CRAL-TRIO domain-containing protein

Overview
NameCRAL-TRIO domain-containing protein
Smed IDSMED30016902
Length (bp)2099
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of CRAL-TRIO domain-containing protein (SMED30016902) t-SNE clustered cells

Violin plots show distribution of expression levels for CRAL-TRIO domain-containing protein (SMED30016902) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of CRAL-TRIO domain-containing protein (SMED30016902) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for CRAL-TRIO domain-containing protein (SMED30016902) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30016902

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30016902h1SMcG0022483 SmedASXL_068046SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30016902h1SMcG0022483 dd_Smed_v4_2760_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016902h1SMcG0022483 dd_Smed_v4_2760_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016902h1SMcG0022483 dd_Smed_v4_2760_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30016902h1SMcG0022483 dd_Smed_v6_2760_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30016902h1SMcG0022483 dd_Smed_v6_2760_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016902h1SMcG0022483 dd_Smed_v4_2760_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: TTPAL (alpha tocopherol transfer protein like [Source:HGNC Symbol;Acc:HGNC:16114])

HSP 1 Score: 109.768 bits (273), Expect = 2.097e-25
Identity = 73/256 (28.52%), Postives = 125/256 (48.83%), Query Frame = 2
Query: 1061 GISEKTKQFAIDDIGENPSQVNAHIESFRRWL-KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
             ++E     A +++ E P      +++ R  + K  P+L    D  FL++FLR  KFD+ RA + + NY + R   R  P  FN  ++    +   L +G    L  T+  G  +  +R   W P   P  + I  +++ ++ +   EETQ++G+ ++ D K  S      +G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EK+ +R     +D+N      P    ILP EYGG  G +
Sbjct:   34 SLTEDLVTKAREELQEKPEWRLRDVQALRDMVRKEYPNLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHSCR---RSWPEVFN--NLKPSALKDVLASGFLTVLPHTDPRGCHVVCIRPDRWIPSNYPITENIRAIYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFG-PFIAKKVIGILQDGFPIRIKAVHVVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RSILPKEYGGTAGEL 281          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: TTPAL (alpha tocopherol transfer protein like [Source:HGNC Symbol;Acc:HGNC:16114])

HSP 1 Score: 109.768 bits (273), Expect = 2.097e-25
Identity = 73/256 (28.52%), Postives = 125/256 (48.83%), Query Frame = 2
Query: 1061 GISEKTKQFAIDDIGENPSQVNAHIESFRRWL-KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
             ++E     A +++ E P      +++ R  + K  P+L    D  FL++FLR  KFD+ RA + + NY + R   R  P  FN  ++    +   L +G    L  T+  G  +  +R   W P   P  + I  +++ ++ +   EETQ++G+ ++ D K  S      +G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EK+ +R     +D+N      P    ILP EYGG  G +
Sbjct:   34 SLTEDLVTKAREELQEKPEWRLRDVQALRDMVRKEYPNLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHSCR---RSWPEVFN--NLKPSALKDVLASGFLTVLPHTDPRGCHVVCIRPDRWIPSNYPITENIRAIYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFG-PFIAKKVIGILQDGFPIRIKAVHVVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RSILPKEYGGTAGEL 281          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: TTPA (alpha tocopherol transfer protein [Source:HGNC Symbol;Acc:HGNC:12404])

HSP 1 Score: 96.2857 bits (238), Expect = 2.262e-21
Identity = 67/235 (28.51%), Postives = 111/235 (47.23%), Query Frame = 2
Query: 1163 MPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWG-------DPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKW 1843
            +P    P    FL++FLR   FD   A + + NY   RA     P    +  ++   I   L+ G H  L  R   G  + + R   WDP+      +  +  +  ++I  + ETQ +G+K I DL        E W         P   K+I  V+ +S+PL+++G    NEPVIF  VF ++  F+ EK+K R     N+  +++ ++     ILP+EYGG+  ++ +  ++W
Sbjct:   40 VPLAPLPLTDSFLLRFLRARDFDLDLAWRLLKNYYKWRAE---CPEI--SADLHPRSIIGLLKAGYHGVLRSRDPTGSKVLIYRIAHWDPKVFTAYDVFRVSLITSELIVQEVETQRNGIKAIFDL--------EGWQFSHAFQITPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKERIHMHGNNYKQSLLQH--FPDILPLEYGGEEFSMEDICQEW 259          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: CLVS2 (clavesin 2 [Source:HGNC Symbol;Acc:HGNC:23046])

HSP 1 Score: 90.1225 bits (222), Expect = 6.437e-19
Identity = 65/253 (25.69%), Postives = 121/253 (47.83%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTEN-GEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK-NPGLDKILPMEYGG 1807
            +G+S +T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF H  A + +  Y   R   + +   F +F   DP I   L+ G    L   ++ G  I ++    WD  +     ++  + ++++ +  D E Q++G  +IID  + + K       P  ++     +Q+S+P R  G  F N+P     ++ V+  F+ EK + R     N++N   +  +P   +ILP E+GG
Sbjct:    6 AGLSPETLEKARLELNENPDTLHQDIQEVRDMVITRPDIGFLRTDDAFILRFLRARKFHHFEAFRLLAQYFEYR---QQNLDMFKSFKATDPGIKQALKDGFPGGLANLDHYGRKILVLFAANWDQSRYTLVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLT-PSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKEKTRKRIFLHGNNLNSLHQLIHP---EILPSEFGG 251          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: CLVS1 (clavesin 1 [Source:HGNC Symbol;Acc:HGNC:23139])

HSP 1 Score: 88.9669 bits (219), Expect = 2.080e-18
Identity = 67/253 (26.48%), Postives = 119/253 (47.04%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK-NPGLDKILPMEYGG 1807
            +G+S +T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF    A + +  Y   R   + +   F  F  +DP I   L  G    L  R   G  I L+    WD  +  F  ++  + ++++++  D E QI+G  +IID  + S K       P  +K     +Q+S+P R  G  F N+P     ++ ++  F+ +K + R     N++N   +  +P   + LP E+GG
Sbjct:   28 AGLSPETIEKARLELNENPDVLHQDIQQVRDMIITRPDIGFLRTDDAFILRFLRARKFHQADAFRLLAQYFQYR---QLNLDMFKNFKADDPGIKRALIDGFPGVLENRDHYGRKILLLFAANWDQSRNSFTDILRAILLSLEVLIEDPELQINGFILIIDWSNFSFKQASKL-TPSILKLAIEGLQDSFPARFGGVHFVNQPWYIHALYTLIKPFLKDKTRKRIFLHGNNLNSLHQLIHP---EFLPSEFGG 273          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG33966 (gene:FBgn0053966 transcript:FBtr0100007)

HSP 1 Score: 119.783 bits (299), Expect = 2.111e-29
Identity = 82/315 (26.03%), Postives = 157/315 (49.84%), Query Frame = 2
Query: 1064 ISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSIN-DPIINTY-LETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQI-HGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKF 1999
            +S + ++ AI+++ E P++++  I + R W+K  PHL+  TD +FL+ FLR  KF  +R + +ID + T+R      P F+   +++ D  +  + L T + LP    +NG  + L+R   +DP +    ++     +   I+ ++++  I +G+  I+DL + +         P + K++    +E+ PLR +G  F N P  FD +F ++   M++K + R     +       + P   + LP+EYGG+ G+I E  ++W+ R   +         +  DES R       + +  D+E    G QG++ + 
Sbjct:    7 LSPELQKTAIENLNEVPNKLDDDIAALRDWIKQQPHLKARTDDQFLVNFLRGCKFSLERTKSKIDRFYTLRTK---YPEFYLGHNVDVDKALEIFRLGTIVILPRPLNDNGPRLALLRMACYDPSKYTLQEVNRAAGLMQQIMLDEDDVAIVNGLISILDLSNVTTGHFLQMS-PSFAKKMTVFQEEALPLRPQGIHFINTPNGFDTIFNMIKPMMSKKQQGRLYVHGSKWEALYNQIP--KQYLPVEYGGENGSIPELLQQWEQRILAYRNYWEEEKNYGTDESLR-------VGQPVDFES-LFGLQGSFRQL 307          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG33514 (gene:FBgn0053514 transcript:FBtr0091456)

HSP 1 Score: 117.087 bits (292), Expect = 1.421e-28
Identity = 87/320 (27.19%), Postives = 150/320 (46.88%), Query Frame = 2
Query: 1064 ISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETG--MHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFM----AMD--IICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKF 1999
            ++ + ++ A + + E+P ++ A +++F+ W++  PHL    D +FL+ FLR  K+  +RA+ ++D Y T++      P +F   +  D       +TG  ++LP    ENG      R  +W    +P  K  +L  M    AM    I  D+   ++G+  I+D+K A+   +     P   K+     +E+ PLR+K   F N    F+ +F +    M++KM+SR     N +    E+ P   K LP EYGG+ GT  +     + +  E+         F  DES RP +         D+EG   G +G++ K 
Sbjct:    8 LTPELQKVAKEQLKEDPERLEADLQAFKTWIEQQPHLNPRMDDQFLVAFLRGCKYSLERAKSKLDKYYTLKTK---YPDYFRVTNTTDSKFREIHQTGAIIYLPTPLNENGP-----RIGIWRMGLVPVEKYTMLECMQVAQAMQEIAILEDDYANVNGVVFIMDMKGATAAHLFQM-TPSMAKKFTVFSEEALPLRLKAQHFINTITGFEQLFNMFKPMMSKKMQSRLFVHGNKMGLLTEQIPL--KYLPEEYGGENGTTQDIVAAMEKKLDEYADFFQENVNFGTDESLRPGKPI-------DFEG-LFGVEGSFRKL 308          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG12926 (gene:FBgn0033437 transcript:FBtr0088502)

