SMED30016895

Overview
NameSMED30016895
Smed IDSMED30016895
Length (bp)505
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016895 (SMED30016895) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016895 (SMED30016895) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016895 (SMED30016895) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016895 (SMED30016895) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30016895

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30016895SMESG000032406.1 SMESG000032404.1 dd_Smed_v4_4715_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016895SMESG000032406.1 SMESG000032404.1 dd_Smed_v4_4715_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016895SMESG000032406.1 SMESG000032404.1 dd_Smed_v4_4715_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30016895SMESG000032406.1 SMESG000032404.1 dd_Smed_v6_4715_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016895SMESG000032406.1 SMESG000032404.1 dd_Smed_v4_4715_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016895 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006893:1320..11377 -2429
Homology
[Close]
BLAST of SMED30016895 vs. Ensembl Human
Match: NOSTRIN (nitric oxide synthase trafficking [Source:HGNC Symbol;Acc:HGNC:20203])

HSP 1 Score: 55.8398 bits (133), Expect = 4.848e-9
Identity = 40/154 (25.97%), Postives = 80/154 (51.95%), Query Frame = -2 
Query:   31 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGS-ICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSKVV 486
            NG+ + ++V  V ++R  LE+ YA  L K+ +++ KA Q ++ S + + W      M S +D+H++LG  ++ E  K      +   K +K ++ E+EK   ++     ++++ KKK   + K  E  ++ +E  K       S  +KE+ K++
Sbjct:   23 NGENFCKQVTSVLQQRANLEISYAKGLQKLASKLSKALQNTRKSCVSSAWAWASEGMKSTADLHQKLGKAIELEAIKPTYQVLNVQEKKRKSLDNEVEKTANLVISNWNQQIKAKKKLMVSTKKHEALFQLVESSKQ------SMTEKEKRKLL 170
[Close]
BLAST of SMED30016895 vs. Ensembl Human
Match: NOSTRIN (nitric oxide synthase trafficking [Source:HGNC Symbol;Acc:HGNC:20203])

HSP 1 Score: 55.4546 bits (132), Expect = 5.788e-9
Identity = 40/154 (25.97%), Postives = 80/154 (51.95%), Query Frame = -2 
Query:   31 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGS-ICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSKVV 486
            NG+ + ++V  V ++R  LE+ YA  L K+ +++ KA Q ++ S + + W      M S +D+H++LG  ++ E  K      +   K +K ++ E+EK   ++     ++++ KKK   + K  E  ++ +E  K       S  +KE+ K++
Sbjct:   23 NGENFCKQVTSVLQQRANLEISYAKGLQKLASKLSKALQNTRKSCVSSAWAWASEGMKSTADLHQKLGKAIELEAIKPTYQVLNVQEKKRKSLDNEVEKTANLVISNWNQQIKAKKKLMVSTKKHEALFQLVESSKQ------SMTEKEKRKLL 170
[Close]
BLAST of SMED30016895 vs. Ensembl Human
Match: NOSTRIN (nitric oxide synthase trafficking [Source:HGNC Symbol;Acc:HGNC:20203])

HSP 1 Score: 55.4546 bits (132), Expect = 5.788e-9
Identity = 40/154 (25.97%), Postives = 80/154 (51.95%), Query Frame = -2 
Query:   31 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGS-ICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSKVV 486
            NG+ + ++V  V ++R  LE+ YA  L K+ +++ KA Q ++ S + + W      M S +D+H++LG  ++ E  K      +   K +K ++ E+EK   ++     ++++ KKK   + K  E  ++ +E  K       S  +KE+ K++
Sbjct:   23 NGENFCKQVTSVLQQRANLEISYAKGLQKLASKLSKALQNTRKSCVSSAWAWASEGMKSTADLHQKLGKAIELEAIKPTYQVLNVQEKKRKSLDNEVEKTANLVISNWNQQIKAKKKLMVSTKKHEALFQLVESSKQ------SMTEKEKRKLL 170
[Close]
BLAST of SMED30016895 vs. Ensembl Fly
Match: Nost (gene:FBgn0259734 transcript:FBtr0343357)

