SMED30016927

Overview
NameSMED30016927
Smed IDSMED30016927
Length (bp)4523
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016927 (SMED30016927) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016927 (SMED30016927) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016927 (SMED30016927) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016927 (SMED30016927) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016927

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30016927 dd_Smed_v4_17002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016927 dd_Smed_v4_17002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016927 dd_Smed_v4_17002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016927 dd_Smed_v4_17002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30016927 vs. TrEMBL
Match: H9JJ88 (BED-type domain-containing protein OS=Bombyx mori OX=7091 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.409e-44
Identity = 57/189 (30.16%), Postives = 94/189 (49.74%), Query Frame = -1 
Query: 1887 LGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKKVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEMEQNSECFLLVSDIKSALYAMRKVIKFFKNSSIRNEILLKHVRQKEGKNLKLILDVKTRWNTLASAVNRF 2453
            LGL++I   C  E+  ELV+  L+ F ++L+TDIV  T D ASVM K   +++  +QLC+ H I LAV+  LY++                 +           ++    E+  +     EQ      L+ + +  +  +RKV++ F+ S+ +   L K+ R+  GK   LILD +T+W+TL   + RF
Sbjct:  207 LGLIRIHGSCNVEQCVELVKTRLEAFILHLDTDIVGITTDDASVMKKVGRLLSCYQQLCFAHGIQLAVVNTLYRE------------EVPHTQHKDKLLAACYSDDDDSSEEPDSSYTHFEQPPA--ELIPNYRGLIDKVRKVVRLFRKSATKEACLQKYAREDLGKEYGLILDCRTKWSTLYDMLERF 381

HSP 2 Score: 80.1073 bits (196), Expect = 2.409e-44
Identity = 60/191 (31.41%), Postives = 92/191 (48.17%), Query Frame = -3 
Query: 2485 SKRKSEAWNYFQKVNANEAKCNHCGTILSCKGSSTTGLINHLKLHDLLISRNSKCNDTDSEPSTSK--TMKLSNS---------------SIKSFLKSQISLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYSMPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNV 3006
             KRK + W+YF K   N AKC+ C  +L     STTGL  HLK    +   NS+  + +  PST +  ++K  N                ++ + L ++ S   ++++  AKDG +FN +  S+ +K   T    S+P    +I+  V NF    K E    L  L    +KFS+  DEWT +  +R+LNV
Sbjct:    6 GKRKKQIWDYFIKTGQN-AKCSLCEHVLKISQRSTTGLKYHLKSKHFVDLANSQAAEQEPGPSTPQDASLKTDNQLYDDDEPRNKVPKKYTLDNCLTNEHSREVMISRMVAKDGLTFNCLSASEDLKYLFTKNGLSLPTCPDAIKTIVENFSMTVKAEMIKTLQNLKIRGEKFSLGFDEWTTITCKRYLNV 195

HSP 3 Score: 65.0846 bits (157), Expect = 2.409e-44
Identity = 62/228 (27.19%), Postives = 114/228 (50.00%), Query Frame = -3 
Query: 1123 AVQELSKKDANLITAEGTILYVMKKLKNIDTELSLNLLEILKNEIGKRRNRDMVSLLIFLHQGT*----VKQNENEFLNYSSKSSLKNYAK*LFLRLFPHNEFENR-------EAEEDLETEIIHLEEDELTEYENLQNCIQSLARPKQVKXXXXXXXXXXXNLQGNKGKRSDDMDCLYNAL-TVAPTSTSCERVFSVSGNKMTKIRSRLGVNTLNALVFLKYYFNQN 1770
             V+ L ++D+ L+TA+ T+ ++++KL    + +S +L   L+  I +RR  D+  ++++LH        VK  E+ FL    K+ ++     +  RL    E   R       + E++   E I L ++  TE    +N   ++  P + +  E  I+ E    + NKG +   +  ++  L T++PTS   ER FSV+G   + IR+++   TL+ + FL+ YF  N
Sbjct:  422 GVEVLCRRDSTLLTADTTLRFILEKLDKQQSRVSGDLATALRMRIKERRT-DLSGIVLYLHNPKKYEKSVKSPEDTFL-LPKKNRIRLELIKICERLLTVKEDTGRAEGEHFDDEEDNSSDEDITLRDELETELRKQKNETCTVIAPIKSQNFEKTIKAEMVEYE-NKGIKGKHLTMVHKYLNTISPTSVEAERAFSVTGYVCSPIRNKMTDRTLDTICFLRSYFQNN 646
[Close]
BLAST of SMED30016927 vs. TrEMBL
Match: A0A0J7KNG8 (Dimer_Tnp_hAT domain-containing protein OS=Lasius niger OX=67767 GN=RF55_8155 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.272e-41
Identity = 65/190 (34.21%), Postives = 115/190 (60.53%), Query Frame = -1 
Query: 1887 LGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKKVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEMEQNSECFLLVSDIKSALYAMRKVIKFFKNSSIRNEILL-KHVRQKEGKNLKLILDVKTRWNTLASAVNRF 2453
            LGL++I+   +AE    LVE  LK F +N++ DIVA T DG +VM+K  +++   +Q C+ H I LA++ ILYK +      E +D+      + + + D  +  +  D ++EG+ +++   +S    + S+ K  +  +RKV+K F+ S  +N+++L K+++++  K L+LILD KTRWN+L + + RF
Sbjct:  101 LGLIRIQGSSSAEHCVNLVEEHLKIFNINIKDDIVAITADGPNVMIKVGNLMPCFQQRCFAHGIQLAIIDILYKDL---PEEEAQDQEGLRRFQEIDNADQSELSDVDDDDNEGSFIVDCIPSS--VKIASNYKDLITKVRKVVKIFRRSPTKNDLILQKYIQEEHDKRLELILDCKTRWNSLVNMLERF 285