HSP 1 Score: 107.071 bits (266), Expect = 3.921e-25
Identity = 81/308 (26.30%), Postives = 148/308 (48.05%), Query Frame = 2
Query: 1037 MTERYEYS--GISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETG--MHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDI-ICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIR---FKEFY------------KNGNPVSGFSV 1900
            M+E Y  S   +S +  + A D++GE P +++  IE+ R W+   PHL+   DA+FL+ FLR  K+  ++ + ++DN+  +R +    P  +    + +  ++  L+TG  + LP     +G  I + R   +D ++    +++ +  M  +I I  D+   I G   IID+K      +  + D   +K++  +  ++YP R KG  F N P   +    +    M+EK++ R   I + ++   +  P   + LP EYGG  GTI +    W+ +   +K F+            + G PVS  S+
Sbjct:    1 MSEAYSNSIRSLSPELAKKAHDELGEIPDRIDEDIETLRTWISKQPHLKARQDAQFLVAFLRGCKYSLEKTKLKLDNFYAMRGA---VPELYKNRIVGEKQLSI-LDTGCLLRLPQPLQADGPRIHISRYGQYDSKKYSIAEVVQVNTMLGEIQIREDDNAMISGFVEIIDMKGVGAGHLFQF-DAVLVKKLAVLGDKAYPYRPKGFHFVNAPSSAEKFMSIAKSLMSEKIRKR-FHIHSKLDSLYKYVP--KECLPAEYGGSNGTIQDVVSTWRTKLLAYKPFFEEEASYGTNEKLRRGQPVSAESL 300          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG31636 (gene:FBgn0051636 transcript:FBtr0079324)

HSP 1 Score: 105.916 bits (263), Expect = 1.046e-24
Identity = 72/274 (26.28%), Postives = 134/274 (48.91%), Query Frame = 2
Query: 1064 ISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSI-NDPIINTYLETG--MHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIIC-NDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKND---INKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEF 1864
            ++   ++  I ++ E P+++   IE+ R W+   PHL+  TD +FL+ FLR +KF  +RA+++ D + T++   R  P  FN   +  DP +   +  G  + +P+   + G  + ++R   +D  +  F  +I +  M  +I+   D+   + G   I+D+   +   + +   P+ + + +    E+ P R KG  F N P  F+  F  L  F   K+KSR I + +D   I + + +     K LP EYGG GG + + S   + +   +
Sbjct:    7 LNAALQEICIRELNELPARMAQDIEALRDWVLKQPHLRACTDDQFLLAFLRGTKFSLERAKEKFDRFYTLQ---RSIPEVFNERRLATDPQVLDIVRMGVLLQIPMDADDPGPRVTIIRAGSYDTSKHKFQDIIRVGSMFGEIMMFEDDNATVSGYVEIMDMAGVTGSHLFAL-QPQLLSKFSTYADEAMPTRQKGIHFINVPAAFETGFNSLRSFFPAKIKSR-ISVSSDPAAIYELVRR-----KYLPQEYGGTGGNLQDISHTMEAKLSSY 270          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG31636 (gene:FBgn0051636 transcript:FBtr0343865)

HSP 1 Score: 105.916 bits (263), Expect = 1.046e-24
Identity = 72/274 (26.28%), Postives = 134/274 (48.91%), Query Frame = 2
Query: 1064 ISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSI-NDPIINTYLETG--MHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIIC-NDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKND---INKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEF 1864
            ++   ++  I ++ E P+++   IE+ R W+   PHL+  TD +FL+ FLR +KF  +RA+++ D + T++   R  P  FN   +  DP +   +  G  + +P+   + G  + ++R   +D  +  F  +I +  M  +I+   D+   + G   I+D+   +   + +   P+ + + +    E+ P R KG  F N P  F+  F  L  F   K+KSR I + +D   I + + +     K LP EYGG GG + + S   + +   +
Sbjct:    7 LNAALQEICIRELNELPARMAQDIEALRDWVLKQPHLRACTDDQFLLAFLRGTKFSLERAKEKFDRFYTLQ---RSIPEVFNERRLATDPQVLDIVRMGVLLQIPMDADDPGPRVTIIRAGSYDTSKHKFQDIIRVGSMFGEIMMFEDDNATVSGYVEIMDMAGVTGSHLFAL-QPQLLSKFSTYADEAMPTRQKGIHFINVPAAFETGFNSLRSFFPAKIKSR-ISVSSDPAAIYELVRR-----KYLPQEYGGTGGNLQDISHTMEAKLSSY 270          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: ttpal (tocopherol (alpha) transfer protein-like [Source:ZFIN;Acc:ZDB-GENE-030131-296])

HSP 1 Score: 107.457 bits (267), Expect = 2.114e-25
Identity = 68/224 (30.36%), Postives = 109/224 (48.66%), Query Frame = 2
Query: 1154 LKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
            LK  P+L+   D  FL++FLR  KFD+ RA + + NY    AS R  P  F    +    +   L+ G    L R +  G  I  +R   W P   PF   I  +++ ++ +   EETQ++G+ +++D          + G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EKM  R +   +D+       P    +LP EYGG  G +
Sbjct:    3 LKDHPNLRTRLDDAFLLRFLRARKFDYDRALQLLLNY---HASRRAWPEVFQ--DLKPSTVKHVLDLGFLTVLPRPDPQGRYILCLRPGKWKPNDYPFVDNIRAIYLTLEKLIQPEETQVNGVVILVDYAGVGLSQASNPG-PLLAKKVVGILQDGFPIRIKAVNIINEPRIFKGIFAIIKPFLKEKMAERYVLHGSDLASLHRVIP--QSVLPQEYGGVSGRL 218          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: ttpal (tocopherol (alpha) transfer protein-like [Source:ZFIN;Acc:ZDB-GENE-030131-296])

HSP 1 Score: 108.227 bits (269), Expect = 3.549e-25
Identity = 72/247 (29.15%), Postives = 119/247 (48.18%), Query Frame = 2
Query: 1088 AIDDIGENPSQVNAHIESFRRW-LKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
            A +++ E P      +++ R   LK  P+L+   D  FL++FLR  KFD+ RA + + NY    AS R  P  F    +    +   L+ G    L R +  G  I  +R   W P   PF   I  +++ ++ +   EETQ++G+ +++D          + G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EKM  R +   +D+       P    +LP EYGG  G +
Sbjct:   42 AREELQEKPEWRLRDVQALRDMILKDHPNLRTRLDDAFLLRFLRARKFDYDRALQLLLNY---HASRRAWPEVFQ--DLKPSTVKHVLDLGFLTVLPRPDPQGRYILCLRPGKWKPNDYPFVDNIRAIYLTLEKLIQPEETQVNGVVILVDYAGVGLSQASNPG-PLLAKKVVGILQDGFPIRIKAVNIINEPRIFKGIFAIIKPFLKEKMAERYVLHGSDLASLHRVIP--QSVLPQEYGGVSGRL 280          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: clvs2 (clavesin 2 [Source:NCBI gene;Acc:566769])

HSP 1 Score: 87.8113 bits (216), Expect = 2.155e-18
Identity = 64/252 (25.40%), Postives = 117/252 (46.43%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTEN-GEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGG 1807
            +G+S +T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF+H  A + +  Y   R   + +   F      DP I   L+ G    L   +  G  I ++    WD  +  F  ++  + ++++ +  D E Q++G  +IID  + + K       P  ++     +Q+S+P R  G  F N+P     ++ V+  F+ +K + R     N++N        L +ILP E GG
Sbjct:    6 AGLSPETLEKAKVELKENPDTLHQDIQEVRDMIITRPDIGFLRTDDAFILRFLRARKFNHFEAFRLLAQYFEYR---QQNLDMFKNLKATDPGIKQALKDGFPGVLSNLDRYGRKILVLFAANWDQSRYTFVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLT-PSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKDKTRKRIFMHGNNLNSL--HQLILPEILPSELGG 251          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: ttpa (tocopherol (alpha) transfer protein [Source:ZFIN;Acc:ZDB-GENE-030131-4631])

HSP 1 Score: 82.0333 bits (201), Expect = 9.909e-17
Identity = 59/218 (27.06%), Postives = 108/218 (49.54%), Query Frame = 2
Query: 1196 FLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKD-ASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKW 1843
            FL++FL+   FD   A K + NY   R   +  P    T  +    +   L+   H  L  R + G  + + R   W+P++    ++  +  +  ++I  + ETQ +G+K I DL+D     A++   +P   K+I+ V+ +S+PL+++G    NEP+ F  VF ++  F+ +K+K R         +++  N     +LP  YGG G ++ E  ++W
Sbjct:   48 FLIRFLQARDFDVALALKLLINYHKWR---QECPEI--TADLRPSSVIGLLQNNYHGVLRSRDDAGSRVLIYRIGKWNPKEFTAYEVFRVSLITSELIVQEWETQRNGLKAIFDLQDWCFAHALQI--NPSLAKKISSVLTDSFPLKVRGIHLINEPIFFRPVFAMIRPFLPDKIKQRIHMHGCSYARSL-CNYFPKAVLPPVYGGTGPSVDEVCQEW 257          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: rlbp1a (retinaldehyde binding protein 1a [Source:ZFIN;Acc:ZDB-GENE-040426-1662])

HSP 1 Score: 75.485 bits (184), Expect = 2.475e-14
Identity = 63/262 (24.05%), Postives = 120/262 (45.80%), Query Frame = 2
Query: 1064 ISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMP----HLQCPTDAKF-------LMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLG-RTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQG 1813
            + + TKQ A D++ E   +  + I+  R  +K        +      KF       L++F+R  KFD  RA + +  Y   R   R  P  F   ++    + + +E G    L  R +NG V+ L     WD E++ F + +    + ++ +  +EETQI+G  +I + K  + +        +  K ++ ++Q+S+P R K     ++P  F   + V+  FM  K+  R     ++++  + ++ G + ILP ++ G G
Sbjct:   39 LPDHTKQKAKDELNETDEKRASAIKELRAMIKDKAGQGDEVAKTVQDKFSKEPDSLLLRFIRARKFDVARAHELMKGYVRFR---RDYPELFE--NLTPEAVRSTIEAGYPRILSTRDKNGRVVLLFNIDNWDLEEVTFDETLRAYCVILEKLLENEETQINGFVLIENFKGFTMQHASGIKHTELKKMVD-MLQDSFPARFKAVHVIHQPWYFTTTYNVVKPFMKSKLLERVFVHGDELDGYL-RDFGAE-ILPPDFDGTG 292          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: ttpal (tocopherol (alpha) transfer protein-like [Source:NCBI gene;Acc:548930])

