Vacuolar protein sorting 13 homolog D
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016802 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Human
Match: VPS13D (vacuolar protein sorting 13 homolog D [Source:HGNC Symbol;Acc:HGNC:23595]) HSP 1 Score: 619.002 bits (1595), Expect = 6.055e-177 Identity = 475/1581 (30.04%), Postives = 748/1581 (47.31%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNS-KGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERNVAFCDCRK---------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCG--------------DENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS---CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGM----KCYINASCKNAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V +C IDDC+D DVPL ++ F+ + + S +G LS DYYN GW P IEPWP ++ W K ++ L+ +I+S L+ +T + W ++ + + + + FVPF + N GC + + L+ P + ++ G + ++W V T + F+ E AR K+R+ +H H++++++ GW + PV+VDK+G F+R+ D + + + +R+V + +GS + I +RS L + N L P+E + V+ + P +PL + +Q RP + F +PI W V ++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K +R+G M P W + SL+ +RA V Q NRP L+YNIG++++ GRG++I+T MVI RYLLDN+SS L F Q Q I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITL+GA YR+ F+D D LP P++++N + + V F Q+ V+E R + P + + YA DEPTLPP I L ++G S C ++N + LYY+NF+YIA +F + LV DV+P ++V L +K RSQLW + L HEGS PH+P K RS + +LD+ LA + + + ++L K D RR Q W F EG + G+ K ++ A V G +T E P+ + F RPGSG + + V+ DGPTR +++ D + DE +++ +L + T +E+ +EG G+ LIN +PEEL++A ++V + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC + LLP
Sbjct: 2709 ESVCICFIDDCMDCDVPLAELTFSRLNFLQRVRTSPEGYAHFTLSGDYYNRALSGWEPFIEPWPCSVSWQQQAASRLHPPRLKLEAKAKPRLDINITSVLIDQYVSTKESWMADYCKDDKDIESAKSEDWMGSSVDPPCFGQTEVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPGVVPEGNGTFLDDTHNVSEWREV--LTGEEIPFEFE--ARGKLRHRH--THDLRIHQLQVRVNGWEQVSPVSVDKVGTFFRYAAPDKNSSSSTIGSPSSRTNIIHPQVYFSSLPPVRVVFAVTMEGSARKVITVRSALIVRNRLETPMELRLDSPSAPDKPVVLPAIMPGDSFAVPLHLTSWRLQARP-----------KGLGVFFCKAPIH-------WTNV------VKTAEISSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALHTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIVCRAEGSLKIFISAPYWLINKTGLPLIFRQDN-AKTDAAGQFEEHELARSLSPLLFCYADKEQPNLCTMRIGRGIHPEGM----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGRYIDTCMVIFAPRYLLDNKSSHKLAFAQREFARGQGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCIWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLRGATYRISFSDTDQLPPPFRIDNFSKVPVVFTQHGVAEPRLRTE--VKPMTSLDYAWDEPTLPPFITLTVKGAGSSEINC-NMNDFQDNRQLYYENFIYIAATYTFSGLQEGTGRPVASNKAITCAELVLDVSPKTQRVILKKKEPGKRSQLWRMTGTGMLAHEGSSVPHNPNKPSAARSTEGSAILDIAGLA---AVTDNRYEPLMLRKPDRRRSTTQTWSFREGKLTCGLHGLVVQAKGGLSGLFDGAEVVLGPDTSMELLGPVPPEQQFINQKMRPGSGMLSIRVIPDGPTRALQITDFCHRKSSRSYEVDELPVTEQELQKLKNPDTEQELEVLVRLEGGIGLSLINKVPEELVFASLTGINVHYTQLATSHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMITAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENLHEKTAEQGGTPIRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHAATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGLGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGPRTQAQRVRKPRCCTGPQGLLP 4216 HSP 2 Score: 295.819 bits (756), Expect = 6.971e-79 Identity = 298/1256 (23.73%), Postives = 552/1256 (43.95%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAA------LRERARNLVKYVQSY----EDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRK---SRDLLQNAVNIP-----APQQSGWWGYL---------WGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDI--TELLNE--VDFEAVNFFT-RDKIFLRGEFSLEGIKLKLL-KDTEIP------ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-------------NPDYK----NDSFRYVVSSREHSKV-----PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEK---GS---SDIRWWVSLKISAPKIILP-NFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPV-------------TNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPE--------INQNYKSVSI-------SFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLS--------ESIGDP-------------------------------IIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKE-----------FDVSIACKAVSILLIKISSNLASENRFA--VSVGRISLSQMNLLM 3274
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+G IG+VTL+IP+ R W I I +++I P EKE E KK LL ++E KW+ +Q+G++ W S AS+ RI+ N+++++++VH+R+ED T PS FA G+ I S +++ E K + KQ+D++ SIY D C ++ ++ ++ + + E ++ P+ ++KRN + PL H PRI +Q+E + + LSQ QY+ + E ER +RQ +++P+V++ ++ +EWW FA+ + I + + RAR+ V Y Y + LL+ D + E +E + + L LRE+ + ++ + L + + P AP+ G G L WG + +PE ++E L + EQ + ++I TE + D +N +T RD +F + L+ + LL K+ P +++ ++ + ES P+ +S + + L LF+RDL NP + + SF +S + S P G +F + +E NP S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ L++ G RW V L ISAP++I P +F+ K P V++DLGR+ ++N D +S +A E E+ +++ TP ++P + S +++ + + V T +Y+ YS+ ++ +VG NW ++ T ++E FN L L+++ + + P ++ N+ L I I++ K+ K C +T + + ++ + +L S + Q+ + V + F ++ + +ES R ++ L + T + +S+ + L+L D G+ D ++ASHK L D P + P +I+ Y+ +S + S ++ + +I ++LD+I N ET+ EL+ F++ F + + Q T F+E +V++ +++LL++ + +A+ ++ ++ I +++N+ M
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSVALLKGAVELENLPLKKDALKEL-ELPFEVKAGFIGKVTLQIPFYRPHVDPWVISISSLHLIGAPEKIQDFNDEKEKLLERERKKALLQALEEKWKNDRQQKGES------YWYSVTASVVTRIVENIELKIQDVHLRFEDGVTNPSHPFAFGICIKNVSMQNAVNEPVQKL-------MRKKQLDVAEFSIYWDVDCTLLGDLPQMELQEAMARSM-ESRSHHYVLEPVFASALLKRNCSKKPLRSRHSPRIDCDIQLETIPLKLSQLQYRQIMEFLKELERKERQVKFRRWKPKVAISKNCREWWYFALNANLYEIREQRKRCTWDFMLHRARDAVSYTDKYFNKLKGGLLSTD---DKEEMCRIEEEQSFEELKILRELVHDRFHKQEELAESLREPQFDSPGACPGAPEPGGGSGMLQYLQSWFPGWGGWYGQQTPE-----------GNVVEGL---SAEQQEQWIPEEILGTEEFFDPTADASCMNTYTKRDHVFAKLNLQLQRGTVTLLHKEQGTPQMNESAFMQLEFSDVKLLAESLPRRNSSLLSVRLGGLFLRDLATEGTMFPLLVFPNPQKEVGRVSQSFGLQTTSADRSDHYPAADPDGPVFEMLYERNPAHS----HFERRLNVSTRPLNIIYNPQAIKKVADFFYKGKVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTLDRLLVGDFIEESKRWTVRLDISAPQVIFPDDFKFKN--PVLVVVDLGRMLLTNTQDNSRRKSRDGSASE----ETQFSDDEYKTPLATPPNTPPPESSSSNGEKTPPFSGVEFSEEQLQAHLMSTKMYERYSLSFMDLQIMVGRVKDNWKHVQDIDVGPTH------VVEKFNVHLQLERRLIYTSDPKYPGAVLSGNLPDLKIHINEDKISALKNCFALLTTPEMKTSDTQIKEKIFPQEEQRGSLQDSVMNLTQSIVLLEQHTREVLVESQLLLAEFKVNCMQLGVESNGRYISVLKVFGTNAHFVKRPYDAEVSLTVHGLLLVDTMQTYGADFDLLMASHKNLSFDIPTGSLRDSRAQSPVSGPNVAHLTDGATLNDRSATSVSLDKILTKEQESLIKLEYQFVSSECPSMNLDSTLQVISLQVNNLDIILNPETIVELIGFLQKSFPKEKDDLSPQPLMTDFERSFREQGTYQSTYEQNTEVAVEIHRLNLLLLR-TVGMANREKYGRKIATASIGGTKVNVSM 1207 HSP 3 Score: 172.555 bits (436), Expect = 1.230e-41 Identity = 147/475 (30.95%), Postives = 236/475 (49.68%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILK-----DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLM--SRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRL-NVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSL--ATDDENRFDQHVAFYIQSKPQSRSISFQ---PVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
CLLD + +DL +MD+++A R K GDL F +R L+E C L L V RNL+ ISHT PD I+G LSSV S+ + +Y+ IRG+L +NLGE + ++ Y Q DP +HT L+G Y M + + +V L L R +G +L R +F + KL YESFS+ +K+I LV + D RY+ + + NVF I P KN+ ++ I Q+ + + S D + F V LN ++ +IFDW+ + +++ T P I T +R + + +S ++ + PV +H E+ +NV + F ++E+ S T+A+IL +YK R +R PFS S+ + SC + ++ LSI++PV+I ++ L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 2151 CLLDCVVVDLQDMDIFAAERHPREYSKAPEDSSGDLIFPSYFVRQTGGSLLTEPCRLKLQVERNLDKEISHTVPDISIHGNLSSVHCSLDLYKYKLIRGLLENNLGEPIEEFMRPYDLQ----DPRIHTVLSGEVYTCMCFLIDMVNVSLELKDPKRKEGAG-----SLARFDFKKCKLLYESFSNQTKSINLVSHSMMAFDTRYAGQKTSPGMTNVFSCIFQPAKNSSTTQGSI---QIELHFRSTKDSSCFTVVLNNLRVFLIFDWLLLVHDFLHT-PSDIKKQNHVTPSRHRNSSSESAIVPKTVKSGVVTKRSSLPVSNERHL-----------EVKVNVTGTEFVVIEDVSCFDTNAIILKGTTVLTYKPRFVDR--PFSGSLFGIEVFSCRLGNEHDTALSIVDPVQIQMELVGN-SSYQNSSGLMDAFNSEDFPPV-LEIQLQALDIRLSYNDVQLFLAIAKSIPEQA 2597 HSP 4 Score: 157.532 bits (397), Expect = 4.206e-37 Identity = 149/534 (27.90%), Postives = 262/534 (49.06%), Query Frame = 2
Query: 3980 KAVYEQVLKSMDNLVY-DDEDEIPK-------------PFIEFEDENMQEDQNPDPSENEIPIVLQSN-----------------ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSS---SNIGQENLQEKS------VSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNS-VDIQNCAAFELKLPKNTV-KSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVK----DLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF 5443
K VYEQVL+++DNLVY +D ++ P P + +++ +N S + + Q + IS+++++L D ++ ++ L Q +EF + + I++A+ + + +L+ K L+ S + S++ Q+ +S V PD+ L S + P K++K + + + + + PK V KS + L I I L+DKK FS+ + ++R +D DFN L I+++ WV++LDFFG+GS D ++ +L K+ P + + +++ +NTKL++ + SL+L T + +N VS + L+ +G++ L GS+ L DL+ G Y +R F S ++ ++ F FKYG PDP L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V AQR +R+ + I AP+LL+P S+ S+++++ +LGK + N+F F
Sbjct: 1539 KHVYEQVLQTLDNLVYSEDLNKYPASATSSPCPDSPLPPLSTCGESSVERKENGLFSHSSLSNTSQKSLSVKEVKSFTQIQATFCISELQVQLSGDLTLGAQG-LVSLKFQDFEVEFSKDHPQTLSIQIALHSLLMEDLLEKNPDSKYKNLMVSRGAPKPSSLAQKEYLSQSCPSVSNVEYPDMPRSLPSHMEEAPNVFQLYQRPTSASRKKQKEVQDKDYPLTPPPSPTVDEPKILVGKSKFDDSLVHINIFLVDKKHPEFSSSYNRVNRSIDVDFNCLDVLITLQTWVVILDFFGIGST-ADNHAMRLPPEGILHNVKLEP--HASMESGLQDPVNTKLDLKVHSLSLVLNKTTSELAKAN---VSKLVAHLEMI--EGDLALQGSIGSLSLSDLTCHGEFYRER-FTTSGEEALI-FQTFKYGRPDPLLRREHDIRVSLRMA-SVQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAIEGQTVRDQAQRCSRVLLDIEAGAPVLLIPESSRSNNLIVANLGKLKVKNKFLF 2060
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Human
Match: VPS13D (vacuolar protein sorting 13 homolog D [Source:HGNC Symbol;Acc:HGNC:23595]) HSP 1 Score: 611.683 bits (1576), Expect = 3.647e-175 Identity = 477/1605 (29.72%), Postives = 749/1605 (46.67%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNS-KGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--------------------------QERNVAFCDCRK-------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCG--------------DENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS---CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGM----KCYINASCKNAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V +C IDDC+D DVPL ++ F+ + + S +G LS DYYN GW P IEPWP ++ W K ++ L+ +I+S L+ +T + W ++ Q + + R + FVPF + N GC + + L+ P + ++ G + ++W V T + F+ E AR K+R+ +H H++++++ GW + PV+VDK+G F+R+ D + + + +R+V + +GS + I +RS L + N L P+E + V+ + P +PL + +Q RP + F +PI W V ++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K +R+G M P W + SL+ +RA V Q NRP L+YNIG++++ GRG++I+T MVI RYLLDN+SS L F Q Q I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITL+GA YR+ F+D D LP P++++N + + V F Q+ V+E R + P + + YA DEPTLPP I L ++G S C ++N + LYY+NF+YIA +F + LV DV+P ++V L +K RSQLW + L HEGS PH+P K RS + +LD+ LA + + + ++L K D RR Q W F EG + G+ K ++ A V G +T E P+ + F RPGSG + + V+ DGPTR +++ D + DE +++ +L + T +E+ +EG G+ LIN +PEEL++A ++V + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC + LLP
Sbjct: 1532 ESVCICFIDDCMDCDVPLAELTFSRLNFLQRVRTSPEGYAHFTLSGDYYNRALSGWEPFIEPWPCSVSWQQQAASRLHPPRLKLEAKAKPRLDINITSVLIDQYVSTKESWMADYCKDDKDIESAKSEDWMGSSVDPPCFGQSLPLVYLRTRSTASLTNLEHQIYAREVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPGVVPEGNGTFLDDTHNVSEWREV--LTGEEIPFEFE--ARGKLRHRH--THDLRIHQLQVRVNGWEQVSPVSVDKVGTFFRYAAPDKNSSSSTIGSPSSRTNIIHPQVYFSSLPPVRVVFAVTMEGSARKVITVRSALIVRNRLETPMELRLDSPSAPDKPVVLPAIMPGDSFAVPLHLTSWRLQARP-----------KGLGVFFCKAPIH-------WTNV------VKTAEISSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALHTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIVCRAEGSLKIFISAPYWLINKTGLPLIFRQD-NAKTDAAGQFEEHELARSLSPLLFCYADKEQPNLCTMRIGRGIHPEGM----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGRYIDTCMVIFAPRYLLDNKSSHKLAFAQREFARGQGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCIWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLRGATYRISFSDTDQLPPPFRIDNFSKVPVVFTQHGVAEPRLRTE--VKPMTSLDYAWDEPTLPPFITLTVKGAGSSEINC-NMNDFQDNRQLYYENFIYIAATYTFSGLQEGTGRPVASNKAITCAELVLDVSPKTQRVILKKKEPGKRSQLWRMTGTGMLAHEGSSVPHNPNKPSAARSTEGSAILDIAGLA---AVTDNRYEPLMLRKPDRRRSTTQTWSFREGKLTCGLHGLVVQAKGGLSGLFDGAEVVLGPDTSMELLGPVPPEQQFINQKMRPGSGMLSIRVIPDGPTRALQITDFCHRKSSRSYEVDELPVTEQELQKLKNPDTEQELEVLVRLEGGIGLSLINKVPEELVFASLTGINVHYTQLATSHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMITAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENLHEKTAEQGGTPIRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHAATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGLGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGPRTQAQRVRKPRCCTGPQGLLP 3063 HSP 2 Score: 172.94 bits (437), Expect = 8.723e-42 Identity = 147/475 (30.95%), Postives = 237/475 (49.89%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILK-----DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLM--SRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRL-NVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGI--VSLATDDENRFDQHVAFYIQSKPQSRSISFQ---PVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
CLLD + +DL +MD+++A R K GDL F +R L+E C L L V RNL+ ISHT PD I+G LSSV S+ + +Y+ IRG+L +NLGE + ++ Y Q DP +HT L+G Y M + + +V L L R +G +L R +F + KL YESFS+ +K+I LV + D RY+ + + NVF I P KN+ ++ I Q+ + + S D + F V LN ++ +IFDW+ + +++ T P I + T +R + + +S ++ + PV +H E+ +NV + F ++E+ S T+A+IL +YK R +R PFS S+ + SC + ++ LSI++PV+I ++ L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 974 CLLDCVVVDLQDMDIFAAERHPREYSKAPEDSSGDLIFPSYFVRQTGGSLLTEPCRLKLQVERNLDKEISHTVPDISIHGNLSSVHCSLDLYKYKLIRGLLENNLGEPIEEFMRPYDLQ----DPRIHTVLSGEVYTCMCFLIDMVNVSLELKDPKRKEGAG-----SLARFDFKKCKLLYESFSNQTKSINLVSHSMMAFDTRYAGQKTSPGMTNVFSCIFQPAKNSSTTQGSI---QIELHFRSTKDSSCFTVVLNNLRVFLIFDWLLLVHDFLHT-PSDIKKQNHVTPSRHRNSSSESAIVPKTVKSGVVTKRSSLPVSNERHL-----------EVKVNVTGTEFVVIEDVSCFDTNAIILKGTTVLTYKPRFVDR--PFSGSLFGIEVFSCRLGNEHDTALSIVDPVQIQMELVGN-SSYQNSSGLMDAFNSEDFPPV-LEIQLQALDIRLSYNDVQLFLAIAKSIPEQA 1420 HSP 3 Score: 158.688 bits (400), Expect = 2.172e-37 Identity = 149/534 (27.90%), Postives = 262/534 (49.06%), Query Frame = 2
Query: 3980 KAVYEQVLKSMDNLVY-DDEDEIPK-------------PFIEFEDENMQEDQNPDPSENEIPIVLQSN-----------------ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSS---SNIGQENLQEKS------VSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNS-VDIQNCAAFELKLPKNTV-KSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVK----DLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF 5443
K VYEQVL+++DNLVY +D ++ P P + +++ +N S + + Q + IS+++++L D ++ ++ L Q +EF + + I++A+ + + +L+ K L+ S + S++ Q+ +S V PD+ L S + P K++K + + + + + PK V KS + L I I L+DKK FS+ + ++R +D DFN L I+++ WV++LDFFG+GS D ++ +L K+ P + + +++ +NTKL++ + SL+L T + +N VS + L+ +G++ L GS+ L DL+ G Y +R F S ++ ++ F FKYG PDP L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V AQR +R+ + I AP+LL+P S+ S+++++ +LGK + N+F F
Sbjct: 362 KHVYEQVLQTLDNLVYSEDLNKYPASATSSPCPDSPLPPLSTCGESSVERKENGLFSHSSLSNTSQKSLSVKEVKSFTQIQATFCISELQVQLSGDLTLGAQG-LVSLKFQDFEVEFSKDHPQTLSIQIALHSLLMEDLLEKNPDSKYKNLMVSRGAPKPSSLAQKEYLSQSCPSVSNVEYPDMPRSLPSHMEEAPNVFQLYQRPTSASRKKQKEVQDKDYPLTPPPSPTVDEPKILVGKSKFDDSLVHINIFLVDKKHPEFSSSYNRVNRSIDVDFNCLDVLITLQTWVVILDFFGIGST-ADNHAMRLPPEGILHNVKLEP--HASMESGLQDPVNTKLDLKVHSLSLVLNKTTSELAKAN---VSKLVAHLEMI--EGDLALQGSIGSLSLSDLTCHGEFYRER-FTTSGEEALI-FQTFKYGRPDPLLRREHDIRVSLRMA-SVQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAIEGQTVRDQAQRCSRVLLDIEAGAPVLLIPESSRSNNLIVANLGKLKVKNKFLF 883
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Human
Match: VPS13D (vacuolar protein sorting 13 homolog D [Source:HGNC Symbol;Acc:HGNC:23595]) HSP 1 Score: 611.298 bits (1575), Expect = 1.348e-174 Identity = 477/1606 (29.70%), Postives = 749/1606 (46.64%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNS-KGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--------------------------QERNVAFCDCRK--------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCG--------------DENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS---CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGM----KCYINASCKNAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V +C IDDC+D DVPL ++ F+ + + S +G LS DYYN GW P IEPWP ++ W K ++ L+ +I+S L+ +T + W ++ Q + + R + FVPF + N GC + + L+ P + ++ G + ++W V T + F+ E AR K+R+ +H H++++++ GW + PV+VDK+G F+R+ D + + + +R+V + +GS + I +RS L + N L P+E + V+ + P +PL + +Q RP + F +PI W V ++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K +R+G M P W + SL+ +RA V Q NRP L+YNIG++++ GRG++I+T MVI RYLLDN+SS L F Q Q I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITL+GA YR+ F+D D LP P++++N + + V F Q+ V+E R + P + + YA DEPTLPP I L ++G S C ++N + LYY+NF+YIA +F + LV DV+P ++V L +K RSQLW + L HEGS PH+P K RS + +LD+ LA + + + ++L K D RR Q W F EG + G+ K ++ A V G +T E P+ + F RPGSG + + V+ DGPTR +++ D + DE +++ +L + T +E+ +EG G+ LIN +PEEL++A ++V + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC + LLP
Sbjct: 2709 ESVCICFIDDCMDCDVPLAELTFSRLNFLQRVRTSPEGYAHFTLSGDYYNRALSGWEPFIEPWPCSVSWQQQAASRLHPPRLKLEAKAKPRLDINITSVLIDQYVSTKESWMADYCKDDKDIESAKSEDWMGSSVDPPCFGQSLPLVYLRTRSTASLTNLEHQIYARAEVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPGVVPEGNGTFLDDTHNVSEWREV--LTGEEIPFEFE--ARGKLRHRH--THDLRIHQLQVRVNGWEQVSPVSVDKVGTFFRYAAPDKNSSSSTIGSPSSRTNIIHPQVYFSSLPPVRVVFAVTMEGSARKVITVRSALIVRNRLETPMELRLDSPSAPDKPVVLPAIMPGDSFAVPLHLTSWRLQARP-----------KGLGVFFCKAPIH-------WTNV------VKTAEISSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALHTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIVCRAEGSLKIFISAPYWLINKTGLPLIFRQDN-AKTDAAGQFEEHELARSLSPLLFCYADKEQPNLCTMRIGRGIHPEGM----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGRYIDTCMVIFAPRYLLDNKSSHKLAFAQREFARGQGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCIWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLRGATYRISFSDTDQLPPPFRIDNFSKVPVVFTQHGVAEPRLRTE--VKPMTSLDYAWDEPTLPPFITLTVKGAGSSEINC-NMNDFQDNRQLYYENFIYIAATYTFSGLQEGTGRPVASNKAITCAELVLDVSPKTQRVILKKKEPGKRSQLWRMTGTGMLAHEGSSVPHNPNKPSAARSTEGSAILDIAGLA---AVTDNRYEPLMLRKPDRRRSTTQTWSFREGKLTCGLHGLVVQAKGGLSGLFDGAEVVLGPDTSMELLGPVPPEQQFINQKMRPGSGMLSIRVIPDGPTRALQITDFCHRKSSRSYEVDELPVTEQELQKLKNPDTEQELEVLVRLEGGIGLSLINKVPEELVFASLTGINVHYTQLATSHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMITAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENLHEKTAEQGGTPIRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHAATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGLGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGPRTQAQRVRKPRCCTGPQGLLP 4241 HSP 2 Score: 295.819 bits (756), Expect = 7.168e-79 Identity = 298/1256 (23.73%), Postives = 552/1256 (43.95%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAA------LRERARNLVKYVQSY----EDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRK---SRDLLQNAVNIP-----APQQSGWWGYL---------WGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDI--TELLNE--VDFEAVNFFT-RDKIFLRGEFSLEGIKLKLL-KDTEIP------ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-------------NPDYK----NDSFRYVVSSREHSKV-----PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEK---GS---SDIRWWVSLKISAPKIILP-NFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPV-------------TNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPE--------INQNYKSVSI-------SFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLS--------ESIGDP-------------------------------IIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKE-----------FDVSIACKAVSILLIKISSNLASENRFA--VSVGRISLSQMNLLM 3274
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+G IG+VTL+IP+ R W I I +++I P EKE E KK LL ++E KW+ +Q+G++ W S AS+ RI+ N+++++++VH+R+ED T PS FA G+ I S +++ E K + KQ+D++ SIY D C ++ ++ ++ + + E ++ P+ ++KRN + PL H PRI +Q+E + + LSQ QY+ + E ER +RQ +++P+V++ ++ +EWW FA+ + I + + RAR+ V Y Y + LL+ D + E +E + + L LRE+ + ++ + L + + P AP+ G G L WG + +PE ++E L + EQ + ++I TE + D +N +T RD +F + L+ + LL K+ P +++ ++ + ES P+ +S + + L LF+RDL NP + + SF +S + S P G +F + +E NP S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ L++ G RW V L ISAP++I P +F+ K P V++DLGR+ ++N D +S +A E E+ +++ TP ++P + S +++ + + V T +Y+ YS+ ++ +VG NW ++ T ++E FN L L+++ + + P ++ N+ L I I++ K+ K C +T + + ++ + +L S + Q+ + V + F ++ + +ES R ++ L + T + +S+ + L+L D G+ D ++ASHK L D P + P +I+ Y+ +S + S ++ + +I ++LD+I N ET+ EL+ F++ F + + Q T F+E +V++ +++LL++ + +A+ ++ ++ I +++N+ M
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSVALLKGAVELENLPLKKDALKEL-ELPFEVKAGFIGKVTLQIPFYRPHVDPWVISISSLHLIGAPEKIQDFNDEKEKLLERERKKALLQALEEKWKNDRQQKGES------YWYSVTASVVTRIVENIELKIQDVHLRFEDGVTNPSHPFAFGICIKNVSMQNAVNEPVQKL-------MRKKQLDVAEFSIYWDVDCTLLGDLPQMELQEAMARSM-ESRSHHYVLEPVFASALLKRNCSKKPLRSRHSPRIDCDIQLETIPLKLSQLQYRQIMEFLKELERKERQVKFRRWKPKVAISKNCREWWYFALNANLYEIREQRKRCTWDFMLHRARDAVSYTDKYFNKLKGGLLSTD---DKEEMCRIEEEQSFEELKILRELVHDRFHKQEELAESLREPQFDSPGACPGAPEPGGGSGMLQYLQSWFPGWGGWYGQQTPE-----------GNVVEGL---SAEQQEQWIPEEILGTEEFFDPTADASCMNTYTKRDHVFAKLNLQLQRGTVTLLHKEQGTPQMNESAFMQLEFSDVKLLAESLPRRNSSLLSVRLGGLFLRDLATEGTMFPLLVFPNPQKEVGRVSQSFGLQTTSADRSDHYPAADPDGPVFEMLYERNPAHS----HFERRLNVSTRPLNIIYNPQAIKKVADFFYKGKVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTLDRLLVGDFIEESKRWTVRLDISAPQVIFPDDFKFKN--PVLVVVDLGRMLLTNTQDNSRRKSRDGSASE----ETQFSDDEYKTPLATPPNTPPPESSSSNGEKTPPFSGVEFSEEQLQAHLMSTKMYERYSLSFMDLQIMVGRVKDNWKHVQDIDVGPTH------VVEKFNVHLQLERRLIYTSDPKYPGAVLSGNLPDLKIHINEDKISALKNCFALLTTPEMKTSDTQIKEKIFPQEEQRGSLQDSVMNLTQSIVLLEQHTREVLVESQLLLAEFKVNCMQLGVESNGRYISVLKVFGTNAHFVKRPYDAEVSLTVHGLLLVDTMQTYGADFDLLMASHKNLSFDIPTGSLRDSRAQSPVSGPNVAHLTDGATLNDRSATSVSLDKILTKEQESLIKLEYQFVSSECPSMNLDSTLQVISLQVNNLDIILNPETIVELIGFLQKSFPKEKDDLSPQPLMTDFERSFREQGTYQSTYEQNTEVAVEIHRLNLLLLR-TVGMANREKYGRKIATASIGGTKVNVSM 1207 HSP 3 Score: 172.94 bits (437), Expect = 1.171e-41 Identity = 147/475 (30.95%), Postives = 236/475 (49.68%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILK-----DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLM--SRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRL-NVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSL--ATDDENRFDQHVAFYIQSKPQSRSISFQ---PVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
CLLD + +DL +MD+++A R K GDL F +R L+E C L L V RNL+ ISHT PD I+G LSSV S+ + +Y+ IRG+L +NLGE + ++ Y Q DP +HT L+G Y M + + +V L L R +G +L R +F + KL YESFS+ +K+I LV + D RY+ + + NVF I P KN+ ++ I Q+ + + S D + F V LN ++ +IFDW+ + +++ T P I T +R + + +S ++ + PV +H E+ +NV + F ++E+ S T+A+IL +YK R +R PFS S+ + SC + ++ LSI++PV+I ++ L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 2151 CLLDCVVVDLQDMDIFAAERHPREYSKAPEDSSGDLIFPSYFVRQTGGSLLTEPCRLKLQVERNLDKEISHTVPDISIHGNLSSVHCSLDLYKYKLIRGLLENNLGEPIEEFMRPYDLQ----DPRIHTVLSGEVYTCMCFLIDMVNVSLELKDPKRKEGAG-----SLARFDFKKCKLLYESFSNQTKSINLVSHSMMAFDTRYAGQKTSPGMTNVFSCIFQPAKNSSTTQGSI---QIELHFRSTKDSSCFTVVLNNLRVFLIFDWLLLVHDFLHT-PSDIKKQNHVTPSRHRNSSSESAIVPKTVKSGVVTKRSSLPVSNERHL-----------EVKVNVTGTEFVVIEDVSCFDTNAIILKGTTVLTYKPRFVDR--PFSGSLFGIEVFSCRLGNEHDTALSIVDPVQIQMELVGN-SSYQNSSGLMDAFNSEDFPPV-LEIQLQALDIRLSYNDVQLFLAIAKSIPEQA 2597 HSP 4 Score: 157.532 bits (397), Expect = 4.282e-37 Identity = 149/534 (27.90%), Postives = 262/534 (49.06%), Query Frame = 2
Query: 3980 KAVYEQVLKSMDNLVY-DDEDEIPK-------------PFIEFEDENMQEDQNPDPSENEIPIVLQSN-----------------ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSS---SNIGQENLQEKS------VSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNS-VDIQNCAAFELKLPKNTV-KSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVK----DLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF 5443
K VYEQVL+++DNLVY +D ++ P P + +++ +N S + + Q + IS+++++L D ++ ++ L Q +EF + + I++A+ + + +L+ K L+ S + S++ Q+ +S V PD+ L S + P K++K + + + + + PK V KS + L I I L+DKK FS+ + ++R +D DFN L I+++ WV++LDFFG+GS D ++ +L K+ P + + +++ +NTKL++ + SL+L T + +N VS + L+ +G++ L GS+ L DL+ G Y +R F S ++ ++ F FKYG PDP L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V AQR +R+ + I AP+LL+P S+ S+++++ +LGK + N+F F
Sbjct: 1539 KHVYEQVLQTLDNLVYSEDLNKYPASATSSPCPDSPLPPLSTCGESSVERKENGLFSHSSLSNTSQKSLSVKEVKSFTQIQATFCISELQVQLSGDLTLGAQG-LVSLKFQDFEVEFSKDHPQTLSIQIALHSLLMEDLLEKNPDSKYKNLMVSRGAPKPSSLAQKEYLSQSCPSVSNVEYPDMPRSLPSHMEEAPNVFQLYQRPTSASRKKQKEVQDKDYPLTPPPSPTVDEPKILVGKSKFDDSLVHINIFLVDKKHPEFSSSYNRVNRSIDVDFNCLDVLITLQTWVVILDFFGIGST-ADNHAMRLPPEGILHNVKLEP--HASMESGLQDPVNTKLDLKVHSLSLVLNKTTSELAKAN---VSKLVAHLEMI--EGDLALQGSIGSLSLSDLTCHGEFYRER-FTTSGEEALI-FQTFKYGRPDPLLRREHDIRVSLRMA-SVQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAIEGQTVRDQAQRCSRVLLDIEAGAPVLLIPESSRSNNLIVANLGKLKVKNKFLF 2060
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Human
Match: VPS13D (vacuolar protein sorting 13 homolog D [Source:HGNC Symbol;Acc:HGNC:23595]) HSP 1 Score: 172.555 bits (436), Expect = 7.659e-42 Identity = 147/475 (30.95%), Postives = 236/475 (49.68%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILK-----DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLM--SRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRL-NVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIV--SLATDDENRFDQHVAFYIQSKPQSRSISFQ---PVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
CLLD + +DL +MD+++A R K GDL F +R L+E C L L V RNL+ ISHT PD I+G LSSV S+ + +Y+ IRG+L +NLGE + ++ Y Q DP +HT L+G Y M + + +V L L R +G +L R +F + KL YESFS+ +K+I LV + D RY+ + + NVF I P KN+ ++ I Q+ + + S D + F V LN ++ +IFDW+ + +++ T P I + T +R + + +S ++ + PV +H E+ +NV + F ++E+ S T+A+IL +YK R +R PFS S+ + SC + ++ LSI++PV+I ++ + +D + E ++I + A IRLS++D+QL I +S Q
Sbjct: 374 CLLDCVVVDLQDMDIFAAERHPREYSKAPEDSSGDLIFPSYFVRQTGGSLLTEPCRLKLQVERNLDKEISHTVPDISIHGNLSSVHCSLDLYKYKLIRGLLENNLGEPIEEFMRPYDLQ----DPRIHTVLSGEVYTCMCFLIDMVNVSLELKDPKRKEGAG-----SLARFDFKKCKLLYESFSNQTKSINLVSHSMMAFDTRYAGQKTSPGMTNVFSCIFQPAKNSSTTQGSI---QIELHFRSTKDSSCFTVVLNNLRVFLIFDWLLLVHDFLHT-PSDIKKQNHVTPSRHRNSSSESAIVPKTVKSGVVTKRSSLPVSNERHL-----------EVKVNVTGTEFVVIEDVSCFDTNAIILKGTTVLTYKPRFVDR--PFSGSLFGIEVFSCRLGNEHDTALSIVDPVQIQMELVGNSSYQNSSGLMDAF--NSEDFPPVLEIQLQALDIRLSYNDVQLFLAIAKSIPEQA 820 HSP 2 Score: 168.703 bits (426), Expect = 1.179e-40 Identity = 158/662 (23.87%), Postives = 269/662 (40.63%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNS-KGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--------------------------QERNVAFCDCRK-------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCG--------------DENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNK 8695
E V +C IDDC+D DVPL ++ F+ + + S +G LS DYYN GW P IEPWP ++ W K ++ L+ +I+S L+ +T + W ++ Q + + R + FVPF + N GC + + L+ P + ++ G + ++W V T + F+ E AR K+R+ +H H++++++ GW + PV+VDK+G F+R+ D + + + +R+V + +GS + I +RS L + N L P+E + V+ + P +PL + +Q RP + F +PI W V ++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K
Sbjct: 932 ESVCICFIDDCMDCDVPLAELTFSRLNFLQRVRTSPEGYAHFTLSGDYYNRALSGWEPFIEPWPCSVSWQQQAASRLHPPRLKLEAKAKPRLDINITSVLIDQYVSTKESWMADYCKDDKDIESAKSEDWMGSSVDPPCFGQSLPLVYLRTRSTASLTNLEHQIYAREVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPGVVPEGNGTFLDDTHNVSEWREV--LTGEEIPFEFE--ARGKLRHRH--THDLRIHQLQVRVNGWEQVSPVSVDKVGTFFRYAAPDKNSSSSTIGSPSSRTNIIHPQVYFSSLPPVRVVFAVTMEGSARKVITVRSALIVRNRLETPMELRLDSPSAPDKPVVLPAIMPGDSFAVPLHLTSWRLQARP-----------KGLGVFFCKAPIH-------WTNV------VKTAEISSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALHTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIVCRAEGSLKIFISAPYWLINKTGLPLIFRQDN-AKTDAAGQFEEHELARSLSPLLFCYADK 1550 HSP 3 Score: 151.369 bits (381), Expect = 2.039e-35 Identity = 101/292 (34.59%), Postives = 166/292 (56.85%), Query Frame = 2
Query: 4586 NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVK----DLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFEN 5449
+ L I I L+DKK FS+ + ++R +D DFN L I+++ WV++LDFFG+GS D ++ +L K+ P + + +++ +NTKL++ + SL+L T + +N VS + L+ +G++ L GS+ L DL+ G Y +R F S ++ ++ F FKYG PDP L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V AQR +R+ + I AP+LL+P S+ S+++++ +LGK + N+F F
Sbjct: 5 DSLVHINIFLVDKKHPEFSSSYNRVNRSIDVDFNCLDVLITLQTWVVILDFFGIGST-ADNHAMRLPPEGILHNVKLEP--HASMESGLQDPVNTKLDLKVHSLSLVLNKTTSELAKAN---VSKLVAHLEMI--EGDLALQGSIGSLSLSDLTCHGEFYRER-FTTSGEEALI-FQTFKYGRPDPLLRREHDIRVSLRMA-SVQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAIEGQTVRDQAQRCSRVLLDIEAGAPVLLIPESSRSNNLIVANLGKLKVKNKFLFAG 285
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Human
Match: VPS13D (vacuolar protein sorting 13 homolog D [Source:HGNC Symbol;Acc:HGNC:23595]) HSP 1 Score: 148.288 bits (373), Expect = 3.710e-37 Identity = 116/384 (30.21%), Postives = 193/384 (50.26%), Query Frame = 2
Query: 9899 AAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSAS 10966
A V G +T E P+ + F RPGSG + + V+ DGPTR +++ D + DE +++ +L + T +E+ +EG G+ LIN +PEEL++A ++V + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK+ S
Sbjct: 6 AEVVLGPDTSMELLGPVPPEQQFINQKMRPGSGMLSIRVIPDGPTRALQITDFCHRKSSRSYEVDELPVTEQELQKLKNPDTEQELEVLVRLEGGIGLSLINKVPEELVFASLTGINVHYTQLATSHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMITAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENLHEKTAEQGGTPIRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKVTQCQS 381
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Celegans
Match: C25H3.11 (pep chromosome:WBcel235:II:5669788:5689030:-1 gene:WBGene00016120.1 transcript:C25H3.11a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C25H3.11) HSP 1 Score: 444.891 bits (1143), Expect = 1.393e-124 Identity = 574/2433 (23.59%), Postives = 1033/2433 (42.46%), Query Frame = 2
Query: 4598 SIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFG-LGSRFYDPKD----VKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTG-----------SIYNQRLF---------------PASNQDRI-----LEFHLFKYGLPDPSLLREEDMRLRAKI--SVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSR------------------CLLDEMKIDLHEMDVYSAIRITDSI--LKDGDL----KFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYKFQAFQTDPI----LHTDLTGI----AYPAMKIELCLNDVQLNL-MSRLQGIDNYANIN-----LCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEID-----NCQLRIKYSSDNDKNS---FIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYI--QSKPQSRSISFQPVIMRK--HYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFS--FSVSQFHLASCVMXXXXXXXXXXXXPVKIII------------KSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTKKFSLTSE------VTLNADISSHLLQIAKNTHKKWSTEFQ------ERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTS--HRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICT-IERLNFPLAP-KMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLI--RLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLD-QLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKH--SVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSF---------ESDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINA------SCKNAAVFGGFETF--KEFYAPLVYKNWF----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQ------------QMDAGYACVMYEENKSESPS----SSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFD-----EGRKIYIGNLKLLTGVISLSVITA--ESLPSDLKQMKKLFP-SLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYE-EKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEG-PSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSS----KEGQPPRRYEPRCLSSSWKLLPYQTSYSNNL 11371
+IK+ +DK F + + ++ + +++ +++ W LL+ G LG ++ + V + SK + SF ++ L R + N++ L +S ++ +L++ M LS S S+ + L G +Y +RL P + R+ + + KY DPS RE D+ + + + Y+HT RF L +F+ F + L +I S V++ A+ + + I C A L+P+++ S +L+ L N FK ++ ++ CLLD + L + + A +I D + +K+ DL F +P S + +LN RNL+ S +PD I +S + + ++ + Y +R V+ N GE +T P L TD+ + Y + + DV LNL + R G A ++ + ++++ +++F D+ ++L+C+++ + D +N N FP+I+ + S N L ++ K+ + L + I+ +DW+ K +I L TD ++ + +++ + Q+ S + I+ + H + +F + + +++S +ILEN ++ A+I A T + + G S + L C M + N I I + K V+ P + L ++ ST ++I V RLS+ D + L +I+ GY + N+ V KP P + Q SK +D IS++ + E + ++DD +PL + +V + + ++ +F +S+DY+N FGW PVIE W I F K T E TLN +++ L+Q + +W+T+ ER+ C R ++ +P+++ N GC + + D +E + +W++V + F L SH ++ +++ GW + PV VD G ++R +++ L R++I + + + + ++S + + N L P+ S + ++PN + +PL + + P+ P PV IE +E SW +V +I EV + + + N+ Y +CT I R +P + +PGH I + AP SL+N LP DV ++ N + S + + + + +V D PLLI+ A + L+ ++ D + LL++ V + +SV + YWIVN++ +PL+ Q + AAG + E+E+A P F+F+++N RVR+G+ N ++ + SEK +L G L+LR ++ +P YN+G+E+R G G++ +T++V+LT+R+LL N+S+ L ++ E + + S T W + ++L V++ D H S F ID SFHV +R + P +RV I L A +++ FT+AD P P ++ENLT++ V YQ S + R H ++ +S + YA D+ + L + Y+I+ GP + L Y+N YI + +SF E + LV + KV LN++ +QLW +L +G I + + +D+ L+LDV +K R+++ + D RR K+QRW + G L C I++ S + AV + K V + W RPGSG + V + GPT ++R+ D+E ++ + A+ ++ + G G+ ++N + EEL+YA + V RR + + IQ+DNQ Q DAG Y++ E P+ + + A L+ YD F R+ + M + L+E +L K +QM S S A +++ +L + + R+ Y G L L G + LSV+T LP DL+ +K+ F L+SF NA + + + + H FET + + +S+ Y +L+ Q + I ++D GNP+G ++D+ + L+ + DV V +G+ +S +K+ SS + + +T D ++E ++R + + S + + +G K L G+ GG ++ T + + G G+ G G+V VTKPV G+ D G+A++ +E+A + + R PR + + LLP +Y+ NL
Sbjct: 925 TIKMTYVDKLHSQFERKYKKRNCSIEIEMDEMEMQMNRRTWTALLEMSGILGVDEHEKSEENVTVTNKTTSSKAVASF----------LVGCTLRARNWRVGMPFPK-----NNTRLGVISLMNTELES-----TMELSESNSNTLAMKLMVDGMRIDDSTPFYSEMYTERLIMLDEQFENASSGFGGPTQTKRRLDDSKRIVIDVVKYLGEDPS--RECDVSIGFHVPSNQKFYYVHTHRFFCALMEFWMQF----NELMNIVTKSKKLKVEEGARAKCSLDVDIQC-AVSFLMPLNSTSPEILLWQADSMKLKNTFKTLSSLKTEIFDKYSIESDYGYDSNIDCLLDYGDMILSNVRAHEARQI-DRLQNMKNLDLVGEKAFQQAAFVLTSPNVFSRRFDLNNKFLRNLDSVFSKNAPDLTIITRISGLKWRMTTQFYVLMRAVIEKNFGEPM-------IPVAETIPTEILQLPTDVMEVECIQKYATLSFRMVFEDVDLNLEVPRKLGEQQKAVLDGKWQPFANVKLERARISFDAFEDNQSELDLICERVELVDT-----TSNGPKNCFPVILQHTDSGKHSRPATPVTSSINSSLMLEAHIMMKKDECPVLTLVLCNARVILAYDWLDDLKQFIM--------LYTDFIPKYSDEIPYHLTESGRRQTFSKTHDGAIIERNAHATDSTSSQFPTFSVKITLRDSDLYILENPFVKNSFAMI---ASTTAVLNMNDNGGYISANLEIQSMKLGWCSMQHEKQTRNLCSNDFSITIALHIDTSLFVNQSKQNKGMVV--TPPIRHALEEQNSTTFLIEIEVVKMICRLSYKDARALKAVIQ----------------GYTK------NYIEV-LKKPLIP-IIVPQPPSKPID-ISMYMIQT-EHTDLWILDDLQSNSIPLLRLSVAHVCLK---KTGERIIASFHVSMDYFNQRIFGWEPVIEEW--KILRFLHNKKDLKQTIEWVAETKSTLNINVTEQLIQQSI----QWNTKLPAILASFERDDFRNQC-TRSSSDHLPYVMKNTTGCEVHFTTAVEDVLTARSE-----QRKSTTRWMTVGRGQEKNFEFPARLL---------LYSHLEREPPRQLIVRVSGWDEISPVNVDSCGTYFRVIKA-----LRPQLKNARLLISVTMENDGKKVVTLKSSIDITNHLPHPIAVQTEGTISEGGEVMSVEPNGVVSVPLEFAHCTLTAYPVGPM---PV------------IEHAE--LSWKSV-----RISGEVCNQTQRLKTRSDSNLNRFYWVCTAIRREYYPEHDFESLPGHSIHLVAPLSLQNLLPIDVEIKIQD-----------NVFAIAASKSMLITSVDITKELSITVSTDRLQSQAPLLIN-KASIGEGTLLFTKMADSKGHLLDMYCHVRLGVAQAISVSLWVPYWIVNKSGIPLIIQQEA-VKWEAAGQMEEHEKAKDRHPLMFSFADENCPKACRVRVGNAYIREN--GYRSILSEKFTLTP-GVQALKLRVE--HKTKPTFYYNLGVEVRPGTGRYKDTQVVLLTSRFLLQNQSTVALSVCHHDLVEMEKDHVHLAAQSSTT--WNENYAGRRMLCVRRADVKHW-----SCPFLIDRIGSFHVTMRDADET-PRFIRVEIILTSAVFQITFTNADLYPPPIRIENLTDVPV-LYQQESSTPARPHLRTICKSRSVVDYAWDDLYGNKKMVLQVFENRSKSYEISLPGPAEPLVYENSSYIQLAHSFTKRSGKGSGEFTDEQELVLETMQK-GKVMLNKQCRTEDNQLW------KLCADGCIENVGMNHR--KRSDSRLVLDV---------LEKTGGRLMMMERDKRRNKWQRWTWMPDGRL-------CLIDSPHLMMTSKQTEAVVVEADGLAPKGEDGVAVTQIWKVQRQRPGSGTLGVEFLHSGPTVLIRITDREFQTRAVSEPMIQAANKLAVLDVSITMR-GGLGISVVNGLQEELIYAHFGGIVVSARRVDKTYQMTGSVDVIQIDNQLLSSDRWQVLYCQPDAGQN---YDDQMLELPAGIPVTGRPALKLEMNCTSMKHYD--SFDCFRLKVCDMSVQLDELLLWKIVQMAQS-SDAASSVQQRALNLPPNTELERHDPLRTRRWYFGTLDLEMGHVGLSVVTVSKSGLPRDLRLLKQQFNVKLISFENASVALPPFRQYHYFETSSFLLESLQKFYLAELQKQTLSIIVTLDAFGNPLGLVTDLKDSFQGLFIEGDVQRFVAGLGYGLSNSVSKVASSMASGVGALTFDQDHELKRRHNMIRSHSSSSTPLTHLYSGVKGLGVGVLGGATAMFTNVASESRKSGLVKGMVWGVATGVVDTVTKPVQGVFDFVEGTASAMKELAMPATGVRRATALCRVRIPRLCRNLYHLLP---AYNPNL 3165 HSP 2 Score: 182.185 bits (461), Expect = 5.920e-45 Identity = 117/388 (30.15%), Postives = 205/388 (52.84%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEF--QNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGK-IWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDC---IVNPISLHGIMKRNINPLPMNHQ-PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR------ERARNLVKYVQSYEDYLLNKDSNLEA 1147
M+ ++ ++L+ IG+Y+E+ +T + L G + LEN+PLK T L L + P+ VKSGL+G++TL +P T RS+ W + + D+ II GP S+ K ++ E Q E K+ +L+ +E++ + + E + W + ASL +L N+Q+ + NVH+RYED++T+P Q F G+ I + ++++ W+ + K+++L G SIY ++K + ENI+ Q KL P++ I+ P S M++N + P+ + PR ++ + + + L++ Q A++ E+ RF+R + H K+RP ++ E+ KEWW FA + A R ERA Y ++Y L+ +N A
Sbjct: 1 MLENVVAWVLNNYIGEYLEDLNTDQLSVALLSGQVELENVPLKKTALRKL-DLPVEVKSGLLGKLTLSVPITHIRSEPWTLKLSDVLIIVGPLSSEKRYDVEAVEQVEQQRKEQMLEELELRHKTALFDLCGIPIPESQDSW--WGASLISTVLNNIQLILNNVHIRYEDDTTIPGQTFNCGIRIQKMTMQTTNHHWKPGFAEPQKGTNTFKKLELGGFSIYWNSKTTM-----TENIEDSKQLKLILAPETSSNAYIIQPCSAELRMEKNSSKFPLKSKVPRFKFFMRPDILGLELTRHQMTQLRAVNREWARFERARQHRKWRPLCTIGENAKEWWKFAYNRVLEESRRANANRTWDFAHERASRFNAYCRAYRKRLIGLIANPNA 380
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Celegans
Match: C25H3.11 (pep chromosome:WBcel235:II:5669788:5689030:-1 gene:WBGene00016120.1 transcript:C25H3.11b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C25H3.11) HSP 1 Score: 441.425 bits (1134), Expect = 1.574e-123 Identity = 575/2433 (23.63%), Postives = 1032/2433 (42.42%), Query Frame = 2
Query: 4598 SIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFG-LGSRFYDPKD----VKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTG-----------SIYNQRLF---------------PASNQDRI-----LEFHLFKYGLPDPSLLREEDMRLRAKI--SVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSR------------------CLLDEMKIDLHEMDVYSAIRITDSI--LKDGDL----KFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYKFQAFQTDPI----LHTDLTGI----AYPAMKIELCLNDVQLNL-MSRLQGIDNYANIN-----LCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEID-----NCQLRIKYSSDNDKNS---FIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYI--QSKPQSRSISFQPVIMRK--HYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFS--FSVSQFHLASCVMXXXXXXXXXXXXPVKIII------------KSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTKKFSLTSE------VTLNADISSHLLQIAKNTHKKWSTEFQ------ERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTS--HRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICT-IERLNFPLAP-KMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLI--RLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLD-QLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKH--SVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSF---------ESDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINA------SCKNAAVFGGFETF--KEFYAPLVYKNWF----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQ------------QMDAGYACVMYEENKSESPS----SSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFD-----EGRKIYIGNLKLLTGVISLSVITA--ESLPSDLKQMKKLFP-SLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYE-EKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEG-PSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSS----KEGQPPRRYEPRCLSSSWKLLPYQTSYSNNL 11371
+IK+ +DK F + + ++ + +++ +++ W LL+ G LG ++ + V + SK + SF ++ L R + N++ L +S ++ +L++ M LS S S+ + L G +Y +RL P + R+ + + KY DPS RE D+ + + + Y+HT RF L +F+ F + L +I S V++ A+ + + I C A L+P+++ S +L+ L N FK ++ ++ CLLD + L + + A +I D + +K+ DL F +P S + +LN RNL+ S +PD I +S + + ++ + Y +R V+ N GE +T P L TD+ + Y + + DV LNL + R G A ++ + ++++ +++F D+ ++L+C+++ + D +N N FP+I+ + S N L ++ K+ + L + I+ +DW+ K +I L TD ++ + +++ + Q+ S + I+ + H + +F + + +++S +ILEN ++ A+I A T + + G S + L C M + N I I + K V+ P + L ++ ST ++I V RLS+ D + L +I+ GY + N+ V KP P + Q SK +D IS++ + E + ++DD +PL + +V + + ++ +F +S+DY+N FGW PVIE W I F K T E TLN +++ L+Q + +W+T+ ER+ C R ++ +P+++ N GC + + D +E + +W++V + F L SH ++ +++ GW + PV VD G ++R +++ L R++I + + + + ++S + + N L P+ SV +PN + +PL + + P+ P PV IE +E SW +V +I EV + + + N+ Y +CT I R +P + +PGH I + AP SL+N LP DV ++ N + S + + + + +V D PLLI+ A + L+ ++ D + LL++ V + +SV + YWIVN++ +PL+ Q + AAG + E+E+A P F+F+++N RVR+G+ N ++ + SEK +L G L+LR ++ +P YN+G+E+R G G++ +T++V+LT+R+LL N+S+ L ++ E + + S T W + ++L V++ D H S F ID SFHV +R + P +RV I L A +++ FT+AD P P ++ENLT++ V YQ S + R H ++ +S + YA D+ + L + Y+I+ GP + L Y+N YI + +SF E + LV + KV LN++ +QLW +L +G I + + +D+ L+LDV +K R+++ + D RR K+QRW + G L C I++ S + AV + K V + W RPGSG + V + GPT ++R+ D+E ++ + A+ ++ + G G+ ++N + EEL+YA + V RR + + IQ+DNQ Q DAG Y++ E P+ + + A L+ YD F R+ + M + L+E +L K +QM S S A +++ +L + + R+ Y G L L G + LSV+T LP DL+ +K+ F L+SF NA + + + + H FET + + +S+ Y +L+ Q + I ++D GNP+G ++D+ + L+ + DV V +G+ +S +K+ SS + + +T D ++E ++R + + S + + +G K L G+ GG ++ T + + G G+ G G+V VTKPV G+ D G+A++ +E+A + + R PR + + LLP +Y+ NL