HSP 2 Score: 70.4774 bits (171), Expect = 1.272e-41
Identity = 77/274 (28.10%), Postives = 128/274 (46.72%), Query Frame = -3 
Query: 1123 CGNNALEELGFQ-KFDEKYILVLKSISITLEPTMLAVQELSKKDANLITAEGTILYVMKKLKNIDTELSLNLLEILKNEIGKRRNRDMVSLLIFLHQG----T*VKQNENEFLNYSSKSSLKNYAK*LFLRLFPHNEFE--------------NREAEEDLETEIIHLEED-EL---TEYENLQNCIQSLARPKQVKXXXXXXXXXXXNLQGNKGKRSDDMDCLYNAL-TVAPTSTSCERVFSVSGNKMTKIRSRLGVNTLNALVFLKYYFNQN 1872
            C + AL +L  + +F E     +  +  +L+P  L V+ L ++DA LITAE T+ ++++KL N  T LS  L   L+  I +RR  D+   L++L         +K  ++ FL    K+ ++   K L  R+  +   E              + E +E  E+  + L+E+ E+    EY  ++  I+S    K ++       I F     N G +   +   Y  L T+ PTS   ER FS +G     +R+ LG  ++N+L FL+ YF  N
Sbjct:  291 CISKALIDLRSEIRFTETEWFAINDLKNSLQPVKLGVKVLCRRDATLITAETTVRFILEKLDNQSTPLSTKLAAALRRRIKQRR-IDLTGTLLYLQNPIKFENDLKNPDDTFL-LPKKTIIRKEIKKLLERVLSNEHGEKILCASNTASCSNNSDEDQEQDESRTLSLKEELEMKLQKEYPEVK-VIESKVLSKNLENVLKKEMIAFE----NDGTKDRYLRMAYEYLLTIPPTSVEAERAFSAAGYIRNNLRTSLGDESINSLCFLRSYFQNN 557

HSP 3 Score: 55.0694 bits (131), Expect = 1.272e-41
Identity = 29/87 (33.33%), Postives = 47/87 (54.02%), Query Frame = -3 
Query: 2485 ILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYSMPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNV 2745
            ++++  AKDGFSFN+   S  ++       + +P S  +I+  V+ F E  K     E+ +L    +KFS+  DEWT    RR+LN+
Sbjct:    4 MISRMTAKDGFSFNSFCKSSDLRYLFQKSGHKLPSSSNTIRSIVIQFSESVKANMINEIQKLQMQNQKFSLTFDEWTSRKNRRYLNI 90
[Close]
BLAST of SMED30016927 vs. TrEMBL
Match: A0A2A4J235 (BED-type domain-containing protein OS=Heliothis virescens OX=7102 GN=B5V51_8278 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.581e-41
Identity = 61/202 (30.20%), Postives = 101/202 (50.00%), Query Frame = -1 
Query: 1887 LGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKK-----VNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEMEQNSECFLLVSDIKSALYA-------MRKVIKFFKNSSIRNE-ILLKHVRQKEGKNLKLILDVKTRWNTLASAVNRF 2453
            LGL++I     A+K  E ++  L EFG++LE DIVA T DG S+M K+   +++  Q C  HA+ LA+  + YKK      N  + +E++D  +     L+      +  N     D  +     E +S      S++    +        ++KV+K F+ S  +N+ IL K+V  + G+   L  D  TRW++LA+ + RF
Sbjct:  210 LGLIRIYGSLPAKKCVEEIKRKLDEFGVSLEKDIVAETTDGCSMMTKFGKFLSSYHQKCIAHALQLAIADVFYKKSEALIANAAEELEEDDDDDLPLSVLVRQAHQSNTHNSASDNDSDDDEDGAEFSSNAVTTTSEVSEQQFVYHDLIAKVKKVVKIFRKSPTKNDNILQKYVVDEFGREYSLTCDAITRWSSLANTLERF 411

HSP 2 Score: 74.7146 bits (182), Expect = 1.581e-41
Identity = 88/284 (30.99%), Postives = 132/284 (46.48%), Query Frame = -3 
Query: 1126 LKLIECGNNALEELGFQK-FDEKYILVLKSISITLEPTMLAVQELSKKDANLITAEGTILYVMKKLKNIDTELSLNLLEILKNEIGKRRNRDMVSLLIFLHQGT*VKQNENE-FLNYSSKSSLKNYAK*LFLRLFPH---NEFEN-------REAEEDLET----EIIHLEEDE--------LTEYENLQNCIQSLARPKQVKXXXXXXXXXXXNLQ-----GNKGKR-SDDMDCLYNALTVAPTSTSCERVFSVSGNKMTKIRSRLGVNTLNALVFLKYYFNQ 1887
            LKL  C   AL +L  Q  F +     L+     L+     ++ L ++DA L+TA+  I + + KL NIDT LS  ++  LK    +RR  +  S+L +L       QN +  F N S +           L +FP    NE  N       R +   L T    +II +EED         LT+ E L   ++ + R + V +N+         +      G++G+  S   D L   LT+ PTS   ER+FS +G    KIRSRLG  TL+AL+FL+ ++N+
Sbjct:  412 LKLKNCIVKALIDLKVQIIFSDSDWKDLEQFHEILDIVKTVLEALCRRDATLLTADVAIKFALNKLSNIDTHLSRKMISALKTRYTQRRLIE-ASILNYL-------QNPDTYFANVSDED----------LSVFPRPNSNEMCNKIVLLLKRTSPPLLTTIQPNQIIEIEEDSELSAERSNLTKGEELNAELKKICRERVVTQNDDIETNVRIEMALFENGGDRGRHLSKAFDHL---LTIPPTSVEPERIFSSAGLICNKIRSRLGDVTLDALIFLRTHYNE 674

