Cytospin-A

Overview
NameCytospin-A
Smed IDSMED30016941
Length (bp)2284
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cytospin-A (SMED30016941) t-SNE clustered cells

Violin plots show distribution of expression levels for Cytospin-A (SMED30016941) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cytospin-A (SMED30016941) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cytospin-A (SMED30016941) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016941

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30016941SMESG000002849.1 dd_Smed_v4_14690_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30016941SMESG000002849.1 dd_Smed_v4_14690_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30016941SMESG000002849.1 dd_Smed_v6_14690_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cytospin-A vs. Ensembl Human
Match: SMTN (smoothelin [Source:HGNC Symbol;Acc:HGNC:11126])

HSP 1 Score: 91.2781 bits (225), Expect = 6.319e-25
Identity = 33/72 (45.83%), Postives = 52/72 (72.22%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFIF 1857
            ++K +LL W    T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY  LS  +  +NF +AF +A+ + 
Sbjct:  885 SIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEVAFSSAEMLV 956

HSP 2 Score: 44.2838 bits (103), Expect = 6.319e-25
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query: 1914 PLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSH 2012
            PL+E  D+ I+G  PD KCV+TY+Q +YN L+ H
Sbjct:  960 PLVEVDDMMIMGKKPDPKCVFTYVQSLYNHLRRH 993
[Close]
BLAST of Cytospin-A vs. Ensembl Human
Match: SMTN (smoothelin [Source:HGNC Symbol;Acc:HGNC:11126])

HSP 1 Score: 89.7373 bits (221), Expect = 4.060e-24
Identity = 33/69 (47.83%), Postives = 51/69 (73.91%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++K +LL W    T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY  LS  +  +NF +AF +A+
Sbjct:  800 SIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEVAFSSAE 868

HSP 2 Score: 43.1282 bits (100), Expect = 4.060e-24
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query: 1914 PLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSH 2012
            PL+E  D+ I+G  PD KCV+TY+Q +YN L+ H
Sbjct:  898 PLVEVDDMMIMGKKPDPKCVFTYVQSLYNHLRRH 931
[Close]
BLAST of Cytospin-A vs. Ensembl Human
Match: SMTNL2 (smoothelin like 2 [Source:HGNC Symbol;Acc:HGNC:24764])

HSP 1 Score: 85.8853 bits (211), Expect = 9.576e-22
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1 
Query: 1624 GKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            G  +  ++K +LL W    T  YQ+V + N+S SW+DG+AFCAL+H F+P++FDY+ LS     KNF LAF  A+
Sbjct:  346 GVASASSIKQILLEWCRSKTLGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNSLSPTQRQKNFELAFTMAE 420

HSP 2 Score: 38.891 bits (89), Expect = 9.576e-22
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1896 NYAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            N A  E LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  421 NLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 458
[Close]
BLAST of Cytospin-A vs. Ensembl Human
Match: SMTN (smoothelin [Source:HGNC Symbol;Acc:HGNC:11126])

HSP 1 Score: 89.7373 bits (221), Expect = 1.869e-21
Identity = 33/69 (47.83%), Postives = 51/69 (73.91%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++K +LL W    T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY  LS  +  +NF +AF +A+
Sbjct:  800 SIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEVAFSSAE 868

HSP 2 Score: 33.8834 bits (76), Expect = 1.869e-21
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSL 2003
            F + FS     S   +A    L++  D+  L  PD KCVYTYIQ  Y  L
Sbjct:  860 FEVAFS-----SAETHADCPQLLDTEDMVRLREPDWKCVYTYIQEFYRCL 904
[Close]
BLAST of Cytospin-A vs. Ensembl Human
Match: SMTNL2 (smoothelin like 2 [Source:HGNC Symbol;Acc:HGNC:24764])

HSP 1 Score: 85.1149 bits (209), Expect = 2.056e-21
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1 
Query: 1624 GKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            G  +  ++K +LL W    T  YQ+V + N+S SW+DG+AFCAL+H F+P++FDY+ LS     KNF LAF  A+
Sbjct:  202 GVASASSIKQILLEWCRSKTLGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNSLSPTQRQKNFELAFTMAE 276

HSP 2 Score: 38.5058 bits (88), Expect = 2.056e-21
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1896 NYAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            N A  E LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  277 NLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 314
[Close]
BLAST of Cytospin-A vs. Ensembl Celegans
Match: T15B12.1 (pep chromosome:WBcel235:III:5288180:5298361:1 gene:WBGene00020530.1 transcript:T15B12.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T15B12.1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.055e-23
Identity = 38/69 (55.07%), Postives = 48/69 (69.57%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++KD LL WI+     Y NV +TN+S SWADG+AFCALIH F P SFD+S L  N+   NF LAF+ A+
Sbjct:  324 SIKDALLRWIQNRVAGYPNVNVTNFSSSWADGMAFCALIHRFAPNSFDFSKLDPNNRRYNFDLAFKVAE 392

HSP 2 Score: 40.817 bits (94), Expect = 3.055e-23
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1905 GIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLKSHP 2015
            GI PL+E  D+ ++G  PD KCV+TY+Q  Y   + HP
Sbjct:  395 GIFPLLEVDDMIMMGDRPDWKCVFTYVQSFYKQFRDHP 432
[Close]
BLAST of Cytospin-A vs. Ensembl Celegans
Match: T15B12.1 (pep chromosome:WBcel235:III:5294501:5298361:1 gene:WBGene00020530.1 transcript:T15B12.1a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T15B12.1)

HSP 1 Score: 86.6557 bits (213), Expect = 5.654e-23
Identity = 38/69 (55.07%), Postives = 48/69 (69.57%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++KD LL WI+     Y NV +TN+S SWADG+AFCALIH F P SFD+S L  N+   NF LAF+ A+
Sbjct:  114 SIKDALLRWIQNRVAGYPNVNVTNFSSSWADGMAFCALIHRFAPNSFDFSKLDPNNRRYNFDLAFKVAE 182

HSP 2 Score: 41.2022 bits (95), Expect = 5.654e-23
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1905 GIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLKSHP 2015
            GI PL+E  D+ ++G  PD KCV+TY+Q  Y   + HP
Sbjct:  185 GIFPLLEVDDMIMMGDRPDWKCVFTYVQSFYKQFRDHP 222
[Close]
BLAST of Cytospin-A vs. Ensembl Celegans
Match: T15B12.1 (pep chromosome:WBcel235:III:5294620:5298361:1 gene:WBGene00020530.1 transcript:T15B12.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T15B12.1)

HSP 1 Score: 86.6557 bits (213), Expect = 5.654e-23
Identity = 38/69 (55.07%), Postives = 48/69 (69.57%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++KD LL WI+     Y NV +TN+S SWADG+AFCALIH F P SFD+S L  N+   NF LAF+ A+
Sbjct:  114 SIKDALLRWIQNRVAGYPNVNVTNFSSSWADGMAFCALIHRFAPNSFDFSKLDPNNRRYNFDLAFKVAE 182

HSP 2 Score: 41.2022 bits (95), Expect = 5.654e-23
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1905 GIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLKSHP 2015
            GI PL+E  D+ ++G  PD KCV+TY+Q  Y   + HP
Sbjct:  185 GIFPLLEVDDMIMMGDRPDWKCVFTYVQSFYKQFRDHP 222
[Close]
BLAST of Cytospin-A vs. Ensembl Celegans
Match: atn-1 (AcTiniN [Source:UniProtKB/TrEMBL;Acc:Q9XVU8])

HSP 1 Score: 83.9593 bits (206), Expect = 1.158e-16
Identity = 34/73 (46.58%), Postives = 47/73 (64.38%), Query Frame = 1 
Query: 1630 ITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            + +L+ +D LL+W ++ T  Y NV + N+  SW DGLAFCALIH   P+  DYS L   DP+ N  LAF+ A+
Sbjct:   76 VEELSARDGLLLWCQRKTAPYNNVNVQNFHNSWKDGLAFCALIHRHRPDLLDYSQLHKGDPIHNLNLAFDIAE 148

HSP 2 Score: 22.3274 bits (46), Expect = 1.158e-16
Identity = 7/24 (29.17%), Postives = 14/24 (58.33%), Query Frame = 3 
Query: 1938 NILGSPDEKCVYTYIQFMYNSLKS 2009
            ++   PDEK   TY+   Y++ ++
Sbjct:  161 DVSRHPDEKSTMTYVSCFYHAFRN 184
[Close]
BLAST of Cytospin-A vs. Ensembl Celegans
Match: atn-1 (AcTiniN [Source:UniProtKB/TrEMBL;Acc:Q9XVU8])

HSP 1 Score: 83.5741 bits (205), Expect = 1.430e-16
Identity = 34/73 (46.58%), Postives = 47/73 (64.38%), Query Frame = 1 
Query: 1630 ITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            + +L+ +D LL+W ++ T  Y NV + N+  SW DGLAFCALIH   P+  DYS L   DP+ N  LAF+ A+
Sbjct:  147 VEELSARDGLLLWCQRKTAPYNNVNVQNFHNSWKDGLAFCALIHRHRPDLLDYSQLHKGDPIHNLNLAFDIAE 219

HSP 2 Score: 22.3274 bits (46), Expect = 1.430e-16
Identity = 7/24 (29.17%), Postives = 14/24 (58.33%), Query Frame = 3 
Query: 1938 NILGSPDEKCVYTYIQFMYNSLKS 2009
            ++   PDEK   TY+   Y++ ++
Sbjct:  232 DVSRHPDEKSTMTYVSCFYHAFRN 255
[Close]
BLAST of Cytospin-A vs. Ensembl Fly
Match: CG34417 (gene:FBgn0085446 transcript:FBtr0336674)

