Serine/threonine-protein kinase Nek4
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016820 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Human
Match: NEK4 (NIMA related kinase 4 [Source:HGNC Symbol;Acc:HGNC:11399]) HSP 1 Score: 339.732 bits (870), Expect = 1.063e-104 Identity = 159/274 (58.03%), Postives = 206/274 (75.18%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSL-YIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
Y +++ +G+GSYGEV LVKH+ D K++V+KK++ +S RE AA E QLL L+HPNIV YK+S+E + L YI M FCE GDLY +LKE+KG LL E Q++EW +QI MAL Y+HE++ILHRDLKTQN+FLT++ IIK+GDLGIA+ LE+ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MP Y+ EL LI+ ML+K PE+RPS IL +I+ I F+E +K K K
Sbjct: 6 YCYLRVVGKGSYGEVTLVKHR-RDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSK 278
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Human
Match: NEK4 (NIMA related kinase 4 [Source:HGNC Symbol;Acc:HGNC:11399]) HSP 1 Score: 339.732 bits (870), Expect = 3.356e-104 Identity = 159/274 (58.03%), Postives = 206/274 (75.18%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSL-YIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
Y +++ +G+GSYGEV LVKH+ D K++V+KK++ +S RE AA E QLL L+HPNIV YK+S+E + L YI M FCE GDLY +LKE+KG LL E Q++EW +QI MAL Y+HE++ILHRDLKTQN+FLT++ IIK+GDLGIA+ LE+ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MP Y+ EL LI+ ML+K PE+RPS IL +I+ I F+E +K K K
Sbjct: 6 YCYLRVVGKGSYGEVTLVKHR-RDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSK 278
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Human
Match: NEK1 (NIMA related kinase 1 [Source:HGNC Symbol;Acc:HGNC:7744]) HSP 1 Score: 280.411 bits (716), Expect = 3.412e-80 Identity = 129/264 (48.86%), Postives = 185/264 (70.08%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y ++KIG+GS+G+ LVK + D +++V+K+I+ +R S +E E +R E +L +++HPNIVQY++SFEEN SLYI M +CE GDL+ R+ +KG L E QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK +++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YE+ TLKHAF A + L KI+ G P + Y+ +L +L+ + ++P RPS IL FI I F+
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTE-DGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Human
Match: NEK1 (NIMA related kinase 1 [Source:HGNC Symbol;Acc:HGNC:7744]) HSP 1 Score: 280.411 bits (716), Expect = 4.555e-80 Identity = 129/264 (48.86%), Postives = 185/264 (70.08%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y ++KIG+GS+G+ LVK + D +++V+K+I+ +R S +E E +R E +L +++HPNIVQY++SFEEN SLYI M +CE GDL+ R+ +KG L E QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK +++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YE+ TLKHAF A + L KI+ G P + Y+ +L +L+ + ++P RPS IL FI I F+
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTE-DGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Human
Match: NEK1 (NIMA related kinase 1 [Source:HGNC Symbol;Acc:HGNC:7744]) HSP 1 Score: 280.026 bits (715), Expect = 4.733e-80 Identity = 129/264 (48.86%), Postives = 185/264 (70.08%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y ++KIG+GS+G+ LVK + D +++V+K+I+ +R S +E E +R E +L +++HPNIVQY++SFEEN SLYI M +CE GDL+ R+ +KG L E QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK +++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YE+ TLKHAF A + L KI+ G P + Y+ +L +L+ + ++P RPS IL FI I F+
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTE-DGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Celegans
Match: nekl-2 (Serine/threonine-protein kinase nekl-2 [Source:UniProtKB/Swiss-Prot;Acc:O01775]) HSP 1 Score: 190.66 bits (483), Expect = 4.968e-54 Identity = 102/293 (34.81%), Postives = 170/293 (58.02%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHK-DYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKG-------SLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLT--KSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKPCRHRKTSLS 965
NY ++ +G+G++G WL + K D H+K ++K I+ +++E + + E LLK ++HP I+ Y DSF +N L I M + E G L + +++ EK ++++ QI +AL+++H++NI+HRDLK QNI + K+++K+ D GI+K+L +K A TVIGTP Y+SPE+ E +PYN KSD+WSLGCV YE+ L+ AF+ +N A+ KI + + + ++ L++ +L +KRP ++L + + ++ R P RK S S
Sbjct: 3 NYEKVRVVGRGAFGVCWLCRGKNDASHQKVIIKLINTHGMTEKEENSIQSEVNLLKKVQHPLIIGYIDSFIMDNQLGIVMQYAEGGTLERLINDQRAIKDSNMREYFPEKTVLDYFTQILIALNHMHQKNIVHRDLKPQNILMNRRKTVLKLSDFGISKELGTK-SAASTVIGTPNYLSPEICESRPYNQKSDMWSLGCVLYELLQLERAFDGENLPAIVMKITRSKQNPLGDHVSNDVKMLVENLLKTHTDKRPDVSQLLSDPLVLPYLISIHCDLGRIEPPPTDKRKPSAS 294
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Celegans
Match: nekl-1 (NEK (NEver in mitosis Kinase) Like [Source:UniProtKB/TrEMBL;Acc:A0A061AD48]) HSP 1 Score: 195.667 bits (496), Expect = 4.601e-52 Identity = 103/253 (40.71%), Postives = 153/253 (60.47%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQL--ESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKIL 869
Y I+ +G+G++G L + ++ D ++K+I+ + A E LL +EHPNI+ Y DSFEE L I M + + G L ++ R +LL E+QI + +IQ+ A++Y+HE ++LHRDLKT N+FLT+ S +KIGD GI+K + E+ AKTV+GTPYY+SPE+ YN KSD+W+LGC+ YEM LK AF N AL I+ + Y+ E+ +I+ +L P+KRPS + L
Sbjct: 171 YERIRTVGKGAFGSAVLYRRRE-DSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTL-AQMLSRTQNLLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQAL 421
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Celegans
Match: nekl-1 (NEK (NEver in mitosis Kinase) Like [Source:UniProtKB/TrEMBL;Acc:A0A061AD48]) HSP 1 Score: 195.667 bits (496), Expect = 4.815e-52 Identity = 103/253 (40.71%), Postives = 153/253 (60.47%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQL--ESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKIL 869
Y I+ +G+G++G L + ++ D ++K+I+ + A E LL +EHPNI+ Y DSFEE L I M + + G L ++ R +LL E+QI + +IQ+ A++Y+HE ++LHRDLKT N+FLT+ S +KIGD GI+K + E+ AKTV+GTPYY+SPE+ YN KSD+W+LGC+ YEM LK AF N AL I+ + Y+ E+ +I+ +L P+KRPS + L
Sbjct: 133 YERIRTVGKGAFGSAVLYRRRE-DSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTL-AQMLSRTQNLLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQAL 383
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Celegans
Match: nekl-3 (Serine/threonine-protein kinase nekl-3 [Source:UniProtKB/Swiss-Prot;Acc:G5EFM9]) HSP 1 Score: 172.94 bits (437), Expect = 2.480e-48 Identity = 99/256 (38.67%), Postives = 151/256 (58.98%), Query Frame = 3
Query: 132 KKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLEC----QLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERK--GSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLT-KSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAF--NAKNYTALSYKILMGQTPDMPSK-YNKELINLIQAMLNKSPEKRPSTGKIL 869
KKIG+G + EV+ + D LKKI D++ AR +C LLK L H N+++Y SF +NN L I + E+GD+ +K K G L+ EK I ++ +Q+ AL+++H + I+HRD+K N+F+T I+K+GDLG+ + SK A +++GTPYYMSPE ++ YNFKSD+WS GC+ YEMA L+ F + N +L KI + P +P+ Y+ +L +L+ + KRP T ++L
Sbjct: 27 KKIGKGQFSEVFRAQCTWVD-LHVALKKIQVFEMVDQK---ARQDCLKEIDLLKQLNHVNVIRYYASFIDNNQLNIVLELAEAGDMSRMIKHFKKGGRLIPEKTIWKYFVQLARALAHMHSKRIMHRDIKPANVFITGNGIVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIQESGYNFKSDLWSTGCLLYEMAALQSPFYGDKMNLYSLCKKIENCEYPPLPADIYSTQLRDLVSRCILPEASKRPETSEVL 278
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Celegans
Match: nekl-3 (Serine/threonine-protein kinase nekl-3 [Source:UniProtKB/Swiss-Prot;Acc:G5EFM9]) HSP 1 Score: 172.94 bits (437), Expect = 2.480e-48 Identity = 99/256 (38.67%), Postives = 151/256 (58.98%), Query Frame = 3
Query: 132 KKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLEC----QLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERK--GSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLT-KSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAF--NAKNYTALSYKILMGQTPDMPSK-YNKELINLIQAMLNKSPEKRPSTGKIL 869