HSP 3 Score: 69.3218 bits (168), Expect = 1.581e-41
Identity = 51/182 (28.02%), Postives = 90/182 (49.45%), Query Frame = -3 
Query: 2485 KSEAWNYFQKV-NANEAKCNHCGTILSCKGSSTTGLINHLK-LHDLLISRNSKCNDTDSE------PST-SKTMKLSNSSIKSFLKSQI--SLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYSMPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNV 2997
            K+  W+YF +  +   AKC  C  IL C G ST+GL  HLK +H +   +     ++  E      PST S+ +    + +  + +S    S+  ++A+  + +G+SF   I  + ++       + +P    +I+  V+   ++ K+  K E+  LL    KFSI  DEWT +  RR+L++
Sbjct:   13 KTSVWHYFLRARDKVSAKCKKCQKILKCVGGSTSGLQKHLKNVHQITGEKQVSITESHGEVSNMVIPSTSSEDIPKKKTKVTDYYESDTNPSMQLMIARMTSMNGYSFRTFIECEDLRTLFLKSGHKLPLCADTIRNIVVKEADKIKRLLKAEIQNLLKEHHKFSISFDEWTSIRNRRYLSL 194
[Close]
BLAST of SMED30016927 vs. TrEMBL
Match: A0A226DDR7 (Putative AC9 transposase OS=Folsomia candida OX=158441 GN=Fcan01_16136 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 3.026e-41
Identity = 65/192 (33.85%), Postives = 98/192 (51.04%), Query Frame = -1 
Query: 1887 YFLGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYC-SIITTKKQLCYNHAIHLAVLKILYKKVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEMEQNSECFLLVSDIKSALYAMRKVIKFFKNSSIRNEILLKHVRQKEGKNLKLILDVKTRWNTLASAVNRF 2459
            + LGLV I    TAE   +LV+  +   G+NL  DIVA   DG+SVM K       T +QLC  H I LAVL +LY K   +    D  +   ++     D+  +  E     ++      ++ + S+   L   +K  +  +R  +K F+ S  +NEIL  +V+ +  K L LILD +TRWN+LA+ + RF
Sbjct:  206 WNLGLVPILGSATAENCLQLVDEKITSVGLNLHKDIVAIITDGSSVMKKIGRDAAPTFQQLCLAHGIQLAVLDVLYAK-GENVGDTDNLEAIDENSSFSDDEGSEFVEYASPDDENAETDFDLTEESDAPSLNPILKPIIDKIRSTVKMFRRSPKKNEILQNYVKSEFQKELNLILDCRTRWNSLAAMIERF 396

HSP 2 Score: 71.2478 bits (173), Expect = 3.026e-41
Identity = 63/232 (27.16%), Postives = 113/232 (48.71%), Query Frame = -3 
Query: 1120 TLEPTMLAVQELSKKDANLITAEGTILYVMKKLKNIDTELSLNLLEILKNEIGKRRNRDMVSLLIFLHQGT*VKQNENEFLNYSSKSSLKNYAK*LFLR-----LFPHNEFENREAEED--LETEIIHLEEDELTEYENLQNCIQSLARPKQVKXXXXXXXXXXXNLQGNKGKRSDDMDCLYN-ALTVAPTSTSCERVFSVSGNKMTKIRSRLGVNTLNALVFLKYYFNQNV 1791
             L+P   AV+ +  +++NLIT +  +  V+ KL+   + +SL L   +K  + +RR  ++  +L +LH      ++E E  +  +++ ++N+ K +  R     +    E  +  A ED  ++       +DEL E         +  + K+ +     +  E    + + G R  ++  +Y+  LTV PTS   ER FSVSG    K RSRL  +TL+ L +LK YF ++ 
Sbjct:  430 ALKPIRAAVEVICNRESNLITTDAALNLVISKLRAQKSSVSLELASSIKFRVMERRT-ELTKVLRYLHNRVYANEDE-ELADTITRTEVQNFLKKMLTRTRIGTVVGDEESNDTPANEDFTVQPSAAATFKDELNEIMKKAMTPPTTEKSKKSQTLLTTLRQEMKLFE-DGGTRGYNLSLIYDYLLTVKPTSVESERAFSVSGYVCNKFRSRLSDSTLDDLCYLKSYFKRSA 658

HSP 3 Score: 60.8474 bits (146), Expect = 3.026e-41
Identity = 60/190 (31.58%), Postives = 86/190 (45.26%), Query Frame = -3 
Query: 2452 WNYFQKVNANE-AKCN--HCG-TILSCKGSSTTGLINHLK-LHDL-LISRNSKCNDTDSEPSTSKTMKLSNSSIKSFLKSQISLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYS-MPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNVTAL-----FKFIGIFP 2985
            W+YF K +    AKC    C   IL   G ST G  +HLK +H++ L   N    D DS        K S            +L    A+ AA DG SF  M +S  I+  + S  +S +  S  +I+  V  F +  +   + E+++L     KFSI  DEWT +  RR+L+V  L     F  +G+ P
Sbjct:   23 WHYFLKESTGATAKCKDPECKKPILKTSGGSTKGCRDHLKRIHNVDLDIINLTSVDLDSGRQREHASKKSKRITDYLSVKHDTLQIAYARMAALDGLSFQVMANSYDIRAGLKSRGFSEVLSSPNAIKSKVRKFAQSLRDNDRKEIMQLNEQGTKFSITFDEWTSMRNRRYLSVILLGKQQQFWNLGLVP 212
[Close]
BLAST of SMED30016927 vs. TrEMBL
Match: A0A226D4I7 (Putative AC transposase OS=Folsomia candida OX=158441 GN=Fcan01_25450 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 7.779e-41
Identity = 64/191 (33.51%), Postives = 102/191 (53.40%), Query Frame = -1 
Query: 1887 YFLGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKKVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEMEQNSECFLLVSDIKSALYAMRKVIKFFKNSSIRNEILLKHVRQKEGKNLKLILDVKTRWNTLASAVNRF 2459
            + LGL++I     AEK  E+V N L+   ++LE DIV    DG+SVM K   +  T +QLC+ H I L VL +LYKK+N     ++ +    D+     D    D ++              E   E  ++   ++  +  +R +++ F+ S ++NEIL  +++Q+ GK L LILD KTRWN+L + + RF
Sbjct:  213 WNLGLIRIHGSLPAEKCLEMVANKLRLLNLSLENDIVCIITDGSSVMTKIGRLSPTYQQLCFAHGIQLGVLDVLYKKINQTPEADESENENLDNLAPCEDDSCCDSDSDS--------EEIDEDEDEDLVISHTLRPVIDKVRTIVRMFRRSPLKNEILQTYIKQEFGKELSLILDCKTRWNSLVAMLERF 395