HSP 1 Score: 100.138 bits (248), Expect = 5.517e-25
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1645 LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            +KD LL W +  TQEY+NV+I N+S SW+DGLAFCALIHHF P++FDY+ L+      NF LAF  A
Sbjct: 3391 VKDQLLQWCKHKTQEYENVQINNFSSSWSDGLAFCALIHHFLPDAFDYTTLTKQTRRHNFELAFSVA 3457

HSP 2 Score: 34.6538 bits (78), Expect = 5.517e-25
Identity = 14/36 (38.89%), Postives = 23/36 (63.89%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKS 2009
            AGI PL++  D+  +  PD KCV+ Y+Q +Y   ++
Sbjct: 3461 AGIAPLLDVEDMVEMSRPDWKCVFVYVQSIYRRFRN 3496
[Close]
BLAST of Cytospin-A vs. Ensembl Fly
Match: CG34417 (gene:FBgn0085446 transcript:FBtr0345070)

HSP 1 Score: 100.138 bits (248), Expect = 5.524e-25
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1645 LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            +KD LL W +  TQEY+NV+I N+S SW+DGLAFCALIHHF P++FDY+ L+      NF LAF  A
Sbjct: 3105 VKDQLLQWCKHKTQEYENVQINNFSSSWSDGLAFCALIHHFLPDAFDYTTLTKQTRRHNFELAFSVA 3171

HSP 2 Score: 34.6538 bits (78), Expect = 5.524e-25
Identity = 14/36 (38.89%), Postives = 23/36 (63.89%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKS 2009
            AGI PL++  D+  +  PD KCV+ Y+Q +Y   ++
Sbjct: 3175 AGIAPLLDVEDMVEMSRPDWKCVFVYVQSIYRRFRN 3210
[Close]
BLAST of Cytospin-A vs. Ensembl Fly
Match: CG34417 (gene:FBgn0085446 transcript:FBtr0336670)

HSP 1 Score: 98.5969 bits (244), Expect = 5.906e-25
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1645 LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            +KD LL W +  TQEY+NV+I N+S SW+DGLAFCALIHHF P++FDY+ L+      NF LAF  A
Sbjct: 1602 VKDQLLQWCKHKTQEYENVQINNFSSSWSDGLAFCALIHHFLPDAFDYTTLTKQTRRHNFELAFSVA 1668

HSP 2 Score: 36.1946 bits (82), Expect = 5.906e-25
Identity = 14/36 (38.89%), Postives = 23/36 (63.89%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKS 2009
            AGI PL++  D+  +  PD KCV+ Y+Q +Y   ++
Sbjct: 1672 AGIAPLLDVEDMVEMSRPDWKCVFVYVQSIYRRFRN 1707
[Close]
BLAST of Cytospin-A vs. Ensembl Fly
Match: CG34417 (gene:FBgn0085446 transcript:FBtr0301323)

HSP 1 Score: 99.7525 bits (247), Expect = 6.138e-25
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1645 LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            +KD LL W +  TQEY+NV+I N+S SW+DGLAFCALIHHF P++FDY+ L+      NF LAF  A
Sbjct: 3434 VKDQLLQWCKHKTQEYENVQINNFSSSWSDGLAFCALIHHFLPDAFDYTTLTKQTRRHNFELAFSVA 3500

HSP 2 Score: 35.039 bits (79), Expect = 6.138e-25
Identity = 14/36 (38.89%), Postives = 23/36 (63.89%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKS 2009
            AGI PL++  D+  +  PD KCV+ Y+Q +Y   ++
Sbjct: 3504 AGIAPLLDVEDMVEMSRPDWKCVFVYVQSIYRRFRN 3539
[Close]
BLAST of Cytospin-A vs. Ensembl Fly
Match: CG34417 (gene:FBgn0085446 transcript:FBtr0303205)

HSP 1 Score: 100.138 bits (248), Expect = 6.246e-25
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1645 LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            +KD LL W +  TQEY+NV+I N+S SW+DGLAFCALIHHF P++FDY+ L+      NF LAF  A
Sbjct: 3222 VKDQLLQWCKHKTQEYENVQINNFSSSWSDGLAFCALIHHFLPDAFDYTTLTKQTRRHNFELAFSVA 3288

HSP 2 Score: 34.6538 bits (78), Expect = 6.246e-25
Identity = 14/36 (38.89%), Postives = 23/36 (63.89%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKS 2009
            AGI PL++  D+  +  PD KCV+ Y+Q +Y   ++
Sbjct: 3292 AGIAPLLDVEDMVEMSRPDWKCVFVYVQSIYRRFRN 3327
[Close]
BLAST of Cytospin-A vs. Ensembl Zebrafish
Match: smtnb (smoothelin b [Source:ZFIN;Acc:ZDB-GENE-070912-2])

HSP 1 Score: 91.2781 bits (225), Expect = 1.900e-24
Identity = 36/69 (52.17%), Postives = 51/69 (73.91%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++K +LL W    T+ Y+NV I N+S SW+DG+AFCAL+H+F+PE+FDYS LS  +  +NF  AF TA+
Sbjct:  291 SIKQMLLDWCRSKTRSYENVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLSPTNRRQNFETAFSTAE 359

HSP 2 Score: 42.3578 bits (98), Expect = 1.900e-24
Identity = 18/37 (48.65%), Postives = 25/37 (67.57%), Query Frame = 3 
Query: 1899 YAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            YA   PL+E  D+ I+G  PD KCV+TY+Q + N L+
Sbjct:  388 YANCMPLLEVEDMMIMGKKPDSKCVFTYVQSLVNHLR 424
[Close]
BLAST of Cytospin-A vs. Ensembl Zebrafish
Match: smtnl1 (smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306-23])

HSP 1 Score: 98.2117 bits (243), Expect = 5.014e-22
Identity = 38/78 (48.72%), Postives = 55/78 (70.51%), Query Frame = 1 
Query: 1621 VGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFI 1854
            VG     ++K  +L W +  TQ+Y+ V I N+S SW+DGLAFCAL+H F+P +FD+S L +++  KNFTLAF TA+ +
Sbjct:  221 VGIAGGASIKQRILNWCKSKTQKYEGVSIDNFSSSWSDGLAFCALVHRFFPSAFDFSTLKASEREKNFTLAFSTAETL 298
[Close]
BLAST of Cytospin-A vs. Ensembl Zebrafish
Match: smtnl1 (smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306-23])

HSP 1 Score: 98.2117 bits (243), Expect = 6.478e-22
Identity = 38/78 (48.72%), Postives = 55/78 (70.51%), Query Frame = 1 
Query: 1621 VGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFI 1854
            VG     ++K  +L W +  TQ+Y+ V I N+S SW+DGLAFCAL+H F+P +FD+S L +++  KNFTLAF TA+ +
Sbjct:  216 VGIAGGASIKQRILNWCKSKTQKYEGVSIDNFSSSWSDGLAFCALVHRFFPSAFDFSTLKASEREKNFTLAFSTAETL 293

HSP 2 Score: 26.9498 bits (58), Expect = 6.478e-22
Identity = 13/31 (41.94%), Postives = 22/31 (70.97%), Query Frame = 3 
Query: 1914 PLIEPGDVNILG-SPDEKCVYTYIQFMYNSL 2003
            PL+E  D+ ++G +PD  CV+TY+Q + + L
Sbjct:  298 PLLEVSDMLLMGKNPDPLCVFTYVQALCHHL 328
[Close]
BLAST of Cytospin-A vs. Ensembl Zebrafish
Match: smtnl (smoothelin, like [Source:ZFIN;Acc:ZDB-GENE-040212-1])

HSP 1 Score: 86.6557 bits (213), Expect = 7.357e-22
Identity = 42/105 (40.00%), Postives = 58/105 (55.24%), Query Frame = 1 
Query: 1573 RKISQQIDPSNKIDPK-------------VGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            R I +++D  N   PK              G  +  ++K +LL W    T  YQN+ I N+S SW+DG+AFCAL+H F+P  FDY+ LS  +   NF LAF TA+
Sbjct:  348 RSIFERLDSENNSRPKPVDSKPKLKRSQSFGVSSASSIKQILLEWCRSKTIGYQNIDIQNFSSSWSDGMAFCALVHSFFPTEFDYNGLSPAERKHNFELAFSTAE 452

HSP 2 Score: 38.1206 bits (87), Expect = 7.357e-22
Identity = 20/52 (38.46%), Postives = 30/52 (57.69%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            F + FS     +    AG + LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  444 FELAFS-----TAEKKAGCDRLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 490
[Close]
BLAST of Cytospin-A vs. Ensembl Zebrafish
Match: smtnl (smoothelin, like [Source:ZFIN;Acc:ZDB-GENE-040212-1])

HSP 1 Score: 85.5001 bits (210), Expect = 1.467e-21
Identity = 41/105 (39.05%), Postives = 58/105 (55.24%), Query Frame = 1 
Query: 1573 RKISQQIDPSNKIDPK-------------VGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            R I +++D  N   PK              G  +  ++K +LL W    T  YQN+ I N+S SW+DG+AFCAL+H F+P  FDY+ L+  +   NF LAF TA+
Sbjct:  348 RSIFERLDSENNSRPKPVDSKPKLKRSQSFGVSSASSIKQILLEWCRSKTIGYQNIDIQNFSSSWSDGMAFCALVHSFFPTEFDYNGLTPAERKHNFELAFSTAE 452

HSP 2 Score: 38.1206 bits (87), Expect = 1.467e-21
Identity = 20/52 (38.46%), Postives = 30/52 (57.69%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLK 2006
            F + FS     +    AG + LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  444 FELAFS-----TAEKKAGCDRLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 490
[Close]
BLAST of Cytospin-A vs. Ensembl Xenopus
Match: smtn (ES1 protein homolog, mitochondrial-like [Source:Xenbase;Acc:XB-GENE-5871768])