HSP 1 Score: 109.768 bits (273), Expect = 1.485e-25
Identity = 74/248 (29.84%), Postives = 122/248 (49.19%), Query Frame = 2
Query: 1088 AIDDIGENPSQVNAHIESFRRWL-KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTF--SINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
            A +++ E P      +++ R  + K  PHL+   D  FL++FLR  KFD+ RA + + NY + R   +  P  F     S   P++++   T   LP   TE G  I  +R   W P   P  + I  ++++++ +   EETQ++G+ ++ D           +G P   K++  ++Q+ +P+RIK     NEP IF  +F +L  F+ EK+  R     +D+N      P    ILP EYGG  G +
Sbjct:   40 AREELQEKPEWRLRDVQALRDMVWKDYPHLKTRVDDSFLLRFLRARKFDYDRALQLLVNYYSCR---KAWPEVFTDLRPSAVKPVLDSGFLTV--LPHTDTE-GRRIVCIRPGCWIPRDYPITENIRAIYLSLEKLVESEETQVNGIVILADYNGVGLTHASHFG-PFIAKKVIGILQDGFPIRIKAVNVINEPRIFKGIFAILKPFLKEKIVKRFFLHGSDLNSLHANIP--KSILPEEYGGTAGKL 278          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: ggh (gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) [Source:NCBI gene;Acc:594986])

HSP 1 Score: 95.1301 bits (235), Expect = 6.228e-21
Identity = 65/217 (29.95%), Postives = 108/217 (49.77%), Query Frame = 2
Query: 1196 FLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKW 1843
            FL++FLR   F  + A K + NY   RA     P          PI++ +   G H  L  R ++G  + + R + WDP+     ++  +  +  ++I  + ETQ +G+K I DL+   R A      P   K+I  VM +S+PL+++G    NEP+ F  VF ++  F+ +K+K R     ++    + ++     ILP EYGG G  ++E  E+W
Sbjct:   51 FLLRFLRARDFCIELAFKLLKNYHKWRAE---CPEITADLR-PSPILDLF-RAGYHAVLRSRDDSGSKVLIYRIEYWDPKLFTAYEVFRVSLITSELIVQEAETQRNGIKAIFDLQ-GWRLAHAFQITPTMAKRIAAVMADSFPLKVRGVHLINEPLFFHPVFAIIKPFLPDKIKQRVHMHGSNYIPTLNQHFS-TSILPPEYGGTGPAMSELCEEW 260          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: thg1l (tRNA-histidine guanylyltransferase 1-like [Source:Xenbase;Acc:XB-GENE-966760])

HSP 1 Score: 89.3521 bits (220), Expect = 8.781e-19
Identity = 65/253 (25.69%), Postives = 121/253 (47.83%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTEN-GEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK-NPGLDKILPMEYGG 1807
            +G+S +T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF+H  A + +  Y   R   + +   F  F   DP I   L+ G    L   ++ G  I ++    WD  +     ++  + ++++ +  D E Q++G  +IID  + + K       P  ++     +Q+S+P R  G  F N+P     ++ V+  F+ EK + R     N++N   +  +P   +ILP E+GG
Sbjct:    6 AGLSPETLEKARLELNENPDTLHQDIQEVRDMVITRPDIGFLRTDDAFILRFLRARKFNHFEAFRLLAQYFEYR---QQNLDMFKNFKATDPGIKQGLKDGFPGVLSNLDHFGRKILVLFAANWDQSRYTLVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLT-PSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKEKTRKRIFLHGNNLNSLHQLIHP---EILPSEFGG 251          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: clvs1 (clavesin 1 [Source:NCBI gene;Acc:100037913])

HSP 1 Score: 88.9669 bits (219), Expect = 1.309e-18
Identity = 67/262 (25.57%), Postives = 125/262 (47.71%), Query Frame = 2
Query: 1031 IKMTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK-NPGLDKILPMEYGG 1807
            +KMT  + ++G+S +T + +  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF+   A + +  Y   R   + +   F     +DP I   L  G    L  R   G  I L+    WD  +  F  ++  + ++++++  D+E QI+G  +IID  + S K       P  ++     +Q+S+P R  G  F N+P     ++ ++  F+ +K + R     N++N   +  +P     LP E+GG
Sbjct:   18 LKMT--HLHAGLSPETIEKSRLELNENPDTLHQDIQQVRDMIITRPDIGFLRTDDAFILRFLRARKFNQMEAFRLLAQYFQYR---QLNLDMFKNLKADDPGIKRALMDGFPGVLENRDHYGRKILLLFAANWDQSRNSFVDILRAILLSLEVLIEDQELQINGFILIIDWSNFSFKQASKLT-PSILRLAIEGLQDSFPARFGGVHFVNQPWYIHALYTIIKPFLKDKTRKRIFLHGNNLNSLHQLIHP---DCLPSEFGG 270          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: clvs1 (clavesin 1 [Source:NCBI gene;Acc:100037913])

HSP 1 Score: 87.8113 bits (216), Expect = 3.671e-18
Identity = 64/254 (25.20%), Postives = 120/254 (47.24%), Query Frame = 2
Query: 1055 YSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK-NPGLDKILPMEYGG 1807
            ++G+S +T + +  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF+   A + +  Y   R   + +   F     +DP I   L  G    L  R   G  I L+    WD  +  F  ++  + ++++++  D+E QI+G  +IID  + S K       P  ++     +Q+S+P R  G  F N+P     ++ ++  F+ +K + R     N++N   +  +P     LP E+GG
Sbjct:    5 HAGLSPETIEKSRLELNENPDTLHQDIQQVRDMIITRPDIGFLRTDDAFILRFLRARKFNQMEAFRLLAQYFQYR---QLNLDMFKNLKADDPGIKRALMDGFPGVLENRDHYGRKILLLFAANWDQSRNSFVDILRAILLSLEVLIEDQELQINGFILIIDWSNFSFKQASKLT-PSILRLAIEGLQDSFPARFGGVHFVNQPWYIHALYTIIKPFLKDKTRKRIFLHGNNLNSLHQLIHP---DCLPSEFGG 251          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Ttpal (tocopherol (alpha) transfer protein-like [Source:MGI Symbol;Acc:MGI:1923330])

HSP 1 Score: 108.227 bits (269), Expect = 3.886e-25
Identity = 73/260 (28.08%), Postives = 125/260 (48.08%), Query Frame = 2
Query: 1061 GISEKTKQFAIDDIGENPSQVNAHIESFRRWL-KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECS 1834
             ++E     A +++ E P      +++ R  + K  P+L    D  FL++FLR  KFD+ RA + + NY   R   R  P  F+  ++    +   L +G    L  T+  G  +  +R   W P   P  + I  +++ ++ +   EETQ++G+ ++ D K  S      +G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EK+ +R     +D+N      P    ILP EYGG  G +   S
Sbjct:   35 SLTEDLVTKAREELQEKPEWRLRDVQALRDMVRKEYPYLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHGCR---RSWPEVFS--NLRPSALKDVLNSGFLTVLPHTDPRGCHVLCIRPDRWIPSNYPITENIRAVYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFG-PFIAKKVIGILQDGFPIRIKAVHIVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RNILPKEYGGTAGELDTAS 286          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Ttpal (tocopherol (alpha) transfer protein-like [Source:MGI Symbol;Acc:MGI:1923330])

HSP 1 Score: 108.227 bits (269), Expect = 3.886e-25
Identity = 73/260 (28.08%), Postives = 125/260 (48.08%), Query Frame = 2
Query: 1061 GISEKTKQFAIDDIGENPSQVNAHIESFRRWL-KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECS 1834
             ++E     A +++ E P      +++ R  + K  P+L    D  FL++FLR  KFD+ RA + + NY   R   R  P  F+  ++    +   L +G    L  T+  G  +  +R   W P   P  + I  +++ ++ +   EETQ++G+ ++ D K  S      +G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EK+ +R     +D+N      P    ILP EYGG  G +   S
Sbjct:   35 SLTEDLVTKAREELQEKPEWRLRDVQALRDMVRKEYPYLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHGCR---RSWPEVFS--NLRPSALKDVLNSGFLTVLPHTDPRGCHVLCIRPDRWIPSNYPITENIRAVYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFG-PFIAKKVIGILQDGFPIRIKAVHIVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RNILPKEYGGTAGELDTAS 286          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Ttpa (tocopherol (alpha) transfer protein [Source:MGI Symbol;Acc:MGI:1354168])

HSP 1 Score: 93.9745 bits (232), Expect = 8.585e-21
Identity = 67/236 (28.39%), Postives = 108/236 (45.76%), Query Frame = 2
Query: 1160 SMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWG-------DPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKW 1843
             +P    P    FL++FLR   FD   A + + NY   RA     P    +  +    I   L+ G H  L  R   G  + + R   WDP+      +  +  +  ++I  + ETQ +G+K I DL        E W         P   K+I  V+ +S+PL+++G    NEPVIF  VF ++  F+ EK+K R     N+   ++ ++     ILP EYGG+  ++ +  ++W
Sbjct:   39 GVPQTPQPLTDAFLLRFLRARDFDLDLAWRLMKNYYKWRAE---CPEL--SADLRPRSILGLLKAGYHGVLRSRDSTGSRVLIYRIAYWDPKVFTAYDVFRVSLITSELIVQEVETQRNGVKAIFDL--------EGWQVSHAFQITPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKDRIHLHGNNYKSSMLQH--FPDILPREYGGKEFSMEDICQEW 259          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Clvs2 (clavesin 2 [Source:MGI Symbol;Acc:MGI:2443223])

HSP 1 Score: 90.1225 bits (222), Expect = 3.881e-19
Identity = 65/253 (25.69%), Postives = 121/253 (47.83%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTEN-GEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK-NPGLDKILPMEYGG 1807
            +G+S +T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF H  A + +  Y   R   + +   F +F   DP I   L+ G    L   ++ G  I ++    WD  +     ++  + ++++ +  D E Q++G  +IID  + + K       P  ++     +Q+S+P R  G  F N+P     ++ V+  F+ EK + R     N++N   +  +P   +ILP E+GG
Sbjct:    6 AGLSPETLEKARLELNENPDTLHQDIQEVRDMVITRPDIGFLRTDDAFILRFLRARKFHHFEAFRLLAQYFEYR---QQNLDMFKSFKATDPGIKQALKDGFPGGLANLDHYGRKILVLFAANWDQSRYTLVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLT-PNMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKEKTRKRIFLHGNNLNSLHQLIHP---EILPSEFGG 251          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Clvs1 (clavesin 1 [Source:MGI Symbol;Acc:MGI:1921688])