HSP 2 Score: 74.7146 bits (182), Expect = 7.779e-41
Identity = 58/180 (32.22%), Postives = 87/180 (48.33%), Query Frame = -3 
Query: 2485 KSEAWNYFQK--VNANEAKC--NHCGTILSCKGSSTTGLINHLK-LH--DLLISRNSKCNDTDSEPSTSKTMKLSNSSIKSFLKSQI-SLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYS-MPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNV 2997
            K   WNYF K   +   AKC    C  +L   G ST+GL  HL+ +H  DLL          D  PS       S   I  F + +  SL E LA+  AKDG  F+    S  ++  + S  ++ +PKS  +I+K V+ + ++ K      ++ L    +KFS+  DEW+ +  RR++NV
Sbjct:   32 KDSIWNYFLKEVADGKTAKCMDTSCQKVLKTAGGSTSGLNTHLRSIHHIDLL--------KKDLNPSELGVKAKSVKPISDFFEKKTKSLEETLARMTAKDGIPFSVFSTSTDLRRALKSDGFTDLPKSAHTIKKIVIGYSQKIKSTENEHVLDLKRIGRKFSVTFDEWSSIKNRRYMNV 203

HSP 3 Score: 43.8986 bits (102), Expect = 7.779e-41
Identity = 32/120 (26.67%), Postives = 63/120 (52.50%), Query Frame = -3 
Query: 1429 LEPTMLAVQELSKKDANLITAEGTILYVMKKLKNIDTELSLNLLEILKNEIGKRRNRDMVSLLIFLHQGT*VKQNENEFLNYSSKSSLKNYAK*LFLRLFPHNEFENREAEEDLETEIIH 1788
            L+P    ++ L ++DANL+TA+  + ++++KL    + L+L L   L+  I +RR  ++  L  +LH G  +   ++E  +    + + +  + +  RL   N+ +  E +E  ET II 
Sbjct:  430 LKPIEATIEALCRRDANLLTADTALNFMIQKLDMQQSGLALELGVALRTRIEERRT-ELSGLFKYLHTGQYINSEQDEIFSIPKPTKICSLIRNIICRLRGMND-DASELQEG-ETNIIE 546
[Close]
BLAST of SMED30016927 vs. Planmine SMEST
Match: SMESG000022344.1 (SMESG000022344.1)

HSP 1 Score: 109.383 bits (272), Expect = 1.793e-70
Identity = 89/254 (35.04%), Postives = 135/254 (53.15%), Query Frame = -3 
Query: 1132 CGNNALEELGFQK-FDEKYILVLKSISITLEPTMLAVQELSKKDANLITAEGTILYVMKKLKNIDTELSLNLLEILKNEIGKRRNRDMVSLLIFLHQGT*VKQNENEFLNYSSKSSLKNYAK*LFLRLF--PHNEFENREAEEDLETEIIHLEEDELTEYENLQNCIQS-LARPKQVKXXXXXXXXXXXNLQ--GNKGKRSDDMDCLYNAL-TVAPTSTSCERVFSVSGNKMTKIRSRLGVNTLNALVFLKYYF 1872
            C  +ALEE+G    ++EK   +L+++   L+PT  AV+ELSK  + LITAE  + ++  ++K  +T L       LK  I +RRN+++++L  FL  GT   Q +   L YS+K+   + A  L  RLF  P+ +F     EE     I        T  + LQ  I + L   K+   +          L+  G   ++S  +  L+NAL ++ PTST  ERVFS++GN  TK+RS +    L+AL+FLKYYF
Sbjct:  340 CIKHALEEIGCDDLYNEKDFNILENLIEILKPTEFAVKELSKHTSTLITAERVLTFLFTQMKQNNTALGKKFYNSLKIGISERRNKELITLYKFLQSGT-FPQPDLMKLPYSTKAPTLSLATMLAARLFGEPNPQFAEENIEESEVEPI---ASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQKELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFLKYYF 589

HSP 2 Score: 102.834 bits (255), Expect = 1.793e-70
Identity = 73/193 (37.82%), Postives = 107/193 (55.44%), Query Frame = -1 
Query: 1890 YFLGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKKVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEME-QNSECFLLVSD--IKSALYAMRKVIKFFKNSSIRNEILLKHVRQKEGKNLKLILDVKTRWNTLASAVNR 2459
            Y LGL+ I+  C A +   LVE  L  + + L  +I+AST DGASVM KY  +   + Q C NHAIHLAV++  Y K N+ D + D +  + D++E  ++    D           NL ++ +  N+E    + D  I   L   RK ++FF+ S +RN+IL ++   KEGK  KLILD +TRWN+L   + R
Sbjct:  152 YKLGLIAIEGSCDAYQIHSLVEQKLMYYDIKLR-NILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGDH----------NLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYDEDKEGKKFKLILDCRTRWNSLIPMMER 333