KKIG+G + EV+ + D LKKI D++ AR +C LLK L H N+++Y SF +NN L I + E+GD+ +K K G L+ EK I ++ +Q+ AL+++H + I+HRD+K N+F+T I+K+GDLG+ + SK A +++GTPYYMSPE ++ YNFKSD+WS GC+ YEMA L+ F + N +L KI + P +P+ Y+ +L +L+ + KRP T ++L
Sbjct: 27 KKIGKGQFSEVFRAQCTWVD-LHVALKKIQVFEMVDQK---ARQDCLKEIDLLKQLNHVNVIRYYASFIDNNQLNIVLELAEAGDMSRMIKHFKKGGRLIPEKTIWKYFVQLARALAHMHSKRIMHRDIKPANVFITGNGIVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIQESGYNFKSDLWSTGCLLYEMAALQSPFYGDKMNLYSLCKKIENCEYPPLPADIYSTQLRDLVSRCILPEASKRPETSEVL 278
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Fly
Match: niki (gene:FBgn0045980 transcript:FBtr0084737) HSP 1 Score: 197.593 bits (501), Expect = 6.387e-53 Identity = 107/257 (41.63%), Postives = 152/257 (59.14%), Query Frame = 3
Query: 105 PFTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSL-LCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKIL 869
P NY ++ +GQGS+G L + K D + V K+I+ + S + A E + L HPNIV Y SF ++N+L I M + + G L + ER+G L E+ II QI+ A++Y+H NILHRDLKT N+FL + I+KIGD GI+K + +K A+TV+GTPYY SPE+ E K Y+ KSDIW+LGC+ EM LK F A N + L KI+ G +PS Y L +L+ +L +RP+ ++L
Sbjct: 100 PELANYEKVRVVGQGSFGIAILYRRKS-DGHQIVFKQINLSELSPPGRDLAMNEVDVFSKLHHPNIVSYLGSFIKDNTLLIEMEYADGGTLAQIIAERQGKLHFPERYIIAVFEQISSAINYMHSENILHRDLKTANVFLNRRGIVKIGDFGISKIMNTKIH-AQTVLGTPYYFSPEMCEGKEYDNKSDIWALGCILGEMCCLKKTFAASNLSELVTKIMAGNYTPVPSGYTSGLRSLMSNLLQVEAPRRPTASEVL 354
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Fly
Match: Nek2 (gene:FBgn0029970 transcript:FBtr0071142) HSP 1 Score: 157.532 bits (397), Expect = 9.622e-40 Identity = 90/270 (33.33%), Postives = 142/270 (52.59%), Query Frame = 3
Query: 114 KNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSF--EENNSLYIAMAFCESGDLYTRLKERKGSL--LCEKQIIEWLIQITMALSYIHER----NILHRDLKTQNIFLTKSI-IKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHI 896
++Y + +G GS+G + V+ K + F K +++ + + +A E +L+ L+HPNIVQY E S+YI M C GDL ++ + E I L Q+ AL H + ILHRD+K NIFL + K+GD G+A+ L A + +GTP+YMSPEL + + Y+ KSD+W++GC+ YEM L+ F + + LS KI G+ +P+ Y+ +L +I ML E+RP I+ + + +I
Sbjct: 17 QDYEVLAVMGNGSFGTCYKVRDKSTG-ELFAWKGMNYDELDEAKCDALVSEISVLRQLQHPNIVQYYHHLVNREAKSVYIVMECCAGGDLAQIVQRARSQRQRFEEPYIWRVLFQLCRALQVCHNKIPNGTILHRDIKPANIFLDAAGNAKLGDFGLARMLRRDQSFAASFVGTPHYMSPELVKGRKYDRKSDVWAVGCLVYEMCALRPPFRGRAFDQLSEKIAQGEFSRIPAIYSTDLQEIIAFMLAVDHEQRPGIEVIIRHPLVVRNI 285
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Fly
Match: polo (gene:FBgn0003124 transcript:FBtr0074839) HSP 1 Score: 144.821 bits (364), Expect = 4.331e-36 Identity = 88/273 (32.23%), Postives = 148/273 (54.21%), Query Frame = 3
Query: 114 KNYSFIKKIGQGSYG---EVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSI-IKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIR-DHIHMFVEQS 917
K Y ++ G+G + E+ V+ D K V KK+ + + E E + +SL HPNIV++ + FE++ ++YI + C+ + K RK + E + ++ QI + Y+H+ I+HRDLK N+FL + +KIGD G+A ++E + + KT+ GTP Y++PE+ KK ++F+ DIWS+GCV Y + + F K KI + +PS K +++ AML +PE RP+ G++L F++ + MF+ S
Sbjct: 23 KTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHN---QKEKTAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKS--ITEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEY-RVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKVPMFLPSS 289
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Fly
Match: polo (gene:FBgn0003124 transcript:FBtr0100318) HSP 1 Score: 144.821 bits (364), Expect = 4.331e-36 Identity = 88/273 (32.23%), Postives = 148/273 (54.21%), Query Frame = 3
Query: 114 KNYSFIKKIGQGSYG---EVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSI-IKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIR-DHIHMFVEQS 917
K Y ++ G+G + E+ V+ D K V KK+ + + E E + +SL HPNIV++ + FE++ ++YI + C+ + K RK + E + ++ QI + Y+H+ I+HRDLK N+FL + +KIGD G+A ++E + + KT+ GTP Y++PE+ KK ++F+ DIWS+GCV Y + + F K KI + +PS K +++ AML +PE RP+ G++L F++ + MF+ S
Sbjct: 23 KTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHN---QKEKTAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKS--ITEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEY-RVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKVPMFLPSS 289
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Fly
Match: fu (gene:FBgn0001079 transcript:FBtr0074602) HSP 1 Score: 146.362 bits (368), Expect = 5.336e-36 Identity = 86/274 (31.39%), Postives = 142/274 (51.82%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSI-IKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKP 938
Y+ +GQGS+G V+ KD D K +K IS + +E++ R EC + L+HP++++ +SFE L++ F DL+ L + E+ + AL Y+H ILHRDLK QN+ L K++ K+ D G+A+ + + ++ GTP YM+PEL ++PY+ +D+WSLGC+ YE + F A + L K++ + PS E + +Q +L K P R S ++LC+ F+ I + Q++ + P
Sbjct: 4 YAVSSMVGQGSFGCVYKATRKD-DSKVVAIKVISKRGRATKELKNLRRECDIQARLKHPHVIEMIESFESKTDLFVVTEFALM-DLHRYLSYNGA--MGEEPARRVTGHLVSALYYLHSNRILHRDLKPQNVLLDKNMHAKLCDFGLARNMTLGTHVLTSIKGTPLYMAPELLAEQPYDHHADMWSLGCIAYESMAGQPPFCASSILHL-VKMIKHEDVKWPSTLTSECRSFLQGLLEKDPGLRISWTQLLCHPFVEGRIFIAETQAEAAKESP 272
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Zebrafish
Match: nek4 (NIMA-related kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-1392]) HSP 1 Score: 347.436 bits (890), Expect = 2.266e-107 Identity = 164/275 (59.64%), Postives = 209/275 (76.00%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFE-ENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
Y F++ +G+GSYGEV LV+HK D K++V+KK++ SS RE AA E QLL L+HPNIV Y++S+E E+ LYI M FCE GDLY RLK++KG LL E+Q++EW +QI MAL Y+HE++ILHRDLKTQNIFLTK+ IIK+GDLGIA+ LE++ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MPSKY+ +L LI+ ML K PE RP IL +I+ I MF+E +K K K
Sbjct: 3 GYLFVRVVGKGSYGEVNLVRHKS-DRKQYVIKKLNLRTSSRRERRAAEQEAQLLSQLKHPNIVMYRESWEGEDCQLYIVMGFCEGGDLYHRLKQQKGELLPERQVVEWFVQIAMALQYLHEKHILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIVEGKLPQMPSKYDPQLGELIKRMLCKKPEDRPDVKHILRQPYIKHQISMFLEATKEKTAK 276
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Zebrafish
Match: nek4 (NIMA-related kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-1392]) HSP 1 Score: 347.821 bits (891), Expect = 2.297e-107 Identity = 164/275 (59.64%), Postives = 209/275 (76.00%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFE-ENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
Y F++ +G+GSYGEV LV+HK D K++V+KK++ SS RE AA E QLL L+HPNIV Y++S+E E+ LYI M FCE GDLY RLK++KG LL E+Q++EW +QI MAL Y+HE++ILHRDLKTQNIFLTK+ IIK+GDLGIA+ LE++ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MPSKY+ +L LI+ ML K PE RP IL +I+ I MF+E +K K K
Sbjct: 3 GYLFVRVVGKGSYGEVNLVRHKS-DRKQYVIKKLNLRTSSRRERRAAEQEAQLLSQLKHPNIVMYRESWEGEDCQLYIVMGFCEGGDLYHRLKQQKGELLPERQVVEWFVQIAMALQYLHEKHILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIVEGKLPQMPSKYDPQLGELIKRMLCKKPEDRPDVKHILRQPYIKHQISMFLEATKEKTAK 276
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Zebrafish
Match: nek1 (NIMA-related kinase 1 [Source:ZFIN;Acc:ZDB-GENE-040730-1]) HSP 1 Score: 278.1 bits (710), Expect = 1.190e-87 Identity = 129/264 (48.86%), Postives = 187/264 (70.83%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y +KKIG+GS+G+ LVK + D +++V+K+I +R S++E + +R E +L ++ HPNIVQYK+SFEE+ LYI M +CE GDL+ ++ ++GSL E+QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK +++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YEM TLKHAF A N L KI+ G P + Y+ +L +L+ + ++P +RPS IL F+ IH F+
Sbjct: 4 YERLKKIGEGSFGKAILVKSRT-DGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Zebrafish
Match: CABZ01040556.1 (NIMA related kinase 3 [Source:NCBI gene;Acc:100536894]) HSP 1 Score: 232.646 bits (592), Expect = 1.862e-68 Identity = 110/261 (42.15%), Postives = 171/261 (65.52%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIH 899