Sbjct: 925 TIKMTYVDKLHSQFERKYKKRNCSIEIEMDEMEMQMNRRTWTALLEMSGILGVDEHEKSEENVTVTNKTTSSKAVASF----------LVGCTLRARNWRVGMPFPK-----NNTRLGVISLMNTELES-----TMELSESNSNTLAMKLMVDGMRIDDSTPFYSEMYTERLIMLDEQFENASSGFGGPTQTKRRLDDSKRIVIDVVKYLGEDPS--RECDVSIGFHVPSNQKFYYVHTHRFFCALMEFWMQF----NELMNIVTKSKKLKVEEGARAKCSLDVDIQC-AVSFLMPLNSTSPEILLWQADSMKLKNTFKTLSSLKTEIFDKYSIESDYGYDSNIDCLLDYGDMILSNVRAHEARQI-DRLQNMKNLDLVGEKAFQQAAFVLTSPNVFSRRFDLNNKFLRNLDSVFSKNAPDLTIITRISGLKWRMTTQFYVLMRAVIEKNFGEPM-------IPVAETIPTEILQLPTDVMEVECIQKYATLSFRMVFEDVDLNLEVPRKLGEQQKAVLDGKWQPFANVKLERARISFDAFEDNQSELDLICERVELVDT-----TSNGPKNCFPVILQHTDSGKHSRPATPVTSSINSSLMLEAHIMMKKDECPVLTLVLCNARVILAYDWLDDLKQFIM--------LYTDFIPKYSDEIPYHLTESGRRQTFSKTHDGAIIERNAHATDSTSSQFPTFSVKITLRDSDLYILENPFVKNSFAMI---ASTTAVLNMNDNGGYISANLEIQSMKLGWCSMQHEKQTRNLCSNDFSITIALHIDTSLFVNQSKQNKGMVV--TPPIRHALEEQNSTTFLIEIEVVKMICRLSYKDARALKAVIQ----------------GYTK------NYIEV-LKKPLIP-IIVPQPPSKPID-ISMYMIQT-EHTDLWILDDLQSNSIPLLRLSVAHVCLK---KTGERIIASFHVSMDYFNQRIFGWEPVIEEW--KILRFLHNKKDLKQTIEWVAETKSTLNINVTEQLIQQSI----QWNTKLPAILASFERDDFRNQC-TRSSSDHLPYVMKNTTGCEVHFTTAVEDVLTARSE-----QRKSTTRWMTVGRGQEKNFEFPARLL---------LYSHLEREPPRQLIVRVSGWDEISPVNVDSCGTYFRVIKA-----LRPQLKNARLLISVTMENDGKKVVTLKSSIDITNHLPHPIAVQTEGTKGGEVMSV--EPNGVVSVPLEFAHCTLTAYPVGPM---PV------------IEHAE--LSWKSV-----RISGEVCNQTQRLKTRSDSNLNRFYWVCTAIRREYYPEHDFESLPGHSIHLVAPLSLQNLLPIDVEIKIQD-----------NVFAIAASKSMLITSVDITKELSITVSTDRLQSQAPLLIN-KASIGEGTLLFTKMADSKGHLLDMYCHVRLGVAQAISVSLWVPYWIVNKSGIPLIIQQEA-VKWEAAGQMEEHEKAKDRHPLMFSFADENCPKACRVRVGNAYIREN--GYRSILSEKFTLTP-GVQALKLRVE--HKTKPTFYYNLGVEVRPGTGRYKDTQVVLLTSRFLLQNQSTVALSVCHHDLVEMEKDHVHLAAQSSTT--WNENYAGRRMLCVRRADVKHW-----SCPFLIDRIGSFHVTMRDADET-PRFIRVEIILTSAVFQITFTNADLYPPPIRIENLTDVPV-LYQQESSTPARPHLRTICKSRSVVDYAWDDLYGNKKMVLQVFENRSKSYEISLPGPAEPLVYENSSYIQLAHSFTKRSGKGSGEFTDEQELVLETMQK-GKVMLNKQCRTEDNQLW------KLCADGCIENVGMNHR--KRSDSRLVLDV---------LEKTGGRLMMMERDKRRNKWQRWTWMPDGRL-------CLIDSPHLMMTSKQTEAVVVEADGLAPKGEDGVAVTQIWKVQRQRPGSGTLGVEFLHSGPTVLIRITDREFQTRAVSEPMIQAANKLAVLDVSITMR-GGLGISVVNGLQEELIYAHFGGIVVSARRVDKTYQMTGSVDVIQIDNQLLSSDRWQVLYCQPDAGQN---YDDQMLELPAGIPVTGRPALKLEMNCTSMKHYD--SFDCFRLKVCDMSVQLDELLLWKIVQMAQS-SDAASSVQQRALNLPPNTELERHDPLRTRRWYFGTLDLEMGHVGLSVVTVSKSGLPRDLRLLKQQFNVKLISFENASVALPPFRQYHYFETSSFLLESLQKFYLAELQKQTLSIIVTLDAFGNPLGLVTDLKDSFQGLFIEGDVQRFVAGLGYGLSNSVSKVASSMASGVGALTFDQDHELKRRHNMIRSHSSSSTPLTHLYSGVKGLGVGVLGGATAMFTNVASESRKSGLVKGMVWGVATGVVDTVTKPVQGVFDFVEGTASAMKELAMPATGVRRATALCRVRIPRLCRNLYHLLP---AYNPNL 3163 HSP 2 Score: 182.185 bits (461), Expect = 5.869e-45 Identity = 117/388 (30.15%), Postives = 205/388 (52.84%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEF--QNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGK-IWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDC---IVNPISLHGIMKRNINPLPMNHQ-PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR------ERARNLVKYVQSYEDYLLNKDSNLEA 1147
M+ ++ ++L+ IG+Y+E+ +T + L G + LEN+PLK T L L + P+ VKSGL+G++TL +P T RS+ W + + D+ II GP S+ K ++ E Q E K+ +L+ +E++ + + E + W + ASL +L N+Q+ + NVH+RYED++T+P Q F G+ I + ++++ W+ + K+++L G SIY ++K + ENI+ Q KL P++ I+ P S M++N + P+ + PR ++ + + + L++ Q A++ E+ RF+R + H K+RP ++ E+ KEWW FA + A R ERA Y ++Y L+ +N A
Sbjct: 1 MLENVVAWVLNNYIGEYLEDLNTDQLSVALLSGQVELENVPLKKTALRKL-DLPVEVKSGLLGKLTLSVPITHIRSEPWTLKLSDVLIIVGPLSSEKRYDVEAVEQVEQQRKEQMLEELELRHKTALFDLCGIPIPESQDSW--WGASLISTVLNNIQLILNNVHIRYEDDTTIPGQTFNCGIRIQKMTMQTTNHHWKPGFAEPQKGTNTFKKLELGGFSIYWNSKTTM-----TENIEDSKQLKLILAPETSSNAYIIQPCSAELRMEKNSSKFPLKSKVPRFKFFMRPDILGLELTRHQMTQLRAVNREWARFERARQHRKWRPLCTIGENAKEWWKFAYNRVLEESRRANANRTWDFAHERASRFNAYCRAYRKRLIGLIANPNA 380
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Celegans
Match: C25H3.11 (pep chromosome:WBcel235:II:5669788:5689030:-1 gene:WBGene00016120.1 transcript:C25H3.11c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C25H3.11) HSP 1 Score: 438.728 bits (1127), Expect = 1.154e-122 Identity = 568/2421 (23.46%), Postives = 1027/2421 (42.42%), Query Frame = 2
Query: 4598 SIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFG-LGSRFYDPKD----VKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTG-----------SIYNQRLF---------------PASNQDRI-----LEFHLFKYGLPDPSLLREEDMRLRAKI--SVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSR------------------CLLDEMKIDLHEMDVYSAIRITDSI--LKDGDL----KFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYKFQAFQTDPI----LHTDLTGI----AYPAMKIELCLNDVQLNL-MSRLQGIDNYANIN-----LCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEID-----NCQLRIKYSSDNDKNS---FIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYI--QSKPQSRSISFQPVIMRK--HYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFS--FSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTKKFSLTSE------VTLNADISSHLLQIAKNTHKKWSTEFQ------ERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTS--HRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICT-IERLNFPLAP-KMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLI--RLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLD-QLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKH--SVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSF---------ESDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINA------SCKNAAVFGGFETF--KEFYAPLVYKNWF----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQ------------QMDAGYACVMYEENKSESPS----SSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFD-----EGRKIYIGNLKLLTGVISLSVITA--ESLPSDLKQMKKLFP-SLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYE-EKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEG-PSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSS----KEGQPPRRYEPRCLSSSWKLLPYQTSYSNNL 11371
+IK+ +DK F + + ++ + +++ +++ W LL+ G LG ++ + V + SK + SF ++ L R + N++ L +S ++ +L++ M LS S S+ + L G +Y +RL P + R+ + + KY DPS RE D+ + + + Y+HT RF L +F+ F + L +I S V++ A+ + + I C A L+P+++ S +L+ L N FK ++ ++ CLLD + L + + A +I D + +K+ DL F +P S + +LN RNL+ S +PD I +S + + ++ + Y +R V+ N GE +T P L TD+ + Y + + DV LNL + R G A ++ + ++++ +++F D+ ++L+C+++ + D +N N FP+I+ + S N L ++ K+ + L + I+ +DW+ K +I L TD ++ + +++ + Q+ S + I+ + H + +F + + +++S +ILEN ++ A+I A T + + G S + L C M + N I I + + + + + ++I V RLS+ D + L +I+ GY + N+ V KP P + Q SK +D IS++ + E + ++DD +PL + +V + + ++ +F +S+DY+N FGW PVIE W I F K T E TLN +++ L+Q + +W+T+ ER+ C R ++ +P+++ N GC + + D +E + +W++V + F L SH ++ +++ GW + PV VD G ++R +++ L R++I + + + + ++S + + N L P+ S + ++PN + +PL + + P+ P PV IE +E SW +V +I EV + + + N+ Y +CT I R +P + +PGH I + AP SL+N LP DV ++ N + S + + + + +V D PLLI+ A + L+ ++ D + LL++ V + +SV + YWIVN++ +PL+ Q + AAG + E+E+A P F+F+++N RVR+G+ N ++ + SEK +L G L+LR ++ +P YN+G+E+R G G++ +T++V+LT+R+LL N+S+ L ++ E + + S T W + ++L V++ D H S F ID SFHV +R + P +RV I L A +++ FT+AD P P ++ENLT++ V YQ S + R H ++ +S + YA D+ + L + Y+I+ GP + L Y+N YI + +SF E + LV + KV LN++ +QLW +L +G I + + +D+ L+LDV +K R+++ + D RR K+QRW + G L C I++ S + AV + K V + W RPGSG + V + GPT ++R+ D+E ++ + A+ ++ + G G+ ++N + EEL+YA + V RR + + IQ+DNQ Q DAG Y++ E P+ + + A L+ YD F R+ + M + L+E +L K +QM S S A +++ +L + + R+ Y G L L G + LSV+T LP DL+ +K+ F L+SF NA + + + + H FET + + +S+ Y +L+ Q + I ++D GNP+G ++D+ + L+ + DV V +G+ +S +K+ SS + + +T D ++E ++R + + S + + +G K L G+ GG ++ T + + G G+ G G+V VTKPV G+ D G+A++ +E+A + + R PR + + LLP +Y+ NL
Sbjct: 925 TIKMTYVDKLHSQFERKYKKRNCSIEIEMDEMEMQMNRRTWTALLEMSGILGVDEHEKSEENVTVTNKTTSSKAVASF----------LVGCTLRARNWRVGMPFPK-----NNTRLGVISLMNTELES-----TMELSESNSNTLAMKLMVDGMRIDDSTPFYSEMYTERLIMLDEQFENASSGFGGPTQTKRRLDDSKRIVIDVVKYLGEDPS--RECDVSIGFHVPSNQKFYYVHTHRFFCALMEFWMQF----NELMNIVTKSKKLKVEEGARAKCSLDVDIQC-AVSFLMPLNSTSPEILLWQADSMKLKNTFKTLSSLKTEIFDKYSIESDYGYDSNIDCLLDYGDMILSNVRAHEARQI-DRLQNMKNLDLVGEKAFQQAAFVLTSPNVFSRRFDLNNKFLRNLDSVFSKNAPDLTIITRISGLKWRMTTQFYVLMRAVIEKNFGEPM-------IPVAETIPTEILQLPTDVMEVECIQKYATLSFRMVFEDVDLNLEVPRKLGEQQKAVLDGKWQPFANVKLERARISFDAFEDNQSELDLICERVELVDT-----TSNGPKNCFPVILQHTDSGKHSRPATPVTSSINSSLMLEAHIMMKKDECPVLTLVLCNARVILAYDWLDDLKQFIM--------LYTDFIPKYSDEIPYHLTESGRRQTFSKTHDGAIIERNAHATDSTSSQFPTFSVKITLRDSDLYILENPFVKNSFAMI---ASTTAVLNMNDNGGYISANLEIQSMKLGWCSMQHEKQTRNLCSNDFSITIALHIDTSLFVNQSKQNKGMVVTPPIRHALEIEVVKMICRLSYKDARALKAVIQ----------------GYTK------NYIEV-LKKPLIP-IIVPQPPSKPID-ISMYMIQT-EHTDLWILDDLQSNSIPLLRLSVAHVCLK---KTGERIIASFHVSMDYFNQRIFGWEPVIEEW--KILRFLHNKKDLKQTIEWVAETKSTLNINVTEQLIQQSI----QWNTKLPAILASFERDDFRNQC-TRSSSDHLPYVMKNTTGCEVHFTTAVEDVLTARSE-----QRKSTTRWMTVGRGQEKNFEFPARLL---------LYSHLEREPPRQLIVRVSGWDEISPVNVDSCGTYFRVIKA-----LRPQLKNARLLISVTMENDGKKVVTLKSSIDITNHLPHPIAVQTEGTISEGGEVMSVEPNGVVSVPLEFAHCTLTAYPVGPM---PV------------IEHAE--LSWKSV-----RISGEVCNQTQRLKTRSDSNLNRFYWVCTAIRREYYPEHDFESLPGHSIHLVAPLSLQNLLPIDVEIKIQD-----------NVFAIAASKSMLITSVDITKELSITVSTDRLQSQAPLLIN-KASIGEGTLLFTKMADSKGHLLDMYCHVRLGVAQAISVSLWVPYWIVNKSGIPLIIQQEA-VKWEAAGQMEEHEKAKDRHPLMFSFADENCPKACRVRVGNAYIREN--GYRSILSEKFTLTP-GVQALKLRVE--HKTKPTFYYNLGVEVRPGTGRYKDTQVVLLTSRFLLQNQSTVALSVCHHDLVEMEKDHVHLAAQSSTT--WNENYAGRRMLCVRRADVKHW-----SCPFLIDRIGSFHVTMRDADET-PRFIRVEIILTSAVFQITFTNADLYPPPIRIENLTDVPV-LYQQESSTPARPHLRTICKSRSVVDYAWDDLYGNKKMVLQVFENRSKSYEISLPGPAEPLVYENSSYIQLAHSFTKRSGKGSGEFTDEQELVLETMQK-GKVMLNKQCRTEDNQLW------KLCADGCIENVGMNHR--KRSDSRLVLDV---------LEKTGGRLMMMERDKRRNKWQRWTWMPDGRL-------CLIDSPHLMMTSKQTEAVVVEADGLAPKGEDGVAVTQIWKVQRQRPGSGTLGVEFLHSGPTVLIRITDREFQTRAVSEPMIQAANKLAVLDVSITMR-GGLGISVVNGLQEELIYAHFGGIVVSARRVDKTYQMTGSVDVIQIDNQLLSSDRWQVLYCQPDAGQN---YDDQMLELPAGIPVTGRPALKLEMNCTSMKHYD--SFDCFRLKVCDMSVQLDELLLWKIVQMAQS-SDAASSVQQRALNLPPNTELERHDPLRTRRWYFGTLDLEMGHVGLSVVTVSKSGLPRDLRLLKQQFNVKLISFENASVALPPFRQYHYFETSSFLLESLQKFYLAELQKQTLSIIVTLDAFGNPLGLVTDLKDSFQGLFIEGDVQRFVAGLGYGLSNSVSKVASSMASGVGALTFDQDHELKRRHNMIRSHSSSSTPLTHLYSGVKGLGVGVLGGATAMFTNVASESRKSGLVKGMVWGVATGVVDTVTKPVQGVFDFVEGTASAMKELAMPATGVRRATALCRVRIPRLCRNLYHLLP---AYNPNL 3155 HSP 2 Score: 182.185 bits (461), Expect = 5.864e-45 Identity = 117/388 (30.15%), Postives = 205/388 (52.84%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEF--QNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGK-IWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDC---IVNPISLHGIMKRNINPLPMNHQ-PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR------ERARNLVKYVQSYEDYLLNKDSNLEA 1147
M+ ++ ++L+ IG+Y+E+ +T + L G + LEN+PLK T L L + P+ VKSGL+G++TL +P T RS+ W + + D+ II GP S+ K ++ E Q E K+ +L+ +E++ + + E + W + ASL +L N+Q+ + NVH+RYED++T+P Q F G+ I + ++++ W+ + K+++L G SIY ++K + ENI+ Q KL P++ I+ P S M++N + P+ + PR ++ + + + L++ Q A++ E+ RF+R + H K+RP ++ E+ KEWW FA + A R ERA Y ++Y L+ +N A
Sbjct: 1 MLENVVAWVLNNYIGEYLEDLNTDQLSVALLSGQVELENVPLKKTALRKL-DLPVEVKSGLLGKLTLSVPITHIRSEPWTLKLSDVLIIVGPLSSEKRYDVEAVEQVEQQRKEQMLEELELRHKTALFDLCGIPIPESQDSW--WGASLISTVLNNIQLILNNVHIRYEDDTTIPGQTFNCGIRIQKMTMQTTNHHWKPGFAEPQKGTNTFKKLELGGFSIYWNSKTTM-----TENIEDSKQLKLILAPETSSNAYIIQPCSAELRMEKNSSKFPLKSKVPRFKFFMRPDILGLELTRHQMTQLRAVNREWARFERARQHRKWRPLCTIGENAKEWWKFAYNRVLEESRRANANRTWDFAHERASRFNAYCRAYRKRLIGLIANPNA 380
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Celegans
Match: C25H3.11 (pep chromosome:WBcel235:II:5669788:5689030:-1 gene:WBGene00016120.1 transcript:C25H3.11d.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C25H3.11) HSP 1 Score: 435.261 bits (1118), Expect = 1.240e-121 Identity = 569/2421 (23.50%), Postives = 1026/2421 (42.38%), Query Frame = 2
Query: 4598 SIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFG-LGSRFYDPKD----VKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTG-----------SIYNQRLF---------------PASNQDRI-----LEFHLFKYGLPDPSLLREEDMRLRAKI--SVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSR------------------CLLDEMKIDLHEMDVYSAIRITDSI--LKDGDL----KFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYKFQAFQTDPI----LHTDLTGI----AYPAMKIELCLNDVQLNL-MSRLQGIDNYANIN-----LCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEID-----NCQLRIKYSSDNDKNS---FIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYI--QSKPQSRSISFQPVIMRK--HYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFS--FSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTKKFSLTSE------VTLNADISSHLLQIAKNTHKKWSTEFQ------ERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTS--HRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICT-IERLNFPLAP-KMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLI--RLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLD-QLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKH--SVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSF---------ESDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINA------SCKNAAVFGGFETF--KEFYAPLVYKNWF----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQ------------QMDAGYACVMYEENKSESPS----SSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFD-----EGRKIYIGNLKLLTGVISLSVITA--ESLPSDLKQMKKLFP-SLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYE-EKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEG-PSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSS----KEGQPPRRYEPRCLSSSWKLLPYQTSYSNNL 11371
+IK+ +DK F + + ++ + +++ +++ W LL+ G LG ++ + V + SK + SF ++ L R + N++ L +S ++ +L++ M LS S S+ + L G +Y +RL P + R+ + + KY DPS RE D+ + + + Y+HT RF L +F+ F + L +I S V++ A+ + + I C A L+P+++ S +L+ L N FK ++ ++ CLLD + L + + A +I D + +K+ DL F +P S + +LN RNL+ S +PD I +S + + ++ + Y +R V+ N GE +T P L TD+ + Y + + DV LNL + R G A ++ + ++++ +++F D+ ++L+C+++ + D +N N FP+I+ + S N L ++ K+ + L + I+ +DW+ K +I L TD ++ + +++ + Q+ S + I+ + H + +F + + +++S +ILEN ++ A+I A T + + G S + L C M + N I I + + + + + ++I V RLS+ D + L +I+ GY + N+ V KP P + Q SK +D IS++ + E + ++DD +PL + +V + + ++ +F +S+DY+N FGW PVIE W I F K T E TLN +++ L+Q + +W+T+ ER+ C R ++ +P+++ N GC + + D +E + +W++V + F L SH ++ +++ GW + PV VD G ++R +++ L R++I + + + + ++S + + N L P+ SV +PN + +PL + + P+ P PV IE +E SW +V +I EV + + + N+ Y +CT I R +P + +PGH I + AP SL+N LP DV ++ N + S + + + + +V D PLLI+ A + L+ ++ D + LL++ V + +SV + YWIVN++ +PL+ Q + AAG + E+E+A P F+F+++N RVR+G+ N ++ + SEK +L G L+LR ++ +P YN+G+E+R G G++ +T++V+LT+R+LL N+S+ L ++ E + + S T W + ++L V++ D H S F ID SFHV +R + P +RV I L A +++ FT+AD P P ++ENLT++ V YQ S + R H ++ +S + YA D+ + L + Y+I+ GP + L Y+N YI + +SF E + LV + KV LN++ +QLW +L +G I + + +D+ L+LDV +K R+++ + D RR K+QRW + G L C I++ S + AV + K V + W RPGSG + V + GPT ++R+ D+E ++ + A+ ++ + G G+ ++N + EEL+YA + V RR + + IQ+DNQ Q DAG Y++ E P+ + + A L+ YD F R+ + M + L+E +L K +QM S S A +++ +L + + R+ Y G L L G + LSV+T LP DL+ +K+ F L+SF NA + + + + H FET + + +S+ Y +L+ Q + I ++D GNP+G ++D+ + L+ + DV V +G+ +S +K+ SS + + +T D ++E ++R + + S + + +G K L G+ GG ++ T + + G G+ G G+V VTKPV G+ D G+A++ +E+A + + R PR + + LLP +Y+ NL
Sbjct: 925 TIKMTYVDKLHSQFERKYKKRNCSIEIEMDEMEMQMNRRTWTALLEMSGILGVDEHEKSEENVTVTNKTTSSKAVASF----------LVGCTLRARNWRVGMPFPK-----NNTRLGVISLMNTELES-----TMELSESNSNTLAMKLMVDGMRIDDSTPFYSEMYTERLIMLDEQFENASSGFGGPTQTKRRLDDSKRIVIDVVKYLGEDPS--RECDVSIGFHVPSNQKFYYVHTHRFFCALMEFWMQF----NELMNIVTKSKKLKVEEGARAKCSLDVDIQC-AVSFLMPLNSTSPEILLWQADSMKLKNTFKTLSSLKTEIFDKYSIESDYGYDSNIDCLLDYGDMILSNVRAHEARQI-DRLQNMKNLDLVGEKAFQQAAFVLTSPNVFSRRFDLNNKFLRNLDSVFSKNAPDLTIITRISGLKWRMTTQFYVLMRAVIEKNFGEPM-------IPVAETIPTEILQLPTDVMEVECIQKYATLSFRMVFEDVDLNLEVPRKLGEQQKAVLDGKWQPFANVKLERARISFDAFEDNQSELDLICERVELVDT-----TSNGPKNCFPVILQHTDSGKHSRPATPVTSSINSSLMLEAHIMMKKDECPVLTLVLCNARVILAYDWLDDLKQFIM--------LYTDFIPKYSDEIPYHLTESGRRQTFSKTHDGAIIERNAHATDSTSSQFPTFSVKITLRDSDLYILENPFVKNSFAMI---ASTTAVLNMNDNGGYISANLEIQSMKLGWCSMQHEKQTRNLCSNDFSITIALHIDTSLFVNQSKQNKGMVVTPPIRHALEIEVVKMICRLSYKDARALKAVIQ----------------GYTK------NYIEV-LKKPLIP-IIVPQPPSKPID-ISMYMIQT-EHTDLWILDDLQSNSIPLLRLSVAHVCLK---KTGERIIASFHVSMDYFNQRIFGWEPVIEEW--KILRFLHNKKDLKQTIEWVAETKSTLNINVTEQLIQQSI----QWNTKLPAILASFERDDFRNQC-TRSSSDHLPYVMKNTTGCEVHFTTAVEDVLTARSE-----QRKSTTRWMTVGRGQEKNFEFPARLL---------LYSHLEREPPRQLIVRVSGWDEISPVNVDSCGTYFRVIKA-----LRPQLKNARLLISVTMENDGKKVVTLKSSIDITNHLPHPIAVQTEGTKGGEVMSV--EPNGVVSVPLEFAHCTLTAYPVGPM---PV------------IEHAE--LSWKSV-----RISGEVCNQTQRLKTRSDSNLNRFYWVCTAIRREYYPEHDFESLPGHSIHLVAPLSLQNLLPIDVEIKIQD-----------NVFAIAASKSMLITSVDITKELSITVSTDRLQSQAPLLIN-KASIGEGTLLFTKMADSKGHLLDMYCHVRLGVAQAISVSLWVPYWIVNKSGIPLIIQQEA-VKWEAAGQMEEHEKAKDRHPLMFSFADENCPKACRVRVGNAYIREN--GYRSILSEKFTLTP-GVQALKLRVE--HKTKPTFYYNLGVEVRPGTGRYKDTQVVLLTSRFLLQNQSTVALSVCHHDLVEMEKDHVHLAAQSSTT--WNENYAGRRMLCVRRADVKHW-----SCPFLIDRIGSFHVTMRDADET-PRFIRVEIILTSAVFQITFTNADLYPPPIRIENLTDVPV-LYQQESSTPARPHLRTICKSRSVVDYAWDDLYGNKKMVLQVFENRSKSYEISLPGPAEPLVYENSSYIQLAHSFTKRSGKGSGEFTDEQELVLETMQK-GKVMLNKQCRTEDNQLW------KLCADGCIENVGMNHR--KRSDSRLVLDV---------LEKTGGRLMMMERDKRRNKWQRWTWMPDGRL-------CLIDSPHLMMTSKQTEAVVVEADGLAPKGEDGVAVTQIWKVQRQRPGSGTLGVEFLHSGPTVLIRITDREFQTRAVSEPMIQAANKLAVLDVSITMR-GGLGISVVNGLQEELIYAHFGGIVVSARRVDKTYQMTGSVDVIQIDNQLLSSDRWQVLYCQPDAGQN---YDDQMLELPAGIPVTGRPALKLEMNCTSMKHYD--SFDCFRLKVCDMSVQLDELLLWKIVQMAQS-SDAASSVQQRALNLPPNTELERHDPLRTRRWYFGTLDLEMGHVGLSVVTVSKSGLPRDLRLLKQQFNVKLISFENASVALPPFRQYHYFETSSFLLESLQKFYLAELQKQTLSIIVTLDAFGNPLGLVTDLKDSFQGLFIEGDVQRFVAGLGYGLSNSVSKVASSMASGVGALTFDQDHELKRRHNMIRSHSSSSTPLTHLYSGVKGLGVGVLGGATAMFTNVASESRKSGLVKGMVWGVATGVVDTVTKPVQGVFDFVEGTASAMKELAMPATGVRRATALCRVRIPRLCRNLYHLLP---AYNPNL 3153 HSP 2 Score: 182.185 bits (461), Expect = 6.114e-45 Identity = 117/388 (30.15%), Postives = 205/388 (52.84%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEF--QNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGK-IWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDC---IVNPISLHGIMKRNINPLPMNHQ-PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR------ERARNLVKYVQSYEDYLLNKDSNLEA 1147
M+ ++ ++L+ IG+Y+E+ +T + L G + LEN+PLK T L L + P+ VKSGL+G++TL +P T RS+ W + + D+ II GP S+ K ++ E Q E K+ +L+ +E++ + + E + W + ASL +L N+Q+ + NVH+RYED++T+P Q F G+ I + ++++ W+ + K+++L G SIY ++K + ENI+ Q KL P++ I+ P S M++N + P+ + PR ++ + + + L++ Q A++ E+ RF+R + H K+RP ++ E+ KEWW FA + A R ERA Y ++Y L+ +N A
Sbjct: 1 MLENVVAWVLNNYIGEYLEDLNTDQLSVALLSGQVELENVPLKKTALRKL-DLPVEVKSGLLGKLTLSVPITHIRSEPWTLKLSDVLIIVGPLSSEKRYDVEAVEQVEQQRKEQMLEELELRHKTALFDLCGIPIPESQDSW--WGASLISTVLNNIQLILNNVHIRYEDDTTIPGQTFNCGIRIQKMTMQTTNHHWKPGFAEPQKGTNTFKKLELGGFSIYWNSKTTM-----TENIEDSKQLKLILAPETSSNAYIIQPCSAELRMEKNSSKFPLKSKVPRFKFFMRPDILGLELTRHQMTQLRAVNREWARFERARQHRKWRPLCTIGENAKEWWKFAYNRVLEESRRANANRTWDFAHERASRFNAYCRAYRKRLIGLIANPNA 380
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Celegans
Match: T08G11.1 (pep chromosome:WBcel235:I:8905644:8916805:-1 gene:WBGene00011629.1 transcript:T08G11.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T08G11.1) HSP 1 Score: 140.584 bits (353), Expect = 2.080e-32 Identity = 162/737 (21.98%), Postives = 316/737 (42.88%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEF-QNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEV-SVENIKSVLQEKL----NEMPKSDCIVNPISLHGIMKRNINPLPMNHQ---PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESP-KEWWVFAITEHTRHIFKGAALRERARN-----LVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAME------HIGRKSRDLL--QNAVNIPAPQQSGW--WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDF-EAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYF----SRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRS 2140
++ L+ LL+R +G +V+N + + G++ GD++L+NL +K T L+D F+ PI +K G + + L+IP+ ++ ++ + +I PN E++ +N +K+ L +E + RK+ R+ D ++ + +I++NLQI V N+HVR+ED T + FA G+ ++ + K++D W +T + ++ K V L ++IY ++ + +S++ E I+ LQE + N + I+ PI + +K N P P+I L + M + I++ + QY+ + + ERF+ ++KYRP ++ + K WW FA T +R R N + K+ Q Y +A R E+ D ++E + ++ R+ +L + + + +GW WG W +P+ + SQ E + P + +L +D+ E + K F+ +F + ++ ++ D + + L+ E RP S+ + ++ L + + ++ R+ P + +TNPL N +++ ++ P+ I + I N + F S + A A KAR++ + +E S R + ++I +I + + E +L D+G L V + +D S
Sbjct: 1 MVFESLVADLLNRFLGDFVDNLDSSQLNIGIWGGDVKLDNLQVKETALDD-FDLPIKLKYGYLSSLVLKIPWKNLYNEPVIATVDGLNLIVVPNKGVVYNEEKVAKNIQEIKQKTLARLE-EARKERRKPKDPQAD------TFTEKMITQIIKNLQISVSNIHVRFEDRYTNRHRPFAMGITLEKLNFKTTDENWR-ETIHKDVVKIIYKLVSLQNLAIYWNSSTEFISDLDDKEAIRKKLQETIHNGKNGPEGYNYILEPIQMEAKLKLNQKPETDGSNWKIPKIDLAVDMHALSIAIGKFQYQDVLLFLEAQERFNAAGQYLKYRPNLNEFKGHYKAWWKFAYTSILEE-----KVRRRRNNWSWDRMQKHRQLVRKY-------QKAWVRRQTEASPGADVQATIKEAEKKLDVFNVNVARQQAELEIDREGLTRQEDKPTGWVAWGKSWFGGGGGPTPD--KKKGAKDIGSQFQEAMTPEEK-----------AKLFEAIDYQENIPPTNYPKEFVENKFDFKLGQVAIVVDGAVSM---QLLKLVASVEQRPSASAMHVESSIQELRMDGCGTE--------IIRVRD----PSIPWMSFLLDTNPLKG----NYDQLVKLAVAPINIKYQAPAINNAIDVFKPPESVRLNQLTALAMSRYEEVKARSATGLAYAVEHRS---RLVLDVQIQPARIYVSEGGTYSSEKPTLLADMGLLSVVTVDNSSVDTS 681 HSP 2 Score: 75.8702 bits (185), Expect = 8.285e-13 Identity = 68/232 (29.31%), Postives = 110/232 (47.41%), Query Frame = 2
Query: 10712 YDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSD--QDVYNMVKHFAHGIG---------------DSTAKICSSASRVISEITMDPEYEEKRQR-LFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPYQT 11353
++R VF + + I HY Q Q + +D +GNP G + D++ G++ L+ Q + FA G+ + +I + + ++ +T D +Y +KRQ L + G A+ G K L G+ GGI V T+ +G K EG G G KG++GVVT+PV G++D A+G+ S R +A +++E P R PR L + PY +
Sbjct: 2867 FERKCVFYSPEQLNSEIISHYAKQFIKQVYVLVLGLDIIGNPFGLVRDLSAGVEDLFYQPFQGAIQGPEEFAAGVALGVQSMFGHAVGGAAGAVGRITGTVGKGVAALTFDDDYMKKRQEDLNRKPQSFGEGMAR---GLKGLGMGVVGGITGVVTKPIEGAKQEGGFGFVKGVGKGLIGVVTRPVSGVVDFASGTMNSVRAVAGTNREAGPLR--PPRVLREDKIVKPYSS 3093
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Fly
Match: Vps13D (gene:FBgn0052113 transcript:FBtr0273390) HSP 1 Score: 805.053 bits (2078), Expect = 0.000e+0 Identity = 1006/4094 (24.57%), Postives = 1778/4094 (43.43%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS--NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEG-KIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLD-----TKCKIMSEVSV-ENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNI--NPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEA---EFRNNLESDLELDCLLQLREVAMEHIGRKS------RDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIPI-------LEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSRE-HSKVPGGEIFALAFETNPLPSTCPPNIKRI---LAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIR----WWVSLKISAPKII-LPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTN------------IYDEYSIKLENVMALVGNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLE----LTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQ------------PLSES----------------------IGDPIIEFT------------YKISQKSGSSDIQ-RQINLIFSDLDVIANQETLTELLSFMR--LLFSNDSLQIF-DQKPETPN----------------------------------TTEFKEFDVSIACKAVSILLIKISSNLASENRFAVSVGRISLSQMN--LLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSR-LKHNIAAVARTAILSSEESTRIGFLNSVKLHLRMLSPILALPRHK-------------------TSNQIILVNLGDIEITSDTQSLQVAFSDAIVISKE------LPIENGGYDIGIC-TDNNAKFPLVNKIDLMLK----FSKLHDPPKIL----IECEXXXXXXXXXXKAVYEQVLKSM------DNLVYDDEDEIPKPFIEFEDENMQEDQNPDPSENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEKSVSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNSVDIQNCAAFELKLPKNTVKSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIV-YIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF--ENNDRSRC--------LLDEMKIDLHEMDVYSAIRITDSILKDGD---LKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHL-KSYKFQAFQTDPILHT-------DLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEID---NCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFT-APYGIVSLATDDENRFDQHVAFYIQSKPQ-SRSISFQPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWS---SLNFD--------NVNTSKPSQPNLTALQKLSKFVDRISIH---HLDDYEFVSVCLIDDCIDADVPLFDIKFN----NVTVDIELGNSK---------GLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFW----LTTKK---FSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDEN-----KWISV-PDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSL--NIPLEAIXXXXXXXXXXXV-VLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNK--VYLICTIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLI----RLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNC---NEDQNKP--IMALPASF-LTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQ---LRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLP--PKSCIPYALDEPTLPPIINLCIEGGTQSCYDIN-KVGPGQNLYYDNFLYIAIRNSFE-----SDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKN-----------AAVFGGFETFKEFYAPL-----VYKNWFRPGSGEVLVSVVTDGPTRVMRLID---KEDESILSQSQLDFNA--------DSSTFS-------NIEMHFSVEGFGVFLINHIP-EELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSK---EAELLEEDFSSLQHQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKK-LFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFE-NARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSS-WKLLPYQTSYSN 11365
M+ LIT++L+ +GKY+EN ++ + L G++ LEN+P++ L F P+ V +G I ++ L+IP +FR+ W I IE ++ I P + NW +EKE + K +LD+ E WR + +Q ++ WL Y ++ I+ N+++++ +VH R+ED TG+ I + + +S D +W + + ++ K V+L +S+Y D KC+ S + E + S E+ + I+ PI KR+ + +PR+S L + +V I +S+ Q + E + + + RP +V +P WW +A H K + ++Y+ Y+ +LN + NL A EF+ +ESD ++ L +R + E + K + +N + P GW+ SP + Q + S + LE DI L E + + D +F G FS++ +K ++ TE + +E+ N + PQ +S+ + I L+ +++ D + Y++ + ++ P + A + + L N + L I S+ L +++ I + ++ + S + + RN A+ ++ +K E S + W ++I AP+II L N+ K VLLD G+L++ + R I V SD + E ++TPCS+P S ++ S + + +T+ N IYD+Y I N+ LV + E K +S L++ FN L L+++ + + P+ ++ +LI +++ + + K +++ N YR ++ R+ NL + + V I FV+ +I+++S R +AEL + + L++ T IS+ + L+L DA + G + ++ASH+ + D P S +GD EF KI + S+ +Q ++ F+ LD+IANQ+T+ E+L+F + LL N IF + PE+P T + +F+V V++ KI+++ S+ S+G I + + H ++ +R+ + ++L L I + + ETK S+ D I+ + F + Y H P FL V CI+ YF R L+ ++ A ++E F+ V+ + + P+ + TS +++ NLG I ++ + F+++ I + L I+ G Y + N P+++ + L+ +S +D K L I+ +L +S ++ YE +L+S+ N V ++ DE+ ++ +++ N D +E+ I +Q ++ ++ L + + ++ L + ++ + + + KD+E+ + + L + L S + ++ S ++N + S SCPDL +S N +S++ PS FY + + K F + E K + +I K G S + + + FN L I +E W + DFFGL S + +V++ P + + I+++ +KL + ++ F+ L ++ N++ + Y+K + G L + DL+ G+IY Q+ + + L F ++K L D L D +I++ V YIHT+RF EL+ F ++ LQ Q + + + + R +++++ I +P+++LP S + ++I LG+FSL N F F +NN S+ +LD M+IDL ++++S R + D + + + +N + +E C L+L + RNL+ + PD + G S + ++++QY+ IR L +N+GE TD + +Y + + L T +++ I + I + L DV L L L + A L ++F +S L + FSD S+ I+L+ I + D R S +N+ NVF I+ P K +R E+ + +C+ + +S + + LN M+ + ++ K+++ +P +V+ A + I KPQ SIS TE +N+ +S E S+L ++A+IL YK N P S ++ + SC + ++ + LSII+P +II+ C+ ++L K IRLS+ D++L ++ R QT P N+ + + + ++ F+ +N + + + Q+ ++ + D +SV +IDDC+DADVPL ++ + N T + N K G + +SV+YYN GW PV E W + W + L KK ++S+ L +++S +++ K W+ +F + R + F+PF + N G P+ ++ P+Y + + + + W SV P+ T FD + + K+R+ + SH H+I ++I GW+ P++VDK+G+F+R + D+ T RIV +I GS Q I ++S L + N L N+ L+ S SS+ V+KPN + +PL + + + H+ S + D F + E ++ C + Q + A + NK +Y I R + + +PGH IT+ P + N L CD+ F + + S + ++ Q S+ LD+F L I +SH +I +L D Q R L + V + S P + + IS+ WI+N+T LPL+Y Q G + T AAG E+E A P F+FS++ S +RLG +NM W + S K LRA + + Y IG+ +R GRG + T V L+ R+ L NRS + L F+Q C N D+ P I++ P FHWP D +Q++ V+ + SK G I S ++ +R N + LR+ I + A + ++FTDA LP P +++N + + + N S+ + + P P+S + Y +D+P I+ + GG + IN K + L Y NF+YIA + +FE + LV V KKV + +K+S RSQLW +SN +L HEGS P Q D +A L+LD++ AP P + +++ + +R Q W F G ++ C+ N ++ AV G E + + RPGSG++ +S DGP + + D K++ L+ L +A D S N+E+ V+G G+ +I P EE+++ ++++ + + +++L + I IQ+DNQ +DA ++ + S +QNA LK + + +F ++ + + P LEEK++LK FL K + ++ F + + + ++ Y NL + + LS T+ LP +L + KK L +L+ F +A I +D Y FET ++ + + HY NQ++ A I GSVDFLGNP+GF +D++ G+ L + V ++VK+ HGI +STAK+ + S + ++ +D E RQR+ E + SG + A AG K L +G+ GG+ S+ G +G G G KG+VG VTKP+ G+LDLA+ +A++ RE +R S P R+ PRC++ + LLP YSN
Sbjct: 1 MLRDLITWVLNTYLGKYLENLNSAQLSVALLSGEVELENIPIRKDALRS-FNLPVEVTAGSIRKIKLQIPVRQFRTSPWCISIEGLFCIICPKNLDNWD-YEKEKLQDLEYKLAVLDTAEAGWRSEKGKQMESYYFSSYNNWLKYGTNMATNIIDNIELKISDVHFRFEDIVDTGKSKICTGIKIGSLTAQSCDCDWTNGSYKMNNNEMNYKLVELKELSVYWDLLHEDIKCQSYSNQEILEKMHSTC-----ELRSHNFIIKPICATARWKRDKCQQVIRTKDKPRVSCELLVPEVVIDISKVQRLQMLDKLSEIRQVKEVRQYRLKRPTCTVESNPIAWWKYATICHGFDFKKNEEKWLMLKENLRYMLLYKSIILNPNENLSAADKEFKAYIESDRKISDLTIMRRICFEKVFTKGFAFESQNEQGKNMLFHWFPNWMGWYA---------NSPSTPNNEQ-----------------DESLKHLEDDILVAL-ENSLQNSSDLKSDAVF--GHFSIKLLKGLVILQTEDKLNDGRNKSMEMQFNNFSAYLQLSPQLTSYTVGISLQEVYLIDKTS--SDTMHNYLIKPQTGNTATPNQLVKNAALQEDILFQLQYENCNHLRFQLNIKSKGLDLIYNEDAIQWLLDFLADSN-----SFKYSPRNRVAKKTDFMKNWNEMFSGNEVNRKIWMFEIEIFAPRIIFLENY--KVSNSLMVLLDFGKLEMRKME---VKRVIPVIESAVTENTSD-DDETYLTPCSTPPASEKSGSESPTLLENPKTESFLNKNVQLEYVLHNKIYDKYLINFTNLQVLVCKYEERWQACLKTSSN---FHLIDKFNINLTLEQRNIFTVDPEYPSFMLFGTCPTILIHGNEELINNCCNIMKPIIKASKEMENIYRGGNT----IYASERIKNLAE-----DDRSRVVIEFVMDQLVIEMQSTERSIAELQIIGARAGLTKEPHETNISMSVHGLLLVDAIQSFGPDFELLVASHRHVGMDSLSGSLKHSAIVSPTSPGSPNFFDRATSPHMITKAVQNIKMGDRSTEFCDDEDSTALISVDIKIVPPNESNSMQLHTTSITFNSLDIIANQDTIIEILNFAKRTLLAQN----IFPSESPESPKEATEAPVDVVDQVDHKSHNEIFFDFFRLNILLLYTIKRDKFNVGRKVGTVTLTEAKINASFQSDLSIIGSLGGIQVIDITSEAFCHPRILSVGRDQILRASDTNKQTVLSQLSNEIYS-NNYNEETK---SDESDAISFQSHWSDKTTCTF-QMRMASASYTHCPRFLRDVNACIT----YFKRSLREFATSIGNKATDMAKE-----FVQQVRA-VEQIGPVYPQRNRQDNWLDIIISSPIIILPISNTSTNVLIANLGKISCSNAVKCDSDEFNESYTIEIKNTYVYSLNIDEGEYSFNVHPAKNKDAIPILHDTAITLQLYAGYSDDNDEDKQLNRFSIKGSMVEALKVSLNRKQYELLLESIRYATNFSNEVLNESDELD------QNGDIEPTANID-AESIISTTIQFSVPVFQINLQ----NEYHNDLINLTFKNFNVKHI-SKGFDKDVEVVLKSV-LMEDLKSDLTSPFRNMVTSVDLEQKIKKN-EMTSSSCPDLPSYC-NSLKNRSSSV---PSCFYNHMQVK-------------VFGGDQKYTSSNKNELGKKKESQTLVIYKSHTGRSAQNGKLEQTSSIQFNCLNLAICVERWYTIFDFFGLVS----------VDNVNEKYPEEMKLVEKHIDKVC-SKLKVSIRSFNFTLIRNESLLSRVNVSNAVFMILQ-DPYSK----IVEGCLGSVSVNDLTKYGNIYKQKFLTSGTE--ALNF-VYKKKLVDLEALNTLDTDSTLRINMSAVHYIHTKRFSTELHVFVKDLLQLQTPV----IRKLKKHGSEQNMRPSKMKLVIQADSPVIVLPSSYNRNEVIIAYLGQFSLKNSFHFASDNNIISKMSATPSKDEILDVMRIDLVNINLFSGERSSMKAKADQEKDRIIIADMNFLRLGQPFFNESCFLHLQLERNLSADAHRVCPDISVQGTFSKLSGIINIQQYKLIRSFLNNNIGEQTDDIYMNYHNNSCTSIERLSTINLMPKNEVSKIVSILISIRILLEDVSLLLA--LNTSQSAAIEPLACIHFLKSTLEIDLFSDGSQDIDLISSNILIVDERNESDKSNE--NVFKNILEPSKKEVRIENSVQVEVHCRKKATFSK------YTIMLNNMRVFALLSFLDQLKSYLQEDSPAPVVNNAANQ-----------IAQKPQIDTSIS--------------------TEYVVNITDSEIIFAEECSRLDSNAIILKSTTVICYKPNSNIV-PLSLDINHLEIFSCTLDAEEESALSIIDPFTLIIELRSN-CL--------NILIQKH------------LNIRLSYVDVKLFSRMARLLPTQTSRPKNVISKADSDLEKAAPLVAMGFEISDCLYAMQINNWRINDAAIWLSQQKQNTYRNPALEMKTAVVDASLISVFIIDDCMDADVPLLEVSLSKFLLNFTFQTQDPNPKETNIRHYSLGNIDTEVSVNYYNRRLSGWEPVAETWESNLNWKYTKGHLDNKKRFEIGISSKQMLKLNVTSTFIELFHMVLKNWTNDFNDNGAK----NFRQRSPFIPFALQNLSGTPLLFK-------PIYAPLGDLTRSDLQQVELIKNWYSVQPNETKT---FDF--SQKSKLRH--VHSHQLNLHQIFVQIHGWTLIGPISVDKVGMFFRTTKLDSQ-----FLTKSRIVFDISLIGSAQKLIKVKSSLGVINKLDRNVFLKMTLKGTHSDGLSSISVIKPNDELSVPLKFIDASLYV-----AHNTSESDAYEDTGFSN----EEILWKACGKDDTRQLL--------------AGYDTNKSILYTFVNISREIYHCKEQNLPGHKITLLPPLKINNMLCCDLMFKIHEHATGR----------INSSESVNIYNVNICQPLNLSITLDNFQLSGQLKI----PVSHRGVIEPKLKLIDIQKRELHVRVSIQSVPGKGMELYISAPVWIINKTGLPLIYKQEGTSHT-AAGQFEEHETARQVAPLMFSFSDQEGSPALVLRLGKAYGSNNM------WCKSFSTHK-DLADRDLRAENTKGS-----YAIGISVRRGRGLYACTTFVTLSPRFHLHNRSGYKLEFMQL-CDIVNYDRPDPRKIISAPVDCNFAFHWPNWDQEQIICVRIPEIECCCWSK---GIPIKDVQSLYINVR----NEWGEMFFLRLEIISKDATFILLFTDARTLPPPIRIDNCSEVVI----NFSQLRSKPVWITPVRPQSSLSYVMDDPLGQQILLMEAPGGNMIEFPINNKNNIKKTLTYTNFIYIAFQGTFERSNEEENTHRQLVLGV--RGKKVVIVEKNSGDRSQLWLMNSNGQLEHEGSTPP---IQTNDANAV-RLVLDLE-KAPNP----MEFTNLVVRTPNKQRVTTQTWRFENGRLM-------CHANMCVQSRFGESGLKPNYEAVVGRTENRASSSKQIPIGQHIVAQKLRPGSGQLELSTKMDGPISTIEICDIKIKQNSVFLTPDLLWMHASLNNRQITDKGKVSFVHEYLINVEL---VKGIGISIIARKPCEEIMFISLDHINCDIVQSALENSLDLNIAYIQIDNQLLDAVSPIALHTQT-SNDLEETQNAVVLKLKMLPSPNKNAIIFKYLTLDLKPSTASLEEKLILKVAS-FLGYGKINRQNLSVQYQFENTDDKPFLQDMKRYYFENLSIGATQVRLSAFTSSKLPVELHETKKALGLTLIKFEDALIELDRYSDKLHFETMDVYRKELKKHYINQVKWHAAAILGSVDFLGNPLGFANDLSEGVSGLIFEGSVKSLVKNVTHGISNSTAKLTETLSDSLGKVVLDDHDNETRQRILELQSNTSGGHLA---AGLKGLGFGLLGGVTSIVRHTYDGATSDGVPGFLSGLGKGLVGTVTKPIIGVLDLASETASAVRETSRDSHRNAPERKRLPRCVTGAPGGLLPL---YSN 3783
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Fly
Match: Vps13 (gene:FBgn0033194 transcript:FBtr0310024) HSP 1 Score: 179.874 bits (455), Expect = 3.488e-44 Identity = 204/878 (23.23%), Postives = 393/878 (44.76%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLN-------EMPKSDCIVNPISLHGIMKRNINPL---PMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRP-RVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDT-----------EIP-ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIK---RILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVGNWNE-LESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELT 2545
++ ++ +L++V+G Y+EN +K G++ GD+ L+NL ++ L++L + P+ + G +G++ L+IP+ SQ + IED+Y++ PN+N + EKE + E +KK LD++E +K++ D K + + L +I+ NLQ+++ NVH+RYED +T S F+ G+ + ++D +WE K + + + K +LS +S YL+ ++ + N KS L ++ P + ++ PIS + +K N+NP P +P+I L L+ME++ + L+ Q+ + L D R + KYRP + ++WW FAIT R + ++ Y Q Y++ L+K S + E LE++L++ LL +R+ I ++ + + + ++ +T+ + L + + TS+ EK+ R E D+ E +EA+ R F L +++ L KD E+P ++ +N RP + I G+R + V L +ND +V S+ + E+F FETNPL C +K R L I+ + TI+ LI Q + + AA I N K R++ ++ +++K + + +++PN + H+ G N S ++ + +P ES+ L++L A + + + N YD +++ +++V LV E ++ L++ S ++ ++ P + + CV++ + PNI ++ ++ +L+++S+ ++ L
Sbjct: 1 MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIRENALDEL-DLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEKEAKYEMDLKKAALDALEAARKKELEM--DQPKADA----GFAEKLTAQIVNNLQVQITNVHLRYEDTTTTGSP-FSFGISLHELELYTTDCDWE-KCYMAQQASQVFKIANLSCLSAYLNCGGQLYA-----NNKSDLSQQFKTNIACKETKPNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKIDLTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAITSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETELDVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGW----GGGAKKDDQTTSQKLVEKFEAAMTSEEKEKMY-RAIGYQENAKPTDLPE-----SYEAI----------RMNFKLIALEVGLYKDERNSSAATKDFHELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLT---RNDYTPLLVESKITDEFNLLEVF---FETNPLDKLCDQRVKVVARPLQITYDAPTIL---ALINAFQTPGDVTLSKFEDAASTKISNFKERSATGMQYMIDKKAV---------LDVDILLMPNILVVPHK--------GVYDAGNVSLLVVSMGQVHLSSQPRR-ESN---------------KLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPWQNALAEANST--EMHVLRPVSLKV-TAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFLA 799 HSP 2 Score: 97.8265 bits (242), Expect = 2.797e-19 Identity = 98/399 (24.56%), Postives = 168/399 (42.11%), Query Frame = 2
Query: 10235 LLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLK-------FQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMF---LSPSKEAELLEEDFSSLQ------HQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFPSLM-----SFTNAQIII---DVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ-----------------DVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSS---KEGQPPR 11299
L V I +IQVDNQ +D + V+ P S + SLK QR + + V F + R+ I ++ L +MF +S A+ +D S++ + H E +K + NL L I +S A SD K + SL+ + T+ ++ ++R + F + + I HY Q Q + +D LGNP G + + G++ L+ + V ++ H G + +KI + + ++ +T D +Y++KR++ +N + + K L G G+ V T+ G +D G G F G KG +G+V +P G++D A+GS + + A +S K +PPR
Sbjct: 2783 LHVKINRIQVDNQFLDPIFPVVLA---PIPPPKSVASTTSLKPFIECSMVQRIMPNS-TVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVESIELPLSAFFEEHSLEEQKSFYDNLHLGPLKIHVSFSMA---GSDTKALPGFLGSLVQGVGVTLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDEDYQKKRRQGIQNK--PKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGARDNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPR 3172 HSP 3 Score: 64.3142 bits (155), Expect = 3.316e-9 Identity = 88/364 (24.18%), Postives = 159/364 (43.68%), Query Frame = 2
Query: 8471 YDDQNRL-LELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTI-TLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNLLVF 9556
YD + ++ ++L VK ++R G + + + S +W++N+T + L Y +S T H PE S P F F +K F + + ++ N + WSEK+ L+ G+ + A+ N Y +G+ L + TK + Y++ N+ F + + + D P + L + + WPR+D L V ++D P + F +C L + ++ + V + T +G Y + FTD +P L N T Q+ ++ E + +L KS I YA D+PT P ++ + G + D+ + G G+ + D + + SF L +L+F
Sbjct: 2328 YDSEMKVDMDLYVKTENR-HGSLMLTLFSPFWMINKTGMMLTY-KSETTSVEVLYHPPE-----YSGPILFTFRDK----LFFDKKKASIRIDNGQ-----WSEKIPLDVAGSVGEVICFAN------NQKYPVGVHNHLTQNSL--TKQITFIPFYIVCNKCHFDIELQEQSRPAD---PWLHLEPNEMEPLWPRNDTKNNLVV-RVDGKITP----AFDFTEVICT-----LLKLEDSKYGGINVDVQTTEGGVY-ITFTDYKPADAPGLLINHTGKQIVYH----EKGTKNEHILNAKSTIMYAWDDPTGPKML---VFGTNKEETDLKRDGIGEVIMQDGGKVLWV--SFLDGLQRVLLF 2644
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Fly
Match: Vps13 (gene:FBgn0033194 transcript:FBtr0088910) HSP 1 Score: 179.874 bits (455), Expect = 3.488e-44 Identity = 204/878 (23.23%), Postives = 393/878 (44.76%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLN-------EMPKSDCIVNPISLHGIMKRNINPL---PMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRP-RVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDT-----------EIP-ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIK---RILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVGNWNE-LESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELT 2545
++ ++ +L++V+G Y+EN +K G++ GD+ L+NL ++ L++L + P+ + G +G++ L+IP+ SQ + IED+Y++ PN+N + EKE + E +KK LD++E +K++ D K + + L +I+ NLQ+++ NVH+RYED +T S F+ G+ + ++D +WE K + + + K +LS +S YL+ ++ + N KS L ++ P + ++ PIS + +K N+NP P +P+I L L+ME++ + L+ Q+ + L D R + KYRP + ++WW FAIT R + ++ Y Q Y++ L+K S + E LE++L++ LL +R+ I ++ + + + ++ +T+ + L + + TS+ EK+ R E D+ E +EA+ R F L +++ L KD E+P ++ +N RP + I G+R + V L +ND +V S+ + E+F FETNPL C +K R L I+ + TI+ LI Q + + AA I N K R++ ++ +++K + + +++PN + H+ G N S ++ + +P ES+ L++L A + + + N YD +++ +++V LV E ++ L++ S ++ ++ P + + CV++ + PNI ++ ++ +L+++S+ ++ L
Sbjct: 1 MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIRENALDEL-DLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEKEAKYEMDLKKAALDALEAARKKELEM--DQPKADA----GFAEKLTAQIVNNLQVQITNVHLRYEDTTTTGSP-FSFGISLHELELYTTDCDWE-KCYMAQQASQVFKIANLSCLSAYLNCGGQLYA-----NNKSDLSQQFKTNIACKETKPNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKIDLTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAITSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETELDVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGW----GGGAKKDDQTTSQKLVEKFEAAMTSEEKEKMY-RAIGYQENAKPTDLPE-----SYEAI----------RMNFKLIALEVGLYKDERNSSAATKDFHELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLT---RNDYTPLLVESKITDEFNLLEVF---FETNPLDKLCDQRVKVVARPLQITYDAPTIL---ALINAFQTPGDVTLSKFEDAASTKISNFKERSATGMQYMIDKKAV---------LDVDILLMPNILVVPHK--------GVYDAGNVSLLVVSMGQVHLSSQPRR-ESN---------------KLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPWQNALAEANST--EMHVLRPVSLKV-TAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFLA 799 HSP 2 Score: 97.8265 bits (242), Expect = 2.797e-19 Identity = 98/399 (24.56%), Postives = 168/399 (42.11%), Query Frame = 2
Query: 10235 LLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLK-------FQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMF---LSPSKEAELLEEDFSSLQ------HQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFPSLM-----SFTNAQIII---DVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ-----------------DVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSS---KEGQPPR 11299
L V I +IQVDNQ +D + V+ P S + SLK QR + + V F + R+ I ++ L +MF +S A+ +D S++ + H E +K + NL L I +S A SD K + SL+ + T+ ++ ++R + F + + I HY Q Q + +D LGNP G + + G++ L+ + V ++ H G + +KI + + ++ +T D +Y++KR++ +N + + K L G G+ V T+ G +D G G F G KG +G+V +P G++D A+GS + + A +S K +PPR
Sbjct: 2783 LHVKINRIQVDNQFLDPIFPVVLA---PIPPPKSVASTTSLKPFIECSMVQRIMPNS-TVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVESIELPLSAFFEEHSLEEQKSFYDNLHLGPLKIHVSFSMA---GSDTKALPGFLGSLVQGVGVTLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDEDYQKKRRQGIQNK--PKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGARDNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPR 3172 HSP 3 Score: 64.3142 bits (155), Expect = 3.316e-9 Identity = 88/364 (24.18%), Postives = 159/364 (43.68%), Query Frame = 2
Query: 8471 YDDQNRL-LELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTI-TLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNLLVF 9556
YD + ++ ++L VK ++R G + + + S +W++N+T + L Y +S T H PE S P F F +K F + + ++ N + WSEK+ L+ G+ + A+ N Y +G+ L + TK + Y++ N+ F + + + D P + L + + WPR+D L V ++D P + F +C L + ++ + V + T +G Y + FTD +P L N T Q+ ++ E + +L KS I YA D+PT P ++ + G + D+ + G G+ + D + + SF L +L+F
Sbjct: 2328 YDSEMKVDMDLYVKTENR-HGSLMLTLFSPFWMINKTGMMLTY-KSETTSVEVLYHPPE-----YSGPILFTFRDK----LFFDKKKASIRIDNGQ-----WSEKIPLDVAGSVGEVICFAN------NQKYPVGVHNHLTQNSL--TKQITFIPFYIVCNKCHFDIELQEQSRPAD---PWLHLEPNEMEPLWPRNDTKNNLVV-RVDGKITP----AFDFTEVICT-----LLKLEDSKYGGINVDVQTTEGGVY-ITFTDYKPADAPGLLINHTGKQIVYH----EKGTKNEHILNAKSTIMYAWDDPTGPKML---VFGTNKEETDLKRDGIGEVIMQDGGKVLWV--SFLDGLQRVLLF 2644
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Fly
Match: Vps13B (gene:FBgn0039727 transcript:FBtr0085505) HSP 1 Score: 59.3066 bits (142), Expect = 1.197e-7 Identity = 45/194 (23.20%), Postives = 79/194 (40.72%), Query Frame = 2
Query: 10712 YDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYS-----------------DQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEY---EEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDL 11233
Y+R + QS+ HY + A + GS++ LG+P G +TG++ S Q +++++ G +S K+ S +R + +T+D E+ E R+R AR G + G L + G + + + G+ G KGIVG +TKP+ G ++
Sbjct: 3353 YERQQILTVPLRFGQSLGLHYLSGAIFGAGWVVGSLEILGSPSGLARSFSTGLRDFVSMPVQGLFRGPWGFLVGVTQGSASLLRNVTAGTVNSVTKLAGSVARNLDRLTLDAEHIELTEARRR----ARPQGFADG-LTQGLTGLGISLLGAVGGLAHH---TLEARSSVGVIAGLTKGIVGALTKPISGAAEM 3538 HSP 2 Score: 54.6842 bits (130), Expect = 2.525e-6 Identity = 53/220 (24.09%), Postives = 109/220 (49.55%), Query Frame = 2