HSP 1 Score: 88.1965 bits (217), Expect = 1.367e-20
Identity = 46/140 (32.86%), Postives = 80/140 (57.14%), Query Frame = -2 
Query:   85 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYK 504
            R+++K  GD  +E++ V +ER + EL Y+  LSK+ N++ KA +   GS+   W      M S SD+H++L   L DE  K L I  +   K +K VE+ ++K  ++L + R  E + KK + +A ++ EK  +++ D +
Sbjct:  673 RRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVR 812
[Close]
BLAST of SMED30016895 vs. Ensembl Fly
Match: Nost (gene:FBgn0259734 transcript:FBtr0302907)

HSP 1 Score: 88.1965 bits (217), Expect = 1.438e-20
Identity = 46/140 (32.86%), Postives = 80/140 (57.14%), Query Frame = -2 
Query:   85 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYK 504
            R+++K  GD  +E++ V +ER + EL Y+  LSK+ N++ KA +   GS+   W      M S SD+H++L   L DE  K L I  +   K +K VE+ ++K  ++L + R  E + KK + +A ++ EK  +++ D +
Sbjct:  568 RRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVR 707
[Close]
BLAST of SMED30016895 vs. Ensembl Fly
Match: Nost (gene:FBgn0259734 transcript:FBtr0300000)

HSP 1 Score: 88.1965 bits (217), Expect = 1.438e-20
Identity = 46/140 (32.86%), Postives = 80/140 (57.14%), Query Frame = -2 
Query:   85 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYK 504
            R+++K  GD  +E++ V +ER + EL Y+  LSK+ N++ KA +   GS+   W      M S SD+H++L   L DE  K L I  +   K +K VE+ ++K  ++L + R  E + KK + +A ++ EK  +++ D +
Sbjct:  568 RRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVR 707
[Close]
BLAST of SMED30016895 vs. Ensembl Fly
Match: Nost (gene:FBgn0259734 transcript:FBtr0302906)

HSP 1 Score: 88.1965 bits (217), Expect = 1.462e-20
Identity = 47/148 (31.76%), Postives = 83/148 (56.08%), Query Frame = -2 
Query:   61 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVS 504
            R+++K  GD  +E++ V +ER + EL Y+  LSK+ N++ KA +   GS+   W      M S SD+H++L   L DE  K L I  +   K +K VE+ ++K  ++L + R  E + KK + +A ++ EK  +++ D +     S++
Sbjct:   18 RRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVRIQKSPSIA 165
[Close]
BLAST of SMED30016895 vs. Ensembl Fly
Match: Nost (gene:FBgn0259734 transcript:FBtr0300001)

HSP 1 Score: 88.1965 bits (217), Expect = 1.474e-20
Identity = 47/148 (31.76%), Postives = 83/148 (56.08%), Query Frame = -2 
Query:   61 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVS 504
            R+++K  GD  +E++ V +ER + EL Y+  LSK+ N++ KA +   GS+   W      M S SD+H++L   L DE  K L I  +   K +K VE+ ++K  ++L + R  E + KK + +A ++ EK  +++ D +     S++
Sbjct:   14 RRYVKQGGDFSKELIFVLQERADSELIYSKSLSKLANKLNKAGREIPGSVADAWRGVATEMESRSDIHRQLAASLTDELVKPLKIVVEGHHKARKAVESNVDKAARVLGEWRASEAKAKKASHTAARENEKLQDAMLDVRIQKSPSIA 161
[Close]
BLAST of SMED30016895 vs. Ensembl Zebrafish
Match: nostrin (nitric oxide synthase trafficking [Source:ZFIN;Acc:ZDB-GENE-070522-2])