YS ++ IG+GS+G LV+ ++ K +V+K+I ++ + M+ +R E LL ++HPNIV ++DSFE + LYI M FC GDL R++++ L E+QI++W Q+ + +IH++ +LHRDLK++NIFLT S +K+GD G A L S A+T +GTPYY+SPE+++ KPYN KSD+WSLGCV YE+ TL+H F A+++ +L ++ G +PS + EL LI+ M +P+ RPS IL ++ + +H
Sbjct: 4 YSVVRVIGEGSFGRALLVQ-PIHEEKHYVMKEIRLPKA-ESGMKKSRSEAVLLSKMKHPNIVAFRDSFEADGHLYIVMEFCSGGDLMQRIRQQNRLLFSEEQILKWFAQMCLGTKHIHDKRVLHRDLKSKNIFLTDSGTVKVGDFGSACSLNSAKAYAQTYVGTPYYVSPEIWDNKPYNNKSDVWSLGCVLYELCTLQHPFQARSWKSLILQVCRGSYAPLPSHFPYELHYLIKHMFKTNPKDRPSIHTILSSHRVSRLLH 262
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Zebrafish
Match: nek11 (NIMA-related kinase 11 [Source:ZFIN;Acc:ZDB-GENE-100922-73]) HSP 1 Score: 236.498 bits (602), Expect = 3.977e-68 Identity = 137/346 (39.60%), Postives = 190/346 (54.91%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDY--DHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERK--GSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHI----HMFVEQSKRKFDKPCRHRKTSLSKNLPGEFHKELYNYQDKSLGNKVS----------EVSDENNDKLKTTVDTSTLKNH 1103
Y +++G+GS+G V+LVK + + VLK+I E A E QLL L HP I+++ SF E ++ I FCE DL +L+E K G L E Q+ EWL Q+ + + YIH+R ILHRDLK +NIFL K+I+KIGD G++ L D+A T GTPYYMSPE K Y+ KSDIWSLGC+ YEM L HAF A+N+ A+ +I+ G TP +P Y+++L +L+Q+ML K R S L N FI + + H F S R + + + L + H L QD+S K+S + +DE KLK V+ +NH
Sbjct: 31 YIIQQRLGKGSFGTVYLVKDTKAVAEERLKVLKEIPVGDLKPNETVHATQEAQLLSQLSHPAILKFYSSFLERDAFCIITEFCEDKDLDCKLEELKHTGKTLSEPQVCEWLCQLLLGVHYIHQRRILHRDLKAKNIFLRKNIVKIGDFGVSCFLMGSCDLATTFTGTPYYMSPEALSHKGYDSKSDIWSLGCILYEMCCLAHAFEAQNFLAVVLRIVEGPTPSLPETYSRQLNSLMQSMLEKDSSHRISAADALRNRFIEESLQSMKHKFSILSLRDKSIGGERDASQILRALQKKVH--LQTLQDRSEVQKMSPRERMRLRKLQAADEKARKLKQIVEEKYQENH 374
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Xenopus
Match: nek1 (NIMA-related kinase 1 [Source:NCBI gene;Acc:100036653]) HSP 1 Score: 283.108 bits (723), Expect = 5.766e-81 Identity = 131/264 (49.62%), Postives = 184/264 (69.70%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y + KIG+GS+G+ LVK KD D K++V+K+I ++ S++E E +R E +L +++HPNIVQY++SFEE+ LYI M +CE GDL+ R+ +KG L E QI++W +Q+ +AL ++H+R ILHRD+K+QNIFLTK I++GD GIA+ L S ++A+T IGTPYY+SPE+ E +PYN KSDIW+LGCV YEM TLKHAF A N L KI+ G P + Y+ +L NL+ + ++P RPS IL FI I F+
Sbjct: 4 YVRVSKIGEGSFGKAILVKSKD-DGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Xenopus
Match: nek1 (NIMA-related kinase 1 [Source:NCBI gene;Acc:100036653]) HSP 1 Score: 283.108 bits (723), Expect = 5.911e-81 Identity = 131/264 (49.62%), Postives = 184/264 (69.70%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y + KIG+GS+G+ LVK KD D K++V+K+I ++ S++E E +R E +L +++HPNIVQY++SFEE+ LYI M +CE GDL+ R+ +KG L E QI++W +Q+ +AL ++H+R ILHRD+K+QNIFLTK I++GD GIA+ L S ++A+T IGTPYY+SPE+ E +PYN KSDIW+LGCV YEM TLKHAF A N L KI+ G P + Y+ +L NL+ + ++P RPS IL FI I F+
Sbjct: 4 YVRVSKIGEGSFGKAILVKSKD-DGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Xenopus
Match: nek1 (NIMA-related kinase 1 [Source:NCBI gene;Acc:100036653]) HSP 1 Score: 283.108 bits (723), Expect = 6.290e-81 Identity = 131/264 (49.62%), Postives = 184/264 (69.70%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y + KIG+GS+G+ LVK KD D K++V+K+I ++ S++E E +R E +L +++HPNIVQY++SFEE+ LYI M +CE GDL+ R+ +KG L E QI++W +Q+ +AL ++H+R ILHRD+K+QNIFLTK I++GD GIA+ L S ++A+T IGTPYY+SPE+ E +PYN KSDIW+LGCV YEM TLKHAF A N L KI+ G P + Y+ +L NL+ + ++P RPS IL FI I F+
Sbjct: 4 YVRVSKIGEGSFGKAILVKSKD-DGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Xenopus
Match: nek1 (NIMA-related kinase 1 [Source:NCBI gene;Acc:100036653]) HSP 1 Score: 283.108 bits (723), Expect = 7.163e-81 Identity = 131/264 (49.62%), Postives = 184/264 (69.70%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y + KIG+GS+G+ LVK KD D K++V+K+I ++ S++E E +R E +L +++HPNIVQY++SFEE+ LYI M +CE GDL+ R+ +KG L E QI++W +Q+ +AL ++H+R ILHRD+K+QNIFLTK I++GD GIA+ L S ++A+T IGTPYY+SPE+ E +PYN KSDIW+LGCV YEM TLKHAF A N L KI+ G P + Y+ +L NL+ + ++P RPS IL FI I F+
Sbjct: 4 YVRVSKIGEGSFGKAILVKSKD-DGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Xenopus
Match: sez6l2 (seizure related 6 homolog like 2 [Source:Xenbase;Acc:XB-GENE-921456]) HSP 1 Score: 243.432 bits (620), Expect = 5.695e-74 Identity = 124/300 (41.33%), Postives = 183/300 (61.00%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK--SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHI--HMFVEQSKRKFDKPCRHRKTSLS---------KNLPG 980
Y ++ IG+G++G+ +L K K D+ + V+K+I+ ++ +E EA+ E LL ++HPNIV + S EE N LYI M +C+ GDL R+ +++G L E QI+ W +QI++ L +IH+R +LHRD+K QNIFL+ ++ K+GD GIA+ L + ++A+T +GTPYY+SPE+ E KPYN K+DIWSLGCV YE+ LKH F A + L KI G+ +P+KY+ +L L+ + S RPS IL F+ I H+ E + +F HRK S K LPG
Sbjct: 4 YDIVRMIGEGAFGKAYLAKGKS-DNMQCVIKEINLSKMPTKEKEASHKEVVLLAKMKHPNIVTFFSSIEERNKLYIVMEYCDGGDLMKRVNKQRGVLFEEDQILSWFVQISLGLKHIHDRKVLHRDIKAQNIFLSNNGTLAKLGDFGIARMLNNTMELARTCVGTPYYLSPEICENKPYNNKTDIWSLGCVLYELCALKHPFEASSLRQLVLKICRGRYEPIPTKYSYDLRILVSQLFKISSRDRPSINSILKKPFLEKRINKHLSPELIEEEFSHTVIHRKKPSSNPARYPCKPKQLPG 302
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Mouse
Match: Nek4 (NIMA (never in mitosis gene a)-related expressed kinase 4 [Source:MGI Symbol;Acc:MGI:1344404]) HSP 1 Score: 337.035 bits (863), Expect = 2.504e-110 Identity = 157/269 (58.36%), Postives = 206/269 (76.58%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSL-YIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSK 920
Y +++ +G+GSYGEV LVKH+ D K++V+KK++ +S RE AA E QLL L+HPNIV YK+S+E + L YI M FCE GDLY +LKE+KG LL E Q++EW +QI MAL Y+HE++ILHRDLKTQN+FLT++ IIK+GDLGIA+ LE+ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MP Y+ EL LI+ ML++ PE+RPS IL +I+ HI +F+E +K
Sbjct: 6 YCYMRVVGRGSYGEVTLVKHRR-DGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATK 273
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Mouse
Match: Nek4 (NIMA (never in mitosis gene a)-related expressed kinase 4 [Source:MGI Symbol;Acc:MGI:1344404]) HSP 1 Score: 343.199 bits (879), Expect = 1.354e-106 Identity = 159/274 (58.03%), Postives = 208/274 (75.91%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSL-YIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
Y +++ +G+GSYGEV LVKH+ D K++V+KK++ +S RE AA E QLL L+HPNIV YK+S+E + L YI M FCE GDLY +LKE+KG LL E Q++EW +QI MAL Y+HE++ILHRDLKTQN+FLT++ IIK+GDLGIA+ LE+ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MP Y+ EL LI+ ML++ PE+RPS IL +I+ HI +F+E +K K K
Sbjct: 6 YCYMRVVGRGSYGEVTLVKHR-RDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSK 278
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Mouse
Match: Nek4 (NIMA (never in mitosis gene a)-related expressed kinase 4 [Source:MGI Symbol;Acc:MGI:1344404]) HSP 1 Score: 344.354 bits (882), Expect = 1.531e-106 Identity = 159/274 (58.03%), Postives = 208/274 (75.91%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSL-YIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
Y +++ +G+GSYGEV LVKH+ D K++V+KK++ +S RE AA E QLL L+HPNIV YK+S+E + L YI M FCE GDLY +LKE+KG LL E Q++EW +QI MAL Y+HE++ILHRDLKTQN+FLT++ IIK+GDLGIA+ LE+ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MP Y+ EL LI+ ML++ PE+RPS IL +I+ HI +F+E +K K K
Sbjct: 6 YCYMRVVGRGSYGEVTLVKHR-RDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSK 278
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Mouse
Match: Nek4 (NIMA (never in mitosis gene a)-related expressed kinase 4 [Source:MGI Symbol;Acc:MGI:1344404]) HSP 1 Score: 314.309 bits (804), Expect = 8.395e-96 Identity = 145/247 (58.70%), Postives = 187/247 (75.71%), Query Frame = 3
Query: 201 FVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSL-YIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