Query: 38 ITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAG-PNSNWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWE-SKTELTTELDVLNKQVDLSGISIYLDTK 691
IT +L + KYV+NF ++ + L++G++ +NL L+ VLE+ P+ + SG I +++ +P+T+ S+ I+I I +A P+S K FQ E K +++ +Q D K G S V + +I+ N+ ++ N+ ++Y ++ + S + + + S+ +W+ + ++ V+ K + +S ++I LD +
Sbjct: 8 ITPILLSYVAKYVKNFRDEDAQVSLWEGEVTFQNLDLRLEVLEEELNLPVELVSGHIHELSILVPWTKLMSEPVKIVINTIEFVAKLPDSESKQRRASFQREQRRKS----------KRESVEQPDQTKSPGPASSSSVVN---KIINNINLQCHNIILKYVEDDIVVS------MNVQTLNFSSAGEDWKPTMVDIHPVSVVMRKLLQVSDLTICLDKR 208
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Fly
Match: Vps13B (gene:FBgn0039727 transcript:FBtr0300825) HSP 1 Score: 53.9138 bits (128), Expect = 2.576e-6 Identity = 53/220 (24.09%), Postives = 109/220 (49.55%), Query Frame = 2
Query: 38 ITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAG-PNSNWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWE-SKTELTTELDVLNKQVDLSGISIYLDTK 691
IT +L + KYV+NF ++ + L++G++ +NL L+ VLE+ P+ + SG I +++ +P+T+ S+ I+I I +A P+S K FQ E K +++ +Q D K G S V + +I+ N+ ++ N+ ++Y ++ + S + + + S+ +W+ + ++ V+ K + +S ++I LD +
Sbjct: 8 ITPILLSYVAKYVKNFRDEDAQVSLWEGEVTFQNLDLRLEVLEEELNLPVELVSGHIHELSILVPWTKLMSEPVKIVINTIEFVAKLPDSESKQRRASFQREQRRKS----------KRESVEQPDQTKSPGPASSSSVVN---KIINNINLQCHNIILKYVEDDIVVS------MNVQTLNFSSAGEDWKPTMVDIHPVSVVMRKLLQVSDLTICLDKR 208
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Zebrafish
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:ZFIN;Acc:ZDB-GENE-050912-1]) HSP 1 Score: 867.84 bits (2241), Expect = 0.000e+0 Identity = 775/2719 (28.50%), Postives = 1240/2719 (45.61%), Query Frame = 2
Query: 4142 ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIPPPSP-----------FYKYSKRKKSLSN--SVDIQNCAAFELKLPKNTVKSTEP-----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRF-----------------YDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVN---------EFKFENNDRSR-------------------------------------------------CLLDEMKIDLHEMDVYSAIRIT----DSILK--DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRY--SSFPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIF------TAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRE-NRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPS---------QPNLTALQKLS---KFVDRISIH---HLDDY------------------------------------EFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF---------------------------QERNVAFCDCRK--------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKP---NTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP--LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKN-----AAVFGGFETFKEFYAPLVYKNW-FRPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQS---QLDFNAD--SSTFSNIEMHFSV---EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYA---CVMYEENKSES----PSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLP 11344
+S+++++L D S+ ++ L Q + +F + + + I+LA+ + + +L+ + K L+ S + + +E S SCP + SN+ T +P P FY+ S S+ S C + P S P + L I + ++D++ F T SI R VD DFN L I+++ WV++LDFFG+GS + P++ L D+S + P EIEE +NTKL++ + SL+L +T+ LA S DG++ L GSL L DL+P G +Y +R + L ++++KYG D L RE DM++ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V + QR+AR+ + I AP+LL+P S+ S +++ +LG+ + N F + DR + CLLD + +DL EMD+++A R+ DS K + DL F ++R L E+C+L L V RNL+ +SH PD I+G LSSV S+ +++Y+ IRG+L HNLGE + L+ Y Q DP +T L+G + ++ + + + DV L L+ + + N +L R +F +SKL +ESFS+ SK++ LV + D R SS P + NVF I+ P R+ + QL + Y S D + F V LN ++ +IFDW+Q+ ++++ TAP H + S Q + + P V+ ++ + Q+ K E+ +NV + F ++E+SS L T+A+IL +YK R +RP FS S++ + SC + S+ LSII+PV + + ++C G P+ L D + ++ L ++I A IRLS++D+QL I +S T AN + S+ + D + +K S + LT LQ L + R IH LD E V +C IDDC+D DVPL +I F+ + V +G+ + +F LS DYYN GW P IEPWP + W K + ++ L+ +I+S LL+ T W ++ Q +A+ R + FVP+ + N GC + + L+ + ++ E D+ S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+G+F+R+ D ++ V + I R+V I +GS + I ++S L + N L +P+E + + S+ VVL P + IPL + +Q RP LF+ + + W +V PG+ ++ C S+ F+ Q K I++ N+P K V PGH I + L N LPCD+ + +K + +M +P + + Q+ V L++FP C LLI + ++ V +RLYD RLL L +++ R G + ++IS+ YW+ N+T LPL++ Q +G+T+ AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRSS L F Q + I LP S + FHWPR+D DQLL V+ +D P SGGF+++ SFH+ +R N LR I L+GA +++ F+D D LP P++++N++ + + F+Q+ + R H+ + P + + YA DEP L P + + ++G S D+N LYY+NF+YIA +F D LV DV ++V L +K RSQLW + + L HEGS P K R + L+LD+ LA +S + + +L + D RR Q W F G + + ++ V G E + N RPGSG + V V+ DGPTRV+++ D + S + D N + + F E+ V EG G+ L+N +PEEL++A + +DV + R + L + + IQVDNQ + + CV ++S + P+ NA + + L +F + + +I+EEK+L+K + F E E+ + D ++ ++ D G ++ Y NLK+ + LSV T+ LP DLK +K FP L+ F +A I + Y R H +ET ++ I H++ +L SQA +I GSVDFLGNP+G ++D+T GM L +V +++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ SV T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A + R +PRC LLP
Sbjct: 1219 VSELQVQLSGDLSQGSQG-LVSLRFQDLEGDFTKDHPHSISIQLALHSLLMEDLLEPNPESKYKHLMVSRGAPKPSTFSPKEYLSQSCP--------TASNVLYTEMPRSLPAQFEEAQNVFQFYQRHPSTPSASSRKSKKDAECPSTPPPSPSRQTPSPHPRPDFDDSLVHINVLMVDRRHPEFKTRYGSIGRSVDVDFNCLDILITLQTWVMILDFFGIGSTANNHAVKVPTTTLQPMHEHSPEN-STLNDLSDLYP--------EIEEGVNTKLDLKVHSLSLVLRKKTN-----ELAKASVSKLFTHMEMIDGDLALQGSLGSLSLSDLTPHGDLYRERF--TTRGGEALVYNIYKYGETDFFLQREYDMKVSLQMA-SVQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAVEGQAVRERPQRAARVLLDIEAGAPVLLIPESSRSKRLIVANLGQLRVRNCFLPAGARGTFSLRDKDRVKDVHFTSDAGKTSQKRPTSFSTSSGFMLGKPQPSLGCIPEGDVLGLEDHVCLLDCIALDLQEMDIFAAERLPCEPWDSAGKHLETDLVFPSYSVRRSGESLLKERCHLKLKVERNLDKELSHAVPDMSIHGSLSSVHCSLDLKRYQMIRGLLEHNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVFTSLSLLVDMKDVSLELLDNHKSTGH--NCSLARFDFMKSKLLFESFSNGSKSVNLVSHSLLAHDTRLTGSSAPTEGKHNVFDCILQPS----RTGTNRASLQLELHYRSTRDSSCFTVVLNNLRVFLIFDWLQLVQDFLHMPVEKGTAP---------------THQRWPSGSSDQGCTGAVMPKTVKSGVVTKRSTVPVTQD--KQLEVKINVTGTEFVVVEDSSCLDTNAIILKGTTVLTYKPRLLDRP--FSGSLAGIEVFSCRLGSEQETALSIIDPVNV----QMELC---GSPTYQSSSGLLDAFNIEDFPPL-LEIQFPALDIRLSYNDVQLFLAIAKSIP--TAASANPSDTAVDAESSASTASTDTPSKTKDSFRQKTEALLEGQLTRLQDLGFRKEDCRRALIHCRGQLDQAATWLWENAESMSGRSRTNSGSSSHSAPPLSGVEVKAENVCICFIDDCLDCDVPLAEITFSRLYVLQRIGSMQEGKASFTLSGDYYNRELSGWEPFIEPWPCFLDWQRQAAGRLHPPRLKIGVRAKQRLDVNITSVLLEQYSTTKTSWLADYCKEDEQSPQPSAALSWMGSSVDPPSFGQSLPLAYLRTRSSTSLTCLEQQIHARADMKLSKRRQPFVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISEVHGPGTDDTHNISQWREVLPGEEIPFEFEAREKLRHRH--THELKLHQLLVRVVGWEQVKPVSVDKVGVFFRYAAPDRNNPSSTVGSPISRTNIIHPHVYFSALPPVRVVFSITMEGSARKVITVQSALTVKNRLEVPME-VRLDSPSAPDKPVVLPPILPGEALAIPLHLTSWRLQARPKGLG------------LFFCKVPIH-----WTSVERPGEVSSSKRECQSA--DFD-EQLKRSFRFCVVIKKENYPEQQPAKTVAGGGTKQIYRQPGHTIFLLPTLVLANLLPCDLNYYIKGTSIKGTM---------KPGKEAVLHAADTSQNIEIGVLLENFPVCKELLIPPGTQ-NYVVRMRLYDVNKRLLCLTIRIILRAQGALKILISAPYWLANKTGLPLIFRQDNGKTD--AAGQFEEHELARSLSPLLFCYTDKEQPNMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGINVRKGKGRYRDTHIVTFAPRYLLDNRSSHKLAFSQREFARGKGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCTWSGGFEVNKPKSFHINMRDTLGNC-FFLRAEIALKGATHQISFSDTDQLPPPFRIDNISEVPIQFWQH-GVADIRLHTEVKPGADLDYACDEPILEPYLTVTVKGAGSSEVTADMNFFREYNKLYYENFIYIAASFTFSEDGGKRPVGKNRAVSCAELVLDVETKTQRVILKKKEPGKRSQLWRMTGSGMLCHEGSSPPQS-KPSQPRPLSSSLVLDIAGLAAVTDNSYEPL---MLRRPDSRRSTTQTWHFNSGMLTCGLPRLVVQVSGGVSGLYDGAEVVLGPDSGILEATPEQQFINQKMRPGSGVLSVQVLPDGPTRVLQISDFNQRRVNRPSPSTEEDPNKEIAQTKFPEEELEVVVNLEEGVGLSLVNKLPEELVFATLSGIDVHFTRTAANQVLELTVKNIQVDNQLLGTTHPVLLCVTPGSSESTALDSGPALQVNAVKVPSRLML-----TDLFKHLMVTARRYTVIIEEKLLMKLLIFFGYGQTEGEMEKLD-ENIYEKASEDPGTPKRYYFENLKISLPQVKLSVFTSHKLPHDLKALKGTLGFP-LIRFEDAIINMYPYTRVHPYETQEMVINDILKHFKEELISQAAQILGSVDFLGNPMGLLNDVTEGMNELIKYGNVGGLIRSVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQTERECIRYHGATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASVNNRLSVQRVRKPRCCKGPQGLLP 3809 HSP 2 Score: 119.398 bits (298), Expect = 1.030e-25 Identity = 167/761 (21.94%), Postives = 312/761 (41.00%), Query Frame = 2
Query: 1286 GWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEV-DFEAVNFFTR-DKIFLRGEFSLEGIKLKLLKD-------TEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKV-----PGGE-------------------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEK---GS---SDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS--------LENLSMATDCQASKETKPVT----NIYDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNF--------MNNYRNADSSSVKYKK-----------QTRMDNLNKS--------PEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSESI--------------------------------GDP------IIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKE 3145
GW G+ G ++ + + ++LLP T++ + + +L + + D + +N FTR D +F R +F LE + L + +E ++++ + + ES P+ S + + L LF+RDL + F +VS + V P G+ +F + +E NP+ S +R L +++ PL I++ P I + E+F + + + AA + K +T I++ +++ G + W + L I AP++I P+ ++ +P V++DLGR+ ++N S + ++K + E ESD E+F TP ++P S ++ D + K ++ N+Y++YS+ ++ +VG NW L ES++ ++E FN +L L+++ Q P ++ + L + I+ +K+ + CL + N N ++ D +++++K Q NL KS E+ K + + F ++ + +ESG R ++ L + T + ++S+ + L L D GS D ++ASHK L D P L ES G P +I+ Y+ IS S ++ + + ++LD+I N ET+ ELL F++ F + Q PN E +E
Sbjct: 11 GWGGWYGG-------------TERGPDGQPVSDELLP---GTAQWEILAETDDLFDPLEDSQTLNTFTRRDHLFARLDFKLEKAAVTLYHENKNKPLLSESGVIQLEFSGVKIEVESLPRSESSLLTVKLGGLFLRDLT--TQGSIFPVLVSPKPDKVVVAINQPFGQTNSTEYSNPASDVESSAPPVFEMIYERNPIRS----KFERRLEVNTSPLNIIYNPQAIKKVAEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKHWTMKLDICAPQVIFPD-DFQSEDPMLVVVDLGRILLTN-SQEDHKTNLKTSQTE---GESD---EEFQTPLATPPASPPPEQEVDVKGQEKPRDFSSVKSSEGAQIISRNLYEKYSLSFNDLQIMVGHYKDNWKHLQESDVGPTH-------VVEKFNVLLQLEQRLRYTSDPQFPGAVLSGTLPDLKVHINLEKMTALRSCLARLGNSGADEGDNNGENLKSPDPLTLRHEKIFQREDSDWKLQGSDKNLTKSVMTLEQHTREVLVESKLLLVEFNINYMQLGIESGGRYISVLKVFGTNAHFVKRPYDAMVSLTVHGLYLVDTLQTYGSDFDLLVASHKHLSFDVPTGSLRESQPTSPVSPDNKSPELPGLHRNSSDLAFDKASPRGSPLRDQEALIKLEYQFISSDCPSMNLDSSLQVTSMQVNNLDIILNPETMVELLKFLQQSFPKEESSPTQQIAPLPNGEENEE 734
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Zebrafish
Match: BX005199.2 (vacuolar protein sorting 13 homolog D [Source:NCBI gene;Acc:567879]) HSP 1 Score: 861.677 bits (2225), Expect = 0.000e+0 Identity = 775/2734 (28.35%), Postives = 1241/2734 (45.39%), Query Frame = 2
Query: 4142 ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIPPPSP-----------FYKYSKRKKSLSN--SVDIQNCAAFELKLPKNTVKSTEP-----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRF-----------------YDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVN---------EFKFENNDRSR-------------------------------------------------CLLDEMKIDLHEMDVYSAIRIT----DSILK--DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRY--SSFPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIF------TAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRE-NRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPS---------QPNLTALQKLS---KFVDRISIH---HLDDY------------------------------------EFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF---------------------------QERNVAFCDCRK--------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKP---NTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP--LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKN-----AAVFGGFETFKEFYAPLVYKNW-FRPGSGEVLVSVVTDGPTRVMRLID------------KEDESILSQSQLDFNADS---------STFSNIEMHFS--VEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYA---CVMYEENKSES----PSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLP 11344
+S+++++L D S+ ++ L Q + +F + + + I+LA+ + + +L+ + K L+ S + + +E S SCP + SN+ T +P P FY+ S S+ S C + P S P + L I + ++D++ F T SI R VD DFN L I+++ WV++LDFFG+GS + P++ L D+S + P EIEE +NTKL++ + SL+L +T+ LA S DG++ L GSL L DL+P G +Y +R + L ++++KYG D L RE DM++ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V + QR+AR+ + I AP+LL+P S+ S +++ +LG+ + N F + DR + CLLD + +DL EMD+++A R+ DS K + DL F ++R L E+C+L L V RNL+ +SH PD I+G LSSV S+ +++Y+ IRG+L HNLGE + L+ Y Q DP +T L+G + ++ + + + DV L L+ + + N +L R +F +SKL +ESFS+ SK++ LV + D R SS P + NVF I+ P R+ + QL + Y S D + F V LN ++ +IFDW+Q+ ++++ TAP H + S Q + + P V+ ++ + Q+ K E+ +NV + F ++E+SS L T+A+IL +YK R +RP FS S++ + SC + S+ LSII+PV + + ++C G P+ L D + ++ L ++I A IRLS++D+QL I +S T AN + S+ + D + +K S + LT LQ L + R IH LD E V +C IDDC+D DVPL +I F+ + V +G+ + +F LS DYYN GW P IEPWP + W K + ++ L+ +I+S LL+ T W ++ Q +A+ R + FVP+ + N GC + + L+ + ++ E D+ S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+G+F+R+ D ++ V + I R+V I +GS + I ++S L + N L +P+E + + S+ VVL P + IPL + +Q RP LF+ + + W +V PG+ ++ C S+ F+ Q K I++ N+P K V PGH I + L N LPCD+ + +K + +M +P + + Q+ V L++FP C LLI + ++ V +RLYD RLL L +++ R G + ++IS+ YW+ N+T LPL++ Q +G+T+ AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRSS L F Q + I LP S + FHWPR+D DQLL V+ +D P SGGF+++ SFH+ +R N LR I L+GA +++ F+D D LP P++++N++ + + F+Q+ + R H+ + P + + YA DEP L P + + ++G S D+N LYY+NF+YIA +F D LV DV ++V L +K RSQLW + + L HEGS P K R + L+LD+ LA +S + ++L + D RR Q W F G + + ++ V G E + N RPGSG + V V+ DGPTRV+++ D E++ +Q F + F+NI+ EG G+ L+N +PEEL++A + +DV + R + L + + IQVDNQ + + CV ++S + P+ NA + + L +F + + +I+EEK+L+K + F E E+ + D ++ ++ D G ++ Y NLK+ + LSV T+ LP DLK +K FP L+ F +A I + Y R H +ET ++ I H++ +L SQA +I GSVDFLGNP+G ++D+T GM L +V +++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ SV T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A + R +PRC LLP
Sbjct: 1219 VSELQVQLSGDLSQGSQG-LVSLRFQDLEGDFTKDHPHSISIQLALHSLLMEDLLEPNPESKYKHLMVSRGAPKPSTFSPKEYLSQSCP--------TASNVLYTEMPRSLPAQFEEAQNVFQFYQRHPSTPSASSRKSKKDAECPSTPPPSPSRQTPSPHPRPDFDDSLVHINVLMVDRRHPEFKTRYGSIGRSVDVDFNCLDILITLQTWVMILDFFGIGSTANNHAVKVPTTTLQPMHEHSPEN-STLNDLSDLYP--------EIEEGVNTKLDLKVHSLSLVLRKKTN-----ELAKASVSKLFTHMEMIDGDLALQGSLGSLSLSDLTPHGDLYRERF--TTRGGEALVYNIYKYGETDFFLQREYDMKVSLQMA-SVQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAVEGQAVRERPQRAARVLLDIEAGAPVLLIPESSRSKRLIVANLGQLRVRNCFLPAGARGTFSLRDKDRVKDVHFTSDAGKTSQKRPTSFSSSSGFVLGKPQPSLGCIPEGDVLGLEDHVCLLDCIALDLQEMDIFAAERLPCEPWDSAGKHLETDLVFPSYSVRRSGESLLKERCHLKLKVERNLDKELSHAVPDMSIHGSLSSVHCSLDLKRYQMIRGLLEHNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVFTSLSLLVDMKDVSLELLDNHKSTGH--NCSLARFDFMKSKLLFESFSNGSKSVNLVSHSLLAHDTRLTGSSAPTEGKHNVFDCILQPS----RTGTNRASLQLELHYRSTRDSSCFTVVLNNLRVFLIFDWLQLVQDFLHMPVEKGTAP---------------THQRWPSGSSDQGCTGAVMPKTVKSGVVTKRSTVPVTQD--KQLEVKINVTGTEFVVVEDSSCLDTNAIILKGTTVLTYKPRLLDRP--FSGSLAGIEVFSCRLGSEQETALSIIDPVNV----QMELC---GSPTYQSSSGLLDAFNIEDFPPL-LEIQFPALDIRLSYNDVQLFLAIAKSIP--TAASANPSDTAVDAESSASTASTDTPSKTKDSFRQKTEALLEGQLTRLQDLGFRKEDCRRALIHCRGQLDQAATWLWENAESMSGRSRTNSGSSSHSAPPLSGVEVKAESVCICFIDDCLDCDVPLAEITFSRLYVLQRIGSMQEGKASFTLSGDYYNRELSGWEPFIEPWPCFLDWQRQAAGRLHPPRLKIGVRAKQRLDVNITSVLLEQYSTTKTSWLADYCKEDEQSPQPSAALSWMGSSVDPPSFGQSLPLAYLRTRSSTSLTCLEQQIHARADMKLSKRRQPFVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISEVHGPGTDDTHNISQWREVLPGEEIPFEFEAREKLRHRH--THELKLHQLLVRVVGWEQVKPVSVDKVGVFFRYAAPDRNNPSSTVGSPISRTNIIHPHVYFSALPPVRVVFSITMEGSARKVITVQSALTVKNRLEVPME-VRLDSPSAPDKPVVLPPILPGEALAIPLHLTSWRLQARPKGLG------------LFFCKVPIH-----WTSVERPGEVSSSKRECQSA--DFD-EQLKRSFRFCVVIKKENYPEQQPAKTVAGGGTKQIYRQPGHTIFLLPTLVLANLLPCDLNYYIKGTSIKGTM---------KPGKEAVLHAADTSQNIEIGVLLENFPVCKELLIPPGTQ-NYVVRMRLYDVNKRLLCLTIRIILRAQGALKILISAPYWLANKTGLPLIFRQDNGKTD--AAGQFEEHELARSLSPLLFCYTDKEQPNMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGINVRKGKGRYRDTHIVTFAPRYLLDNRSSHKLAFSQREFARGKGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCTWSGGFEVNKPKSFHINMRDTLGN-CFFLRAEIALKGATHQISFSDTDQLPPPFRIDNISEVPIQFWQH-GVADIRLHTEVKPGADLDYACDEPILEPYLTVTVKGAGSSEVTADMNFFREYNKLYYENFIYIAASFTFSEDGGKRPVGKNRAVSCAELVLDVETKTQRVILKKKEPGKRSQLWRMTGSGMLCHEGSSPPQS-KPSQPRPLSSSLVLDIAGLAAVTDNSYEP---LMLRRPDSRRSTTQTWHFNSGMLTCGLPRLVVQVSGGVSGLYDGAEVVLGPDSGILEATPEQQFINQKMRPGSGVLSVQVLPDGPTRVLQISDFNQRRVNRPSPSTEEDPNKEIAQTKFPEEELEVKTMFFFVKFANIDYVVVNLEEGVGLSLVNKLPEELVFATLSGIDVHFTRTAANQVLELTVKNIQVDNQLLGTTHPVLLCVTPGSSESTALDSGPALQVNAVKVPSRLML-----TDLFKHLMVTARRYTVIIEEKLLMKLLIFFGYGQTEGEMEKLD-ENIYEKASEDPGTPKRYYFENLKISLPQVKLSVFTSHKLPHDLKALKGTLGFP-LIRFEDAIINMYPYTRVHPYETQEMVINDILKHFKEELISQAAQILGSVDFLGNPMGLLNDVTEGMNELIKYGNVGGLIRSVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQTERECIRYHGATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASVNNRLSVQRVRKPRCCKGPQGLLP 3824 HSP 2 Score: 119.398 bits (298), Expect = 1.102e-25 Identity = 167/761 (21.94%), Postives = 312/761 (41.00%), Query Frame = 2
Query: 1286 GWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEV-DFEAVNFFTR-DKIFLRGEFSLEGIKLKLLKD-------TEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKV-----PGGE-------------------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEK---GS---SDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS--------LENLSMATDCQASKETKPVT----NIYDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNF--------MNNYRNADSSSVKYKK-----------QTRMDNLNKS--------PEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSESI--------------------------------GDP------IIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKE 3145
GW G+ G ++ + + ++LLP T++ + + +L + + D + +N FTR D +F R +F LE + L + +E ++++ + + ES P+ S + + L LF+RDL + F +VS + V P G+ +F + +E NP+ S +R L +++ PL I++ P I + E+F + + + AA + K +T I++ +++ G + W + L I AP++I P+ ++ +P V++DLGR+ ++N S + ++K + E ESD E+F TP ++P S ++ D + K ++ N+Y++YS+ ++ +VG NW L ES++ ++E FN +L L+++ Q P ++ + L + I+ +K+ + CL + N N ++ D +++++K Q NL KS E+ K + + F ++ + +ESG R ++ L + T + ++S+ + L L D GS D ++ASHK L D P L ES G P +I+ Y+ IS S ++ + + ++LD+I N ET+ ELL F++ F + Q PN E +E
Sbjct: 11 GWGGWYGG-------------TERGPDGQPVSDELLP---GTAQWEILAETDDLFDPLEDSQTLNTFTRRDHLFARLDFKLEKAAVTLYHENKNKPLLSESGVIQLEFSGVKIEVESLPRSESSLLTVKLGGLFLRDLT--TQGSIFPVLVSPKPDKVVVAINQPFGQTNSTEYSNPASDVESSAPPVFEMIYERNPIRS----KFERRLEVNTSPLNIIYNPQAIKKVAEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKHWTMKLDICAPQVIFPD-DFQSEDPMLVVVDLGRILLTN-SQEDHKTNLKTSQTE---GESD---EEFQTPLATPPASPPPEQEVDVKGQEKPRDFSSVKSSEGAQIISRNLYEKYSLSFNDLQIMVGHYKDNWKHLQESDVGPTH-------VVEKFNVLLQLEQRLRYTSDPQFPGAVLSGTLPDLKVHINLEKMTALRSCLARLGNSGADEGDNNGENLKSPDPLTLRHEKIFQREDSDWKLQGSDKNLTKSVMTLEQHTREVLVESKLLLVEFNINYMQLGIESGGRYISVLKVFGTNAHFVKRPYDAMVSLTVHGLYLVDTLQTYGSDFDLLVASHKHLSFDVPTGSLRESQPTSPVSPDNKSPELPGLHRNSSDLVFDKASPRGSPLRDQEALIKLEYQFISSDCPSMNLDSSLQVTSMQVNNLDIILNPETMVELLKFLQQSFPKEESSPTQQIAPLPNGEENEE 734
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Zebrafish
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:ZFIN;Acc:ZDB-GENE-050912-1]) HSP 1 Score: 761.525 bits (1965), Expect = 0.000e+0 Identity = 637/2178 (29.25%), Postives = 1014/2178 (46.56%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRIT----DSILK--DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRY--SSFPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRE-NRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPS---------QPNLTALQKLS---KFVDRISIH---HLDDY------------------------------------EFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF---------------------------QERNVAFCDCRK--------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKP---NTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP--LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKN-----AAVFGGFETFKEFYAPLVYKNW-FRPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQS---QLDFNAD--SSTFSNIEMHFSV---EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYA---CVMYEENKSES----PSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLP 11344
CLLD + +DL EMD+++A R+ DS K + DL F ++R L E+C+L L V RNL+ +SH PD I+G LSSV S+ +++Y+ IRG+L HNLGE + L+ Y Q DP +T L+G + ++ + + + DV L L+ + + N +L R +F +SKL +ESFS+ SK++ LV + D R SS P + NVF I+ P R+ + QL + Y S D + F V LN ++ +IFDW+Q+ ++++ ++ H + S Q + + P V+ ++ + Q+ K E+ +NV + F ++E+SS L T+A+IL +YK R +RP FS S++ + SC + S+ LSII+PV + + ++C G P+ L D + ++ L ++I A IRLS++D+QL I +S T AN + S+ + D + +K S + LT LQ L + R IH LD E V +C IDDC+D DVPL +I F+ + V +G+ + +F LS DYYN GW P IEPWP + W K + ++ L+ +I+S LL+ T W ++ Q +A+ R + FVP+ + N GC + + L+ + ++ E D+ S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+G+F+R+ D ++ V + I R+V I +GS + I ++S L + N L +P+E + + S+ VVL P + IPL + +Q RP LF+ + + W +V PG+ ++ C S+ F+ Q K I++ N+P K V PGH I + L N LPCD+ + +K + +M +P + + Q+ V L++FP C LLI + ++ V +RLYD RLL L +++ R G + ++IS+ YW+ N+T LPL++ Q +G+T+ AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRSS L F Q + I LP S + FHWPR+D DQLL V+ +D P SGGF+++ SFH+ +R N LR I L+GA +++ F+D D LP P++++N++ + + F+Q+ + R H+ + P + + YA DEP L P + + ++G S D+N LYY+NF+YIA +F D LV DV ++V L +K RSQLW + + L HEGS P K R + L+LD+ LA +S + + +L + D RR Q W F G + + ++ V G E + N RPGSG + V V+ DGPTRV+++ D + S + D N + + F E+ V EG G+ L+N +PEEL++A + +DV + R + L + + IQVDNQ + + CV ++S + P+ NA + + L +F + + +I+EEK+L+K + F E E+ + D ++ ++ D G ++ Y NLK+ + LSV T+ LP DLK +K FP L+ F +A I + Y R H +ET ++ I H++ +L SQA +I GSVDFLGNP+G ++D+T GM L +V +++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ SV T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A + R +PRC LLP
Sbjct: 5 CLLDCIALDLQEMDIFAAERLPCEPWDSAGKHLETDLVFPSYSVRRSGESLLKERCHLKLKVERNLDKELSHAVPDMSIHGSLSSVHCSLDLKRYQMIRGLLEHNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVFTSLSLLVDMKDVSLELLDNHKSTGH--NCSLARFDFMKSKLLFESFSNGSKSVNLVSHSLLAHDTRLTGSSAPTEGKHNVFDCILQPS----RTGTNRASLQLELHYRSTRDSSCFTVVLNNLRVFLIFDWLQLVQDFLHMPV---------EKGTAPTHQRWPSGSSDQGCTGAVMPKTVKSGVVTKRSTVPVTQD--KQLEVKINVTGTEFVVVEDSSCLDTNAIILKGTTVLTYKPRLLDRP--FSGSLAGIEVFSCRLGSEQETALSIIDPVNV----QMELC---GSPTYQSSSGLLDAFNIEDFPPL-LEIQFPALDIRLSYNDVQLFLAIAKSIP--TAASANPSDTAVDAESSASTASTDTPSKTKDSFRQKTEALLEGQLTRLQDLGFRKEDCRRALIHCRGQLDQAATWLWENAESMSGRSRTNSGSSSHSAPPLSGVEVKAENVCICFIDDCLDCDVPLAEITFSRLYVLQRIGSMQEGKASFTLSGDYYNRELSGWEPFIEPWPCFLDWQRQAAGRLHPPRLKIGVRAKQRLDVNITSVLLEQYSTTKTSWLADYCKEDEQSPQPSAALSWMGSSVDPPSFGQSLPLAYLRTRSSTSLTCLEQQIHARADMKLSKRRQPFVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISEVHGPGTDDTHNISQWREVLPGEEIPFEFEAREKLRHRH--THELKLHQLLVRVVGWEQVKPVSVDKVGVFFRYAAPDRNNPSSTVGSPISRTNIIHPHVYFSALPPVRVVFSITMEGSARKVITVQSALTVKNRLEVPME-VRLDSPSAPDKPVVLPPILPGEALAIPLHLTSWRLQARPKGLG------------LFFCKVPIH-----WTSVERPGEVSSSKRECQSA--DFD-EQLKRSFRFCVVIKKENYPEQQPAKTVAGGGTKQIYRQPGHTIFLLPTLVLANLLPCDLNYYIKGTSIKGTM---------KPGKEAVLHAADTSQNIEIGVLLENFPVCKELLIPPGTQ-NYVVRMRLYDVNKRLLCLTIRIILRAQGALKILISAPYWLANKTGLPLIFRQDNGKTD--AAGQFEEHELARSLSPLLFCYTDKEQPNMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGINVRKGKGRYRDTHIVTFAPRYLLDNRSSHKLAFSQREFARGKGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCTWSGGFEVNKPKSFHINMRDTLGNC-FFLRAEIALKGATHQISFSDTDQLPPPFRIDNISEVPIQFWQH-GVADIRLHTEVKPGADLDYACDEPILEPYLTVTVKGAGSSEVTADMNFFREYNKLYYENFIYIAASFTFSEDGGKRPVGKNRAVSCAELVLDVETKTQRVILKKKEPGKRSQLWRMTGSGMLCHEGSSPPQS-KPSQPRPLSSSLVLDIAGLAAVTDNSYEPL---MLRRPDSRRSTTQTWHFNSGMLTCGLPRLVVQVSGGVSGLYDGAEVVLGPDSGILEATPEQQFINQKMRPGSGVLSVQVLPDGPTRVLQISDFNQRRVNRPSPSTEEDPNKEIAQTKFPEEELEVVVNLEEGVGLSLVNKLPEELVFATLSGIDVHFTRTAANQVLELTVKNIQVDNQLLGTTHPVLLCVTPGSSESTALDSGPALQVNAVKVPSRLML-----TDLFKHLMVTARRYTVIIEEKLLMKLLIFFGYGQTEGEMEKLD-ENIYEKASEDPGTPKRYYFENLKISLPQVKLSVFTSHKLPHDLKALKGTLGFP-LIRFEDAIINMYPYTRVHPYETQEMVINDILKHFKEELISQAAQILGSVDFLGNPMGLLNDVTEGMNELIKYGNVGGLIRSVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQTERECIRYHGATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASVNNRLSVQRVRKPRCCKGPQGLLP 2086
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Zebrafish
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:ZFIN;Acc:ZDB-GENE-050912-1]) HSP 1 Score: 284.263 bits (726), Expect = 7.890e-76 Identity = 300/1216 (24.67%), Postives = 538/1216 (44.24%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFE-KEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKS---DCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKG--------AALRERARNLVK--YVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAM------EHIGRKSRDLLQN-------AVNIPAPQQSGWWGYL------WGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEV-DFEAVNFFT-RDKIFLRGEFSLEGIKLKLLKD-------TEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKV-----PGGE-------------------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEK---G---SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS--------LENLSMATDCQASKETKPVT----NIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNF--------MNNYRNADSSSVKYKK-----------QTRMDNLNKS--------PEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSESI--------------------------------GDP------IIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKE 3145
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L F+ P VK+G+IG++TL+IP+ R S W I + + +I GP + E +E + E KK LL ++E KW+K G++ W S AS+ RI+ N++++++ VH+R+ED+ + P + +A G+ I S ++ E K + K++++S S+Y DT+C ++ ++ S +QE++++ +S I P+ +++RN + PL + PRI +Q+E + + LSQ QY+ +A E +R +++ K+RP++++ + ++WW+FAI + I K A R R L YVQ + L+ E E +E + L+ L LRE E I R+ L N + +IP SG YL WG T + + + ++LLP T++ + + +L + + D + +N FT RD +F R +F LE + L + +E ++++ + + ES P+ S + + L LF+RDL + F +VS + V P G+ +F + +E NP+ S +R L +++ PL I++ P I + E+F + + + AA + K +T I++ +++ G + W + L I AP++I P+ ++ +P V++DLGR+ ++N S + ++K + E ESD E+F TP ++P S ++ D + K ++ N+Y++YS+ ++ +VG NW L+ T ++E FN +L L+++ Q P ++ + L + I+ +K+ + CL + N N ++ D +++++K Q NL KS E+ K + + F ++ + +ESG R ++ L + T + ++S+ + L L D GS D ++ASHK L D P L ES G P +I+ Y+ IS S ++ + + ++LD+I N ET+ ELL F++ F + Q PN E +E
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRG-FDLPFEVKAGVIGKITLQIPFYRPHSDPWVICMSQLNLIIGPALPQEYDEVQEREAEREQKKQLLKALEDKWKK-----GES------YWYSVTASVVTRIVENIELKIQGVHLRFEDDFSNPEKPYAFGVCIKNVSAQNCSKEPAQKL-------IRQKELEISEFSVYWDTECTMLGDLP----SSEIQERMSKCMQSREHQYIFEPVCASVLVRRNASKEPLRSRNTPRIEGQVQLEPLSLRLSQVQYQQIMAFLKELDRREKEMLFRKWRPKLTISGNCRQWWMFAINANLHEIRKQRRHGSWDFALHRARDAKLYTSLYVQRLKGVTLSPQEESEME---RIEDEQTLEELQILRETVYVSFRKHEEIAESLREPLINESPATSPSGSIPKSGSSGMIQYLQSWFPGWGGWYGGT--------ERGPDGQPVSDELLP---GTAQWEILAETDDLFDPLEDSQTLNTFTRRDHLFARLDFKLEKAAVTLYHENKNKPLLSESGVIQLEFSGVKIEVESLPRSESSLLTVKLGGLFLRDLT--TQGSIFPVLVSPKPDKVVVAINQPFGQTNSTEYSNPASDVESSAPPVFEMIYERNPIRS----KFERRLEVNTSPLNIIYNPQAIKKVAEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKHWTMKLDICAPQVIFPD-DFQSEDPMLVVVDLGRILLTN-SQEDHKTNLKTSQTE---GESD---EEFQTPLATPPASPPPEQEVDVKGQEKPRDFSSVKSSEGAQIISRNLYEKYSLSFNDLQIMVGHYKDNWKHLQESDVGPTH------VVEKFNVLLQLEQRLRYTSDPQFPGAVLSGTLPDLKVHINLEKMTALRSCLARLGNSGADEGDNNGENLKSPDPLTLRHEKIFQREDSDWKLQGSDKNLTKSVMTLEQHTREVLVESKLLLVEFNINYMQLGIESGGRYISVLKVFGTNAHFVKRPYDAMVSLTVHGLYLVDTLQTYGSDFDLLVASHKHLSFDVPTGSLRESQPTSPVSPDNKSPELPGLHRNSSDLAFDKASPRGSPLRDQEALIKLEYQFISSDCPSMNLDSSLQVTSMQVNNLDIILNPETMVELLKFLQQSFPKEESSPTQQIAPLPNGEENEE 1159 HSP 2 Score: 133.265 bits (334), Expect = 4.823e-30 Identity = 133/468 (28.42%), Postives = 221/468 (47.22%), Query Frame = 2
Query: 4142 ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIPPPSP-----------FYKYSKRKKSLSN--SVDIQNCAAFELKLPKNTVKSTEP-----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRF-----------------YDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEF 5437
+S+++++L D S+ ++ L Q + +F + + + I+LA+ + + +L+ + K L+ S + + +E S SCP + SN+ T +P P FY+ S S+ S C + P S P + L I + ++D++ F T SI R VD DFN L I+++ WV++LDFFG+GS + P++ L D+S + P EIEE +NTKL++ + SL+L +T+ LA S DG++ L GSL L DL+P G +Y +R + L ++++KYG D L RE DM++ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V + QR+AR+ + I AP+LL+P S+ S +++ +LG+ + N F
Sbjct: 1644 VSELQVQLSGDLSQGSQG-LVSLRFQDLEGDFTKDHPHSISIQLALHSLLMEDLLEPNPESKYKHLMVSRGAPKPSTFSPKEYLSQSCP--------TASNVLYTEMPRSLPAQFEEAQNVFQFYQRHPSTPSASSRKSKKDAECPSTPPPSPSRQTPSPHPRPDFDDSLVHINVLMVDRRHPEFKTRYGSIGRSVDVDFNCLDILITLQTWVMILDFFGIGSTANNHAVKVPTTTLQPMHEHSPEN-STLNDLSDLYP--------EIEEGVNTKLDLKVHSLSLVLRKKTN-----ELAKASVSKLFTHMEMIDGDLALQGSLGSLSLSDLTPHGDLYRERF--TTRGGEALVYNIYKYGETDFFLQREYDMKVSLQMAS-VQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAVEGQAVRERPQRAARVLLDIEAGAPVLLIPESSRSKRLIVANLGQLRVRNCF 2085
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Zebrafish
Match: vps13a (vacuolar protein sorting 13 homolog A [Source:ZFIN;Acc:ZDB-GENE-030926-1]) HSP 1 Score: 147.517 bits (371), Expect = 2.709e-34 Identity = 160/728 (21.98%), Postives = 325/728 (44.64%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKL---NEMPKS-DCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDC--LLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLW-------GRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTE-IPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYF----SRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKIS--APKIILPNFRLKTHEPSCVLLDLGRLQVSNCS 2119
++ ++ +L+R +G YV N + + G++ GD L NL +K L L + P V++G IGR+ L+IP+ +Q+ ++++Y++ P ++ K EKE + ++ L IE +K + + +G+ ++V L ++++NLQ+++ N+HVRYED+ T PS + G+ + S +++D W + L + + K V L + Y + ++ S + E L+ + +P+ I PIS + ++ N P++ L + + +V + LS+ QY + L + R + KYRP V V + ++WW + IT + H++ +R +++ KY + Y++ +++K E+ + E++ LD + R+ A + + + V + Q GW+G++W ++K+ D +E +++ + SE + ++ + +FE ++ F L + L + + E I++++ +L RP + I L S V L + S ++S R + + L FETNPL + ++ I S+PL I++ + + ++ +F + + A + + +TS + I+E + + LKI+ A +I+P + + +LDLG Q+S+ S
Sbjct: 1 MVFESVVVDVLNRFLGDYVGNLDSSQLSLGIWGGDAILRNLEIKENALSQL-DIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEERQLQESRQRELQRIEETKQKA----AEKENPKGEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPSVPLSFGISLQNLSLQTADQNW-NPCLLDEKAKLFFKLVRLDNLFAYWNVSSELYSNHNPEEALRYLKHSVAIQTAIPQDYHFIFRPISANAKLRMNPRSDVDFSSPKMDLVVNLPEVAVELSRPQYVSILELLGSVDMMTRNLPYRKYRPYVVVHTNARQWWRYVITGVLEVNVKPRLHMWSWLHIRRHRQHVKKYRELYKNKIISKKPA-ESLLKELEETERTLDIFNITMARQQAEMEASKAGLRIYRPGVKVEEEQSQGWFGWMWNWSGEASATQTKDVKTGGFDGLMTPAEKAKLYAAI-----GYSETAVNPNLPK-----NFEN----------MKMNFHLLRLSLSIKESREKAEIIKLSVIDLKAMLTQRPAAQAIKISAQLSSFEVSGLP---REKSAPILLSPRHVDGMKDKSLLNLMFETNPLDARADQRLR----IESQPLEIIYDAMTVNSMSAFFMPPKDLQLDELTNATLMKLEQFRDKTSTGLMYIIE-----TQKVLDLKINLMASYVIVPENGFYDPKHNLAILDLGHFQMSSQS 689 HSP 2 Score: 93.2041 bits (230), Expect = 7.944e-18 Identity = 99/423 (23.40%), Postives = 175/423 (41.37%), Query Frame = 2
Query: 10241 VIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLK----FQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMF-------LSPSKEAELLEEDF----SSLQHQSHFDEG-----RKIYIGNLKLLTGVI-------------SLSVITAESLPSDLKQMKKLFPSLMSFTNAQIII---DVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSD--QDVYNMVKHFAHGIG---------------DSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPYQ 11350
V I +IQ+ NQ A + V Y +S S L R GH D+ + + I M + L+ L +++F ++ +E EL ++D + L H S D +I +KL + +I +SL LK + + T+AQ ++ ++ + F T + + HY Q Q + +D LGNP G I ++ G++ + + Q + F G+ + ++I + ++ ++ +TMD EY++KR+ N + SG + G K L G GI + T+ KG + EG +G F G KG+VG +T+P GG++D+A+ + + A S++ + R PR + + PY+
Sbjct: 2685 VQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHS-DILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDTSPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGA------TLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAM-NKQPSGLREGLTRGG-KGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLR--PPRFIHEDGVIRPYR 3096 HSP 3 Score: 62.003 bits (149), Expect = 1.935e-8 Identity = 41/160 (25.62%), Postives = 79/160 (49.38%), Query Frame = 2
Query: 5309 AQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF---ENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTD 5779
A+RS RI + + +AP++ LP S+ S ++++ DLG ++ N F+ + + ++D M + L ++ +Y D + L ++P+ N++L++ RNL+ + H+ PD QI L + +S E + L+ NL E +D
Sbjct: 1230 AERSTRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQL------LKPV---------NMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSETSD 1374
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Xenopus
Match: trim13 (tripartite motif containing 13 [Source:Xenbase;Acc:XB-GENE-961103]) HSP 1 Score: 600.127 bits (1546), Expect = 2.997e-171 Identity = 466/1567 (29.74%), Postives = 747/1567 (47.67%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICW-------FWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--QERNVAFCDCRK-------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKC-----GDEN---------KWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQ--YNCNEDQNKP---IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDL--DNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPL-----------VYKNWFRPGSGEVLVSVVTDGPTRVMRLID---KEDESILSQSQLDFNADSSTFSNIEMHFSVE-------GFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY------EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V VC IDDC+D DVPL ++ F+ ++ L N KG + LS DYYN GW P IEPWP ++ W + K + ++ L+ +I+S L+ +T + W ++ QE+ + + FVPF + N GC + + L+ P + ++ GD+N +W V + F+ ET R+K+R+ +H H++++++ GW PV+VDK+GIF+R+ D + V + +R+V + +GS + + IRS L + N L IP+E + ++ + P IPL + +Q RP + F +PI W VN G+ ++ C S + E ++ + C I++ N+P P + PGH I + + N LPC++ F +K + + ++P + + T Q+ V L++F C LLI + ++ V +RLYD RLL L +++ R G + + +S+ YW++N+T LPL++ Q ++T AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG++++ GRGK+ +T MV RYLL N+S++ L F Q + + N P I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITLQ A YR+ FT+ D LP P++++N + + V F Q+ + R + + + + YALDEPTLP I + ++G S ++N + LYY+NF+YIA +F + + LV DV P +V L +K RSQLW + L HEGS APH+P K + RS D+ L+LD+ LA + + + ++L K D RR Q W F +G + + G+ + A + ++ G E A L RPGSG + V V+ DGPTRV+++ D + ++ + + + N E+ +E G G+ L+N +PEEL++ + +++ + + L + + +QVDNQ + +++ E + P+ +A + + +L +F + + + +EEK+LLK + F E + E + H +E + + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + + H +ET I I H++ +L SQA KI GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC +S LLP
Sbjct: 2615 ESVCVCFIDDCMDCDVPLAELTFSRLSYFQHLVSNPKGFAEFILSGDYYNRELSGWEPFIEPWPCSLSWQQQEASRLHPSRLKLEMKAKQLLDINITSVLIDQYISTKEAWLADYCKQEKECEIDTSEEWMGSSVDPPCLGPTEVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPEVVPDGDKNLIDDTHNVSQWREVLPG--QEIPFEFET--REKLRHRH--THDLRIHQLQVRVSGWEPISPVSVDKVGIFFRYAAPDKNTSSSSVGSPISKTNIIHPQVYFSALPPVRVVFAVTMEGSARKVVTIRSSLMVKNKLEIPMELRLDSPSAPDKPVILPAVMPGESFAIPLHLTSWRLQARP-----------RGMGVFFCKAPIH-------WTNVNKAGEVSSSKRECHSMDA------EISRYFRFCVAIKKDNYPDYVPSNIFSDSAKQIYRQPGHTIFLLPTVVICNLLPCELNFYVK---------GLPIKGTLKPGREMALHTADTSQNFELGVCLENFQHCKELLIPPGTQ-NYMVRMRLYDGHKRLLNLTIRIACRAEGSLKIFVSAPYWLINKTGLPLIFRQDN-SKTDAAGQFEEHELARSLSPLLFCYADKELPNLCTMRIGRGIHPEGI----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGKYRDTCMVTFAPRYLLINKSTYKLAFAQREFARGQGTNNPVGYISTLPGSSVVFHWPRNDYDQLLCVRLMD---LPNCVWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLQSATYRISFTNTDQLPPPFRIDNFSKVPVVFVQH-GVADPRLRTEVKSATSMDYALDEPTLPSYIAVTVKGAGSSEITCNMNDFQDSKQLYYENFIYIAASYTFSGYVITNAELVLDVHPKTLRVVLKKKEPGKRSQLWRMTGTGMLCHEGSSAPHNPNKPQSTRSLDSSLVLDIAGLA---AVTDNKYEPLMLRKPDHRRSTTQTWCFRDGKLTCSLHGL--VVQARNGTSGLYDGAEVVLGPDASLDLLGSVPKEQQFINQKMRPGSGVLSVRVIPDGPTRVLQITDLSQRRSNRFSNEIEELAELELRKYKNPEVKQEIEVLVKLQDGIGISLVNKVPEELVFVSLSGINIHYTHLTVNQVLELSVQNVQVDNQLIGTTQPTMLFVTPSSIESEEEIGPAIQVSAMMVPSKNKLST-----LFKHLLIKAQKFTVQIEEKLLLKLLSYFGFDQSETGV--EKWDENIHDKPSEENKSQQRYYFENLKISIPQIKLSVFTSNKLPPDLKALKSTLGFP-LIKFEDAVIDLDPFAKVHPYETQEFIINDIIKHFQEELLSQAAKILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQTEREYIRYHAATSGEHLV---AGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGFGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGHRMQVQRIRKPRCCTSPQGLLP 4108 HSP 2 Score: 301.982 bits (772), Expect = 9.580e-81 Identity = 250/821 (30.45%), Postives = 403/821 (49.09%), Query Frame = 2
Query: 4586 NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRF------YDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTK--DGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF-----------ENNDRSR-----------------------------------CLLDEMKIDLHEMDVYSAIRITDSILKD-----GDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENT-DHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFP--ANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISF-----QPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
+ L I + L+DKK FS+ I+R VD DFN L I+++ WV++LDFFG+GS P D + +P + + E NTKL++ + SL+L+N + +N++ L +Y + +G++ L GS+ L DL+ G +Y +R + + L F++FKYGLPD +L RE DMR+ +++ + Y+HTQRF EL F Q+F Q QD L R G+ V AQRS+RI + I AP+LL+P S++S +++ +LGK + N+F F +N+ R CLLD + +DL +MD+++A R + + DL F +R + L+E+C + L + RNL+ ISH PD I+G LSSV S+ + Y+ IRG+L +NLGE D ++ Y Q DP +HT L+G Y ++ + + +V L L+ +G D N +L R +F +SK +ESFS+ +K+I LV + D R++ A + NVF I+ P K + + Q+ + + S D + F V LN ++ +IFDW+ + + L T E++ H + + KP + S V+ ++ +II E K E+ +NV + F ++E+ S T+A+IL +YK + +R PFS S+ + SC + ++ LSII+PV I I L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 1732 DSLVHINVFLVDKKHPEFSSLYKRINRSVDVDFNCLDILITLQTWVVILDFFGIGSTADNHAVKQQPSDTRQAPKSETNMPLVSEK------EHTNTKLDLKVHSLSLVLKNTRSELAKANVS-------KLTSYLEMIEGDLALQGSIGSLSLSDLTVHGEVYTERF--TTKGEEALIFYIFKYGLPDLALKREYDMRVSLRMA-SVQYVHTQRFQAELVAFIQHFTQLQDVLGRQRAAIQGQTVRNQAQRSSRILLDIEAGAPVLLIPESSWSSKLIVANLGKLRVRNKFLFAGTPETFSLKDKNSQFKRKKKKLMDDLNFKEPLAMVMYSNINIFYSSFTEEHICLLDCIIVDLQDMDIFAAERFSMDFREGFDDFRADLIFPSYFVRQVGGSLLTERCRMKLQLERNLDKEISHAVPDLSIHGNLSSVHCSLDLSDYKLIRGLLENNLGEPVEDFMRPYDLQ----DPKIHTVLSGDVYTSVSFLIDMINVSLELLEE-KGKDGLNN-SLARFDFKKSKFLFESFSNQTKSINLVSHSMMAYDTRFAGQKPLAAHKPNVFNCILQPAKTS----SNPGSLQIELHHRSTKDTSCFTVVLNNLRVFLIFDWLMLVHKF----------LQTPAESKKHVHSSSSEEQKPSNASPVIPKTVKSGVVTKRSSVQIIPE--KQLEVKINVTGTEFVVVEDMSSCDTNAIILKGTTVLTYKPKLADR--PFSGSLFGIEVFSCRVGNELDTALSIIDPVHIQIDLVGN-SAYQNSSGLMDAFNTEDFPPI-LEIQLPALDIRLSYNDVQLFLAIAKSIPKQA 2510 HSP 3 Score: 297.745 bits (761), Expect = 1.552e-79 Identity = 327/1440 (22.71%), Postives = 634/1440 (44.03%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFE-KEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAA------LRERARNLVKYVQSYEDYLLN-KDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEV-DFEAVNFFT-RDKIFLRGEFSLEGIKLKLL-------KDTEIPILEVN-AKNLIVGFESRPQYSSWCIRIGLRSLFVRDLN------PD--YKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS-LENLSMATDCQASKETKPV------------TNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKL---SRYSSM----------------TIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP-----------------------------------LSESIGD--PIIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQ-----------KPETPNTTEFKEFDVSIACKAVSILLIKISSNLASEN-RFAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTT-------GSNETKMEISENEDVIN-------ISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAIL--SSEESTRIGF--------------------------LNSVK--LHLRMLSPILALPRHKTSNQIILVNLGDIEITSDTQSLQVAFSDAIVI 3802
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+G IG++ ++IP+ R W I I +++I + E KE E KK LL ++E KW+ ++G++ W S AS+ RI+ N++++V++VH+R+ED T PS FA G+ I + S +++ E E + KQ+D++ S+Y D ++ ++ ++ + + + E I+ PI ++KRN + PL HQPRI + +E + + LSQ QY+ V DEFER +RQ K++P V V ++ KEWW+FA+ + I + + RAR+ + Y + D + + +N E + +E + + L L+E+ E +K ++ +N + P S +SP ++ +S Q L+ P E + + + D +N FT RD +F + LEG + LL + E P +++ + NL ES P+ S + I L LF+RDL PD Y N + S E +F + +E NP+ S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ +++ + RW V L ISAP++I P+ + +P V++DLGR+ ++N D + S +V +D E++ TP ++P S ++ + +A TK V +Y++YS+ ++ +VG NW + + P ++ E FN +L L+++ + P I+ + L I ++++K+ + C +TN + + +D++ K K +N + + N ++ H+ + +ES LAE + Q + +Y S+ +++ + L++ D GS D ++ASHK L D P SE++ D +I+ Y+ +S + S ++ + ++ ++LD+I N ET+ EL+SF++ F +++++ Q + ET +T + +++ ++ILL + + EN ++ I +++N+ + G+ + + + + D++ + + ++++ + SN E + ++ N E++ F ++ + + Y HS +FL + + E + F + A+ + ++ ++E S + +++VK L++++ SP++++PR + ++++ +LG I I + ++ SD + +
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSIALLKGAVELENLPLKKDALKEL-ELPFEVKAGFIGKIRIQIPFYRPHLDPWVISISKLHLIGASDKQEGFDELKEKLLEKAHKKALLKALEEKWKSDQERKGES------YWYSVTASIVTRIVDNIELKVQDVHLRFEDGITDPSHPFAFGICIKSVSVQNAVHE-------PVEKSMKKKQLDIADFSVYWDVNTTLLGDLPPGELQDAMDKSM-ESRDHQYILQPICTLALLKRNCSKEPLRSRHQPRIECDICLETIPLILSQVQYQQFVEFVDEFERKERQLKFRKWKPNVPVRKNCKEWWLFALNANLSEIREQRRRSTLDFILHRARDAIYYTDKHFDRIKGIQLTNEEETQLDRIEEEQSFEELKILQELVFER-SKKEHEMAEN-LREPLSDPSA------------SSPNSSLKNSPSSGVLQYLQSWFPGWGGWYGNYQETRSDDFFDPMADSANMNTFTKRDNVFAKLNLKLEGGTVTLLHKEKSSQEAQETPFMQLEFSGNLRA--ESLPRRDSSLLLIQLNGLFLRDLATHGTIFPDLVYPNPVSNWCSSGSE------APVFKMLYEKNPVRS----DFERRLEVSTRPLNIIYNPQAITKVADFFYKGKVHSSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDRLLVGEFIENSRRWTVRLDISAPQVIFPD-DFVSPDPVLVVVDLGRILLTNSEDNL---STEV---------NDFSDEEYKTPLATPPSSPPPEVTSTCETEAPASTKEVLSNDKLEAYLLNNKMYEKYSLSCMDLQIMVGHVKDNWKHMH-----DVEVGPAHVV-EKFNVLLQLQRRLMYTSDPNYPGAILSGTLPDLKIHVNEEKISALQRCFTRLTN--SETKPSDNTLRKEKIFNERENSGRLQDSVMNLTQSIVTLEQHTREVLVES-HLLLAEFKINYMQLGVESCGQYISVLKVFGSNAHFLKRPYDAEVTLTVHGLLMVDTMQTYGSDFDLLMASHKNLSFDAPTGSLRESRAQSPVSPVSEANDFAQPNTGVFKDKYNCSENLKDQESLIKIEYQFVSSECPSMNLDSSLQVMTVQVNNLDIILNPETIVELISFLQKSFPKENVEVSPQPLKSEFESNILEQETFQSTYEQNTHIAVEIHRLNILLFRTVALPVGENCARKIATASIGGTKVNVCL---GSKFDINGSLGCLQLMDLTQDAANSQYVISIGSTSSYDNQASNVEAFECMFDRNLFNSIPMDALSFTFTEHSKEEFNLNLKMASLHYNHSAKFLKELTLSMDELEDNFQNMLKTAASKVSSVLVNKTAEYSEMVSLFETPRKHRDSSQYEEEYDGLNASSPKMDTVKLILNIKIESPVVSIPRSPGNPELLVGHLGQIFIQNFVSDDDLSRSDKLQV 1374