HSP 1 Score: 61.2326 bits (147), Expect = 3.885e-11
Identity = 38/136 (27.94%), Postives = 71/136 (52.21%), Query Frame = -2 
Query:   85 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAF-QPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYK 486
            NG+ + +E+L V ++R ELE+ Y+  L KI  +++K   + S  S    W+     M + +D H+ LGN L  +  + +    D+  K K+P++  +EK  K++     E+++ KKK     ++ E  +  +E  K
Sbjct:   23 NGEFFCKELLAVFQQRSELEINYSKGLQKIAGKLLKVSKEMSDNSTYHAWSFISDEMFASADAHRILGNALNQDVIQEIRQILDEHGKRKRPLDNAVEKSGKLVLTNWSEQIKLKKKLIGLTREHEALFSFVEKNK 158
[Close]
BLAST of SMED30016895 vs. Ensembl Zebrafish
Match: nostrin (nitric oxide synthase trafficking [Source:ZFIN;Acc:ZDB-GENE-070522-2])

HSP 1 Score: 61.2326 bits (147), Expect = 3.885e-11
Identity = 38/136 (27.94%), Postives = 71/136 (52.21%), Query Frame = -2 
Query:   85 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAF-QPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYK 486
            NG+ + +E+L V ++R ELE+ Y+  L KI  +++K   + S  S    W+     M + +D H+ LGN L  +  + +    D+  K K+P++  +EK  K++     E+++ KKK     ++ E  +  +E  K
Sbjct:   23 NGEFFCKELLAVFQQRSELEINYSKGLQKIAGKLLKVSKEMSDNSTYHAWSFISDEMFASADAHRILGNALNQDVIQEIRQILDEHGKRKRPLDNAVEKSGKLVLTNWSEQIKLKKKLIGLTREHEALFSFVEKNK 158
[Close]
BLAST of SMED30016895 vs. Ensembl Xenopus
Match: prag1 (PEAK1 related, kinase-activating pseudokinase 1 [Source:Xenbase;Acc:XB-GENE-5933625])

HSP 1 Score: 54.6842 bits (130), Expect = 9.792e-9
Identity = 37/134 (27.61%), Postives = 70/134 (52.24%), Query Frame = -2 
Query:   91 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQP-SKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIED 486
            NG+++ + ++   ++R  LEL YA  L K+ N++ KA    +K  I   WT     M + ++VHK+LG  +Q E  K      ++  K KK ++ E+EK    + +  +++++ KKK     K  E  +  +++
Sbjct:   23 NGEIFCKTLMSALQQRSNLELSYAKGLEKVANKLTKALASVNKNCINNAWTCASDEMRTTAEVHKKLGTAIQQEAIKPTSQVLEEHEKKKKKLDNEVEKMTSSVLNNWKQQIKVKKKLMEHTKKHEALFHHVDN 156
[Close]
BLAST of SMED30016895 vs. Ensembl Xenopus
Match: prag1 (PEAK1 related, kinase-activating pseudokinase 1 [Source:Xenbase;Acc:XB-GENE-5933625])

HSP 1 Score: 54.6842 bits (130), Expect = 1.052e-8
Identity = 37/134 (27.61%), Postives = 70/134 (52.24%), Query Frame = -2 
Query:   91 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQP-SKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIED 486
            NG+++ + ++   ++R  LEL YA  L K+ N++ KA    +K  I   WT     M + ++VHK+LG  +Q E  K      ++  K KK ++ E+EK    + +  +++++ KKK     K  E  +  +++
Sbjct:   23 NGEIFCKTLMSALQQRSNLELSYAKGLEKVANKLTKALASVNKNCINNAWTCASDEMRTTAEVHKKLGTAIQQEAIKPTSQVLEEHEKKKKKLDNEVEKMTSSVLNNWKQQIKVKKKLMEHTKKHEALFHHVDN 156
[Close]
BLAST of SMED30016895 vs. Ensembl Mouse
Match: Nostrin (nitric oxide synthase trafficker [Source:MGI Symbol;Acc:MGI:3606242])