HSP 1 Score: 91.6633 bits (226), Expect = 4.999e-25
Identity = 34/72 (47.22%), Postives = 53/72 (73.61%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFIF 1857
            ++K +LL W +  T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY+ L+  +  KNF LAF  A+ + 
Sbjct:  934 SIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEMLV 1005

HSP 2 Score: 44.2838 bits (103), Expect = 4.999e-25
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query: 1914 PLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSH 2012
            PL+E  D+ I+G  PD KCV+TY+Q +YN L+ H
Sbjct: 1009 PLVEVEDMMIMGKRPDSKCVFTYVQSLYNHLRRH 1042
[Close]
BLAST of Cytospin-A vs. Ensembl Xenopus
Match: smtn (ES1 protein homolog, mitochondrial-like [Source:Xenbase;Acc:XB-GENE-5871768])

HSP 1 Score: 91.6633 bits (226), Expect = 5.614e-25
Identity = 34/72 (47.22%), Postives = 53/72 (73.61%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFIF 1857
            ++K +LL W +  T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY+ L+  +  KNF LAF  A+ + 
Sbjct:  885 SIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEMLV 956

HSP 2 Score: 44.2838 bits (103), Expect = 5.614e-25
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query: 1914 PLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSH 2012
            PL+E  D+ I+G  PD KCV+TY+Q +YN L+ H
Sbjct:  960 PLVEVEDMMIMGKRPDSKCVFTYVQSLYNHLRRH 993
[Close]
BLAST of Cytospin-A vs. Ensembl Xenopus
Match: smtn (ES1 protein homolog, mitochondrial-like [Source:Xenbase;Acc:XB-GENE-5871768])

HSP 1 Score: 91.2781 bits (225), Expect = 6.196e-25
Identity = 34/72 (47.22%), Postives = 53/72 (73.61%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFIF 1857
            ++K +LL W +  T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY+ L+  +  KNF LAF  A+ + 
Sbjct:  887 SIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEMLV 958

HSP 2 Score: 44.2838 bits (103), Expect = 6.196e-25
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query: 1914 PLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSH 2012
            PL+E  D+ I+G  PD KCV+TY+Q +YN L+ H
Sbjct:  962 PLVEVEDMMIMGKRPDSKCVFTYVQSLYNHLRRH 995
[Close]
BLAST of Cytospin-A vs. Ensembl Xenopus
Match: smtn (ES1 protein homolog, mitochondrial-like [Source:Xenbase;Acc:XB-GENE-5871768])

HSP 1 Score: 91.6633 bits (226), Expect = 7.131e-25
Identity = 34/72 (47.22%), Postives = 53/72 (73.61%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFIF 1857
            ++K +LL W +  T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY+ L+  +  KNF LAF  A+ + 
Sbjct:  844 SIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAEMLV 915

HSP 2 Score: 43.8986 bits (102), Expect = 7.131e-25
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query: 1914 PLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSH 2012
            PL+E  D+ I+G  PD KCV+TY+Q +YN L+ H
Sbjct:  919 PLVEVEDMMIMGKRPDSKCVFTYVQSLYNHLRRH 952
[Close]
BLAST of Cytospin-A vs. Ensembl Xenopus
Match: smtn (ES1 protein homolog, mitochondrial-like [Source:Xenbase;Acc:XB-GENE-5871768])

HSP 1 Score: 90.1225 bits (222), Expect = 5.775e-21
Identity = 34/69 (49.28%), Postives = 52/69 (75.36%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++K +LL W +  T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY+ L+  +  KNF LAF  A+
Sbjct:  934 SIKQMLLDWCKAKTRGYEHVNIQNFSSSWSDGMAFCALVHNFFPEAFDYNQLNPQNRRKNFDLAFSAAE 1002

HSP 2 Score: 31.9574 bits (71), Expect = 5.775e-21
Identity = 15/39 (38.46%), Postives = 21/39 (53.85%), Query Frame = 3 
Query: 1887 SCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSL 2003
            +   +A    L++  D+  +  PD KCVYTYIQ  Y  L
Sbjct: 1000 AAEKHADCPQLLDAEDMVRMREPDWKCVYTYIQEFYRCL 1038
[Close]
BLAST of Cytospin-A vs. Ensembl Mouse
Match: Smtn (smoothelin [Source:MGI Symbol;Acc:MGI:1354727])

HSP 1 Score: 90.1225 bits (222), Expect = 3.889e-24
Identity = 33/69 (47.83%), Postives = 51/69 (73.91%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++K +LL W    T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY  LS  +  +NF +AF +A+
Sbjct:  806 SIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEMAFSSAE 874

HSP 2 Score: 42.3578 bits (98), Expect = 3.889e-24
Identity = 18/34 (52.94%), Postives = 25/34 (73.53%), Query Frame = 3 
Query: 1914 PLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSH 2012
            PL+E  D+ I+G  PD KCV+TY+Q +YN L+ H
Sbjct:  904 PLVEVEDMMIMGKKPDPKCVFTYVQSLYNHLRRH 937
[Close]
BLAST of Cytospin-A vs. Ensembl Mouse
Match: Smtnl2 (smoothelin-like 2 [Source:MGI Symbol;Acc:MGI:2442764])

HSP 1 Score: 87.4261 bits (215), Expect = 2.745e-22
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1 
Query: 1624 GKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            G  +  ++K +LL W    T  YQ+V + N+S SW+DG+AFCAL+H F+P++FDY+ LS     KNF LAF  A+
Sbjct:  307 GVASASSIKQILLEWCRSKTVGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNALSPTQRQKNFELAFTMAE 381

HSP 2 Score: 38.891 bits (89), Expect = 2.745e-22
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1896 NYAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            N A  E LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  382 NLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 419
[Close]
BLAST of Cytospin-A vs. Ensembl Mouse
Match: Smtnl2 (smoothelin-like 2 [Source:MGI Symbol;Acc:MGI:2442764])

HSP 1 Score: 87.4261 bits (215), Expect = 3.050e-22
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1 
Query: 1624 GKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            G  +  ++K +LL W    T  YQ+V + N+S SW+DG+AFCAL+H F+P++FDY+ LS     KNF LAF  A+
Sbjct:  341 GVASASSIKQILLEWCRSKTVGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNALSPTQRQKNFELAFTMAE 415

HSP 2 Score: 38.5058 bits (88), Expect = 3.050e-22
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1896 NYAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            N A  E LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  416 NLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 453
[Close]
BLAST of Cytospin-A vs. Ensembl Mouse
Match: Smtnl1 (smoothelin-like 1 [Source:MGI Symbol;Acc:MGI:1915928])

HSP 1 Score: 88.9669 bits (219), Expect = 3.151e-22
Identity = 38/72 (52.78%), Postives = 51/72 (70.83%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLK---NFTLAFETAK 1848
             +K++LL W    T+ Y++V I N+S SW+ G+AFCALIH F+PE+FDY+ L   DP K   NFTLAF TA+
Sbjct:  344 GVKNMLLEWCRAMTRNYEHVDIQNFSSSWSSGMAFCALIHKFFPEAFDYAEL---DPAKRRHNFTLAFSTAE 412

HSP 2 Score: 36.965 bits (84), Expect = 3.151e-22
Identity = 21/50 (42.00%), Postives = 26/50 (52.00%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSL 2003
            F + FS     +    A    L+E  D+  L  PD KCVYTYIQ +Y SL
Sbjct:  404 FTLAFS-----TAEKLADCAQLLEVDDMVRLAVPDSKCVYTYIQELYRSL 448
[Close]
BLAST of Cytospin-A vs. Ensembl Mouse
Match: Smtn (smoothelin [Source:MGI Symbol;Acc:MGI:1354727])

HSP 1 Score: 90.1225 bits (222), Expect = 1.911e-21
Identity = 33/69 (47.83%), Postives = 51/69 (73.91%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++K +LL W    T+ Y++V I N+S SW+DG+AFCAL+H+F+PE+FDY  LS  +  +NF +AF +A+
Sbjct:  806 SIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEMAFSSAE 874

HSP 2 Score: 33.113 bits (74), Expect = 1.911e-21
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSL 2003
            F + FS     S   +A    L++  D+  L  PD KCVYTYIQ  Y  L
Sbjct:  866 FEMAFS-----SAETHADCPQLLDTEDMVRLREPDWKCVYTYIQEFYRCL 910
[Close]
BLAST of Cytospin-A vs. UniProt/SwissProt
Match: sp|Q8CI12|SMTL2_MOUSE (Smoothelin-like protein 2 OS=Mus musculus OX=10090 GN=Smtnl2 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.855e-21
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1 
Query: 1624 GKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            G  +  ++K +LL W    T  YQ+V + N+S SW+DG+AFCAL+H F+P++FDY+ LS     KNF LAF  A+
Sbjct:  341 GVASASSIKQILLEWCRSKTVGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNALSPTQRQKNFELAFTMAE 415

HSP 2 Score: 38.5058 bits (88), Expect = 1.855e-21
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1896 NYAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            N A  E LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  416 NLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 453
[Close]
BLAST of Cytospin-A vs. UniProt/SwissProt
Match: sp|Q99LM3|SMTL1_MOUSE (Smoothelin-like protein 1 OS=Mus musculus OX=10090 GN=Smtnl1 PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.917e-21
Identity = 38/72 (52.78%), Postives = 51/72 (70.83%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLK---NFTLAFETAK 1848
             +K++LL W    T+ Y++V I N+S SW+ G+AFCALIH F+PE+FDY+ L   DP K   NFTLAF TA+
Sbjct:  344 GVKNMLLEWCRAMTRNYEHVDIQNFSSSWSSGMAFCALIHKFFPEAFDYAEL---DPAKRRHNFTLAFSTAE 412