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Xenopus
Match: trim13 (tripartite motif containing 13 [Source:Xenbase;Acc:XB-GENE-961103]) HSP 1 Score: 598.586 bits (1542), Expect = 9.228e-171 Identity = 475/1611 (29.48%), Postives = 759/1611 (47.11%), Query Frame = 2
Query: 6908 FDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICW-------FWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--QERNVAF--------------CDCRK----RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKC-----GDEN---------KWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY----------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQ--YNCNEDQNKP---IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDN--------------LLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPL-----------VYKNWFRPGSGEVLVSVVTDGPTRVMRLID---KEDESILSQSQLDFNADSSTFSNIEMHFSVE-------GFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY------EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
F N KPS N +++ + + E V VC IDDC+D DVPL ++ F+ ++ L N KG + LS DYYN GW P IEPWP ++ W + K + ++ L+ +I+S L+ +T + W ++ QE+ C K + FVPF + N GC + + L+ P + ++ GD+N +W V + F+ ET R+K+R+ +H H++++++ GW PV+VDK+GIF+R+ D + VS+ +R+V + +GS + + IRS L + N L IP+E + ++ + P IPL + +Q RP + F +PI W VN G+ ++ C S + E ++ + C I++ N+P P + PGH I + + N LPC++ F +K + + ++P + + T Q+ V L++F C LLI + ++ V +RLYD RLL L +++ R G + + +S+ YW++N+T LPL++ Q ++T AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG++++ GRGK+ +T MV RYLL N+S++ L F Q + + N P I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITLQ A YR+ FT+ D LP P++++N + + V F Q+ + R + + + + YALDEPTLP I + ++G S ++N + LYY+NF+YIA +F + LV DV P +V L +K RSQLW + L HEGS APH+P K + RS D+ L+LD+ LA + + + ++L K D RR Q W F +G + + G+ + A + ++ G E A L RPGSG + V V+ DGPTRV+++ D + ++ + + + N E+ +E G G+ L+N +PEEL++ + +++ + + L + + +QVDNQ + +++ E + P+ +A + + +L +F + + + +EEK+LLK + F E + E + H +E + + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + + H +ET I I H++ +L SQA KI GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC +S LLP
Sbjct: 2654 FKNAEPLKPSVSNRNNGAASLQWISGVEL----KAESVCVCFIDDCMDCDVPLAELTFSRLSYFQHLVSNPKGFAEFILSGDYYNRELSGWEPFIEPWPCSLSWQQQEASRLHPSRLKLEMKAKQLLDINITSVLIDQYISTKEAWLADYCKQEKECEIDTSEEWMGSSVDPPCLGPKVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPEVVPDGDKNLIDDTHNVSQWREVLPG--QEIPFEFET--REKLRHRH--THDLRIHQLQVRVSGWEPISPVSVDKVGIFFRYAAPDKNTSSSSVSSCISLWSRLYLHFSALPPVRVVFAVTMEGSARKVVTIRSSLMVKNKLEIPMELRLDSPSAPDKPVILPAVMPGESFAIPLHLTSWRLQARP-----------RGMGVFFCKAPIH-------WTNVNKAGEVSSSKRECHSMDA------EISRYFRFCVAIKKDNYPDYVPSNIFSDSAKQIYRQPGHTIFLLPTVVICNLLPCELNFYVK---------GLPIKGTLKPGREMALHTADTSQNFELGVCLENFQHCKELLIPPGTQ-NYMVRMRLYDGHKRLLNLTIRIACRAEGSLKIFVSAPYWLINKTGLPLIFRQDN-SKTDAAGQFEEHELARSLSPLLFCYADKELPNLCTMRIGRGIHPEGI----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGKYRDTCMVTFAPRYLLINKSTYKLAFAQREFARGQGTNNPVGYISTLPGSSVVFHWPRNDYDQLLCVRLMD---LPNCVWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLQSATYRISFTNTDQLPPPFRIDNFSKVPVVFVQH-GVADPRLRTEVKSATSMDYALDEPTLPSYIAVTVKGAGSSEITCNMNDFQDSKQLYYENFIYIAASYTFSGSSEGNVRLGGQNPSITNAELVLDVHPKTLRVVLKKKEPGKRSQLWRMTGTGMLCHEGSSAPHNPNKPQSTRSLDSSLVLDIAGLA---AVTDNKYEPLMLRKPDHRRSTTQTWCFRDGKLTCSLHGL--VVQARNGTSGLYDGAEVVLGPDASLDLLGSVPKEQQFINQKMRPGSGVLSVRVIPDGPTRVLQITDLSQRRSNRFSNEIEELAELELRKYKNPEVKQEIEVLVKLQDGIGISLVNKVPEELVFVSLSGINIHYTHLTVNQVLELSVQNVQVDNQLIGTTQPTMLFVTPSSIESEEEIGPAIQVSAMMVPSKNKLST-----LFKHLLIKAQKFTVQIEEKLLLKLLSYFGFDQSETGV--EKWDENIHDKPSEENKSQQRYYFENLKISIPQIKLSVFTSNKLPPDLKALKSTLGFP-LIKFEDAVIDLDPFAKVHPYETQEFIINDIIKHFQEELLSQAAKILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQTEREYIRYHAATSGEHLV---AGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGFGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGHRMQVQRIRKPRCCTSPQGLLP 4187 HSP 2 Score: 301.597 bits (771), Expect = 1.034e-80 Identity = 338/1474 (22.93%), Postives = 643/1474 (43.62%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFE-KEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAA------LRERARNLVKYVQSYEDYLLN-KDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNA------VNIPAPQQS------------------GWWGYLWGRRSKETSPEI--LDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFT-RDKIFLRGEFSLEGIKLKLL-------KDTEIPILEVN-AKNLIVGFESRPQYSSWCIRIGLRSLFVRDLN------PD--YKNDSFRYVVSSREHSKV---------PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS-LENLSMATDCQASKETKPV------------TNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKL---SRYSSM----------------TIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP-----------------------------------LSESIGD--PIIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQ-----------KPETPNTTEFKEFDVSIACKAVSILLIKISSNLASEN-RFAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTT-------GSNETKMEISENEDVIN-------ISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAIL--SSEESTRIGF--------------------------LNSVK--LHLRMLSPILALPRHKTSNQIILVNLGDIEITSDTQSLQVAFSDAIVI 3802
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+G IG++ ++IP+ R W I I +++I + E KE E KK LL ++E KW+ ++G++ W S AS+ RI+ N++++V++VH+R+ED T PS FA G+ I + S +++ E E + KQ+D++ S+Y D ++ ++ ++ + + + E I+ PI ++KRN + PL HQPRI + +E + + LSQ QY+ V DEFER +RQ K++P V V ++ KEWW+FA+ + I + + RAR+ + Y + D + + +N E + +E + + L L+E+ E +K ++ +N + +P S GW G W +ETS EI L Q Q E P DS G + + D +N FT RD +F + LEG + LL + E P +++ + NL ES P+ S + I L LF+RDL PD Y N + + S + S P +F + +E NP+ S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ +++ + RW V L ISAP++I P+ + +P V++DLGR+ ++N D R + + E +D E++ TP ++P S ++ + +A TK V +Y++YS+ ++ +VG NW + + P ++ E FN +L L+++ + P I+ + L I ++++K+ + C +TN + + +D++ K K +N + + N ++ H+ + +ES LAE + Q + +Y S+ +++ + L++ D GS D ++ASHK L D P SE++ D +I+ Y+ +S + S ++ + ++ ++LD+I N ET+ EL+SF++ F +++++ Q + ET +T + +++ ++ILL + + EN ++ I +++N+ + G+ + + + + D++ + + ++++ + SN E + ++ N E++ F ++ + + Y HS +FL + + E + F + A+ + ++ ++E S + +++VK L++++ SP++++PR + ++++ +LG I I + ++ SD + +
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSIALLKGAVELENLPLKKDALKEL-ELPFEVKAGFIGKIRIQIPFYRPHLDPWVISISKLHLIGASDKQEGFDELKEKLLEKAHKKALLKALEEKWKSDQERKGES------YWYSVTASIVTRIVDNIELKVQDVHLRFEDGITDPSHPFAFGICIKSVSVQNAVHE-------PVEKSMKKKQLDIADFSVYWDVNTTLLGDLPPGELQDAMDKSM-ESRDHQYILQPICTLALLKRNCSKEPLRSRHQPRIECDICLETIPLILSQVQYQQFVEFVDEFERKERQLKFRKWKPNVPVRKNCKEWWLFALNANLSEIREQRRRSTLDFILHRARDAIYYTDKHFDRIKGIQLTNEEETQLDRIEEEQSFEELKILQELVFER-SKKEHEMAENLREPLSDPSASSPNSSLKNSPSSGVLQYLQSWFPGWGG--WYGNYQETSAEIEKLTQEQQQQE---------PWDSENI-MGSDDFFDPM---ADSANMNTFTKRDNVFAKLNLKLEGGTVTLLHKEKSSQEAQETPFMQLEFSGNLRA--ESLPRRDSSLLLIQLNGLFLRDLATHGTIFPDLVYPN-PLKELGSVSQTSNTHIGSTEVLSPKAPVFKMLYEKNPVRS----DFERRLEVSTRPLNIIYNPQAITKVADFFYKGKVHSSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDRLLVGEFIENSRRWTVRLDISAPQVIFPD-DFVSPDPVLVVVDLGRILLTNSEDHYKGRVLDNLSTEV----NDFSDEEYKTPLATPPSSPPPEVTSTCETEAPASTKEVLSNDKLEAYLLNNKMYEKYSLSCMDLQIMVGHVKDNWKHMH-----DVEVGPAHVV-EKFNVLLQLQRRLMYTSDPNYPGAILSGTLPDLKIHVNEEKISALQRCFTRLTN--SETKPSDNTLRKEKIFNERENSGRLQDSVMNLTQSIVTLEQHTREVLVES-HLLLAEFKINYMQLGVESCGQYISVLKVFGSNAHFLKRPYDAEVTLTVHGLLMVDTMQTYGSDFDLLMASHKNLSFDAPTGSLRESRAQSPVSPVSEANDFAQPNTGVFKDKYNCSENLKDQESLIKIEYQFVSSECPSMNLDSSLQVMTVQVNNLDIILNPETIVELISFLQKSFPKENVEVSPQPLKSEFESNILEQETFQSTYEQNTHIAVEIHRLNILLFRTVALPVGENCARKIATASIGGTKVNVCL---GSKFDINGSLGCLQLMDLTQDAANSQYVISIGSTSSYDNQASNVEAFECMFDRNLFNSIPMDALSFTFTEHSKEEFNLNLKMASLHYNHSAKFLKELTLSMDELEDNFQNMLKTAASKVSSVLVNKTAEYSEMVSLFETPRKHRDSSQYEEEYDGLNASSPKMDTVKLILNIKIESPVVSIPRSPGNPELLVGHLGQIFIQNFVSDDDLSRSDKLQV 1419 HSP 3 Score: 172.17 bits (435), Expect = 1.637e-41 Identity = 144/474 (30.38%), Postives = 237/474 (50.00%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILKD-----GDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENT-DHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFP--ANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISF-----QPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
CLLD + +DL +MD+++A R + + DL F +R + L+E+C + L + RNL+ ISH PD I+G LSSV S+ + Y+ IRG+L +NLGE D ++ Y Q DP +HT L+G Y ++ + + +V L L+ +G D N +L R +F +SK +ESFS+ +K+I LV + D R++ A + NVF I+ P K + + Q+ + + S D + F V LN ++ +IFDW+ + + L T E++ H + + KP + S V+ ++ +II E K E+ +NV + F ++E+ S T+A+IL +YK + +R PFS S+ + SC + ++ LSII+PV I I L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 2126 CLLDCIIVDLQDMDIFAAERFSMDFREGFDDFRADLIFPSYFVRQVGGSLLTERCRMKLQLERNLDKEISHAVPDLSIHGNLSSVHCSLDLSDYKLIRGLLENNLGEPVEDFMRPYDLQ----DPKIHTVLSGDVYTSVSFLIDMINVSLELLEE-KGKDGLNN-SLARFDFKKSKFLFESFSNQTKSINLVSHSMMAYDTRFAGQKPLAAHKPNVFNCILQPAKTS----SNPGSLQIELHHRSTKDTSCFTVVLNNLRVFLIFDWLMLVHKF----------LQTPAESKKHVHSSSSEEQKPSNASPVIPKTVKSGVVTKRSSVQIIPE--KQLEVKINVTGTEFVVVEDMSSCDTNAIILKGTTVLTYKPKLADR--PFSGSLFGIEVFSCRVGNELDTALSIIDPVHIQIDLVGN-SAYQNSSGLMDAFNTEDFPPI-LEIQLPALDIRLSYNDVQLFLAIAKSIPKQA 2573 HSP 4 Score: 159.073 bits (401), Expect = 1.541e-37 Identity = 106/306 (34.64%), Postives = 166/306 (54.25%), Query Frame = 2
Query: 4586 NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRF------YDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTK--DGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDE 5479
+ L I + L+DKK FS+ I+R VD DFN L I+++ WV++LDFFG+GS P D + +P + + E NTKL++ + SL+L+N + +N++ L +Y + +G++ L GS+ L DL+ G +Y +R + + L F++FKYGLPD +L RE DMR+ +++ + Y+HTQRF EL F Q+F Q QD L R G+ V AQRS+RI + I AP+LL+P S++S +++ +LGK + N+F F + L D+
Sbjct: 1777 DSLVHINVFLVDKKHPEFSSLYKRINRSVDVDFNCLDILITLQTWVVILDFFGIGSTADNHAVKQQPSDTRQAPKSETNMPLVSEK------EHTNTKLDLKVHSLSLVLKNTRSELAKANVS-------KLTSYLEMIEGDLALQGSIGSLSLSDLTVHGEVYTERF--TTKGEEALIFYIFKYGLPDLALKREYDMRVSLRMA-SVQYVHTQRFQAELVAFIQHFTQLQDVLGRQRAAIQGQTVRNQAQRSSRILLDIEAGAPVLLIPESSWSSKLIVANLGKLRVRNKFLFAGTPETFSLKDK 2066
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Xenopus
Match: trim13 (tripartite motif containing 13 [Source:Xenbase;Acc:XB-GENE-961103]) HSP 1 Score: 596.66 bits (1537), Expect = 3.852e-170 Identity = 472/1614 (29.24%), Postives = 757/1614 (46.90%), Query Frame = 2
Query: 6908 FDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICW-------FWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--QERNVAFCDCRK-------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKC-----GDEN---------KWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQ--YNCNEDQNKP---IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDN--------------LLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPL-----------VYKNWFRPGSGEVLVSVVTDGPTRVMRLID---KEDESILSQSQLDFNADSSTFSNIEMHFSVE-------GFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY------EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
F N KPS N +++ + + E V VC IDDC+D DVPL ++ F+ ++ L N KG + LS DYYN GW P IEPWP ++ W + K + ++ L+ +I+S L+ +T + W ++ QE+ + + FVPF + N GC + + L+ P + ++ GD+N +W V + F+ ET R+K+R+ +H H++++++ GW PV+VDK+GIF+R+ D + V + +R+V + +GS + + IRS L + N L IP+E + ++ + P IPL + +Q RP + F +PI W VN G+ ++ C S + E ++ + C I++ N+P P + PGH I + + N LPC++ F +K + + ++P + + T Q+ V L++F C LLI + ++ V +RLYD RLL L +++ R G + + +S+ YW++N+T LPL++ Q ++T AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG++++ GRGK+ +T MV RYLL N+S++ L F Q + + N P I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITLQ A YR+ FT+ D LP P++++N + + V F Q+ + R + + + + YALDEPTLP I + ++G S ++N + LYY+NF+YIA +F + LV DV P +V L +K RSQLW + L HEGS APH+P K + RS D+ L+LD+ LA + + + ++L K D RR Q W F +G + + G+ + A + ++ G E A L RPGSG + V V+ DGPTRV+++ D + ++ + + + N E+ +E G G+ L+N +PEEL++ + +++ + + L + + +QVDNQ + +++ E + P+ +A + + +L +F + + + +EEK+LLK + F E + E + H +E + + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + + H +ET I I H++ +L SQA KI GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC +S LLP
Sbjct: 2639 FKNAEPLKPSVSNRNNGAASLQWISGVEL----KAESVCVCFIDDCMDCDVPLAELTFSRLSYFQHLVSNPKGFAEFILSGDYYNRELSGWEPFIEPWPCSLSWQQQEASRLHPSRLKLEMKAKQLLDINITSVLIDQYISTKEAWLADYCKQEKECEIDTSEEWMGSSVDPPCLGPTEVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPEVVPDGDKNLIDDTHNVSQWREVLPG--QEIPFEFET--REKLRHRH--THDLRIHQLQVRVSGWEPISPVSVDKVGIFFRYAAPDKNTSSSSVGSPISKTNIIHPQVYFSALPPVRVVFAVTMEGSARKVVTIRSSLMVKNKLEIPMELRLDSPSAPDKPVILPAVMPGESFAIPLHLTSWRLQARP-----------RGMGVFFCKAPIH-------WTNVNKAGEVSSSKRECHSMDA------EISRYFRFCVAIKKDNYPDYVPSNIFSDSAKQIYRQPGHTIFLLPTVVICNLLPCELNFYVK---------GLPIKGTLKPGREMALHTADTSQNFELGVCLENFQHCKELLIPPGTQ-NYMVRMRLYDGHKRLLNLTIRIACRAEGSLKIFVSAPYWLINKTGLPLIFRQDN-SKTDAAGQFEEHELARSLSPLLFCYADKELPNLCTMRIGRGIHPEGI----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGKYRDTCMVTFAPRYLLINKSTYKLAFAQREFARGQGTNNPVGYISTLPGSSVVFHWPRNDYDQLLCVRLMD---LPNCVWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLQSATYRISFTNTDQLPPPFRIDNFSKVPVVFVQH-GVADPRLRTEVKSATSMDYALDEPTLPSYIAVTVKGAGSSEITCNMNDFQDSKQLYYENFIYIAASYTFSGSSEGNVRLGGQNPSITNAELVLDVHPKTLRVVLKKKEPGKRSQLWRMTGTGMLCHEGSSAPHNPNKPQSTRSLDSSLVLDIAGLA---AVTDNKYEPLMLRKPDHRRSTTQTWCFRDGKLTCSLHGL--VVQARNGTSGLYDGAEVVLGPDASLDLLGSVPKEQQFINQKMRPGSGVLSVRVIPDGPTRVLQITDLSQRRSNRFSNEIEELAELELRKYKNPEVKQEIEVLVKLQDGIGISLVNKVPEELVFVSLSGINIHYTHLTVNQVLELSVQNVQVDNQLIGTTQPTMLFVTPSSIESEEEIGPAIQVSAMMVPSKNKLST-----LFKHLLIKAQKFTVQIEEKLLLKLLSYFGFDQSETGV--EKWDENIHDKPSEENKSQQRYYFENLKISIPQIKLSVFTSNKLPPDLKALKSTLGFP-LIKFEDAVIDLDPFAKVHPYETQEFIINDIIKHFQEELLSQAAKILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQTEREYIRYHAATSGEHLV---AGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGFGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGHRMQVQRIRKPRCCTSPQGLLP 4175 HSP 2 Score: 300.442 bits (768), Expect = 2.686e-80 Identity = 248/824 (30.10%), Postives = 400/824 (48.54%), Query Frame = 2
Query: 4586 NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRF------YDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTK--DGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENN-------------------------------------------------DRSRCLLDEMKIDLHEMDVYSAIRITDSILKD-----GDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENT-DHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFP--ANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISF-----QPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
+ L I + L+DKK FS+ I+R VD DFN L I+++ WV++LDFFG+GS P D + +P + + E NTKL++ + SL+L+N + +N++ L +Y + +G++ L GS+ L DL+ G +Y +R + + L F++FKYGLPD +L RE DMR+ +++ + Y+HTQRF EL F Q+F Q QD L R G+ V AQRS+RI + I AP+LL+P S++S +++ +LGK + N+F F + CLLD + +DL +MD+++A R + + DL F +R + L+E+C + L + RNL+ ISH PD I+G LSSV S+ + Y+ IRG+L +NLGE D ++ Y Q DP +HT L+G Y ++ + + +V L L+ +G D N +L R +F +SK +ESFS+ +K+I LV + D R++ A + NVF I+ P K + + Q+ + + S D + F V LN ++ +IFDW+ + + L T E++ H + + KP + S V+ ++ +II E K E+ +NV + F ++E+ S T+A+IL +YK + +R PFS S+ + SC + ++ LSII+PV I I L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 1777 DSLVHINVFLVDKKHPEFSSLYKRINRSVDVDFNCLDILITLQTWVVILDFFGIGSTADNHAVKQQPSDTRQAPKSETNMPLVSEK------EHTNTKLDLKVHSLSLVLKNTRSELAKANVS-------KLTSYLEMIEGDLALQGSIGSLSLSDLTVHGEVYTERF--TTKGEEALIFYIFKYGLPDLALKREYDMRVSLRMA-SVQYVHTQRFQAELVAFIQHFTQLQDVLGRQRAAIQGQTVRNQAQRSSRILLDIEAGAPVLLIPESSWSSKLIVANLGKLRVRNKFLFAGTPETFSLKDKVYFTYVWLHCMIFNEYIYFFFEPLAMVMYSNINIFYSSFTEEHICLLDCIIVDLQDMDIFAAERFSMDFREGFDDFRADLIFPSYFVRQVGGSLLTERCRMKLQLERNLDKEISHAVPDLSIHGNLSSVHCSLDLSDYKLIRGLLENNLGEPVEDFMRPYDLQ----DPKIHTVLSGDVYTSVSFLIDMINVSLELLEE-KGKDGLNN-SLARFDFKKSKFLFESFSNQTKSINLVSHSMMAYDTRFAGQKPLAAHKPNVFNCILQPAKTS----SNPGSLQIELHHRSTKDTSCFTVVLNNLRVFLIFDWLMLVHKF----------LQTPAESKKHVHSSSSEEQKPSNASPVIPKTVKSGVVTKRSSVQIIPE--KQLEVKINVTGTEFVVVEDMSSCDTNAIILKGTTVLTYKPKLADR--PFSGSLFGIEVFSCRVGNELDTALSIIDPVHIQIDLVGN-SAYQNSSGLMDAFNTEDFPPI-LEIQLPALDIRLSYNDVQLFLAIAKSIPKQA 2558 HSP 3 Score: 299.286 bits (765), Expect = 5.255e-80 Identity = 337/1474 (22.86%), Postives = 643/1474 (43.62%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFE-KEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAA------LRERARNLVKYVQSYEDYLLN-KDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNA------VNIPAPQQS------------------GWWGYLWGRRSKETSPEI--LDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFT-RDKIFLRGEFSLEGIKLKLL-------KDTEIPILEVN-AKNLIVGFESRPQYSSWCIRIGLRSLFVRDLN------PD--YKNDSFRYVVSSREHSKV---------PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS-LENLSMATDCQASKETKPV------------TNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKL---SRYSSM----------------TIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP-----------------------------------LSESIGD--PIIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQ-----------KPETPNTTEFKEFDVSIACKAVSILLIKISSNLASEN-RFAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTT-------GSNETKMEISENEDVIN-------ISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAIL--SSEESTRIGF--------------------------LNSVK--LHLRMLSPILALPRHKTSNQIILVNLGDIEITSDTQSLQVAFSDAIVI 3802
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+G IG++ ++IP+ R W I I +++I + E KE E KK LL ++E KW+ ++G++ W S AS+ RI+ N++++V++VH+R+ED T PS FA G+ I + S +++ E E + KQ+D++ S+Y D ++ ++ ++ + + + E I+ PI ++KRN + PL HQPRI + +E + + LSQ QY+ V DEFER +RQ K++P V V ++ KEWW+FA+ + I + + RAR+ + Y + D + + +N E + +E + + L L+E+ E +K ++ +N + +P S GW G W +ETS EI L Q Q E P DS G + + D +N FT RD +F + LEG + LL + E P +++ + NL ES P+ S + I L LF+RDL PD Y N + + S + S P +F + +E NP+ S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ +++ + RW V L ISAP++I P+ + +P V++DLGR+ ++N D + + + E +D E++ TP ++P S ++ + +A TK V +Y++YS+ ++ +VG NW + + P ++ E FN +L L+++ + P I+ + L I ++++K+ + C +TN + + +D++ K K +N + + N ++ H+ + +ES LAE + Q + +Y S+ +++ + L++ D GS D ++ASHK L D P SE++ D +I+ Y+ +S + S ++ + ++ ++LD+I N ET+ EL+SF++ F +++++ Q + ET +T + +++ ++ILL + + EN ++ I +++N+ + G+ + + + + D++ + + ++++ + SN E + ++ N E++ F ++ + + Y HS +FL + + E + F + A+ + ++ ++E S + +++VK L++++ SP++++PR + ++++ +LG I I + ++ SD + +
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSIALLKGAVELENLPLKKDALKEL-ELPFEVKAGFIGKIRIQIPFYRPHLDPWVISISKLHLIGASDKQEGFDELKEKLLEKAHKKALLKALEEKWKSDQERKGES------YWYSVTASIVTRIVDNIELKVQDVHLRFEDGITDPSHPFAFGICIKSVSVQNAVHE-------PVEKSMKKKQLDIADFSVYWDVNTTLLGDLPPGELQDAMDKSM-ESRDHQYILQPICTLALLKRNCSKEPLRSRHQPRIECDICLETIPLILSQVQYQQFVEFVDEFERKERQLKFRKWKPNVPVRKNCKEWWLFALNANLSEIREQRRRSTLDFILHRARDAIYYTDKHFDRIKGIQLTNEEETQLDRIEEEQSFEELKILQELVFER-SKKEHEMAENLREPLSDPSASSPNSSLKNSPSSGVLQYLQSWFPGWGG--WYGNYQETSAEIEKLTQEQQQQE---------PWDSENI-MGSDDFFDPM---ADSANMNTFTKRDNVFAKLNLKLEGGTVTLLHKEKSSQEAQETPFMQLEFSGNLRA--ESLPRRDSSLLLIQLNGLFLRDLATHGTIFPDLVYPN-PLKELGSVSQTSNTHIGSTEVLSPKAPVFKMLYEKNPVRS----DFERRLEVSTRPLNIIYNPQAITKVADFFYKGKVHSSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDRLLVGEFIENSRRWTVRLDISAPQVIFPD-DFVSPDPVLVVVDLGRILLTNSEDNSFIKVLDNLSTEV----NDFSDEEYKTPLATPPSSPPPEVTSTCETEAPASTKEVLSNDKLEAYLLNNKMYEKYSLSCMDLQIMVGHVKDNWKHMH-----DVEVGPAHVV-EKFNVLLQLQRRLMYTSDPNYPGAILSGTLPDLKIHVNEEKISALQRCFTRLTN--SETKPSDNTLRKEKIFNERENSGRLQDSVMNLTQSIVTLEQHTREVLVES-HLLLAEFKINYMQLGVESCGQYISVLKVFGSNAHFLKRPYDAEVTLTVHGLLMVDTMQTYGSDFDLLMASHKNLSFDAPTGSLRESRAQSPVSPVSEANDFAQPNTGVFKDKYNCSENLKDQESLIKIEYQFVSSECPSMNLDSSLQVMTVQVNNLDIILNPETIVELISFLQKSFPKENVEVSPQPLKSEFESNILEQETFQSTYEQNTHIAVEIHRLNILLFRTVALPVGENCARKIATASIGGTKVNVCL---GSKFDINGSLGCLQLMDLTQDAANSQYVISIGSTSSYDNQASNVEAFECMFDRNLFNSIPMDALSFTFTEHSKEEFNLNLKMASLHYNHSAKFLKELTLSMDELEDNFQNMLKTAASKVSSVLVNKTAEYSEMVSLFETPRKHRDSSQYEEEYDGLNASSPKMDTVKLILNIKIESPVVSIPRSPGNPELLVGHLGQIFIQNFVSDDDLSRSDKLQV 1419
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Xenopus
Match: trim13 (tripartite motif containing 13 [Source:Xenbase;Acc:XB-GENE-961103]) HSP 1 Score: 595.89 bits (1535), Expect = 7.123e-170 Identity = 470/1589 (29.58%), Postives = 750/1589 (47.20%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICW-------FWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--QE------------------------RNVAFCDCRK--------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKC-----GDEN---------KWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY---IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQ--YNCNEDQNKP---IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDN--------------LLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPL-----------VYKNWFRPGSGEVLVSVVTDGPTRVMRLID---KEDESILSQSQLDFNADSSTFSNIEMHFSVE-------GFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY------EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V VC IDDC+D DVPL ++ F+ ++ L N KG + LS DYYN GW P IEPWP ++ W + K + ++ L+ +I+S L+ +T + W ++ QE R +A+ R + FVPF + N GC + + L+ P + ++ GD+N +W V + F+ ET R+K+R+ +H H++++++ GW PV+VDK+GIF+R+ D + VS+ +R+V + +GS + + IRS L + N L IP+E + ++ + P IPL + +Q RP + F +PI W VN G+ ++ C S + E ++ + C I++ N+P P + PGH I + + N LPC++ F +K + + ++P + + T Q+ V L++F C LLI + ++ V +RLYD RLL L +++ R G + + +S+ YW++N+T LPL++ Q ++T AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG++++ GRGK+ +T MV RYLL N+S++ L F Q + + N P I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITLQ A YR+ FT+ D LP P++++N + + V F Q+ + R + + + + YALDEPTLP I + ++G S ++N + LYY+NF+YIA +F + LV DV P +V L +K RSQLW + L HEGS APH+P K + RS D+ L+LD+ LA + + + ++L K D RR Q W F +G + + G+ + A + ++ G E A L RPGSG + V V+ DGPTRV+++ D + ++ + + + N E+ +E G G+ L+N +PEEL++ + +++ + + L + + +QVDNQ + +++ E + P+ +A + + +L +F + + + +EEK+LLK + F E + E + H +E + + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + + H +ET I I H++ +L SQA KI GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC +S LLP
Sbjct: 2677 ESVCVCFIDDCMDCDVPLAELTFSRLSYFQHLVSNPKGFAEFILSGDYYNRELSGWEPFIEPWPCSLSWQQQEASRLHPSRLKLEMKAKQLLDINITSVLIDQYISTKEAWLADYCKQEKECEIDTSEEWMGSSVDPPCLGPSRPLAYLRTRSTASLTNLEHQVYARAEVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPEVVPDGDKNLIDDTHNVSQWREVLPG--QEIPFEFET--REKLRHRH--THDLRIHQLQVRVSGWEPISPVSVDKVGIFFRYAAPDKNTSSSSVSSSLPPVRVVFAVTMEGSARKVVTIRSSLMVKNKLEIPMELRLDSPSAPDKPVILPAVMPGESFAIPLHLTSWRLQARP-----------RGMGVFFCKAPIH-------WTNVNKAGEVSSSKRECHSMDA------EISRYFRFCVAIKKDNYPDYVPSNIFSDSAKQIYRQPGHTIFLLPTVVICNLLPCELNFYVK---------GLPIKGTLKPGREMALHTADTSQNFELGVCLENFQHCKELLIPPGTQ-NYMVRMRLYDGHKRLLNLTIRIACRAEGSLKIFVSAPYWLINKTGLPLIFRQDN-SKTDAAGQFEEHELARSLSPLLFCYADKELPNLCTMRIGRGIHPEGI----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGKYRDTCMVTFAPRYLLINKSTYKLAFAQREFARGQGTNNPVGYISTLPGSSVVFHWPRNDYDQLLCVRLMD---LPNCVWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLQSATYRISFTNTDQLPPPFRIDNFSKVPVVFVQH-GVADPRLRTEVKSATSMDYALDEPTLPSYIAVTVKGAGSSEITCNMNDFQDSKQLYYENFIYIAASYTFSGSSEGNVRLGGQNPSITNAELVLDVHPKTLRVVLKKKEPGKRSQLWRMTGTGMLCHEGSSAPHNPNKPQSTRSLDSSLVLDIAGLA---AVTDNKYEPLMLRKPDHRRSTTQTWCFRDGKLTCSLHGL--VVQARNGTSGLYDGAEVVLGPDASLDLLGSVPKEQQFINQKMRPGSGVLSVRVIPDGPTRVLQITDLSQRRSNRFSNEIEELAELELRKYKNPEVKQEIEVLVKLQDGIGISLVNKVPEELVFVSLSGINIHYTHLTVNQVLELSVQNVQVDNQLIGTTQPTMLFVTPSSIESEEEIGPAIQVSAMMVPSKNKLST-----LFKHLLIKAQKFTVQIEEKLLLKLLSYFGFDQSETGV--EKWDENIHDKPSEENKSQQRYYFENLKISIPQIKLSVFTSNKLPPDLKALKSTLGFP-LIKFEDAVIDLDPFAKVHPYETQEFIINDIIKHFQEELLSQAAKILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQTEREYIRYHAATSGEHLV---AGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGFGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGHRMQVQRIRKPRCCTSPQGLLP 4192 HSP 2 Score: 300.442 bits (768), Expect = 2.373e-80 Identity = 340/1478 (23.00%), Postives = 647/1478 (43.78%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFE-KEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAA------LRERARNLVKYVQSYEDYLLN-KDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNA------VNIPAPQQS------------------GWWGYLWGRRSKETSPEI--LDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFT-RDKIFLRGEFSLEGIKLKLL-------KDTEIPILEVN-AKNLIVGFESRPQYSSWCIRIGLRSLFVRDLN------PD--YKN----------DSFRYVVSSREHSKVPGGE--IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDR-SIKVTAPEPMNYESDLESEDFVTPCSSPFGS-LENLSMATDCQASKETKPV------------TNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKL---SRYSSM----------------TIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP-----------------------------------LSESIGD--PIIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQ-----------KPETPNTTEFKEFDVSIACKAVSILLIKISSNLASEN-RFAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTT-------GSNETKMEISENEDVIN-------ISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAIL--SSEESTRIGF--------------------------LNSVK--LHLRMLSPILALPRHKTSNQIILVNLGDIEITSDTQSLQVAFSDAIVI 3802
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+G IG++ ++IP+ R W I I +++I + E KE E KK LL ++E KW+ ++G++ W S AS+ RI+ N++++V++VH+R+ED T PS FA G+ I + S +++ E E + KQ+D++ S+Y D ++ ++ ++ + + + E I+ PI ++KRN + PL HQPRI + +E + + LSQ QY+ V DEFER +RQ K++P V V ++ KEWW+FA+ + I + + RAR+ + Y + D + + +N E + +E + + L L+E+ E +K ++ +N + +P S GW G W +ETS EI L Q Q E P DS G + + D +N FT RD +F + LEG + LL + E P +++ + NL ES P+ S + I L LF+RDL PD Y N S ++ S+ S G E +F + +E NP+ S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ +++ + RW V L ISAP++I P+ + +P V++DLGR+ ++N +++D S +V +D E++ TP ++P S ++ + +A TK V +Y++YS+ ++ +VG NW + + P ++ E FN +L L+++ + P I+ + L I ++++K+ + C +TN + + +D++ K K +N + + N ++ H+ + +ES LAE + Q + +Y S+ +++ + L++ D GS D ++ASHK L D P SE++ D +I+ Y+ +S + S ++ + ++ ++LD+I N ET+ EL+SF++ F +++++ Q + ET +T + +++ ++ILL + + EN ++ I +++N+ + G+ + + + + D++ + + ++++ + SN E + ++ N E++ F ++ + + Y HS +FL + + E + F + A+ + ++ ++E S + +++VK L++++ SP++++PR + ++++ +LG I I + ++ SD + +
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSIALLKGAVELENLPLKKDALKEL-ELPFEVKAGFIGKIRIQIPFYRPHLDPWVISISKLHLIGASDKQEGFDELKEKLLEKAHKKALLKALEEKWKSDQERKGES------YWYSVTASIVTRIVDNIELKVQDVHLRFEDGITDPSHPFAFGICIKSVSVQNAVHE-------PVEKSMKKKQLDIADFSVYWDVNTTLLGDLPPGELQDAMDKSM-ESRDHQYILQPICTLALLKRNCSKEPLRSRHQPRIECDICLETIPLILSQVQYQQFVEFVDEFERKERQLKFRKWKPNVPVRKNCKEWWLFALNANLSEIREQRRRSTLDFILHRARDAIYYTDKHFDRIKGIQLTNEEETQLDRIEEEQSFEELKILQELVFER-SKKEHEMAENLREPLSDPSASSPNSSLKNSPSSGVLQYLQSWFPGWGG--WYGNYQETSAEIEKLTQEQQQQE---------PWDSENI-MGSDDFFDPM---ADSANMNTFTKRDNVFAKLNLKLEGGTVTLLHKEKSSQEAQETPFMQLEFSGNLRA--ESLPRRDSSLLLIQLNGLFLRDLATHGTIFPDLVYPNPLKELGSVSQTSNTHIGSTEVLSPTSGSEAPVFKMLYEKNPVRS----DFERRLEVSTRPLNIIYNPQAITKVADFFYKGKVHSSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDRLLVGEFIENSRRWTVRLDISAPQVIFPD-DFVSPDPVLVVVDLGRILLTNSEGRVLDNLSTEV---------NDFSDEEYKTPLATPPSSPPPEVTSTCETEAPASTKEVLSNDKLEAYLLNNKMYEKYSLSCMDLQIMVGHVKDNWKHMH-----DVEVGPAHVV-EKFNVLLQLQRRLMYTSDPNYPGAILSGTLPDLKIHVNEEKISALQRCFTRLTN--SETKPSDNTLRKEKIFNERENSGRLQDSVMNLTQSIVTLEQHTREVLVES-HLLLAEFKINYMQLGVESCGQYISVLKVFGSNAHFLKRPYDAEVTLTVHGLLMVDTMQTYGSDFDLLMASHKNLSFDAPTGSLRESRAQSPVSPVSEANDFAQPNTGVFKDKYNCSENLKDQESLIKIEYQFVSSECPSMNLDSSLQVMTVQVNNLDIILNPETIVELISFLQKSFPKENVEVSPQPLKSEFESNILEQETFQSTYEQNTHIAVEIHRLNILLFRTVALPVGENCARKIATASIGGTKVNVCL---GSKFDINGSLGCLQLMDLTQDAANSQYVISIGSTSSYDNQASNVEAFECMFDRNLFNSIPMDALSFTFTEHSKEEFNLNLKMASLHYNHSAKFLKELTLSMDELEDNFQNMLKTAASKVSSVLVNKTAEYSEMVSLFETPRKHRDSSQYEEEYDGLNASSPKMDTVKLILNIKIESPVVSIPRSPGNPELLVGHLGQIFIQNFVSDDDLSRSDKLQV 1419 HSP 3 Score: 287.345 bits (734), Expect = 2.369e-76 Identity = 250/831 (30.08%), Postives = 398/831 (47.89%), Query Frame = 2
Query: 4586 NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRF------YDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTK--DGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDEMKID---LH----------------------EMDVYSAIRI------------TDSILKD------------------------GDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENT-DHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFP--ANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISF-----QPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
+ L I + L+DKK FS+ I+R VD DFN L I+++ WV++LDFFG+GS P D + +P + + E NTKL++ + SL+L+N + +N++ L +Y + +G++ L GS+ L DL+ G +Y +R + + L F++FKYGLPD +L RE DMR+ +++ + Y+HTQRF EL F Q+F Q QD L R G+ V AQRS+RI + I AP+LL+P S++S +++ +LGK + N+F F + L D++ LH M +YS I I D I+ D DL F +R + L+E+C + L + RNL+ ISH PD I+G LSSV S+ + Y+ IRG+L +NLGE D ++ Y Q DP +HT L+G Y ++ + + +V L L+ +G D N +L R +F +SK +ESFS+ +K+I LV + D R++ A + NVF I+ P K + + Q+ + + S D + F V LN ++ +IFDW+ + + L T E++ H + + KP + S V+ ++ +II E K E+ +NV + F ++E+ S T+A+IL +YK + +R PFS S+ + SC + ++ LSII+PV I I L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 1777 DSLVHINVFLVDKKHPEFSSLYKRINRSVDVDFNCLDILITLQTWVVILDFFGIGSTADNHAVKQQPSDTRQAPKSETNMPLVSEK------EHTNTKLDLKVHSLSLVLKNTRSELAKANVS-------KLTSYLEMIEGDLALQGSIGSLSLSDLTVHGEVYTERF--TTKGEEALIFYIFKYGLPDLALKREYDMRVSLRMA-SVQYVHTQRFQAELVAFIQHFTQLQDVLGRQRAAIQGQTVRNQAQRSSRILLDIEAGAPVLLIPESSWSSKLIVANLGKLRVRNKFLFAGTPETFSLKDKVYFTYVWLHLISTPDTLGPSLMDDLNFKEPLAMVMYSNINIFYSSFTEEHICLLDCIIVDLQDMDIFAAERFSMDFREGFDDFRADLIFPSYFVRQVGGSLLTERCRMKLQLERNLDKEISHAVPDLSIHGNLSSVHCSLDLSDYKLIRGLLENNLGEPVEDFMRPYDLQ----DPKIHTVLSGDVYTSVSFLIDMINVSLELLEE-KGKDGLNN-SLARFDFKKSKFLFESFSNQTKSINLVSHSMMAYDTRFAGQKPLAAHKPNVFNCILQPAKTS----SNPGSLQIELHHRSTKDTSCFTVVLNNLRVFLIFDWLMLVHKF----------LQTPAESKKHVHSSSSEEQKPSNASPVIPKTVKSGVVTKRSSVQIIPE--KQLEVKINVTGTEFVVVEDMSSCDTNAIILKGTTVLTYKPKLADR--PFSGSLFGIEVFSCRVGNELDTALSIIDPVHIQIDLVGN-SAYQNSSGLMDAFNTEDFPPI-LEIQLPALDIRLSYNDVQLFLAIAKSIPKQA 2565
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Xenopus
Match: trim13 (tripartite motif containing 13 [Source:Xenbase;Acc:XB-GENE-961103]) HSP 1 Score: 592.038 bits (1525), Expect = 1.077e-168 Identity = 476/1636 (29.10%), Postives = 761/1636 (46.52%), Query Frame = 2
Query: 6908 FDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICW-------FWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--QE------------------------RNVAFCDCRK-------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKC-----GDEN---------KWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY----------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQ--YNCNEDQNKP---IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDN--------------LLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPL-----------VYKNWFRPGSGEVLVSVVTDGPTRVMRLID---KEDESILSQSQLDFNADSSTFSNIEMHFSVE-------GFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY------EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
F N KPS N +++ + + E V VC IDDC+D DVPL ++ F+ ++ L N KG + LS DYYN GW P IEPWP ++ W + K + ++ L+ +I+S L+ +T + W ++ QE R +A+ R + FVPF + N GC + + L+ P + ++ GD+N +W V + F+ ET R+K+R+ +H H++++++ GW PV+VDK+GIF+R+ D + VS+ +R+V + +GS + + IRS L + N L IP+E + ++ + P IPL + +Q RP + F +PI W VN G+ ++ C S + E ++ + C I++ N+P P + PGH I + + N LPC++ F +K + + ++P + + T Q+ V L++F C LLI + ++ V +RLYD RLL L +++ R G + + +S+ YW++N+T LPL++ Q ++T AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG++++ GRGK+ +T MV RYLL N+S++ L F Q + + N P I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITLQ A YR+ FT+ D LP P++++N + + V F Q+ + R + + + + YALDEPTLP I + ++G S ++N + LYY+NF+YIA +F + LV DV P +V L +K RSQLW + L HEGS APH+P K + RS D+ L+LD+ LA + + + ++L K D RR Q W F +G + + G+ + A + ++ G E A L RPGSG + V V+ DGPTRV+++ D + ++ + + + N E+ +E G G+ L+N +PEEL++ + +++ + + L + + +QVDNQ + +++ E + P+ +A + + +L +F + + + +EEK+LLK + F E + E + H +E + + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + + H +ET I I H++ +L SQA KI GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC +S LLP
Sbjct: 2654 FKNAEPLKPSVSNRNNGAASLQWISGVEL----KAESVCVCFIDDCMDCDVPLAELTFSRLSYFQHLVSNPKGFAEFILSGDYYNRELSGWEPFIEPWPCSLSWQQQEASRLHPSRLKLEMKAKQLLDINITSVLIDQYISTKEAWLADYCKQEKECEIDTSEEWMGSSVDPPCLGPSRPLAYLRTRSTASLTNLEHQVYAREVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPEVVPDGDKNLIDDTHNVSQWREVLPG--QEIPFEFET--REKLRHRH--THDLRIHQLQVRVSGWEPISPVSVDKVGIFFRYAAPDKNTSSSSVSSCISLWSRLYLHFSALPPVRVVFAVTMEGSARKVVTIRSSLMVKNKLEIPMELRLDSPSAPDKPVILPAVMPGESFAIPLHLTSWRLQARP-----------RGMGVFFCKAPIH-------WTNVNKAGEVSSSKRECHSMDA------EISRYFRFCVAIKKDNYPDYVPSNIFSDSAKQIYRQPGHTIFLLPTVVICNLLPCELNFYVK---------GLPIKGTLKPGREMALHTADTSQNFELGVCLENFQHCKELLIPPGTQ-NYMVRMRLYDGHKRLLNLTIRIACRAEGSLKIFVSAPYWLINKTGLPLIFRQDN-SKTDAAGQFEEHELARSLSPLLFCYADKELPNLCTMRIGRGIHPEGI----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGKYRDTCMVTFAPRYLLINKSTYKLAFAQREFARGQGTNNPVGYISTLPGSSVVFHWPRNDYDQLLCVRLMD---LPNCVWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLQSATYRISFTNTDQLPPPFRIDNFSKVPVVFVQH-GVADPRLRTEVKSATSMDYALDEPTLPSYIAVTVKGAGSSEITCNMNDFQDSKQLYYENFIYIAASYTFSGSSEGNVRLGGQNPSITNAELVLDVHPKTLRVVLKKKEPGKRSQLWRMTGTGMLCHEGSSAPHNPNKPQSTRSLDSSLVLDIAGLA---AVTDNKYEPLMLRKPDHRRSTTQTWCFRDGKLTCSLHGL--VVQARNGTSGLYDGAEVVLGPDASLDLLGSVPKEQQFINQKMRPGSGVLSVRVIPDGPTRVLQITDLSQRRSNRFSNEIEELAELELRKYKNPEVKQEIEVLVKLQDGIGISLVNKVPEELVFVSLSGINIHYTHLTVNQVLELSVQNVQVDNQLIGTTQPTMLFVTPSSIESEEEIGPAIQVSAMMVPSKNKLST-----LFKHLLIKAQKFTVQIEEKLLLKLLSYFGFDQSETGV--EKWDENIHDKPSEENKSQQRYYFENLKISIPQIKLSVFTSNKLPPDLKALKSTLGFP-LIKFEDAVIDLDPFAKVHPYETQEFIINDIIKHFQEELLSQAAKILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQTEREYIRYHAATSGEHLV---AGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGFGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGHRMQVQRIRKPRCCTSPQGLLP 4212 HSP 2 Score: 301.597 bits (771), Expect = 1.156e-80 Identity = 338/1474 (22.93%), Postives = 643/1474 (43.62%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFE-KEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAA------LRERARNLVKYVQSYEDYLLN-KDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNA------VNIPAPQQS------------------GWWGYLWGRRSKETSPEI--LDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFT-RDKIFLRGEFSLEGIKLKLL-------KDTEIPILEVN-AKNLIVGFESRPQYSSWCIRIGLRSLFVRDLN------PD--YKNDSFRYVVSSREHSKV---------PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS-LENLSMATDCQASKETKPV------------TNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKL---SRYSSM----------------TIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP-----------------------------------LSESIGD--PIIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQ-----------KPETPNTTEFKEFDVSIACKAVSILLIKISSNLASEN-RFAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTT-------GSNETKMEISENEDVIN-------ISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAIL--SSEESTRIGF--------------------------LNSVK--LHLRMLSPILALPRHKTSNQIILVNLGDIEITSDTQSLQVAFSDAIVI 3802
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+G IG++ ++IP+ R W I I +++I + E KE E KK LL ++E KW+ ++G++ W S AS+ RI+ N++++V++VH+R+ED T PS FA G+ I + S +++ E E + KQ+D++ S+Y D ++ ++ ++ + + + E I+ PI ++KRN + PL HQPRI + +E + + LSQ QY+ V DEFER +RQ K++P V V ++ KEWW+FA+ + I + + RAR+ + Y + D + + +N E + +E + + L L+E+ E +K ++ +N + +P S GW G W +ETS EI L Q Q E P DS G + + D +N FT RD +F + LEG + LL + E P +++ + NL ES P+ S + I L LF+RDL PD Y N + + S + S P +F + +E NP+ S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ +++ + RW V L ISAP++I P+ + +P V++DLGR+ ++N D R + + E +D E++ TP ++P S ++ + +A TK V +Y++YS+ ++ +VG NW + + P ++ E FN +L L+++ + P I+ + L I ++++K+ + C +TN + + +D++ K K +N + + N ++ H+ + +ES LAE + Q + +Y S+ +++ + L++ D GS D ++ASHK L D P SE++ D +I+ Y+ +S + S ++ + ++ ++LD+I N ET+ EL+SF++ F +++++ Q + ET +T + +++ ++ILL + + EN ++ I +++N+ + G+ + + + + D++ + + ++++ + SN E + ++ N E++ F ++ + + Y HS +FL + + E + F + A+ + ++ ++E S + +++VK L++++ SP++++PR + ++++ +LG I I + ++ SD + +
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSIALLKGAVELENLPLKKDALKEL-ELPFEVKAGFIGKIRIQIPFYRPHLDPWVISISKLHLIGASDKQEGFDELKEKLLEKAHKKALLKALEEKWKSDQERKGES------YWYSVTASIVTRIVDNIELKVQDVHLRFEDGITDPSHPFAFGICIKSVSVQNAVHE-------PVEKSMKKKQLDIADFSVYWDVNTTLLGDLPPGELQDAMDKSM-ESRDHQYILQPICTLALLKRNCSKEPLRSRHQPRIECDICLETIPLILSQVQYQQFVEFVDEFERKERQLKFRKWKPNVPVRKNCKEWWLFALNANLSEIREQRRRSTLDFILHRARDAIYYTDKHFDRIKGIQLTNEEETQLDRIEEEQSFEELKILQELVFER-SKKEHEMAENLREPLSDPSASSPNSSLKNSPSSGVLQYLQSWFPGWGG--WYGNYQETSAEIEKLTQEQQQQE---------PWDSENI-MGSDDFFDPM---ADSANMNTFTKRDNVFAKLNLKLEGGTVTLLHKEKSSQEAQETPFMQLEFSGNLRA--ESLPRRDSSLLLIQLNGLFLRDLATHGTIFPDLVYPN-PLKELGSVSQTSNTHIGSTEVLSPKAPVFKMLYEKNPVRS----DFERRLEVSTRPLNIIYNPQAITKVADFFYKGKVHSSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDRLLVGEFIENSRRWTVRLDISAPQVIFPD-DFVSPDPVLVVVDLGRILLTNSEDHYKGRVLDNLSTEV----NDFSDEEYKTPLATPPSSPPPEVTSTCETEAPASTKEVLSNDKLEAYLLNNKMYEKYSLSCMDLQIMVGHVKDNWKHMH-----DVEVGPAHVV-EKFNVLLQLQRRLMYTSDPNYPGAILSGTLPDLKIHVNEEKISALQRCFTRLTN--SETKPSDNTLRKEKIFNERENSGRLQDSVMNLTQSIVTLEQHTREVLVES-HLLLAEFKINYMQLGVESCGQYISVLKVFGSNAHFLKRPYDAEVTLTVHGLLMVDTMQTYGSDFDLLMASHKNLSFDAPTGSLRESRAQSPVSPVSEANDFAQPNTGVFKDKYNCSENLKDQESLIKIEYQFVSSECPSMNLDSSLQVMTVQVNNLDIILNPETIVELISFLQKSFPKENVEVSPQPLKSEFESNILEQETFQSTYEQNTHIAVEIHRLNILLFRTVALPVGENCARKIATASIGGTKVNVCL---GSKFDINGSLGCLQLMDLTQDAANSQYVISIGSTSSYDNQASNVEAFECMFDRNLFNSIPMDALSFTFTEHSKEEFNLNLKMASLHYNHSAKFLKELTLSMDELEDNFQNMLKTAASKVSSVLVNKTAEYSEMVSLFETPRKHRDSSQYEEEYDGLNASSPKMDTVKLILNIKIESPVVSIPRSPGNPELLVGHLGQIFIQNFVSDDDLSRSDKLQV 1419 HSP 3 Score: 172.17 bits (435), Expect = 1.639e-41 Identity = 144/474 (30.38%), Postives = 237/474 (50.00%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILKD-----GDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENT-DHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFP--ANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISF-----QPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
CLLD + +DL +MD+++A R + + DL F +R + L+E+C + L + RNL+ ISH PD I+G LSSV S+ + Y+ IRG+L +NLGE D ++ Y Q DP +HT L+G Y ++ + + +V L L+ +G D N +L R +F +SK +ESFS+ +K+I LV + D R++ A + NVF I+ P K + + Q+ + + S D + F V LN ++ +IFDW+ + + L T E++ H + + KP + S V+ ++ +II E K E+ +NV + F ++E+ S T+A+IL +YK + +R PFS S+ + SC + ++ LSII+PV I I L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 2126 CLLDCIIVDLQDMDIFAAERFSMDFREGFDDFRADLIFPSYFVRQVGGSLLTERCRMKLQLERNLDKEISHAVPDLSIHGNLSSVHCSLDLSDYKLIRGLLENNLGEPVEDFMRPYDLQ----DPKIHTVLSGDVYTSVSFLIDMINVSLELLEE-KGKDGLNN-SLARFDFKKSKFLFESFSNQTKSINLVSHSMMAYDTRFAGQKPLAAHKPNVFNCILQPAKTS----SNPGSLQIELHHRSTKDTSCFTVVLNNLRVFLIFDWLMLVHKF----------LQTPAESKKHVHSSSSEEQKPSNASPVIPKTVKSGVVTKRSSVQIIPE--KQLEVKINVTGTEFVVVEDMSSCDTNAIILKGTTVLTYKPKLADR--PFSGSLFGIEVFSCRVGNELDTALSIIDPVHIQIDLVGN-SAYQNSSGLMDAFNTEDFPPI-LEIQLPALDIRLSYNDVQLFLAIAKSIPKQA 2573 HSP 4 Score: 159.073 bits (401), Expect = 1.582e-37 Identity = 106/306 (34.64%), Postives = 166/306 (54.25%), Query Frame = 2
Query: 4586 NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRF------YDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTK--DGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDE 5479
+ L I + L+DKK FS+ I+R VD DFN L I+++ WV++LDFFG+GS P D + +P + + E NTKL++ + SL+L+N + +N++ L +Y + +G++ L GS+ L DL+ G +Y +R + + L F++FKYGLPD +L RE DMR+ +++ + Y+HTQRF EL F Q+F Q QD L R G+ V AQRS+RI + I AP+LL+P S++S +++ +LGK + N+F F + L D+
Sbjct: 1777 DSLVHINVFLVDKKHPEFSSLYKRINRSVDVDFNCLDILITLQTWVVILDFFGIGSTADNHAVKQQPSDTRQAPKSETNMPLVSEK------EHTNTKLDLKVHSLSLVLKNTRSELAKANVS-------KLTSYLEMIEGDLALQGSIGSLSLSDLTVHGEVYTERF--TTKGEEALIFYIFKYGLPDLALKREYDMRVSLRMA-SVQYVHTQRFQAELVAFIQHFTQLQDVLGRQRAAIQGQTVRNQAQRSSRILLDIEAGAPVLLIPESSWSSKLIVANLGKLRVRNKFLFAGTPETFSLKDK 2066
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Mouse
Match: Vps13d (vacuolar protein sorting 13D [Source:MGI Symbol;Acc:MGI:2448530]) HSP 1 Score: 616.305 bits (1588), Expect = 3.311e-176 Identity = 473/1579 (29.96%), Postives = 750/1579 (47.50%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERNVAFCDCRK---------------------RHNNLFVPFLIVNELGCPIEYRKLSL----------DNPPMYNEIWNA--NKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQ-----NKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS---CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGM----KCYINASCKNAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID-----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG---RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V +C IDDC+D DVPL ++ F+ + + N +G LS DYYN GW P IEPWP ++ W + K ++ L+ +I+S L+ +T + W ++ + + + + FVPF + N GC + + L+ +P + E A + + ++W V T + F+ E AR K+R+ +H H++++++ GW + PV+VDK+G F+R+ D + + + +R+V + +GS + I +RS L + N L P+E + V+ + P +PL + +Q RP +F+ + + W V ++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K +R+G M P W + SL+ +RA V Q NRP L+YNIG++++ GRG++I+T MVI RYLLDN+SS L F Q Q N I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITL+GA YR+ F+D D LP P++++N + + V F Q+ V+E R + P + YA DEPTLPP I L ++G S C ++N + LYY+NF+YIA +F + LV DV+P ++V L +K RSQLW + L HEGS PH+P K RS + +LD+ LA + + + ++L K D RR Q W F EG + G+ K ++ A V G ++ E P+ + F RPGSG + + V+ DGPTR +++ D + +E +++ +L + T +E+ +EG GV LIN +PEEL++A +++ + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC + LLP