HSP 1 Score: 55.4546 bits (132), Expect = 4.348e-9
Identity = 37/138 (26.81%), Postives = 71/138 (51.45%), Query Frame = -2 
Query:   79 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQPSK-GSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNN 486
            +GD + +++  V ++R  LE+ YA  L K+  R+ KA Q +K   + T W     +M S +D+H++LG  ++ E  K          K +K ++ E+EK   ++ +   ++++ KKK   + K  E  +  +E  K +
Sbjct:   23 HGDNFCKQITSVLQQRANLEISYAKGLQKLAVRLSKALQSTKKNCLSTAWAWASESMKSAADLHQKLGKAIELEAIKPTHQVLSMQEKKRKSLDNEVEKTANLVINNWNQQIKAKKKLMMSTKKHEALFHLVESSKQS 160
[Close]
BLAST of SMED30016895 vs. UniProt/SwissProt
Match: sp|Q5I0D6|NOSTN_RAT (Nostrin OS=Rattus norvegicus OX=10116 GN=Nostrin PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.336e-8
Identity = 38/138 (27.54%), Postives = 71/138 (51.45%), Query Frame = -2 
Query:   79 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQPSK-GSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNN 486
            NGD + +++  V ++R  LE+ YA  L K+  ++ KA Q +K   + T W     +M S +D+H++LG  ++ E  K          K +K ++ E+EK   ++ +   ++++ KKK   + K  E  +  IE  K +
Sbjct:   23 NGDNFCKQITSVLQQRANLEISYAKGLQKLAVKLSKALQSTKKNCLSTAWAWASESMKSAADLHQKLGKAIELEAIKPTHQVLSMQEKKRKSLDNEVEKSANLVINNWNQQIKAKKKLMMSTKKHEALFHLIESSKQS 160
[Close]
BLAST of SMED30016895 vs. UniProt/SwissProt
Match: sp|Q8IVI9|NOSTN_HUMAN (Nostrin OS=Homo sapiens OX=9606 GN=NOSTRIN PE=1 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 2.328e-8
Identity = 40/154 (25.97%), Postives = 80/154 (51.95%), Query Frame = -2 
Query:   31 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGS-ICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSKVV 486
            NG+ + ++V  V ++R  LE+ YA  L K+ +++ KA Q ++ S + + W      M S +D+H++LG  ++ E  K      +   K +K ++ E+EK   ++     ++++ KKK   + K  E  ++ +E  K       S  +KE+ K++
Sbjct:   23 NGENFCKQVTSVLQQRANLEISYAKGLQKLASKLSKALQNTRKSCVSSAWAWASEGMKSTADLHQKLGKAIELEAIKPTYQVLNVQEKKRKSLDNEVEKTANLVISNWNQQIKAKKKLMVSTKKHEALFQLVESSKQ------SMTEKEKRKLL 170
[Close]
BLAST of SMED30016895 vs. UniProt/SwissProt
Match: sp|Q6WKZ7|NOSTN_MOUSE (Nostrin OS=Mus musculus OX=10090 GN=Nostrin PE=1 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 3.042e-8
Identity = 37/138 (26.81%), Postives = 71/138 (51.45%), Query Frame = -2 
Query:   79 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQPSK-GSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNN 486
            +GD + +++  V ++R  LE+ YA  L K+  R+ KA Q +K   + T W     +M S +D+H++LG  ++ E  K          K +K ++ E+EK   ++ +   ++++ KKK   + K  E  +  +E  K +
Sbjct:   23 HGDNFCKQITSVLQQRANLEISYAKGLQKLAVRLSKALQSTKKNCLSTAWAWASESMKSAADLHQKLGKAIELEAIKPTHQVLSMQEKKRKSLDNEVEKTANLVINNWNQQIKAKKKLMMSTKKHEALFHLVESSKQS 160
[Close]
BLAST of SMED30016895 vs. UniProt/SwissProt
Match: sp|Q2KJB5|NOSTN_BOVIN (Nostrin OS=Bos taurus OX=9913 GN=NOSTRIN PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.051e-7
Identity = 35/136 (25.74%), Postives = 65/136 (47.79%), Query Frame = -2 
Query:   85 NG-DLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSK-GSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYK 486
            NG D  +++  + ++R  LE+ YA  L K+  ++ K  Q +K   + + W      M S  D+H++LG  ++ E  K          K +K +E E+EK   ++     ++++ KKK   + K  E  +  +E  K
Sbjct:   23 NGEDFCKQITSILQQRANLEINYAKGLQKLATKLSKTLQSAKKNCLVSAWAWVSEGMKSAGDLHQKLGKAIELEAIKPAHQVLSAHEKKRKSLENEVEKTANLVISNWNQQIKAKKKLMVSTKKHEALFHLVESSK 158
[Close]
BLAST of SMED30016895 vs. TrEMBL