HSP 3 Score: 97.4413 bits (241), Expect = 1.793e-70
Identity = 60/153 (39.22%), Postives = 93/153 (60.78%), Query Frame = -3 
Query: 2482 CGTILSCKGSSTTGLINHLKL-HDLLISRNSKCNDTDSEPSTSKTMKLSNSSIKSFLKSQISLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYSMPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNVT 2937
            C ++L C G + + L NHL+  HD+ + +N        EP   +    SN  + +F+K + S+ E+LAK A  DGFS +AM HS AI+  +T+ +Y MPKS ++I+K +++F  EKK + K + I++ +S   FSI  DEWT    +R+LNVT
Sbjct:    2 CNSVLKCAGGNISNLANHLRAKHDIDVKKNE-------EPPEKRAKATSN--MLNFIKRE-SVGELLAKCAVYDGFSIHAMTHSSAIREFITTRSYVMPKSESTIKKLIVDFANEKKLQFKDKFIKMANSGMCFSITTDEWTSCSGKRYLNVT 144
[Close]
BLAST of SMED30016927 vs. Planmine SMEST
Match: SMESG000026901.1 (SMESG000026901.1)

HSP 1 Score: 115.546 bits (288), Expect = 6.388e-67
Identity = 71/177 (40.11%), Postives = 106/177 (59.89%), Query Frame = -3 
Query: 2482 MSKRKSEAWNYFQKVNANEAKCNHCGTILSCKGSSTTGLINHLKL-HDLLISRNSKCNDTDSEPSTSKTMKLSNSSIKSFLKSQISLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYSMPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNVT 3009
            M+ +KSE W  FQK   N AKC  C ++L C G +T+ L NHL+  HD+ + +N        EP   +    SN  + +F+K + S+ E+LAK AA DGFS +AM HS AI+  +T+ +Y MP+S ++I+K +++F  EKK + K +  ++  S    SI  DEWT    +R+LNVT
Sbjct:    1 MNSKKSEVWKIFQKETDNTAKCTMCNSVLKCAGGNTSNLANHLRAKHDIDVKKNE-------EPPEKRAKATSN--MLNFIKRE-SVGELLAKCAAYDGFSIHAMTHSSAIREFITTRSYVMPRSESTIKKLIVDFANEKKIQFKDKFTKMAESGICLSITTDEWTSCSGKRYLNVT 167

HSP 2 Score: 109.383 bits (272), Expect = 6.388e-67
Identity = 75/194 (38.66%), Postives = 111/194 (57.22%), Query Frame = -1 
Query: 1887 YFLGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKKVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEME-QNSECFLLVSD--IKSALYAMRKVIKFFKNSSIRNEILLKHVRQKEGKNLKLILDVKTRWNTLASAVNRF 2459
            Y LGL+ I+  C A +   LVE  L ++G+ L  +I+AST DGASVM KY  +   + Q C NHAIHLAV++  + K N+ D + D +  + D++E  ++    D           NL ++ +  N+E    + D  I   L   RK ++FF+ S +RN+IL ++V  KEGK LKLILD +TRWN+L   + R 
Sbjct:  175 YKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKLR-NILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFHNKNNVIDEISDSENADGDEDENQFNSFAGDH----------NLNIDNDIDNTEYTYSLDDAKINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDSRTRWNSLIPMMERL 357

HSP 3 Score: 72.4034 bits (176), Expect = 6.388e-67
Identity = 50/136 (36.76%), Postives = 79/136 (58.09%), Query Frame = -3 
Query: 1474 CGNNALEELGFQK-FDEKYILVLKSISITLEPTMLAVQELSKKDANLITAEGTILYVMKKLKNIDTELSLNLLEILKNEIGKRRNRDMVSLLIFLHQGT*VKQNENEFLNYSSKSSLKNYAK*LFLRLF--PHNEF 1872
            C  +ALEE+G    ++EK   +L ++   L+PT LAV+ELSK  + L+TAEG + ++  ++K  +T L       LK  I +RRN+++++L  FL  GT  + +  E L YS+K+   + A  L  RLF  P+  F
Sbjct:  363 CIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKIFYNSLKIRISERRNKELITLYKFLQSGTFPQPDLME-LPYSTKAPTLSLATMLAARLFGEPNPPF 497
[Close]
BLAST of SMED30016927 vs. Planmine SMEST
Match: SMESG000008701.1 (SMESG000008701.1)

HSP 1 Score: 216.853 bits (551), Expect = 6.876e-62
Identity = 108/119 (90.76%), Postives = 111/119 (93.28%), Query Frame = -3 
Query: 2656 IMSKRKSEAWNYFQKVNANEAKCNHCGTILSCKGSSTTGLINHLKLHDLLISRNSKCNDTDSEPSTSKTMKLSNSSIKSFLKSQISLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCN 3012
            I+SKRKSEAWNYFQK N NEAKCNHCGTILSCKGSSTTGLINHLKLHDLLISRNSKCNDTDSEPS SKTMK+SNSSIKSFLKSQISLSEILAK AAK GFSFNAMIH DAIKG VT+ N
Sbjct:  178 IISKRKSEAWNYFQKANVNEAKCNHCGTILSCKGSSTTGLINHLKLHDLLISRNSKCNDTDSEPSKSKTMKISNSSIKSFLKSQISLSEILAKCAAKHGFSFNAMIHLDAIKGYVTNHN 296
[Close]
BLAST of SMED30016927 vs. Planmine SMEST
Match: SMESG000077975.1 (SMESG000077975.1)

HSP 1 Score: 116.701 bits (291), Expect = 3.060e-51
Identity = 72/177 (40.68%), Postives = 107/177 (60.45%), Query Frame = -3 
Query: 2482 MSKRKSEAWNYFQKVNANEAKCNHCGTILSCKGSSTTGLINHLKL-HDLLISRNSKCNDTDSEPSTSKTMKLSNSSIKSFLKSQISLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYSMPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNVT 3009
            M+ +KSE W  FQK   N AKC  C +IL C   +T+ L NHL+  HD+ + +N        EP   +    + SS+ +F+K + S+ E+LAK AA DGFS +AM HS AI+  +T+ +Y MP+S ++I+K +++F  EKK + K +  ++  S   FSI  DEWT    +R+LNVT
Sbjct:    1 MNSKKSEVWKIFQKETDNTAKCTMCNSILKCASGNTSNLANHLRAKHDIDVKKNE-------EPPEKRAK--ATSSMLNFIKRE-SVEELLAKCAAYDGFSIDAMTHSSAIREFITTRSYVMPRSESTIKKLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCSGKRYLNVT 167