Sbjct: 2704 ESVCICFIDDCMDCDVPLAELTFSRLNFLQHIRTNPEGFAHFTLSGDYYNRALSGWEPFIEPWPCSVSWQQQASSRLHPPRLKLEAKAKRRLDINITSVLIDQYISTKESWLADYCKEDKETESAKTEDWMGSSVDPPCFGQTEVKTPKRRQPFVPFALRNHTGCTLWFATLTTTPTRAALSHSGSPGVVPEGNGAFLDDAHNVSEWREV--LTGEEIPFEFE--ARGKLRHRH--THDLRIHQLQVRVNGWEQVSPVSVDKVGTFFRYAAPDKNSSSSTIGSPSSRTNIIHPQVYFSSLPPVRVVFAVTMEGSARKVITVRSALIVKNRLETPMELRLDSPSAPDKPVVLPAIMPGDSFAVPLHLTSWRLQARPKGMG------------VFFCKVPIH-----WTNV------VKTAEVSSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALPTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIACRAEGSLKIFISAPYWLINKTGLPLIFRQDN-AKTDAAGQFEEHELARSLSPLLFCYADKEQPNLCTMRIGRGIHPEGM----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGRYIDTCMVIFAPRYLLDNKSSHKLAFAQREFARGQGTANPNGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCIWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLRGATYRISFSDTDQLPPPFRIDNFSKVPVVFTQHGVAEPRLRTE--VKPMMSLDYAWDEPTLPPFITLTVKGAGSSEINC-NMNDFQNNRQLYYENFIYIAATYTFSGLQEGKGRPVASHKAITCAELVLDVSPKTQRVILKKKEPGKRSQLWRMTGTGMLAHEGSAVPHNPNKPSATRSIEGSAILDIAGLA---AVTDNRYEPLMLRKPDRRRSTTQTWSFHEGKLTCGLHGLVVQAKGGLSGLFDGAEVVLGPDSSMELLGPVPPEQQFANQKMRPGSGMLSIRVIPDGPTRALQITDFCQRKSERSSYEVEELPVTEQELQKLRNPDTHQELEVLVRLEGGVGVSLINKVPEELVFASLTGINIHYTQLAASHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMVTAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENIHEKTAEQGGTPTRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHAATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGLGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGPRTQAQRVRKPRCCTGPQGLLP 4212 HSP 2 Score: 275.789 bits (704), Expect = 5.685e-73 Identity = 289/1188 (24.33%), Postives = 503/1188 (42.34%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEH------TRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRNNL---ESDLELDCLLQLREVAMEHIGRK---SRDLLQNAVNIPAP--------------QQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFT-RDKIFLRGEFSLEGIKLKLLKD-------TEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-------------NPDYK----NDSFRYVVSSREHSKV-----PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEK---GS---SDIRWWVSLKISAPKIILP-NFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESD-----------------LESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVT-----------------------NFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLS--------ESIGDPIIEFTYKIS----QKSGSSDIQR-------------------------------QINLIFSDLDVIANQETLTELLSFMRLLFSND 3088
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+GLIG+VTL+IP+ R W I I +++I GP EKE E KK LL ++E +W+K G+ W S AS+ RI+ N+++++++VH+R+ED T PS+ FA G+ I S +++ E K + K++D++ SIY D C ++ ++ ++ + + E I+ P+ ++KRN + PL H PRI +Q+E + + LSQ QY+ +A E ER +RQ K++P+V+V ++ +EWW FA+ + R + L RAR+ V Y Y + L K L A+ + + E + + L LRE+ + ++ + L + + P Q W WG + SPE T++ E P + +E+ + D +N +T RD +F + L+ + LL E +++ ++ + ES P+ +S + + L LF+RDL NP + + SF +S + S P +F + +E NP+ S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ L++ G RW V L ISAP++I P +F K P V++DLGR+ ++N + +S +A E + D S TP PF +E QA + T +Y+ YS+ ++ +VG NW ++ T ++E FN L L+++ + + P ++ N+ L I I++ K+ K C +T + ++ N S V ++ TR E+ + + F ++ + +ES R ++ L + T + +S+ + L+L D G+ D ++ASHK L D P + P + F ++ Q + S +++ I+L ++LD+I N ET+ EL+ F++ F +
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSVALLKGAVELENLPLKKDALKEL-ELPFEVKAGLIGKVTLQIPFYRPHVDPWVISISGLHVIGGPEKIQDFNDEKEKLLERERKKALLQALEERWKK-----GE------PYWYSVTASVVTRIVENIELKIQDVHLRFEDGVTNPSRPFAFGICIKNVSMQNAANEPVQKL-------MRKKRLDVAEFSIYWDVNCTLLGDLPQAELQEAMARSM-ESRSHHYILEPVCASALLKRNCSKEPLRSRHSPRIECDIQLETIPLKLSQLQYRQIMAFLKELERKERQLKFRKWKPKVAVSKNCREWWYFALNANLYEIREQRKCWTWDFLLHRARDAVFYTDRYFNKL--KGGVLSADDKEEMCRIEEEQSFEELKILRELVHDRFYKQEELAESLREPQFDSPGTSPGDPDGSGGSGMLQYLQSWFPGWGGWYGQQSPE---GKVVEGLTAESHEHWTPEEILGTEEFFDP-------TADASCMNTYTKRDHVFAKLNVQLQRGTVTLLHKEQGTAHANESAFMQLEFSDVKLLAESLPRRNSSLLLVRLGGLFLRDLATEGTMFPLLVFPNPQKEVGRVSQSFGLQTASEDRSDQYSAADPDNPVFEMLYERNPVHS----HFERRLHVSTRPLNIIYNPQAIKKVADFFYKGKVHTSGFGYQSELELRVAEAARRQYDKLKVQTKAEIRQTLDRLLVGDFIEESKRWTVRLDISAPQVIFPDDFTFKN--PVLVVVDLGRMLLTNTQEDSRRKSGDGSASEENQFSDDEYKTPLATPPSTPPPETSSSNGEKTP---PFSGVE--FSEEQLQAHLMS---TQMYERYSLTFMDLQVMVGRVRDNWKRVQDIDVGPTH------VVEKFNVHLQLERRLIYTSDPKYPGAVLSGNLPDLKIHINEDKISALKNCFALLTTPETKPSDTQIREKIFPQEGPRGSLQDSVMNLTQSIVMLEQHTR--------EVLVESQLLLAEFKVNCMQLGVESSGRYISVLKVFGTNAHFVKRPYDAEVSLTVHGLLLVDTMQTYGADFDLLMASHKNLSFDIPTGSLRDSRAQSPVSGPNVAFQTDVTTLNDQSATSVSLEKILTKEQESLIKLEYQFVSSECPSMNLDSTLQVISLQVNNLDIILNPETIVELIGFLQKSFPKE 1128 HSP 3 Score: 171.4 bits (433), Expect = 2.176e-41 Identity = 144/470 (30.64%), Postives = 234/470 (49.79%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILK-----DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGEN-TDHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSS-FPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQ---PVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQ 6841
CLLD + +DL +MD+++A R K GDL F +R L+E C L L V RNL+ ISHT D ++G LSSV S+ + +Y+ IRG+L +NLGE D ++ Y Q DP +HT L+G Y M + + +V L L +G + +L R +F + KL YESFS+ +K+I LV + D RYS P++ NVF I P K++ ++ I Q+ + + S D + F V LN ++ +IFDW+ + +++ T T +R + + +S ++ + PV +H E+ +NV + F ++E+ S T+A+IL +YK R +R PFS S+ + SC + ++ LSI++PV+I ++ L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 2146 CLLDCIVVDLQDMDIFAAERHPREGSKASEHSSGDLIFPSYFVRQTGGSLLTEPCRLKLQVERNLDKEISHTVADISVHGSLSSVHCSLDLCKYKLIRGLLENNLGEPIEDFMRPYDLQ----DPRIHTVLSGEVYTCMCFLIDMVNVSLELKDP-KGKEGTG--SLARFDFKKCKLLYESFSNQTKSINLVSHSMMAFDTRYSGQKPSHGMTNVFNCIFQPSKSSSATQGSI---QIELHFRSTKDSSCFTVVLNNLRVFLIFDWLLLVHDFLHTPSDIKKQNVTPARHRNASSESAVVPKTVKSGVVTKRSSLPVSNERHL-----------EVKVNVTGTEFVVVEDVSCFDTNAIILKGTTVLTYKPRFVDR--PFSGSLFGIEVFSCRLGNEQDTALSIVDPVQIQVELVGN-SSYQNSSGLMDAFNSEDFPPI-LEIQLQALDIRLSYNDVQLFLAIAKSIPEQ 2590 HSP 4 Score: 162.925 bits (411), Expect = 7.672e-39 Identity = 158/542 (29.15%), Postives = 268/542 (49.45%), Query Frame = 2
Query: 3980 KAVYEQVLKSMDNLVYDDE---------------DEIPK------PFIEFEDENMQEDQNPDP--------SENEIPIVLQSN--ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSS---SNIGQENLQEKSVSCPDLLE-QLESSYSNLTSTLIPPPSPFYKYSK-----RKKSLSNSVDIQNCAAFELKLPKNTV--------KSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVK----DLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTK--DGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF 5443
K VYEQVL++MDNLVY ++ +P+ P +E ++ + + E + +Q+N IS+++++L D ++ ++ L Q +EF + + I++A+ + + +L+ K L+ S + S++ Q+ + S SCP + + +L S + P+ F Y + RKK + Q+ + + P +V KS + L I I L+DKK FS+ I+R +D DFN L I+++ WV++LDFFG+GS D +K LL K S + + E +E +N+KL++ + SL+L T + +N++ L A+ + +G++ L GS+ L DL+P G Y +R F S ++ ++ F FKYG PDP L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V AQR +RI + I AP+LL+P S+ S+++++ +LGK + N+F F
Sbjct: 1534 KHVYEQVLQTMDNLVYSEDLNKFAASAPTSPCPNSPLPQLSSCGEPCVEKKENGLFSHSSFSSTSQKSLSVKEAKAFTQIQANFCISELQVQLSGDLTLGAQG-LVSLKFQDFEVEFSKDHPQTLSIQIALRSLLMEDLLEKNPDSKYKSLMVSRGAPKPSSLAQK--EYLSQSCPSVSSVEYPDMPRSLPSHMEEAPNVFQLYQRPISTCRKKP----KEAQDKSYPQTPPPSPSVDEPNMLVGKSKFDDSLVHINIFLVDKKHPEFSSSYGRINRSIDVDFNCLDVLITLQTWVVILDFFGIGST-ADNHAMKVPPEGLLQNVKAESSAS--MESEHQEPVNSKLDLKVHSLSLVLSKATSELAKANVS-------KLMAHMEMIEGDLALQGSIGSLSLSDLTPHGDFYRER-FTTSGEEALI-FQTFKYGQPDPLLQREHDIRVSLQMA-SVQYVHTQRFQAEVVAFIQHFTQLQDILGRQRAAIEGQTVRDQAQRCSRILLDIEAGAPVLLIPESSRSNNLIVANLGKLKVKNKFLF 2055
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Mouse
Match: Vps13d (vacuolar protein sorting 13D [Source:MGI Symbol;Acc:MGI:2448530]) HSP 1 Score: 607.831 bits (1566), Expect = 3.972e-174 Identity = 475/1603 (29.63%), Postives = 751/1603 (46.85%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--------------------------QERNVAFCDCRK-------------------RHNNLFVPFLIVNELGCPIEYRKLSL----------DNPPMYNEIWNA--NKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQ-----NKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS---CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGM----KCYINASCKNAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID-----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG---RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V +C IDDC+D DVPL ++ F+ + + N +G LS DYYN GW P IEPWP ++ W + K ++ L+ +I+S L+ +T + W ++ Q + + R + FVPF + N GC + + L+ +P + E A + + ++W V T + F+ E AR K+R+ +H H++++++ GW + PV+VDK+G F+R+ D + + + +R+V + +GS + I +RS L + N L P+E + V+ + P +PL + +Q RP +F+ + + W V ++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K +R+G M P W + SL+ +RA V Q NRP L+YNIG++++ GRG++I+T MVI RYLLDN+SS L F Q Q N I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITL+GA YR+ F+D D LP P++++N + + V F Q+ V+E R + P + YA DEPTLPP I L ++G S C ++N + LYY+NF+YIA +F + LV DV+P ++V L +K RSQLW + L HEGS PH+P K RS + +LD+ LA + + + ++L K D RR Q W F EG + G+ K ++ A V G ++ E P+ + F RPGSG + + V+ DGPTR +++ D + +E +++ +L + T +E+ +EG GV LIN +PEEL++A +++ + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC + LLP
Sbjct: 1532 ESVCICFIDDCMDCDVPLAELTFSRLNFLQHIRTNPEGFAHFTLSGDYYNRALSGWEPFIEPWPCSVSWQQQASSRLHPPRLKLEAKAKRRLDINITSVLIDQYISTKESWLADYCKEDKETESAKTEDWMGSSVDPPCFGQSLPLVYLRTRSTASLTNLEHQIYAREVKTPKRRQPFVPFALRNHTGCTLWFATLTTTPTRAALSHSGSPGVVPEGNGAFLDDAHNVSEWREV--LTGEEIPFEFE--ARGKLRHRH--THDLRIHQLQVRVNGWEQVSPVSVDKVGTFFRYAAPDKNSSSSTIGSPSSRTNIIHPQVYFSSLPPVRVVFAVTMEGSARKVITVRSALIVKNRLETPMELRLDSPSAPDKPVVLPAIMPGDSFAVPLHLTSWRLQARPKGMG------------VFFCKVPIH-----WTNV------VKTAEVSSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALPTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIACRAEGSLKIFISAPYWLINKTGLPLIFRQD-NAKTDAAGQFEEHELARSLSPLLFCYADKEQPNLCTMRIGRGIHPEGM----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGRYIDTCMVIFAPRYLLDNKSSHKLAFAQREFARGQGTANPNGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCIWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLRGATYRISFSDTDQLPPPFRIDNFSKVPVVFTQHGVAEPRLRTE--VKPMMSLDYAWDEPTLPPFITLTVKGAGSSEINC-NMNDFQNNRQLYYENFIYIAATYTFSGLQEGKGRPVASHKAITCAELVLDVSPKTQRVILKKKEPGKRSQLWRMTGTGMLAHEGSAVPHNPNKPSATRSIEGSAILDIAGLA---AVTDNRYEPLMLRKPDRRRSTTQTWSFHEGKLTCGLHGLVVQAKGGLSGLFDGAEVVLGPDSSMELLGPVPPEQQFANQKMRPGSGMLSIRVIPDGPTRALQITDFCQRKSERSSYEVEELPVTEQELQKLRNPDTHQELEVLVRLEGGVGVSLINKVPEELVFASLTGINIHYTQLAASHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMVTAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENIHEKTAEQGGTPTRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHAATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGLGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGPRTQAQRVRKPRCCTGPQGLLP 3064 HSP 2 Score: 171.785 bits (434), Expect = 1.445e-41 Identity = 144/470 (30.64%), Postives = 234/470 (49.79%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILK-----DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGEN-TDHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSS-FPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQ---PVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQ 6841
CLLD + +DL +MD+++A R K GDL F +R L+E C L L V RNL+ ISHT D ++G LSSV S+ + +Y+ IRG+L +NLGE D ++ Y Q DP +HT L+G Y M + + +V L L +G + +L R +F + KL YESFS+ +K+I LV + D RYS P++ NVF I P K++ ++ I Q+ + + S D + F V LN ++ +IFDW+ + +++ T T +R + + +S ++ + PV +H E+ +NV + F ++E+ S T+A+IL +YK R +R PFS S+ + SC + ++ LSI++PV+I ++ L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 974 CLLDCIVVDLQDMDIFAAERHPREGSKASEHSSGDLIFPSYFVRQTGGSLLTEPCRLKLQVERNLDKEISHTVADISVHGSLSSVHCSLDLCKYKLIRGLLENNLGEPIEDFMRPYDLQ----DPRIHTVLSGEVYTCMCFLIDMVNVSLELKDP-KGKEGTG--SLARFDFKKCKLLYESFSNQTKSINLVSHSMMAFDTRYSGQKPSHGMTNVFNCIFQPSKSSSATQGSI---QIELHFRSTKDSSCFTVVLNNLRVFLIFDWLLLVHDFLHTPSDIKKQNVTPARHRNASSESAVVPKTVKSGVVTKRSSLPVSNERHL-----------EVKVNVTGTEFVVVEDVSCFDTNAIILKGTTVLTYKPRFVDR--PFSGSLFGIEVFSCRLGNEQDTALSIVDPVQIQVELVGN-SSYQNSSGLMDAFNSEDFPPI-LEIQLQALDIRLSYNDVQLFLAIAKSIPEQ 1418 HSP 3 Score: 163.696 bits (413), Expect = 4.516e-39 Identity = 158/542 (29.15%), Postives = 268/542 (49.45%), Query Frame = 2
Query: 3980 KAVYEQVLKSMDNLVYDDE---------------DEIPK------PFIEFEDENMQEDQNPDP--------SENEIPIVLQSN--ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSS---SNIGQENLQEKSVSCPDLLE-QLESSYSNLTSTLIPPPSPFYKYSK-----RKKSLSNSVDIQNCAAFELKLPKNTV--------KSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVK----DLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTK--DGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF 5443
K VYEQVL++MDNLVY ++ +P+ P +E ++ + + E + +Q+N IS+++++L D ++ ++ L Q +EF + + I++A+ + + +L+ K L+ S + S++ Q+ + S SCP + + +L S + P+ F Y + RKK + Q+ + + P +V KS + L I I L+DKK FS+ I+R +D DFN L I+++ WV++LDFFG+GS D +K LL K S + + E +E +N+KL++ + SL+L T + +N++ L A+ + +G++ L GS+ L DL+P G Y +R F S ++ ++ F FKYG PDP L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V AQR +RI + I AP+LL+P S+ S+++++ +LGK + N+F F
Sbjct: 362 KHVYEQVLQTMDNLVYSEDLNKFAASAPTSPCPNSPLPQLSSCGEPCVEKKENGLFSHSSFSSTSQKSLSVKEAKAFTQIQANFCISELQVQLSGDLTLGAQG-LVSLKFQDFEVEFSKDHPQTLSIQIALRSLLMEDLLEKNPDSKYKSLMVSRGAPKPSSLAQK--EYLSQSCPSVSSVEYPDMPRSLPSHMEEAPNVFQLYQRPISTCRKKP----KEAQDKSYPQTPPPSPSVDEPNMLVGKSKFDDSLVHINIFLVDKKHPEFSSSYGRINRSIDVDFNCLDVLITLQTWVVILDFFGIGST-ADNHAMKVPPEGLLQNVKAESSAS--MESEHQEPVNSKLDLKVHSLSLVLSKATSELAKANVS-------KLMAHMEMIEGDLALQGSIGSLSLSDLTPHGDFYRER-FTTSGEEALI-FQTFKYGQPDPLLQREHDIRVSLQMA-SVQYVHTQRFQAEVVAFIQHFTQLQDILGRQRAAIEGQTVRDQAQRCSRILLDIEAGAPVLLIPESSRSNNLIVANLGKLKVKNKFLF 883
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Mouse
Match: Vps13d (vacuolar protein sorting 13D [Source:MGI Symbol;Acc:MGI:2448530]) HSP 1 Score: 607.831 bits (1566), Expect = 1.155e-173 Identity = 475/1604 (29.61%), Postives = 751/1604 (46.82%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--------------------------QERNVAFCDCRK--------------------RHNNLFVPFLIVNELGCPIEYRKLSL----------DNPPMYNEIWNA--NKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQ-----NKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS---CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGM----KCYINASCKNAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID-----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG---RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V +C IDDC+D DVPL ++ F+ + + N +G LS DYYN GW P IEPWP ++ W + K ++ L+ +I+S L+ +T + W ++ Q + + R + FVPF + N GC + + L+ +P + E A + + ++W V T + F+ E AR K+R+ +H H++++++ GW + PV+VDK+G F+R+ D + + + +R+V + +GS + I +RS L + N L P+E + V+ + P +PL + +Q RP +F+ + + W V ++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K +R+G M P W + SL+ +RA V Q NRP L+YNIG++++ GRG++I+T MVI RYLLDN+SS L F Q Q N I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITL+GA YR+ F+D D LP P++++N + + V F Q+ V+E R + P + YA DEPTLPP I L ++G S C ++N + LYY+NF+YIA +F + LV DV+P ++V L +K RSQLW + L HEGS PH+P K RS + +LD+ LA + + + ++L K D RR Q W F EG + G+ K ++ A V G ++ E P+ + F RPGSG + + V+ DGPTR +++ D + +E +++ +L + T +E+ +EG GV LIN +PEEL++A +++ + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC + LLP
Sbjct: 2710 ESVCICFIDDCMDCDVPLAELTFSRLNFLQHIRTNPEGFAHFTLSGDYYNRALSGWEPFIEPWPCSVSWQQQASSRLHPPRLKLEAKAKRRLDINITSVLIDQYISTKESWLADYCKEDKETESAKTEDWMGSSVDPPCFGQSLPLVYLRTRSTASLTNLEHQIYARAEVKTPKRRQPFVPFALRNHTGCTLWFATLTTTPTRAALSHSGSPGVVPEGNGAFLDDAHNVSEWREV--LTGEEIPFEFE--ARGKLRHRH--THDLRIHQLQVRVNGWEQVSPVSVDKVGTFFRYAAPDKNSSSSTIGSPSSRTNIIHPQVYFSSLPPVRVVFAVTMEGSARKVITVRSALIVKNRLETPMELRLDSPSAPDKPVVLPAIMPGDSFAVPLHLTSWRLQARPKGMG------------VFFCKVPIH-----WTNV------VKTAEVSSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALPTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIACRAEGSLKIFISAPYWLINKTGLPLIFRQDN-AKTDAAGQFEEHELARSLSPLLFCYADKEQPNLCTMRIGRGIHPEGM----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGRYIDTCMVIFAPRYLLDNKSSHKLAFAQREFARGQGTANPNGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCIWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLRGATYRISFSDTDQLPPPFRIDNFSKVPVVFTQHGVAEPRLRTE--VKPMMSLDYAWDEPTLPPFITLTVKGAGSSEINC-NMNDFQNNRQLYYENFIYIAATYTFSGLQEGKGRPVASHKAITCAELVLDVSPKTQRVILKKKEPGKRSQLWRMTGTGMLAHEGSAVPHNPNKPSATRSIEGSAILDIAGLA---AVTDNRYEPLMLRKPDRRRSTTQTWSFHEGKLTCGLHGLVVQAKGGLSGLFDGAEVVLGPDSSMELLGPVPPEQQFANQKMRPGSGMLSIRVIPDGPTRALQITDFCQRKSERSSYEVEELPVTEQELQKLRNPDTHQELEVLVRLEGGVGVSLINKVPEELVFASLTGINIHYTQLAASHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMVTAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENIHEKTAEQGGTPTRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHAATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGLGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGPRTQAQRVRKPRCCTGPQGLLP 4243 HSP 2 Score: 281.567 bits (719), Expect = 9.288e-75 Identity = 290/1188 (24.41%), Postives = 504/1188 (42.42%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEH------TRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRNNL---ESDLELDCLLQLREVAMEHIGRK---SRDLLQNAVNIPAP--------------QQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFT-RDKIFLRGEFSLEGIKLKLLKD-------TEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-------------NPDYK----NDSFRYVVSSREHSKV-----PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEK---GS---SDIRWWVSLKISAPKIILP-NFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESD-----------------LESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVT-----------------------NFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLS--------ESIGDPIIEFTYKIS----QKSGSSDIQR-------------------------------QINLIFSDLDVIANQETLTELLSFMRLLFSND 3088
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+GLIG+VTL+IP+ R W I I +++I GP EKE E KK LL ++E +W+ + RQQ W S AS+ RI+ N+++++++VH+R+ED T PS+ FA G+ I S +++ E K + K++D++ SIY D C ++ ++ ++ + + E I+ P+ ++KRN + PL H PRI +Q+E + + LSQ QY+ +A E ER +RQ K++P+V+V ++ +EWW FA+ + R + L RAR+ V Y Y + L K L A+ + + E + + L LRE+ + ++ + L + + P Q W WG + SPE T++ E P + +E+ + D +N +T RD +F + L+ + LL E +++ ++ + ES P+ +S + + L LF+RDL NP + + SF +S + S P +F + +E NP+ S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ L++ G RW V L ISAP++I P +F K P V++DLGR+ ++N + +S +A E + D S TP PF +E QA + T +Y+ YS+ ++ +VG NW ++ T ++E FN L L+++ + + P ++ N+ L I I++ K+ K C +T + ++ N S V ++ TR E+ + + F ++ + +ES R ++ L + T + +S+ + L+L D G+ D ++ASHK L D P + P + F ++ Q + S +++ I+L ++LD+I N ET+ EL+ F++ F +
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSVALLKGAVELENLPLKKDALKEL-ELPFEVKAGLIGKVTLQIPFYRPHVDPWVISISGLHVIGGPEKIQDFNDEKEKLLERERKKALLQALEERWKSERRQQKGE-----PYWYSVTASVVTRIVENIELKIQDVHLRFEDGVTNPSRPFAFGICIKNVSMQNAANEPVQKL-------MRKKRLDVAEFSIYWDVNCTLLGDLPQAELQEAMARSM-ESRSHHYILEPVCASALLKRNCSKEPLRSRHSPRIECDIQLETIPLKLSQLQYRQIMAFLKELERKERQLKFRKWKPKVAVSKNCREWWYFALNANLYEIREQRKCWTWDFLLHRARDAVFYTDRYFNKL--KGGVLSADDKEEMCRIEEEQSFEELKILRELVHDRFYKQEELAESLREPQFDSPGTSPGDPDGSGGSGMLQYLQSWFPGWGGWYGQQSPE---GKVVEGLTAESHEHWTPEEILGTEEFFDPT-------ADASCMNTYTKRDHVFAKLNVQLQRGTVTLLHKEQGTAHANESAFMQLEFSDVKLLAESLPRRNSSLLLVRLGGLFLRDLATEGTMFPLLVFPNPQKEVGRVSQSFGLQTASEDRSDQYSAADPDNPVFEMLYERNPVHS----HFERRLHVSTRPLNIIYNPQAIKKVADFFYKGKVHTSGFGYQSELELRVAEAARRQYDKLKVQTKAEIRQTLDRLLVGDFIEESKRWTVRLDISAPQVIFPDDFTFKN--PVLVVVDLGRMLLTNTQEDSRRKSGDGSASEENQFSDDEYKTPLATPPSTPPPETSSSNGEKTP---PFSGVE--FSEEQLQAHLMS---TQMYERYSLTFMDLQVMVGRVRDNWKRVQDIDVGPTH------VVEKFNVHLQLERRLIYTSDPKYPGAVLSGNLPDLKIHINEDKISALKNCFALLTTPETKPSDTQIREKIFPQEGPRGSLQDSVMNLTQSIVMLEQHTR--------EVLVESQLLLAEFKVNCMQLGVESSGRYISVLKVFGTNAHFVKRPYDAEVSLTVHGLLLVDTMQTYGADFDLLMASHKNLSFDIPTGSLRDSRAQSPVSGPNVAFQTDVTTLNDQSATSVSLEKILTKEQESLIKLEYQFVSSECPSMNLDSTLQVISLQVNNLDIILNPETIVELIGFLQKSFPKE 1134 HSP 3 Score: 171.014 bits (432), Expect = 2.534e-41 Identity = 144/470 (30.64%), Postives = 234/470 (49.79%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILK-----DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGEN-TDHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSS-FPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQ---PVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQ 6841
CLLD + +DL +MD+++A R K GDL F +R L+E C L L V RNL+ ISHT D ++G LSSV S+ + +Y+ IRG+L +NLGE D ++ Y Q DP +HT L+G Y M + + +V L L +G + +L R +F + KL YESFS+ +K+I LV + D RYS P++ NVF I P K++ ++ I Q+ + + S D + F V LN ++ +IFDW+ + +++ T T +R + + +S ++ + PV +H E+ +NV + F ++E+ S T+A+IL +YK R +R PFS S+ + SC + ++ LSI++PV+I ++ L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 2152 CLLDCIVVDLQDMDIFAAERHPREGSKASEHSSGDLIFPSYFVRQTGGSLLTEPCRLKLQVERNLDKEISHTVADISVHGSLSSVHCSLDLCKYKLIRGLLENNLGEPIEDFMRPYDLQ----DPRIHTVLSGEVYTCMCFLIDMVNVSLELKDP-KGKEGTG--SLARFDFKKCKLLYESFSNQTKSINLVSHSMMAFDTRYSGQKPSHGMTNVFNCIFQPSKSSSATQGSI---QIELHFRSTKDSSCFTVVLNNLRVFLIFDWLLLVHDFLHTPSDIKKQNVTPARHRNASSESAVVPKTVKSGVVTKRSSLPVSNERHL-----------EVKVNVTGTEFVVVEDVSCFDTNAIILKGTTVLTYKPRFVDR--PFSGSLFGIEVFSCRLGNEQDTALSIVDPVQIQVELVGN-SSYQNSSGLMDAFNSEDFPPI-LEIQLQALDIRLSYNDVQLFLAIAKSIPEQ 2596 HSP 4 Score: 162.925 bits (411), Expect = 8.567e-39 Identity = 158/542 (29.15%), Postives = 268/542 (49.45%), Query Frame = 2
Query: 3980 KAVYEQVLKSMDNLVYDDE---------------DEIPK------PFIEFEDENMQEDQNPDP--------SENEIPIVLQSN--ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSS---SNIGQENLQEKSVSCPDLLE-QLESSYSNLTSTLIPPPSPFYKYSK-----RKKSLSNSVDIQNCAAFELKLPKNTV--------KSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVK----DLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTK--DGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF 5443
K VYEQVL++MDNLVY ++ +P+ P +E ++ + + E + +Q+N IS+++++L D ++ ++ L Q +EF + + I++A+ + + +L+ K L+ S + S++ Q+ + S SCP + + +L S + P+ F Y + RKK + Q+ + + P +V KS + L I I L+DKK FS+ I+R +D DFN L I+++ WV++LDFFG+GS D +K LL K S + + E +E +N+KL++ + SL+L T + +N++ L A+ + +G++ L GS+ L DL+P G Y +R F S ++ ++ F FKYG PDP L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V AQR +RI + I AP+LL+P S+ S+++++ +LGK + N+F F
Sbjct: 1540 KHVYEQVLQTMDNLVYSEDLNKFAASAPTSPCPNSPLPQLSSCGEPCVEKKENGLFSHSSFSSTSQKSLSVKEAKAFTQIQANFCISELQVQLSGDLTLGAQG-LVSLKFQDFEVEFSKDHPQTLSIQIALRSLLMEDLLEKNPDSKYKSLMVSRGAPKPSSLAQK--EYLSQSCPSVSSVEYPDMPRSLPSHMEEAPNVFQLYQRPISTCRKKP----KEAQDKSYPQTPPPSPSVDEPNMLVGKSKFDDSLVHINIFLVDKKHPEFSSSYGRINRSIDVDFNCLDVLITLQTWVVILDFFGIGST-ADNHAMKVPPEGLLQNVKAESSAS--MESEHQEPVNSKLDLKVHSLSLVLSKATSELAKANVS-------KLMAHMEMIEGDLALQGSIGSLSLSDLTPHGDFYRER-FTTSGEEALI-FQTFKYGQPDPLLQREHDIRVSLQMA-SVQYVHTQRFQAEVVAFIQHFTQLQDILGRQRAAIEGQTVRDQAQRCSRILLDIEAGAPVLLIPESSRSNNLIVANLGKLKVKNKFLF 2061
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Mouse
Match: Vps13d (vacuolar protein sorting 13D [Source:MGI Symbol;Acc:MGI:2448530]) HSP 1 Score: 464.151 bits (1193), Expect = 6.518e-138 Identity = 343/1079 (31.79%), Postives = 539/1079 (49.95%), Query Frame = 2
Query: 8075 IETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQ-----NKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS---CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGM----KCYINASCKNAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID-----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG---RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYG 11095
++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K +R+G M P W + SL+ +RA V Q NRP L+YNIG++++ GRG++I+T MVI RYLLDN+SS L F Q Q N I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITL+GA YR+ F+D D LP P++++N + + V F Q+ V+E R + P + YA DEPTLPP I L ++G S C ++N + LYY+NF+YIA +F + LV DV+P ++V L +K RSQLW + L HEGS PH+P K RS + +LD+ LA + + + ++L K D RR Q W F EG + G+ K ++ A V G ++ E P+ + F RPGSG + + V+ DGPTR +++ D + +E +++ +L + T +E+ +EG GV LIN +PEEL++A +++ + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + + AG LA+G
Sbjct: 6 VKTAEVSSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALPTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIACRAEGSLKIFISAPYWLINKTGLPLIFRQD-NAKTDAAGQFEEHELARSLSPLLFCYADKEQPNLCTMRIGRGIHPEGM----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGRYIDTCMVIFAPRYLLDNKSSHKLAFAQREFARGQGTANPNGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCIWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLRGATYRISFSDTDQLPPPFRIDNFSKVPVVFTQHGVAEPRLRTE--VKPMMSLDYAWDEPTLPPFITLTVKGAGSSEINC-NMNDFQNNRQLYYENFIYIAATYTFSGLQEGKGRPVASHKAITCAELVLDVSPKTQRVILKKKEPGKRSQLWRMTGTGMLAHEGSAVPHNPNKPSATRSIEGSAILDIAGLA---AVTDNRYEPLMLRKPDRRRSTTQTWSFHEGKLTCGLHGLVVQAKGGLSGLFDGAEVVLGPDSSMELLGPVPPEQQFANQKMRPGSGMLSIRVIPDGPTRALQITDFCQRKSERSSYEVEELPVTEQELQKLRNPDTHQELEVLVRLEGGVGVSLINKVPEELVFASLTGINIHYTQLAASHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMVTAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENIHEKTAEQGGTPTRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHAATSGEH---LVAGIHGLAHG 1043
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Mouse
Match: Vps13a (vacuolar protein sorting 13A [Source:MGI Symbol;Acc:MGI:2444304]) HSP 1 Score: 135.961 bits (341), Expect = 8.768e-31 Identity = 153/703 (21.76%), Postives = 316/703 (44.95%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQ-GDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSV-ENIKSVLQEKLNE--MPKS-DCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITE-------HTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFR-NNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLW------GRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIP-ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAK-----ARTSESIKKILEKGSS-DIRWWVSLKISAPKIILPNF 2050
++ ++ +L+R +G YV N + G++KG + L+NL +K L +L + P VK G IG + L+IP+ +Q ++E+I+++ P+S + + + + M K E+K +K +Q+ D +K + ++ L +I++NLQ+++ ++H+RYED+ T + + G+ + S +++D W TE ++ K + L + Y + ++ E++K++ +NE +P+ D + PIS ++ N P+I+L + + + I ++ QY + L + + + + + K++P V + KEWW +AI + ++ +R + +Y + Y+ L +K + E LE L++ + R+ A + + + V P +GW+G+LW + ++ P IL++ E S + E + SE ++ + + FEA+ FF L+ + + L ++ + P +L V + L RP + + S + L D+K +++SS + + + + FE NPL T + + I +EPL I++ + +I E+F R +++ A + K A+T+ + ++E D+R + + A +I+P +
Sbjct: 1 MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHEL-DVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETK----QQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCLHDNTE-KLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKALKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSDFDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPE-DNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAI-----GYSETAVDPTLPK-----TFEALKFFVH----------LKSMSIVLRENHQKPELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPDDFKKP---HLLSSLDDTS-----LLQITFEINPLNET----VAQRCTIEAEPLEIIYDARTVNSIVEFF-RPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLR----INVKASYVIVPQY 659
BLAST of Vacuolar protein sorting 13 homolog D vs. UniProt/SwissProt
Match: sp|Q9VU08|VP13D_DROME (Vacuolar protein sorting-associated protein 13D OS=Drosophila melanogaster OX=7227 GN=Vps13D PE=1 SV=3) HSP 1 Score: 805.053 bits (2078), Expect = 0.000e+0 Identity = 1006/4094 (24.57%), Postives = 1778/4094 (43.43%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS--NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEG-KIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLD-----TKCKIMSEVSV-ENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNI--NPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEA---EFRNNLESDLELDCLLQLREVAMEHIGRKS------RDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIPI-------LEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSRE-HSKVPGGEIFALAFETNPLPSTCPPNIKRI---LAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIR----WWVSLKISAPKII-LPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTN------------IYDEYSIKLENVMALVGNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLE----LTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQ------------PLSES----------------------IGDPIIEFT------------YKISQKSGSSDIQ-RQINLIFSDLDVIANQETLTELLSFMR--LLFSNDSLQIF-DQKPETPN----------------------------------TTEFKEFDVSIACKAVSILLIKISSNLASENRFAVSVGRISLSQMN--LLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSR-LKHNIAAVARTAILSSEESTRIGFLNSVKLHLRMLSPILALPRHK-------------------TSNQIILVNLGDIEITSDTQSLQVAFSDAIVISKE------LPIENGGYDIGIC-TDNNAKFPLVNKIDLMLK----FSKLHDPPKIL----IECEXXXXXXXXXXKAVYEQVLKSM------DNLVYDDEDEIPKPFIEFEDENMQEDQNPDPSENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEKSVSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNSVDIQNCAAFELKLPKNTVKSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIV-YIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF--ENNDRSRC--------LLDEMKIDLHEMDVYSAIRITDSILKDGD---LKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHL-KSYKFQAFQTDPILHT-------DLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEID---NCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFT-APYGIVSLATDDENRFDQHVAFYIQSKPQ-SRSISFQPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWS---SLNFD--------NVNTSKPSQPNLTALQKLSKFVDRISIH---HLDDYEFVSVCLIDDCIDADVPLFDIKFN----NVTVDIELGNSK---------GLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFW----LTTKK---FSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDEN-----KWISV-PDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSL--NIPLEAIXXXXXXXXXXXV-VLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNK--VYLICTIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLI----RLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNC---NEDQNKP--IMALPASF-LTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQ---LRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLP--PKSCIPYALDEPTLPPIINLCIEGGTQSCYDIN-KVGPGQNLYYDNFLYIAIRNSFE-----SDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKN-----------AAVFGGFETFKEFYAPL-----VYKNWFRPGSGEVLVSVVTDGPTRVMRLID---KEDESILSQSQLDFNA--------DSSTFS-------NIEMHFSVEGFGVFLINHIP-EELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSK---EAELLEEDFSSLQHQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKK-LFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFE-NARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSS-WKLLPYQTSYSN 11365
M+ LIT++L+ +GKY+EN ++ + L G++ LEN+P++ L F P+ V +G I ++ L+IP +FR+ W I IE ++ I P + NW +EKE + K +LD+ E WR + +Q ++ WL Y ++ I+ N+++++ +VH R+ED TG+ I + + +S D +W + + ++ K V+L +S+Y D KC+ S + E + S E+ + I+ PI KR+ + +PR+S L + +V I +S+ Q + E + + + RP +V +P WW +A H K + ++Y+ Y+ +LN + NL A EF+ +ESD ++ L +R + E + K + +N + P GW+ SP + Q + S + LE DI L E + + D +F G FS++ +K ++ TE + +E+ N + PQ +S+ + I L+ +++ D + Y++ + ++ P + A + + L N + L I S+ L +++ I + ++ + S + + RN A+ ++ +K E S + W ++I AP+II L N+ K VLLD G+L++ + R I V SD + E ++TPCS+P S ++ S + + +T+ N IYD+Y I N+ LV + E K +S L++ FN L L+++ + + P+ ++ +LI +++ + + K +++ N YR ++ R+ NL + + V I FV+ +I+++S R +AEL + + L++ T IS+ + L+L DA + G + ++ASH+ + D P S +GD EF KI + S+ +Q ++ F+ LD+IANQ+T+ E+L+F + LL N IF + PE+P T + +F+V V++ KI+++ S+ S+G I + + H ++ +R+ + ++L L I + + ETK S+ D I+ + F + Y H P FL V CI+ YF R L+ ++ A ++E F+ V+ + + P+ + TS +++ NLG I ++ + F+++ I + L I+ G Y + N P+++ + L+ +S +D K L I+ +L +S ++ YE +L+S+ N V ++ DE+ ++ +++ N D +E+ I +Q ++ ++ L + + ++ L + ++ + + + KD+E+ + + L + L S + ++ S ++N + S SCPDL +S N +S++ PS FY + + K F + E K + +I K G S + + + FN L I +E W + DFFGL S + +V++ P + + I+++ +KL + ++ F+ L ++ N++ + Y+K + G L + DL+ G+IY Q+ + + L F ++K L D L D +I++ V YIHT+RF EL+ F ++ LQ Q + + + + R +++++ I +P+++LP S + ++I LG+FSL N F F +NN S+ +LD M+IDL ++++S R + D + + + +N + +E C L+L + RNL+ + PD + G S + ++++QY+ IR L +N+GE TD + +Y + + L T +++ I + I + L DV L L L + A L ++F +S L + FSD S+ I+L+ I + D R S +N+ NVF I+ P K +R E+ + +C+ + +S + + LN M+ + ++ K+++ +P +V+ A + I KPQ SIS TE +N+ +S E S+L ++A+IL YK N P S ++ + SC + ++ + LSII+P +II+ C+ ++L K IRLS+ D++L ++ R QT P N+ + + + ++ F+ +N + + + Q+ ++ + D +SV +IDDC+DADVPL ++ + N T + N K G + +SV+YYN GW PV E W + W + L KK ++S+ L +++S +++ K W+ +F + R + F+PF + N G P+ ++ P+Y + + + + W SV P+ T FD + + K+R+ + SH H+I ++I GW+ P++VDK+G+F+R + D+ T RIV +I GS Q I ++S L + N L N+ L+ S SS+ V+KPN + +PL + + + H+ S + D F + E ++ C + Q + A + NK +Y I R + + +PGH IT+ P + N L CD+ F + + S + ++ Q S+ LD+F L I +SH +I +L D Q R L + V + S P + + IS+ WI+N+T LPL+Y Q G + T AAG E+E A P F+FS++ S +RLG +NM W + S K LRA + + Y IG+ +R GRG + T V L+ R+ L NRS + L F+Q C N D+ P I++ P FHWP D +Q++ V+ + SK G I S ++ +R N + LR+ I + A + ++FTDA LP P +++N + + + N S+ + + P P+S + Y +D+P I+ + GG + IN K + L Y NF+YIA + +FE + LV V KKV + +K+S RSQLW +SN +L HEGS P Q D +A L+LD++ AP P + +++ + +R Q W F G ++ C+ N ++ AV G E + + RPGSG++ +S DGP + + D K++ L+ L +A D S N+E+ V+G G+ +I P EE+++ ++++ + + +++L + I IQ+DNQ +DA ++ + S +QNA LK + + +F ++ + + P LEEK++LK FL K + ++ F + + + ++ Y NL + + LS T+ LP +L + KK L +L+ F +A I +D Y FET ++ + + HY NQ++ A I GSVDFLGNP+GF +D++ G+ L + V ++VK+ HGI +STAK+ + S + ++ +D E RQR+ E + SG + A AG K L +G+ GG+ S+ G +G G G KG+VG VTKP+ G+LDLA+ +A++ RE +R S P R+ PRC++ + LLP YSN
Sbjct: 1 MLRDLITWVLNTYLGKYLENLNSAQLSVALLSGEVELENIPIRKDALRS-FNLPVEVTAGSIRKIKLQIPVRQFRTSPWCISIEGLFCIICPKNLDNWD-YEKEKLQDLEYKLAVLDTAEAGWRSEKGKQMESYYFSSYNNWLKYGTNMATNIIDNIELKISDVHFRFEDIVDTGKSKICTGIKIGSLTAQSCDCDWTNGSYKMNNNEMNYKLVELKELSVYWDLLHEDIKCQSYSNQEILEKMHSTC-----ELRSHNFIIKPICATARWKRDKCQQVIRTKDKPRVSCELLVPEVVIDISKVQRLQMLDKLSEIRQVKEVRQYRLKRPTCTVESNPIAWWKYATICHGFDFKKNEEKWLMLKENLRYMLLYKSIILNPNENLSAADKEFKAYIESDRKISDLTIMRRICFEKVFTKGFAFESQNEQGKNMLFHWFPNWMGWYA---------NSPSTPNNEQ-----------------DESLKHLEDDILVAL-ENSLQNSSDLKSDAVF--GHFSIKLLKGLVILQTEDKLNDGRNKSMEMQFNNFSAYLQLSPQLTSYTVGISLQEVYLIDKTS--SDTMHNYLIKPQTGNTATPNQLVKNAALQEDILFQLQYENCNHLRFQLNIKSKGLDLIYNEDAIQWLLDFLADSN-----SFKYSPRNRVAKKTDFMKNWNEMFSGNEVNRKIWMFEIEIFAPRIIFLENY--KVSNSLMVLLDFGKLEMRKME---VKRVIPVIESAVTENTSD-DDETYLTPCSTPPASEKSGSESPTLLENPKTESFLNKNVQLEYVLHNKIYDKYLINFTNLQVLVCKYEERWQACLKTSSN---FHLIDKFNINLTLEQRNIFTVDPEYPSFMLFGTCPTILIHGNEELINNCCNIMKPIIKASKEMENIYRGGNT----IYASERIKNLAE-----DDRSRVVIEFVMDQLVIEMQSTERSIAELQIIGARAGLTKEPHETNISMSVHGLLLVDAIQSFGPDFELLVASHRHVGMDSLSGSLKHSAIVSPTSPGSPNFFDRATSPHMITKAVQNIKMGDRSTEFCDDEDSTALISVDIKIVPPNESNSMQLHTTSITFNSLDIIANQDTIIEILNFAKRTLLAQN----IFPSESPESPKEATEAPVDVVDQVDHKSHNEIFFDFFRLNILLLYTIKRDKFNVGRKVGTVTLTEAKINASFQSDLSIIGSLGGIQVIDITSEAFCHPRILSVGRDQILRASDTNKQTVLSQLSNEIYS-NNYNEETK---SDESDAISFQSHWSDKTTCTF-QMRMASASYTHCPRFLRDVNACIT----YFKRSLREFATSIGNKATDMAKE-----FVQQVRA-VEQIGPVYPQRNRQDNWLDIIISSPIIILPISNTSTNVLIANLGKISCSNAVKCDSDEFNESYTIEIKNTYVYSLNIDEGEYSFNVHPAKNKDAIPILHDTAITLQLYAGYSDDNDEDKQLNRFSIKGSMVEALKVSLNRKQYELLLESIRYATNFSNEVLNESDELD------QNGDIEPTANID-AESIISTTIQFSVPVFQINLQ----NEYHNDLINLTFKNFNVKHI-SKGFDKDVEVVLKSV-LMEDLKSDLTSPFRNMVTSVDLEQKIKKN-EMTSSSCPDLPSYC-NSLKNRSSSV---PSCFYNHMQVK-------------VFGGDQKYTSSNKNELGKKKESQTLVIYKSHTGRSAQNGKLEQTSSIQFNCLNLAICVERWYTIFDFFGLVS----------VDNVNEKYPEEMKLVEKHIDKVC-SKLKVSIRSFNFTLIRNESLLSRVNVSNAVFMILQ-DPYSK----IVEGCLGSVSVNDLTKYGNIYKQKFLTSGTE--ALNF-VYKKKLVDLEALNTLDTDSTLRINMSAVHYIHTKRFSTELHVFVKDLLQLQTPV----IRKLKKHGSEQNMRPSKMKLVIQADSPVIVLPSSYNRNEVIIAYLGQFSLKNSFHFASDNNIISKMSATPSKDEILDVMRIDLVNINLFSGERSSMKAKADQEKDRIIIADMNFLRLGQPFFNESCFLHLQLERNLSADAHRVCPDISVQGTFSKLSGIINIQQYKLIRSFLNNNIGEQTDDIYMNYHNNSCTSIERLSTINLMPKNEVSKIVSILISIRILLEDVSLLLA--LNTSQSAAIEPLACIHFLKSTLEIDLFSDGSQDIDLISSNILIVDERNESDKSNE--NVFKNILEPSKKEVRIENSVQVEVHCRKKATFSK------YTIMLNNMRVFALLSFLDQLKSYLQEDSPAPVVNNAANQ-----------IAQKPQIDTSIS--------------------TEYVVNITDSEIIFAEECSRLDSNAIILKSTTVICYKPNSNIV-PLSLDINHLEIFSCTLDAEEESALSIIDPFTLIIELRSN-CL--------NILIQKH------------LNIRLSYVDVKLFSRMARLLPTQTSRPKNVISKADSDLEKAAPLVAMGFEISDCLYAMQINNWRINDAAIWLSQQKQNTYRNPALEMKTAVVDASLISVFIIDDCMDADVPLLEVSLSKFLLNFTFQTQDPNPKETNIRHYSLGNIDTEVSVNYYNRRLSGWEPVAETWESNLNWKYTKGHLDNKKRFEIGISSKQMLKLNVTSTFIELFHMVLKNWTNDFNDNGAK----NFRQRSPFIPFALQNLSGTPLLFK-------PIYAPLGDLTRSDLQQVELIKNWYSVQPNETKT---FDF--SQKSKLRH--VHSHQLNLHQIFVQIHGWTLIGPISVDKVGMFFRTTKLDSQ-----FLTKSRIVFDISLIGSAQKLIKVKSSLGVINKLDRNVFLKMTLKGTHSDGLSSISVIKPNDELSVPLKFIDASLYV-----AHNTSESDAYEDTGFSN----EEILWKACGKDDTRQLL--------------AGYDTNKSILYTFVNISREIYHCKEQNLPGHKITLLPPLKINNMLCCDLMFKIHEHATGR----------INSSESVNIYNVNICQPLNLSITLDNFQLSGQLKI----PVSHRGVIEPKLKLIDIQKRELHVRVSIQSVPGKGMELYISAPVWIINKTGLPLIYKQEGTSHT-AAGQFEEHETARQVAPLMFSFSDQEGSPALVLRLGKAYGSNNM------WCKSFSTHK-DLADRDLRAENTKGS-----YAIGISVRRGRGLYACTTFVTLSPRFHLHNRSGYKLEFMQL-CDIVNYDRPDPRKIISAPVDCNFAFHWPNWDQEQIICVRIPEIECCCWSK---GIPIKDVQSLYINVR----NEWGEMFFLRLEIISKDATFILLFTDARTLPPPIRIDNCSEVVI----NFSQLRSKPVWITPVRPQSSLSYVMDDPLGQQILLMEAPGGNMIEFPINNKNNIKKTLTYTNFIYIAFQGTFERSNEEENTHRQLVLGV--RGKKVVIVEKNSGDRSQLWLMNSNGQLEHEGSTPP---IQTNDANAV-RLVLDLE-KAPNP----MEFTNLVVRTPNKQRVTTQTWRFENGRLM-------CHANMCVQSRFGESGLKPNYEAVVGRTENRASSSKQIPIGQHIVAQKLRPGSGQLELSTKMDGPISTIEICDIKIKQNSVFLTPDLLWMHASLNNRQITDKGKVSFVHEYLINVEL---VKGIGISIIARKPCEEIMFISLDHINCDIVQSALENSLDLNIAYIQIDNQLLDAVSPIALHTQT-SNDLEETQNAVVLKLKMLPSPNKNAIIFKYLTLDLKPSTASLEEKLILKVAS-FLGYGKINRQNLSVQYQFENTDDKPFLQDMKRYYFENLSIGATQVRLSAFTSSKLPVELHETKKALGLTLIKFEDALIELDRYSDKLHFETMDVYRKELKKHYINQVKWHAAAILGSVDFLGNPLGFANDLSEGVSGLIFEGSVKSLVKNVTHGISNSTAKLTETLSDSLGKVVLDDHDNETRQRILELQSNTSGGHLA---AGLKGLGFGLLGGVTSIVRHTYDGATSDGVPGFLSGLGKGLVGTVTKPIIGVLDLASETASAVRETSRDSHRNAPERKRLPRCVTGAPGGLLPL---YSN 3783
BLAST of Vacuolar protein sorting 13 homolog D vs. UniProt/SwissProt
Match: sp|Q5THJ4|VP13D_HUMAN (Vacuolar protein sorting-associated protein 13D OS=Homo sapiens OX=9606 GN=VPS13D PE=1 SV=2) HSP 1 Score: 611.298 bits (1575), Expect = 6.475e-174 Identity = 477/1606 (29.70%), Postives = 749/1606 (46.64%), Query Frame = 2
Query: 7013 EFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNS-KGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--------------------------QERNVAFCDCRK--------------------RHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCG--------------DENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY------------------IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLIC-TIERLNFP-LAPKMV-----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS---CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGM----KCYINASCKNAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLID----------KEDESILSQSQLDFNADSSTFSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENK--SESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGR---KIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
E V +C IDDC+D DVPL ++ F+ + + S +G LS DYYN GW P IEPWP ++ W K ++ L+ +I+S L+ +T + W ++ Q + + R + FVPF + N GC + + L+ P + ++ G + ++W V T + F+ E AR K+R+ +H H++++++ GW + PV+VDK+G F+R+ D + + + +R+V + +GS + I +RS L + N L P+E + V+ + P +PL + +Q RP + F +PI W V ++T S + + EK++ + C I++ N+P P + PGH I + + N LPC++ F +K MP ++P + + T Q+ V L++FP C LLI + ++ V +RLYD R L L +++ R G + + IS+ YW++N+T LPL++ Q +T AAG E+E A S P F +++K +R+G M P W + SL+ +RA V Q NRP L+YNIG++++ GRG++I+T MVI RYLLDN+SS L F Q Q I LP S + FHWPR+D DQLL V+ +D P SGGF+++ NSFH+ +R LRV ITL+GA YR+ F+D D LP P++++N + + V F Q+ V+E R + P + + YA DEPTLPP I L ++G S C ++N + LYY+NF+YIA +F + LV DV+P ++V L +K RSQLW + L HEGS PH+P K RS + +LD+ LA + + + ++L K D RR Q W F EG + G+ K ++ A V G +T E P+ + F RPGSG + + V+ DGPTR +++ D + DE +++ +L + T +E+ +EG G+ LIN +PEEL++A ++V + + + L + I +QVDNQ + ++Y EN+ P+ NA + L +++ + + + +EEK+LLK + F E+E+ E + H+ ++G + Y NLK+ I LSV T+ LP DLK +K FP L+ F +A I +D + R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + +A SG + + AG LA+GI GG+ SV T +G K EG SG G KG+VG VTKPV G LD A+ +A + R+ A S Q R +PRC + LLP