Match: A0A1S3IGK6 (nostrin-like isoform X8 OS=Lingula unguis OX=7574 GN=LOC106164098 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 6.546e-22
Identity = 54/151 (35.76%), Postives = 84/151 (55.63%), Query Frame = -2 
Query:   52 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGND 504
            RK +K   +  REV  + +ER ELE  YA  LSK+ N++ KA     G++ + W      M SE+++H+ L    Q++ YK L + S+   K +KP+E  ++K  K L ++REEE + +K AFS  K+ EK  E ++D K      +S  D
Sbjct:   61 RKHIKQGNEFSREVAAIVQERAELESSYAKSLSKLSNKLTKACACCIGTMVSAWGTAASEMESEAELHRGLATSFQEDIYKPLKVLSETQYKIRKPIENMVDKAAKGLLNRREEEQKMRKHAFSCCKETEKLSEQLQDAKAGKSKQMSDKD 211
[Close]
BLAST of SMED30016895 vs. TrEMBL
Match: A0A1S3IHK2 (nostrin-like isoform X7 OS=Lingula unguis OX=7574 GN=LOC106164098 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 8.054e-22
Identity = 54/151 (35.76%), Postives = 84/151 (55.63%), Query Frame = -2 
Query:   52 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGND 504
            RK +K   +  REV  + +ER ELE  YA  LSK+ N++ KA     G++ + W      M SE+++H+ L    Q++ YK L + S+   K +KP+E  ++K  K L ++REEE + +K AFS  K+ EK  E ++D K      +S  D
Sbjct:   61 RKHIKQGNEFSREVAAIVQERAELESSYAKSLSKLSNKLTKACACCIGTMVSAWGTAASEMESEAELHRGLATSFQEDIYKPLKVLSETQYKIRKPIENMVDKAAKGLLNRREEEQKMRKHAFSCCKETEKLSEQLQDAKAGKSKQMSDKD 211
[Close]
BLAST of SMED30016895 vs. TrEMBL
Match: A0A1S3IIL8 (nostrin-like isoform X5 OS=Lingula unguis OX=7574 GN=LOC106164098 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 8.315e-22
Identity = 54/151 (35.76%), Postives = 84/151 (55.63%), Query Frame = -2 
Query:   52 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGND 504
            RK +K   +  REV  + +ER ELE  YA  LSK+ N++ KA     G++ + W      M SE+++H+ L    Q++ YK L + S+   K +KP+E  ++K  K L ++REEE + +K AFS  K+ EK  E ++D K      +S  D
Sbjct:   61 RKHIKQGNEFSREVAAIVQERAELESSYAKSLSKLSNKLTKACACCIGTMVSAWGTAASEMESEAELHRGLATSFQEDIYKPLKVLSETQYKIRKPIENMVDKAAKGLLNRREEEQKMRKHAFSCCKETEKLSEQLQDAKAGKSKQMSDKD 211
[Close]
BLAST of SMED30016895 vs. TrEMBL
Match: A0A2R2MK69 (nostrin-like isoform X4 OS=Lingula unguis OX=7574 GN=LOC106164098 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 8.644e-22
Identity = 54/151 (35.76%), Postives = 84/151 (55.63%), Query Frame = -2 
Query:   52 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGND 504
            RK +K   +  REV  + +ER ELE  YA  LSK+ N++ KA     G++ + W      M SE+++H+ L    Q++ YK L + S+   K +KP+E  ++K  K L ++REEE + +K AFS  K+ EK  E ++D K      +S  D
Sbjct:   61 RKHIKQGNEFSREVAAIVQERAELESSYAKSLSKLSNKLTKACACCIGTMVSAWGTAASEMESEAELHRGLATSFQEDIYKPLKVLSETQYKIRKPIENMVDKAAKGLLNRREEEQKMRKHAFSCCKETEKLSEQLQDAKAGKSKQMSDKD 211
[Close]
BLAST of SMED30016895 vs. TrEMBL
Match: A0A1S3IGK1 (nostrin-like isoform X2 OS=Lingula unguis OX=7574 GN=LOC106164098 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 1.064e-21
Identity = 54/151 (35.76%), Postives = 84/151 (55.63%), Query Frame = -2 
Query:   52 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGND 504
            RK +K   +  REV  + +ER ELE  YA  LSK+ N++ KA     G++ + W      M SE+++H+ L    Q++ YK L + S+   K +KP+E  ++K  K L ++REEE + +K AFS  K+ EK  E ++D K      +S  D
Sbjct:   61 RKHIKQGNEFSREVAAIVQERAELESSYAKSLSKLSNKLTKACACCIGTMVSAWGTAASEMESEAELHRGLATSFQEDIYKPLKVLSETQYKIRKPIENMVDKAAKGLLNRREEEQKMRKHAFSCCKETEKLSEQLQDAKAGKSKQMSDKD 211
[Close]
BLAST of SMED30016895 vs. Ensembl Cavefish
Match: nostrin (nitric oxide synthase trafficking [Source:NCBI gene;Acc:103028777])