HSP 1 Score: 88.1965 bits (217), Expect = 3.010e-18
Identity = 67/253 (26.48%), Postives = 118/253 (46.64%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK-NPGLDKILPMEYGG 1807
            +G+S  T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF    A + +  Y   R   + +   F  F  +DP I   L  G    L  R   G  I L+    WD  +  F  ++  + ++++++  D E QI+G  +IID  + S K       P  +K     +Q+S+P R  G  F N+P     ++ ++  F+ +K + R     N++N   +  +P   + LP E+GG
Sbjct:   28 AGLSPDTIEKARLELNENPDILHQDIQQVRDMIITRPDIGFLRTDDAFILRFLRARKFHQADAFRLLAQYFQYR---QLNLDMFKNFKADDPGIKRALIDGFPGVLENRDHYGRKILLLFAANWDQSRNSFTDILRAILLSLEVLIEDPELQINGFILIIDWSNFSFKQASKLT-PSILKLAIEGLQDSFPARFGGVHFVNQPWYIHALYTLIKPFLKDKTRKRIFLHGNNLNSLHQLIHP---EFLPSEFGG 273          
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9BTX7|TTPAL_HUMAN (Alpha-tocopherol transfer protein-like OS=Homo sapiens OX=9606 GN=TTPAL PE=1 SV=2)

HSP 1 Score: 109.768 bits (273), Expect = 1.007e-24
Identity = 73/256 (28.52%), Postives = 125/256 (48.83%), Query Frame = 2
Query: 1061 GISEKTKQFAIDDIGENPSQVNAHIESFRRWL-KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
             ++E     A +++ E P      +++ R  + K  P+L    D  FL++FLR  KFD+ RA + + NY + R   R  P  FN  ++    +   L +G    L  T+  G  +  +R   W P   P  + I  +++ ++ +   EETQ++G+ ++ D K  S      +G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EK+ +R     +D+N      P    ILP EYGG  G +
Sbjct:   34 SLTEDLVTKAREELQEKPEWRLRDVQALRDMVRKEYPNLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHSCR---RSWPEVFN--NLKPSALKDVLASGFLTVLPHTDPRGCHVVCIRPDRWIPSNYPITENIRAIYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFG-PFIAKKVIGILQDGFPIRIKAVHVVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RSILPKEYGGTAGEL 281          
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5RFR0|TTPAL_PONAB (Alpha-tocopherol transfer protein-like OS=Pongo abelii OX=9601 GN=TTPAL PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.096e-24
Identity = 73/256 (28.52%), Postives = 125/256 (48.83%), Query Frame = 2
Query: 1061 GISEKTKQFAIDDIGENPSQVNAHIESFRRWL-KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
             ++E     A +++ E P      +++ R  + K  P+L    D  FL++FLR  KFD+ RA + + NY + R   R  P  FN  ++    +   L +G    L  T+  G  +  +R   W P   P  + I  +++ ++ +   EETQ++G+ ++ D K  S      +G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EK+ +R     +D+N      P    ILP EYGG  G +
Sbjct:   34 SLTEDLVTKAREELQEKPEWRLRDVQALRDMVRKEYPNLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHSCR---RSWPEVFN--NLKPSALKDVLASGFLTVLPHTDPRGCHVVCIRPDRWIPSNYPITENIRAIYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFG-PFIAKKVIGILQDGFPIRIKAVHVVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RSILPKEYGGTAGEL 281          
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9D3D0|TTPAL_MOUSE (Alpha-tocopherol transfer protein-like OS=Mus musculus OX=10090 GN=Ttpal PE=1 SV=3)

HSP 1 Score: 108.227 bits (269), Expect = 2.718e-24
Identity = 73/260 (28.08%), Postives = 125/260 (48.08%), Query Frame = 2
Query: 1061 GISEKTKQFAIDDIGENPSQVNAHIESFRRWL-KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECS 1834
             ++E     A +++ E P      +++ R  + K  P+L    D  FL++FLR  KFD+ RA + + NY   R   R  P  F+  ++    +   L +G    L  T+  G  +  +R   W P   P  + I  +++ ++ +   EETQ++G+ ++ D K  S      +G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EK+ +R     +D+N      P    ILP EYGG  G +   S
Sbjct:   35 SLTEDLVTKAREELQEKPEWRLRDVQALRDMVRKEYPYLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHGCR---RSWPEVFS--NLRPSALKDVLNSGFLTVLPHTDPRGCHVLCIRPDRWIPSNYPITENIRAVYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFG-PFIAKKVIGILQDGFPIRIKAVHIVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RNILPKEYGGTAGELDTAS 286          
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|P41034|TTPA_RAT (Alpha-tocopherol transfer protein OS=Rattus norvegicus OX=10116 GN=Ttpa PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.577e-21
Identity = 67/237 (28.27%), Postives = 110/237 (46.41%), Query Frame = 2
Query: 1157 KSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWG-------DPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKW 1843
            + +P    P    FL++FLR   FD   A + + NY   RA     P    +  ++   I   L+ G H  L  R   G  + + R   WDP+      +  +  +  ++I  + ETQ +G+K I DL        E W         P   K+I  V+ +S+PL+++G    NEPVIF  VF ++  F+ EK+K R     N+   ++ ++     ILP+EYGG   ++ +  ++W
Sbjct:   38 EGVPETPQPLTDAFLLRFLRARDFDLDLAWRLMKNYYKWRAE---CPEL--SADLHPRSILGLLKAGYHGVLRSRDPTGSRVLIYRISYWDPKVFTAYDVFRVSLITSELIVQEVETQRNGVKAIFDL--------EGWQISHAFQITPSVAKKIAAVVTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKGRIHLHGNNYKSSLLQH--FPDILPLEYGGNESSMEDICQEW 259          
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|P49638|TTPA_HUMAN (Alpha-tocopherol transfer protein OS=Homo sapiens OX=9606 GN=TTPA PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.086e-20
Identity = 67/235 (28.51%), Postives = 111/235 (47.23%), Query Frame = 2
Query: 1163 MPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWG-------DPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKW 1843
            +P    P    FL++FLR   FD   A + + NY   RA     P    +  ++   I   L+ G H  L  R   G  + + R   WDP+      +  +  +  ++I  + ETQ +G+K I DL        E W         P   K+I  V+ +S+PL+++G    NEPVIF  VF ++  F+ EK+K R     N+  +++ ++     ILP+EYGG+  ++ +  ++W
Sbjct:   40 VPLAPLPLTDSFLLRFLRARDFDLDLAWRLLKNYYKWRAE---CPEI--SADLHPRSIIGLLKAGYHGVLRSRDPTGSKVLIYRIAHWDPKVFTAYDVFRVSLITSELIVQEVETQRNGIKAIFDL--------EGWQFSHAFQITPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKERIHMHGNNYKQSLLQH--FPDILPLEYGGEEFSMEDICQEW 259          
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A5J4P3R6 (CRAL-TRIO domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0006189 PE=4 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 5.209e-77
Identity = 119/315 (37.78%), Postives = 191/315 (60.63%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFE 2002
            + +S+  +  A  ++GE    V AH+ SF+RWL S PHL+ P +  FL+ FLR + ++H  A++RIDN+CT+R S +    +++  ++ DP+++TYL  G+ +PLG  E+G  + L+R   W P +I   ++  L  M  + +  D+  QI G  ++ID+  +S +    WGD KY K   ++MQE+ P R+K  +FY E  IFD  F++   +M++KM+ R IR+K++I KA +K PGL +ILP EY G+ GT+  C  +     KEFY  G P+    VDE KRP  + +Y+ EY +++   +GT GT+ K +
Sbjct:    7 NSLSQFFRDLAKKEVGEKYDHVGAHLISFKRWLASSPHLKLPQNDVFLLSFLRYAHYEHALAQRRIDNFCTLRCSDKLISQWYDYPTVEDPLLDTYLNAGIFIPLGFLESGLYVVLIRMASWSPTEISQVEMRTLNNMNFERLLFDQRCQIGGFVLMIDMAHSSLETARQWGDIKYAKSAFKLMQEASPARVKHLVFYKESKIFDFAFKLSELWMSDKMRGRVIRVKDNIQKAFKKVPGLKEILPEEYEGKNGTLASCVIRCSREVKEFYSKGYPLKDIQVDEDKRPASARNYIKEYKEFDAPMMGTSGTFVKID 321          
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A4E0RHZ7 (Alpha-tocopherol transfer protein OS=Fasciola hepatica OX=6192 GN=D915_000833 PE=4 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 1.182e-75
Identity = 122/314 (38.85%), Postives = 194/314 (61.78%), Query Frame = 2
Query: 1064 ISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASP-RGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFE 2002
            + +  +Q A  ++ E+   + AHIESF+RW+ S+PH+  P D K L+ FLR +K+ H +A+ R+DN+CT+RAS   G P +F+   +NDPI+NTYL+ G+ + LGRT++  +I L+R   WDP+ +P   +  L +M ++    D  TQI G  +  D+   S + +    D   +K   +V QE  PLR+K  I+YNEP I D++F+++  ++++K++ R +R+K +I+KA +K PGL  ++P EYGG+ G +++  E    RF+EFY    P  G  VDESKRP  + +Y+ EY D      GT+GTY   +
Sbjct:    9 LPQNLRQKARKELHEDERHLKAHIESFQRWVDSLPHITYPKDPKLLLPFLRHAKYRHDKAQARLDNFCTLRASDLNGVPHWFHFPPVNDPIVNTYLKAGLVVELGRTKDDVLILLIRIGSWDPDILPMNLIRPLGYMNLERYVLDPVTQIAGYGLFFDMTGLSSRQLMHRKDKSEIKLEMKVFQEGLPLRVKHLIYYNEPAIVDVMFKLMDTWISDKIRQRMLRVKTNIDKAYDKVPGLKDLMPAEYGGKNGKLSDLIENHARRFREFYSKPPPWIGVRVDESKRPDSAKNYMREYKDVNIDIFGTKGTYISLD 322          
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A3Q0KS00 (Retinaldehyde binding protein-related OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 2.790e-74
Identity = 126/312 (40.38%), Postives = 185/312 (59.29%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRA-SPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTY 1990
            S +S    + A  ++ ENP+ + AH+ +F+RWL S PHL  P D  FL+ FLR + +DH  A+KRIDN+C++R  S  G   ++    + DPI++ YL+ G+ +PLG  ++G  IF++R K W P+ +   ++  L  M  +    DE  QI G+ + IDL   + K +  W DP+  K   +++QE+ P R K  IFY E   FDI F+V  F++++KMK R IRIK+D  KA +K PGL +I+P EYGG  G I +     K  FK FY     +S   VDESKRP  + +Y+ EY D E  T+G +GT+
Sbjct:    7 SNLSPFYVELAKKNLSENPAHIQAHLTAFKRWLSSCPHLTIPQDDAFLLGFLRYAHYDHSVAQKRIDNFCSIRCLSKHGITEWYKYPKLTDPIVDVYLDAGILVPLGIVDSGVHIFILRLKAWQPDLLSQTQMRALNNMNFERFILDERCQIGGVGIFIDLSGTTTKQISEWTDPRTAKSSMKLLQEATPGRTKHLIFYKESKAFDIGFKVFEFWLSDKMKQRIIRIKDDTEKAFKKIPGLKQIMPEEYGGCNGPIKDLITLDKRNFKTFYSQTCCLSVIKVDESKRPLSAQNYMREYKDMESTTMGNKGTF 318          
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A5K3FU18 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 4.261e-74
Identity = 120/312 (38.46%), Postives = 187/312 (59.94%), Query Frame = 2
Query: 1064 ISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNT-FSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTK 1996
            +S K  + A  ++GE+P  + AH+E+FRRWL SMPHL CP D  FL+ FLR SK+ H +A+ RIDN+CT+  +     +  +T   +    +  YL+ G+HLPLG  E+G+++F +R   W+P++I F +L   ++  + ++  D  T I G   I++  D + K +    DP  ++ + R  QE+ P RIK  ++YNE  + D    +  F+M EKMK+R I++  D+NKA E  PG+  ++P + GG+G  + E  ++ K  F EFY  G+P S   VDE+KRP  + +YL EY DY+   +GT GT+ K
Sbjct:    7 LSSKFLKLAKSELGEDPKNIPAHLEAFRRWLSSMPHLTCPDDDDFLLMFLRHSKYVHAKAQARIDNFCTLSTTKIIGDTVLSTPVDLESKDLKKYLDWGVHLPLGYAEDGKLVFYIRLGAWNPDEISFSQLFYYVYKVVFMVTIDPRTLIGGTIFILNFSDMTTKQIMR--DPNTIRTVVRFNQEAMPARIKRFVYYNEGKLLDTTIAIFKFYMKEKMKNRIIQVGTDMNKAFEAEPGIKALMPPDVGGEGRPLEEVIKENKKNFYEFYGKGDPTSKIKVDETKRPVSARAYLHEYKDYDPNVMGTSGTFIK 316          
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A1S8X0L3 (CRAL-TRIO domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=X801_04070 PE=4 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 5.122e-74
Identity = 117/308 (37.99%), Postives = 183/308 (59.42%), Query Frame = 2
Query: 1079 KQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFE 2002
            +  A  ++GEN   V AH+ SF+RWL + PHL+ P    FL+ FLR + +DH  A++RIDN+CT+R S +    ++    ++DP +  YL  G+ +PLG  +NG  + L+R   W P ++    +  +  M  + +  D+  QI G  + ID+   + +    WGD K+ K   ++MQES P R+K  IFY E  +FD  F++   +++EKM++R  R+K+DINKA +K PGL  +LPMEY G+ GT+ E   +     +EFY NGN +   +VDESKRP  + +Y+ EY + +   +GT GT+ K +
Sbjct:   70 QNLAKKEVGENAEHVMAHLTSFKRWLAASPHLKIPQVDTFLLSFLRYAHYDHALAQRRIDNFCTLRYSDKHISPWYEYPKLDDPRLEKYLNAGIFVPLGFMDNGMYVILIRLASWSPAELDQVAMRAMNNMNFERLLFDQRCQIGGFTLFIDMAHTTMEQATQWGDFKHAKSAFKLMQESSPGRVKNLIFYKETKLFDFAFKLAEVWLSEKMRNRVTRVKDDINKAFKKIPGLKDVLPMEYHGKNGTLEEALARCNRELREFYANGNVLQEIAVDESKRPSSARNYIREYKELDQSMMGTSGTFIKLD 377          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: ttpal (alpha tocopherol transfer protein like [Source:NCBI gene;Acc:103043466])

