTATATCTA
AGAGCTGCTGGTTCATCTATTAGTGCTTCTCCATTATCATCTCCGATTAT
GAGTACTGCCAGGCGGCATTCGGCTTTCAATTTTCGTTTCAACACTCCTC
GATTAAAACAACATCAAAAACAAAACGCCTTACTTGGAGATAAGAATGCT
AAAATCGAAAATCAAGAACTCCAACTGAAAAAGGATATCAAAGCGTTAAA
GGATCTAATTTTAGAAATTCATTCATCAATAGCCATGACTCCTTGGACTA
TTCAGCCGCAATATCATTTGGATAGGTTTGCCGGCAACAGTGATTCCTTA
GCCTCTTTGGCTGACAGTTCAGACGAAGGCGGACTCGACAGTGTTCGATC
TTCGACTTGTAAATTCTCACAAGGTATGGAAAATACCGACCAGGACTCAA
GAATTAATACGAAACAACGGAGATGTTTAATATTAACAATTATTGCTCTC
GTATCAGTCATTTTTATTGCCATAGCATTGGGAGTTGGAATTAGATTACT
CAGATAGCCGTTGCGGATATTACATCATATACTGACCAATGGAGAACAGA
ATGTAACTGCTCAGACAATTTGTAAAGTTTTATTCCATTTTAAGGATAAG
AG
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protein sequence of SMED30016948-orf-1

>SMED30016948-orf-1 ID=SMED30016948-orf-1|Name=SMED30016948-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=349bp
MSNRSTNEQKTCETKPDKVKQKPPTGKTKTSVAINVDVISLKQHQSLITP
EVERNVREFSRCTALYTMLILSVGTMRDTGQLREDIHAIRQRGWELANHN
KLLLVPRMRQANNDGNLDSPAYKNLERVFFMFCSCLEFFMEQLLRTVDLL
SSFQLNNGHPDCGTFVNSGLSDQMPGSLYLRAAGSSISASPLSSPIMSTA
RRHSAFNFRFNTPRLKQHQKQNALLGDKNAKIENQELQLKKDIKALKDLI
LEIHSSIAMTPWTIQPQYHLDRFAGNSDSLASLADSSDEGGLDSVRSSTC
KFSQGMENTDQDSRINTKQRRCLILTIIALVSVIFIAIALGVGIRLLR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0000418head
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR026512RGS7BP/RGS9BP
Vocabulary: cellular component
TermDefinition
GO:0001750photoreceptor outer segment
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0009968negative regulation of signal transduction
GO:0050908detection of light stimulus involved in visual perception
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 215..252
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..24
NoneNo IPR availableTMHMMTMhelixcoord: 322..344
IPR026512RGS7BP/RGS9BP familyPANTHERPTHR21029FAMILY NOT NAMEDcoord: 51..338