Sbjct: 2709 ESVCICFIDDCMDCDVPLAELTFSRLNFLQRVRTSPEGYAHFTLSGDYYNRALSGWEPFIEPWPCSVSWQQQAASRLHPPRLKLEAKAKPRLDINITSVLIDQYVSTKESWMADYCKDDKDIESAKSEDWMGSSVDPPCFGQSLPLVYLRTRSTASLTNLEHQIYARAEVKTPKRRQPFVPFALRNHTGCTLWFATLT--TTPTRAALSHSGSPGVVPEGNGTFLDDTHNVSEWREV--LTGEEIPFEFE--ARGKLRHRH--THDLRIHQLQVRVNGWEQVSPVSVDKVGTFFRYAAPDKNSSSSTIGSPSSRTNIIHPQVYFSSLPPVRVVFAVTMEGSARKVITVRSALIVRNRLETPMELRLDSPSAPDKPVVLPAIMPGDSFAVPLHLTSWRLQARP-----------KGLGVFFCKAPIH-------WTNV------VKTAEISSSKRECHSMDTEKSRFFRFCVAIKKENYPDYMPSNIFSDSAKQIFRQPGHTIYLLPTVVICNLLPCELDFYVKG------MPI---NGTLKPGKEAALHTADTSQNIELGVSLENFPLCKELLIPPGTQ-NYMVRMRLYDVNRRQLNLTIRIVCRAEGSLKIFISAPYWLINKTGLPLIFRQDN-AKTDAAGQFEEHELARSLSPLLFCYADKEQPNLCTMRIGRGIHPEGM----PGWCQGFSLDGGSG----VRALKVIQQGNRPGLIYNIGIDVKKGRGRYIDTCMVIFAPRYLLDNKSSHKLAFAQREFARGQGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCIWSGGFEVNKNNSFHINMRDTLGKC-FFLRVEITLRGATYRISFSDTDQLPPPFRIDNFSKVPVVFTQHGVAEPRLRTE--VKPMTSLDYAWDEPTLPPFITLTVKGAGSSEINC-NMNDFQDNRQLYYENFIYIAATYTFSGLQEGTGRPVASNKAITCAELVLDVSPKTQRVILKKKEPGKRSQLWRMTGTGMLAHEGSSVPHNPNKPSAARSTEGSAILDIAGLA---AVTDNRYEPLMLRKPDRRRSTTQTWSFREGKLTCGLHGLVVQAKGGLSGLFDGAEVVLGPDTSMELLGPVPPEQQFINQKMRPGSGMLSIRVIPDGPTRALQITDFCHRKSSRSYEVDELPVTEQELQKLKNPDTEQELEVLVRLEGGIGLSLINKVPEELVFASLTGINVHYTQLATSHMLELSIQDVQVDNQLIGTTQPFMLYVTPLSNENEVIETGPAVQVNAVKFPSKSAL-----TNIYKHLMITAQRFTVQIEEKLLLKLLSFFGYDQAESEV--EKYDENLHEKTAEQGGTPIRYYFENLKISIPQIKLSVFTSNKLPLDLKALKSTLGFP-LIRFEDAVINLDPFTRVHPYETKEFIINDILKHFQEELLSQAARILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHAATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFISGLGKGLVGTVTKPVAGALDFASETAQAVRDTATLSGPRTQAQRVRKPRCCTGPQGLLP 4241 HSP 2 Score: 295.819 bits (756), Expect = 3.442e-78 Identity = 298/1256 (23.73%), Postives = 552/1256 (43.95%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAA------LRERARNLVKYVQSY----EDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRK---SRDLLQNAVNIP-----APQQSGWWGYL---------WGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDI--TELLNE--VDFEAVNFFT-RDKIFLRGEFSLEGIKLKLL-KDTEIP------ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-------------NPDYK----NDSFRYVVSSREHSKV-----PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEK---GS---SDIRWWVSLKISAPKIILP-NFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPV-------------TNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPE--------INQNYKSVSI-------SFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLS--------ESIGDP-------------------------------IIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKE-----------FDVSIACKAVSILLIKISSNLASENRFA--VSVGRISLSQMNLLM 3274
M+ L+ ++L+ +GKYV N +T + L KG + LENLPLK L++L E P VK+G IG+VTL+IP+ R W I I +++I P EKE E KK LL ++E KW+ +Q+G++ W S AS+ RI+ N+++++++VH+R+ED T PS FA G+ I S +++ E K + KQ+D++ SIY D C ++ ++ ++ + + E ++ P+ ++KRN + PL H PRI +Q+E + + LSQ QY+ + E ER +RQ +++P+V++ ++ +EWW FA+ + I + + RAR+ V Y Y + LL+ D + E +E + + L LRE+ + ++ + L + + P AP+ G G L WG + +PE ++E L + EQ + ++I TE + D +N +T RD +F + L+ + LL K+ P +++ ++ + ES P+ +S + + L LF+RDL NP + + SF +S + S P G +F + +E NP S + +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ L++ G RW V L ISAP++I P +F+ K P V++DLGR+ ++N D +S +A E E+ +++ TP ++P + S +++ + + V T +Y+ YS+ ++ +VG NW ++ T ++E FN L L+++ + + P ++ N+ L I I++ K+ K C +T + + ++ + +L S + Q+ + V + F ++ + +ES R ++ L + T + +S+ + L+L D G+ D ++ASHK L D P + P +I+ Y+ +S + S ++ + +I ++LD+I N ET+ EL+ F++ F + + Q T F+E +V++ +++LL++ + +A+ ++ ++ I +++N+ M
Sbjct: 1 MLEGLVAWVLNTYLGKYVNNLNTDQLSVALLKGAVELENLPLKKDALKEL-ELPFEVKAGFIGKVTLQIPFYRPHVDPWVISISSLHLIGAPEKIQDFNDEKEKLLERERKKALLQALEEKWKNDRQQKGES------YWYSVTASVVTRIVENIELKIQDVHLRFEDGVTNPSHPFAFGICIKNVSMQNAVNEPVQKL-------MRKKQLDVAEFSIYWDVDCTLLGDLPQMELQEAMARSM-ESRSHHYVLEPVFASALLKRNCSKKPLRSRHSPRIDCDIQLETIPLKLSQLQYRQIMEFLKELERKERQVKFRRWKPKVAISKNCREWWYFALNANLYEIREQRKRCTWDFMLHRARDAVSYTDKYFNKLKGGLLSTD---DKEEMCRIEEEQSFEELKILRELVHDRFHKQEELAESLREPQFDSPGACPGAPEPGGGSGMLQYLQSWFPGWGGWYGQQTPE-----------GNVVEGL---SAEQQEQWIPEEILGTEEFFDPTADASCMNTYTKRDHVFAKLNLQLQRGTVTLLHKEQGTPQMNESAFMQLEFSDVKLLAESLPRRNSSLLSVRLGGLFLRDLATEGTMFPLLVFPNPQKEVGRVSQSFGLQTTSADRSDHYPAADPDGPVFEMLYERNPAHS----HFERRLNVSTRPLNIIYNPQAIKKVADFFYKGKVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTLDRLLVGDFIEESKRWTVRLDISAPQVIFPDDFKFKN--PVLVVVDLGRMLLTNTQDNSRRKSRDGSASE----ETQFSDDEYKTPLATPPNTPPPESSSSNGEKTPPFSGVEFSEEQLQAHLMSTKMYERYSLSFMDLQIMVGRVKDNWKHVQDIDVGPTH------VVEKFNVHLQLERRLIYTSDPKYPGAVLSGNLPDLKIHINEDKISALKNCFALLTTPEMKTSDTQIKEKIFPQEEQRGSLQDSVMNLTQSIVLLEQHTREVLVESQLLLAEFKVNCMQLGVESNGRYISVLKVFGTNAHFVKRPYDAEVSLTVHGLLLVDTMQTYGADFDLLMASHKNLSFDIPTGSLRDSRAQSPVSGPNVAHLTDGATLNDRSATSVSLDKILTKEQESLIKLEYQFVSSECPSMNLDSTLQVISLQVNNLDIILNPETIVELIGFLQKSFPKEKDDLSPQPLMTDFERSFREQGTYQSTYEQNTEVAVEIHRLNLLLLR-TVGMANREKYGRKIATASIGGTKVNVSM 1207 HSP 3 Score: 172.94 bits (437), Expect = 5.625e-41 Identity = 147/475 (30.95%), Postives = 236/475 (49.68%), Query Frame = 2
Query: 5465 CLLDEMKIDLHEMDVYSAIRITDSILK-----DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLM--SRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRL-NVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSL--ATDDENRFDQHVAFYIQSKPQSRSISFQ---PVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT 6844
CLLD + +DL +MD+++A R K GDL F +R L+E C L L V RNL+ ISHT PD I+G LSSV S+ + +Y+ IRG+L +NLGE + ++ Y Q DP +HT L+G Y M + + +V L L R +G +L R +F + KL YESFS+ +K+I LV + D RY+ + + NVF I P KN+ ++ I Q+ + + S D + F V LN ++ +IFDW+ + +++ T P I T +R + + +S ++ + PV +H E+ +NV + F ++E+ S T+A+IL +YK R +R PFS S+ + SC + ++ LSI++PV+I ++ L D + ++ + ++I + A IRLS++D+QL I +S Q
Sbjct: 2151 CLLDCVVVDLQDMDIFAAERHPREYSKAPEDSSGDLIFPSYFVRQTGGSLLTEPCRLKLQVERNLDKEISHTVPDISIHGNLSSVHCSLDLYKYKLIRGLLENNLGEPIEEFMRPYDLQ----DPRIHTVLSGEVYTCMCFLIDMVNVSLELKDPKRKEGAG-----SLARFDFKKCKLLYESFSNQTKSINLVSHSMMAFDTRYAGQKTSPGMTNVFSCIFQPAKNSSTTQGSI---QIELHFRSTKDSSCFTVVLNNLRVFLIFDWLLLVHDFLHT-PSDIKKQNHVTPSRHRNSSSESAIVPKTVKSGVVTKRSSLPVSNERHL-----------EVKVNVTGTEFVVIEDVSCFDTNAIILKGTTVLTYKPRFVDR--PFSGSLFGIEVFSCRLGNEHDTALSIVDPVQIQMELVGN-SSYQNSSGLMDAFNSEDFPPV-LEIQLQALDIRLSYNDVQLFLAIAKSIPEQA 2597 HSP 4 Score: 157.532 bits (397), Expect = 2.056e-36 Identity = 149/534 (27.90%), Postives = 262/534 (49.06%), Query Frame = 2
Query: 3980 KAVYEQVLKSMDNLVY-DDEDEIPK-------------PFIEFEDENMQEDQNPDPSENEIPIVLQSN-----------------ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSS---SNIGQENLQEKS------VSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNS-VDIQNCAAFELKLPKNTV-KSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVK----DLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF 5443
K VYEQVL+++DNLVY +D ++ P P + +++ +N S + + Q + IS+++++L D ++ ++ L Q +EF + + I++A+ + + +L+ K L+ S + S++ Q+ +S V PD+ L S + P K++K + + + + + PK V KS + L I I L+DKK FS+ + ++R +D DFN L I+++ WV++LDFFG+GS D ++ +L K+ P + + +++ +NTKL++ + SL+L T + +N VS + L+ +G++ L GS+ L DL+ G Y +R F S ++ ++ F FKYG PDP L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V AQR +R+ + I AP+LL+P S+ S+++++ +LGK + N+F F
Sbjct: 1539 KHVYEQVLQTLDNLVYSEDLNKYPASATSSPCPDSPLPPLSTCGESSVERKENGLFSHSSLSNTSQKSLSVKEVKSFTQIQATFCISELQVQLSGDLTLGAQG-LVSLKFQDFEVEFSKDHPQTLSIQIALHSLLMEDLLEKNPDSKYKNLMVSRGAPKPSSLAQKEYLSQSCPSVSNVEYPDMPRSLPSHMEEAPNVFQLYQRPTSASRKKQKEVQDKDYPLTPPPSPTVDEPKILVGKSKFDDSLVHINIFLVDKKHPEFSSSYNRVNRSIDVDFNCLDVLITLQTWVVILDFFGIGST-ADNHAMRLPPEGILHNVKLEP--HASMESGLQDPVNTKLDLKVHSLSLVLNKTTSELAKAN---VSKLVAHLEMI--EGDLALQGSIGSLSLSDLTCHGEFYRER-FTTSGEEALI-FQTFKYGRPDPLLRREHDIRVSLRMA-SVQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAIEGQTVRDQAQRCSRVLLDIEAGAPVLLIPESSRSNNLIVANLGKLKVKNKFLF 2060
BLAST of Vacuolar protein sorting 13 homolog D vs. UniProt/SwissProt
Match: sp|A1Z713|VPS13_DROME (Vacuolar protein sorting-associated protein 13 OS=Drosophila melanogaster OX=7227 GN=Vps13 PE=1 SV=2) HSP 1 Score: 179.874 bits (455), Expect = 3.497e-43 Identity = 204/878 (23.23%), Postives = 393/878 (44.76%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLN-------EMPKSDCIVNPISLHGIMKRNINPL---PMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRP-RVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDT-----------EIP-ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIK---RILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVGNWNE-LESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELT 2545
++ ++ +L++V+G Y+EN +K G++ GD+ L+NL ++ L++L + P+ + G +G++ L+IP+ SQ + IED+Y++ PN+N + EKE + E +KK LD++E +K++ D K + + L +I+ NLQ+++ NVH+RYED +T S F+ G+ + ++D +WE K + + + K +LS +S YL+ ++ + N KS L ++ P + ++ PIS + +K N+NP P +P+I L L+ME++ + L+ Q+ + L D R + KYRP + ++WW FAIT R + ++ Y Q Y++ L+K S + E LE++L++ LL +R+ I ++ + + + ++ +T+ + L + + TS+ EK+ R E D+ E +EA+ R F L +++ L KD E+P ++ +N RP + I G+R + V L +ND +V S+ + E+F FETNPL C +K R L I+ + TI+ LI Q + + AA I N K R++ ++ +++K + + +++PN + H+ G N S ++ + +P ES+ L++L A + + + N YD +++ +++V LV E ++ L++ S ++ ++ P + + CV++ + PNI ++ ++ +L+++S+ ++ L
Sbjct: 1 MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIRENALDEL-DLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEKEAKYEMDLKKAALDALEAARKKELEM--DQPKADA----GFAEKLTAQIVNNLQVQITNVHLRYEDTTTTGSP-FSFGISLHELELYTTDCDWE-KCYMAQQASQVFKIANLSCLSAYLNCGGQLYA-----NNKSDLSQQFKTNIACKETKPNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKIDLTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAITSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETELDVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGW----GGGAKKDDQTTSQKLVEKFEAAMTSEEKEKMY-RAIGYQENAKPTDLPE-----SYEAI----------RMNFKLIALEVGLYKDERNSSAATKDFHELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLT---RNDYTPLLVESKITDEFNLLEVF---FETNPLDKLCDQRVKVVARPLQITYDAPTIL---ALINAFQTPGDVTLSKFEDAASTKISNFKERSATGMQYMIDKKAV---------LDVDILLMPNILVVPHK--------GVYDAGNVSLLVVSMGQVHLSSQPRR-ESN---------------KLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPWQNALAEANST--EMHVLRPVSLKV-TAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFLA 799 HSP 2 Score: 97.8265 bits (242), Expect = 2.804e-18 Identity = 98/399 (24.56%), Postives = 168/399 (42.11%), Query Frame = 2
Query: 10235 LLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLK-------FQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMF---LSPSKEAELLEEDFSSLQ------HQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFPSLM-----SFTNAQIII---DVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ-----------------DVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSS---KEGQPPR 11299
L V I +IQVDNQ +D + V+ P S + SLK QR + + V F + R+ I ++ L +MF +S A+ +D S++ + H E +K + NL L I +S A SD K + SL+ + T+ ++ ++R + F + + I HY Q Q + +D LGNP G + + G++ L+ + V ++ H G + +KI + + ++ +T D +Y++KR++ +N + + K L G G+ V T+ G +D G G F G KG +G+V +P G++D A+GS + + A +S K +PPR
Sbjct: 2783 LHVKINRIQVDNQFLDPIFPVVLA---PIPPPKSVASTTSLKPFIECSMVQRIMPNS-TVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVESIELPLSAFFEEHSLEEQKSFYDNLHLGPLKIHVSFSMA---GSDTKALPGFLGSLVQGVGVTLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDEDYQKKRRQGIQNK--PKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGARDNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPR 3172 HSP 3 Score: 64.3142 bits (155), Expect = 3.324e-8 Identity = 88/364 (24.18%), Postives = 159/364 (43.68%), Query Frame = 2
Query: 8471 YDDQNRL-LELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTI-TLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNLLVF 9556
YD + ++ ++L VK ++R G + + + S +W++N+T + L Y +S T H PE S P F F +K F + + ++ N + WSEK+ L+ G+ + A+ N Y +G+ L + TK + Y++ N+ F + + + D P + L + + WPR+D L V ++D P + F +C L + ++ + V + T +G Y + FTD +P L N T Q+ ++ E + +L KS I YA D+PT P ++ + G + D+ + G G+ + D + + SF L +L+F
Sbjct: 2328 YDSEMKVDMDLYVKTENR-HGSLMLTLFSPFWMINKTGMMLTY-KSETTSVEVLYHPPE-----YSGPILFTFRDK----LFFDKKKASIRIDNGQ-----WSEKIPLDVAGSVGEVICFAN------NQKYPVGVHNHLTQNSL--TKQITFIPFYIVCNKCHFDIELQEQSRPAD---PWLHLEPNEMEPLWPRNDTKNNLVV-RVDGKITP----AFDFTEVICT-----LLKLEDSKYGGINVDVQTTEGGVY-ITFTDYKPADAPGLLINHTGKQIVYH----EKGTKNEHILNAKSTIMYAWDDPTGPKML---VFGTNKEETDLKRDGIGEVIMQDGGKVLWV--SFLDGLQRVLLF 2644
BLAST of Vacuolar protein sorting 13 homolog D vs. UniProt/SwissProt
Match: sp|Q54LB8|VP13A_DICDI (Putative vacuolar protein sorting-associated protein 13A OS=Dictyostelium discoideum OX=44689 GN=vps13A PE=2 SV=1) HSP 1 Score: 154.066 bits (388), Expect = 2.602e-35 Identity = 182/755 (24.11%), Postives = 344/755 (45.56%), Query Frame = 2
Query: 35 LITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDL-FEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS-NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDND--KVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPK---------------SDCIVNPISLHGIMKRNINPLPMNHQ--PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVF---AITEHTR-----HIFKGAALRERARNLVKYVQSYE------DYL--LNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKS-RDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR---DKIFLRGEFSLEGIKLKLLKDTEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVP--GGEIFALAFETNPLPSTCPP--NIKRILAISSEPLTIVHCPLLIGNIQEYF---SRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTA 2155
L++ +L RV+G+YV+N + +K G+F G ++L+NL LK L +L PI VK G +G++ L++P+ +S+ I I+ IY +A P + N+K EKE + + + +W K I+ +N+ ++ + S+ L +I+ NLQI + VH+R+E+ + + +++A G+ +D S +S+D W +++ + K + + Y+D +N S+ KL+E K S I+ PIS +K NIN + + P+I + M Q+ +LS GQY+ +++ + + R ++K+RP V V E+PK WW + I E R ++ A R RAR + Y+ ++ D+L LNK E E N LE L + ++ R +A I +++ ++ ++ Q+ G++ ++ + E EK P+ +SE E+D EL +++ V + D + + G F ++G ++L++ ++ +E L + FE R + I L ++ + K F +++S VP G FA + T PP N+ + ++ +PL I+ LI + ++F + NI A H+ + K + ++ ++ +++ I AP I +P + + +++DLG+L V + + + S K+
Sbjct: 6 LVSDVLSRVLGEYVKNLNKDQLKIGIFGGSVQLQNLELKEDALANLPINLPITVKKGFLGKLDLKVPWKDLKSKPVIINIDCIYALAVPQTQNYKYDEKE-EAKKQAELKKKKLENYEWIKSIKDAEENEISSLQSEKSDSFTDRLVTKIIDNLQIVINKVHIRFENKNDI-GKLYALGITLDKLSIQSTDEFWIPSFVDSSKSKFIKKLALMDSLGFYID-----------DNADSLQNLKLDEFTKAFTSLIPSSASFSLLSKYIIKPISSQ--LKLNINKSDIIEKSIPKILVECVMSQITCTLSSGQYQNILSILNFTNEYLRDIKYLKFRPIVKVSENPKLWWRYVGQVIVEQIREKRFSRSWEFIAQRRRAR--LAYIPLFKRNIPKVDWLQPLNKH---ELEQLNQLEERLSFEDIVYFRSLAYAEIKKETEKNKIRKQFLDSKRQERGFFQNIFNTKKDED------------------EKAAPKIQLSSE---ERD--ELYKTIEYNDVVSASEEPPDWVKIIGNFEIKGFAIQLVEANQV-FIEALYTGLSLKFEQRNEGIKVMAGIHLFEVY----DQFTKRTHFPKIIAS-----VPTKGTGTFASV-----IVDTRPPDKNLDLSVELNMDPLNIIVTKPLIMKVVDFFHDPNLDITNISNRAGAHLEDIKEQAKMQLQDAIDNHKV---LGLAVNIHAPVIFIPE-DVINERSNILIVDLGKLVVRSDTSAFVKGSNKLAG 698 HSP 2 Score: 75.485 bits (184), Expect = 1.317e-11 Identity = 63/243 (25.93%), Postives = 102/243 (41.98%), Query Frame = 2
Query: 10670 SLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSD-----------------QDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPY 11347
SL A I + H F + + I I Y Q Q I GSVDFLGNP+G + TG+ + + + + VK+ G ++ +K+ + ++ ++ D +Y ++R+ AR + G L GI GI + T+ +G K G +G G +G+VGV KP ++DLAT + + ++ + Q R PRC S L P+
Sbjct: 3000 SLTRLDRAPITLQGLLMEHPFTSRSTIIDKIKTSYIQQALRQFYNILGSVDFLGNPVGLFRNFGTGVHDFFVEPAQGLVKSPADFTKGLAKGTSSFVKNSVFGTFNTLSKLTGTLGTGVATLSFDEKYLQERK--LHQARKPSHVGEGLAMGGIGLGRGILQGITGIVTKPVEGAKKGGFAGFAKGLAQGVVGVAVKPTTAVIDLATKTTEGIKNT--TNLQSQAERVRPPRCFSHDNVLRPF 3238
BLAST of Vacuolar protein sorting 13 homolog D vs. UniProt/SwissProt
Match: sp|O42926|VP13B_SCHPO (Vacuolar protein sorting-associated protein 13b OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vps13b PE=2 SV=1) HSP 1 Score: 149.443 bits (376), Expect = 6.275e-34 Identity = 202/867 (23.30%), Postives = 369/867 (42.56%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMMKKLLLDSIEVKWRK----KIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYED---NSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMP--KSDCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRV---SVMESPKEW--WVFAITEH---TRHIFKG----AALRERARNLVK-YVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWG--RRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEA-VNFFTRDKIFLRGEFSLEGIKLKLLKDTEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKAR--TSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVGNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLEL 2542
M+ L+ L+R++G+Y+ENF +K ++ GD+ L NL LK + L E PI V+ GL+ +TL+IP++ +++ I I I +A N K Q+E ++L E K R+ + Q G + ++ SL R++ N+QI +R++H+R+E ++ +P ++ GLL+ FS +S + EW S T + TE + K L G IY D + + + + + + Q + + + D I+ P++ G+ K IN LP PR + ++SL+ Q ++L+ E + + +R R+ +++P ++ ++F T H +H + A E+ RN +K Y + + L+ D + E + LE +L++ L R +A + I + P+Q GW ++W R + + + E+ D R + GL I E + D ++ + F G+FSL LK K + LE+ N F+ + S+ +G ++ D N Y + +S + + IF L+FE S+ N L I+ L + P L+ ++ + S N + N TS++++ + E + + V+ + P +I P E + +D G + +++ + + D+ ES+ ESE +L +L IYD Y + LE+ L+G NEL++E +K N + LM N + ++ + V + Q P I +E N+ L +SD + +
Sbjct: 1 MLEGLLANFLNRLLGEYIENFDATQLKVAVWNGDVTLRNLQLKRSAFRKL-ELPISVQYGLVEELTLKIPWSSLKNKPVEIYIVGIRALASMEENVKS-----QSEVDPHQVL----ESKRRQMQLWEASQIGKAETAYDPKTQTFTESLITRMIDNIQINIRDIHIRFEHLPVSNVVPGG-YSFGLLLSEFSIESCNEEWTS-TFVETESQTIYKLCSLRGFGIYSDETAECIDKTDINELMNTFQALITDFQSREKDYIIAPVT--GMAKVTINKLPTPEIPRFLSQISFRGFDVSLNDSQISCGMSLAHELQDV---MSKLAFRKRIVGNVSLDTPLDYLRFIFQKTLHDIQQKHYARSWPAIKAFCEKRRNYIKLYKKKFLAVQLSADESKELDV---LELNLDISQLKLFRSLAYQEIKNE-------GFEPQPPKQQGWGSWMWNSFRGASDENGEVTDDQ---------------RKTVIDAIGLNDSIIESAHVGDLQSNTSLFDVRLEVPSGKFSL----LKYPKKENVISLEL--LNFFSQFKCNKKDYSFVANLGSLKMYNDDRNFLYPRE------TSNKEIETTSPSIFTLSFER----SSSDDNDTDTLGINLRALEFFYDPNLLLRVKGFQSSLFANDNGRKLVRLANDAVTDFTSQTVQNLQEYLNEKRKLNVNFQFQTPLLIFPE-DCNNPESFSLFVDAGFISITSQNVETNDK------------ESNSESE-----------TLYHL-----------------IYDRYRVSLESARLLLGPLNELKNESNK---INEEYYLMNELNTEIFIQAQRV--PTPQYPRIKVEGNMPCLSFILSDAQFRI 763 HSP 2 Score: 93.2041 bits (230), Expect = 6.660e-17 Identity = 105/461 (22.78%), Postives = 201/461 (43.60%), Query Frame = 2
Query: 10127 VEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDE------GRKIYIGNLKLLTGVIS---LSVITAESLPSDLKQMKKLFPSLMSFTNAQI--IIDVYDR----NHVF---ETGNL--IAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSD-------QDVYNMVKHFA-----------HGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYK---------DEGPSGLFI-GALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPYQ 11350
++GFG+ LI+ EE YA N + + + + +G +Q+DNQ +D+ Y ++ +E K + P Q L+F +++ + + + + + +E++++L +Q+ PS + + +D +SL + D+ +Y N+ T + L++ S SD +K P+L T I + +++D N + G L +A +A HY+ Q+ Q KI G DFLGNP+G +++ +G+ ++ + QD + FA +G+ DS +KI + S+ +S +TMDP+Y+ R+ + + K YG+ G S + G+K +G F+ G KG++G+ TKP G+LD+ + + R + + PR + ++P++
Sbjct: 2448 LKGFGISLIDKKYEEFAYATLKNFTFRFDDSKDLNTFGMSLGWLQIDNQMLDSVYPIALFPTMITQEVKQDDPQLLQ----LRFSVLKDSSFNILYIKYASLLLQELSLEVEDRLVLTLLQLLY-PSSD---VSKDSASLSKNAFADKFEIPDLDADVYRSNVFFETLHLQPTRLNISFETSYESDQPAVKSSNPTLDFMTGILISTLGNIHDAPVQLNSILLENARGTLSEMANRVASHYKQQVGYQIYKIAGRADFLGNPVGLFNNVASGVFDMFYEPYQGFLLQDSQSFGDSFARGTSSFMRKTIYGVSDSVSKITGTISKGLSTMTMDPKYQNSRR------------RFRSRNRPKEAVYGVTAGANSFYDSMSSGFKGLKKPFTDPKNNSAGKFLKGFGKGMLGLATKPAIGLLDMTSNVSEGIRNSTDVRTNPEIDKVRVPRYVEFGGLIVPFK 2888
BLAST of Vacuolar protein sorting 13 homolog D vs. TrEMBL
Match: A0A4W3GGU7 (UBA domain-containing protein OS=Callorhinchus milii OX=7868 PE=4 SV=1) HSP 1 Score: 1122.07 bits (2901), Expect = 0.000e+0 Identity = 1120/4301 (26.04%), Postives = 1911/4301 (44.43%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMM----KKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR-------ERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLE-LDCLLQLREVAMEHIGRKSRDLLQNAVNIPA----------------------------PQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLEGIKLKLLKDT--------EIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSR--------EHSKVPGGE---------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRP-LAELSLTKTQFKL----------SRYSSM----------------TIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLS-------ESIGDPII-------------------EFTYKISQKSGSSDIQ--------RQINLIFSDLDVIANQETLTELLSFMRLLFS------------NDSLQIFDQKPETPNTTEFKEFDVSIACKAVSILLIKISSNLASENRFAVSVGRISLSQMNLLMHKNGTALNSTLRIR-SIN-----VEDISILDSLHKNILTLTTGSNETKMEIS------------ENEDVINISV---IQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAA------VARTA--------------------------ILSSEESTRIGFLNSVKLHLRMLSPILALPRHKTSNQIIL------------------------VNLGDIEITS----------------------DTQSLQVAFSDAIVISKELPIENGGYDIGICTDNNAKFPLVN---KIDLMLKFSKLHDP------PKILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDE---------DEIPKPFI--------EFEDENMQEDQN--------PDPSENEIPI-------VLQSN--ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIPPPSP-----------FYKY---SKRKKSLSNSVDIQNCAAFELKLPKNTVKSTEPN---KLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYD-----PKDVKDLL---DVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFE----------------------------------------NNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDG-----DLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRL--NVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYG----IVSLATDDENRFDQHVAFYIQSKPQSRSISFQPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS-------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQN--QTIPPANLAYQTGYLRHRWSSLNFDNVNT--------------------------SKPSQPNLTALQKLSK--------FVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--------------QERNVAFCDC---------RKRHNNLFVPFLIVNELGCPIEYR---------KLSLDNPPMYNEIWNANKCGDEN---KWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLK---PNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLF-YSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP--LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQN-VSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFES-DLDNL------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDP-KQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCK---NAAVFGGFETFKEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSST--------FSNIEMHFSVEG-FGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY-----EENKSE----SPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG---RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFP-SLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYK-DEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFRE 11263
M+ L ++L+ IGKYV N +T + L +G + LENLPLK L++L + P VK+G IG++TL+IP+ R + W I I +++I + KL E + + E++ K+ +L+++E +W+ + +Q+G++ W S ASL RI+ N+Q++V++VH+R+ED+ T P FA G I + S ++++ E E + K++ + SIY D C+++ ++ ++ + L E ++ P+ ++KRN + L PRI +++E + + LSQ QY+ V E ER +RQ K+RP+V+V E+ +EWW+FA+ + I + LR +RAR+ V Y + Y L E E D + L+ L LRE+ EH+ + +D L +V P P GW+GY ++ SP D + +I + + D ++N TR D +F + F L+ + LL+ E +E+ + +G ES P+ S + L LF+RDL + F +VS + E + P +F + +E NP+ S N +R L +S+ PL I++ P I + ++F + + + AA + K +T I++ +++ D RW + L ISAP++I P+ K+ + V++DLGR+ ++N ++ KV A ++ + D S+D + + Q E +Y++YS+ ++ +VG NW ++++ P ++ E FN +L L+++ + P+ ++ + L I +++ K+ +++NF++ A++ + Q+ + E Q K + L +I LE +R L E L +F++ RY S+ T +++ + L+L D GS D ++ASHK L D P +S P++ + K+ + SSD + ++L ++LD+I N ET+ EL+ F++ F ND+ F K E+ +T + +V++ +++LL++ + L+S + + S+ NGT +N ++ R +N ++ + + KN ++ G+ + +S E+E ++ ++ + E + F ++ + + Y HSP+FL + + E + F + + A+ V +TA + S ES R + + L++ + SP++A+PR S+++++ V + DI++ S + S ++ + + +++ E+ I + +F L ++D L F L P K+ IE +F + L ++F+K VYEQVL+++DNL Y +E P P +F D + ++ P + PI +Q+N I +++++L D ++ ++ L+ Q +EF + + I++A+ + + +L+ K L+ S + +E S SCP S SN +P P FY+ + R+K D + N L I + L+D+K F++ I+R VD DFN L I+++ WV++LDFFG+GS + P + L + S VLP+ +E NTKL++ + SL+L +T+ I +N++ ++ DL DG+ L GSL L DL+P G +Y +R F S ++ ++ F ++KYG D L RE D+R+ +++ + Y+HTQRF E+ F Q+F Q QD L R S G+ V AQR++RI + I AP+LL+P S+ S+ +++ +LGK + N+F F +++ CLLD + +DL +MD+++A R + D+ F +R ++ L E+C L L V RNL+ ISH PD I+G LSSV S+ + +Y+ IRG++ +NLGE + ++ + Q DP +HT LTG Y +M + + +V L L+ + +L R +F +SKL YESFS+++K++ LV + D R N L NVF I+ K++ + Q+ + Y S D + F V LN ++ +IFDW+ + ++++ T+ G S A + N + + PQ+ +I ++ +++ E+ +NV + F ++E+ S L T+A+IL +YK R +RP FS S+ + SC + ++ LSII+PV + I+ + G P+ LD D L +I S IRLS++D+QL I++S P A + + ++ L D + K ++P AL + V + + E + +CLIDDC+D DVPL ++ F+ + +G + G F LS DYYN GW P +EPW + W K L ++ L+ +I+S L++ K T W ++ Q R + D + FVP+ + N GC + + LS P Y + D + +W V F+ AR+K+R+ +H H++ +++ GW + PV+VDK+G+F+R+ D + V + I R+V + +GS + I +RS L + N L +P+E + + S+ VVL P + IPL + +Q RP ++ F +PI W V+ G+ ++ C S+ + +K I++ N+P L MV PGH + + L N LPC++ + +K + S + + L T Q+ + L++FP C LLI ++ V +RLYD + RLL L +++ R G + ++IS+ YW++N+T LPL++ Q + AAG E+E A S P F+F++K +R+G + P W ++ S + +RA V Q NRP L+Y+IG++IR GRG++ T +V RYLLDN+SS L F Q Q I LP + + FHWPR+D DQLL V+ +D ++ SGGF+I NSFH+ +R + N L V ITL+GA YR+ F D++ LP P++++N + + + F Q+ V+E R + + P + + YA DEP L P I L ++G S D+N + L+Y+NF+YIA ++F D N LV D+ +V L +K RSQLW + + L HEGS PH+P K R +++ L+LD+ LA +S ++L K D RR Q W F +G + GM + A V G E P+ + F RPGSG + + V+ DGPTRV+++ D + S L D T N+E+ ++G G+ LI+ +PEEL++ +D+ + + L + I IQ+DNQ + ++ EN+++ P+ NA + + L +F + + + + + +EEK+LLK + F + ++ + D ++L+H H D+G ++ Y NLKL I +SV T+ LP+DLK +K + L+ F +A I +D Y R H +ET I I H++ +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + A SG + + AG + LA+GI GG SV T +G K D G G F G KG+VG VTKPV G LD A+ +A + RE
Sbjct: 1 MLEGLAAWVLNTYIGKYVNNLNTDQLSIALLRGAVELENLPLKRDALKEL-DLPFEVKAGFIGKITLQIPFYRPHVEPWVISISRLHLIG---AQAKLTEFDEEKESLAGCERKRAVLEALEARWKSEQQQKGES------YWYSVTASLVTRIIENVQLKVQDVHLRFEDDVTSPEHPFAFGFCIKSVSMQNAEDE-------PVEKSMRKKRLQIEDFSIYWDVDCELLGDLPPSEVQEAMDCCL-ENRNHKYVLEPVCASALLKRNCSKESLRSRSTPRIECSVKLETIPLKLSQVQYQQLVTFLKEMERKERQLRLRKWRPKVAVAENSREWWIFALNANLAEI-REQRLRSNWSFVLQRARDAVFYTEKYYQKLKGGLITPEEEKELERIEDEQTLEELKILREIVHEHL--RKQDELAESVREPVSDPPTACPRTDPVAGRRSGVIKYLQSWFPGWGGWYGY--HHNPQQLSPGESDFVCFILAADEIFDPM----------------------ADTSSMNTITRRDHVFAKLNFLLQSGTVSLLRHVKETTLDVKEAAFVELEFSGVKIGVESSPRTESTLFSVQLGGLFLRDLA--TQGTLFPVLVSPKPVISLLHTEPNNNPSKTKKIIKLMPPVFEMIYEKNPVRS----NYERRLEVSTRPLNIIYNPQAIKKVADFFYKGKAHSAGFAYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLEGEFIEKDKRWLMKLDISAPQVIFPD-DFKSPDSVLVVMDLGRILLTNLQEES-----KVKAKTRLSLKEDEFSDDEYQTPLATPPNTPPPEPTEQLQ---ERFLNNKLYEKYSLSFMDMQIMVGRVKDNWKH-----AQDSEVGPTHVV-EKFNVLLQLERRLMYTSDPNRPSAMLSGTLPDLKIHVNEDKIV-------ALSNFLSRMTTAETPQTWAEPQSLRREKIFTKEGRQG-KLLDSRMSLTQSVIALEQHTREMLVESKLLLAEFRVNYMQLGVESCGRYISVLKVFGINSNFVKRPYDTEVTLTVHGLLLVDTVQTYGSDFDLLMASHKNLSFDVPTGSLRDSGPQSPASPLLKSSQLPGEQVPSVFFAQDQDVLIKLEYQFVSSDCPSMNLDSSLQVMSLQVNNLDIILNPETMVELIGFLQKSFPKGKEDASPQPLLNDAEMDFGDK-ESIQSTYDQNTEVAVEIHRLNVLLLR-TVRLSSGEKLGRKIATASI---------NGTKVNVSMGSRFEVNGSLGCLQLVDLTQDDVKNHFVVSIGNTQAYETVSDFGFFGPVFMRPESEGNLSEALSFKLLEKSQEEFSLNLHMASLHYNHSPKFLKELSLSMDELEDNFQNMLKSAASKVSTVLVNKTAEYSEMVMLFELPRRSREMFTFDYGEELTSGMESPRQKPIKLI-LNINIESPVVAIPRRPGSSELLVGHLGRTSIQNFVTGEDDSERDRLQVEIKDIKLYSLNSSTLGGPRGSRGDGSESSRPGSGSASISSQEEVHFTRQDFFESVHKAFHILNNTTIQFILEKIPLELDQHLSFPSLSSPDSYETTSKMKIEGKFVNPLKVTFAKHVYEQVLQTLDNLTYSEELSKMPHSSAAATPDPLSTSSALRSPKFSDLQREWKESGLFGRVSYPSTPVDSFPIPESRSFTQIQANFCIPELQVQLCGDLTLGAQG-LISLVFQDFEVEFSKDQPHTLSIQIALRSLLMEDLLEKNPNSKHKRLMVSRGAPKASSFTHKEYLSQSCP--------STSNARYPEMPRSLPAHVEEVQNVFQFYQRPTSTTRRKPKEEQDDYPLTPPPSPTSRSSRAPGNPTNFDDSLVHINMFLVDRKHPEFASRYGRINRNVDVDFNCLDVLITLQTWVMILDFFGIGSTANNHAVKLPAEELHQLSKSEASHVLPAPG-------QEPSNTKLDLKVHSLSLVLNKKTNEIAKANVSKLAA-HLDL----IDGDFALQGSLGSLSLSDLTPHGELYRER-FTTSGKEALV-FDIYKYGKADAKLKRECDVRVSLQMA-SVQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAASEGQAVRDRAQRASRILLDIEAGAPVLLIPESSKSNQLIVANLGKLKVKNQFLFVRSPGTFSLRDKVQYNHHVNRSVFGSVLPFLLHSSDTLDICSSEEHVCLLDCIVVDLQDMDIFTAERRSREKAAGAECSISDIFFPSYLVRQLDGNVLKERCWLKLQVERNLDKEISHAVPDVSIHGSLSSVHCSLDLSKYKLIRGLIQNNLGEPIEEFMRPFDLQ----DPSIHTVLTGEVYTSMSFLIDMMNVSLELLESKS--KSGNGSSLARFDFKKSKLLYESFSNATKSVNLVSHSMMAHDTRSPGQMMNPDLKPNVFQCILDASKSSCNR----GSLQMELHYRSTRDASCFTVVLNNLRVFVIFDWLMLVRDFLQTSSTGKKPDTSSPAREHRNNCSEILI------PQTVKSG---IITKRSSVPAPSDKY--LEVKINVTGTEFVVVEDVSCLDTNAVILKATTVLTYKPRLVDRP--FSGSLGGIEVFSCRLGNEQETALSIIDPVNVQIE-------LMGNPTRQESSGLLDAFSSDDFPPVL--EIQFSTLDIRLSYNDVQLFLAILKSIPKDVSVCPGARFSLVLSPVSFLFTELQLDRLQDLGFSLEDCRKALLTCKGQLNHAATWLFKNAEPMSAALASAGESRGANSGMLVSGVEV----KAESICICLIDDCMDCDVPLAELTFSRLYFLQRVGTNTGGSAHFTLSGDYYNRELSGWEPFVEPWTCFLSWQQQVASRLHPPRLKLDLQAKQRLDINITSVLIEQCKRTKASWIADYCKQVEKDSGSAGRLQWRTGVYMDPLMLGQTESKMPKRRQPFVPYALRNHTGCTMWFATLTTSPTRVALSHSRSPGYIPETSGTVLDDMHNVSQWQEVLPGEEVPFKFE----AREKLRHRH--THELKIHQLLVRVHGWEQVSPVSVDKVGVFFRYAAPDRNSQSSTVGSPISKTNIIHPHVYFSALPPVRVVFSVTLEGSARKVITVRSALIVKNKLEVPME-LRLDSPSAPDKPVVLPAVLPGDSLAIPLHLTSWRLQTRP-----------KALGLFFCKAPIH-------WTNVSKVGEVNSSKRECHSA----DCDDMSHKQSFCVAIKKENYPDYLPLNMVSGNAMQVFRQPGHTVYLLPTMVLTNLLPCELCYYIKGTSISGPLKRGKDAVL---------HTADTSQNIELGITLENFPTCKELLIPA-GTANYYVRMRLYDTKKRLLNLSIRIVCRAEGSLKMLISAPYWLLNKTGLPLIFRQDN-AKADAAGQFEEHELARSLSPLLFSFADKEQPNMCTMRVGKGIHPDGI----PGWCQEFSPDGGSG----VRAVKVIQHGNRPGLIYSIGIDIRKGRGRYRETNIVTFAPRYLLDNKSSHKLAFAQREFARGQGTQNPEGYISTLPGASVVFHWPRNDYDQLLCVRLMDVSN---CTWSGGFEISKNNSFHINMRDTHGNC-HFLHVEITLKGATYRIAFGDSEQLPPPFRIDNFSEVPIIFNQHGVAEP--RLKTEVKPATSLDYAWDEPVLQPFITLTVKGAGSSEITCDMNDFKESKQLFYENFIYIAASHTFSGYDSGNKSISRAELVLDIVSKTGRVILKKKEPGKRSQLWRMTVSGTLCHEGSSPPHNPNKPSSGRHSNSGLVLDIASLAGVTDNS---CEPLVLKKPDKRRGSTQTWCFRDGQLACGMPGMVVQVRRGSGLYDGAEVVLGPEPSAGLLEPVPAEQQFSNQKLRPGSGVLALRVIPDGPTRVLQITDLQQRR--SNRSLTGAEDCLTPEISEVRKMKNLEVLVKLDGGIGLSLISKVPEELVFTSLTVIDIHYTQTVGSQMLELSIQDIQIDNQLLGTTQPILLSLTPNSVENETDYVEAGPAVQINAMQIPSKSPL-----TELFKHLMITVRKLSVQIEEKLLLKLLSFFGLGQLDQDVEKPDENALEH--HSDQGGTLKRYYFENLKLSMPQIKMSVFTSNKLPADLKALKSMLGIRLIRFEDAVIHLDPYTRVHPYETQEFIISDILKHFKEELMSQATRILGSVDFLGNPMGLLNDVSEGVTGLIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDTRHQAERESIRYQAVTSGEH---LVAGIQGLAHGILGGFTSVITSTVEGVKTDGGVGGFFSGLGKGLVGTVTKPVAGALDFASETAQAVRE 4085
BLAST of Vacuolar protein sorting 13 homolog D vs. TrEMBL
Match: A0A1A8UK89 (Vacuolar protein sorting 13D OS=Nothobranchius furzeri OX=105023 GN=VPS13D PE=4 SV=1) HSP 1 Score: 1121.3 bits (2899), Expect = 0.000e+0 Identity = 1142/4311 (26.49%), Postives = 1958/4311 (45.42%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMM--KKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLE---LDCLLQLREVAMEHIGRKSRDLLQN-------------AVNIPAPQQS-------GW---WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSL---------EGIKLKLLKDTEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSR-------------EHSKVPGG------EIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS-LENLSMATDCQ---------ASKETKPVTN--IYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTN----------------FMNNYRNADSSSVKYKKQTRMDNLNKSPEI-NQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSES-IGDPII----EFTYKISQKSGSSD-------------------IQRQINLIFSD-------------------LDVIANQETLTELLSFMRLLFSND-----------SLQIFDQKPETP-NTTEFKEFDVSIACKAVSILLIK---ISSNLASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGSNETKMEI----------SENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAI--------------------------------------LSSEESTRIGFLNSVKLHLRMLSPILALPRHKTSNQIILVNLGDIEIT-------SDTQSLQVAFSDAIVIS--------KELPIEN-----GGYDIGICTDNNAKFPLVNKIDLMLKFSKLH------------------DPPKIL----------------IECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDEI-----------------PKPFIEFED----ENMQEDQNP--------------DPSENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSY-SNLTSTLIPPPSPFYKYSKR----KKSLSNSVDIQNCAAFELKLPKNTVKSTEP-----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSK----VLPSFNRRYDGEIEE---IINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEF----------------KFENNDRSR------------------------CLLDEMKIDLHEMDVYSAIRI--TDSILKD--GDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYS----SFPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRE-NRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKIC---VIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQT--GYLRHRWSSLNFDNVNTSK----PSQPNLTALQKLSKFVDRISIHHLDDYEFVS-----------------------------VCLIDDCIDADVPLFDIKFNNVTVDIELGNSK-GLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--QER-------------------NVAFCDCR-KRHNNLFVPFLIVNELGCPIEYRKL-------SLDNPPMYNEIWNANKCG-DENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP---LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQN--KP---IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL--------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEG----GILVNKLGMKCYINASCKNAAVFGGFETFKEFYAP--LVYKNWFRPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQS---QLDFNADSSTFSNIEMHFSV-EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVH---VFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLL 11341
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L++L + P VK+G IG++ L+IP+ R S+ W I + + +I G + + ++ E + N M KK LL +E K++++ Q+G++ W S ASL RI+ N+++++++VH+R+ED+ + + F+ G+ ID S ++ E K + KQ+++ S+Y DT+C+++ ++ I+ + + + + I P+ ++KRN + PL PRI +Q+E V + LSQ QY+ ++ E +R +R + K+RP+VS+ + + WW FAI E R AL +RA++ Y + Y L K L + ++ LE E L+ L LR+V + I +K L +N + +IP S W WG +G +SE E+LLP S+ +D+ + + D ++ FTR D +F R EF L +G L ++ + LE + +I+ ES P+ S + + L LF++DL + F ++VS + + S G +F + +E NP+ S +R L +S+ PL I++ P + + E+F + + + AA + K +T I++ +++ + RW + L I AP++ P+ ++ P V++DLGR+ ++N D S KVT N + D +++ TP ++P S + L+ Q ASKE V + +Y++YS+ +++ +VG NW L+ +S P ++ E FN +L L+++ Q P ++ + L + I+ +K+ K CL ++ ++N + + +K Q NL +S + Q+ + V + F ++ + +ES R ++ L + T + T +S+ + L+L D GS D ++ASHK L D P L ES PI+ Y+++Q SS I+ + + SD LD+I N ET+ ELL F++ F + + Q + ++ E TT + ++++ +++LL++ S+ L E R ++ I+ +++N+ +G L+ + S+ + D++ + ++++ + +E E+ S + D +N ++ E + G FV + + Y HS +FL + SE + F + N A T + + E+ FL + L++ + SP++++PR ++++ +LG I+I S Q L+V D + S + P N G + + ++F + + + + H DPP IL IE +F + + + K VYEQVL+++DNL +E + P F + + N+ P PS ++ + L N++++++ L D S+ ++ + Q + EF++ + ++ +++LA+ + + +L+ K LI S + + +E S SCP L +L + + + F Y + S NS +C P + S +P + L + + L+D F T S+ R VD DFN L I+++ WV++LDFFG+GS + VK + VS V PS D EE +NTK+++ + +L+L + + + ++++ +ST Q DG++T+ GSL L DL+P G +Y +R + + L F++ +YG PD +L R+ D+++ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V QR++RI + I AP++++P S+ S +++ +LG+ L N F + N R CLLD M +DL EMD+++A R+ D K DL F +IR L + C L V RNL+ +SH PD I+ LSSV S+ E YR IRG+L NLGE + L+ Y DP ++T L+G + + + + DV L L+ R D +L + +F +SK+ YESFSD SK++ LV + D RY+ A R NVF I+ P K S D + QL + Y S D + F + LN ++ +IFDW+Q ++++ G S +T+ P++ V+ ++ + Q+ + EL +NV + F ++E+ S + T+A+IL +YK R +RP FS S++ + SC + + LSII+P+ I ++ +C V L D + ++ L ++I IRLS++DIQL I +S + PA ++ + G + L +N + + AL +D+ + L++ E VS +CLIDDC+D DVPL ++ F+ + V +G+++ G LS DYYN GW P IEPWP + W T K + ++ L+ +I+S LL+ T + W ++ ++R + D + + FVP+ + N GC + + L +L + + I + + G D++ +S+ F E AR+K+R+ +H H++ +++ GW + +PV+VDK+G+F+R+ D + + V + + R+V I +GS + I +RS L + N L++ +E + V+ + P+ + IPL + +Q RP K + +F+ + + W +V+ PG+ ++ C + F+ S ++N + + I++ N+P A + V PGH + + L N LPCD+ F +K AI + L++P + + + Q+ V L+SFP C LLI + ++ V +RLYD RLL L +++ R G + +++S+ YW+VN+T LP+++ Q +G+ + AAG E+E A S P F++++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G + +T +V RYLLDNRS+ L F Q + P I LP S + FHWPR+D DQLL V+ +D + SGGF+I+ SFHV +R + + LR ITL+GA Y++ FTD + +P P +++NL+ + V F+Q+ + R H+ + P S + YA DEPTLPP + L ++G S D+N LYY+NF+YIA ++F L+ LV DV P ++V L +K RSQLW + L HEGS P K R D+ L+LD+ LA + S ++L + D RR Q W+FT G G+ + +K + A V G ++ P RPGSG + V V+ DGPTRV+++ D ++ S + D + +E+ ++ EG GV L+N +PEEL++ + V+V + ++ + L + I IQVDNQ + +++ S ++ ++ +L+ + P + +F + + +I+EEK+LLK + F EAEL + D +L + + + G ++ Y NLK+ I LSV T+ LP+DLK +K FP L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D+T G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ S+ T +G K EG G F G KG+VG VTKPV G LD A+ +A + R++A S+ R +PRC LL
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALQEL-DLPFEVKAGFIGKIRLQIPFYRPHSEPWVISMSQLNLIIG-LAQLQEYDAEEERRNEMERKKHLLTILEEKFKRECEQRGES------YWYSVTASLVTRIVENIELKIQDVHLRFEDDISNSEKPFSFGVCIDNVSAQNPSKESMVKL-------LRQKQLEIQNFSVYWDTECEMLGKLPASQIQDAMMQCMQSR-EHQYIFEPVCASVLLKRNTSKEPLRSRQTPRIEGQVQLEPVALRLSQVQYQQMMSFLKELDRRERGMLYRKWRPKVSISGNCRMWWSFAIQANLNDIREQRRRGSWSFAL-QRAQDAQTYTRIYFKRL--KGVPLSPQEKSQLERVEEEQTLEELHGLRDVVYDWI-QKQEQLPENTREPSSELQPVPTSTSIPKSGSSRMIQYLQSWFPGWGGWYG----------------DSEDPDRPEELLPSPSSWDILAETEDLFDPME--DSHTLSTFTRRDHMFARLEFFLHKGEVTLRHQGKTAHTLHESALIQLEFSGVKIIL--ESLPRSDSSLLSVKLGGLFLKDLT--TQGTIFPFLVSPKGDDVDCLFNPSLSQFSSTSGAGAACTSPVFEMIYERNPVRS----KFERRLEVSTSPLNIIYNPQAMKKVTEFFYKGRIHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENGKRWTMKLDICAPQVFFPD-DFQSENPMLVVVDLGRIFLTNSEDGSTVNS-KVT----QNDKEDFSDDEYQTPPATPPESPVPELNTPLKAQPKSPELPSLASKEGAQVYSKLLYEKYSLSFKDLQIMVGHCNDNWKHLQ-----ESSVGPTHVV-EKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHINLEKMIALKSCLSRLSGPALSGGEIITMSPEPLTVHNDKISQKVDSSWKLQGSAKNLTQSVMLLEQHTREVLVESRLLLAEFNINYMQLGVESDGRYISVLKVFGTNAHFVKRPYDTEVSLTVHGLLLVDTLQTYGSDYDLLVASHKHLSFDIPTGSLRESQPSSPILCDGKSPEYQLNQTEFSSSCPIRGMSPVMPILKDQEALIKLEYQFVSSDSPCMNLETSLQVTSMQVHNLDIILNPETMLELLKFLQKSFPKEESSLTASAQQGAAQPYSEEEEKLYQTTYCQTKELTVEIHRLNLLLLRSITASTALVPEKRVLKIATASITGTKVNV---SHGCCLDIRGSLGSMQLVDLTPEGGQSQFVVSIGS-VDENTSELDGLAYFSDKRSTSADALNFELM-EKSQGECFVKLDMASLHYSHSAKFLWELSLSASELEDSFHSMLKNAATRVSTVLATKTAEYSNMVSLFETPLRRVRTASQSFADSFEDEQVNVEEPETHSDSFLVKLTLNINIESPVVSIPRKPGHPELLVGHLGSIKIQNFMNGHGSKEQRLEVLVKDIRLYSLNMHNIVLRRAPKANPPDRLSGPHGEVTRPDKSQFTRHDFFESLSRGKAFHILKDTTVQFNVEKHPIKEDPPLILTTPFHNQICRSSEQLRIEGKFVNPVQVCLCKTVYEQVLQTLDNLFLSEEQQATSSHPPTPPPPTPSSARPHTFPDLQGGLFARNLPTVNFPHLSLSSPLSLQPHTSPSFTQLRVTL--NVAELQVMLSADLSQGSQG-LVSVCFQDLEGEFIKTHPHLLEVQLALRSLLMEDLLEHNPDSKYKYLILSHGAPKPPTFSPKEYLSQSCPSTSNALYPDMPRSLPAHMEEAQNVFQLYQRHPSTPTSSSRNSKRDPDCPITPPPSPTHYRASPQPLSEFDDSLVHMNLLLVDPNHPEFKTRYWSVGRSVDIDFNCLDVLITLQTWVVILDFFGIGSTASN-HAVK--VPVSPWPGPVHPS--SEPDASREEPVQSVNTKVDLKVHSLNLVLNKKLNELARASVSKLST-----QLEVLDGDLTIHGSLGSLSLSDLTPHGDLYRERF--VTQGGKALVFNILRYGCPDVNLKRDCDIKVCLQMAS-VQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAVEGQAVRDLPQRASRILLDIEAGAPVIVIPESSRSTKVIVANLGQLRLQNCFLSAGAPSTFHKEGVSSDEQENPRCSSSDTPFNLGSAPEPLGLLSTEGHVCLLDVMALDLQEMDIFAAERLFSPDGTGKPEPSDLIFPSFSIRRTGDNLLRDCCRLKFKVERNLDKELSHAVPDLSIHVSLSSVHCSLDREHYRLIRGLLEKNLGEPIEEFLRPYNL----LDPSIYTVLSGDVFINLSFLVDMTDVSLELLDR--PADTEDRHSLAKFDFMKSKVLYESFSDGSKSVNLVSHSLLAHDTRYTRPRKGAEATPR-NVFDCILQPSKAG--SNDA--SLQLELHYRSTRDASCFTMVLNNLRVFVIFDWLQRLQDFL-QVHVGKASDSTETHLLRSSSARSSGAVIPKTVKSG---VVTKRSTVPVSQD--RCLELKVNVTGTEFVVVEDMSCVDTNAIILKGTTVLTYKPRLLDRP--FSGSLAGVEVFSCRLGREQETALSIIDPINIQVE----LCGNPVYRSTSGLLDAFNVEDIPPL-LEIQFPVLDIRLSYNDIQLFLTIAKSIPSAKALPAPVSVEVTPGTSKQETHHLKETQLNHLQDLGFKKEDCRRALSLCKGHLDQAATWLLENAENVSRCSSTRVDSSTSSHTAPLSGVELRADSICICLIDDCLDCDVPLAELTFSRLNVLQRIGSTREGNASCTLSGDYYNRELSGWEPFIEPWPCCLSWQQQTGGHLQPPRFKMEVQAKQRLDINITSALLEQYTTTKRSWMADYWVEDRQELKTSPTLPWIGSSVDPPSFGQTDMKLSKRRQPFVPYTLRNHTGCTMRFATLTTTPTRVALSHSNSADSISDIHSSGNDDSHKVSLWREVLPGEEFSFEFEAREKLRHRH--THELKLHQLLVQVCGWEQVKPVSVDKVGVFFRYATPDRNYSSNTVGSPVSKTNIIHPHVYFSALPPVRVVFAITMEGSARKVITVRSALMVKNKLDVLMEVRLDSPSAPDKPVVLPPIPPSQALAIPLHLTSWRLQARP------KDMG------MFFCKVPIH-----WTSVDRPGEISSSKREC--QLADFDDSLKRNFRFCV-VIKKENYPDQQPAKRQVSGSVHHIYQQPGHTVYLMPTMVLVNVLPCDLNFYIKGT-------AI--KGLLKPGKEAVLHSADTSQNMELGVLLESFPVCKELLIPPGTQ-NYVVRMRLYDTSKRLLCLTIRIVLRAQGALKILVSAPYWLVNKTGLPVIFRQDNGKAD--AAGQFEEHELARSLSPLLFSYTDKEQPSMCTMRVGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQQGNRPGLIYNIGISVRKGKGNYRDTHIVTFAPRYLLDNRSTHKLAFAQREFARGKGTFNPEGYICTLPGSSVVFHWPRNDYDQLLCVRLMDMTN---CTWSGGFEINKPKSFHVNMRDMRGS-CFYLRTEITLKGATYQISFTDTEHMPPPLRVDNLSEVPVQFWQH-GVADLRLHTEVKPLSVLDYACDEPTLPPYLTLTVKGAGSSEVTADMNFFREYNKLYYENFIYIAATHTFSQKLERKRRLISHTELVLDVDPKTQRVILQKKEPGKRSQLWRMTGTGMLCHEGSSPPQS-KPSQPRPLDSSLVLDIAGLA---AVSDNNYEPLMLRRPDSRRSTTQTWYFTSGMLTCGLPRLVVQVKGGVTGLSDGAEVVLGPDSGLLEPGPEQQFINQKMRPGSGVLSVQVLPDGPTRVLQISDFNQRRVVRSSTSPEQDKCREDVQKKELEVLVNLDEGLGVSLVNKVPEELIFTTLSGVNVHFTHTASSEVLELNIQNIQVDNQLLGTTQPVILWMTPSSGDSGTNNSSPALQVN-SVKVPSSLMLTTLFKHLMVTARHFTVIIEEKLLLKLLSFFGYGEAEAELEKLD-ENLHERPNEEAGPPQRYYFENLKISFPQIKLSVFTSHKLPADLKALKGTLGFP-LVRFEDAIINMYPFTRVHPYETQEIIINDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQNEREYIRYHGATSGEH---LVAGIHGLAHGIIGGMTSIITSTVEGVKTEGGVGGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSNHRFSVQRVRKPRCCRGPQGLL 4150
BLAST of Vacuolar protein sorting 13 homolog D vs. TrEMBL
Match: A0A484D455 (Uncharacterized protein OS=Perca flavescens OX=8167 GN=EPR50_G00071070 PE=4 SV=1) HSP 1 Score: 1109.36 bits (2868), Expect = 0.000e+0 Identity = 1142/4385 (26.04%), Postives = 1950/4385 (44.47%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMMKKL----LLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQN-------------AVNIPAPQQSG-------W---WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLEGIKLKLL-------KDTEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKV-----PGGE--------------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGS-----------------LENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTN-----------FMNNYRNADSSSVKYKK-----------QTRMDNLNKSP-EINQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSD---------QPLSESIGD---------------------------------PIIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSND------SLQIFDQKP------ETPNTTEFKEFDVSIACKAVSILLIKISSN---LASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGSN-----------ETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAI---------------------------------------LSSEESTRIGFLNSVKLHLRMLSPILALPRHKTSNQIILVNLGDIEIT-------SDTQSLQVAFSDAIV-----------------------------ISKELP---------IENGGYDIGICTDNNAKFPLVN---KIDLMLKFSKLHDPPK----ILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDEI-----------------------PK--------PFIEFEDENMQED-----QNP--DPSENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNSVDIQ-----NCAAFELKLPKNTVKSTEP-----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEE-----IINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVN---------EFKFENNDRSR--------------------------------------------------CLLDEMKIDLHEMDVYSAIRI-TDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENT-DHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPA------NKRLNVFPIIISPDK-NTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQT--------------IPPANLAYQT------GYLRH--------------------------RWSSLNFDNV------NTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQER---------------------NVAFCDCR-KRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLK---PNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP---------LAPKMV---PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEG----GILVNKLGMKCYINASCKNAAVFGGFETFKEFYAP--LVYKNWFRPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQS---QLDFNADSSTFSNIEMHFSV-----EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYA---CVMYEENKSESPSSSQNAFSLKFQRELGHPYDV---HVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAEL--LEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRY-EPRCLSSSWKLLPYQTS 11356