HSP 2 Score: 36.965 bits (84), Expect = 1.917e-21
Identity = 21/50 (42.00%), Postives = 26/50 (52.00%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSL 2003
            F + FS     +    A    L+E  D+  L  PD KCVYTYIQ +Y SL
Sbjct:  404 FTLAFS-----TAEKLADCAQLLEVDDMVRLAVPDSKCVYTYIQELYRSL 448
[Close]
BLAST of Cytospin-A vs. UniProt/SwissProt
Match: sp|Q2TAL5|SMTL2_HUMAN (Smoothelin-like protein 2 OS=Homo sapiens OX=9606 GN=SMTNL2 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.218e-21
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1 
Query: 1624 GKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            G  +  ++K +LL W    T  YQ+V + N+S SW+DG+AFCAL+H F+P++FDY+ LS     KNF LAF  A+
Sbjct:  346 GVASASSIKQILLEWCRSKTLGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNSLSPTQRQKNFELAFTMAE 420

HSP 2 Score: 38.891 bits (89), Expect = 4.218e-21
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1896 NYAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            N A  E LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  421 NLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 458
[Close]
BLAST of Cytospin-A vs. UniProt/SwissProt
Match: sp|Q2KI85|SMTL2_BOVIN (Smoothelin-like protein 2 OS=Bos taurus OX=9913 GN=SMTNL2 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.580e-21
Identity = 34/75 (45.33%), Postives = 50/75 (66.67%), Query Frame = 1 
Query: 1624 GKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            G  +  ++K +LL W    T  YQ+V + N+S SW+DG+AFCAL+H F+P++FDY+ LS     +NF LAF  A+
Sbjct:  343 GVASASSIKQILLEWCRSKTLGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNALSPTQRRQNFELAFTMAE 417

HSP 2 Score: 38.891 bits (89), Expect = 4.580e-21
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 3 
Query: 1896 NYAGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            N A  E LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  418 NLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 455
[Close]
BLAST of Cytospin-A vs. UniProt/SwissProt
Match: sp|Q2KN97|CYTSA_CHICK (Cytospin-A OS=Gallus gallus OX=9031 GN=SPECC1L PE=2 SV=2)

HSP 1 Score: 98.5969 bits (244), Expect = 1.109e-19
Identity = 62/199 (31.16%), Postives = 100/199 (50.25%), Query Frame = 1 
Query: 1309 MSASKPIPQM-----STPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPN--LNRGASNEKNL--------TVPEGRSRKISQQIDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFIFI 1860
            +SASKP+  +     S  EIP ++++ R SS       P  P+   ++  S   S +   +I   +  P+   P   +  G ++ +            P  RSR   ++ DP + +  + G     + ++ LL W +K T+ YQN+ ITN+S SW DGLAFCA++H + P    Y  L+S D  +NFTLAF+ A+ + I
Sbjct:  893 ISASKPLATLTDKRPSYAEIPVQEHLLRTSSTSRPASLPRVPAM--ESAKSISVSRRSSEEIKRDISAPDGASPASLMAMGTTSPQLSLSSSPTASVTPTTRSRIREERKDPLSALAREYGG----SKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCAVLHTYLPAHIPYQELNSQDKRRNFTLAFQAAESVGI 1085
[Close]
BLAST of Cytospin-A vs. TrEMBL
Match: T1ES98 (Calponin-homology (CH) domain-containing protein OS=Helobdella robusta OX=6412 GN=20199448 PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 7.429e-24
Identity = 43/68 (63.24%), Postives = 55/68 (80.88%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            A+K++L+ W +  T+EY+NV ITN+S SWADG+AFCALIHHFYPESFDYS L+  +   NFTLAF+ A
Sbjct:  122 AIKEMLIKWCQMQTKEYENVNITNFSSSWADGMAFCALIHHFYPESFDYSKLNPKNRRGNFTLAFDVA 189

HSP 2 Score: 36.1946 bits (82), Expect = 7.429e-24
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSL 2003
            A I PL+E  D+ ++  PD KCV+TY+Q  Y   
Sbjct:  193 ANIAPLLEVDDMVMMKVPDWKCVFTYVQSFYRRF 226
[Close]
BLAST of Cytospin-A vs. TrEMBL
Match: A0A226E3T5 (Smoothelin OS=Folsomia candida OX=158441 GN=Fcan01_12210 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.411e-23
Identity = 39/66 (59.09%), Postives = 49/66 (74.24%), Query Frame = 1 
Query: 1648 KDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            KD+LL W +  T++Y+NVKI N+S SW+DGLAFCALIHHFYP++FDY  L   +   NF LAF  A
Sbjct: 2103 KDILLYWCQCRTRDYENVKIENFSSSWSDGLAFCALIHHFYPDAFDYKSLDPKNRRFNFDLAFRVA 2168

HSP 2 Score: 43.5134 bits (101), Expect = 1.411e-23
Identity = 20/36 (55.56%), Postives = 27/36 (75.00%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            AGI PL++  D+ ++G  PD KCV+TY+Q MY SLK
Sbjct: 2172 AGIYPLLDTEDMIVMGRRPDWKCVFTYVQSMYRSLK 2207
[Close]
BLAST of Cytospin-A vs. TrEMBL
Match: A0A4W5RYX6 (Calponin-homology (CH) domain-containing protein OS=Hucho hucho OX=62062 PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.855e-23
Identity = 41/93 (44.09%), Postives = 59/93 (63.44%), Query Frame = 1 
Query: 1597 PSNKIDPKVGKITKLA---------LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            P NK   KV  + + A         +K +LL W    T+ Y+NV I N+S SW+DG+AFCAL+H+F+PE+FDYS L++ +   NF +AF TA+
Sbjct:  954 PGNKAVAKVNMVQRSASCVVPNANSIKQMLLDWCRAKTRSYENVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTAANRRHNFEVAFSTAE 1046

HSP 2 Score: 44.669 bits (104), Expect = 1.855e-23
Identity = 21/44 (47.73%), Postives = 28/44 (63.64%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSHPRNSAK 2030
            A   PL+E  D+ I+G  PD KCV+TY+Q + N L+ H  N AK
Sbjct: 1049 ANCMPLLEVEDMMIMGKKPDSKCVFTYVQSLVNHLRRHEMNLAK 1092
[Close]
BLAST of Cytospin-A vs. TrEMBL
Match: A0A1S3PGI1 (smoothelin-like isoform X6 OS=Salmo salar OX=8030 GN=LOC106585267 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.266e-23
Identity = 41/93 (44.09%), Postives = 59/93 (63.44%), Query Frame = 1 
Query: 1597 PSNKIDPKVGKITKLA---------LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            P NK   KV  + + A         +K +LL W    T+ Y+NV I N+S SW+DG+AFCAL+H+F+PE+FDYS L++ +   NF +AF TA+
Sbjct:  797 PGNKAVAKVNMVQRSASCVVPNANSIKQMLLDWCRAKTRSYENVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTAANRRHNFEVAFSTAE 889

HSP 2 Score: 45.0542 bits (105), Expect = 2.266e-23
Identity = 24/60 (40.00%), Postives = 33/60 (55.00%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLKSHPRNSAK 2030
            F + FS     +    A   PL+E  D+ I+G  PD KCV+TY+Q + N L+ H  N AK
Sbjct:  881 FEVAFS-----TAEECANCMPLLEVEDMMIMGRKPDSKCVFTYVQSLVNHLRRHEMNLAK 935
[Close]
BLAST of Cytospin-A vs. TrEMBL
Match: A0A1S3PGP7 (smoothelin-like isoform X4 OS=Salmo salar OX=8030 GN=LOC106585267 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.798e-23
Identity = 41/93 (44.09%), Postives = 59/93 (63.44%), Query Frame = 1 
Query: 1597 PSNKIDPKVGKITKLA---------LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            P NK   KV  + + A         +K +LL W    T+ Y+NV I N+S SW+DG+AFCAL+H+F+PE+FDYS L++ +   NF +AF TA+
Sbjct:  986 PGNKAVAKVNMVQRSASCVVPNANSIKQMLLDWCRAKTRSYENVDIQNFSSSWSDGMAFCALVHNFFPEAFDYSSLTAANRRHNFEVAFSTAE 1078

HSP 2 Score: 44.2838 bits (103), Expect = 2.798e-23
Identity = 21/44 (47.73%), Postives = 28/44 (63.64%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLKSHPRNSAK 2030
            A   PL+E  D+ I+G  PD KCV+TY+Q + N L+ H  N AK
Sbjct: 1081 ANCMPLLEVEDMMIMGRKPDSKCVFTYVQSLVNHLRRHEMNLAK 1124
[Close]
BLAST of Cytospin-A vs. Ensembl Cavefish
Match: smtnl (smoothelin like 2 [Source:NCBI gene;Acc:103036153])

HSP 1 Score: 88.9669 bits (219), Expect = 4.962e-23
Identity = 36/69 (52.17%), Postives = 47/69 (68.12%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++K +LL W    T  YQN+ I N+S SW+DG+AFCAL+H F+P  FDY+ LS  D   NF LAF TA+
Sbjct:  395 SIKQILLEWCRSKTIGYQNIDIQNFSSSWSDGMAFCALVHSFFPTEFDYNALSPVDRKHNFELAFSTAE 463

HSP 2 Score: 39.2762 bits (90), Expect = 4.962e-23
Identity = 20/52 (38.46%), Postives = 31/52 (59.62%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLK 2006
            F + FS     +   +AG + LIE  D+ ++G  PD  CV+TY+Q +YN L+
Sbjct:  455 FELAFS-----TAEKHAGCDRLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLR 501
[Close]
BLAST of Cytospin-A vs. Ensembl Cavefish
Match: ENSAMXT00000056105.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000095.1:180394:186664:-1 gene:ENSAMXG00000031006.1 transcript:ENSAMXT00000056105.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.2041 bits (230), Expect = 1.320e-21
Identity = 35/68 (51.47%), Postives = 54/68 (79.41%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            ++K +LL W    T++Y+NV I N+S SW+DG+AFCAL+H+F+PE+FDY+ LS ++  +NF +AF+TA
Sbjct:  200 SIKQMLLDWCRAKTRDYENVDIQNFSSSWSDGMAFCALVHNFFPEAFDYAALSPSNRRQNFEVAFKTA 267