HSP 2 Score: 107.071 bits (266), Expect = 3.060e-51
Identity = 73/193 (37.82%), Postives = 109/193 (56.48%), Query Frame = -1 
Query: 1890 YFLGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKKVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEME-QNSECFLLVSD--IKSALYAMRKVIKFFKNSSIRNEILLKHVRQKEGKNLKLILDVKTRWNTLASAVNR 2459
            Y LGL+ I+  C A +   LVE  L ++G+ L  +I+ ST DGASVM KY  +   + Q C NHAIHLAV++  Y K N+ D + D +  + D++E  ++                NL ++ +  N+E    + D  I   L   RK ++FF+ S +RN+IL ++V  KEGK LKLILD +TRWN++   + R
Sbjct:  175 YKLGLIAIECSCDAYQIHSLVEQKLMDYGIKLR-NILTSTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGYH----------NLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSIIPMMER 356

HSP 3 Score: 68.9366 bits (167), Expect = 1.693e-11
Identity = 44/130 (33.85%), Postives = 74/130 (56.92%), Query Frame = -3 
Query: 1579 KRRQELKINFRCKNSLEYIGISCK*ILKLIECGNNALEELGFQK-FDEKYILVLKSISITLEPTMLAVQELSKKDANLITAEGTILYVMKKLKNIDTELSLNLLEILKNEIGKRRNRDMVSLLIFLHQGT 1965
            K  ++LK+   C+     I    + ++ L  C  +ALEE+G    ++EK   +L ++   L+PT LAV+ELSK  + L+TAEG + ++  ++K  +T L       LK  I +RRN+++++L  FL  GT
Sbjct:  332 KEGKKLKLILDCRTRWNSIIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGT 461
[Close]
BLAST of SMED30016927 vs. Planmine SMEST
Match: SMESG000065760.1 (SMESG000065760.1)

HSP 1 Score: 115.931 bits (289), Expect = 1.179e-50
Identity = 70/177 (39.55%), Postives = 107/177 (60.45%), Query Frame = -3 
Query: 2482 MSKRKSEAWNYFQKVNANEAKCNHCGTILSCKGSSTTGLINHLKL-HDLLISRNSKCNDTDSEPSTSKTMKLSNSSIKSFLKSQISLSEILAKFAAKDGFSFNAMIHSDAIKGDVTSCNYSMPKSCTSIQKHVLNFFXXXXXXXXXELIRLLHSKKKFSIVVDEWTDVLMRRFLNVT 3009
            M+ +KSE W  FQK   N AKC  C ++L C G +T+ L NHL+  HD+ + +N        EP  ++    SN  + +F+K + S+ E+LAK +A DGFS +AM HS  I+  +T+ +Y MP+S ++I+K +++F  EKK + K +  ++  S   FSI  DEWT    +R+LNVT
Sbjct:    1 MNSKKSEVWKIFQKETDNTAKCTMCNSVLKCAGGNTSNLANHLRAKHDIDVKKNE-------EPPETRAKATSN--MLNFIKRE-SVGELLAKCSAYDGFSIHAMTHSSVIREFITTRSYVMPRSESTIKKLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCSGKRYLNVT 167

