SMED30016911
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016911 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30016911 aligns in the following genomic locations:
Homology
BLAST of SMED30016911 vs. Planmine SMEST
Match: SMESG000025369.1 (SMESG000025369.1) HSP 1 Score: 963.755 bits (2490), Expect = 0.000e+0 Identity = 528/528 (100.00%), Postives = 528/528 (100.00%), Query Frame = 3 Query: 270 MTNMKSFNFLFSKIKIYQVLLHNSFVICVKRTMPQDSIVTQSNRNAVIKFMISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYXXXXXXXXXXXXXXXXXXXNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNXXXXXXXXXXXXMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFLTSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVSKGNNPQTLSVRNLTFLSLSGCITVVVFSIFELVLIIYTHGKPYTEWGCEEWIYRGFDGLKFIFVVINIVSMIHEIRLWLTVEENRFYDLKKLFIYFGTFGNIIKAVILVFFNIFQIVSQSVFENTTLSVNCSYIIMHVEDIIQTVLMSSLILLILDKEPKPFLKISWSYEFCSPILLLMNRVNEIIRCSLYMLIPNGKYFEINLILIGSYSFAQPIVIFYNVTCVIILASTVYSMRH 1853 MTNMKSFNFLFSKIKIYQVLLHNSFVICVKRTMPQDSIVTQSNRNAVIKFMISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYLICLPLLVISIISISLSALNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNFCIIIITICTIFMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFLTSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVSKGNNPQTLSVRNLTFLSLSGCITVVVFSIFELVLIIYTHGKPYTEWGCEEWIYRGFDGLKFIFVVINIVSMIHEIRLWLTVEENRFYDLKKLFIYFGTFGNIIKAVILVFFNIFQIVSQSVFENTTLSVNCSYIIMHVEDIIQTVLMSSLILLILDKEPKPFLKISWSYEFCSPILLLMNRVNEIIRCSLYMLIPNGKYFEINLILIGSYSFAQPIVIFYNVTCVIILASTVYSMRH Sbjct: 1 MTNMKSFNFLFSKIKIYQVLLHNSFVICVKRTMPQDSIVTQSNRNAVIKFMISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYLICLPLLVISIISISLSALNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNFCIIIITICTIFMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFLTSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVSKGNNPQTLSVRNLTFLSLSGCITVVVFSIFELVLIIYTHGKPYTEWGCEEWIYRGFDGLKFIFVVINIVSMIHEIRLWLTVEENRFYDLKKLFIYFGTFGNIIKAVILVFFNIFQIVSQSVFENTTLSVNCSYIIMHVEDIIQTVLMSSLILLILDKEPKPFLKISWSYEFCSPILLLMNRVNEIIRCSLYMLIPNGKYFEINLILIGSYSFAQPIVIFYNVTCVIILASTVYSMRH 528
BLAST of SMED30016911 vs. Planmine SMEST
Match: SMESG000027122.1 (SMESG000027122.1) HSP 1 Score: 160.229 bits (404), Expect = 4.972e-42 Identity = 136/485 (28.04%), Postives = 265/485 (54.64%), Query Frame = 3 Query: 399 RNAVIKFMISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYXXXXXXXXXXXXXXXXXXXNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNXXXXXXXXXXXXMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFLTSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVSKGNNPQTLSVRNLTFLSLSGCITVVVFSIFELVLIIYTHGKPYTEWGCEEWIYRGFDGLKFIFVVINIVS-------MIHEIRLWLTVEENRFYDLKKLFIYFGTFGNIIKAVILVFFNIFQIVSQSVFENTTLSVNCSYIIMHVEDIIQTVLMSSLILLILDKEPKPFLKISWSYEFCSPILLLMNRVNEIIRCSLYMLIPNGKYFEINLILIGSYSFAQPIVIFYNVTCVIILAS 1832 + V K + L S+ L YS FI+ T +++RC + I + I+ + N+LLI+YN ++NY++RNLR +E+P++ YLICLPLL++ +++++L +N+ +W + I ++ G + AVI+ ILDISG +TGEE ++ VN II+T+ ++ K K +I+ N +N S Y++++ +S L+ + + ++E SI++LL+VL PY+++ L ++++ ++K +S + + + + ++L++SG I V V+SI +V I + + + + + F +V+ VS M+ E LW + FY++ +F++FGT G ++ + + F++ +S + +T +++ H+ +++T + S L+L ++++ K L+ W+ F SP+L+ +N+ +I+ L + F+ N IL G S A P I Y++ C ++ AS Sbjct: 2 KEIVEKLIACAYLFTVSLSILLTYSTFIILNTQVQERCSYCFEFIQWPVNILMMVNVLLIVYNTKMNYKYRNLRFSLQIEQPLVFYLICLPLLLVILVALTLLTINSISRWFATRIGFVIEIGLAATEFFAVIILILDISGFSDTGEEPFINSVNSVTIIVTLISMGFLFSKNFILKSRNIFKN----SYNNSIYDKSRDDRSILSYKSNLFLNRLKETSILLLLIVLIPYMMIPLRENLRKLWKSLSISKHKEHIGKKYTSYLTVSGIIIVTVYSIVSIV--IGVNRNNFIK-----------NPIYFFLMVVQEVSVFPIFYLMLQETNLWTRLMNVNFYNITNIFLHFGTIGQYLQTLNGILFHVI----ESQMKASTNNIDYFMFGFHLIKLLKTTITSLLVLAMVERNFKKPLRTFWNCFFSSPVLIFINQWANLIQ-ELMQQVDYKNGFQANAILQGVTSLASPFCILYHLYCSMMFAS 464
BLAST of SMED30016911 vs. Planmine SMEST
Match: SMESG000025336.1 (SMESG000025336.1) HSP 1 Score: 157.532 bits (397), Expect = 6.625e-41 Identity = 142/496 (28.63%), Postives = 271/496 (54.64%), Query Frame = 3 Query: 366 MPQDSIVTQSNRNAVIKFMISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYXXXXXXXXXXXXXXXXXXXNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNXXXXXXXXXXXXMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFLTSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVSKGNNPQTLSVRNLTFL-SLSGCITVVVFSIFELVLIIYTHGKPYTEWGCEEWIY---RGFDGLKFIFVVINIVSMIHEIRLWLTVEENRFYDLKKLFIYFGTFGNIIKAVILVFFNIF--QIVSQSVFENTTLSVNCSYIIMHVEDIIQTVLMSSLILLILDKEPKPFLKISWSYEFCSPILLLMNRVNEIIRCSLYMLIP-NGK--YFEINLILIGSYSFAQPIVIFYNVTCVIIL 1826 M + +KF+IS+ L +F +VY+TNY ++ R TP ++R G + I ++ VK+ +I+LI YN +IN+RFRNLRI++ +E+P+I YLICLP+L++S+I + + L T + + I I+ ++DI+G +TGEEW M+Y N IIIT+ ++ M+ + ++ +SN + + + + Y + F +F ++ N+I +++ ++I L + P+L++ L +++ ++F+ +K + P+ + ++ + S++G +TV V+ L+ I+ E G +IY R +F + + MI EI++ + +++ ++K + IY G FG ++ + +I+ +I +FE L N ++ E +I+T+L+++L+L +LD E FL+ SW++ F +PI +L+ +++ S Y+ P GK F+I +I G+ FA +V Y C +I+ Sbjct: 1 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYRITPNENRNDGYIEKINYSISFVKVVSIILICYNTKINHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSMTIIITMTSVIMQICLNIIMILTEEFSNSLNQVLHFNLYSNHSF--TFFDKTSYNKIIFLKKRTVITLSLAFIPFLVIPLKRNLNHLFQFKTKDDLPEDQPILSMNKVSSITGSLTVGVY--ITLIFIVDLCNFVIHERG--NYIYLTSRIIKAFLILFSIGLLAIMITEIKVSARLNSSQYLNVKNILIYLGAFGQFSISIAYIVVDIYNLEINGIGLFE---LIQNILLLVSSFEYLIETMLLTTLMLFVLDGETIFFLENSWTFFFHTPINILILQLSIF---SEYLTKPVYGKENSFQIRIIEQGTLDFAIYLVYLYRSICFMII 484
BLAST of SMED30016911 vs. Planmine SMEST
Match: SMESG000025344.1 (SMESG000025344.1) HSP 1 Score: 155.992 bits (393), Expect = 2.363e-40 Identity = 142/495 (28.69%), Postives = 259/495 (52.32%), Query Frame = 3 Query: 366 MPQDSIVTQSNRNAVIKFMISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYXXXXXXXXXXXXXXXXXXXNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNXXXXXXXXXXXXMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFLTSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVSKGNNPQTLSVRNLTFL-SLSGCITVVVFSIFELVLIIYTHGKPYTEWGCEEWI--YRGFDGLKFIFVVINIVSMIHEIRLWLTVEENRFYDLKKLFIYFGTFGNIIKAVILVFFNIF--QIVSQSVFENTT----LSVNCSYIIMHVEDIIQTVLMSSLILLILDKEPKPFLKISWSYEFCSPILLLMNRVN---EIIRCSLYMLIPNGKYFEINLILIGSYSFAQPIVIFYNVTC 