+V+KK++ +S RE AA E QLL L+HPNIV YK+S+E + L YI M FCE GDLY +LKE+KG LL E Q++EW +QI MAL Y+HE++ILHRDLKTQN+FLT++ IIK+GDLGIA+ LE+ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MP Y+ EL LI+ ML++ PE+RPS IL +I+ HI +F+E +K K K
Sbjct: 25 YVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSK 271
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Mouse
Match: Nek1 (NIMA (never in mitosis gene a)-related expressed kinase 1 [Source:MGI Symbol;Acc:MGI:97303]) HSP 1 Score: 284.648 bits (727), Expect = 6.391e-82 Identity = 131/264 (49.62%), Postives = 186/264 (70.45%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y ++KIG+GS+G+ LVK + D + +V+K+I+ +R SD+E + +R E +L +++HPNIVQYK+SFEEN SLYI M +CE GDL+ R+ +KG+L E QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK +++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YE+ TLKHAF A N L KI+ G P + Y+ +L +L+ + ++P RPS IL FI I F+
Sbjct: 4 YVRLQKIGEGSFGKAVLVKSTE-DGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. UniProt/SwissProt
Match: sp|Q9Z1J2|NEK4_MOUSE (Serine/threonine-protein kinase Nek4 OS=Mus musculus OX=10090 GN=Nek4 PE=1 SV=1) HSP 1 Score: 344.354 bits (882), Expect = 1.152e-105 Identity = 159/274 (58.03%), Postives = 208/274 (75.91%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSL-YIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
Y +++ +G+GSYGEV LVKH+ D K++V+KK++ +S RE AA E QLL L+HPNIV YK+S+E + L YI M FCE GDLY +LKE+KG LL E Q++EW +QI MAL Y+HE++ILHRDLKTQN+FLT++ IIK+GDLGIA+ LE+ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MP Y+ EL LI+ ML++ PE+RPS IL +I+ HI +F+E +K K K
Sbjct: 6 YCYMRVVGRGSYGEVTLVKHR-RDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSK 278
BLAST of Serine/threonine-protein kinase Nek4 vs. UniProt/SwissProt
Match: sp|P51957|NEK4_HUMAN (Serine/threonine-protein kinase Nek4 OS=Homo sapiens OX=9606 GN=NEK4 PE=1 SV=2) HSP 1 Score: 339.732 bits (870), Expect = 1.612e-103 Identity = 159/274 (58.03%), Postives = 206/274 (75.18%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSL-YIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
Y +++ +G+GSYGEV LVKH+ D K++V+KK++ +S RE AA E QLL L+HPNIV YK+S+E + L YI M FCE GDLY +LKE+KG LL E Q++EW +QI MAL Y+HE++ILHRDLKTQN+FLT++ IIK+GDLGIA+ LE+ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEMATLKHAFNAK+ +L Y+I+ G+ P MP Y+ EL LI+ ML+K PE+RPS IL +I+ I F+E +K K K
Sbjct: 6 YCYLRVVGKGSYGEVTLVKHR-RDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSK 278
BLAST of Serine/threonine-protein kinase Nek4 vs. UniProt/SwissProt
Match: sp|P51954|NEK1_MOUSE (Serine/threonine-protein kinase Nek1 OS=Mus musculus OX=10090 GN=Nek1 PE=1 SV=2) HSP 1 Score: 284.648 bits (727), Expect = 4.899e-81 Identity = 131/264 (49.62%), Postives = 186/264 (70.45%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y ++KIG+GS+G+ LVK + D + +V+K+I+ +R SD+E + +R E +L +++HPNIVQYK+SFEEN SLYI M +CE GDL+ R+ +KG+L E QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK +++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YE+ TLKHAF A N L KI+ G P + Y+ +L +L+ + ++P RPS IL FI I F+
Sbjct: 4 YVRLQKIGEGSFGKAVLVKSTE-DGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. UniProt/SwissProt
Match: sp|Q96PY6|NEK1_HUMAN (Serine/threonine-protein kinase Nek1 OS=Homo sapiens OX=9606 GN=NEK1 PE=1 SV=2) HSP 1 Score: 280.026 bits (715), Expect = 2.789e-79 Identity = 129/264 (48.86%), Postives = 185/264 (70.08%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y ++KIG+GS+G+ LVK + D +++V+K+I+ +R S +E E +R E +L +++HPNIVQY++SFEEN SLYI M +CE GDL+ R+ +KG L E QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK +++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YE+ TLKHAF A + L KI+ G P + Y+ +L +L+ + ++P RPS IL FI I F+
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTE-DGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. UniProt/SwissProt
Match: sp|Q6P3R8|NEK5_HUMAN (Serine/threonine-protein kinase Nek5 OS=Homo sapiens OX=9606 GN=NEK5 PE=2 SV=1) HSP 1 Score: 249.595 bits (636), Expect = 3.298e-71 Identity = 119/265 (44.91%), Postives = 173/265 (65.28%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS--IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y IK IGQG++G+ +L K K D K V+K+I+F + +E EA++ E LL+ ++HPNIV + +SF+EN L+I M +C+ GDL R+ ++G L E QI+ W +QI++ L +IH+R ILHRD+K QNIFL+K+ + K+GD GIA+ L + ++A+T IGTPYY+SPE+ + KPYN K+DIWSLGCV YE+ TLKH F N L KI + +++EL +LI + SP RPS IL F+ + I ++
Sbjct: 4 YDVIKAIGQGAFGKAYLAKGKS-DSKHCVIKEINFEKMPIQEKEASKKEVILLEKMKHPNIVAFFNSFQENGRLFIVMEYCDGGDLMKRINRQRGVLFSEDQILGWFVQISLGLKHIHDRKILHRDIKAQNIFLSKNGMVAKLGDFGIARVLNNSMELARTCIGTPYYLSPEICQNKPYNNKTDIWSLGCVLYELCTLKHPFEGNNLQQLVLKICQAHFAPISPGFSRELHSLISQLFQVSPRDRPSINSILKRPFLENLIPKYL 267
BLAST of Serine/threonine-protein kinase Nek4 vs. TrEMBL
Match: A0A267GHX1 (Protein kinase domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig031136g1 PE=3 SV=1) HSP 1 Score: 381.719 bits (979), Expect = 1.062e-122 Identity = 173/274 (63.14%), Postives = 221/274 (80.66%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKP 938
Y IK+IG+GSYGEVWL KH+ D +++VLKKIS +S +E ++A EC+LL L+HPNIV Y+DSFE LYI MA+CE GD+YT+LKE+KG L E+QI+EW +Q+ MAL Y+HERNILHRDLKTQNIFLT+S IIK+GDLGIA+ L+S DMAKT+IGTPYYMSPELF KPYN KSD+W+LGC YEMATLKHAFNA+++++LSYKIL G+TPDMP++Y+ EL+ L+++MLN SPEKRPS +IL + FIR I +F+E ++R P
Sbjct: 4 YQLIKQIGKGSYGEVWLSKHQK-DRRQYVLKKISLVNASQKERKSAEQECKLLSQLKHPNIVGYRDSFELEGILYIVMAYCEGGDVYTKLKEQKGVPLPERQIVEWFVQVAMALQYMHERNILHRDLKTQNIFLTRSKIIKVGDLGIARVLDSSNDMAKTLIGTPYYMSPELFSNKPYNHKSDVWALGCCVYEMATLKHAFNARDFSSLSYKILKGRTPDMPTQYSSELLELMRSMLNLSPEKRPSVNRILRDPFIRRSIQLFLESTRRPGTAP 276
BLAST of Serine/threonine-protein kinase Nek4 vs. TrEMBL
Match: A0A267DKL9 (Protein kinase domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022866g8 PE=3 SV=1) HSP 1 Score: 380.563 bits (976), Expect = 2.818e-122 Identity = 173/274 (63.14%), Postives = 221/274 (80.66%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKP 938
Y IK+IG+GSYGEVWL KH+ D +++VLKKIS +S +E ++A EC+LL L+HPNIV Y+DSFE LYI MA+CE GD+YT+LKE+KG L E+QI+EW +Q+ MAL Y+HERNILHRDLKTQNIFLT+S IIK+GDLGIA+ L+S DMAKT+IGTPYYMSPELF KPYN KSD+W+LGC YEMATLKHAFNA+++++LSYKIL G+TPDMP++Y+ EL+ L+++MLN SPEKRPS +IL + FIR I +F+E ++R P
Sbjct: 4 YQLIKQIGKGSYGEVWLSKHQK-DRRQYVLKKISLVNASQKERKSAEQECKLLSQLKHPNIVGYRDSFELEGILYIVMAYCEGGDVYTKLKEQKGVPLPERQIVEWFVQVAMALQYMHERNILHRDLKTQNIFLTRSKIIKVGDLGIARVLDSSNDMAKTLIGTPYYMSPELFSNKPYNHKSDVWALGCCVYEMATLKHAFNARDFSSLSYKILKGRTPDMPTQYSSELLELMRSMLNLSPEKRPSVNRILRDPFIRRSIQLFLESTRRPGIAP 276
BLAST of Serine/threonine-protein kinase Nek4 vs. TrEMBL
Match: A0A1I8G6F9 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 385.571 bits (989), Expect = 1.063e-121 Identity = 173/274 (63.14%), Postives = 221/274 (80.66%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKP 938
Y IK+IG+GSYGEVWL KH+ D +++VLKKIS +S +E ++A EC+LL L+HPNIV Y+DSFE LYI MA+CE GD+YT+LKE+KG L E+QI+EW +Q+ MAL Y+HERNILHRDLKTQNIFLT+S IIK+GDLGIA+ L+S DMAKT+IGTPYYMSPELF KPYN KSD+W+LGC YEMATLKHAFNA+++++LSYKIL G+TPDMP++Y+ EL+ L+++MLN SPEKRPS +IL + FIR I +F+E ++R P
Sbjct: 5 YQLIKQIGKGSYGEVWLSKHQK-DRRQYVLKKISLVNASQKERKSAEQECKLLSQLKHPNIVGYRDSFELEGILYIVMAYCEGGDVYTKLKEQKGVPLPERQIVEWFVQVAMALQYMHERNILHRDLKTQNIFLTRSKIIKVGDLGIARVLDSSNDMAKTLIGTPYYMSPELFSNKPYNHKSDVWALGCCVYEMATLKHAFNARDFSSLSYKILKGRTPDMPTQYSSELLELMRSMLNLSPEKRPSVNRILRDPFIRRSIQLFLESTRRPGTAP 277
BLAST of Serine/threonine-protein kinase Nek4 vs. TrEMBL
Match: A0A3S5A6U8 (Protein kinase domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS1983 PE=3 SV=1) HSP 1 Score: 379.407 bits (973), Expect = 1.075e-120 Identity = 176/271 (64.94%), Postives = 222/271 (81.92%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRK 926