HSP 1 Score: 103.99 bits (258), Expect = 8.694e-24
Identity = 70/247 (28.34%), Postives = 118/247 (47.77%), Query Frame = 2
Query: 1088 AIDDIGENPSQVNAHIESFRRW-LKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
            A +++ E P      +++ R   LK  P+L+   D  FL++FLR  KFD+ RA + + NY     S R  P  F    +    +   L+ G    L R +  G  I  +R   W P    F   I  +++ ++ +   EETQ++G+ +++D          + G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EKM  R +   +D++      P    +LP EYGG  G +
Sbjct:   46 AREELQEKPEWRLRDVQALRDMILKEQPNLRTRLDDAFLLRFLRARKFDYDRAMQLLLNY---HGSRRAWPEVFQ--DLKPSTVKHVLDLGFLTVLPRPDPQGRYILCLRPGKWKPNDYHFVDNIRAIYLTLEKLIQPEETQVNGIVILVDYTGVGLSQASNPG-PLLAKKVVSILQDGFPIRIKAVNIINEPRIFKGIFAIIKPFLKEKMAERYVLHGSDLSSLHRVIP--RSVLPEEYGGVAGRL 284          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: ttpal (alpha tocopherol transfer protein like [Source:NCBI gene;Acc:103043466])

HSP 1 Score: 103.99 bits (258), Expect = 9.221e-24
Identity = 70/247 (28.34%), Postives = 118/247 (47.77%), Query Frame = 2
Query: 1088 AIDDIGENPSQVNAHIESFRRW-LKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTI 1822
            A +++ E P      +++ R   LK  P+L+   D  FL++FLR  KFD+ RA + + NY     S R  P  F    +    +   L+ G    L R +  G  I  +R   W P    F   I  +++ ++ +   EETQ++G+ +++D          + G P   K++  ++Q+ +P+RIK     NEP IF  +F ++  F+ EKM  R +   +D++      P    +LP EYGG  G +
Sbjct:   45 AREELQEKPEWRLRDVQALRDMILKEQPNLRTRLDDAFLLRFLRARKFDYDRAMQLLLNY---HGSRRAWPEVFQ--DLKPSTVKHVLDLGFLTVLPRPDPQGRYILCLRPGKWKPNDYHFVDNIRAIYLTLEKLIQPEETQVNGIVILVDYTGVGLSQASNPG-PLLAKKVVSILQDGFPIRIKAVNIINEPRIFKGIFAIIKPFLKEKMAERYVLHGSDLSSLHRVIP--RSVLPEEYGGVAGRL 283          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: ttpa (tocopherol (alpha) transfer protein [Source:ZFIN;Acc:ZDB-GENE-030131-4631])

HSP 1 Score: 89.7373 bits (221), Expect = 1.387e-18
Identity = 64/217 (29.49%), Postives = 104/217 (47.93%), Query Frame = 2
Query: 1196 FLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKW 1843
            FL+KFLR   FD   A K + NY   R       +     S+   + N Y     H  L  R + G  I + R   W+P++    ++  +  +  ++I  + +TQI+G+K I DL+     A     +P   K+I+ V+ +S+PL+++G    NEP+ F  VF+++  F+ +K+K R + I      A         I P EYGG G  + E  E+W
Sbjct:   48 FLLKFLRARDFDLDLALKLLINYHKWRQECPEISANLQPSSVIGLLKNNY-----HGVLKSRDDAGSRILIYRISQWNPKEFSAYEVFRVSLITSELIVQERDTQINGLKAIFDLQGWCF-AHAFQINPFLAKKISSVLTDSFPLKVRGIHLINEPIFFRPVFKMIRPFLPDKIKQR-VHIHGSSYTASLYEHFPKAIFPPEYGGTGPGLAEACEEW 257          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: clvs2 (clavesin 2 [Source:NCBI gene;Acc:103047212])

HSP 1 Score: 87.0409 bits (214), Expect = 3.658e-18
Identity = 63/252 (25.00%), Postives = 118/252 (46.83%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGG 1807
            +G+S +T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF+H  A + +  Y   R   + +   F      DP I   L+ G    L   + +G  I ++    WD  +  F  ++  + ++++ +  D E Q++G  +IID  + + K       P  ++     +Q+S+P R  G  F N+P     ++ V+  F+ +K + R     N++N        + +ILP E GG
Sbjct:    6 AGLSPETLEKAKVELKENPDTLHQDIQEVRDMIITRPDIGFLRTDDAFILRFLRARKFNHFEAFRLLAQYFEYR---QQNLDMFKNLKATDPGIKQALKDGFPGVLSNLDRHGRKILVLFAANWDQSRYTFVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLT-PSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKDKTRKRIFMHGNNLNSL--HQLIVPEILPSELGG 251          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: clvs2 (clavesin 2 [Source:NCBI gene;Acc:103047212])

HSP 1 Score: 87.0409 bits (214), Expect = 4.802e-18
Identity = 63/252 (25.00%), Postives = 118/252 (46.83%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGG 1807
            +G+S +T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF+H  A + +  Y   R   + +   F      DP I   L+ G    L   + +G  I ++    WD  +  F  ++  + ++++ +  D E Q++G  +IID  + + K       P  ++     +Q+S+P R  G  F N+P     ++ V+  F+ +K + R     N++N        + +ILP E GG
Sbjct:   23 AGLSPETLEKAKVELKENPDTLHQDIQEVRDMIITRPDIGFLRTDDAFILRFLRARKFNHFEAFRLLAQYFEYR---QQNLDMFKNLKATDPGIKQALKDGFPGVLSNLDRHGRKILVLFAANWDQSRYTFVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLT-PSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKDKTRKRIFMHGNNLNSL--HQLIVPEILPSELGG 268          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Match: ttpal (tocopherol (alpha) transfer protein-like [Source:ZFIN;Acc:ZDB-GENE-030131-296])