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L + F+ P VK+G IG++TL+IP+ R S W I + + +I GP +L + + + E +K LL ++E K++ + Q+G++ W S AS+ RI+ N+++++++VH+R+ED+ + P + + G+ I+ FS ++ E T + + KQ+++ S+Y DT+C+++ ++ I+ + + + + I P+ +++RN + PL H PRI +Q+E + + LSQ QY+ +A E +R++R+ + K+RP+V + + ++WW+FAI E R G AL +RA++ Y Y L S+ E +E + L+ L LRE+ + RK ++ +N +IP SG W WG +G PE +LLP S+ +D+ + L D +N FTR D +F R EF LE + L K E ++++ + V ES P+ S + + L LF+RDL + F +VS + V P G+ +F + +E NP+ C +R L +++ PL I++ P I + E+F + + + AA + K +T I++ +++ + RW + L I AP++I P+ ++ +P V++DLGR+ ++N D S K T PE D+ +++ TP ++P S L +L QA + +Y++YS+ +++ +VG NW L ESE+ ++E FN +L L+++ Q P ++ + L + I+ +K+ + CL +++ N R+ D +++Y K Q NL +S + Q+ + V + F ++ + +ES R ++ L + T + +S+ + L+L D GS D ++ASHK L D QP S + D +I+ Y+ +S S ++ + + ++LD+I N ET+ ELL F++ F + S+Q +P T T + ++++ +++LL++ S L +E + ++ I+ +++N+ M G+ L+ + S+ + D++ S + ++++ + + +T SE +N ++ E + G + + + Y HS +FL + +E + F + N A T + + EST FL + L++ + SP++++PR ++++ +LG I I S+ + LQV D + I++E P + G I D +F L + D F DP K + IE +F + + + K VYEQVL+++DNL +E + P+ P+I F ++ P D S ++ I L ++++++++L D S+ ++ L Q + +F + + ++ ++LA+ + + +L+ + K L+ S + + +E S SCP L I ++ +R S ++ + +C + P + S +P + L I + L+D+ F T + R +D DFN L I+++ WV++LDFF +GS + VK L + P N Y+ +I E +NTK+++ + SL+L + +N A VS + L+ DGN+ L GSL L DL+P G +Y +R + L F++ KYG PDP L RE D+++ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V QR++R+ + I AP++L+P S+ S +++ +LG+ + N F + +R R CLLD + +DL EMD+++A R+ + + DL F ++R L + C L L V RNL+ +SH PD I+G LSSV+ S+ +E Y+ IRG+L +NLGE D L+ Y Q DP +T L+G Y + + + DV L L+ + +L R +F +SKL +ESFS+ SK++ LV + D R + A R NVF I+ P K T R+ + QL + Y S D + F V LN ++ +IFDW+Q+ ++++ V A + R+ H + S P S + + P V+ ++ + Q+ + EL +NV + F ++E+SS L T+A+IL +YK R +R PFS S++ + SC + S+ LSII+PV I + ++C G P+ L D + ++ L ++I IRLS++DIQL I +S + + P + Q G L H W N +N+ T S + L + + + I +DD CL D D+PL ++ F+ + V +G++K +F LS DYYN GW P IEPWP + W K + ++ L+ +I+S LL+ T W ++ + + + D + + FVP+ + N GC + + L+ + ++ E D+ S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+G+F+R+ D ++ + V + I R+V I +GS + I +RS L + N L++P++ + + S+ VVL P + +PL + +Q RP K + LF+ + + W +V PG+ ++ C S + F+ S++ N + + I++ N+P + K + PGH I + L N LPCD+ + +K + S+ +P + + Q+ V L++FP C LLI + ++ V +RLYD LL L +++ R G + ++IS+ YW++N+T LPL++ Q +G+ + AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDN S+ L F Q + I LP S + FHWPR+D DQLL V+ +D+ P SGGF+++ SFHV +R N LR ITL+GA Y++ F+D D LP P++++N++ + + F+Q+ + R H+ + S + YA DEPTLPP + L ++G S D+N LYY+NF+YIA ++F +++N LV DV ++V L +K RSQ+W + L HEGS P K R D+ L+LD+ LA + S ++L + D RR Q W+F+ G G+ + +K C A V G ++ P RPGSG + V V+ DGPTRV+++ D ++ S + D D E V EG GV L+N +PEEL++ + ++V + R + L + I IQ+DNQ + CV + S S+S + +L+ + P + H+F + + +I+EEK+LLK + F AEL LEE+ + + D G ++ Y NLK+ + LSV T+ LP DLK +K FP L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D++ G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ S+ T +G K EG SG F G KG+VG VTKPV G LD A+ +A + RE+A S +R +PRC LLP ++
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRE-FDLPFEVKAGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPA---QLLDYDGEKEREEEKERKTRLLKALEDKFKSECEQRGES------YWSSVTASVVTRIVENIELKIQDVHLRFEDDVSNPEKPSSFGVCINNFSAQNPSKE-------TVQKLLRQKQLEIEDFSVYWDTECEMLGKLPTNQIQDAMTQCMQSR-EHQYIFEPVCASVLLRRNTSKEPLRSRHTPRIEGQVQLEPMSLRLSQVQYQQIMAFLKELDRWEREMLYQKWRPKVPISGNCRQWWMFAIQANLSEIREQRRRGSWGFAL-QRAQDAQTYTSLYFRRLKGVTLSSQEEIELERVEEEQTLEELQSLREIVHDWF-RKQEEIAENVREPSSDPQPCLPTTSIPKSGSSGMIQYLQSWFPGWGGWYGDSQDPDRPE----------------ELLPSPSSWDILAETEDLFDPLE--DSHTLNTFTRRDHLFARLEFLLEKGAVTLFHQDRRADKLHESGLIQLEFSGVKVILESLPRSESSMLSVKLGGLFLRDLT--TQGTIFPVLVSPKTDKVVVVMNQPSGQSSSTSGCNAECSSPVFEMIYERNPV--RC--KFERRLEVNTSPLNIIYNPQAIKKVTEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKRWTMKLDICAPQVIFPD-DFQSEDPMLVVVDLGRILLTNSQDD-TKASSKATQPE----REDMSDDEYQTPLATPPESPPPELDIPFTEQLKSPELPSLRSPESSQAYSK-----KLYEKYSLSFKDLQIMVGRCKDNWKHLQESEVGPTH-------VVEKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHINLEKMTALRSCLARLSSPSVSEGDKSQERGPNIRSPDPLTLRYDKIFQREDSSWKLQGSAKNLTQSVMTLEQHTREVLVESRLLLAEFNINYMQLGVESDGRYISVLKVFGTNAYFVKRPYDAEVSLTVHGLLLVDTLQTYGSDFDLLVASHKNLSFDIPTGSLRESQPSSPVLSDGRSPGHQGHHTESSSGMPVDRISPFSSFLKDQEALIKLEYQFVSSDCPSMNLDSSLQVTSMQVNNLDIILNPETVVELLKFLQKSFPKEESTWTSSVQQHTTQPCSEEEEGTYQATYDQNKELTVEIHRLNLLLLRTVSTGTVLGAEKKGLKIATASITGTKVNVSM---GSRLDINGSLGSMQLVDLTQEGSRSQFVVSIGSADSCSTPDGLAFLTDTGGSPSE---ALNFRLM-EKSQGECSLQLQMASLHYNHSAKFLKELSLSANEVEDNFRSMLKNAATKVSTVLATKTAEYSGMVSLFETPSRRTRTQSQSCSYPFEDEEDLPMEEPESTLDTFLVKLTLNISIESPVVSIPRKPGHPELLVGHLGSITIQNFVTGQDSEGERLQVLVKDIRLYSVNMSHLVLKMAPKGEKAGSLSPSHHKSINQEEPQFTRHDFFESLSRGKAFHILKDTTIQFTLEKVPVEEDTQFTFLTTEDPCKSKGLLKIEGKFVNPVQVFLCKPVYEQVLQTLDNLSLIEEQRVTPSQPPTPPPPTPSSTRPHRFPDPQGGLFARDPPYISFTHSSLSSPLPLNMHKPALDTSFTQLKITL--HVAELQVQLSADLTQGSQG-LVSLRFQDLEGDFTKDHPHLLSVQLALRSLLMEDLLEPNPESKYKHLMVSRGAPKPSTFSPKEYLSQSCPSSSNALYPDMPRSLPAHIEEAQNVFQLYQRHPSTPSASSRKSKRDPDCPSTPPPSPTHHTPSPQPSPDFDDSLVHINVLLVDQNHPEFKTRYGRVGRSIDVDFNCLDILITLQTWVVILDFFSIGSTANN-HAVKGLPMSPQPEPG-NPLYEPDISEEETTEAVNTKVDLKVHSLSLVLNKK---LNELARASVSKLSAHLEML--DGNLALQGSLGSLSLSDLTPHGDLYRERF--TTRGGEALIFNIQKYGQPDPHLERECDIKVSLQMAS-VQYVHTQRFQAEVVAFIQHFTQLQDVLGRQRAAMEGQAVRDHPQRASRVLLDIEAGAPVILIPESSRSPRVIVANLGQLRVQNCFLPAGAHGTFSLRDKERVRSATGRSSEQKPKSPPSSSSSSTGFSLGRPQAPDTARASEVDEPHSLEEHVCLLDCIALDLQEMDIFAAERLPCGNPPESSDLIFPSYSVRRTGDNLLKDCCRLKLKVERNLDKELSHAVPDMSIHGSLSSVLCSLDLEHYQLIRGLLENNLGEPVEDFLRPYNLQ----DPSTYTVLSGDVYTNLSFLVDMMDVSLELLDSPTPTEQ--KHSLARFDFMKSKLLFESFSNGSKSVNLVSHSLLAYDTRCTGPNASSAGVDGARRNVFDCILQPSKTGTNRA-----SLQLELHYRSTRDSSCFTVVLNNLRVFLIFDWLQLVQDFLRLPGEKAVGTA-EPCQRWPSHTS--TDSGPSSSTGAVMPKTVKSGVVTKRSTVPVTQD--RCLELKINVTGTEFVVVEDSSCLDTNAIILKGTTVLTYKPRLLDR--PFSGSLAGIEVFSCRLGSELETALSIIDPVNIQV----ELC---GSPTYQSSSGLLDAFNVEDIPPL-LEIQFPTLDIRLSYNDIQLFLAIAKSLPTASSATPSDCDASSEPVVAPKDSFRQKTEALIEGQLTHLQDLGFRKEDCKRALIHCKGQLDQAATWLLENAENIVGHAKARTDSGSSSHSAPLSGVELKAEGVCICFIDD------CL-----DCDIPLAELTFSRLYVLQRIGSTKEGNASFTLSGDYYNRELSGWEPFIEPWPCFLSWQQQAAGRLHPPRFKMGIRAKQRLDVNITSVLLEQYNTTKSSWIADYCNKEGQTPLSSPPLPWMGSSVDPPSFSQTDVKLSKRRQPFVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISEVHGPGADDTHNVSQWREVLPGEEIPFEFEAREKLRHRH--THELKLHQLLVRVCGWEQVKPVSVDKVGVFFRYAAPDRNNSSNTVGSPISRTNIIHPHVYFSALPPVRVVFSITMEGSARKVITVRSALMVKNRLDVPMD-VRLDSPSAPDKPVVLPPVLPGQALAVPLHLTSWRLQARP------KGLG------LFFCKVPI-----HWTSVERPGEISSSKRECQS--ADFDDSRKHNFRFCV-VIKKENYPEQQPAKSVSGSAKQIYRQPGHSIYLLPTMVLANLLPCDLNYYIKGTSIKGSL---------QPGKEAELHAADTSQNMELGVLLENFPVCKELLIPPGTQ-NYVVRMRLYDTNKHLLCLTIRIILRAQGALKILISAPYWLINKTGLPLIFRQDNGKAD--AAGQFEEHELARSLSPLLFCYTDKEQPAMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGISVRKGKGRYRDTHIVTFAPRYLLDNHSTHKLAFAQREFARGKGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDT---PNCTWSGGFEVNKLKSFHVNMRDTLGN-CFFLRTEITLKGATYQISFSDTDQLPPPFRIDNISEVPIQFWQH-GVADLRLHTEVKAGSVLDYACDEPTLPPYLTLTVKGAGSSEVTSDMNFFREYNKLYYENFIYIAATHTFSPNIENRPAGKKRLVSCAELVLDVDTKTQRVILKKKEPGKRSQMWRMTGAGMLCHEGSSPPQS-KPAQPRPLDSSLVLDIAGLA---AVSDNSFEPLMLRRPDSRRSTTQTWYFSSGMLTCGLPRLVVQVKGGGAGLCDGAQVVLGPDSGLMEPGPGQQFINQKMRPGSGVLSVQVLPDGPTRVLQISDFNQRRMIRSSASTEQDRAKDPVKKGEPEQELEVLVNLEEGVGVSLVNKVPEELVFTTLSGINVHFTRIAANQVLELSIQNIQMDNQLLGTTQPVMLCVTPSSSDSSDSSASDSGPALQLN-SVKVPSSLVLTHLFKHLMVTARRFTVIIEEKLLLKLLSFFGYGQTGAELEKLEEN---IHEKPSEDAGSPKRYYFENLKISIPQVKLSVFTSHKLPPDLKALKGTLGFP-LVRFEDAVINMYPFTRVHPYETQEIIINDILKHFREELISQAAQILGSVDFLGNPMGLLNDVSEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHGATSGEH---LVAGIHGLAHGIIGGMTSIITSTVEGVKTEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVREMASLSNHRLSVQRVRKPRCCKGPQGLLPRHSA 4205
BLAST of Vacuolar protein sorting 13 homolog D vs. TrEMBL
Match: W5UKH0 (Vacuolar protein sorting-associated protein 13D OS=Ictalurus punctatus OX=7998 GN=VPS13D PE=2 SV=1) HSP 1 Score: 1107.82 bits (2864), Expect = 0.000e+0 Identity = 1132/4369 (25.91%), Postives = 1924/4369 (44.04%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGA-------ALRERARNLVKYVQSYEDYL-LNKDSNLEAEFRNNLESDLELDCLLQLREVA------MEHIGRKSRDLLQNAVN------IPAPQQSGWWGYL--WGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLEGIKLKLLKD-------TEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKV----PGGE-------------------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRS---TENIKAAAEKHIRNA--------------KARTSESIKKIL--EKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDF-----------VTPCSSPFGSLENLSMATDCQASKETKPVTNI-YDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSS------------------SVKYKKQTRMDNLNKSP-EINQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSD--------------------QPLSESIGDP----------------IIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDS---LQIFDQKPETPNTTEFKE---------FDVSIACKAVSILLIKISSN---LASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGSNETK----------MEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEES------------TRIG------------------------------FLNSVKLHLRMLSPILALPRHKTSNQIILVNLGDIEIT-------SDTQSLQVAFSDAIVIS---KELPIENG-------------------------------GYDIGICTDNNAKFPLVNKI----DLMLKFSKL----HDPPKILIECEXXXXXXXXXXKAVYEQVLKSMDNL-VYDDEDEIPKPFIEFEDENMQEDQN---PDPSEN-------------------EIPIVLQSN-----------------ISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLS----NSVDIQNCAAFELKLPKNTVKSTEP-----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYD----------------PKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNE---------FKFENNDRSR-----------------------------------------------------CLLDEMKIDLHEMDVYSA----IRITDSILK--DGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSF--PANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRE-NRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIR------------SFQNQ----TIPPANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKL--------------SKFVDRISIHHLDDYEFVS----------------------------VCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICW-------FWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERN---------VAFCDCR-------------KRHNNLFVPFLIVNELGCPIEYRKLS-------LDNPPMYNEIWNANKCGDEN-----KWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKP---NTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP--LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPLVYK--------NWFRPGSGEVLVSVVTDGPTRVM-------RLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVE-GFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYA---CVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGRK-IYIGNLKLLTGVISLSVITAESLPSDLKQMKK-LFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRY-EPRCLSSSWKLLP 11344
M+ L+ ++L+ +G+YV N +T + L KG + LENLPL+ L + F+ P VK+G IG++ L+IP+ R S W I + + +I GP + E+E + E KK LL ++E +W+ + +Q+G++ W S AS+ RI+ N++++++ VH+R+ED+ + P + FA G+ I S +S E T+ + KQ+++ S+Y DT+C ++ ++ + I+ + + + I P+ +++RN + PL H PRI +Q+E + + LSQ QY+ +A E +R +R+ K+RP+VSV E+ ++WW+FAI+ + I + AL +RAR+ Y + Y L L S E +E + L+ L LRE+ E I SR+ + + IP SG YL W + + + ++L+P S D+ + L D + +N FTR D +F R +FSLE + L ++ E ++++ + +G ES P+ S + + L LF+RDL + F ++VSS+ + P G+ +F + +E NP+ S +R L +++ PL I++ P I + ++F + T +E +R A KA ++I ++L E + RW + L I AP++I P+ ++ +P V++DLGR+ ++N + + K + + + + E++ + + + E S + ++ + + I Y++YS+ ++ +VG NW L+ T ++E FN +L L+++ + Q P ++ + L I I+ +KL + CL + + + N + ++K Q NL KS + Q+ + V + F ++ + +ES + ++ L + T + +S+ + L+L D GS D ++ASHK L D P + +G P +I+ Y+ +S S +++ + + ++LD+I N ET+ +LL F++ F + Q P PN + +E ++++ +++LL++ S+ L SE R ++ I+ +++N+ M G+ L+ + I + D++ + ++++ + T M ISE +N + E + G + + + Y HS +FL + +E + F R AA + +L+++ + +R G FL + L++ + SP++ +PR ++++ +LG I I S+ + LQV D + S L + G G I D +F L K+ D F H + +E +F +++ + K VYEQ+L+++DNL + +DE I + + PDP P+ LQ++ +S+++++L D S+D ++ L Q + +F + + + I+LA+ + + +LD + K L+ S + N +E S SCP + + +++ +R S S S C + P S P + L I + L+D++ F T SI R VD DFN L I+++ WV++LDFFG+GS + P L D++ VL N E EE +NTKL++ + SL+L +T N A VS + L+ +G++ GSL L DL+P G +Y +R + L F++ KYG PDP L+RE DM++ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V + QR++R+ + I AP+LL+P S+ S +++ +LG+ + N F ++ DR + CLLD + +DL EMD+++A + DS +K D DL F ++ L E+C+L L V RNL+ +SH PD I+G LSSV S+++E+Y+ IRG+L HNLGE + L+ Y Q DP +T L+G Y + + + +V L L+ + + + L R +F +SKL +ESFSD SK++ LV + D RYS ++ NVF I+ P R+ + QL + Y S D + F V LN ++ +IFDW+Q+ ++++ T+ + R+ + + V+ ++ + Q+ + E+ LN + F ++E+SS T+A+IL +YK R +RP FS S++ + SC + S+ LSII+PV + ++ +C G P+ L D + ++ L ++I A IRLS++D+QL I + +F +Q T P RHR +L + LT LQ L + +D+ + L++ E +S +C IDDC+D DVPL ++ F+ ++V +G+++ +F LS DYYN GW P IEPWP + W + K + ++ L+ +I+S LL T W ++ + + V++ + F+P+ + N GC + + L+ L + + I + + G ++ +W V F+ AR+K+R+ +H H++ +++ GW + +PV+VDK+G+F+R+ D ++ + V + I R+V I +GS + I ++S L L N L +P+E I + S+ VVL P + IPL + +Q RP LF+ + + W +V PG+ ++ C S+ F+ Q K I++ N+P KMV PGH I + L N LPCD+ + +K + + ++P + + Q+ V L++FP C LLI + ++ V +RLYD RLL L +++ R G + ++IS+ YW++N+T LPL++ Q ++T AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDN+SS L F Q + I LP S + FHWPR+D DQLL V+ +D P SGGF+++ SFHV +R L I L+GA Y + F+D D LPSP++++N++ + + F+Q R H+ + + YA DEP LPP + L ++G S D+N LYY+NF+YIA +F D LV DV ++V L +K RSQLW S L HEGS P K R D +++LD+ LA +S + + +L + DPRR Q W F G + + +N + ++ G E + L+ K RPGSG + V V+ DGPTRV+ R I++ S + + T +E+ ++E G G+ L+N +PEEL++A + +++ + R + L + + IQVDNQ + C+ S S A + + +F + + +I+EEK+LLK + F E E+ + D + + S RK Y NLK+ + LSV T+ LP DLK +K+ L L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D+T GM L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ SV T +G K EG +G F G KG+VG VTKPV G LD A+ +A + R++A S +R +PRC LLP
Sbjct: 1 MLEGLVAWVLNTYLGRYVSNLNTDQLSVALLKGAVELENLPLRKDALRE-FDLPFEVKAGFIGKIVLQIPFYRPHSDPWVISMSQLNLIIGPAHPQEYDEEQEREAEREQKKQLLKALEDRWKSESKQKGES------YWYSATASVVTRIVENIELKIQGVHLRFEDDFSNPEKPFAFGVCIKNVSALNSSKE-------PTQKLMRQKQLEIEEFSVYWDTQCTMLGDLPLAEIQETMTVCMQSR-EHQYIFEPVCASVLLRRNASKEPLRSRHTPRIEGQVQLEPLSLHLSQVQYQQIMAFLKELDRREREILFRKWRPKVSVSENCRQWWMFAISANLEEIREKQRRSNWDFAL-QRARDAQLYTKLYGQRLKLVTLSQQEESELERIEDEQTLEELQILREIVHESFRKQEEIAECSRETISDPTASSPSGAIPRSGSSGMIQYLQSWFPGWGGWY----GGGERGPDGQPVTDELMPGSSQWDILAETDDLFDPLE--DSQTLNTFTRRDHLFARLDFSLEKATVTLFQEDKLNRLSNESGVIQLEFSGVKIGVESLPRSESSLLSVKLGGLFLRDLT--TQGSIFPFLVSSKNKIALTFSQPFGQSSSTDKSSTGPDMESSASPVFEMIYERNPITS----RFERRLEVNTSPLNIIYNPQAIKKVADFFYKGRVHTSGFGYQSELELRVADAVRKQYNKLKIQTKAEIRQTIDQLLVGEFIENSKRWTMKLDICAPQVIFPD-DFQSKDPMLVVVDLGRILLTNSQEDL-----KTKSKDSQSKGDEFSDEEYQTPPATPPGSPPSEPETEYKGQEKSSDPSSLKSMEGAQSFGKILYEKYSLSFNDLQIMVGRHKDNWIHLQENEVGPTH------VVEKFNVLLQLEQRLRYTSNPQLPGAVLSGTLPDLKIHINLEKLTALRSCLARLGSPSADEANTGETIKSPEPLPLRHEKIFEREDSQWKLQESDKNLTKSVMTLEQHTREVLVESRLLLAEFNINYMQLGVESEGQYISVLKVFGTNAHFVKRPYDAEVSLTVHGLLLVDTLQTYGSDFDLLVASHKHLSFDVPTGSIRESQPTSPVSQDGKSPETSDLGVPFEKISSFSSFLKDQEALIKLEYQFVSSDCPSMNLESSLQVTSMQVNNLDIILNPETIVKLLRFLQQSFPKEESTWTPSTQQMPSLPNDQDQEENYQSTYDQNKELTVEIHRLNLLLLRTVSSGTALGSEKRGMKIATASINGTKVNVSM---GSRLDVNGSLGCIQLVDLTQEGGRGQFVVSIGNVEDSTSVDAVFPSSALMPISE---ALNFHFL-ERSQGECSLELHMASLHYNHSAKFLKELSLSANEVEDNF-RSMLKTAATKVSTVLATKTAEYSYMVSLFDTPSRRGRSHSQCNWPLESFEDHNDPADLETEPSVDSFLVKLILNVSIESPVVFIPRKPGHPELLVGHLGSIAIQNFIAGQDSEREKLQVKVKDIRLYSVNTSHLFLRKGPNSLQSPSHKTVFNLDEPQFTRHDFFEYLSRGKAFHILKDTTIQFTL-EKVPAVEDTQFSFQSFDGSYHSTGLLRVEGKFVNAVQVFLCKPVYEQLLQTLDNLSLIEDEKPISRQPPTPPPPTPSSTKPYRFPDPQGGLFPRDPSLDVSYSSISSFLSTPLPLQNHSSSVELRSFTQVRATFKVSELQVQLSADLSQGSQD-LVSLRFQDLEGDFTKDHPHTLSIQLALHSLLMEDLLDQNPESKHKHLMVSRGAPKPSTFNPKEYLSQSCPTASSAVFPEMPRSLPAQMEEAQNVFQFYQRHPSTSASSRKSKKDSECPSTPPPSPSRHTPSPHPLPDLDDSLVHINVLLVDRRHPEFQTRYGSIGRSVDVDFNCLDVLITLQTWVVILDFFGIGSTANNHAMKVPSTSPQSMSGQPHADTALADLTDVLSEINEE---ESEEKVNTKLDLKVHSLSLVLNKKT---NELAKASVSKLFTHLEMI--EGDLAFQGSLGSLSLSDLTPHGDLYRERF--TTRGGEALIFNILKYGEPDPFLVRECDMKVSLQMA-SVQYVHTQRFQTEVVAFIQHFTQLQDVLGRQRAAVEGQAVREHPQRASRVLLDIEAGAPVLLIPESSHSKRLIVANLGQLKVRNSFLLAGTHGTFSLKDKDRVKNAQPTSGKVCEEVSADRVKASSTTVSGFTLGKPQPVMGHSSDADTIISPEDHVCLLDCIAVDLQEMDLFAAEWMPCELWDSGVKRIDTDLVFPSYSVHRTGGSLLKERCHLKLKVERNLDRELSHAVPDMSIHGSLSSVHCSLALERYQLIRGLLEHNLGEPVEEFLRPYNLQ----DPSTYTVLSGDVYTGLSFLVDMKNVSLELLDGSKSSGHKQS--LARFDFMKSKLLFESFSDGSKSVNLVSHSLLAYDTRYSGSNKATERKHNVFDCILQPS----RTGTNRASLQLELHYRSTRDSSCFTVVLNNLRVFLIFDWLQLVRDFLHTS---VEKGEAPTRQRWPSSSSDPGTTGAVMPKTVKSGVVTKRSTVPVTQDRY--LEVKLNATGTEFVVVEDSSCSDTNAIILKGTTVLTYKPRLLDRP--FSGSLAGIEVYSCRLGSEQETALSIIDPVNVQVE----LC---GSPTYQSSSGLLDAFNIEDFPPL-LEIQFPALDIRLSYNDVQLFLAIAKSIPTGSPSVTDPAFASQAVLSTASPETSTKPKDSFRHRTEAL----------LESQLTRLQDLGFRREDCRRALIHCTGQLDQAATWLLENAESISGRNRADSDSGSHSSPPLSGVEVKAESVCICFIDDCLDCDVPLAELTFSRLSVLQRIGSTQEGKASFTLSGDYYNRELSGWEPFIEPWPCVLNWQQQAAGRLHPSRLKLGVQAKQRLDMNITSVLLDQYSTTKTSWLADYCKEDEQSPQSSASVSWMGSSVDPPTFGQTEVKLSKRRQPFIPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISDVHGPGTDDTHNISQWTEVLPGQEIPFEFE----AREKLRHRH--THELKLHQLLVRVGGWEQVKPVSVDKVGVFFRYAAPDRNNPSNTVGSPISRTNIIHPHVYFSALPPVRVVFSITMEGSARKVITVQSALMLKNRLEVPME-IRLDSPSAPDKPVVLPPILPGEALAIPLHLTSWRLQARPKGLG------------LFFCKVPIH-----WTSVERPGEVSSSKRECQSA--DFD-DQHKRSFRFCVVIKKENYPDQQPAKMVTGSAKQIYRQPGHTIYLLPTLVLTNLLPCDLNYYVKGTSIKGN---------IKPGKEAVLHAADTTQNIELGVLLENFPVCKELLIPSGTQ-NYVVRMRLYDTNKRLLCLIIRIILRADGALKILISAPYWLLNKTGLPLIFRQDN-SKTDAAGQFEEHELARSLSPLLFCYTDKEQPLMCTMRVGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQPGNRPGLIYNIGINVRKGKGRYQDTHIVTFAPRYLLDNQSSHKLAFSQREFARGKGTANPEGYISTLPGSSVVFHWPRNDYDQLLCVRLMDV---PNCTWSGGFEVNKPKSFHVNMRDTLGTC-YFLLAEIALKGATYHISFSDTDQLPSPFRIDNMSEVPIQFWQ-YEVPDIRLHTEVKAGAVQDYACDEPILPPYLTLTVKGAGSSEVTADMNCFREYNKLYYENFIYIAATFTFSQDASKRPGAMKHDVSCAELVLDVETKTQRVILKKKEPGKRSQLWRMSGKGMLCHEGSSPPQS-KPAQPRPLDYYMVLDIAGLAAVTDNSYEPL---MLKRPDPRRSTTQTWHFCSGMLTCGLPRLVVQVNGGV--SGLYDGAEVVLGPDSGLLDKPPEQQFINQKMRPGSGVLSVQVLPDGPTRVLQISDFNQRRINRTSPSTEENQNKENTQEKDTEEELEVLINLEDGVGLSLVNKVPEELVFATLSGINLHFTRTAASQVLELSVKTIQVDNQLLGTTQPVMLCLTPNSTDSSVVVDSGPALQVNAVKVPSSLMLTELFKHLMVTARRFTVIIEEKLLLKLLAFFGYGKTEGEVEKLDENLYEKPSEEAGPRKRYYFENLKISLPQVKLSVFTSHKLPPDLKTLKRTLGIPLIRFEDAVINMYPFTRVHPYETQEIIINDILKHFREELISQAAQILGSVDFLGNPMGLLNDVTEGMSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDHRHQSEREYIRYHGATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVTGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSNHRLSVQRVRKPRCCKGPQGLLP 4212
BLAST of Vacuolar protein sorting 13 homolog D vs. TrEMBL
Match: A0A3B3DNX5 (Vacuolar protein sorting 13 homolog D OS=Oryzias melastigma OX=30732 PE=4 SV=1) HSP 1 Score: 1103.2 bits (2852), Expect = 0.000e+0 Identity = 1142/4366 (26.16%), Postives = 1918/4366 (43.93%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS-NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR-------ERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIP--AP----QQSG----------W---WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLE-GIKLKLLKDTEIPIL--------EVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-----------NPDYKNDSFRYVVSSREHSKVPG-GEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLE-------------NLSMATDCQASKETKPVTNIYDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTN--------FMN---NYRNAD---------SSSVKYKKQTRMDNLNKSP-EINQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLSE------------------------------SIG------------DPIIEFTYK-ISQKSGSSDIQRQINLI---FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKEFD------------VSIACKAVSILLIKISS---NLASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGS---NETKME--------ISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILS--SEESTRIGFLN---------------------------------SVKLHLRM--LSPILALPRHKTSNQIILVNLGDIEITS-------DTQSLQVAFSDAIVIS---------------------------KELPIENG--------GYDIGICTDNNAKF-----PLVNKIDLMLKFSK--LHDPPKILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDEIPKPFIE--------------FEDENMQEDQNPDPSEN--------------EIPIVLQSNISQIKMELYPDSFD--------SSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIPPPSP---------FYKYSKRKKSLSNS----VDIQNCAAFELKLPKNTVKSTEP----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYD--------PKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEF-------KFENNDR----------------------------------------SRCLLDEMKIDLHEMDVYSAIRITDSILKDG------DLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANK----RLNVFPIIISPDK-NTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISF-------QPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRE-NRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQ-------------NQTIPPAN------------------------------LAYQTGYL--RHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGN-SKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF--QERNV-----------------AFCDCR---KRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKP---NTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP---LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQ--YNCNEDQNKP---IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETF----KEFYAPLVYKNW----FRPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSV--------EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEE--KMLLKTMQMFL------------------SPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLLP 11344
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L + F+ P VK+G IG++TL+IP+ R S W I + + +I GP EKE ++EN KK LL ++E K++ + Q+G++ W S ASL RI+ N+++++++VH+R+ED+S+ + F+ G+ I+ S ++ E K + KQ+++ S+Y DT+C+++ ++ I+ + + I P+ +++RN + PL PRI +Q+E + + LSQ QY + E +R +R + K+RP+V ++ + ++WW+FAI E ++ + R +RAR+ Y Y L S LE +E + + L LRE+ + R+ D + +V AP Q+SG W WG +G +SE E+ LP S+ +D+ + L D +N FTR D +F R EF LE G + +D P+L E + ++V ES P+ S + + L +LF+RDL +P + + + RE S+V +F + +E NP S +R L + + PL I++ P I + E+F + + + AA + K +T I++ +++ + RW + L I AP++I P+ ++ +P V++DLGR+ ++N D S K PE + D TP SP L+ +L + QA +Y++Y + ++ +VG NW L E E+ ++E FN +L L+++ Q P ++ + L + I+ +K+ + CL +++ FM N R+ D K Q NL +S + Q+ + V + F + + +ES R ++ L + T + +S+ + L+L D GS D ++ASHK L D P SIG D +I+ Y+ +S S ++ + + ++LD+I N ET+ ELL F++ F + + P +E D ++I +++LL++ S +L +E R ++ I+ +++N+ M G+ + + + S+ + D++ +++ ++ GS N + ++ D +N ++ E + G + + + Y HS +FL + +E + F + N A T ++S +E S + SVKL+L + SP++++PR ++++ +LG I I + ++LQV D + S +E P G I D +F PL L L ++ ++ I+ EF + + + K VYEQVL+++DNL +E F D PS N + P + S+ +Q+K+ L+ ++ + Q + +F + + ++LA+ + + +L+ K K L+ S + + +E S SCP S SN +P P F Y + + SNS C + P + S +P + L I + L+D+ F T S+ R VD DFN L I+++ WV++LDFFG+GS + P+ V D+ P ++ D E + +NTK+++ + SL+L + +N A VS + L+ DG+M+L GSL + DL+P G++Y +R F D ++ F++ KYG PDP L RE D+ + +++ + Y+HTQRF EL F +F Q QD L R G+ V S QR++R+ + I AP++++P S+ S +++ + G+ + N F F D+ CLLD M +DL EMD+++A R + G D+ F +R L + C L L V RNL+ + H PD I+G LSSV S+ + Y+ IRG+L +NLGE + L+ Y Q DP +T L+G Y + + + DV L L+ + + L R +F +SKL +ESFS+ SK++ LV + D RYS P + R NVF I+ P K T R+ + QL + Y S + + F V LN ++ +IFDW+Q+ ++++ P S + R+ + S +S +++ V+ ++ + Q+ E+ +NV + F ++E+ S L ++A+IL +YK R +RP FS S++ + SC + S+ LSII+PV + + +C G P+ L D + ++ L ++I A IRLS++DI L I +SF + + PP++ L + G+L W N +NV + P+ + S + + I E + +C IDDC+D D+PL ++ F+ + V +G+ +G LS DYYN GW P IEPWP + W K + S+ L+ +ISS LL+ K T W ++ +E + +F + FVP+ + N GC + + L+ + ++ + V DS S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+GIF+R+ D + +V + I R+V I +GS + I +RS L + N L++ +E + + S+ VVL P + IPL + +Q RP LF+ + + W +V P + ++ C S+ ++ S ++N + + I++ N+P A + V PGH I + L N LPC++ + +K S+ + EA++ Q+ V L+SFP C LLI + ++ V +RLYD + RLL L +++ R G + ++IS+ YW++N+T LPL++ Q +G+++ AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRS+ L F Q + + + P I LP S + FHWPR+D DQLL V+ +D P SGGF+++ SFH+ +R N LR ITL+GA Y++ FT+++ LP P +++NL+ + + F+Q+ + R H+ + P S + YA DEPTLPP + + ++G S D+N LYY+NF+YIA ++F + LV DV ++V L +K RSQLW + L HEGS P + K R D+ L+LD+ LA + S ++L + D RR Q W+F+ G + + + A ++ G E AP + + RPGSG + + V+ DGPTRV+++ D + S + +S+ + + H EG G+ L+N +PEEL++A VD+ + R + L + + IQVDNQ + + ++ +P SS + + E G V+ P +IL E K L+ T Q F E E+L+E S+ + + G ++ Y NLK+ I LSV T++ LP DLK +K FP L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D+T G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ S+ T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A SS R +PRC LLP
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRE-FDLPYEVKTGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPTPPQEYDEEKEREDENERKKRLLKALEDKFKCECEQRGES------YWYSVTASLVTRIVENIELKIQDVHLRFEDDSSNLEKPFSFGVCINNVSAQNPTKEMVQKL-------LRQKQLEIEDFSVYWDTECEMLEKLPANQIQDAMTRCMQNR-DHQYIFEPVCASVLVRRNASKEPLRSRSTPRIEGLVQLEPMSLRLSQVQYLQIMTFLKELDRRERGMLYRKWRPKVPILSNCRQWWMFAI-EANLNVIREQRRRCSWMFSLQRARDARTYTSLYFTRLKGISLSPLEESELKRVEEEQTFEELHSLRELVYDWF-RQQEDSTEPSVECQPDAPVTTIQKSGSSGMIQYLQSWFPGWGGWYG----------------DSENLVRSEEPLPSPSSWDILAETEDLFDPLE--DSHTLNTFTRRDHMFARLEFLLEKGTIMLFEQDRRGPVLHESGVIQLEFSVVKVVV--ESLPRSESSLLSVKLGNLFLRDLTTQGSIFPVLMSPKTVREIWFNIHFQREKSRVESTSPVFEMIYERNPAKS----KFERRLEVRTSPLNIIYNPQAIKKVTEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENGKRWTMKLDICAPQVIFPD-DFQSGDPVLVVVDLGRILLTNSQDDSAVSS-KSAGPEGEDMSDDEYQTPLATPPESPPPELDLPLKVQLKNPEVPSLKIPEAAQAYSR-----KLYEKYCLSFMDLQIMVGRYKDNWKHLQEGEVGPTH-------VVEKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHINLEKMTALQSCLTRLSSPAPSETDKFMERDPNVRSPDPVPQDKFFFRGDSSLKLQGSAKNLTQSVMTLEQHTREVLVESRLLLAEFKVDYMQLGVESEGRYISVLKVFGTNAHFVKRPYDAEVSLTVHGLLLVDTMQTYGSDFDLLVASHKHLGFDLPTGSLKESQPSSPDLCNNSSSEHQETLPELSSNISIGNTSPFSSILKDEDALIKLEYQFVSSDCPSMNLDSSLQVTSVQVNNLDIILNPETIVELLKFLQKSFPKEDSSTPSSVQQHPTHLHDEEEDRTFQATYDQNKELTIEIHRLNLLLLRTVSTGTSLGAEKRGLKIATASIAGTKVNVSM---GSTFDVSGSLGSMQLGDLT--QEGNRSQFVVSIGSIEGNSSDLDGLTFLSDASGSQTDALNFRLL-EQSRGECSLKLHMASLHYNHSAKFLRELSLSANELEDNFRSMLKNAATKVSTVLVSKTAEYSGMVSLFETPLKKSRTFSHSWPFEEEEEIAVEEPESSLNDFSVKLNLNINIESPVVSIPRKFGHPELLVGHLGSIRIRNLLNSQDLPGETLQVLVKDIRLYSLNMSHLVPKRAPRGEKADGVSRSCTNQEEAPFTRNDFFESLSRGQAFHILKDTTIQFTVEKLPLDEDAQLSLNTTEESYTSMGQLRIQGEFVTPVQVFLCKPVYEQVLQTLDNLFLIEEQHTMPSQPPTPPPPTPSSVRLHCFPDPQGGLFARDPPSVNFSHSSLSSPLSLQSQKPALYSSSFTQLKVNLHVSELQVQLSADLTQGSQGLVSVRFQDLEGDFTKDHPNSLRVQLALRAVLMEDLLEQNPKSKYKYLMVSRGAPKPSTFSPKEYLSQSCP--------SSSNAFYPDMPRSLPAHMEEAQNVFQLYQRHSSTPSNSNRKSKKDPECPSTPPPSPLHRKASLQPSDFDDSLVHINVLLVDQNHPEFKTRYGSVGRSVDIDFNCLDVLITLQTWVVILDFFGIGSTASNHAVKEETPPQAVPDV-------PLYDPDGD-EAVQPVNTKIDLKVHSLSLVLNKK---LNELARASVSNLSAHLEML--DGDMSLHGSLRSLSMSDLTPHGTLYGER-FTTQGGDALV-FNILKYGQPDPHLERECDIMVSLQMA-SVQYVHTQRFQAELVAFIHHFTQLQDILGRQRAAMEGQTVRDSPQRASRVLLDIEAGAPVIVIPESSQSPRVIVANFGQLRVKNRFLPAGASGTFSLRDKVEKLTMWTMFSTSIVSAAFWSCGHRVSGAFRGGIPEPGAEHSCLLDCMALDLQEMDIFAAERFPCCLWDIGSNTESSDVVFPSYFVRRTGDNLLKDCCRLRLKVERNLDRELCHAVPDMSIHGSLSSVHCSLDRKHYQLIRGLLENNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVYTNLSFLMNMMDVSLELLESSSHTQHRGS--LGRFDFVKSKLLFESFSNGSKSVNLVSHSLLANDTRYSE-PNRRADGSRPNVFDCILQPSKTGTNRA-----SLQLELHYRSTRESSCFTVVLNNLRVFLIFDWLQLVRDFL-QMPDEKRSGGSKSRQRWSSY------SSTESGALATVMPKTVKTGVVTKRSTVPVTQDHC--LEVKINVTGTEFVVVEDRSSLESNAIILKGTTVLTYKPRLLDRP--FSGSLAGVEVFSCRLGSEQETALSIIDPVNVQVD----LC---GSPTYQTSSGLLDAFNVEDIPPL-LEIQFPALDIRLSYNDILLFLAIAKSFPTASSILSPDTDRVDASPPPSDPFSQKTSTISETQLSHLLDLGFRREDCKRALIHCKGHLDLAATWLLENAENVVGHSRTTPD-SGSSSHSAPLSGVEI----KAENLCICFIDDCLDCDIPLAELTFSRLCVLQRIGSVQEGNANFTLSGDYYNRELSGWEPFIEPWPCFLSWQQQAAGRLHPPRFKMGIRSKQRLDVNISSVLLEQYKTTKSSWMADYCREEEHTPSSSSSPPWMGSSVEPPSFGQSELLMSKRRQPFVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISDIHNSGVDDSHNVSQWREVLPGEELPFEFEAREKLRHRH--THELKLHQLLVRVCGWEQVKPVSVDKVGIFFRYAAPDRNSSSSIVGSPISRTNIIHPHVYFSALPPVRVVFAITMEGSARKVITVRSSLMVKNRLDVLME-VRLDSPSAPDKPVVLPPILPGQALAIPLHLTSWRLQARPKGMG------------LFFCKVPVH-----WTSVERPDEISSSKRECQSA--DYDDSHKQNFRFCV-VIKKENYPDQLPAKRQVSGGTQEIYRQPGHTIYLLPTMVLVNLLPCELNYYIKGVHIKGSLNS-GKEAVLH--------LADTSQNLELGVLLESFPVCKELLIPPGTQ-NYVVRMRLYDTEKRLLCLTIRIILRAQGALKILISAPYWLINKTGLPLIFRQDNGKSD--AAGQFEEHELARSLSPLLFCYTDKEQPAMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGISVRKGKGQYRDTHIVTFAPRYLLDNRSTHKLAFAQREFARGKGASNPDGYISTLPGSSVVFHWPRNDYDQLLCVRFMDV---PNCTWSGGFEVNKPKSFHINMRDTLGNC-FFLRTEITLKGATYQISFTNSEQLPPPLRIDNLSEVPIQFWQH-GVADLRLHTEVKPMSVLDYACDEPTLPPYLTVTVKGAGSSDVTADMNFFKEFNKLYYENFIYIAATHTFSQTAETRPVGKKRLMSCLELVLDVDTKTQRVILRKKEPGKRSQLWRMTGTGMLCHEGSSPPQN-KPTQPRPLDSSLVLDIAGLA---AVSDNSFEPLMLRRPDSRRSTTQTWYFSSGMLTCGLPRLVVQVKGGI--AGLYDGAEVVLGPDSGLAAPGPEQQFINQKMRPGSGVLSIQVIPDGPTRVLQISDFNQRRTIRSSASNEPEESTEEAKSKSHKQELEVMVNLEEGLGISLVNKLPEELVFATLFGVDIHFTRTVTNEVLELSVQNIQVDNQLLGSTQPVML-----CVTPHSSDSNLN-----ESGPALQVNSVK------VPSSLILTELFKHLMVTAQRFTIIIEEKLLLKLLSFFGYGEAEAELEMLDE---SVPERPSEEAGPPKRYYFENLKISLPQIKLSVFTSQKLPPDLKALKGTLGFP-LVRFEDAVINMYPFTRVHPYETQEIIFNDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHGATSGEH---LVAGIHGLAHGIIGGMTSIITSTVEGVKMEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSSHRLGVQRVRKPRCCKGPQDLLP 4167
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Cavefish
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:ZFIN;Acc:ZDB-GENE-050912-1]) HSP 1 Score: 671.774 bits (1732), Expect = 0.000e+0 Identity = 576/2007 (28.70%), Postives = 930/2007 (46.34%), Query Frame = 2
Query: 5930 YANINLC---RLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYS--SFPANKRLNVFPIIISPDK-NTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWI-FTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISF-----QPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQ------------NQTIP----PANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKLS--------------KFVDRISIHHLDDYEFVS----------------------------VCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERN-----------------------VAFCDCR-KRHNNLFVPFLIVNELGCPIEYRKL-------SLDNPPMYNEIWNANKCGDEN-----KWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLK---PNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP--LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEG----GILVNKLGMKCYINASCKNA-AVFGGFETFKEFYAPLVYKNW-FRPGSGEVLVSVVTDGPTRVM-------RLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYA---CVMYEENKS----ESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMF-LSPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMK-KLFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRY-EPRCLSSSWKLLP 11344
++++ +C R +F +SKL +ESFS+ SK++ LV + D R S S + NVF I+ P + T RS + QL + Y S D + F V LN ++ +IFDW+Q+ ++++ A G A R+ S P S V+ ++ + Q+++ E LNV + F ++E+SS L T+A+IL +YK R +R PFS S++ + SC + S+ LSII+PV + + ++C G P+ L D + ++ L ++I A IRLS++D+QL I +S +QT P P + RHR +L + LT LQ L +D+ + L++ E +S +C IDDC+D DVPL ++ F+ ++V +G+++ +F LS DYYN GW P IEPWP + W K + ++ L+ +I+S LL+ T W ++ + + D + + F+P+ + N GC + + L +L + + I + + G ++ +W V F+ AR+K+R+ +H H++ +++ GW + +PV+VDK+G+F+R+ D ++ + V + I R+V I +GS + I ++S L + N L +P+E + + S+ VVL P + IPL + +Q RP LF+ + + W +V PG+ ++ C S + F+ Q K I++ ++P K V PGH I + L N LPCD+ + +K + +M +P + + Q+ + L++F C LLI + ++ V +RLYD RLL L +++ R G + ++IS+ YW++N+T LPL++ Q +G+ + AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDN+SS L F Q + + I LP S + FHWPR+D DQLL V+ +D + SGGF+++ SFHV +R N LR ITL+GA Y++ F+DAD LP P++++N++ + + F+Q+ R H+ + + + YA DEPTL P + L ++G S D+N LYY+NF+YIA +F D LV DV P ++V L +K RSQLW + L HEGS P K R D+ L+LD+ LA +S + ++L + D RR Q W F+ G G+ + +K ++ A V G + + + N RPGSG + V V+ DGPTRV+ R I++ S + + + +G G+ L+N +PEEL++A ++V + R + L + + IQVDNQ + CV ++S P+ NA + L +F + + +I+EEK+LLK + F ++E E L+E+ L ++ D G ++ Y NLK+ + LSV T+ LP DL +K L L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNPIG ++D+T G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ SV T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A S +R +PRC LLP
Sbjct: 2193 FSDLCICSLHRFDFMKSKLLFESFSNGSKSVNLVSHSLLAYDTRNSGPSKATEGKHNVFDCILQPSRTGTNRS-----SLQLELHYRSTRDSSCFTVVLNNLRVFLIFDWLQLVRDFLHMPAEKG----AAPSRQRWPSG-----SSDPGSTGAVMPKTVKSGVVTKRSTVPVTQDKY--LEFKLNVTGTEFVVVEDSSCLDTNAIILKGTTVLTYKPRLLDR--PFSGSLAGIEVFSCRLGSEQETALSIIDPVNVQV----ELC---GSPTYQSSSGLLDAFNIEDFPPL-LEIQFPALDIRLSYNDVQLFLAIAKSIPTGSPSITDPASVSQTAPSTASPDTSSKPKDSFRHRTEAL----------LEGQLTHLQDLGFRKEDCRRALILCRGQLDQAATWLLENAEAMSGRSRSNSESGSHSATPLSGVEVKAESVCICFIDDCLDRDVPLAELTFSRLSVLQRIGSTQEGNASFTLSGDYYNRELSGWEPFIEPWPCVLNWQQQAAGRLHPPRLKMGVRAKQRLDINITSVLLEQYSTTKTSWLADYCKEDEQSPQSSSSSSLSWMGSSVDPPSFGQTDVKLSKRRQPFIPYALRNHTGCTMWFATLTTTPTRVALSHSGSADSISDVHGPGTDDTHNISQWREVLPGEEIPFEFE----AREKLRHRH--THELKLHQLLVRVGGWEQVKPVSVDKVGVFFRYAAPDRNNPSNTVGSPISRTNIIHPHVYFSALPPVRVVFSITMEGSARKVITVQSALMVKNRLEVPME-VRLDSPSAPDKPVVLPPILPGEALAIPLHLTSWRLQARPKGLG------------LFFCKVPI-----HWTSVERPGEISSSKRECQS--ADFD-DQLKRSFRFCVVIKKEHYPDQQPAKTVSGGAKQIYRQPGHTIYLLPTLVLANLLPCDLNYYIKGTSIKGTM---------KPGKEAVLHAADTSQNLELGILLENFTVCKELLIPPGTQ-NYVVRMRLYDTNRRLLCLTIRIVLRAQGALKILISAPYWLLNKTGLPLIFRQDNGKMD--AAGQFEEHELARSLSPLLFCYTDKEQPTMCTMRVGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQPGNRPGLIYNIGINVRKGKGRYQDTHIVTFAPRYLLDNQSSHKLAFSQREFAKGKGTANPDGYISTLPGSSVVFHWPRNDYDQLLCVRLMDVTN---CTWSGGFEVNKPKSFHVNMRDTLGN-CFFLRAEITLKGATYQISFSDADQLPPPFRIDNISEVPIQFWQH-GVPDIRLHTEVKAGAVLDYACDEPTLSPYLTLTVKGAGSSEVTADMNFFREYNKLYYENFIYIAATFTFSQDASKRPGVIKRVASSAELVLDVEPKTQRVILKKKEPGKRSQLWRMTGKGMLCHEGSSPPQS-KPAQPRPLDSSLVLDIAGLAAVTDNSYEP---LMLRRPDSRRSTTQTWHFSSGMLTCGLPRLVVQVKGGVSGLYDGAEVVLGPTQGLLDLLPEQQFINQKMRPGSGVLSVQVLPDGPTRVLQISDFNQRRINRTSPSTEEDPSKEDQKKTVEEELELLVNLEDGVGLSLVNKVPEELVFATLTGINVHFTRTAASKMLELSVQNIQVDNQLLGTTQPVMLCVTPGSSESTVADSGPALQVNAVKVPSSLML-----TELFKHLMVTARRFTVIIEEKLLLKILAFFGYGQTEEVEKLDEN---LYEKASEDAGPPKRYYFENLKISLPQVKLSVFTSHKLPPDLMALKGTLGTPLIKFEDAVINMYPFTRVHPYETQEIIINDILKHFR-ELLSQAAQILGSVDFLGNPIGLLNDVTEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSEREYIRYHGATSGEH---LVAGIHGLAHGIIGGLTSVITSTVEGVKTEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSNHRLGVQRVRKPRCCKGPQGLLP 4094 HSP 2 Score: 307.76 bits (787), Expect = 8.947e-83 Identity = 335/1485 (22.56%), Postives = 644/1485 (43.37%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFE-KEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVL-NKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPA---------------------------PQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEV-DFEAVNFFT-RDKIFLRGEFSLEGIKLKLLKD-------TEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKV----PGGE-------------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSP-------FGSLENLSMATDCQASKETKPVTN-IYDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKK-----------QTRMDNLNKSP-EINQNYKSVSI-------SFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSES-----------------IGDP----------------IIEFTYK-ISQKSGSSDIQRQI---NLIFSDLDVIANQETLTELLSFMRLLFSNDSLQIF------------DQKPETPNTTEFKEFDVSIACKAVSILLIKISSN---LASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTL--------TTGSNETKMEISE--NEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAIL-----------------------------------------SSEESTRIGFLNSVKLHLRMLSPILALPRHKTSNQIILVNLGDIEIT-------SDTQSLQVAFSD 3790
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L + F+ P VK+G IG++ L+IP+ R S W I + + +I GP + E +E + E +KK LL ++E KW+ + Q+G++ W S AS+ RI+ N++++++ VH+R+EDN + P + FA G+ I S ++ + EL ++ KQ+++ S+Y DTKC ++ + I+ + + + I P+ +++RN + PL PRI +Q+E + + LSQ QY+ +A E +R +R+ K+RP+V V + ++WW+FAI+ E R AL +RAR+ Y Y L + + EF +E + L+ L LRE+ + RK ++ ++ + P GW+G R + P + ++L+P T++ + + +L + + D + +N FT RD +F R +F LE + L + +E ++++ + +G ES P+ S + + L LF+RDL + F +VS + V P G+ +F + +E NP+ S +R L +++ PL I++ P I + ++F + + + AA + K +T I++ +++ + RW + L I AP++I P+ ++ +P V++DLGR+ ++N D + +S K T E +L E++ TP ++P F E + ++ + ++ + +Y++YS+ ++ +VG NW L ESE+ ++E FN +L L+++ Q P ++ + L + I+ +K+ + CL ++ + R+ D +++++K Q NL KS + Q+ + V + F ++ + +ESG R ++ L + T + +++ + L+L D GS D ++ASHK L D P L ES +G P +I+ Y+ +S S ++ + L ++LD+I N ET+ ELL F++ F + + ET +T + ++++ +++LL++ S + E R ++ I+ +++N+ M G+ L+ + I + D++ + ++++ T ++E + S + + +N ++ E + G + + + Y HS +FL + +E + F + A T + + EE FL + L++ + SP++++PR ++++ +LG I I S+ + LQV D
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSVALLKGAVELENLPLRKDALRE-FDLPFEVKAGFIGKIVLQIPFYRPHSDPWLISMSQLNLIIGPAHPQEYDEAQEKEAERELKKQLLKALEDKWKSECEQKGES------YWYSVTASVVTRIVENIELKIQGVHLRFEDNFSNPEKPFAFGVCIKNVSAQNP----------SKELKLMRQKQLEIEEFSVYWDTKCTMLGNLPPAEIQESMTACMQSR-EHQYIFEPVCASVLLRRNASKEPLRSRSTPRIEGQVQLEPLSLHLSQVQYQQIMAFLKELDRKEREMLFRKWRPKVPVSGNCRQWWIFAISANLDEIREKRRRGNWDFAL-QRARDAQLYTSLYRQRLKGVTLSPQEEF-ERIEDEQTLEELQSLREIVHDSF-RKQEEIAESVREPMSEPPASSPSSSIPRSGSSGMIQYLQSWFPGWGGWYG--GAERGPDGQP--------------VTDELMP---GTAQWDILTETDDLFDPLEDSQTLNTFTRRDHLFARLDFLLEKAAVTLFQQDKLNPLSSESGVIQLEFSGVKIGIESLPRSESSLLSVKLGGLFLRDLT--TQGSIFPVLVSPKPVVAVAINQPFGQSSSTDKSNSSASPVFEMIYERNPIRS----KFERRLEVNTSPLNIIYNPQAIKKVADFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKRWTMKLDICAPQVIFPD-DFQSDDPMLVVVDLGRILLTNSQDDLQTKS-KATPSE----GDELSDEEYQTPLATPPPESETDFKEQEKTVDLSSLKSVEGSQSFSRMLYEKYSLSFNDLQIMVGRYKDNWKHLQESEVGPTH-------VVEKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHINLEKMTALRSCLARLST--ESLRSPDPLTLRHEKIFQREDSQWKLQGSDKNLTKSVMTLEQHTREVLVESRLLLAEFNINYMQLGVESGGRYISVLKVFGTNAHFVKRPYDAEVALTVHGLLLVDTLQTYGSDFDLLVASHKHLSFDVPTGSLRESQPTSPVSQDGKSPEPSELGMPFDKVSPLSSFLRDQEVLIKLEYQFVSSDCPSMNLDSSLQVTTLQVNNLDIILNPETIVELLKFLQQSFPKEESSWTPPTPQPPPLPNGQGQDETYQSTYDQNTELTVEIHRLNLLLLRTVSTGTVMGGEKRGMKIATASINGTKVNVSM---GSRLDINGSLGCIQLLDLTQESGRGQFVVSIGNSEDDPSATKASELTIPTSALTSSEALNFRLL-EKSQGECSLELHMASLHYNHSAKFLKELSLSANEVEDNFRSMLKTAATKVSTVLATKTAEYSGMVSLFETPSRRSRSHSQCNWTLDPFEDEMDLIDAKEEPGLDSFLVKLTLNVSIESPVVSIPRKPGHPELLVGHLGSITIQNFVAGQDSEGEKLQVEIKD 1420 HSP 3 Score: 162.54 bits (410), Expect = 7.142e-39 Identity = 169/663 (25.49%), Postives = 280/663 (42.23%), Query Frame = 2
Query: 3980 KAVYEQVLKSMDNLVYDDEDEI--------------------PKPFIEFEDENMQEDQNPDPSENEIP---------IVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPD------------LLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNSVDIQNCAAFELKLPKNTVKSTEP----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSR--------------------FYD--PKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEF-------KFENNDRSR-------------------------------CLLDEMKIDLHEMDVYSAIRIT----DSILK--DGDLKFSYLNIRPINPKPLSEKCNLNLTV 5632
K VYEQVL+++DNL ++ + P P P ++ P I + +S+++++L D S+ ++ + Q + +F + + Y ++LA+ + + +L+ + K L+ S + + +E S SCP L Q+E + + P +P K KK D + + +NT P + L I + L+DK+ F T S+ R +D DFN L I+++ WV++LDFFG+GS Y+ P D+ D+ R +NTK+++ + +L+L + I A VS + L+ DG++ L GSL L+DL+P G +Y +R + L F++ KYG PDP L RE DM++ +++ + Y+HTQRF E+ F Q+F Q QD L R G+ V + QR++R+ + I AP+LL+P S+ S +++ +LG+ + N F F D+ CLLD + +DL EMD+++A R+ DS K D DL F+ ++R L E+C L L V
Sbjct: 1531 KPVYEQVLQTLDNLSVIEDQRVSPSQPPTPPPPTPSSTKPHHPPLIGLSYSSISSSLSTPLPLQSTSPPLQPPSFTQIRINFKVSELQVQLSADLSQGSQG-LVSVRFQDLEGDFAKDHPYSLSVQLALRSLLMEDLLEQNPESKHKHLMVSRGAPKPSTFSPKEYLSQSCPSASNAMYPDMPRSLPAQIEEAQNVFQFYQRHPSTPSASSRKSKK------DPECPSTPPPSPSRNTPSPHPPPDFDDSLVHINVLLVDKRHPEFKTRYGSVGRSIDVDFNCLDVLITLQTWVVILDFFGIGSTANNHAVKVPTTSPQPMPGHPLYNTAPTDLSDMCSEMNEEEMEER---------VNTKIDLKVHSLTLVLNKK---ITELAKASVSKLFTHLEMI--DGDLALQGSLGSLSLIDLTPHGDLYRERF--TTRGGEALIFNIHKYGEPDPYLERECDMKVSLQMA-SVQYVHTQRFQTEVVAFIQHFTQLQDVLGRQRAAVEGQAVRERPQRASRMLLDIEAGAPVLLIPESSRSKRLIVANLGQLKVSNRFLPAGTHGTFSLRDKVEKLTMAHTFFPSLVMMFLMLCNLCPCAVEDHVCLLDCIALDLEEMDLFAAERLPCEPWDSGGKHVDTDLVFTSYSVRRTGGSLLKERCRLKLKV 2169
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Cavefish
Match: vps13a (vacuolar protein sorting 13 homolog A [Source:ZFIN;Acc:ZDB-GENE-030926-1]) HSP 1 Score: 150.214 bits (378), Expect = 3.951e-35 Identity = 190/879 (21.62%), Postives = 372/879 (42.32%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKL---NEMPKS-DCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVK-YVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLW------GRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIP-ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSR---EHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYF----SRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALV----GNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLE 2560