HSP 2 Score: 105.916 bits (263), Expect = 1.179e-50
Identity = 74/194 (38.14%), Postives = 109/194 (56.19%), Query Frame = -1 
Query: 1887 YFLGLVKIKEKCTAEKTKELVENLLKEFGMNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKKVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGNLVMEME-QNSECFLLVSD--IKSALYAMRKVIKFFKNSSIRNEILLKHVRQKEGKNLKLILDVKTRWNTLASAVNRF 2459
            Y LGL+ I+  C A +   LVE  L ++G+ L  +I+AST DGASVM KY  +   + Q C NHAIHLAV++  Y K N+ D + D +  + D++E  ++    D           NL ++ +  N+E    + D  I   L   RK  +F + S +RN+IL ++V  KEGK LKLILD +TRWN++   + R 
Sbjct:  175 YKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKLR-NILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGDH----------NLNIDNDIDNTEYTYSLDDANINEILQNFRKTDRFLRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSIIPMMERL 357
The following BLAST results are available for this feature:
BLAST of SMED30016927 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H9JJ882.409e-4430.16BED-type domain-containing protein OS=Bombyx mori ... [more]
A0A0J7KNG81.272e-4134.21Dimer_Tnp_hAT domain-containing protein OS=Lasius ... [more]
A0A2A4J2351.581e-4130.20BED-type domain-containing protein OS=Heliothis vi... [more]
A0A226DDR73.026e-4133.85Putative AC9 transposase OS=Folsomia candida OX=15... [more]
A0A226D4I77.779e-4133.51Putative AC transposase OS=Folsomia candida OX=158... [more]
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BLAST of SMED30016927 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016927 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022344.11.793e-7035.04SMESG000022344.1[more]
SMESG000026901.16.388e-6740.11SMESG000026901.1[more]
SMESG000008701.16.876e-6290.76SMESG000008701.1[more]
SMESG000077975.13.060e-5140.68SMESG000077975.1[more]
SMESG000065760.11.179e-5039.55SMESG000065760.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016927 ID=SMED30016927|Name=SMED30016927|organism=Schmidtea mediterranea sexual|type=transcript|length=4523bp
TTTTTAATTACGAAATTTATAAAACTTTAATTGCCTAATGAATGTGTATA
ACGTATGAATATTAAACGTGCATGTTTCATGAGTTGCGCCGAGACAGTGA
GTCATCAAAATTCCCTCAAAATCAACTCTATTTCCAAATTTAAAAATGAA
TTTTACGATACATTGAAAATGGTGGACAAAATTGACCGGGCTTATAATTT
AAATGTTAAAATGGCAATTCAGTGGTATCCAGAAATCGCAATAGATTCAG
CGTTGACCGTTACAACATTTCCTTCAACCCAAGTTTCCGTAGAGCGGTTA
TTTTCCGCTCTTAAAATCCTCAATTCTGATTTGAGAGCTTCTACAAATAG
ATTTAATAGAAGCAGTGTTGTTTTTAAGAATAAATTTAAATAAATAATTT
ATAAAATTTAATCATGTTTTATTTTAATGCCTATAAAATGAAAAATTTAG
CTATTTGAAACAGGTAGGAAAACAACCTGATAAAATTAAACTTTTGAGTC
GGATTAAAGTAGTGTTGTTAGTAAATATGGGAATTGAAGTCGGAGTCTTG
TTTTTTTATCGACTCCACAGTCCTTGTTGTAATGCATATTGTTAATTATT
TTAATTTTGATCAAATCTTCGTTAATTATTTGAGAATTAATAAGAATTCC
TTGTATTCTTTCTTTCACTTATGGCTACATAGTTCCATATGTATTTTAAT
TAGAGATGTATTTAATGGACAACATTGTTTGAGTCGGAGATTTTTTACTA
AAACTAAAAATTTATTAAAATACAAATCAGAAACAGTTTATCTTTTTCTA
TTGGAAGCTTTAAAACAGTAACAAGAACGGTATCTTTAAGATGCACATCG
GACAAATTACCAGTAGGGTTTACATTTTACTCGAGTAGTCGAGTACCTTT
TTTAATTAACCAGTTACTCGAGTACTCGAAATAATTTCTAGGAAAAATAA
TTTCTTACCAAAAGATTATCAAAAATACTTATTTATTTAAAAACAAAAAC
AGTTTATTATAACAAGTATAACATAATATAAAATTAAATTAATCCATTAA
ACATTAGAAAAACATTTTAGTCAAAACAAAAATACTTTTTAGTCAAAATA
TTAATTATTTAAATATCTATACATTTTGATTGAAATAATATTTCAAAAAT
ACGAGAGCATTTAACGTATTTACGCCTAGTCGACTTCGTATTTTTGTCAT
TTTGTTTCCTGATACCGAGAATACTCTTTCGCAAGAGGTTGATGTTGGAG
CTACAGTCAATGCATTATATAAGCAATCCATATCGTCACTTCTTTTCCCT
TTATTTCCTTGAAGATTTTAAAATTCTATTTCAATGTTAAATTCATTTTC
TTTAACTTGCTTAGGTCTAGCCAAACTTTGAATACAATTTTGTAAATTTT
CGTATTCCGTTAATTCATCTTCTTCTAAATGTATTATTTCAGTTTCTAAA
TCTTCTTCAGCTTCACGATTTTCAAATTCATTGTGGGGAAACAATCTTAG
AAATAGTTACTTGGCATAATTTTTAAGACTAGATTTAGATGAATAATTTA
AAAACTCATTCTCGTTTTGTTTAACCTAAGTTCCCTGATGTAAAAATATA
AGTAGGGACACCATATCTCTATTTCTACGTTTCCCAATTTCATTTTTTAA
TATTTCTAATAAATTTAGTGATAGTTCAGTATCTATATTCTTAAGTTTCT
TCATAACATATAAGATTGTCCCTTCTGCAGTTATTAAGTTAGCATCTTTT
TTGCTAAGTTCTTGAACTGCTAACATTGTAGGTTCAAGCGTAATTGATAT
GCTTTTTAGAACAAGAATATATTTTTCATCAAACTTTTGAAAACCAAGTT
CTTCTAAGGCATTATTTCCACATTCAATTAACTTTAAAATCTATTTACAG
CTGATGCCAATGTATTCCAACGAGTTTTTACATCTAAAATTAATTTTAAG
TTCTTGCCTTCTTTTTGCCGTACATGCTTTAATAGAATTTCATTACGAAT
CGATGAATTTTTAAAAAATTTAATTACTTTTCGCATTGCATATAACGCTG
ATTTAATATCAGAAACCAATAAAAAACACTCCGAATTTTGTTCCATTTCC
ATTACTAAATTTCCTTCATCTTCTTGATCAACTTGATTTTCTTCATCTTG
ATCATCTTGATCATATAAAAGTTCTTCATCATCATATTCAGTCTTATCTT
CATCTTCAACTACATCATCTAAATTAACTTTTTTATATAAAATTTTAAGG
ACAGCTAGATGAATTGCGTGGTTGTAGCATAATTGTTTTTTTGTTGTAAT
AATTGAACAATACTTCACCATTACACTCGCTCCATCATTTGTTGAGGCAA
CAATATCCGTTTCTAAATTCATTCCAAATTCTTTCAAAAGGTTCTCCACT
AACTCTTTGGTTTTTTCAGCAGTACATTTTTCTTTAATTTTAACTAGTCC
AAGGAAATATTCCTATGAATTTGAAGAGCGCAGTCACATTCAAAAATCTC
CGCATTAAAACATCTGTCCACTCGTCAACTACTATACTAAATTTCTTTTT
TGAATGCAATAATCTTATTAATTCAGTTTTGGTCTCTTGTTTTTTTTCTT
CAAAAAAGTTTAATACATGTTTTTGAATACTAGTACAGCTTTTTGGCATG
CTATAATTACAGCTGGTAACATCGCCTTTAATTGCATCTGAATGAATCAT
AGCATTAAATGAAAATCCATCCTTTGCTGCAAATTTCGCTAAAATTTCTG
ATAAACTTATTTGAGATTTTAAAAAGCTCTTAATTGAAGAATTAGATAAC
TTCATTGTTTTAGATGTTGACGGTTCAGAATCAGTATCATTGCATTTACT
ATTTCTAGAAATAAGCAAATCGTGCAATTTCAAATGGTTTATTAATCCAG
TGGTGCTGCTTCCCTTACAGCTTAGAATTGTTCCGCAGTGATTGCATTTA
GCTTCATTTGCATTGACTTTTTGAAAATAATTCCAAGCTTCTGATTTTCT
TTTACTCATTATGCCTATGGGTAATTAATATGTTAAAAAATGATTAAATA
AAACTATTAGAACTTAACCTTATAAAAAAACTTTTAAAGTTGAAAATGAA
TTATGAATTATGTTAAATCAATCTTTCAACAGATGGCGAATCAGTGTTTA
TATATTATGAAAATTCGATACCCTTTTTAATCGTAGTTTTTTAGTAGTCT
CATTTATATGCATCAAATTTAAGTTATATTTTACAAAAAAATTGTTTTAT
TAAAACTCGAGTACTCGATTCCATTAACTGAGTATTCGAAAACTCGACAA
ATTCGCCAATTTGTCGAGTTATCGAGTAACCGAGTACTCGGAATGGAAAC
CCTAATTACCAGCCGATATAAAAAAATTGAAAGGGAGACATCCAATAAAT
TGGAGTTCGACTGCAGTGGTTTTATATAGTTTTGAATTTTGATGTATTTT
TCTATATTTGAAAAAATATTCATCCAATTATTAATTTACCGTCTCAACTT
GGATATAAAATAGCCAAAATTTTGAAGTTTAGAAAAATCACATCTATTCA
TATAAATTTGCTCTGCAATATAAAAGATTCAGCAATATTTTTATGAATAT
ACAAATATTTTCATTTATATTTATTATGTTGTCCCAAAAGTAAATCACCT
TTTGAAATTTTACTGTTGAAATTCTTTTTGTAATTTAGTAACCTATTGTA
AAAAATATTTATATGAGTTGAAAGGTATAAATATTTTTATTACACTACAC
TATATAGAAGCTATAAAGATATTTTTGTGATGTAGAAAATATGAAAATTG
TTAATAATAATATGTGACAACGATGAATTAAAGATAATGACTAAGGTATC
GAGGAATATATATCTAGGCTTGTCAACAGGTCTCTTTTAAAGAGAACATG
TTCTCTTTTTTAGCACATTTTATTTGTCCTCTATCTTTTTCTATAAATAT
TCAAATTGTTCTCTTTTTTCAATTTCGCTGATTTTTAATTAATTCAAGTT
GAAATTTAAAAAGAAACTCGGTATAAAAATTCAAAAATTTGAAAAAAAGC
AGGCTATGCTATTGCTGTCCTGTCAATATTTATTGAATTTAAAATTAAAG
ATGAAATAATTTGTGTGTATTAACGATTTTGATAAGAAATCATTATTATT
ATAATTTGTTGTGTTAAATAAATAAAATAAGCAAGTGAAAAGTAATTATT
AATATTATATTTTTTTGTGTTAAATAAACAAGTAAGTATGTAATTTATTA
TTGTTAATTAGAGTCTTATATTTTATATTCTTGGATTTTACTATTTTAGC
TCCAAATATGAGTAAACGAAAATGTTTATTTACTGAAGAACTGCAAGCTA
AATTTGTATGTTTTAAATCAACACCAAATTCAAAATCCGAGGCAAAGTAC
AAATTTGAATTATAAGTGCAATTTTATGTTCCTTAATATCTTTATGTATA
AAATTTGATTTTAATAATTTTTTCTTTAAAATGTTCTCTTTTTTTGTGTT
GACCACTTGACAACCCTATCTAT
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protein sequence of SMED30016927-orf-1