HSP 2 Score: 30.4166 bits (67), Expect = 1.320e-21
Identity = 13/29 (44.83%), Postives = 18/29 (62.07%), Query Frame = 3 
Query: 1917 LIEPGDVNILGSPDEKCVYTYIQFMYNSL 2003
            L++  D+  +  PD KCVYTY+Q  Y  L
Sbjct:  275 LLDVDDMVRMREPDWKCVYTYLQEFYRGL 303
[Close]
BLAST of Cytospin-A vs. Ensembl Cavefish
Match: smtnl1 (smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306-23])

HSP 1 Score: 96.6709 bits (239), Expect = 1.225e-20
Identity = 37/78 (47.44%), Postives = 54/78 (69.23%), Query Frame = 1 
Query: 1621 VGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFI 1854
            VG     ++K  +L W    T++Y+ V I N+S SW+DGLAFCAL+H F+P +FD+S L +++  KNFTLAF TA+ +
Sbjct:  254 VGVSGGASIKQKILQWCSNKTRDYEGVSIENFSSSWSDGLAFCALVHRFFPTAFDFSTLKASEREKNFTLAFSTAETL 331
[Close]
BLAST of Cytospin-A vs. Ensembl Cavefish
Match: smtnl1 (smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306-23])

HSP 1 Score: 95.9005 bits (237), Expect = 1.431e-20
Identity = 37/78 (47.44%), Postives = 54/78 (69.23%), Query Frame = 1 
Query: 1621 VGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFI 1854
            VG     ++K  +L W    T++Y+ V I N+S SW+DGLAFCAL+H F+P +FD+S L +++  KNFTLAF TA+ +
Sbjct:  217 VGVSGGASIKQKILQWCSNKTRDYEGVSIENFSSSWSDGLAFCALVHRFFPTAFDFSTLKASEREKNFTLAFSTAETL 294
[Close]
BLAST of Cytospin-A vs. Ensembl Cavefish
Match: smtnl1 (smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306-23])

HSP 1 Score: 96.2857 bits (238), Expect = 1.568e-20
Identity = 37/78 (47.44%), Postives = 54/78 (69.23%), Query Frame = 1 
Query: 1621 VGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFI 1854
            VG     ++K  +L W    T++Y+ V I N+S SW+DGLAFCAL+H F+P +FD+S L +++  KNFTLAF TA+ +
Sbjct:  254 VGVSGGASIKQKILQWCSNKTRDYEGVSIENFSSSWSDGLAFCALVHRFFPTAFDFSTLKASEREKNFTLAFSTAETL 331
[Close]
BLAST of Cytospin-A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008014.1 (pep scaffold:Pmarinus_7.0:GL480769:7574:15080:-1 gene:ENSPMAG00000007250.1 transcript:ENSPMAT00000008014.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 77.411 bits (189), Expect = 3.363e-19
Identity = 32/65 (49.23%), Postives = 45/65 (69.23%), Query Frame = 1 
Query: 1654 LLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            L L W +  T+ Y NV + N+S SW DG+AFCAL+H ++PE+FD++ LS  +P  NF LAF  A+
Sbjct:   23 LTLEWCKTRTENYANVSVRNFSSSWCDGMAFCALVHSYFPEAFDFAELSPQNPRHNFQLAFTCAE 87

HSP 2 Score: 35.4242 bits (80), Expect = 3.363e-19
Identity = 15/33 (45.45%), Postives = 25/33 (75.76%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYN 1997
            A  +PL++  D+ I+G+ PD KCV+TY+Q ++N
Sbjct:   90 AHCDPLLDVEDMIIMGNRPDSKCVFTYVQSLFN 122
[Close]
BLAST of Cytospin-A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009649.1 (pep scaffold:Pmarinus_7.0:GL478928:16819:21705:-1 gene:ENSPMAG00000008722.1 transcript:ENSPMAT00000009649.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.8626 bits (172), Expect = 2.752e-18
Identity = 29/52 (55.77%), Postives = 39/52 (75.00%), Query Frame = 1 
Query: 1693 QNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            QNV I N+S SW DG+AFCAL+H ++PE+FD++ LS  +P  NF LAF  A+
Sbjct:    1 QNVTIKNFSSSWCDGMAFCALVHSYFPEAFDFAKLSPQNPRHNFQLAFTCAE 52

HSP 2 Score: 39.2762 bits (90), Expect = 2.752e-18
Identity = 17/40 (42.50%), Postives = 27/40 (67.50%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILGS-PDEKCVYTYIQFMYNSLKSHPR 2018
            A  +PL++  D+ ++G  PD KCV+TY+Q +YN L+   R
Sbjct:   55 AHCDPLLDVDDMIMMGKKPDSKCVFTYVQSLYNKLRRLER 94
[Close]
BLAST of Cytospin-A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000684.1 (pep scaffold:Pmarinus_7.0:GL483163:49540:93186:-1 gene:ENSPMAG00000000623.1 transcript:ENSPMAT00000000684.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.707 bits (169), Expect = 1.069e-14
Identity = 31/67 (46.27%), Postives = 42/67 (62.69%), Query Frame = 1 
Query: 1648 KDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            K+ LL+W ++ T  Y+NV + N+  SW DGLAF ALIH   P+  DY  L  +DP+ N   AFE A+
Sbjct:  116 KEGLLLWCQRKTAPYKNVNVQNFHTSWKDGLAFNALIHRHRPDLIDYDKLRKDDPIGNLNNAFEVAE 182

HSP 2 Score: 28.1054 bits (61), Expect = 1.069e-14
Identity = 15/53 (28.30%), Postives = 26/53 (49.06%), Query Frame = 3 
Query: 1899 YAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS*NYV 2057
            Y  I  +++  D+     PDEK + TY+   Y     H  + A++AE + N +
Sbjct:  184 YLDIPKMLDAEDIVNTARPDEKAIMTYVSCYY-----HAFSGAQKAETAANRI 231
[Close]
BLAST of Cytospin-A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009295.1 (pep scaffold:Pmarinus_7.0:GL479988:2885:20778:1 gene:ENSPMAG00000008383.1 transcript:ENSPMAT00000009295.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.3218 bits (168), Expect = 1.337e-14
Identity = 31/67 (46.27%), Postives = 42/67 (62.69%), Query Frame = 1 
Query: 1648 KDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            K+ LL+W ++ T  Y+NV + N+  SW DGLAF ALIH   P+  DY  L  +DP+ N   AFE A+
Sbjct:  112 KEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPDLIDYEKLRKDDPITNLNNAFEVAE 178

HSP 2 Score: 28.1054 bits (61), Expect = 1.337e-14
Identity = 15/53 (28.30%), Postives = 26/53 (49.06%), Query Frame = 3 
Query: 1899 YAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS*NYV 2057
            Y  I  +++  D+     PDEK + TY+   Y     H  + A++AE + N +
Sbjct:  180 YLDIPKMLDAEDIVGTARPDEKAIMTYVSCYY-----HAFSGAQKAETAANRI 227
[Close]
BLAST of Cytospin-A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004049.1 (pep scaffold:Pmarinus_7.0:GL476488:489896:554954:1 gene:ENSPMAG00000003699.1 transcript:ENSPMAT00000004049.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 66.2402 bits (160), Expect = 2.087e-12
Identity = 30/67 (44.78%), Postives = 39/67 (58.21%), Query Frame = 1 
Query: 1648 KDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            KD LL+W +  T  Y NV++ N++ SW DGLAF ALIH   P+  D+  L   DP  N   AF  A+
Sbjct:  128 KDALLLWCQMKTAGYPNVRVHNFTTSWRDGLAFNALIHKHRPDLIDFDALDPADPYGNMQTAFNVAE 194

HSP 2 Score: 23.8682 bits (50), Expect = 2.087e-12
Identity = 10/26 (38.46%), Postives = 16/26 (61.54%), Query Frame = 3 
Query: 1905 GIEPLIEPGDVNILGSPDEKCVYTYI 1982
            G+  L++  D+ +   PDEK V TY+
Sbjct:  198 GLTQLLDAEDIAV-EHPDEKSVITYV 222
[Close]
BLAST of Cytospin-A vs. Ensembl Nematostella
Match: EDO36878 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHE7])

HSP 1 Score: 79.337 bits (194), Expect = 3.084e-16
Identity = 34/73 (46.58%), Postives = 49/73 (67.12%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFIFI 1860
            ++  L+L W +  T++Y  V+I N+S S+ DGLAFCALIH F P+ FD+S L+  D   N+TLA ET + + I
Sbjct:  174 SVSSLVLTWCQDVTKDYPGVEIKNFSTSFYDGLAFCALIHKFNPDKFDFSELTPEDRRHNWTLALETGESVGI 246
[Close]
BLAST of Cytospin-A vs. Ensembl Nematostella
Match: EDO36877 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHE6])

HSP 1 Score: 72.4034 bits (176), Expect = 6.997e-16
Identity = 33/71 (46.48%), Postives = 48/71 (67.61%), Query Frame = 1 
Query: 1636 KLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            ++++  L+L W +  T+ Y+ V ITN+S S+A+GLAFCALIH F P+ FD+  L   +   NF LAFET +
Sbjct:    1 QMSVSSLILTWCKDVTEGYKGVNITNFSGSFANGLAFCALIHKFNPDKFDFDSLDPENRHYNFKLAFETGE 71
[Close]
BLAST of Cytospin-A vs. Ensembl Nematostella
Match: ALPHA-ACTININ (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4X0])