HSP 1 Score: 62.3882 bits (150), Expect = 1.565e-11
Identity = 41/136 (30.15%), Postives = 73/136 (53.68%), Query Frame = -2 
Query:   85 NGDLY-REVLQVTKERYELELEYATKLSKIGNRIIKAFQP-SKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYK 486
            NG+ + +E++ V ++R ELE+ YA  L K+  ++IK  +  S  S    W+   + M S +D H+ LG+ LQ +    +    D+  K KKP++  IEK  K++     E+++ KKK  +  ++ E  +  +E  K
Sbjct:   46 NGETFCKELVTVFQQRSELEINYAKGLQKLAGKLIKVSKAMSNNSTYNAWSLVSNEMFSIADAHRILGHALQQDTVLEIRQILDEHTKRKKPLDNTIEKSGKLVVTNWNEQLKVKKKLSALTREHEALFSFVEKNK 181
[Close]
BLAST of SMED30016895 vs. Ensembl Nematostella
Match: EDO43872 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXM7])

HSP 1 Score: 72.7886 bits (177), Expect = 1.099e-15
Identity = 47/152 (30.92%), Postives = 76/152 (50.00%), Query Frame = -2 
Query:   52 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSK-GSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGND 504
            RK  +   D  +E+  +  ER ++EL YA  LSK+  +  K       G++ + W     N+ SE+D+H+ L NQL  E  K +    +   K +KPVE E+EK  K   D+  + ++ K  + S  K+ E  ++ +ED K     +VS  D
Sbjct:   18 RKSCRQGSDFCKEIAAIFNERSKIELSYADSLSKLSVKAQKLLAKDVVGTLKSSWEKISWNIESEADIHRTLANQLHGEAAKQIKAFVETQSKTRKPVEVEVEKAYKNFSDRLSDSLKKKGASHSKSKEVETLHDQMEDTKQGKGKAVSDKD 169
[Close]
BLAST of SMED30016895 vs. Ensembl Medaka
Match: nostrin (nitric oxide synthase trafficking [Source:NCBI gene;Acc:101156693])