NY+FIK IG+GSYGEVWL ++ D K++V+KKI +++++E AA LEC+LL L+HPNIVQYKDSFE ++LYIAM+FCE GDLYTRL+ + G L E+Q++EW +QI +AL Y+HERN+LHRDLKTQNIFLT+S IIK+GDLGIA+ LES + MA T+IGTPYYMSPELF KPYN KSDIW+LGCV YEMATLKHAFNAK++ +LSYKIL G+ P+MP +Y+ +LINLI+AMLN PEKRPS +IL + FIR HI +F+E +K K
Sbjct: 28 NYAFIKAIGKGSYGEVWLCQN-GCDKKQYVIKKIYLQKTNEKERNAANLECKLLAQLKHPNIVQYKDSFEFKDNLYIAMSFCEGGDLYTRLRNQNGVFLDERQLVEWFVQIAIALQYMHERNVLHRDLKTQNIFLTRSKIIKVGDLGIARVLESSHSMATTMIGTPYYMSPELFANKPYNHKSDIWALGCVVYEMATLKHAFNAKSFNSLSYKILSGKIPEMPKEYSLDLINLIRAMLNLKPEKRPSARRILSDAFIRSHIVLFLELTKDK 297
BLAST of Serine/threonine-protein kinase Nek4 vs. TrEMBL
Match: A0A1I8HTB5 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 373.244 bits (957), Expect = 3.071e-120 Identity = 169/268 (63.06%), Postives = 216/268 (80.60%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQS 917
+Y +K IG+GSYGEVWL KH D ++FV+KKIS ++ +E ++A EC+LL L+HPNIV YKDSFE LYI MAFCE GDLY+RLKE++G+ L E+Q++EW +QITMAL Y+HERNILHRDLKTQNIFLT+S IIK+GDLGIA+ L+S DMAKT+IGTPYYMSPELF KPYN KSD+W+LGC YEMATLKHAFNA+++ +LS KIL G+ PDMP +Y+ EL+ LI+AML++ P+KRPS +IL + FIR +I MF++ +
Sbjct: 4 DYQLLKAIGKGSYGEVWLAKHL-RDRRQFVVKKISLQSANQKERKSAEQECKLLSQLKHPNIVGYKDSFEAKGILYIVMAFCEGGDLYSRLKEQRGTALSERQVVEWFVQITMALQYMHERNILHRDLKTQNIFLTRSRIIKVGDLGIARVLDSSNDMAKTLIGTPYYMSPELFSNKPYNHKSDVWALGCCVYEMATLKHAFNARDFNSLSNKILQGRMPDMPMQYSAELVALIRAMLSQDPDKRPSVNRILRDRFIRRNIQMFLDAT 270
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Cavefish
Match: nek4 (NIMA related kinase 4 [Source:NCBI gene;Acc:103043005]) HSP 1 Score: 347.436 bits (890), Expect = 2.085e-107 Identity = 172/318 (54.09%), Postives = 226/318 (71.07%), Query Frame = 3
Query: 114 KNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENN-SLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKPCRHRKTSLSKNLPG-------EFHKELYNYQDKSLGNKVSE 1040
++Y FI+ +G+GSYGEV LV+HK D K++V+KK++ SS RE AA E QLL L+HPNIV Y++S+E ++ LYI M FCE GDLY RLK++KG LL E+Q++EW +QI MAL Y+HE++ILHRDLKTQNIFLTK+ IIK+GDLGIA+ LE++ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEM+TLKHAFNAK+ +L+++I+ G+ P MPSKY+ +L LI+ ML K PE RP IL FI+ I +F+E +K K K + S S + P E + N + KS G K E
Sbjct: 2 ESYGFIRVVGKGSYGEVNLVQHKS-DRKQYVIKKLNLRTSSKRERRAAEQEAQLLSQLKHPNIVTYRESWEGDDCQLYIVMGFCEGGDLYHRLKQQKGELLPERQVVEWFVQIAMALQYLHEKHILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMSTLKHAFNAKDMNSLAFRIIEGKLPQMPSKYDPQLGELIKRMLCKKPEDRPDVKHILRQPFIKQQIAIFLEATKEKTAKSRKKVMNSKSNSTPNAAVLQKQENSLQCLNSEPKSRGKKSEE 318
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Cavefish
Match: nek4 (NIMA related kinase 4 [Source:NCBI gene;Acc:103043005]) HSP 1 Score: 347.821 bits (891), Expect = 2.391e-107 Identity = 172/318 (54.09%), Postives = 226/318 (71.07%), Query Frame = 3
Query: 114 KNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENN-SLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKPCRHRKTSLSKNLPG-------EFHKELYNYQDKSLGNKVSE 1040
++Y FI+ +G+GSYGEV LV+HK D K++V+KK++ SS RE AA E QLL L+HPNIV Y++S+E ++ LYI M FCE GDLY RLK++KG LL E+Q++EW +QI MAL Y+HE++ILHRDLKTQNIFLTK+ IIK+GDLGIA+ LE++ DMA T+IGTPYYMSPELF KPYN+KSD+W+LGC YEM+TLKHAFNAK+ +L+++I+ G+ P MPSKY+ +L LI+ ML K PE RP IL FI+ I +F+E +K K K + S S + P E + N + KS G K E
Sbjct: 2 ESYGFIRVVGKGSYGEVNLVQHKS-DRKQYVIKKLNLRTSSKRERRAAEQEAQLLSQLKHPNIVTYRESWEGDDCQLYIVMGFCEGGDLYHRLKQQKGELLPERQVVEWFVQIAMALQYLHEKHILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMSTLKHAFNAKDMNSLAFRIIEGKLPQMPSKYDPQLGELIKRMLCKKPEDRPDVKHILRQPFIKQQIAIFLEATKEKTAKSRKKVMNSKSNSTPNAAVLQKQENSLQCLNSEPKSRGKKSEE 318
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Cavefish
Match: nek1 (NIMA related kinase 1 [Source:NCBI gene;Acc:103028272]) HSP 1 Score: 285.419 bits (729), Expect = 1.117e-81 Identity = 130/264 (49.24%), Postives = 186/264 (70.45%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y +KKIG+GS+G+ LV+ K+ H+ +V+K+I +R S++E + +R E +L ++ HPNIVQYK+SFEE LYI M +CE GDL+ ++ +KG L E+QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK I++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YEM TLKHAF A N L KI+ G P + Y+++L +L+ + ++P +RPS IL F+ IH F+
Sbjct: 4 YEKVKKIGEGSFGKAILVRSKEDGHQ-YVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEEGGCLYIVMDYCEGGDLFKKINNQKGVLFTEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSMHYSQDLRSLLGLLFKRNPRERPSVCTILDKPFLAHRIHRFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Cavefish
Match: nek1 (NIMA related kinase 1 [Source:NCBI gene;Acc:103028272]) HSP 1 Score: 285.034 bits (728), Expect = 1.158e-81 Identity = 130/264 (49.24%), Postives = 186/264 (70.45%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y +KKIG+GS+G+ LV+ K+ H+ +V+K+I +R S++E + +R E +L ++ HPNIVQYK+SFEE LYI M +CE GDL+ ++ +KG L E+QI++W +QI +AL ++H+R ILHRD+K+QNIFLTK I++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSDIW+LGCV YEM TLKHAF A N L KI+ G P + Y+++L +L+ + ++P +RPS IL F+ IH F+
Sbjct: 4 YEKVKKIGEGSFGKAILVRSKEDGHQ-YVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEEGGCLYIVMDYCEGGDLFKKINNQKGVLFTEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSMHYSQDLRSLLGLLFKRNPRERPSVCTILDKPFLAHRIHRFL 266
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Cavefish
Match: ENSAMXT00000049860.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000129.1:61559:73217:-1 gene:ENSAMXG00000043136.1 transcript:ENSAMXT00000049860.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 247.669 bits (631), Expect = 6.124e-71 Identity = 118/306 (38.56%), Postives = 186/306 (60.78%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK--SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHI--HMFVEQSKRKFDKPCRHRKTSLSKNLPGEFHKELYNYQDKSLG 1025
Y +++IG G+YG+ LV+ + + + V+K+IS++R S RE E ++ E LL ++HPNIV + SF E NSLYI M +C+ GDL ++ ++G E+QI++W +QI + L +IH+R +LHRD+K QNIFLT+ S +K+GD GIA+ L + ++A+T +GTPYY+SPE+ E +PYN K+D+WSLGCV YE+ TL+H F + L +I G + +Y+ +L L+ + SP RPS +L + I H+ + + +F HR +++ PG H + +S G
Sbjct: 4 YELVRQIGHGAYGKALLVRERSGNARHCVIKQISWSRMSPREREDSKKEVTLLSKMKHPNIVAFFRSFNERNSLYIVMEYCDGGDLMKKISMQRGQPFKEQQIMDWFVQICLGLKHIHDRKVLHRDIKAQNIFLTQGGSKVKLGDFGIARLLNNTVELARTCVGTPYYLSPEICENRPYNNKTDMWSLGCVLYELCTLRHPFEGSSLRQLIVRICKGYYTPVSQRYSSDLRTLVSQLFKISPRDRPSVNSLLKRPLLHTLISKHLHPQLMEEEFSHTVLHRVKPVAQ--PGPKHSAVSKVSPRSGG 307
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007414.1 (pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1 gene:ENSPMAG00000006689.1 transcript:ENSPMAT00000007414.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 271.552 bits (693), Expect = 2.270e-85 Identity = 128/277 (46.21%), Postives = 186/277 (67.15%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFI--RDHIHMFVEQSKRKFDKPC 941
Y+ + +IG+GS+G+ LVK K H +V+K+I+ +R S +E + AR E +L ++ HPNIV YK+SFEE +LYI M +CE GDL+ R+ +KG L E QI++WL+Q+ +AL ++H+R ILHRD+K+QNIFLTK+ I+++GD GIA+ L S ++A+T IGTPYY+SPE+ E KPYN KSD+W+LGCV YEM TL+HAF A N L KI+ G P + + Y+ EL + + +L + P RP +L + R M EQ + + + C
Sbjct: 4 YTMVSQIGEGSFGKAMLVKSKADGHL-YVIKEINISRMSPKERDEARKEVCVLANMRHPNIVSYKESFEERGNLYIVMDYCEGGDLFKRINAQKGVLFSEDQILDWLVQLCLALKHVHDRKILHRDIKSQNIFLTKAGIVQLGDFGIARVLNSTTELARTCIGTPYYLSPEICENKPYNHKSDMWALGCVLYEMCTLRHAFEAGNMKNLVLKIIRGSFPPVSAHYSSELRSTLSGLLRRDPRARPPVAVLLSRPLLMARAQRLMVSEQVRIEGNSQC 279
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Sea Lamprey
Match: NEK9 (NIMA related kinase 9 [Source:HGNC Symbol;Acc:HGNC:18591]) HSP 1 Score: 205.682 bits (522), Expect = 1.617e-56 Identity = 109/293 (37.20%), Postives = 180/293 (61.43%), Query Frame = 3
Query: 105 PFTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMG-QTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKPCRHRKTSLSKNLP 977
P ++Y ++ +G+G++GE L + ++ D+ V K+I R SD+E ++ E +L LEH NI+ Y + F + N L I + +C G LY ++ ++ G+ EK I+ + Q+ A+ IH ILHRD+KT NIFLTKS +IK+GD GI+K L+S+ MA++ +GTPYYMSPEL YN KSDIW++GCV YE+ TL F+A N L Y+I+ G + ++ +Y+KE+ ++Q L + E+RP+ +L + I+ +E++ + ++P R ++ ++ +P
Sbjct: 31 PEERHYIPVRVLGRGAFGEAALYR-RNEDNSLVVWKEIDLTRLSDKERRDSQNEIDILSLLEHANIISYYNHFLDGNVLLIELEYCNGGTLYEKIIKQHGAFFPEKDIVWYFYQLVSAVEQIHNFKILHRDIKTLNIFLTKSDLIKVGDFGISKILDSRSQMAESYVGTPYYMSPELIRGDKYNTKSDIWAMGCVLYELLTLTRTFDATNPLKLCYEIIHGKKQVEIDDQYSKEIKEVVQKCLQQDAEQRPTAEDLLELSPIKTR-RREMEKNVWRLNEPARRIRSDNAEAVP 321
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000555.1 (pep scaffold:Pmarinus_7.0:GL477093:35206:42652:1 gene:ENSPMAG00000000500.1 transcript:ENSPMAT00000000555.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 170.244 bits (430), Expect = 1.692e-49 Identity = 72/173 (41.62%), Postives = 116/173 (67.05%), Query Frame = 3
Query: 408 LLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALS-----YKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
++ + +++W +Q+ MA+ YIH ILHRD+KT N+FLTK+ +K+GD GI+K +E DMA T +GTPYY+SPE+ + PY+ KSD+W+LGC+ YE+ L+ AF A N L+ YKI+ G+ + Y++++ L+ +L++SPE RPS +L + + + H+ +FV
Sbjct: 4 IITKTNVMQWFVQVCMAVQYIHSVKILHRDIKTSNVFLTKAGTVKVGDFGISKAMEGTLDMAVTCVGTPYYLSPEMCQDLPYSSKSDVWALGCLLYELCALRPAFEASNIIRLTRRSLFYKIMRGEPDPLDESYSRDVRALVALLLSRSPEDRPSASAVLASPYAQRHLRLFV 176
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Sea Lamprey
Match: nek2 (NIMA-related kinase 2 [Source:ZFIN;Acc:ZDB-GENE-040426-752]) HSP 1 Score: 168.318 bits (425), Expect = 8.394e-46 Identity = 99/256 (38.67%), Postives = 141/256 (55.08%), Query Frame = 3
Query: 201 FVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSF--EENNSLYIAMAFCESGDLYTRLKE--RKGSLLCEKQIIEWLIQITMALSYIHERN-----ILHRDLKTQNIFL-TKSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKP 938
V K + + S+ E + E LL+ L+HPNIV+Y D N +LYI M +CE GDL + + R L E+ ++ L Q+ +AL H R +LHRDLK N+FL + +K+GD G+A+ L+ AKT +GTPYYMSPE F + YN KSDIWSLGC+ YE+ L F A N L+ KI G+ +P +Y+ EL +I MLN RPS +I+ + + + EQ++ +KP
Sbjct: 3 LVWKVLDYGTMSEPEKQMLVSEVNLLRELKHPNIVRYFDRIIDRSNTTLYIVMEYCECGDLASLISRCRRDRRFLEEEFVLRVLAQLVLALRECHRRTAGGRTVLHRDLKPANVFLDARHNVKLGDFGLARILQHDTSFAKTFVGTPYYMSPEQFSRMSYNEKSDIWSLGCLLYELCALVPPFTAMNQKQLAEKIKEGKFRRIPYRYSDELNEIISKMLNLKDYLRPSIEQIMQYGLLAEVLDE--EQARAAPEKP 256
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001750.1 (pep scaffold:Pmarinus_7.0:GL481695:1137:12922:1 gene:ENSPMAG00000001576.1 transcript:ENSPMAT00000001750.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 170.629 bits (431), Expect = 2.160e-45 Identity = 85/170 (50.00%), Postives = 115/170 (67.65%), Query Frame = 3
Query: 366 SGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK--SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKIL 869
G L +++R GSLL E I+ + +QI +AL ++H +ILHRDLKT NI L K +++KIGD GI+K L SK A TV+GTP Y+SPEL E KPYN KSDIW+LGC+ YE+A+LK AF A+N AL KI+ G + +Y+ EL L+ ++LN P KRP ++L
Sbjct: 1 GGTLAEYIQKRGGSLLDEDTILHFFVQIALALHHVHSNHILHRDLKTHNILLDKRQTVVKIGDFGISKILSSK-SKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCILYELASLKRAFEAENLPALVLKIMSGNFAPVSDRYSGELRQLVVSLLNLDPSKRPQLSEVL 169
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Yeast
Match: KIN3 (Nonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol [Source:SGD;Acc:S000000071]) HSP 1 Score: 153.295 bits (386), Expect = 9.461e-41 Identity = 105/326 (32.21%), Postives = 155/326 (47.55%), Query Frame = 3
Query: 105 PFTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYK--DSFEENNSLYIAMAFCESGDLYTRLKERKGS--LLCEKQIIEWLIQITMAL------------SYIHERN---------ILHRDLKTQNIFLT----------------------------------------KSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIR 887
P Y +++IG+GS+G V V H K V K I + + +E + EC +L L+H NIV++ D E+ LY+ M +C GDL +K K + EK + L Q+ AL + I++R ++HRDLK NIFL+ + ++K+GD G+AK LE+ A T +GTPYYMSPE+ +PY+ SDIWSLGCV +EM +L F AKNY L KI G+ +P Y++ L +I +M++ + RPST ++L + IR
Sbjct: 20 PPRSEYQVLEEIGRGSFGSVRKVIHIP-TKKLLVRKDIKYGHMNSKERQQLIAECSILSQLKHENIVEFYNWDFDEQKEVLYLYMEYCSRGDLSQMIKHYKQEHKYIPEKIVWGILAQLLTALYKCHYGVELPTLTTIYDRMKPPVKGKNIVIHRDLKPGNIFLSYDDSDYNINEQVDGHEEVNSNYYRDHRVNSGKRGSPMDYSQVVVKLGDFGLAKSLETSIQFATTYVGTPYYMSPEVLMDQPYSPLSDIWSLGCVIFEMCSLHPPFQAKNYLELQTKIKNGKCDTVPEYYSRGLNAIIHSMIDVNLRTRPSTFELLQDIQIR 344
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Yeast
Match: CDC5 (Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rho1p guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; regulates the shape of the nucleus and expansion of the nuclear envelope during mitosis; similar to Xenopus Plx1 and S. pombe Plo1p; human homologs PLK1, PLK3 can each complement yeast cdc5 thermosensitive mutants [Source:SGD;Acc:S000004603]) HSP 1 Score: 134.42 bits (337), Expect = 4.313e-33 Identity = 85/260 (32.69%), Postives = 144/260 (55.38%), Query Frame = 3
Query: 114 KNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFAR-SSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSI-IKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKK--PYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELIN----LIQAMLNKSPEKRPSTGKIL 869
K+Y +G+G + + +K D + F K ++ A S++ + E Q+ KS+ HPNIVQ+ D FE+++++YI + C +G L LK RK +L E ++ + QI A+ Y+H R ++HRDLK NIF + +KIGD G+A L ++ + T+ GTP Y++PE+ K ++F+ DIWSLG + Y + K F A++ + +I + D +K + + LI+ +L+ P +RPS +I+
Sbjct: 80 KDYHRGHFLGEGGFARCFQIK--DDSGEIFAAKTVAKASIKSEKTRKKLLSEIQIHKSMSHPNIVQFIDCFEDDSNVYILLEICPNGSLMELLKRRK--VLTEPEVRFFTTQICGAIKYMHSRRVIHRDLKLGNIFFDSNYNLKIGDFGLAAVLANESERKYTICGTPNYIAPEVLMGKHSGHSFEVDIWSLGVMLYALLIGKPPFQARDVNTIYERI---KCRDFSFPRDKPISDEGKILIRDILSLDPIERPSLTEIM 332
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Yeast
Match: SPS1 (Putative protein serine/threonine kinase; localizes to the nucleus and cytoplasm; required for efficient spore packaging, prospore membrane development and closure and localization of enzymes involved in spore wall synthesis; interacts with and required for Ssp1p phosphorylation and turnover; member of the GCKIII subfamily of STE20 kinases; multiply phosphorylated on S/T residues; interacts with 14-3-3 proteins, Bmh1p and Bmh2p; expressed at the end of meiosis [Source:SGD;Acc:S000002931]) HSP 1 Score: 129.028 bits (323), Expect = 3.860e-32 Identity = 79/265 (29.81%), Postives = 136/265 (51.32%), Query Frame = 3
Query: 105 PFTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLT-KSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELF--EKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRD 890
P +K YS IG+G++G+V+ K D ++ V K+ SD ++E E L L+ P I Y + E+ S++I M +C G LK + L E+++ + ++T+ L Y+HE+ +HRD+K NI L + ++K+GD G++ + S T +GTPY+M+PE+ E YN K+DIWSLG TYE+ + + + + + P + ++ + + L K+P RPS +L F+++
Sbjct: 13 PPSKLYSIQSCIGRGNFGDVY--KAVDRVTQEIVAIKVVNLEHSDEDIELLAQEIFFLAELKSPLITNYIATMLEDVSMWIVMEYCGGGSCSDLLKRSYVNGLPEEKVSFIIHEVTLGLKYLHEQRKIHRDIKAANILLNEEGMVKLGDFGVSGHIRSTLK-RDTFVGTPYWMAPEVVCCEVDGYNEKADIWSLGITTYELLKGLPPLSKYDPMKVMTNLPKRKPPKLQGPFSDAAKDFVAGCLVKTPADRPSAYNLLSFEFVKN 274
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Yeast
Match: CMK1 (Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication [Source:SGD;Acc:S000001910]) HSP 1 Score: 126.331 bits (316), Expect = 1.648e-31 Identity = 84/272 (30.88%), Postives = 141/272 (51.84%), Query Frame = 3
Query: 114 KNYSFIKKIGQGSYGEVWLVKHKDYDHK---KFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLT----KSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTP--------DMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFI 884
K Y F K +G G++G V K+ + K ++KK + + ++EA E +L+ L HPNIV +KD FE + YI + G+L+ R+ +KG E+ + L++I A+ Y+H +NI+HRDLK +N+ +S + + D GIAK+L+S ++ G+ Y++PE+ + + DIWS+G +TY + AF A+ + G+ P D S K+ I ++A LN P KRP+ ++L + +I