HSP 1 Score: 73.559 bits (179), Expect = 1.121e-15
Identity = 32/67 (47.76%), Postives = 43/67 (64.18%), Query Frame = 1 
Query: 1648 KDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            KD LL+W ++ T  Y+NV + N++ S+ DGLAFCALIH   P+  DY  L   DPL N   AF+ A+
Sbjct:  143 KDGLLLWCQRKTAPYKNVSVKNFTTSFKDGLAFCALIHRHRPDLIDYDSLRKEDPLYNLNKAFDVAE 209

HSP 2 Score: 28.4906 bits (62), Expect = 1.121e-15
Identity = 17/58 (29.31%), Postives = 30/58 (51.72%), Query Frame = 3 
Query: 1866 FSKIFIISCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPR--NSAKR 2033
             +K F ++   Y  I  +++P D+     PDE+ V TY+   Y++  S  +   +AKR
Sbjct:  201 LNKAFDVA-EQYLDIPRMLDPEDMVETERPDERAVMTYVSSYYHAFTSSQQADTAAKR 257
[Close]
BLAST of Cytospin-A vs. Ensembl Nematostella
Match: EDO36124 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJI6])

HSP 1 Score: 69.3218 bits (168), Expect = 2.566e-14
Identity = 30/63 (47.62%), Postives = 41/63 (65.08%), Query Frame = 1 
Query: 1657 LLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            +L W  + T+ ++ V+I N++ SWADGLA CAL H+F PE   Y  L   D  +N+TLAFE A
Sbjct:    1 MLEWCRRRTRGHEGVEIQNFTTSWADGLAMCALFHNFSPELIPYETLDPKDRTRNWTLAFEAA 63
[Close]
BLAST of Cytospin-A vs. Ensembl Nematostella
Match: EDO37708 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF38])

HSP 1 Score: 70.0922 bits (170), Expect = 4.189e-14
Identity = 35/84 (41.67%), Postives = 48/84 (57.14%), Query Frame = 1 
Query: 1603 NKIDPKVG--KITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            N +D ++   K+  L+ KD LL W +K TQ Y  V + N+S SW DG AF A+IH + P+  D+S LS   P  N   AF  A+
Sbjct:  142 NDLDVEIAGEKVQDLSAKDALLHWSKKVTQGYPKVDVKNFSSSWRDGFAFNAIIHRYRPDLVDFSKLSKTSPEANIEYAFHVAE 225

HSP 2 Score: 26.5646 bits (57), Expect = 4.189e-14
Identity = 11/33 (33.33%), Postives = 18/33 (54.55%), Query Frame = 3 
Query: 1917 LIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHP 2015
            L++  DV +  +PDEK + TY+  +Y      P
Sbjct:  233 LLDVEDVVVSENPDEKSIMTYVASLYEVFPRTP 265
[Close]
BLAST of Cytospin-A vs. Ensembl Medaka
Match: ENSORLT00000042079.1 (smoothelin [Source:NCBI gene;Acc:101173151])

HSP 1 Score: 90.5077 bits (223), Expect = 2.617e-23
Identity = 37/93 (39.78%), Postives = 59/93 (63.44%), Query Frame = 1 
Query: 1597 PSNKIDPKVGKITKL---------ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            P N++  KV ++ +          ++K +LL W    T+ Y++V I N+S SW+DG+AFCAL+H F+P++FDYS LS ++   NF +AF  A+
Sbjct:  783 PFNQVVAKVNRVQRSTSFGVPNANSIKQMLLDWCRAKTRSYEHVDIQNFSSSWSDGMAFCALVHSFFPDAFDYSCLSPSNRRHNFEVAFSAAE 875

HSP 2 Score: 38.891 bits (89), Expect = 2.617e-23
Identity = 16/32 (50.00%), Postives = 24/32 (75.00%), Query Frame = 3 
Query: 1914 PLIEPGDVNILGS-PDEKCVYTYIQFMYNSLK 2006
            PL+E  D+ I+G+ PD KCV+TY+Q + N L+
Sbjct:  882 PLLEVEDMMIMGNKPDSKCVFTYVQSLVNHLR 913
[Close]
BLAST of Cytospin-A vs. Ensembl Medaka
Match: smtna (smoothelin-like protein 1 [Source:NCBI gene;Acc:101166813])

HSP 1 Score: 88.9669 bits (219), Expect = 9.537e-23
Identity = 35/68 (51.47%), Postives = 49/68 (72.06%), Query Frame = 1 
Query: 1645 LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            +K +LL W    T+ Y+ V I N+S SW DG+AFCAL+H F+P++F+YS+L+ N P  NF LAF TA+
Sbjct:  124 VKQMLLDWCRAKTEPYEGVDIQNFSSSWKDGIAFCALVHRFFPDAFEYSILNPNKPRDNFQLAFSTAE 191

HSP 2 Score: 38.5058 bits (88), Expect = 9.537e-23
Identity = 20/50 (40.00%), Postives = 26/50 (52.00%), Query Frame = 3 
Query: 1854 FYILFSKIFIISCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSL 2003
            F + FS     +    AG  PL++  D+  +  PD KCVYTYIQ  Y  L
Sbjct:  183 FQLAFS-----TAEKMAGCPPLLDADDLVRMQEPDWKCVYTYIQEFYRCL 227
[Close]
BLAST of Cytospin-A vs. Ensembl Medaka
Match: smtnl1 (smoothelin like 1 [Source:NCBI gene;Acc:101166638])

HSP 1 Score: 93.5893 bits (231), Expect = 5.122e-22
Identity = 37/74 (50.00%), Postives = 53/74 (71.62%), Query Frame = 1 
Query: 1633 TKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFI 1854
            T  ++K  +L W    T+ Y++V I N+S SW DGLAFCALIH F+P++FDY+ L+  +  KNFTLAF+TA+ +
Sbjct:  228 TGASIKQKILQWCRSKTRNYKDVNIENFSSSWCDGLAFCALIHRFFPDAFDYNSLTPQEREKNFTLAFQTAESL 301

HSP 2 Score: 31.187 bits (69), Expect = 5.122e-22
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 3 
Query: 1914 PLIEPGDVNILG-SPDEKCVYTYIQFMYNSL 2003
            PL+E  D+ ++G SPD  CV+TY+Q + +SL
Sbjct:  306 PLLEVADMIMMGKSPDPMCVFTYVQSLCHSL 336
[Close]
BLAST of Cytospin-A vs. Ensembl Medaka
Match: smtnl1 (smoothelin like 1 [Source:NCBI gene;Acc:101166638])

HSP 1 Score: 93.5893 bits (231), Expect = 5.122e-22
Identity = 37/74 (50.00%), Postives = 53/74 (71.62%), Query Frame = 1 
Query: 1633 TKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAKFI 1854
            T  ++K  +L W    T+ Y++V I N+S SW DGLAFCALIH F+P++FDY+ L+  +  KNFTLAF+TA+ +
Sbjct:  228 TGASIKQKILQWCRSKTRNYKDVNIENFSSSWCDGLAFCALIHRFFPDAFDYNSLTPQEREKNFTLAFQTAESL 301

HSP 2 Score: 31.187 bits (69), Expect = 5.122e-22
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 3 
Query: 1914 PLIEPGDVNILG-SPDEKCVYTYIQFMYNSL 2003
            PL+E  D+ ++G SPD  CV+TY+Q + +SL
Sbjct:  306 PLLEVADMIMMGKSPDPMCVFTYVQSLCHSL 336
[Close]
BLAST of Cytospin-A vs. Ensembl Medaka
Match: smtnl (smoothelin like 2 [Source:NCBI gene;Acc:101158374])

HSP 1 Score: 83.5741 bits (205), Expect = 3.028e-21
Identity = 34/68 (50.00%), Postives = 46/68 (67.65%), Query Frame = 1 
Query: 1645 LKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            +K +LL W    T  YQN+ I N+S SW++G+AFCAL+H F+P  FDY+ LS  +   NF LAF TA+
Sbjct:  426 IKQILLEWCRSKTIGYQNIDIQNFSSSWSNGMAFCALVHSFFPAEFDYNSLSPANRRHNFELAFATAE 493

HSP 2 Score: 38.5058 bits (88), Expect = 3.028e-21
Identity = 18/36 (50.00%), Postives = 25/36 (69.44%), Query Frame = 3 
Query: 1902 AGIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLK 2006
            AG + LIE  D+ I+G  PD  CV+TY+Q +YN L+
Sbjct:  496 AGCDRLIEVDDMMIMGHKPDPMCVFTYVQSLYNHLR 531
[Close]
BLAST of Cytospin-A vs. Planmine SMEST
Match: SMESG000002849.1 (SMESG000002849.1)

HSP 1 Score: 955.666 bits (2469), Expect = 0.000e+0
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 1 
Query:  307 MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCXXXXXXXXXXXXXXXXXXXXXXXXLDXXXXXXXXXXXXXIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQIDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCLNRRRLLRKVLRSKSSNSSDISNNLDTKENTNETNQTKTIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQIDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK
Sbjct:    1 MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCLNRRRLLRKVLRSKSSNSSDISNNLDTKENTNETNQTKTIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQIDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 514

HSP 2 Score: 112.464 bits (280), Expect = 1.455e-25
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 3 
Query: 1887 SCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS 2045
            + +NYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS
Sbjct:  512 TAKNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS 564
[Close]
BLAST of Cytospin-A vs. Planmine SMEST
Match: SMESG000002849.1 (SMESG000002849.1)

HSP 1 Score: 936.406 bits (2419), Expect = 0.000e+0
Identity = 514/551 (93.28%), Postives = 514/551 (93.28%), Query Frame = 1 
Query:  307 MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCXXXXXXXXXXXXXXXXXXXXXXXXLDXXXXXXXXXXXXXIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQ-------------------------------------IDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCLNRRRLLRKVLRSKSSNSSDISNNLDTKENTNETNQTKTIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQ                                     IDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK
Sbjct:    1 MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCLNRRRLLRKVLRSKSSNSSDISNNLDTKENTNETNQTKTIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQNIIMMIPQMKKKAQIARSSVQVKKEQLLIFCQLNTQEIDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 551