HSP 1 Score: 67.0106 bits (162), Expect = 3.357e-13
Identity = 43/141 (30.50%), Postives = 77/141 (54.61%), Query Frame = -2 
Query:   85 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQP-SKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYK 504
            +++ K   + ++E++ V ++R ELEL YA  L K+  R+IKA +  +K S  T W +    M S +D H+ LGN    E    +   SD+  K K+P+++ IE+  K+      ++++ KKK  +  ++ E  Y  +E+ K
Sbjct:   41 KQYSKDGENFFKELMAVFQQRAELELTYAKGLQKLAGRLIKASKGMTKNSTYTAWCHVSDEMYSRADAHRSLGNAFNQEAILEIRQVSDEHSKRKRPLDSVIERNGKLFHSNWSDQLKLKKKLIALTREHEALYNFVENNK 181
[Close]
BLAST of SMED30016895 vs. Planmine SMEST
Match: SMESG000032404.1 (SMESG000032404.1)

HSP 1 Score: 320.472 bits (820), Expect = 3.161e-113
Identity = 156/162 (96.30%), Postives = 158/162 (97.53%), Query Frame = -2 
Query:   19 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSKVVTYNL 504
            +KWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSK    +L
Sbjct:   20 KKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSKYTGRDL 181
[Close]
BLAST of SMED30016895 vs. Planmine SMEST
Match: SMESG000032404.1 (SMESG000032404.1)