HSP 1 Score: 112.079 bits (279), Expect = 6.381e-28
Identity = 67/210 (31.90%), Postives = 106/210 (50.48%), Query Frame = 2
Query: 1097 DIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSR 1723
            ++GENP      ++  R  L   P +   TD  FL++FLR  KFDH++  K +++Y     +    P  F  F  +   +   L +G    L  R   G  + +     W+P   P    I  M+M M+++   EETQ++G+ ++ D K   R A  +   P  MK+I  VMQ+++P+RIKG    NEP IF  +F ++  F+ EKM+ R
Sbjct:   45 ELGENPETRAQEVQKLREALARRPDIPARTDDAFLLRFLRARKFDHEKTFKLVESYYKCHET---WPDIFQNFRPS--AVKALLGSGFIRVLPERDSKGRRVIIQSPGKWNPSTTPMMDNIRAMYMTMELLIQSEETQVNGITILADHKGV-RLAHVTNFTPSLMKKITTVMQDAFPIRIKGHNTVNEPSIFKAMFALMKPFLKEKMRKR 248          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003570.1 (pep scaffold:Pmarinus_7.0:GL486699:2092:8510:1 gene:ENSPMAG00000003262.1 transcript:ENSPMAT00000003570.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.9593 bits (206), Expect = 2.222e-18
Identity = 55/205 (26.83%), Postives = 98/205 (47.80%), Query Frame = 2
Query: 1040 TERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQ--ESYPLRIKGTI 1645
            TE      +S    + A  ++ E P      I++ R  LK  P +  PTD  FL++FLR  KFD+ +A   IDNY   R   R  P  F  F  +   +   LE G+   L + +  G  I ++R  +W+P   P   ++ +++M ++++   EETQ+ G+ ++ D            G P +++++  V+Q   + P+ + G +
Sbjct:   24 TEGTYVCRLSPHLVEVARQELQEKPEWRLRDIQALRDMLKEHPEIPAPTDDGFLLRFLRARKFDYDKALLLIDNYYKCR---RVWPDVFVNFYPSS--VRHVLEAGVVTALPQPDAQGRRIIVLRPGLWNPAVAPATDMLRVLYMTLEMLVRQEETQVCGLVVLADYAGLGLAHASHLG-PAFVRRVATVLQVGSTPPVSVSGRV 222          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007468.1 (pep scaffold:Pmarinus_7.0:GL477833:5549:9144:1 gene:ENSPMAG00000006747.1 transcript:ENSPMAT00000007468.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 71.633 bits (174), Expect = 4.167e-15
Identity = 36/116 (31.03%), Postives = 63/116 (54.31%), Query Frame = 2
Query: 1376 RTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSR 1723
            R   G  + +     W+P   P       M+M ++++   EETQ++G+ +++D K      + ++  P  MK+I  V+Q+++P+RIKG    NEP IF  +F ++  F  EKM+ R
Sbjct:   34 RDSKGRRVIIQSPGKWNPSTTPIIDNFKAMYMTLELLIQSEETQVNGITILVDHKGMGLAHVTNF-TPSLMKKITTVIQDAFPIRIKGHHTINEPSIFKAMFALIKPFFKEKMQKR 148          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Match: clvs2 (clavesin 2 [Source:NCBI gene;Acc:566769])

HSP 1 Score: 56.225 bits (134), Expect = 2.730e-9
Identity = 42/168 (25.00%), Postives = 74/168 (44.05%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTEN-GEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRK 1555
            SG+   T + A  ++ E P  +   I+  R  + + P +    TD  F+++FLR  KF H  A K +  Y   R         F  F   D  I   L  G    L  +++ G  I ++    WD  +  F  ++  + ++++ +  + E Q++G  +IID  + S K
Sbjct:    7 SGLRADTLEKARVELCERPDAIQQDIQEVRDMIVTRPDIGFLRTDDAFVLRFLRARKFHHFEAFKLLAQYFEYRQQ---HLDMFKNFKATDQGIRQALRNGFPGVLSHSDHYGRKILVIFASNWDQIRYTFVDILRAILLSLETMIEEPELQVNGFVLIIDWSNFSFK 171          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001440.1 (pep scaffold:Pmarinus_7.0:GL476337:1030926:1032713:1 gene:ENSPMAG00000001302.1 transcript:ENSPMAT00000001440.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 1.662e-7
Identity = 22/56 (39.29%), Postives = 31/56 (55.36%), Query Frame = 2
Query: 1097 DIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNY 1264
            ++GENP      ++  R  L   P +   TD  FL++FLR  KFDH++  K I NY
Sbjct:   16 ELGENPETRAQEVQKLREALARRPDIPARTDDAFLLRFLRARKFDHEKTLKLIKNY 71          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: ttpal (alpha tocopherol transfer protein like [Source:NCBI gene;Acc:101167039])

HSP 1 Score: 112.464 bits (280), Expect = 1.430e-26
Identity = 77/285 (27.02%), Postives = 134/285 (47.02%), Query Frame = 2
Query: 1088 AIDDIGENPSQVNAHIESFRRW-LKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTE-NGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGG---------TITECSEKWKIRFKEFYKNGNPVSGFSVDES 1909
            A +++ E P      +++ R   LK  P+L    D  FL++FLR  KFD+ RA + + NY    A  R  P  F    +    +   L+ G    L R + +G  I  +R   W P   PF   +  +++ ++ +   EETQ++G+ +++D          + G P   K++  ++Q+ +P+RIK     NEP+IF  +F ++  F+ EKM  R I   +D+       P    +LP EYGG  G          + +C E++ + F +     +P+ G  + +S
Sbjct:   44 AREELQEKPEWRLRDVQALRDMILKEQPNLHTRLDDAFLLRFLRARKFDYDRALQLLLNY---HAGRRAWPEVFQ--DLKPSTVKHVLDLGFLTVLPRPDPSGRYILCLRPGKWKPNDYPFVDNVRAIYLTLEKLIQPEETQVNGIIILVDYTGVGMSQASNPG-PFLAKKVVSILQDGFPIRIKAVNIINEPLIFKGIFAIIKPFLKEKMAERYILHGSDLRSLHRNLPA--SVLPEEYGGTAGRLDMGAWSRLLLDCEEEFIVEFCQ----PDPLEGVMLPDS 316          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: clvs2 (clavesin 2 [Source:NCBI gene;Acc:101159632])

HSP 1 Score: 87.4261 bits (215), Expect = 2.983e-18
Identity = 65/253 (25.69%), Postives = 119/253 (47.04%), Query Frame = 2
Query: 1058 SGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTEN-GEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK-NPGLDKILPMEYGG 1807
            +G+S +T + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KF+H  A + +  Y   R   + +   F      DP I   L+ G    L   +  G  I ++    WD  +  F  ++  + ++++ +  D E Q++G  +IID  + + K       P  ++     +Q+S+P R  G  F N+P     ++ V+  F+ +K + R     N++N   +  NP   +ILP E GG
Sbjct:    6 AGLSPETLEKAKVELKENPDTLHQDIQEVRDMIITRPDIGFLRTDDAFILRFLRARKFNHFEAFRLLAQYFEYR---QQNLDMFKNLKATDPGIKQALKDGFPGVLSNLDKYGRKILVLFAANWDQSRYTFVDILRAILLSLESMIEDPELQVNGFILIIDWSNFTFKQASKLT-PSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKDKTRKRIFMHGNNLNSLHQLINP---EILPSELGG 251          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: TTPA (alpha tocopherol transfer protein [Source:NCBI gene;Acc:101155881])

HSP 1 Score: 85.1149 bits (209), Expect = 8.125e-18
Identity = 72/236 (30.51%), Postives = 110/236 (46.61%), Query Frame = 2
Query: 1181 PTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVR-----TKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDAS-RKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK--NPGLDKILPMEYGGQGGTITECSEKWKIRFKEF 1864
            P    FL+KFLR   FD + + K + NY   R   R SP   +  S +  +   ++     LP  R   G  + + R     TK W P Q+       +  M  +II  + ETQ  G+K I DL+  S   A++   +P   K+I+ V+ +S+PL+++G    NEP  F  VF ++  F+ EK+K R      D +  +    +P    +LP EYGG G  I E  + W  +   F
Sbjct:   47 PLSDGFLLKFLRARDFDVELSLKLLLNYLRWR---RDSPEISSCLSPSSVLGLLHMSYHAVLP-QRDHAGSRVLIYRIGQWNTKDWSPYQV-----FRVSLMTSEIISMETETQRRGLKAIFDLQGWSLGHALQI--NPSLAKKISCVLSDSFPLKVRGIHLVNEPRFFRPVFAMIRPFLPEKIKQRIHMHGADFHSDLRHFFHP---HVLPPEYGGHGPGIDEACQDWTNQLLHF 268          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: TTPA (alpha tocopherol transfer protein [Source:NCBI gene;Acc:101155881])

HSP 1 Score: 85.5001 bits (210), Expect = 8.582e-18
Identity = 72/236 (30.51%), Postives = 110/236 (46.61%), Query Frame = 2
Query: 1181 PTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVR-----TKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDAS-RKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEK--NPGLDKILPMEYGGQGGTITECSEKWKIRFKEF 1864
            P    FL+KFLR   FD + + K + NY   R   R SP   +  S +  +   ++     LP  R   G  + + R     TK W P Q+       +  M  +II  + ETQ  G+K I DL+  S   A++   +P   K+I+ V+ +S+PL+++G    NEP  F  VF ++  F+ EK+K R      D +  +    +P    +LP EYGG G  I E  + W  +   F
Sbjct:   50 PLSDGFLLKFLRARDFDVELSLKLLLNYLRWR---RDSPEISSCLSPSSVLGLLHMSYHAVLP-QRDHAGSRVLIYRIGQWNTKDWSPYQV-----FRVSLMTSEIISMETETQRRGLKAIFDLQGWSLGHALQI--NPSLAKKISCVLSDSFPLKVRGIHLVNEPRFFRPVFAMIRPFLPEKIKQRIHMHGADFHSDLRHFFHP---HVLPPEYGGHGPGIDEACQDWTNQLLHF 271          
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: CLVS1 (clavesin-1 [Source:NCBI gene;Acc:101158238])