HSP 2 Score: 112.464 bits (280), Expect = 1.657e-25
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 3 
Query: 1887 SCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS 2045
            + +NYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS
Sbjct:  549 TAKNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS 601
[Close]
BLAST of Cytospin-A vs. Planmine SMEST
Match: SMESG000002849.1 (SMESG000002849.1)

HSP 1 Score: 930.243 bits (2403), Expect = 0.000e+0
Identity = 506/517 (97.87%), Postives = 508/517 (98.26%), Query Frame = 1 
Query:  307 MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCXXXXXXXXXXXXXXXXXXXXXXXXLDXXXXXXXXXXXXXIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQIDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQNVKITNYSK---SWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 1848
            MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCLNRRRLLRKVLRSKSSNSSDISNNLDTKENTNETNQTKTIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQIDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQ + +    K   SWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK
Sbjct:    1 MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCLNRRRLLRKVLRSKSSNSSDISNNLDTKENTNETNQTKTIPNRVQESEIHLASKKDGDSTLKQLNEKRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQTLNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIKKNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFPKNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETSINNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIPAKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEEVMPNLNRGASNEKNLTVPEGRSRKISQQIDPSNKIDPKVGKITKLALKDLLLIWIEKNTQEYQLISMILTFKTYLSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETAK 517

HSP 2 Score: 112.079 bits (279), Expect = 1.634e-25
Identity = 50/53 (94.34%), Postives = 52/53 (98.11%), Query Frame = 3 
Query: 1887 SCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS 2045
            + +NYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS
Sbjct:  515 TAKNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAKRAENS 567
[Close]
BLAST of Cytospin-A vs. Planmine SMEST
Match: SMESG000039310.1 (SMESG000039310.1)

HSP 1 Score: 80.4925 bits (197), Expect = 9.766e-24
Identity = 39/75 (52.00%), Postives = 55/75 (73.33%), Query Frame = 1 
Query: 1648 KDLLLIWIEKNTQEYQ--NVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA-KFIFIY 1863
            KD++L++ + NT+EY+   V + N S SW+DGLAFCALIHHF+PE+FD+S L+  +   NF LAF +A K+  IY
Sbjct:  341 KDIILMFCKLNTEEYEPYGVCVDNLSSSWSDGLAFCALIHHFHPEAFDFSKLNKLNRKFNFELAFTSADKYADIY 415

HSP 2 Score: 49.6766 bits (117), Expect = 9.766e-24
Identity = 20/48 (41.67%), Postives = 30/48 (62.50%), Query Frame = 3 
Query: 1887 SCRNYAGIEPLIEPGDVNILGSPDEKCVYTYIQFMYNSLKSHPRNSAK 2030
            S   YA I PL++  D+ I+  PD KC++TY+Q  Y + ++HP N  K
Sbjct:  407 SADKYADIYPLLDIDDMMIMEKPDWKCIFTYVQCFYKAFRTHPSNRLK 454
[Close]
BLAST of Cytospin-A vs. Planmine SMEST
Match: SMESG000025457.1 (SMESG000025457.1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.930e-21
Identity = 37/68 (54.41%), Postives = 52/68 (76.47%), Query Frame = 1 
Query: 1642 ALKDLLLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSNDPLKNFTLAFETA 1845
            ++K LLL W  + T++Y  V+I ++S SWA+G+AFCALIHHF+PE+FD++ L   +  KN TLAFETA
Sbjct:  216 SIKQLLLEWCIRQTKDYPGVEIKDFSGSWANGMAFCALIHHFFPEAFDFNQLKPGERSKNLTLAFETA 283