HSP 1 Score: 322.398 bits (825), Expect = 5.592e-113
Identity = 155/157 (98.73%), Postives = 157/157 (100.00%), Query Frame = -2 
Query:   34 RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSKV 504
            +KWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSK+
Sbjct:   20 KKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSICTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETEIEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGNDKERSKI 176
The following BLAST results are available for this feature:
BLAST of SMED30016895 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
NOSTRIN4.848e-925.97nitric oxide synthase trafficking [Source:HGNC Sym... [more]
NOSTRIN5.788e-925.97nitric oxide synthase trafficking [Source:HGNC Sym... [more]
NOSTRIN5.788e-925.97nitric oxide synthase trafficking [Source:HGNC Sym... [more]
back to top
BLAST of SMED30016895 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30016895 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nost1.367e-2032.86gene:FBgn0259734 transcript:FBtr0343357[more]
Nost1.438e-2032.86gene:FBgn0259734 transcript:FBtr0302907[more]
Nost1.438e-2032.86gene:FBgn0259734 transcript:FBtr0300000[more]
Nost1.462e-2031.76gene:FBgn0259734 transcript:FBtr0302906[more]
Nost1.474e-2031.76gene:FBgn0259734 transcript:FBtr0300001[more]
back to top
BLAST of SMED30016895 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nostrin3.885e-1127.94nitric oxide synthase trafficking [Source:ZFIN;Acc... [more]
nostrin3.885e-1127.94nitric oxide synthase trafficking [Source:ZFIN;Acc... [more]
back to top
BLAST of SMED30016895 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
prag19.792e-927.61PEAK1 related, kinase-activating pseudokinase 1 [S... [more]
prag11.052e-827.61PEAK1 related, kinase-activating pseudokinase 1 [S... [more]
back to top
BLAST of SMED30016895 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Nostrin4.348e-926.81nitric oxide synthase trafficker [Source:MGI Symbo... [more]
back to top
BLAST of SMED30016895 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q5I0D6|NOSTN_RAT1.336e-827.54Nostrin OS=Rattus norvegicus OX=10116 GN=Nostrin P... [more]
sp|Q8IVI9|NOSTN_HUMAN2.328e-825.97Nostrin OS=Homo sapiens OX=9606 GN=NOSTRIN PE=1 SV... [more]
sp|Q6WKZ7|NOSTN_MOUSE3.042e-826.81Nostrin OS=Mus musculus OX=10090 GN=Nostrin PE=1 S... [more]
sp|Q2KJB5|NOSTN_BOVIN1.051e-725.74Nostrin OS=Bos taurus OX=9913 GN=NOSTRIN PE=1 SV=1[more]
back to top
BLAST of SMED30016895 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3IGK66.546e-2235.76nostrin-like isoform X8 OS=Lingula unguis OX=7574 ... [more]
A0A1S3IHK28.054e-2235.76nostrin-like isoform X7 OS=Lingula unguis OX=7574 ... [more]
A0A1S3IIL88.315e-2235.76nostrin-like isoform X5 OS=Lingula unguis OX=7574 ... [more]
A0A2R2MK698.644e-2235.76nostrin-like isoform X4 OS=Lingula unguis OX=7574 ... [more]
A0A1S3IGK11.064e-2135.76nostrin-like isoform X2 OS=Lingula unguis OX=7574 ... [more]
back to top
BLAST of SMED30016895 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nostrin1.565e-1130.15nitric oxide synthase trafficking [Source:NCBI gen... [more]
back to top
BLAST of SMED30016895 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30016895 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30016895 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO438721.099e-1530.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMED30016895 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nostrin3.357e-1330.50nitric oxide synthase trafficking [Source:NCBI gen... [more]
back to top
BLAST of SMED30016895 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000032404.13.161e-11396.30SMESG000032404.1[more]
SMESG000032404.15.592e-11398.73SMESG000032404.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016895 ID=SMED30016895|Name=SMED30016895|organism=Schmidtea mediterranea sexual|type=transcript|length=505bp
AGGTAAATTAACTGAAAATAAATTATAAGTAACTACCTTCGACCGTTCTT
TATCATTCCCGGAAACTGAAGATGGATGATTATTCTTATAATCTTCAATA
CTTTCATATGACTTTTCACAATCCTTTACTGCCGAGAATGCTTTCTTTTT
ACATCTCATTTCTTCCTCCCTTTTATCTTGTAATATTTTCAAACATTTTT
CAATTTCGGTTTCTACAGGTTTTTTGAATTTAATAAATTGATCACTTGCG
ATAACAAGAGATTTGTAAAATTCGTCTTGCAATTGATTACCCAGACGCTT
ATGAACATCAGATTCACTAATCATATTATGTCCAGTATTTGTCCAAAGAG
TACAAATAGACCCTTTGCTAGGTTGGAATGCCTTTATAATACGATTGCCA
ATTTTTGACAATTTGGTTGCATATTCTAATTCTAACTCATATCTTTCTTT
TGTAACTTGCAAAACTTCCCTGTATAAATCTCCATTAATTTTAAGCCATT
TTCTT
back to top

protein sequence of SMED30016895-orf-1

>SMED30016895-orf-1 ID=SMED30016895-orf-1|Name=SMED30016895-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=168bp
RKWLKINGDLYREVLQVTKERYELELEYATKLSKIGNRIIKAFQPSKGSI
CTLWTNTGHNMISESDVHKRLGNQLQDEFYKSLVIASDQFIKFKKPVETE
IEKCLKILQDKREEEMRCKKKAFSAVKDCEKSYESIEDYKNNHPSSVSGN
DKERSKVVTYNLFSVNLP
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR027267AH/BAR_dom_sf
IPR028535Nostrin
IPR001060FCH_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027267AH/BAR domain superfamilyGENE3DG3DSA:1.20.1270.60coord: 1..158
e-value: 1.3E-19
score: 72.6
IPR027267AH/BAR domain superfamilySUPERFAMILYSSF103657BAR/IMD domain-likecoord: 1..155
IPR001060FCH domainPFAMPF00611FCHcoord: 2..68
e-value: 4.2E-7
score: 30.1
IPR028535NostrinPANTHERPTHR14167:SF31NOSTRINcoord: 6..141
NoneNo IPR availablePANTHERPTHR14167SH3 DOMAIN-CONTAININGcoord: 6..141