HSP 1 Score: 83.5741 bits (205), Expect = 4.588e-17
Identity = 62/253 (24.51%), Postives = 117/253 (46.25%), Query Frame = 2
Query: 1055 YSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQ-CPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPL-GRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGG 1807
            +S +S +  + A  ++ ENP  ++  I+  R  + + P +    TD  F+++FLR  KFD     + +  Y   R     +   F +F ++D  I   L  G    L    ++G  I ++    WD  +  F  ++  + ++++++  + E QI+G  +IID  + S K       P  +K     +Q+S+P R  G  F N+P     ++ ++  F+ +K + R     N++N        L + LP E+GG
Sbjct:    5 HSSLSSEMMEKARLELNENPDTLHQDIQQVRDMIVTRPDIGFLRTDDDFILRFLRARKFDQLETFRLLAQYFQFRQQ---NLDMFQSFKVDDLGIKRALMDGFPGVLESPDQHGRKILILFASNWDQSRNSFIDILRAILLSLEVLIENPELQINGFILIIDWSNFSFKQASKLT-PNILKLAIEGLQDSFPARFGGIHFVNQPWYIHAMYTIIKPFLKDKTRKRIFLHGNNLNSL--HQLILPECLPSEFGG 251          
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009876.1 (SMESG000009876.1)

HSP 1 Score: 677.552 bits (1747), Expect = 0.000e+0
Identity = 324/325 (99.69%), Postives = 324/325 (99.69%), Query Frame = 2
Query: 1037 MTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFENVF 2011
            MTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGE IFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFENVF
Sbjct:    1 MTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGETIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFENVF 325          
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009874.1 (SMESG000009874.1)

HSP 1 Score: 464.922 bits (1195), Expect = 3.954e-160
Identity = 206/325 (63.38%), Postives = 270/325 (83.08%), Query Frame = 2
Query: 1037 MTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFENVF 2011
            M +RYEY+ ISE++KQFAIDD+GENPSQVNAHIESFRRW+KSMPHL CPTD++FL+KFLRVSKFDHK+ +KR+DN+CTVR+SPRGSP FF+ F+++DPI++ YL++ + LPLGR + GE+I L++ + WD E+IP  + + L FM +DI+ ++EETQIHG+ ++IDLKD SRK++E+  +PK +KQ N  +Q+SYP+R+K  IF NEPVIFD+VFQ+ SFFMN+KM+ RT RIKNDINKA EK PG+  +LP EY G+GGT+  C+EKWK RFK +YKNG+P+ G SV+E+KRP+E+ASYL  Y DY G TLGT GT+TKFENV 
Sbjct:    1 MQDRYEYNRISERSKQFAIDDLGENPSQVNAHIESFRRWVKSMPHLICPTDSEFLLKFLRVSKFDHKKTQKRLDNFCTVRSSPRGSPDFFHYFNVDDPIVDMYLDSKVQLPLGRNDAGEMILLMKPEGWDSEKIPVNQFLSLFFMGLDILLSEEETQIHGLTLLIDLKDVSRKSIEAMAEPKILKQYNMAVQDSYPIRMKKQIFCNEPVIFDVVFQIYSFFMNDKMRGRTHRIKNDINKAFEKVPGIKTVLPTEYNGEGGTLEYCAEKWKHRFKTYYKNGDPLKGISVEEAKRPKETASYLVAYKDYVGPTLGTHGTFTKFENVL 325          
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009872.1 (SMESG000009872.1)

HSP 1 Score: 463.381 bits (1191), Expect = 1.423e-159
Identity = 198/325 (60.92%), Postives = 268/325 (82.46%), Query Frame = 2
Query: 1037 MTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFENVF 2011
            M +RYEYSG+SE +K+ A++ + ENP QVNAHIES RRW+KSMPH+ CPTD  FL+KFLRV+KFDH +AE+R+DN+CT R+S +GS  FF  F I D ++ T+L + MH+PLG+ + GE+IFL+R+ VWDPE+IP  KLI+L+FM +DII N EE+QIHG++M+IDL D SRK++ESW +PK +KQ ++V+QESYPLR+KG IFYNEPV+FD++++++S FMN+KMK RT R+KNDI KA+EK PGL+KILP EY GQGG++ EC+EKWK +F+ +YKNGNP    SVDESKRP+ +++YL ++ DY+ +T GT G++TKFENVF
Sbjct:    1 MNDRYEYSGVSENSKKAAVEYLNENPEQVNAHIESLRRWVKSMPHINCPTDGNFLLKFLRVAKFDHDKAERRLDNFCTARSSKKGSEEFFKFFKIEDQVVQTFLSSKMHIPLGQNDAGEIIFLIRSNVWDPEKIPMVKLIVLIFMGLDIIINMEESQIHGIRMLIDLNDISRKSLESWAEPKLLKQWHKVVQESYPLRVKGQIFYNEPVLFDLIYELISLFMNDKMKERTFRVKNDIKKAMEKIPGLEKILPTEYNGQGGSLNECAEKWKNKFESYYKNGNPFECISVDESKRPKANSNYLTDHSDYQNKTFGTSGSFTKFENVF 325          
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009988.1 (SMESG000009988.1)

HSP 1 Score: 353.984 bits (907), Expect = 6.751e-117
Identity = 153/322 (47.52%), Postives = 224/322 (69.57%), Query Frame = 2
Query: 1037 MTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTLGTQGTYTKFE 2002
            M + Y Y G++++ KQ A+ ++ E+P QVNAHIESFRRW+KSMPHL CPTD++FL+ FLRV+KFDH +A+KR+DN+CTVR+S RG P +F    IND +   + +T   +PLGRT +GE +F   + +W+P++IP  KL+ +MFM++++   ++  QI+GMK  +D+    +  ME+  DPK MK + RV Q+ YP+R KG + Y EP  + + + ++ FF+NEK + R  RIK DI K  EK PGL+ +LP EYGG+G  + E  +KW    ++FY  G+P +  SVDESKRP E+  Y  +Y DY G++LGT+GT+TKF+
Sbjct:    1 MADTYTYDGLTDRDKQVALTELNEDPKQVNAHIESFRRWVKSMPHLNCPTDSRFLLMFLRVAKFDHAKAQKRLDNFCTVRSSARGHPEYFQFPDINDAVFEKFFQTKQMIPLGRTVDGETLFFGVSNLWNPKEIPIKKLMQVMFMSLEVQLQNQYLQIYGMKAFMDMHGCGKSFMEAVSDPKVMKAMTRVWQDCYPIRFKGQVIYREPGWYSVFYNIMEFFLNEKHRKRVFRIKEDIQKGFEKIPGLEALLPEEYGGKGPKMDEAIDKWAKEVRDFYTKGSPWANISVDESKRPVETLKYFEQYADYTGESLGTKGTFTKFD 322          
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009877.1 (SMESG000009877.1)