HSP 2 Score: 30.0314 bits (66), Expect = 5.930e-21
Identity = 14/41 (34.15%), Postives = 22/41 (53.66%), Query Frame = 3 
Query: 1887 SCRNYAGIEPLIEPGDVNILG-SPDEKCVYTYIQFMYNSLK 2006
            +    A I PL++  D+ ++   PD KCV+ YIQ  Y   +
Sbjct:  282 TAEKLADISPLLDVKDMLMMKDRPDWKCVFCYIQCFYKRFR 322
The following BLAST results are available for this feature:
BLAST of Cytospin-A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SMTN6.319e-2545.83smoothelin [Source:HGNC Symbol;Acc:HGNC:11126][more]
SMTN4.060e-2447.83smoothelin [Source:HGNC Symbol;Acc:HGNC:11126][more]
SMTNL29.576e-2246.67smoothelin like 2 [Source:HGNC Symbol;Acc:HGNC:247... [more]
SMTN1.869e-2147.83smoothelin [Source:HGNC Symbol;Acc:HGNC:11126][more]
SMTNL22.056e-2146.67smoothelin like 2 [Source:HGNC Symbol;Acc:HGNC:247... [more]
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BLAST of Cytospin-A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
T15B12.13.055e-2355.07pep chromosome:WBcel235:III:5288180:5298361:1 gene... [more]
T15B12.15.654e-2355.07pep chromosome:WBcel235:III:5294501:5298361:1 gene... [more]
T15B12.15.654e-2355.07pep chromosome:WBcel235:III:5294620:5298361:1 gene... [more]
atn-11.158e-1646.58AcTiniN [Source:UniProtKB/TrEMBL;Acc:Q9XVU8][more]
atn-11.430e-1646.58AcTiniN [Source:UniProtKB/TrEMBL;Acc:Q9XVU8][more]
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BLAST of Cytospin-A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG344175.517e-2558.21gene:FBgn0085446 transcript:FBtr0336674[more]
CG344175.524e-2558.21gene:FBgn0085446 transcript:FBtr0345070[more]
CG344175.906e-2558.21gene:FBgn0085446 transcript:FBtr0336670[more]
CG344176.138e-2558.21gene:FBgn0085446 transcript:FBtr0301323[more]
CG344176.246e-2558.21gene:FBgn0085446 transcript:FBtr0303205[more]
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BLAST of Cytospin-A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
smtnb1.900e-2452.17smoothelin b [Source:ZFIN;Acc:ZDB-GENE-070912-2][more]
smtnl15.014e-2248.72smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306... [more]
smtnl16.478e-2248.72smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306... [more]
smtnl7.357e-2240.00smoothelin, like [Source:ZFIN;Acc:ZDB-GENE-040212-... [more]
smtnl1.467e-2139.05smoothelin, like [Source:ZFIN;Acc:ZDB-GENE-040212-... [more]
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BLAST of Cytospin-A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
smtn4.999e-2547.22ES1 protein homolog, mitochondrial-like [Source:Xe... [more]
smtn5.614e-2547.22ES1 protein homolog, mitochondrial-like [Source:Xe... [more]
smtn6.196e-2547.22ES1 protein homolog, mitochondrial-like [Source:Xe... [more]
smtn7.131e-2547.22ES1 protein homolog, mitochondrial-like [Source:Xe... [more]
smtn5.775e-2149.28ES1 protein homolog, mitochondrial-like [Source:Xe... [more]
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BLAST of Cytospin-A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Smtn3.889e-2447.83smoothelin [Source:MGI Symbol;Acc:MGI:1354727][more]
Smtnl22.745e-2246.67smoothelin-like 2 [Source:MGI Symbol;Acc:MGI:24427... [more]
Smtnl23.050e-2246.67smoothelin-like 2 [Source:MGI Symbol;Acc:MGI:24427... [more]
Smtnl13.151e-2252.78smoothelin-like 1 [Source:MGI Symbol;Acc:MGI:19159... [more]
Smtn1.911e-2147.83smoothelin [Source:MGI Symbol;Acc:MGI:1354727][more]
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BLAST of Cytospin-A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8CI12|SMTL2_MOUSE1.855e-2146.67Smoothelin-like protein 2 OS=Mus musculus OX=10090... [more]
sp|Q99LM3|SMTL1_MOUSE1.917e-2152.78Smoothelin-like protein 1 OS=Mus musculus OX=10090... [more]
sp|Q2TAL5|SMTL2_HUMAN4.218e-2146.67Smoothelin-like protein 2 OS=Homo sapiens OX=9606 ... [more]
sp|Q2KI85|SMTL2_BOVIN4.580e-2145.33Smoothelin-like protein 2 OS=Bos taurus OX=9913 GN... [more]
sp|Q2KN97|CYTSA_CHICK1.109e-1931.16Cytospin-A OS=Gallus gallus OX=9031 GN=SPECC1L PE=... [more]
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BLAST of Cytospin-A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1ES987.429e-2463.24Calponin-homology (CH) domain-containing protein O... [more]
A0A226E3T51.411e-2359.09Smoothelin OS=Folsomia candida OX=158441 GN=Fcan01... [more]
A0A4W5RYX61.855e-2344.09Calponin-homology (CH) domain-containing protein O... [more]
A0A1S3PGI12.266e-2344.09smoothelin-like isoform X6 OS=Salmo salar OX=8030 ... [more]
A0A1S3PGP72.798e-2344.09smoothelin-like isoform X4 OS=Salmo salar OX=8030 ... [more]
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BLAST of Cytospin-A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
smtnl4.962e-2352.17smoothelin like 2 [Source:NCBI gene;Acc:103036153][more]
ENSAMXT00000056105.11.320e-2151.47pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
smtnl11.225e-2047.44smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306... [more]
smtnl11.431e-2047.44smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306... [more]
smtnl11.568e-2047.44smoothelin-like 1 [Source:ZFIN;Acc:ZDB-GENE-050306... [more]
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BLAST of Cytospin-A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000008014.13.363e-1949.23pep scaffold:Pmarinus_7.0:GL480769:7574:15080:-1 g... [more]
ENSPMAT00000009649.12.752e-1855.77pep scaffold:Pmarinus_7.0:GL478928:16819:21705:-1 ... [more]
ENSPMAT00000000684.11.069e-1446.27pep scaffold:Pmarinus_7.0:GL483163:49540:93186:-1 ... [more]
ENSPMAT00000009295.11.337e-1446.27pep scaffold:Pmarinus_7.0:GL479988:2885:20778:1 ge... [more]
ENSPMAT00000004049.12.087e-1244.78pep scaffold:Pmarinus_7.0:GL476488:489896:554954:1... [more]
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BLAST of Cytospin-A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytospin-A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO368783.084e-1646.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO368776.997e-1646.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
ALPHA-ACTININ1.121e-1547.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO361242.566e-1447.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377084.189e-1441.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cytospin-A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000042079.12.617e-2339.78smoothelin [Source:NCBI gene;Acc:101173151][more]
smtna9.537e-2351.47smoothelin-like protein 1 [Source:NCBI gene;Acc:10... [more]
smtnl15.122e-2250.00smoothelin like 1 [Source:NCBI gene;Acc:101166638][more]
smtnl15.122e-2250.00smoothelin like 1 [Source:NCBI gene;Acc:101166638][more]
smtnl3.028e-2150.00smoothelin like 2 [Source:NCBI gene;Acc:101158374][more]
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BLAST of Cytospin-A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000002849.11.455e-25100.00SMESG000002849.1[more]
SMESG000002849.11.657e-2593.28SMESG000002849.1[more]
SMESG000002849.11.634e-2597.87SMESG000002849.1[more]
SMESG000039310.19.766e-2452.00SMESG000039310.1[more]
SMESG000025457.15.930e-2154.41SMESG000025457.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016941 ID=SMED30016941|Name=Cytospin-A|organism=Schmidtea mediterranea sexual|type=transcript|length=2284bp
CAGCTGAAGTCGCTATACAATCGCTTCCGAAAACTCTGTACAACTTATGG
AGAAGAGTTAATACGAGTAAACAACATTAAACATTCAGAACAAATATCAT
GGACAGATTGATGGCCATAAATATTCAACAATGACCTTCAATTGATGAGC
AGATCAAGTGTGTTATTAAATCATCATTATCATCAACATCATCATCATCT
CTTATTCTATCAACAAATTACTGATCTGATCAATTCAATTTTATTCTACT
AAAATTTGCCATCAGTAGCACTTTCTCTATTTTTGGATTTTCATACCGAA
CCGAATATGTCGAATTTTCTTGATAATAAAACGGATGCTGAAATCGAGGA
AATATACAACGGTGCAACGATGGAGGATCTTCAATCTATGCTAACTCAAG
AAAAATGTCTCAACAGAAGAAGATTATTACGAAAGGTCTTGAGAAGTAAA
TCAAGCAATTCTTCAGATATCTCAAATAACCTAGACACCAAAGAAAACAC
GAACGAAACAAATCAAACTAAAACTATTCCGAATAGAGTCCAGGAAAGTG
AAATTCACTTAGCATCAAAAAAGGATGGCGATAGTACATTAAAACAACTT
AATGAGAAGAGAAAACAACTTCACGATGCTGCTGACTTAGCGCAAAGAAA
GGAGTTGCGAAAAGAAATCCAAATTTTAGAAAATCAATGCAACAAACAAC
CGAATACGATAAATCAGAATGCCAGAACTCTTTTGAATACAGAATCAAAT
CAAACTTTAAATAGTTGTAAGGAAAAACCAGAAGCAGAGGATATGCATTC
TTCTTCAAAGAATTTTCATACCGACACAAAAGATCAAGTTACCTCGGATA
ACAAGCCCAAGTTTAATTTCATTAAAACTCCAGAAAAAGATCCTCATCCA
ATTAAGAAAAACGAGAATGAATTTCGAAATGTCAGCAACAAACCTTCGGA
TGATAAAAATAATAAAATGTCCGCTGCATGTCTAGAAAATTCAACAAAAG
TCAAAAATAACCAAACTGACTGTGTAGATACGAAAAACTTGACAGAAGAT
TTTCCAAAAAACCAAAAACCGATTTCTGAGAGAAGCAAAATTTCTATCAT
AGATAAATTAGCTGGAAAAGATCAGAAACCAACAGATAAAAGAAATTCCA
TATCTACTCCGGATGGAAACACAAGCAAATATCCACCACCGCCTAAAGAA
ACTTCAATCAACAACTCCTTTCACAATTCACAGAAGTTCGAACCCCAATC
TCGTTCTGTTAACCCGTCTACTGATTCAATAATCAAACAAATACCATTGA
AGGATGAAATGTCCGCTTCAAAACCTATTCCACAAATGTCAACTCCAGAA
ATACCGGCTAAGCAAAATATTTCTCGAGTTAGCAGTGTCAGAGAAAAATT
TGAAAACCCCCCAGCTCCCAGTTTTACAAACAAAGCTAAAACTTCAACTA
AATTTAGCGAAAAAGAATTAAATGACATTCTAAACGCCGTTTTAAAACCA
GAGGAAGTAATGCCCAATTTAAATAGAGGAGCTTCGAATGAGAAAAATCT
TACAGTTCCAGAAGGCCGGTCCCGAAAGATTAGTCAACAGATAGATCCAA
GCAACAAAATAGATCCTAAAGTCGGCAAAATTACGAAATTAGCTTTGAAA
GACCTTCTCTTAATTTGGATTGAAAAAAACACTCAAGAATATCAAAATGT
AAAAATTACCAATTATTCAAAAAGTTGGGCTGATGGATTAGCATTTTGCG
CCTTGATTCATCATTTTTATCCTGAGTCGTTTGATTACAGCGTCCTTTCA
TCAAATGACCCTTTGAAAAACTTTACTCTGGCTTTTGAAACTGCTAAGTT
TATTTTTATATATTATTCAGTAAAATATTTATAATTTCATGCAGGAATTA
TGCTGGTATCGAGCCTTTGATTGAGCCAGGAGATGTGAATATCCTTGGGT
CACCGGATGAAAAATGCGTCTATACCTACATCCAATTTATGTACAATTCT
CTGAAAAGTCATCCAAGAAATTCGGCAAAACGAGCAGAAAATTCTTGAAA
CTATGTTATTTGAGATTATTTAGACTAGAAATTGGCAACTATATGTATCA
AATAGATATCATTTGTTCATTCTTCGGTTTGAATTTATTAGAAATAGAAG
GACAAAAAAGAGAATCAACTGATCGAATAATTTCCAAAGGAAATTCATAT
AGTATTGTTGGTTTACAAATAGTTTAGTAGAAATTGTATCTTGACTGTAT
CCACGCATTCACAGTTTGATAATTAAGAACCGAC
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protein sequence of SMED30016941-orf-1

>SMED30016941-orf-1 ID=SMED30016941-orf-1|Name=SMED30016941-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=526bp
MSNFLDNKTDAEIEEIYNGATMEDLQSMLTQEKCLNRRRLLRKVLRSKSS
NSSDISNNLDTKENTNETNQTKTIPNRVQESEIHLASKKDGDSTLKQLNE
KRKQLHDAADLAQRKELRKEIQILENQCNKQPNTINQNARTLLNTESNQT
LNSCKEKPEAEDMHSSSKNFHTDTKDQVTSDNKPKFNFIKTPEKDPHPIK
KNENEFRNVSNKPSDDKNNKMSAACLENSTKVKNNQTDCVDTKNLTEDFP
KNQKPISERSKISIIDKLAGKDQKPTDKRNSISTPDGNTSKYPPPPKETS
INNSFHNSQKFEPQSRSVNPSTDSIIKQIPLKDEMSASKPIPQMSTPEIP
AKQNISRVSSVREKFENPPAPSFTNKAKTSTKFSEKELNDILNAVLKPEE
VMPNLNRGASNEKNLTVPEGRSRKISQQIDPSNKIDPKVGKITKLALKDL
LLIWIEKNTQEYQNVKITNYSKSWADGLAFCALIHHFYPESFDYSVLSSN
DPLKNFTLAFETAKFIFIYYSVKYL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005819spindle
GO:0005921gap junction
GO:0001726ruffle
GO:0005634nucleus
GO:0005737cytoplasm
GO:0005829cytosol
GO:0005856cytoskeleton
GO:0005886plasma membrane
GO:0016020membrane
GO:0030018Z disc
GO:0030054cell junction
GO:0042995cell projection
GO:0043197dendritic spine
Vocabulary: biological process
TermDefinition
GO:0007049cell cycle
GO:0051301cell division
GO:0051017actin filament bundle assembly
GO:0051764actin crosslink formation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036872CH_dom_sf
IPR001715CH-domain
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003779actin binding
GO:0005509calcium ion binding
GO:0019904protein domain specific binding
GO:0046872metal ion binding
GO:0051015actin filament binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 95..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..219
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..214
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 156..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 362..381
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..279
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..79
NoneNo IPR availablePANTHERPTHR23167FAMILY NOT NAMEDcoord: 409..513
IPR001715Calponin homology domainSMARTSM00033ch_5coord: 447..525
e-value: 3.7E-4
score: 29.8
IPR001715Calponin homology domainPFAMPF00307CHcoord: 447..514
e-value: 5.4E-14
score: 52.3
IPR001715Calponin homology domainPROSITEPS50021CHcoord: 445..525
score: 18.131
IPR001715Calponin homology domainCDDcd00014CHcoord: 448..516
e-value: 1.84695E-14
score: 67.3359
IPR036872CH domain superfamilyGENE3DG3DSA:1.10.418.10coord: 443..517
e-value: 5.7E-22
score: 79.6
IPR036872CH domain superfamilySUPERFAMILYSSF47576Calponin-homology domain, CH-domaincoord: 445..518