1814 M + +KF+IS+ L +F +VY+TNY ++ + +P DR I ++ VK+ +I+LI YN +IN+RFRNLRI++ +E+P+I YLICLP+L IS++ + + + T + S ++ + F I I+ ++DI+G +TGEEW M+Y N IIIT+ ++ ++ + ++ ++N + + +S + + F + + + +++ ++I L +V P+L++ L +++ ++F+ K + P+ +V ++ + S++G TV VF L I+ H Y + I R L F+ + MI EI++ + +++ ++K + IY G FG + + NI+ Q +FE+T L+ N Y +I+T+LM++L+L +LD + F K SWS F SPI +L+ ++ E + S Y F+I ++ IG+ FA P+VI Y C Sbjct: 1 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPMLFISLMGLFVIIFHYTFEKSGTSTNI-LYFALVIFEYFLFIILVIDINGFSDTGEEWFMNYFNSMTIIITMTSVMIQICLNIIMILTDDFTNTMNRTTFVSLFSNHNF--TIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNKVSSITGGFTVGVF----LTFIVIVH-LLYVMQNVPQVIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNIYNLQTNGNGLFESTKDILILTSNFVY-------LIETILMTTLMLFVLDGKAVSFPKNSWSIFFYSPINILILQIGVFTEYLTKSFY---DEQNSFQIRMMEIGTMYFAIPLVILYRAIC 477
BLAST of SMED30016911 vs. Planmine SMEST
Match: SMESG000025342.1 (SMESG000025342.1) HSP 1 Score: 114.775 bits (286), Expect = 1.587e-27 Identity = 83/287 (28.92%), Postives = 167/287 (58.19%), Query Frame = 3 Query: 366 MPQDSIVTQSNRNAVIKFMISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYXXXXXXXXXXXXXXXXXXXNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNXXXXXXXXXXXXMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFLTSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVSKGNNPQTLSVRNLTFL-SLSGCITVVVF 1223 M + +KF+IS+ L +F +VY+TNY ++ R +P+++R G + I ++ VK+ +I+LI YN +IN+RFRNLRI++ +++P+I YLICLP+L++S+I + + L T + + I I+ ++DI+G +TGEEW M+Y N IIIT+ ++ M+ + ++ + N + + + + Y + F +F ++ N+I +++ ++I L + P+L++ L +++ ++F+ +K + P+ + ++ + S++G +TV V+ Sbjct: 1 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYRISPMENRNDGYIEKINYSISFVKVVSIILICYNTKINHRFRNLRIKHKLKQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSMTIIITMTSVIMQICLNIIMILTDEFFNSLNQVLHFNLYSNHSF--TFFDKTSYNKIIFLKKRTVITLSLAFIPFLVIPLKRNLNHLFQFKTKDDLPEDQPILSMNKVSSITGSLTVGVY 285 The following BLAST results are available for this feature:
BLAST of SMED30016911 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30016911 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30016911 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30016911 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30016911 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016911 ID=SMED30016911|Name=SMED30016911|organism=Schmidtea mediterranea sexual|type=transcript|length=2341bpback to top protein sequence of SMED30016911-orf-1 >SMED30016911-orf-1 ID=SMED30016911-orf-1|Name=SMED30016911-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=545bp MINKFHRQNKRNYLITMTNMKSFNFLFSKIKIYQVLLHNSFVICVKRTMPback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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