HSP 1 Score: 311.997 bits (798), Expect = 1.511e-100
Identity = 142/326 (43.56%), Postives = 219/326 (67.18%), Query Frame = 2
Query: 1037 MTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASYLAEYPDYEGQTL-GTQGTYTKFENVF 2011
            M + Y+Y  +S +  +   ++I E+ +QVNAHIESFRRW+ SMPHL+CPTD+KFL+ FLR +KFDH  A++R+DN+CT+R+SPRGSP FF   S+ + II   L +  ++P+G+   GE++ L +T  W PE+I    ++ +++M  D+I   E TQI+G+  I DL+   RK +E+W DP   K I R+ Q+++P+R+KG I Y  P +F I+ +++ FFM +K K R ++I +  +K   K  GL +I+P EYGG GG + +  E W    + FY  G+P S  SVDE+KRP ++ S+L  Y +Y+G+T+ GT G+YTK+E+++
Sbjct:    1 MADVYKYETLSVRDSEILKNEIKEDKNQVNAHIESFRRWVVSMPHLKCPTDSKFLLMFLRTAKFDHSVAQRRLDNFCTIRSSPRGSPRFFKFPSLKNSIIEKVLRSHQNIPMGKDSAGEMVLLFKTGDWKPEEISVENILQVVYMTFDLILTRESTQINGIVSIFDLEGCCRKTLEAWSDPHLTKNIMRIWQDAFPIRMKGIILYKPPTLFHIILKIMEFFMIDKHKKRILQIDSQ-DKLFSKVSGLKEIMPTEYGGNGGKLKDKVESWIGEVETFYDKGDPWSFISVDENKRPGDTVSFLRNYEEYKGETIGGTPGSYTKYEDLY 325          
The following BLAST results are available for this feature:
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TTPAL2.097e-2528.52alpha tocopherol transfer protein like [Source:HGN... [more]
TTPAL2.097e-2528.52alpha tocopherol transfer protein like [Source:HGN... [more]
TTPA2.262e-2128.51alpha tocopherol transfer protein [Source:HGNC Sym... [more]
CLVS26.437e-1925.69clavesin 2 [Source:HGNC Symbol;Acc:HGNC:23046][more]
CLVS12.080e-1826.48clavesin 1 [Source:HGNC Symbol;Acc:HGNC:23139][more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG339662.111e-2926.03gene:FBgn0053966 transcript:FBtr0100007[more]
CG335141.421e-2827.19gene:FBgn0053514 transcript:FBtr0091456[more]
CG129263.921e-2526.30gene:FBgn0033437 transcript:FBtr0088502[more]
CG316361.046e-2426.28gene:FBgn0051636 transcript:FBtr0079324[more]
CG316361.046e-2426.28gene:FBgn0051636 transcript:FBtr0343865[more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal2.114e-2530.36tocopherol (alpha) transfer protein-like [Source:Z... [more]
ttpal3.549e-2529.15tocopherol (alpha) transfer protein-like [Source:Z... [more]
clvs22.155e-1825.40clavesin 2 [Source:NCBI gene;Acc:566769][more]
ttpa9.909e-1727.06tocopherol (alpha) transfer protein [Source:ZFIN;A... [more]
rlbp1a2.475e-1424.05retinaldehyde binding protein 1a [Source:ZFIN;Acc:... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal1.485e-2529.84tocopherol (alpha) transfer protein-like [Source:N... [more]
ggh6.228e-2129.95gamma-glutamyl hydrolase (conjugase, folylpolygamm... [more]
thg1l8.781e-1925.69tRNA-histidine guanylyltransferase 1-like [Source:... [more]
clvs11.309e-1825.57clavesin 1 [Source:NCBI gene;Acc:100037913][more]
clvs13.671e-1825.20clavesin 1 [Source:NCBI gene;Acc:100037913][more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ttpal3.886e-2528.08tocopherol (alpha) transfer protein-like [Source:M... [more]
Ttpal3.886e-2528.08tocopherol (alpha) transfer protein-like [Source:M... [more]
Ttpa8.585e-2128.39tocopherol (alpha) transfer protein [Source:MGI Sy... [more]
Clvs23.881e-1925.69clavesin 2 [Source:MGI Symbol;Acc:MGI:2443223][more]
Clvs13.010e-1826.48clavesin 1 [Source:MGI Symbol;Acc:MGI:1921688][more]
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BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9BTX7|TTPAL_HUMAN1.007e-2428.52Alpha-tocopherol transfer protein-like OS=Homo sap... [more]
sp|Q5RFR0|TTPAL_PONAB1.096e-2428.52Alpha-tocopherol transfer protein-like OS=Pongo ab... [more]
sp|Q9D3D0|TTPAL_MOUSE2.718e-2428.08Alpha-tocopherol transfer protein-like OS=Mus musc... [more]
sp|P41034|TTPA_RAT8.577e-2128.27Alpha-tocopherol transfer protein OS=Rattus norveg... [more]
sp|P49638|TTPA_HUMAN1.086e-2028.51Alpha-tocopherol transfer protein OS=Homo sapiens ... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4P3R65.209e-7737.78CRAL-TRIO domain-containing protein OS=Paragonimus... [more]
A0A4E0RHZ71.182e-7538.85Alpha-tocopherol transfer protein OS=Fasciola hepa... [more]
A0A3Q0KS002.790e-7440.38Retinaldehyde binding protein-related OS=Schistoso... [more]
A0A5K3FU184.261e-7438.46Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A1S8X0L35.122e-7437.99CRAL-TRIO domain-containing protein OS=Opisthorchi... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal8.694e-2428.34alpha tocopherol transfer protein like [Source:NCB... [more]
ttpal9.221e-2428.34alpha tocopherol transfer protein like [Source:NCB... [more]
ttpa1.387e-1829.49tocopherol (alpha) transfer protein [Source:ZFIN;A... [more]
clvs23.658e-1825.00clavesin 2 [Source:NCBI gene;Acc:103047212][more]
clvs24.802e-1825.00clavesin 2 [Source:NCBI gene;Acc:103047212][more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal6.381e-2831.90tocopherol (alpha) transfer protein-like [Source:Z... [more]
ENSPMAT00000003570.12.222e-1826.83pep scaffold:Pmarinus_7.0:GL486699:2092:8510:1 gen... [more]
ENSPMAT00000007468.14.167e-1531.03pep scaffold:Pmarinus_7.0:GL477833:5549:9144:1 gen... [more]
clvs22.730e-925.00clavesin 2 [Source:NCBI gene;Acc:566769][more]
ENSPMAT00000001440.11.662e-739.29pep scaffold:Pmarinus_7.0:GL476337:1030926:1032713... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal1.430e-2627.02alpha tocopherol transfer protein like [Source:NCB... [more]
clvs22.983e-1825.69clavesin 2 [Source:NCBI gene;Acc:101159632][more]
TTPA8.125e-1830.51alpha tocopherol transfer protein [Source:NCBI gen... [more]
TTPA8.582e-1830.51alpha tocopherol transfer protein [Source:NCBI gen... [more]
CLVS14.588e-1724.51clavesin-1 [Source:NCBI gene;Acc:101158238][more]
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BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009876.10.000e+099.69SMESG000009876.1[more]
SMESG000009874.13.954e-16063.38SMESG000009874.1[more]
SMESG000009872.11.423e-15960.92SMESG000009872.1[more]
SMESG000009988.16.751e-11747.52SMESG000009988.1[more]
SMESG000009877.11.511e-10043.56SMESG000009877.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016902 ID=SMED30016902|Name=CRAL-TRIO domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2099bp
TTTGTTTTTAGATCAAAGGTGATCAAAAGAATAAATATTTTTACTGATTA
TATTCTATTGTTCTCATTAATGAAATAATCTCATTTCATCTACTGTCAAC
TAAAAATTCGCCAAAATATAAAAGTGGAAGATAAGCGAATTTAATTGATA
GGATTTTCTCTTACTTTTGTAAAGTATGTAGCATCCTGTTCTTGTGTTTA
TACTTCTTATTGCTCTTCTCTCTGCTTTATCTGTTTTAGAAAATCTATTT
CTCGGTTTTTACTAAAGTCTCTTACTGTAAAAATCATTGAGGCCTTTTAC
TTTTTTATCATCGTAACAATTTTCCTCATCCGATTTTTATTCTTTATTTA
AAACCAATTTCTCGAAAACTTAATCAAAGTTTTAATGTTGTCATTTTAGG
AATTTTGCATAAAAGTAGTAGTAATTTCTAAATATTCAAATTAGTCTTTG
AAAGGTAATTCTTTCCTCACTGTTCAGTTTCCGTTATGACTTGGTCAACA
CAACCTTTATTAGTGCCCTCTCTGTCCCATGTCACCCTGACTGACCCTAT
GCAAATGCAGACTCTACATGATCCGTTATAAAAACTGACATCAACTCATA
AATTTATTTTAAATTCGTAGTCAATCTGAAGCCCTGAAATAATACAACTT
TACTGTTTGTTTAACATTCAAACTAAAAAAAATATTTTTGAATTTTTATA
AAGTGATCACCACTAACCAAACCAGACGATTCTCACAGTTTCATAAAGGT
TTCATAAGCCTACCATTCTAACAGCAGTTACACTATTTTTGTGAAAAGTA
CATAAATTGGCTAAAGCTACAAATTCAATTGAATCGTCACTATTAAGCCA
TAATAACATCATAATTGTCATAATTATATGGTGCATACATCAAAAATCAG
AGCTACTGACTGCAGGGGCCCTTGAAGCATTCATATTTACTGAATAAATT
AGTTCAACCGATATTTGAAGACAGTTATAAGTTATAATTTATAGTCGTTC
TGTTTTTTTTTTCAATAAAACTATATAAGCATCAAAATGACAGAAAGGTA
TGAATATTCCGGAATTTCCGAAAAAACAAAACAGTTTGCCATAGATGATA
TCGGTGAAAATCCTTCTCAAGTCAATGCTCACATTGAAAGCTTTCGAAGA
TGGCTAAAGTCTATGCCTCACCTCCAGTGTCCAACAGATGCGAAGTTTTT
GATGAAGTTCTTACGAGTGTCAAAATTTGATCACAAAAGAGCAGAAAAGC
GAATAGATAACTATTGCACAGTAAGAGCTTCACCTAGAGGATCTCCGAGT
TTTTTCAACACATTCAGCATAAATGATCCGATCATAAACACATATTTGGA
AACCGGAATGCATCTTCCTTTAGGCAGGACGGAAAACGGTGAAGTAATTT
TCCTCGTGAGAACAAAAGTCTGGGATCCTGAACAAATTCCATTTCCGAAA
TTAATTATTTTGATGTTTATGGCAATGGATATCATTTGTAATGATGAAGA
GACTCAAATCCACGGCATGAAAATGATTATAGATCTCAAGGACGCGTCGA
GGAAAGCCATGGAAAGTTGGGGTGACCCAAAATACATGAAACAAATTAAC
CGAGTCATGCAGGAATCTTACCCACTTCGAATTAAAGGAACAATTTTCTA
CAACGAGCCCGTCATTTTTGATATTGTTTTTCAAGTGTTGTCGTTTTTCA
TGAATGAGAAAATGAAAAGTCGGACAATACGTATAAAAAATGACATAAAC
AAAGCAATCGAAAAGAATCCAGGGCTAGATAAAATTCTTCCTATGGAGTA
CGGCGGCCAAGGTGGAACTATCACAGAATGTTCCGAGAAATGGAAAATAA
GATTCAAGGAATTTTACAAAAACGGAAATCCCGTTAGTGGGTTTTCTGTT
GATGAGTCCAAGCGCCCCCAGGAATCGGCATCGTATTTGGCCGAATATCC
AGATTACGAAGGCCAAACTTTAGGAACGCAGGGGACCTACACGAAATTCG
AAAATGTTTTCTGATATCATAATTATTTATTAGGACATTTGTGACTTTTA
TCTGAATTTTATTAGAATTCAGCTTTTATTTTTCGAAAAAAAAGATCGG
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protein sequence of SMED30016902-orf-1

>SMED30016902-orf-1 ID=SMED30016902-orf-1|Name=SMED30016902-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=326bp
MTERYEYSGISEKTKQFAIDDIGENPSQVNAHIESFRRWLKSMPHLQCPT
DAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPII
NTYLETGMHLPLGRTENGEVIFLVRTKVWDPEQIPFPKLIILMFMAMDII
CNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIK
GTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKI
LPMEYGGQGGTITECSEKWKIRFKEFYKNGNPVSGFSVDESKRPQESASY
LAEYPDYEGQTLGTQGTYTKFENVF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036865CRAL-TRIO_dom_sf
IPR036273CRAL/TRIO_N_dom_sf
IPR001071CRAL-bd_toc_tran
IPR011074CRAL/TRIO_N_dom
IPR001251CRAL-TRIO_dom
Vocabulary: biological process
TermDefinition
GO:0006810transport
Vocabulary: molecular function
TermDefinition
GO:0005215transporter activity
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00180CRETINALDHBPcoord: 249..258
score: 60.0
coord: 54..76
score: 39.13
coord: 182..203
score: 27.27
NoneNo IPR availableGENE3DG3DSA:1.10.8.20coord: 4..82
e-value: 1.4E-10
score: 43.1
NoneNo IPR availableGENE3DG3DSA:1.20.5.1200coord: 102..273
e-value: 2.2E-38
score: 133.3
NoneNo IPR availablePANTHERPTHR10174RETINALDEHYDE BINDING PROTEIN-RELATEDcoord: 11..276
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 103..260
e-value: 2.0E-10
score: 50.6
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 103..258
e-value: 1.0E-21
score: 77.3
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 96..263
score: 13.625
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 110..258
e-value: 9.30342E-20
score: 82.433
IPR011074CRAL/TRIO, N-terminal domainSMARTSM01100CRAL_TRIO_N_2coord: 51..76
e-value: 8.8E-4
score: 28.6
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3DG3DSA:3.40.525.10coord: 104..251
e-value: 2.2E-38
score: 133.3
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILYSSF52087CRAL/TRIO domaincoord: 103..277
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILYSSF46938CRAL/TRIO N-terminal domaincoord: 8..81