>SMED30016927-orf-1 ID=SMED30016927-orf-1|Name=SMED30016927-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=190bp
MSKRKSEAWNYFQKVNANEAKCNHCGTILSCKGSSTTGLINHLKLHDLLI
SRNSKCNDTDSEPSTSKTMKLSNSSIKSFLKSQISLSEILAKFAAKDGFS
FNAMIHSDAIKGDVTSCNYSMPKSCTSIQKHVLNFFEEKKQETKTELIRL
LHSKKKFSIVVDEWTDVLMRRFLNVTALFKFIGIFPWTS*
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protein sequence of SMED30016927-orf-2

>SMED30016927-orf-2 ID=SMED30016927-orf-2|Name=SMED30016927-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp
MNIKRACFMSCAETVSHQNSLKINSISKFKNEFYDTLKMVDKIDRAYNLN
VKMAIQWYPEIAIDSALTVTTFPSTQVSVERLFSALKILNSDLRASTNRF
NRSSVVFKNKFK*
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protein sequence of SMED30016927-orf-3

>SMED30016927-orf-3 ID=SMED30016927-orf-3|Name=SMED30016927-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=163bp
MNLETDIVASTNDGASVMVKYCSIITTKKQLCYNHAIHLAVLKILYKKVN
LDDVVEDEDKTEYDDEELLYDQDDQDEENQVDQEDEGNLVMEMEQNSECF
LLVSDIKSALYAMRKVIKFFKNSSIRNEILLKHVRQKEGKNLKLILDVKT
RWNTLASAVNRF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR036236Znf_C2H2_sf
IPR012337RNaseH-like_sf
IPR003656Znf_BED
IPR008906HATC_C_dom
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0003677DNA binding
GO:0046983protein dimerization activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003656Zinc finger, BED-typeSMARTSM00614bed5coord: 3..49
e-value: 7.9E-12
score: 55.3
IPR003656Zinc finger, BED-typePFAMPF02892zf-BEDcoord: 6..53
e-value: 7.8E-11
score: 41.8
IPR003656Zinc finger, BED-typePROSITEPS50808ZF_BEDcoord: 3..53
score: 11.321
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 6..47