Sbjct: 35 KKYVFGKTLGAGTFGVVRQAKNTETGEDVAVKILIKKA--LKGNKVQLEALYDELDILQRLHHPNIVAFKDWFESKDKFYIITQLAKGGELFDRIL-KKGKF-TEEDAVRILVEILSAVKYMHSQNIVHRDLKPENLLYIDKSDESPLVVADFGIAKRLKSDEELLYKPAGSLGYVAPEVLTQDGHGKPCDIWSIGVITYTLLCGYSAFRAERVQDFLDECTTGEYPVKFHRPYWDSVSNKAKQFI--LKA-LNLDPSKRPTAAELLEDPWI 299
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Yeast
Match: CMK2 (Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication [Source:SGD;Acc:S000005376]) HSP 1 Score: 122.865 bits (307), Expect = 2.857e-30 Identity = 77/268 (28.73%), Postives = 132/268 (49.25%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHK---KFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLT----KSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTP---DMP--SKYNKELINLIQAMLNKSPEKRPSTGKILCNNFI 884
NY F + +G GS+G V + + K +LKK + ++ +++ E +L+ L HPNIV +KD FE + YI G+L+ R+ R E +E ++QI A+ Y+H +N++HRDLK +N+ S + I D GIAKQL+ + D+ G+ Y++PE+ + + DIWS+G +TY + F A++ + + P MP + ++ I L +P RP+ ++L + +I
Sbjct: 46 NYIFGRTLGAGSFGVVRQARKLSTNEDVAIKILLKKA--LQGNNVQLQMLYEELSILQKLSHPNIVSFKDWFESKDKFYIVTQLATGGELFDRILSR--GKFTEVDAVEIIVQILGAVEYMHSKNVVHRDLKPENVLYVDKSENSPLVIADFGIAKQLKGEEDLIYKAAGSLGYVAPEVLTQDGHGKPCDIWSIGVITYTLLCGYSPFIAESVEGFMEECTASRYPVTFHMPYWDNISIDVKRFILKALRLNPADRPTATELLDDPWI 309
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Nematostella
Match: EDO49690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG02]) HSP 1 Score: 337.035 bits (863), Expect = 1.230e-110 Identity = 160/272 (58.82%), Postives = 213/272 (78.31%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNS-LYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKF 929
Y+ K IG+GSYGEV+L KHK D K++VLKK+ ++S RE +AA LE +LL L HPNIV Y++SF+++ LYIAM FCE GDLY+RLK +KG L E QI+EW +QI MAL Y+HE++ILHRDLKTQNIFLTKS IIK+GDLGIA+ LES DMA T+IGTPYYMSPELF KPYN KSD+W+LGC YEM TL+HAFNAK+ ++L YKIL G+TP +P +Y+ +L ++I++ML++ P+KRPS ++L + +I+ I +F+E +K ++
Sbjct: 6 YTIGKCIGKGSYGEVFLSKHKK-DKKQYVLKKVDLQKASVRERKAAELEAKLLSQLRHPNIVSYRESFQDDTGFLYIAMNFCEGGDLYSRLKAQKGIPLDENQIVEWFVQIAMALQYMHEKHILHRDLKTQNIFLTKSKIIKVGDLGIARVLESSSDMATTLIGTPYYMSPELFSNKPYNHKSDVWALGCCLYEMCTLRHAFNAKDMSSLVYKILKGKTPPLPKQYSTDLCSIIKSMLDQDPDKRPSASRLLRHPYIKKQIALFLEGTKNRY 276
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Nematostella
Match: EDO49822 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG20]) HSP 1 Score: 279.256 bits (713), Expect = 1.217e-88 Identity = 129/266 (48.50%), Postives = 186/266 (69.92%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQ 914
Y +KKIG+GS+G+ LVK ++ D K +V+K+I+ R RE E +R E ++L L+HPNIV Y++SFEE+ +LYI M +C+ GDLY ++ ++G E Q+++W +QI + L ++H+R ILHRD+K+QNIFLT+ IIK+GD GIA+ L S ++A+T IGTPYY+SPE+ E +PYN KSDIWSLGCV YEM TLKHAF A N L KI+ G P +P +Y+ ++ L+ +L ++P RPS +L NFI+ I F+ +
Sbjct: 4 YVRVKKIGEGSFGKALLVKKRN-DGKHYVIKEINICRMKPREREESRKEVKVLSQLKHPNIVSYQESFEESGNLYIVMDYCDGGDLYKKINGQRGIAFSEDQVMDWFVQICLGLKHVHDRKILHRDIKSQNIFLTRHGIIKMGDFGIARVLHSTVELARTCIGTPYYLSPEIVENRPYNNKSDIWSLGCVLYEMLTLKHAFEAGNMKNLVLKIIRGSYPPIPLRYSADIRMLVAQLLKRNPHDRPSVNTVLKKNFIQKRIEKFLSK 268
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Nematostella
Match: EDO49138 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH03]) HSP 1 Score: 232.646 bits (592), Expect = 4.031e-71 Identity = 114/251 (45.42%), Postives = 163/251 (64.94%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKIL 869
Y I IG+G++G WLV+ + + + LK+++ + A E ++L +L+H NIV+Y+D+FEE YI M + E GDL+T++K+ E+ I+ WL Q+ +AL +IH +NILHRDLKTQNIFLTK ++KIGD GIA+ L+S D A+T +GTPYY+SPE+ +++PYN KSDIW+LGCV YE+ T H F A N T L KIL G P +P Y L +L+ ML +P RPS +++
Sbjct: 4 YQKIDIIGKGTFGSAWLVESRQ-SKRLYALKELNATAMPSEDRHLALNEVKILSTLKHRNIVRYRDAFEETGRFYIVMEYAEGGDLHTKIKQAVEPFSSER-ILNWLAQLLLALKHIHGQNILHRDLKTQNIFLTKDDVVKIGDFGIARILDSTCDHARTTVGTPYYLSPEICQRQPYNNKSDIWALGCVLYELTTRTHPFTADNLTNLVVKILHGNYPPIPRFYGPLLEDLVAVMLKINPADRPSAKQLI 252
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Nematostella
Match: EDO37069 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGU2]) HSP 1 Score: 222.246 bits (565), Expect = 3.654e-67 Identity = 109/267 (40.82%), Postives = 164/267 (61.42%), Query Frame = 3
Query: 108 FTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLK--ERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHM 902
+ Y ++++G GS+G V+LV+ + ++ VLK+I E A E +LL L HPNIV++ DSF + I +CE GDL ++ + G E I+ W IQI +A+ ++HE ILHRDLKT+NIFL +I+K+GD GI++ L DMA T GTPYYMSPE+ + + YN KSDIWSLG V YE+ L+HAF ++ + YKI+ G+ P + KY+ +L +++K P+ RPS +IL ++FI ++I++
Sbjct: 2 YANRYRVVRRLGSGSFGTVFLVEDLKANGERKVLKEIYVGELQPDETVDAMREAKLLSKLNHPNIVKFMDSFMDREYFCIVTEYCEGGDLDDKISAWRKSGKKFDESLILAWFIQIVLAVQHMHEMRILHRDLKTRNIFLKNNIVKLGDFGISRILMGTSDMATTFTGTPYYMSPEVLKHEGYNHKSDIWSLGVVLYELCNLQHAFQGQSLMGVMYKIVEGELPALSDKYSSQLQAFYSRLMDKDPKLRPSANEILKHHFIAENINV 268
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Nematostella
Match: EDO46185 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQY7]) HSP 1 Score: 223.402 bits (568), Expect = 3.211e-63 Identity = 115/263 (43.73%), Postives = 167/263 (63.50%), Query Frame = 3
Query: 114 KNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS--IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHI 896
+NY ++ +G+G+YG V+L + + D+ ++K+I + E ++A E ++L +HPNI++Y DSF + +L I M + + G +Y L++R G L+ E +I+ +QI +AL ++H+ ILHRDLKTQNI L K ++KIGD GI+K L SK A TVIG+P Y+SPEL E KPYN KSD+WSLGCV YE+ TLK AF A N AL KI+ G +P +Y++EL LI ML P KRP +I+ I + +
Sbjct: 2 QNYEKVRIVGRGAYGTVYLCR-RLVDNFLVIIKQIPVEEMTKEERQSALNEVKVLSMFQHPNIIRYYDSFVQEKALMIVMEYAQGGTIYDYLQQRGGKLMDEDEILRLFVQILLALRHVHKGQILHRDLKTQNILLNKKRKVVKIGDFGISKILSSK-SKANTVIGSPCYISPELCEGKPYNQKSDVWSLGCVLYELTTLKRAFEASNLPALVLKIMRGYFSPIPERYSEELRKLILDMLVLDPTKRPGIKEIMAQPVIVNAL 262
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Medaka
Match: nek4 (NIMA related kinase 4 [Source:NCBI gene;Acc:101169778]) HSP 1 Score: 353.599 bits (906), Expect = 1.911e-109 Identity = 165/275 (60.00%), Postives = 213/275 (77.45%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFE-ENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDK 935
+Y FI+ +G+GSYGEV LVKHK D K++V+KK++ + SS RE +A E QLL L HPNIV Y++S+E ++ LYI M FCE GDLY RLK+RKG LL E+Q++EW +QI MAL Y+HERNILHRDLKTQNIFLTK+ IIK+GDLGIA+ LE++ DMA T+IGTPYYMSPELF KPYN KSD+W+LGC YEM+TLKHAFNAK+ +L Y+I+ G+ P MPS+Y+ +L +LI++ML+K PE+RP IL +I+ I MF+E +K K K
Sbjct: 3 SYIFIRVVGKGSYGEVNLVKHK-TDRKQYVIKKLNLSTSSKRERRSAEQEAQLLSQLRHPNIVTYRESWEGDDRQLYIVMGFCEGGDLYHRLKQRKGELLPERQVVEWFVQIAMALQYLHERNILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNHKSDVWALGCCVYEMSTLKHAFNAKDMNSLVYRIVEGKLPQMPSRYDPQLGDLIKSMLSKKPEERPDVKLILRQPYIKRQIAMFLEATKEKTAK 276
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Medaka
Match: ENSORLT00000000545.2 (pep primary_assembly:ASM223467v1:4:1040495:1044496:1 gene:ENSORLG00000000439.2 transcript:ENSORLT00000000545.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 250.751 bits (639), Expect = 1.631e-77 Identity = 124/264 (46.97%), Postives = 177/264 (67.05%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
Y +K+IG+GS+G+ LV K+ D +++V+K+I + S +E +R E +L ++ HPNIVQYK+SFE LYI M +CE GDL+ ++ +KG L E QI++W +QI +AL ++H R ILHRD+K+QNIFLTK +++GD GIA+ L + ++A+T IGTPYY+SPE+ E KPYN K DIW+LGCV YEM TLKHAF A N L KI+ G P + Y+ EL +L+ + ++P +RPS IL F+ I F+
Sbjct: 4 YENVKQIGEGSFGKAILVNSKE-DGRQYVIKEIGISGMSTKERRESRKEVSVLANMSHPNIVQYKESFE-GGCLYIVMDYCEGGDLFKKINSQKGVLFSEDQILDWFVQICLALKHVHGRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNT-VELARTCIGTPYYLSPEICENKPYNNK-DIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSPELRSLLAQLFRRNPRERPSVSSILEKPFLSCRIEKFL 263
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Medaka
Match: ENSORLT00000034147.1 (serine/threonine-protein kinase Nek5 [Source:NCBI gene;Acc:101162253]) HSP 1 Score: 234.187 bits (596), Expect = 2.862e-66 Identity = 107/263 (40.68%), Postives = 168/263 (63.88%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHK-DYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSIIK--IGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHI 896
+Y + +IG+G++G+ +LV+ K + V+K+I+ + S +E E++R E LL + HPNIV + SF E SL+I M FC+ GDL ++ ++G E++I++ +QI + L +IH++ +LHRD+K QNIFLT +K +GD GIA+ L + D+A+T +GTPYY+SPE+ E +PYN K+D+WSLGCV YE+ TL+H F + L KI G+ +P++Y EL L+ + +P RPS +L F++ HI
Sbjct: 3 DYEVLWQIGEGAFGKAFLVRSKTGGGDTQCVIKQINLQKMSAKERESSRKEVTLLSRMRHPNIVSFISSFLEKGSLFIVMEFCDGGDLLKKINMQRGVPFREEEILDLFVQICLGLKHIHDKKVLHRDIKCQNIFLTNGGMKAKLGDFGIARMLNNSMDLARTCVGTPYYLSPEICESRPYNNKTDVWSLGCVLYELCTLRHPFEGSSLRQLLGKICRGRYAPVPTRYTHELRLLVNQLFKVNPRDRPSISSVLQRPFLQKHI 265
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Medaka
Match: nek8 (NIMA related kinase 8 [Source:NCBI gene;Acc:101161178]) HSP 1 Score: 224.557 bits (571), Expect = 4.589e-63 Identity = 129/293 (44.03%), Postives = 180/293 (61.43%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS--IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFI-RDHIHMFVEQSK---RKFDKPCRHRKTSLSKNLPG 980
Y IK +G+G++G V L + + D +LK+I + S E AA+ ECQ+LK L HPNI++Y ++F E+ +L IAM + G L +++R SLL E I+ + +QI +AL ++H + ILHRDLKTQNI L K I+KIGD GI+K L SK A TV+GTP Y+SPEL E KPYN KSDIW+LGCV YE+A+LK AF A N AL KI+ G + +Y+ EL LI MLN P KRP +I+ R ++++ + R+ +KP +++ LPG
Sbjct: 4 YEKIKVVGRGAFGIVHLCRRRS-DGAFVILKEIPVEQMSRDERLAAQNECQVLKLLNHPNIIEYYENFLEDKALMIAMEYAPGGTLAEYIQKRCNSLLDEDTILHFFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSK-SKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILNMLNLDPSKRPQLNEIMALPICTRPLLNLYTDVGNVKMRRIEKP----LSAVQLGLPG 290
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Medaka
Match: ENSORLT00000027525.1 (NIMA related kinase 3 [Source:NCBI gene;Acc:101171427]) HSP 1 Score: 209.92 bits (533), Expect = 9.708e-60 Identity = 99/264 (37.50%), Postives = 163/264 (61.74%), Query Frame = 3
Query: 120 YSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLT-KSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNN----FIRDHI 896
Y+ +K +G+GS+G LV+ +K+V+K+I ++ + + +R E LL ++HPNIV ++++FE + L I M +C GDL R++++K + C + I+ W Q+ +IH++ +LHRDLK++NIFLT +K+GD G A L S A +GTPYY++PE+++ KPYN KSD+WSLGCV YE+ L+H F A ++ +L K+ G P +P EL++L++ + +P+ RPS IL ++ +R H+
Sbjct: 4 YTQLKVVGEGSFGRAVLVQWIS-TQEKYVVKEIQLPKNESK-LRDSRKEAILLSRMKHPNIVAFREAFEMTDLLCIVMEYCSGGDLLQRIQQQKATNFCTENILRWFAQMCAGAQHIHDKRVLHRDLKSKNIFLTGDGSVKLGDFGSACVLNSSKAYAHAYVGTPYYVAPEIWDNKPYNNKSDVWSLGCVLYELCALQHPFQASSWKSLILKVCRGAYPPLPRHLPYELLHLMKQIFKTNPKDRPSVQTILTSHRVSKLLRSHL 265
BLAST of Serine/threonine-protein kinase Nek4 vs. Planmine SMEST
Match: SMESG000005635.1 (SMESG000005635.1) HSP 1 Score: 1159.82 bits (2999), Expect = 0.000e+0 Identity = 628/635 (98.90%), Postives = 632/635 (99.53%), Query Frame = 3
Query: 99 MVPFTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKFDKPCRHRKTSLSKNLPGEFHKELYNYQDKSLGNKVSEVSDENNDKLKTTVDTSTLKNHXXXXXXXXXXXXIXXXXXXXXXXXXXXXXXENQVDSRKSSKTDLEIEFQKGLRNSEDKVVAHQYKQNENLMRSRSFSSDPVAPKVGQAEVPEESSPNKVAREKRRHIKREQGFQTLRNPSAICGNNFDIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGNGLPLPELRRSDNAEDMQNEEVTSIDVLQVVEKMNETLNLNEKFNNIKDSILLYKIQKEPNNVEPIEDEQETLDRSKFMNKRYQEIRKELMTAVGYKNLALCFDILRDLNLNHEEKIREILGEDYDRYCGKVIWLKMIEEA 2003
M PFTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRK+DKPCRHRKTSLSKNLPGEFHKELYNYQDKSLGNKVSEVSDENNDKLKTTVDTSTLKN+DDEYDDNDDVVVIHSSNDSHSKKSSNSSSKENQVDSRKSSKTDLEIEFQKGLRNSEDKVVAHQYKQNENL+RSRSFSSDPVAPKVGQAEVPEESSPNKVAREKRR +KREQGFQTLRNPSAICGNNFDINDEKDNKADEN SNHNRNRRRKNNSFNQNGNGLPLPELRRSDNAEDMQNEEVTSIDVLQVVEKMNETLNLNEKFNNIKDSILLYKIQKEPNNVEPIEDEQETLDRSKFMNKRYQEIRKELMTAVGYKNLALCFDILRDLNLNHEEKIREILGEDYDRYCGKVIWLKMIEEA
Sbjct: 1 MTPFTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKSIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFVEQSKRKYDKPCRHRKTSLSKNLPGEFHKELYNYQDKSLGNKVSEVSDENNDKLKTTVDTSTLKNNDDEYDDNDDVVVIHSSNDSHSKKSSNSSSKENQVDSRKSSKTDLEIEFQKGLRNSEDKVVAHQYKQNENLVRSRSFSSDPVAPKVGQAEVPEESSPNKVAREKRRQMKREQGFQTLRNPSAICGNNFDINDEKDNKADENGSNHNRNRRRKNNSFNQNGNGLPLPELRRSDNAEDMQNEEVTSIDVLQVVEKMNETLNLNEKFNNIKDSILLYKIQKEPNNVEPIEDEQETLDRSKFMNKRYQEIRKELMTAVGYKNLALCFDILRDLNLNHEEKIREILGEDYDRYCGKVIWLKMIEEA 635
BLAST of Serine/threonine-protein kinase Nek4 vs. Planmine SMEST
Match: SMESG000020466.1 (SMESG000020466.1) HSP 1 Score: 240.736 bits (613), Expect = 4.572e-67 Identity = 118/265 (44.53%), Postives = 171/265 (64.53%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
++ + KIGQG++G WLV+ K D KK+VLK+I + ++E AR E LL + HPNIV Y +SFEE +I M +CE GDLY ++K + G L E Q++++ IQI +AL +IH++ ILHRD+K QNIFLTK + +K+GD GIAK ++S + KT +GTP+Y SPE+ E PYN KSDIWSLGC+ YE+ +L +AF ++ T L+ +I +PS ++ L L+ M + P++RP+ IL FI I F+
Sbjct: 6 DFKRLNKIGQGAFGIAWLVESK-IDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 269
BLAST of Serine/threonine-protein kinase Nek4 vs. Planmine SMEST
Match: SMESG000020466.1 (SMESG000020466.1) HSP 1 Score: 240.736 bits (613), Expect = 4.811e-67 Identity = 118/265 (44.53%), Postives = 171/265 (64.53%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
++ + KIGQG++G WLV+ K D KK+VLK+I + ++E AR E LL + HPNIV Y +SFEE +I M +CE GDLY ++K + G L E Q++++ IQI +AL +IH++ ILHRD+K QNIFLTK + +K+GD GIAK ++S + KT +GTP+Y SPE+ E PYN KSDIWSLGC+ YE+ +L +AF ++ T L+ +I +PS ++ L L+ M + P++RP+ IL FI I F+
Sbjct: 6 DFKRLNKIGQGAFGIAWLVESK-IDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 269
BLAST of Serine/threonine-protein kinase Nek4 vs. Planmine SMEST
Match: SMESG000020466.1 (SMESG000020466.1) HSP 1 Score: 240.736 bits (613), Expect = 4.925e-67 Identity = 118/265 (44.53%), Postives = 171/265 (64.53%), Query Frame = 3
Query: 117 NYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 908
++ + KIGQG++G WLV+ K D KK+VLK+I + ++E AR E LL + HPNIV Y +SFEE +I M +CE GDLY ++K + G L E Q++++ IQI +AL +IH++ ILHRD+K QNIFLTK + +K+GD GIAK ++S + KT +GTP+Y SPE+ E PYN KSDIWSLGC+ YE+ +L +AF ++ T L+ +I +PS ++ L L+ M + P++RP+ IL FI I F+
Sbjct: 6 DFKRLNKIGQGAFGIAWLVESK-IDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 269
BLAST of Serine/threonine-protein kinase Nek4 vs. Planmine SMEST
Match: SMESG000043302.1 (SMESG000043302.1) HSP 1 Score: 214.157 bits (544), Expect = 3.522e-60 Identity = 118/248 (47.58%), Postives = 156/248 (62.90%), Query Frame = 3
Query: 129 IKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTKS-IIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKIL 869
I+ +G+G+YG L + KD D +LK+I+ S E + A E ++L L+HPNIV Y DSFEE+ L I M + ++G L L ++ L EK+I+E QI A+ YIHE ILHRDLKT NIFLTK IIKIGD GI+K L + ++ A +VIGTPYY+SPE+ + K YN KSD+W+LGC+ YEM L F N AL KIL GQ + Y+ EL LI ++L K+P+ R ST IL
Sbjct: 63 IRTVGKGAYGTAVLYRKKD-DDSLVILKEINMHDLSATERQLAMNEVKVLSQLDHPNIVAYFDSFEEDGVLMIEMEYADNGTLAQFLSNQEKPLE-EKKILEMFFQIVSAIHYIHEHKILHRDLKTVNIFLTKEEIIKIGDFGISKMLSTTHEGANSVIGTPYYISPEMCQGKLYNEKSDVWALGCILYEMTCLHKTFEGSNLPALVNKILKGQFEPVKGSYSDELKELISSLLEKNPDDRLSTEVIL 308 The following BLAST results are available for this feature:
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase Nek4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016820 ID=SMED30016820|Name=Serine/threonine-protein kinase Nek4|organism=Schmidtea mediterranea sexual|type=transcript|length=2228bpback to top protein sequence of SMED30016820-orf-1 >SMED30016820-orf-1 ID=SMED30016820-orf-1|Name=SMED30016820-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=636bp MVPFTKNYSFIKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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