++ L+ +L+R +G YV N + + G++ GD L NL +K L L + P V++G IGR+ L+IP+ +Q+ ++++Y++ P ++ K EKE + ++ L IE +K Q+ N K E + ++V L ++++NLQ+++ N+HVRYED+ T P+ + G+ + S +++D W+ L + + K V L + Y + ++ S + LQ+ + N +P + I PIS + ++ N P++ L + + +V + LS+ QY + L + R + KYRP V V + WW + I+ + H++ +R R R L K Y + Y+ + +K + + + + E L++ + R+ A + + + V + Q GW+G++W +KE P D +E +++ + SE + ++ + + ++ F L + + + + E P I+ ++ L RP + I L + V L +++ ++SSR E +K + +L FETNPL + + L + S+PL I++ + ++ +F + + A + + RTS + I+E + +++ + A +I+P + +LDLG Q+S+ S K P+ + S +E ++ YD + I+L ++ L G+W + E + L ++EP + + + V+ S+ P M + L + ISD K+ +G LE
Sbjct: 1 MVFESLVVDVLNRFLGDYVVNLDSSQLNLGIWGGDAILRNLEIKENALSQL-DIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQE--NPKTEKQD--TFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPLSFGVSLQNLSLQTADANWKP-CLLDEKAKLFFKLVRLDNLFAYWNVNSELYSNHTPPEALRRLQQNVAVQNSIPHDYNFIFRPISANAKLRMNPRSDVDFSSPKVDLVVNLPEVAVELSRPQYVSILELLGSVDMMSRNLPYRKYRPCVPVHNNAYNWWRYVISSVLEVNVKPRLHMWSWQHIR-RHRQLAKQYKELYKTKITSKKPTEVVLKKLEDTEKTLDIFNITLARQQAEMEASKAGLRIYRPGVKMEEEQSQGWFGWMWNWSGDGSTPAKEAKPGGFDTLMTPAEKAKLYTAI-----GYSETAVNPNLPKSFED---------------MKVRFQLVSMSVSIKESREKPEIIRLSVIGLSATLTQRPSAQAIKIIAQLSTFEVTGLP---RSNVAPMLLSSRRVVEENK----PLLSLMFETNPLDAKA----DQRLHVESQPLEIIYDATTVNSLSAFFMPPNDLQLDELTNATRMKLEQFRDRTSTGLMYIIE---TQKVLDLNINLMASYVIVPQMGFYDPSHNLAILDLGHFQMSSQS--------KANLPQLLAGSSTIED------------------------------IMSRAYDSFDIQLSSLQFLYTKPDGDWKKARKE------RQSALHILEPVDLKVVFSRAMVV-TDSRMPKFKMSGELPLLSLRISDDKV---RGVLE 790 HSP 2 Score: 93.5893 bits (231), Expect = 4.918e-18 Identity = 60/224 (26.79%), Postives = 105/224 (46.88%), Query Frame = 2
Query: 10730 FETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSD--QDVYNMVKHFAHGIG---------------DSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPYQ 11350
F T + + HY Q Q + +D LGNP G I ++ G++ + + Q + F G+ + ++I + ++ ++ +TMD EY++KR+ N + SG + G K L G GI + T+ KG + EG +G F G KG+VG VT+P GG++D+A+ + + A +S++ + R PR + + PY+
Sbjct: 2795 FRTTQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAM-NKQPSGLKEGLTRGG-KGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRAAETSQDVESLR--PPRFIHEDGVIRPYK 3014 HSP 3 Score: 64.6994 bits (156), Expect = 2.792e-9 Identity = 46/188 (24.47%), Postives = 95/188 (50.53%), Query Frame = 2
Query: 5252 QDRLSHIRVTSAGRI---VDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF---ENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYK 5797
++ L+ + V +A + V + A++S RI + + +AP++ LP S+ S ++++ DLG ++ N F+ ++ + +LD M + L ++ +Y + KD + ++P+ N++L++ RNL+ + H+ PD QI L + +S E + L+ NL E +D + + K
Sbjct: 1174 KEALAEVTVQAAEKAATGVLELAEKSTRISLDVDIKAPVVFLPQSSISTNVIVADLGLVTVKNHFEIVPSPSHTKIPPILDVMSVALSDLKMYR------TTFKDEKFQTEIQLLKPV---------NMDLSIKRNLSASWYHSLPDIQINAHLKPMSLILSQEDMIVVLRTLSENLSETSDAVPAPK 1346
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Cavefish
Match: vps13a (vacuolar protein sorting 13 homolog A [Source:ZFIN;Acc:ZDB-GENE-030926-1]) HSP 1 Score: 150.214 bits (378), Expect = 4.129e-35 Identity = 190/879 (21.62%), Postives = 372/879 (42.32%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKL---NEMPKS-DCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAIT-------EHTRHIFKGAALRERARNLVK-YVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLW------GRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIP-ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSR---EHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYF----SRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALV----GNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLE 2560
++ L+ +L+R +G YV N + + G++ GD L NL +K L L + P V++G IGR+ L+IP+ +Q+ ++++Y++ P ++ K EKE + ++ L IE +K Q+ N K E + ++V L ++++NLQ+++ N+HVRYED+ T P+ + G+ + S +++D W+ L + + K V L + Y + ++ S + LQ+ + N +P + I PIS + ++ N P++ L + + +V + LS+ QY + L + R + KYRP V V + WW + I+ + H++ +R R R L K Y + Y+ + +K + + + + E L++ + R+ A + + + V + Q GW+G++W +KE P D +E +++ + SE + ++ + + ++ F L + + + + E P I+ ++ L RP + I L + V L +++ ++SSR E +K + +L FETNPL + + L + S+PL I++ + ++ +F + + A + + RTS + I+E + +++ + A +I+P + +LDLG Q+S+ S K P+ + S +E ++ YD + I+L ++ L G+W + E + L ++EP + + + V+ S+ P M + L + ISD K+ +G LE
Sbjct: 1 MVFESLVVDVLNRFLGDYVVNLDSSQLNLGIWGGDAILRNLEIKENALSQL-DIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQE--NPKTEKQD--TFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPLSFGVSLQNLSLQTADANWKP-CLLDEKAKLFFKLVRLDNLFAYWNVNSELYSNHTPPEALRRLQQNVAVQNSIPHDYNFIFRPISANAKLRMNPRSDVDFSSPKVDLVVNLPEVAVELSRPQYVSILELLGSVDMMSRNLPYRKYRPCVPVHNNAYNWWRYVISSVLEVNVKPRLHMWSWQHIR-RHRQLAKQYKELYKTKITSKKPTEVVLKKLEDTEKTLDIFNITLARQQAEMEASKAGLRIYRPGVKMEEEQSQGWFGWMWNWSGDGSTPAKEAKPGGFDTLMTPAEKAKLYTAI-----GYSETAVNPNLPKSFED---------------MKVRFQLVSMSVSIKESREKPEIIRLSVIGLSATLTQRPSAQAIKIIAQLSTFEVTGLP---RSNVAPMLLSSRRVVEENK----PLLSLMFETNPLDAKA----DQRLHVESQPLEIIYDATTVNSLSAFFMPPNDLQLDELTNATRMKLEQFRDRTSTGLMYIIE---TQKVLDLNINLMASYVIVPQMGFYDPSHNLAILDLGHFQMSSQS--------KANLPQLLAGSSTIED------------------------------IMSRAYDSFDIQLSSLQFLYTKPDGDWKKARKE------RQSALHILEPVDLKVVFSRAMVV-TDSRMPKFKMSGELPLLSLRISDDKV---RGVLE 790 HSP 2 Score: 93.5893 bits (231), Expect = 4.643e-18 Identity = 60/224 (26.79%), Postives = 105/224 (46.88%), Query Frame = 2
Query: 10730 FETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSD--QDVYNMVKHFAHGIG---------------DSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPYQ 11350
F T + + HY Q Q + +D LGNP G I ++ G++ + + Q + F G+ + ++I + ++ ++ +TMD EY++KR+ N + SG + G K L G GI + T+ KG + EG +G F G KG+VG VT+P GG++D+A+ + + A +S++ + R PR + + PY+
Sbjct: 2820 FRTTQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAM-NKQPSGLKEGLTRGG-KGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRAAETSQDVESLR--PPRFIHEDGVIRPYK 3039 HSP 3 Score: 64.6994 bits (156), Expect = 3.195e-9 Identity = 46/188 (24.47%), Postives = 95/188 (50.53%), Query Frame = 2
Query: 5252 QDRLSHIRVTSAGRI---VDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKF---ENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYK 5797
++ L+ + V +A + V + A++S RI + + +AP++ LP S+ S ++++ DLG ++ N F+ ++ + +LD M + L ++ +Y + KD + ++P+ N++L++ RNL+ + H+ PD QI L + +S E + L+ NL E +D + + K
Sbjct: 1174 KEALAEVTVQAAEKAATGVLELAEKSTRISLDVDIKAPVVFLPQSSISTNVIVADLGLVTVKNHFEIVPSPSHTKIPPILDVMSVALSDLKMYR------TTFKDEKFQTEIQLLKPV---------NMDLSIKRNLSASWYHSLPDIQINAHLKPMSLILSQEDMIVVLRTLSENLSETSDAVPAPK 1346
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Cavefish
Match: vps13c (vacuolar protein sorting 13 homolog C [Source:ZFIN;Acc:ZDB-GENE-070426-4]) HSP 1 Score: 129.413 bits (324), Expect = 7.359e-29 Identity = 87/347 (25.07%), Postives = 173/347 (49.86%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLE--DLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWK---LFEKEFQNENMMKKL--LLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVS----VENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAI 1027
+++ L++ LL+R IG YVEN +K G++ G++ LENL +K L F W +G++TL+IP ++A ++ +Y++ P + K + E+ +Q E K+L + +++++ RK+ Q+ D ++ L ++++NLQ++V ++H+RYED+ + PS+ + G+ + S +++D W + L ++ K L + Y + I S V+ +K+ + + ++ I PI M N N P+I+L L+++ + I +++ QY V L + + + + K+RP V V + K+WW++ I
Sbjct: 1 MVLESLVSDLLNRFIGDYVENLDKSQLKIGIWGGNVVLENLRVKENALRVYRCFLW-------FLGKLTLKIPLKNLYTEAVVATLDGLYLLVVPGATIKYDAVKEERYQQEAKQKELQRIEEALQIAARKEKAQEEKKD--------TFAEKLATQVIKNLQVKVTSIHIRYEDDVSDPSRPVSMGVTLSELSLQTTDESWRACI-LNEAAKIIYKLGRLECLCAYWNVNSPIFYRGSWEEIVDKLKAGISTRDEDLKGYQYIFRPIFASAKMCINPNSEIELKSPKINLQLEVQNIAIEMTRPQYLSMVDLLESVDSMVKNAPYRKFRPDVPVHKHAKQWWLYGI 331 HSP 2 Score: 80.8777 bits (198), Expect = 3.750e-14 Identity = 91/386 (23.58%), Postives = 161/386 (41.71%), Query Frame = 2
Query: 10256 IQVDNQQMDAGYACVMYEEN--KSESPSSSQNAF-SLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSK------EAELLEEDFSSLQHQ---------SHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFPSLMSFTNAQIIID-------VYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ-----------------DVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKR-QRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKE 11284
+QVDNQ A + V + + KS + S F + V F + + + M + +++ L +F SP++ + +L++ D +LQ Q S +I +KL +SL ES D+ ++ L L S +D ++ + F + ++ HY Q Q + +D LGNP G I ++ G++ + + V +++ H G ++I S + ++ ITMD EY++KR + + R G + A+ G G GI + P + G K EG +G F G KG+VGVV +P GG++D+A+ + + +A S++E
Sbjct: 2947 LQVDNQLTGAIFPIVFHPVHPPKSIALDSEPKPFIDVSIITRFNEHSQVMQFKYFMVLVQEMAVKIDQGFLGAIFSLFTSPAQSQPDKQKMKLIDRDLEALQAQLMESSITDASGLSFFEHFHISPIKLHL-SLSLGSSGDESEDGDMVAIQSLNLLLKSIGATLTDVDDLIFKLAFFEVKYQFYRREKLMWAVIGHYTEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPFQGAVQGPEEFAEGFVIGVRSLLGHTVGGAAGMVSRITGSVGKGLAAITMDKEYQQKRREEMNRPPRDFGESLAKGGKGLLKGVVGGVTGIVTKPVE---GAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEE 3328 HSP 3 Score: 59.6918 bits (143), Expect = 1.060e-7 Identity = 42/158 (26.58%), Postives = 75/158 (47.47%), Query Frame = 2
Query: 5309 AQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDR--SRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENT 5776
AQ+S R+ + I +APL+++P S+ S + +VDLG ++ N F + +++ M I+L + + Y + RP + L + NL L V RNL + + P ++ G+L S+ S+ E + +LA N+GE +
Sbjct: 1103 AQKSFRLSLNIRLKAPLIIIPQSSTSHNAFVVDLGLITVSNSFSLLPAEGFPLPAVMETMDINLTHLKLCR-----------------YCHQRPQSEVELLQPINLELLVKRNLAASWFTSIPGVEVTGVLKSMNISLGQEDLVVLMSILAENIGEGS 1243
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Cavefish
Match: vps13c (vacuolar protein sorting 13 homolog C [Source:ZFIN;Acc:ZDB-GENE-070426-4]) HSP 1 Score: 119.013 bits (297), Expect = 1.199e-25 Identity = 85/372 (22.85%), Postives = 177/372 (47.58%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLE--DLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-------------EKEFQNENMMKKL--LLDSIEVK---------------WRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVS----VENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAI 1027
+++ L++ LL+R IG YVEN +K G++ G++ LENL +K L F W +G++TL+IP ++A ++ +Y++ P ++ F E+ +Q E K+L + +++++ +R+ + + ++K + + ++ L ++++NLQ++V ++H+RYED+ + PS+ + G+ + S +++D W + L ++ K L + Y + I S V+ +K+ + + ++ I PI M N N P+I+L L+++ + I +++ QY V L + + + + K+RP V V + K+WW++ I
Sbjct: 1 MVLESLVSDLLNRFIGDYVENLDKSQLKIGIWGGNVVLENLRVKENALRVYRCFLW-------FLGKLTLKIPLKNLYTEAVVATLDGLYLLVVPGASEYCFSVFNLSIKYDAVKEERYQQEAKQKELQRIEEALQIAARKDKGRKHKKHKKTFRRCKKHEQKSEKAQEEKKDTFAEKLATQVIKNLQVKVTSIHIRYEDDVSDPSRPVSMGVTLSELSLQTTDESWRACI-LNEAAKIIYKLGRLECLCAYWNVNSPIFYRGSWEEIVDKLKAGISTRDEDLKGYQYIFRPIFASAKMCINPNSEIELKSPKINLQLEVQNIAIEMTRPQYLSMVDLLESVDSMVKNAPYRKFRPDVPVHKHAKQWWLYGI 364 HSP 2 Score: 81.2629 bits (199), Expect = 3.007e-14 Identity = 91/386 (23.58%), Postives = 161/386 (41.71%), Query Frame = 2
Query: 10256 IQVDNQQMDAGYACVMYEEN--KSESPSSSQNAF-SLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSK------EAELLEEDFSSLQHQ---------SHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFPSLMSFTNAQIIID-------VYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ-----------------DVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKR-QRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKE 11284
+QVDNQ A + V + + KS + S F + V F + + + M + +++ L +F SP++ + +L++ D +LQ Q S +I +KL +SL ES D+ ++ L L S +D ++ + F + ++ HY Q Q + +D LGNP G I ++ G++ + + V +++ H G ++I S + ++ ITMD EY++KR + + R G + A+ G G GI + P + G K EG +G F G KG+VGVV +P GG++D+A+ + + +A S++E
Sbjct: 2980 LQVDNQLTGAIFPIVFHPVHPPKSIALDSEPKPFIDVSIITRFNEHSQVMQFKYFMVLVQEMAVKIDQGFLGAIFSLFTSPAQSQPDKQKMKLIDRDLEALQAQLMESSITDASGLSFFEHFHISPIKLHL-SLSLGSSGDESEDGDMVAIQSLNLLLKSIGATLTDVDDLIFKLAFFEVKYQFYRREKLMWAVIGHYTEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPFQGAVQGPEEFAEGFVIGVRSLLGHTVGGAAGMVSRITGSVGKGLAAITMDKEYQQKRREEMNRPPRDFGESLAKGGKGLLKGVVGGVTGIVTKPVE---GAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEE 3361 HSP 3 Score: 59.3066 bits (142), Expect = 1.076e-7 Identity = 42/158 (26.58%), Postives = 75/158 (47.47%), Query Frame = 2
Query: 5309 AQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDR--SRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENT 5776
AQ+S R+ + I +APL+++P S+ S + +VDLG ++ N F + +++ M I+L + + Y + RP + L + NL L V RNL + + P ++ G+L S+ S+ E + +LA N+GE +
Sbjct: 1136 AQKSFRLSLNIRLKAPLIIIPQSSTSHNAFVVDLGLITVSNSFSLLPAEGFPLPAVMETMDINLTHLKLCR-----------------YCHQRPQSEVELLQPINLELLVKRNLAASWFTSIPGVEVTGVLKSMNISLGQEDLVVLMSILAENIGEGS 1276
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003418.1 (pep scaffold:Pmarinus_7.0:GL489749:646:4444:-1 gene:ENSPMAG00000003128.1 transcript:ENSPMAT00000003418.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 47.3654 bits (111), Expect = 2.315e-6 Identity = 32/87 (36.78%), Postives = 50/87 (57.47%), Query Frame = 2
Query: 119 GDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIE 376
GD LENL LK L +L + P VK G IG++TL+IP+ +A +E +Y++ P+++ K EKE + + +K+ L IE
Sbjct: 1 GDAVLENLVLKENALSEL-DVPFKVKVGHIGKLTLKIPWKNLYKEAVIASLEGVYLLVVPSASIKYDAEKEEKQQLELKQRELRRIE 86
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003332.1 (pep scaffold:Pmarinus_7.0:GL487813:5059:8070:-1 gene:ENSPMAG00000003052.1 transcript:ENSPMAT00000003332.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 48.9062 bits (115), Expect = 2.751e-6 Identity = 37/129 (28.68%), Postives = 68/129 (52.71%), Query Frame = 2
Query: 10925 GIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFR----EIARSSKEGQPPR 11299
G ++I + + ++ ITMD EY+ KR+ N + + + G K L G+ GG+ + T+ +G + G +G G KG+VGVV +P GG++D+A+ + + E + + ++ +PPR
Sbjct: 2 GAAGMVSRITGTMGKGLATITMDEEYQRKRREEM-NQQPTDLKEGLARGG-KGLLKGVIGGVTGIITKPIEGARSGGATGFLKGVGKGLVGVVARPAGGVIDMASSTMEGIKRWQAEASETVEKMRPPR 128
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Yeast
Match: VPS13 (Protein involved in prospore membrane morphogenesis; peripheral membrane protein that localizes to the prospore membrane and at numerous membrane contact sites; involved in sporulation, vacuolar protein sorting, prospore membrane formation during sporulation, and protein-Golgi retention; required for mitochondrial integrity; contains a PH-like domain; homologous to human CHAC and COH1 which are involved in Chorea-acanthocytosis and Cohen syndrome, respectively [Source:SGD;Acc:S000003963]) HSP 1 Score: 144.05 bits (362), Expect = 3.502e-34 Identity = 182/764 (23.82%), Postives = 322/764 (42.15%), Query Frame = 2
Query: 65 GKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLS------------------YVASLGYRILRNLQIEVRNVHVRYEDN----STLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIM-----SEVSVEN----IKSVLQEKLNEMPKSDCIVNPISLHGIMKRNINPL-PMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVF----AITE-HTRH-IFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRN--------NLESDLELDCLLQLREVAMEHIGR-------------KSRDLLQNAVNIPAPQQSGWWGYLW--GRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGG--------------EIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTE------NIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTH--EPSCVLLDLGRLQV 2107
G YVENF + G++ GD++L+NL L+ L+ L PI VKSG++G + L +P++ +++ I+IED Y++ P S E +E M+K+ +R K+R+ + + LS ++ SL +I+ NLQ+ ++N+H+RYED +T PS + GL ++ S S+D W + + + ++ +K + L+ + +Y +T + + S+EN K ++ K + PK I+ P+S G+ K +IN L QP I L + ++ + L +Y + + + K K RP +V E+P EW+ + I E H ++ ++ +++E+ Y + + + L K NLEA R+ L DL D ++ R VA + S ++ N A + +G W W G+ ++E +++ E Q L + D N + + Q E V+ + +G F++ K + + + +N V F RP S+ L + D +P N +++++S R SK + +FE NPL N L I +T+ + I + ++F S + NI AAE + +T I+ +LE + VSL + AP IILP L H + C ++D G + +
Sbjct: 15 GSYVENFDPNQLNVGIWSGDVKLKNLKLRKDCLDSL-NLPIDVKSGILGDLVLTVPWSSLKNKPVKIIIEDCYLLCSPRS-----EDHENDEEMIKR--------AFRLKMRKVSEWELTNQARILSTQSENKTSSSSSEKNNAGFMQSLTTKIIDNLQVTIKNIHLRYEDMDGIFTTGPSSV---GLTLNELSAVSTDSNW-APSFIDITQNITHKLLTLNSLCLYWNTDSPPLISDDDQDRSLENFVRGFKDMIASKNSTAPKHQYILKPVS--GLGKLSINKLGSTEEQPHIDLQMFYDEFGLELDDTEYNDILHVLSSIQLRQITKKFKKARPSFAVSENPTEWFKYIAACVINEIHEKNKMWTWESMKEKCEQRRLYTKLWVEKL--KLKNLEAPLRDPIQEAQLSELHKDLTYDEIILFRSVAKRQYAQYKLGMTEDSPTPTASSNIEPQTSNKSATKNNGSWLSSWWNGKPTEEVDEDLI----MTEEQRQELYDAIEFDENEDKGPVLQVPRE---RVELRVTSLLK------KGSFTIRK------KKQNLNLGSIIFENCKVDFAQRP--DSFLSSFQLNKFSLEDGSP---NALYKHIISVRNSSKDQSSIDNHATGEEEEEDEPLLRASFELNPLDGLADSN----LNIKLLGMTVFYHVHFITEVHKFFKASNQHMETIGNIVNAAEATVEGWTTQTRMGIESLLEDHKT---VNVSLDLQAPLIILP---LDPHDWDTPCAIIDAGHMSI 722 HSP 2 Score: 90.8929 bits (224), Expect = 4.196e-18 Identity = 104/455 (22.86%), Postives = 179/455 (39.34%), Query Frame = 2
Query: 10106 NIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY--EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFS-----SLQHQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKK-----------LFP-SLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSD-----------QDV--------YNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKR---QRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPY 11347
N ++ S +G G+ LIN +ELLY +++ + + +Q+DNQ Y+ ++Y E +E + S + V F + + I I L+E ML M P + D+ L S IY + V+ LS I ++ + L + + +F +L + A + ++ ++V ++ I HY Q Q KI GS D GNP+G + I++G+ L+ + Q++ + K G+ DS +K S ++ +S +T D E++ R QR+ +N R + +N+AQ S A + G+ + K + EG +G G KGIVG+ TK G LDL + + + + R PR + + PY
Sbjct: 2582 NTQIVVSFKGVGISLINGRLQELLYINMRGIELRYNESKAYQTFSWKMKWMQIDNQLFSGNYSNILYPTEIPYTEKEIENHPVISGSISKVNDSLQAVPYFKHVTLLIQEFSIQLDEDMLYAMMDFIKFPGSPWIMDSRDYKYDEEIQLPDVSELKTAGDIYFEIFHIQPTVLHLSFIRSDEISPGLAEETEESFSSSLYYVHMFAMTLGNINEAPVKVNSLFMDNVRVPLPILMDHIERHYTTQFVYQIHKILGSADCFGNPVGLFNTISSGVWDLFYEPYQGYMMNDRPQEIGIHLAKGGLSFAKKTVFGLSDSMSKFTGSMAKGLS-VTQDLEFQRVRRLQQRINKNNRNALANSAQ------SFASTLGSGLSGIALDPYKAMQKEGAAGFLKGLGKGIVGLPTKTAIGFLDLTSNLSQGVKSTTTVLDMQKGCRVRLPRYVDHDQIIKPY 3029 HSP 3 Score: 74.3294 bits (181), Expect = 4.105e-13 Identity = 138/575 (24.00%), Postives = 227/575 (39.48%), Query Frame = 2
Query: 7883 VVLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSS-----IDCLFYSPIEL---SENIFSWCTVNAPGQKI---ETEVFCVSSKSSFNASQEKNKVYLICTIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSH----------------SVLIRLYDDQNRLLELEVK-VDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQ------NKPIMALPASFLTFHWPRDDLDQLLTVQKL--DSN-HQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQ-------------NVSESSCRKHS----VLPPKSCIPYALDEPTLPPIINLCIEGGTQS 9445
+V++ T I LL + D P P+ + P ++ + S I + SE+I+ W + + + +FC S + S + I PLA K+ P I +SA ++EN LP D+ F++ + R + L T + + LDSF + +A S S+ + L QN LL+L+ K +D S + I S Y I+N T L Y QS + + ENE+ + +P F+F +K D + R R+ K + WS K+S + +G Q ASV N+G+ I G+GK++ +K++ + RY++ N P+ + + Q KP+ + R+ +D+ L ++ L DSN QP + G YL+ + N+ L++ I L A + D D P+ + N ++ FYQ +ESS R +P KS +PYA D PT L +E GT++
Sbjct: 1987 LVIENTTSTEIELLVDSKD----PNKPSLKYAIKPHQSKSVPVEYAYDSDIRIRPASEDIYDWSQQTLSWKSLLSNQMSIFCSSKEDSNQRFH-----FEIGAKYDEREPLA-KIFPHMKIVVSASMTIENLLPADINFSIFDK---------------REEKRTDFLKTGESMEVHH-ISLDSFLLMSVQPLQDEASASKPSIVNTPHKSPLNPEDSLSLTLSGGQNLLLKLDYKNIDGTRSKVIR--IYSPYIIMNSTDREL-YIQSSLLNIAQSKILLENEKRYT-IPKMFSF-DKEDDKSNRARIRFK---------ESEWSSKLSFDAIG----QSFDASVRIKNKEQESNLGINISEGKGKYLLSKVIEIAPRYIISNTLDIPIEVCETGSMDVQQIESNITKPLYRM----------RNIVDKQLVLKFLGGDSNWSQPFFIKNVGV---------TYLKVLKNSRHKLLKIEILLDKATIFIRIKDGGD-RWPFSIRNFSDHDFIFYQRDPRKVSDPYKDDQSNESSSRSFKPIFYRIPSKSIMPYAWDFPTAKEKY-LVLESGTRT 2496
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Nematostella
Match: EDO41450 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4C6]) HSP 1 Score: 315.849 bits (808), Expect = 1.250e-85 Identity = 235/760 (30.92%), Postives = 378/760 (49.74%), Query Frame = 2
Query: 9284 ENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINL-CIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL---------------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKL-GMKCYINASCKNA-----AVFGGFETFKEFYAPL---VYKNWFRPGSGEVLVSVVTDGPTRVMRLIDK---------------EDESILSQSQLDFNADSSTFSNIEMHFSVE---GFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY----EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELL---EEDFSSL---QHQSHFDEG---RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKK-LFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPR--CLSSSWKLLPYQTSYSNN 11368
++ T + V F+Q+ +E R ++L K +PY+ DEPTLPP ++L ++G + + Y +N +G G+ LYY N +YI ++F S N+ LV DV P V L +K + RSQLW + L HEGS DP++ ++ + L+LD+D LAP P+ + R++L+K + RR+ Q W FTE G + L G+ AV G KE PL + RPGSG + V V+ DGPTRV+R+ D E + L + + + D+ ++ E+ V G GV ++N++PEEL+Y ++V + R + L IG+IQVDNQ ++ ++Y E+ E ++ Q + +E +V ++ + + + M + +EE++LLK +Q F K+ +L EED L Q + G R+ Y L++ + +SV +A L DL +K L L + +A + ++ + R+H FE+ N S++ HY +LRSQ I GS+DFLGNP+G ++D+++G++ L +V + + AHG+ DS AKI S S + +MD +++E R+ + A GS+ AG + L G+ GG+ S+ TQ +G + G SG G KGIVG V KP G+LD A+G+A + R A S PP+ PR C + + Y +++
Sbjct: 4022 KHYTEVPVKFFQSGTEDRLR--TILQAKQSVPYSWDEPTLPPELSLGVVDGSSYARYFMNILGSGERLYYYNPIYIVFTHTFTSTDGNVCGSFHARSAAPDHGPPLDRRELVLDV-PQGTAVILRRKEPLKRSQLWRMTGTGLLRHEGSSPLSDPRKP--STSSSGLVLDIDSLAP-PTCGK--YVRLVLSKPNTRRKHSQTWAFTEDGRMTCGLPGLFVQSRGGISGLRDGVDAVLGPANITKEEQIPLEQCISTCKLRPGSGCMAVKVIADGPTRVLRITDSLLHGEEEEMRGWLLVEQKGALGTATVTRSLDTDPQAS-ELVIQVRLAGGIGVSVVNYVPEELVYGALEQIEVDYVRTPKIETLEANIGRIQVDNQLFNSTLPVLLYPTAAEKGSREEHATQQPTLHISASKEPTRIQNVEIYKGVEVTLRRMTLHVEERLLLKLLQFFGYVQKDDDLATTDEEDDECLYDNQRAAVTSSGPASRRYYFERLQINPMQLKVSVASAGRLSEDLASLKSSLGLILFNLEDATLDLESFIRDHPFESLNSHLDSMSKHYTEELRSQTAAIVGSIDFLGNPMGLLNDVSSGLEGLVKRGNVGGLFLNVAHGVSDSAAKITGSLSDGLWNASMDTKFKESRETM--RAERYGSSRDHFMAGMRGLGMGLVGGLTSIVTQPIEGAHNAGLSGFMTGLAKGIVGTVAKPTAGVLDFASGTAAAVRGQATRSSRYFPPKCTRPRRNCFGPNGSIPRYSRTHAEG 4770 HSP 2 Score: 287.345 bits (734), Expect = 5.417e-77 Identity = 251/936 (26.82%), Postives = 437/936 (46.69%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGP--NSNWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNE----MPKSDCIVNPISLHGIMKRNINPLPM-NHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFA-------ITEHTRHIFKGAALRERARNLVKYVQSYEDYLL-NKDSNLEAEFRNNLESDLELDCLLQLREVAM-------EHIGRKSRDLLQNAVNIPAPQQS-GWWG--YLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVN--FFTRDKIFLRGEFSLEGIKLKLLKDT-----EIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGE-----------------------------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAA-AEKHIRNA--------KARTSESIKKILE-----------KGS--------SDIRWW-VSLKISAPKIILP-NFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVGNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCL 2557
M+ L ++L +G+YVEN +T+ + G+ G + L NLPLK T L+ L + P+ VKSG IG + L IP R +S+ W I I+ +Y++AGP ++N ++E + E + KK LD +E +W++ ++ G W S +S I+ NLQ+ + +VH+RYED S FA G+ ID + +S++ +WE++ + + + K +DL +IY D+ ++ ++ ++ LQ +++ + K I+ IS ++RN LP+ + PR + + +E++ +SL++ QY++ + L EFERF RQ + K+RP+V V + K WW FA I E RH A + RA +LV YV+ Y ++L+ N+ + + E +E +L + L LR +AM E + + D+ A + P S GW G Y W + +P + N++ +E P + E E L+E+ + N F RDK+F R L+ KLL T PI+EV N E RPQ S+W + L ++ RD K F +VS +E + + +F + FE P S + +I+++PL IV P +I + ++FS T + + E+ +R A K +T +K+ L+ +GS +++ W + L++SAP+ +P NF K + S VL+D G L N + ++R +++ ++D+E + FVTP S+P E+ + A C + N+ E K++ M W S+++ + + L +++ F L L+++ + Q P +++ N+ L +++ K C
Sbjct: 1 MLEGLAAWILRTYVGEYVENLNTEQLSIGV--GTVDLHNLPLKKTALQGL-DLPLEVKSGFIGHLQLSIPLRRPKSEPWIINIDKLYLVAGPPLHNNEYNEQQEAEREWIRKKRQLDLLESQWQESQQKGQTGGFWSGSWWPSLYSSFSTTIVENLQLVISDVHIRYEDASIDQHTPFACGITIDRLAAQSTNDKWETEEVIQNDHSIKYKLIDLQNFAIYWDSDAPLVGDLPSTKLEVALQRTIDKSSGSIGKHSYILESISASAKLQRNATALPLRDKNPRFVVDVHVERIPLSLAEVQYRMGMKLMGEFERFFRQARYHKWRPKVPVKNNAKLWWQFASTCILHFIQERNRHHTLSFATK-RAHDLVVYVKLYTEHLMSNQLESAKKEELERIEQELSFEELAVLRRLAMRKAEKALELVKASTPDVAPVAPSEPTSTASQGWLGGWYSWYWSADGAAPSKTGDATDNTKPPFKIE---PPSTKEEE--------EFLDELSDPSRNQSIFNRDKVFARLNICLDSGSFKLLNSTSSGHSSTPIVEVEFCNATWESEVRPQSSTWEFNMSLGAIHARDRF--TKGTLFASLVSPQERKPLAAQKSSNHSSISMLAMSSTALGLNILQEFDEEPVFEMRFEQMPPKS----KVAYRFSITTQPLDIVWNPSVIDRVSDFFSNDTLSAQYVNVERQLREAAWLRYEELKNQTKAELKQTLDSMMEGVQIVSTRGSITDGALNKGNVKSWDIQLEVSAPRFFVPENF--KDRKSSVVLVDFGHLSFKN--SEAMEREKRISE----EEDTDVEDDSFVTPMSTPPSEQEDENDAGICSTESAMRLAENLNPE---KIKEAMYDKDKW----SDVTSHHFGSSDLHVVDRFTIKLTLERRLIFTADPQCPAVLLSGNLPSLTFHVNEYKCHALTKCF 900 HSP 3 Score: 209.149 bits (531), Expect = 2.288e-53 Identity = 164/529 (31.00%), Postives = 260/529 (49.15%), Query Frame = 2
Query: 4586 NKLASIKIRLIDKKFHGFST---ECDSISRFVDADFNQLTFRISMEAWVILLDFFGLG--------SRFYDPKDVKDL--LDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENN----------------------DRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLM-----SRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMR 6052
K S+ + +D K+ S E S+ R V DFN L I+++ WV++L+FFG+G S P+ + +D+ +P + D + INTK++I + +L + + + + + G+S D+ +GNM G L++ L D+SP G++Y ++ + L F FKYG PD L R DM L ++S + Y+HT+RF EL F Q++LQ Q+ L IR AG V R +R+ + I +P++L+P SA S HML+ DLG +L N F +E + R CLLD M IDL +MD+++A+R + + +F+Y P K L EKC LNL V RNL+ S PD+ G LSSV + QY I G+L N GE + + + ++ DP+ + + +++ + L +V L L+ + + +L R++F +S +E+FSD SKTI+LV ++ ED R
Sbjct: 1978 TKYKSVSHKELDTKYKSVSHKEFETKSVHRRVQVDFNSLDTVINLQTWVLVLEFFGIGVAPPPSQASSPVTPQAPANFGSMDLDDQMP-LDTSPDN--NKSINTKVDIQVASLTLTVNKSQYPLAKAKVTGLSCY-LDM----SEGNMAAEGKLTNISLTDMSPHGALYREKFKTCGKE--ALSFQFFKYGEPDLDLDRPFDMSLVLRMS-SVQYVHTKRFQSELVAFCQHYLQLQEVLGRIRAEDAGNAVSWMIGRGSRVLLDIQAGSPVILVPRSAKSSHMLVADLGDLTLKNCFLWEESPGTISHNTGQKRRTLSDASKAKTRHTCLLDCMTIDLVDMDLFTAVREPEVPVSR---EFTYT---PQGVKLLKEKCRLNLQVERNLDWAFSRAVPDFLFSGRLSSVSAGLDYSQYCLILGLLGENFGEELEEFE--RPSSYLHDPLGPPPESEDVWTTLRMSIVLVNVSLELLPVQLFEHPEQASDAPLPSLARIDFIRSTFEFETFSDWSKTIDLVSSEVIFEDTR 2487 HSP 4 Score: 100.138 bits (248), Expect = 1.861e-20 Identity = 139/620 (22.42%), Postives = 247/620 (39.84%), Query Frame = 2
Query: 6413 IQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAV-SAFKIRLSFSDIQLLGKIIRSFQ---NQTIPPANLAYQT-------GYLRHRWSSLNFDNVN-------TSKPSQPNLTALQKLSKFVDRISIHHLDDYEF---------VSVCLIDDCIDADVPLFDIKFNNVTVDIEL-GNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICW-FWLTTKK------FSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQER-----------------------NVAFCDCRKRHNNL-----------------------------------FVPFLIVNELGCPIEYRKLS-------LDNPPMYNEIWNANKCGDE--NKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY--IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRP 7954
+Q+ F EL +N+ + F +LE+ T+A++L + +++ S S+ + SC+ S + SII+P +I + G+ ++ +L V+I + S+F +R+S++D +L I S N+ + + A Q L R L F+ V+ TS + + T L + + + + ++F V VCLIDDC D DVPL ++ + +L G L+V+YYN GW P IEPW + W TKK L + L+ +++ L+ + T W+ + R N R R ++ FVP+ I N GC + +R ++ + N + +A+ G + ++W V A E ++R+K+R+ +H H+I +++EGW+ PV+VD++G F+R Q DG + S RIV ++ +G + + ++S L + N +IP+E S + ++P+ + IPL T + RP
Sbjct: 2650 VQKTF---ELKVNISGTEFVVLESLGTRDTNAVVLKLTAVLAWRPHLQTQKWLSCSIQNLEMFSCIFSCEEDTAQSIIDPTTALIDLNNARRQL-GRRTVG-LLEITSEPLPSVEITLQSSFNMRISYNDYKLYLSIFDSVMRQLNKAMKTESKAKQEIPDHDFDDALVRRLMELGFEEVDCINALIATSGRLEASATWLLENATPIIPAKQGDGEGWQFAGFEVRAGTVCVCLIDDCGDTDVPLIELSTQGLYFWHDLQATGDGAANCTLTVEYYNRMVSGWEPWIEPWKCQLHWQQQQKTKKTPKRLLVKLDAPDRLDITVTTVLINMINQTLSSWTEDLYGRADSLSSPAPSAATSSTTLVAAGSNSTLVGSRSRDDSFSRSVSVESGGGSPERRPESSTSLVSLGGQHRRRAPFVPYAIRNNTGCLLMFRTITTTPSRVIITNTGLKLAPHDAHGPGYDKMSEWREV----AAGEEIPFEFSSREKMRHKD--THDFIVHQIVVQVEGWAPVTPVSVDRVGKFFRQAQPQATDGNPVASDIHPARIVFDVTLEGGARKVVSVQSSLSVLNKTDIPVEVKLQGPTSYQGHVTLPPIQPHKHMCIPLRAVTWQLYARP 3258 HSP 5 Score: 97.4413 bits (241), Expect = 1.548e-19 Identity = 76/313 (24.28%), Postives = 131/313 (41.85%), Query Frame = 2
Query: 8198 PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVL--IRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGT-TTLQLRAASVYQNRPNLL----------------------------------------YNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNK 9007
P H I+I AP L N LPCD+ + +KS + + ++ + + + +++F C+ L + L+ + + L D R L L V V RP G + + I + YW+VN T +PL++ Q G + + AG E+E A S P F+F +++ ++R+G+ KP+W SL+ + R A ++ NL+ Y+IG+++R GRG+ T++V RY L+N++ L F Q + DQ +
Sbjct: 3455 PAHTISIVAPVVLINLLPCDLSYQVKS---------VGVKGDIKAGRSTPIYSADPDKPFDLGFSIENFTYCENLRVTKRGNLAEHPITHVELRDTNRRSLVLNVLVIVRPGGALRISIFAPYWMVNNTGIPLIFRQEGSSHEM-AGQFEEHEMARSLTPLLFSFIDRDTPGRCQMRIGNSYHAGTG---KPIWCSPFSLDTPREFNRVHARQADGRPDKSNLIDYPEDDEDEDTAKAPRRNPFDFGDTNTPALDFSFGADSKVYDIGIDVRFGRGRFCLTRIVTFATRYQLENKTPHTLAFSQRHFVRDQGE 3754 HSP 6 Score: 86.2705 bits (212), Expect = 3.701e-16 Identity = 63/228 (27.63%), Postives = 113/228 (49.56%), Query Frame = 2
Query: 10133 GFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMY----EENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELL---EEDFSSL---QHQSHFDEG---RKIYIGNLKLLTGVISLSVITAESLPSDLKQMK-KLFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHY 10774
G GV ++N++PEEL+Y ++V + R + L IG+IQVDNQ ++ ++Y E+ E ++ Q + +E +V ++ + + + M + +EE++LLK +Q F K+ +L EED L Q + G R+ Y L++ + +SV +A L DL +K L L + +A + ++ + R+H FE+ N S++ HY
Sbjct: 3797 GIGVSVVNYVPEELVYGALEQIEVDYVRTPKIETLEANIGRIQVDNQLFNSTLPVLLYPTAAEKGSREEHATQQPTLHISASKEPTRIQNVEIYKGVEVTLRRMTLHVEERLLLKLLQFFGYVQKDDDLATTDEEDDECLYDNQRAAVTSSGPASRRYYFERLQINPMQLKVSVASAGRLSEDLASLKSSLGLILFNLEDATLDLESFIRDHPFESLNSHLDSMSKHY 4024
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Nematostella
Match: EDO34505 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP53]) HSP 1 Score: 181.03 bits (458), Expect = 6.657e-45 Identity = 207/889 (23.28%), Postives = 399/889 (44.88%), Query Frame = 2
Query: 47 LLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWR-KKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKS-------DCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFA----ITEHTRHIFKGAALRERARNLV---KYVQSYEDYLLNKDSNLEAEFRN--NLESDLE-LDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQS---------------------QYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDF---EAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIP----ILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRST----ENIKAAAEKHIRNAKARTSESIKKILEKGS-SDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVG----NWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELT 2545
L+++ G+Y+EN ++ G++ GD +LENL LK + L+DL + P+ V G +G++ L+IP+ S+ I+ +Y++ PN+ EKE + + KK L++IE+ + ++ +++ +DK + S+V L ++++NLQI + N+HVRYED T P F+ G+ ++ S +S+D W + T + +++K ++L+ +S+Y +T I + E ++ +E + K ++ PIS +K + P++ L L +E++ + LS+ Q+ + L + F+ R KY+P V +M+SP +WW +A + E + + + + R+ +Y Y+ LLN+ + E + +N NLE +L+ L+ + ++ E + + A SGW+G + ++ PE +S L KL+ T EQ E+ + +L + + E + F ++ + + L+ I ++L D I++ + K+L RP S+ IR+ + ++ + +V+S++ + +F AFETNPL C ++ ++S PL IV+ I + ++F + + AAA + K + S+ +E +DI +L + +P I++P S ++LDLG L++ + DQ +R I + +NLSM A E+K YD++ I+LE + L+ +WN + KL ++ P + L L +K + + I + ++ + + I+D+KL+L
Sbjct: 10 LVNKFAGQYLENLDPSQLRLGIWGGDAQLENLSLKESALDDL-DLPVKVLRGHLGKLVLKIPWKNLYSEPVVAQIDGLYLLVRPNTGIHYNAEKEEKAQQEKKKRQLEAIELARKLEEEKKKAVSDKESKEDKDSFVEKLAMQVVKNLQIYISNIHVRYEDTVTHPDAPFSLGITLENLSAQSTDENWIPSI-VGTSVKIVHKLMELNSLSMYWNTGDDIGKLPTKEEWPTIAKEGIARTMKGFSKPEGFKYVLPPISATSKVKLDTKAGSDLSNPKVYLSLVLEEIFLVLSKTQFHDLMMLLESFDYMARNVPFRKYKPVVQLMKSPSKWWHYAMDCVLEEDVKRRSRNWSWKHIKRHRTTCREYKMLYKKKLLNEKLSKE-QLKNIENLEENLDVLNITICRQQAEYEEAAALRKKKRKEAEKEEGGMFSGWFGSKKKAKKQKDEPESALESTPESKKPSSKSHKKSSSKSSSSNKLSKISFLRKLMDGKILTEEQKKEE-LAKLYAAIGYSENEVITSFPKEYVENKVIVHLKQIGVQLRDDCGSGKPQEIVKFSIKDLYADLAQRP--SAQAIRVTAKVDKMKMYGSPGEGGHLPLMVTSQQEKEKGHMALFHAAFETNPLDEKCDQRVE----VTSRPLKIVYDANTIDEVMKFFQPPQDVHLQELSAAAMSGLEALKTASKASLAYAVEHHKVTDI----NLDVMSPYILIPEKGCMQGSKSVLVLDLGHLKIKSDPDQ--ERVI----------------------------TTKNLSM-----AELESK----CYDKFDIRLEQLQVLLAKEGDDWNAARQQTQS------KLHIVNPISIDLKL-QKALNPDDVRLAQIKLAGSLHSIDVHITDKKLDLA 838 HSP 2 Score: 72.4034 bits (176), Expect = 5.284e-12 Identity = 59/216 (27.31%), Postives = 103/216 (47.69%), Query Frame = 2
Query: 10721 NHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ-----------------DVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYE-EKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFR---EIARSSKEGQPPRRY 11305
N V+ L +I HY Q Q + +D LGNP G I+ + G K L+ + V ++V H G + ++I + + + ++MD +Y+ E+RQ + + G A+ G K L G++ G+ + T+ +G K++G +G F G KG+VGVV +P GG++D A+ + + + A K+ +PPR +
Sbjct: 3249 NQVYNQQQLTG-AIIKHYTGQAIKQMYVLVLGLDVLGNPFGLITGLKDGAKDLFYEPYQGLIQGPGEFAEGLALGVRSLVGHTVGGAAGAVSRITGTIGKGFAALSMDEKYQQERRQAMGKKPVNVGEGLAR---GGKGLLEGVFEGVTGIVTKPVQGAKEQGAAGFFKGLGKGVVGVVARPAGGIVDFASSTFEGIKGSADTATEVKKLRPPRVF 3460 HSP 3 Score: 60.4622 bits (145), Expect = 2.008e-8 Identity = 44/169 (26.04%), Postives = 85/169 (50.30%), Query Frame = 2
Query: 5282 SAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFEN-----NDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGEN 5773
+A +I D +Q +ARI++ +S QAP +++PV++ S L+ DLG+ + N FK + + ++D M ++L S+++++ +++ +GD + + + E NL + ++R+L+ H P + G L +V TI V+ NLGE
Sbjct: 1184 AAEKIQDIQSQ-AARIKLTVSIQAPFIIIPVNSHSRDALVADLGQLVVSNSFKLHSPATKPDGSDAVIVDNMVVEL------SSVKLSRALIGEGD--------QIVASSVILEPLNLTIYLARSLSA-WYHKIPGIDVTGELCAVKMCTGELDLSTILAVIFQNLGEG 1336
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Nematostella
Match: EDO31907 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWL5]) HSP 1 Score: 128.642 bits (322), Expect = 1.508e-29 Identity = 114/465 (24.52%), Postives = 213/465 (45.81%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQI--------------------EVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVE-NIKSVLQEKLNEMPKS----DCIVNPIS--LHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALRERARNLVKYVQSYEDYLLNK-----DSNLEAEFRN--NLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSG-WWGYLWG 1309
M QL+ +L+ V+G+Y+ + + N++ + +G L +L L++ L D F+ P+ V G +G ++L IP+T S I IED+Y+IA P EK +E +++ L IE KK + D + S+V L ++++NL+I V+N+H+RYED T FA G + S +++D W++ + + K V L +++Y + N K +++ ++ K + I+ P+S H IM + + + M+ P++ + + +++ +++SQ Q+ + + D FE + + KY+P V V + +WW +A T + + + +Y ++Y+ K DS E+ R+ LE DL + +L ++ A + + R++L PA SG WW +L G
Sbjct: 1 MFEQLVVSVLNSVLGRYIRDLDSSNLELAVLQGQAVLRDLELRDDAL-DGFDLPVQVTHGFVGEISLTIPWTNLYSSPCDICIEDVYLIASPVKEQPYDAEKARASELAVQRKRLRQIEESLAKKATESTDG-IAHAENGDSFVEKLMAQVIKNLKISPVQFIEERTENTLAALTTVSVKNIHMRYEDQVTSCESPFAVGFTLQNLSAETTDDNWKTSV-VNAAAKTVFKLVSLDNLAVYWNPDQHAYDLYGNHWNWKGKMEDGISTKAKQPKDYEYILQPLSAECHVIMDQT-SKVEMD-VPKLLVEVIQQELSLAMSQKQFHNLMDVIDSFELMSVNQQYKKYQPTVKVHGNASKWWKYAYTAICEEGIRSWSWERIKAHRARY-RAYKSLYKRKLKGFHDSERESSLRHLQALEDDLGIPSVLIAKQQAELEVPPEMREVLS-----PAKTSSGSWWSWLGG 454
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Nematostella
Match: EDO31906 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWL4]) HSP 1 Score: 105.531 bits (262), Expect = 5.082e-23 Identity = 60/198 (30.30%), Postives = 105/198 (53.03%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESK 613
M QL+ +L+ V+G+Y+ + + N++ + +G L +L L++ L D F+ P+ V G +G ++L IP+T S I IED+Y+IA P EK +E +++ L IE KK + D + S+V L ++++NL++ V+N+H+RYED T FA G + S +++D W++
Sbjct: 1 MFEQLVVSVLNSVLGRYIRDLDSSNLELAVLQGQAVLRDLELRDDAL-DGFDLPVQVTHGFVGEISLTIPWTNLYSSPCDICIEDVYLIASPVKEQPYDAEKARASELAVQRKRLRQIEESLAKKATESTDG-IAHAENGDSFVEKLMAQVIKNLKVSVKNIHMRYEDQVTSCESPFAVGFTLQNLSAETTDDNWKTS 196
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Nematostella
Match: EDO42080 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2Q7]) HSP 1 Score: 93.2041 bits (230), Expect = 2.584e-21 Identity = 52/168 (30.95%), Postives = 91/168 (54.17%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKL-FEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYED 523
M QL+ +L+ V+G+Y+ + + N++ + +G L +L L++ L D F+ P+ V G +G ++L IP+T S I IED+Y+IA P EK +E +++ L IE KK + + S+V L ++++NL++ V+N+H+RYED
Sbjct: 1 MFEQLVVSVLNSVLGRYIRDLDSSNLELAVLQGQAVLRDLELRDDAL-DGFDLPVQVTHGFVGEISLTIPWTNLYSSPCDICIEDVYLIASPVKEQPYDAEKARASELAVQRKRLRQIEESLAKKATES----TAHAENGDSFVEKLMAQVVKNLKVSVKNIHIRYED 163
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Medaka
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:NCBI gene;Acc:101163959]) HSP 1 Score: 1082.01 bits (2797), Expect = 0.000e+0 Identity = 1143/4358 (26.23%), Postives = 1916/4358 (43.97%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS-NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVL-QEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR-------ERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAP------QQSG----------W---WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLE-GIKLKLLKDTEIPIL--------EVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGE---------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLE-NLSMATDCQASKETKPVTNI-----------YDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFM---NNYRNADSSSVKYKKQTRMDNLNKSP-EINQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSES--------------------------IG------------DPIIEFTYKISQKSGSS---DIQRQINLI-FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKP---------ETPNTTEFKEFDVSIACKAVSILLIKISSN---LASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDIS----------ILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEESTRIGFLN--------------------------------------SVKLHL--RMLSPILALPRHKTSNQIILVNLGDIEITS-------DTQSLQVAFSDA---------------------------IVISKELPIENG--------GYDIGICTDNNAKF-----PLVNKIDLMLKFSK--LHDPPKILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDE---------------------IPKPFIEFEDENMQEDQNPDP-SENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIP----PPSPFYKYSKRKKSLSNS----VDIQNCAAFELKLPKNTVKSTEP----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEII---NTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKI-----------SCQAPLLL-------------LPVSA------------FSDHMLI---------VDLGKFSLVNEFKFENNDR--------------------SRCLLDEMKIDLHEMDVYSAIRITDSILKDG------DLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANK----RLNVFPIIISPDK-NTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP-----------------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKP------------SQPNLTALQKLS---KFVDRISIHHLDDYEFVSVCLID----------------------------------------DCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF---------------------------QERNVAFCDCRKRHN--NL-----------------FVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTY--IRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP---LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETF---KEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSV---EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEE--KMLLKTMQMFL------------------SPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLL 11341
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L + F+ P VK+G IG++TL+IP+ R S W I + + +I GP EKE ++EN KK LL ++E K++ + Q+G++ W S ASL RI+ N+++++++VH+R+ED+S+ F+ G+ I+ S ++ E K + KQ+++ S+Y DT+C+++ ++ I+ + Q LN + I P+ +++RN + PL PRI +Q+E + + LSQ QY + E +R +R + K+RP+V + + ++WW+FAI E ++ + R +RAR+ Y Y L + E + +E + + L LRE+ + R+ D + +V Q+SG W WG +G PE + LP S+ S G +D+ + L D +N FTR D +F R EF LE G + +D P+L E + +IV ES P+ S + + L +LF+RDL + F ++S++ V G E +F + +E NP S +R L + + PL I++ P I + E+F + + + AA + K +T I++ +++ + RW + L I AP++I P+ ++ +P V++DLGR+ ++N D+ +I + EP D+ +++ TP ++P S L + Q P I Y++Y + ++ +VG NW L E E+ ++E FN +L L+++ Q P ++ + L + + +K+ + CL +++ + Y D SS+K + + NL +S + Q+ + V + F + + +ES R ++ L + T+ + +S+ + L+L D GS D ++ASHK L D P L ES +G D +I+ Y+ S D Q+ I ++LD+I N ET+ ELL F++ F + Q+P T T + ++++ +++LL++ S L +E + ++ I+ +++N+ M G+ + + + + + D++ + S+ N L L G D +N ++ E + G + + + Y HS +FL + +E + F + N AA + +L+S+ + G ++ SVKL+L + SP++++PR ++++ +LG I I + ++LQV D ++ +E P G I D +F PL + L ++ ++ I+ EF + + + K VYEQVL+++DNL +E P P + Q P P S + + + ++++++++L D S+ ++ + Q + +F + + ++LA+ + + +L+ + K L+ S + + +E S SCP Q + Y ++ +L + F Y + + SNS C P + S +P + L I + L+D+ F T S+ R VD DFN L I+++ WV++LDFFG+GS + VK+ + + +P F YD +++E + NTK+++ + SL+L + +N A VS + L+ DG+M L GSL L DL+P GS+Y +R F D ++ F++ KYG PDP L RE D+ + +++ + Y+HTQRF EL F +F Q QD L R G+ V QR++R+ + I S Q+P ++ LP A + H+++ + L S+ +E K N + CLLD M +DL EMD+++A R + G D+ F +R L + C L L V RNL+ +SH PD I+G LSSV S+ + Y+ IRG+L +NLGE + L+ Y Q DP +T L+G Y + + + DV L L+ ++ + +F +SKL +ESFS+ SK++ LV + D RYS P + R NVF I+ P K T R+ + QL + Y S + + F V LN ++ +IFDW+Q+ +++ L DE R SK + R SF V+ ++ + Q+ E+ +NV + F ++E+ S L ++A+IL +YK R +R PFS S++ + SC + S+ LSII+PV + + +C G P+ L D + ++ L ++I A IRLS++DIQL I +SF P A+ S + D V+ S P S+P L+ L L + R IH + + L++ DC+D D+PL ++ F+ V +G+++ +F LS DYYN GW P IEPWP ++ W K + S+ L+ +I+S LL+ K T W ++ Q +A R N NL FVP+ + N GC + + L+ + ++ + V DS S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+GIF+R+ D + S +R+V I +GS + I +RS L + N L++ +E + V+ + P + IPL + +Q RP LF+ + + W +V P + ++ C S + ++ S ++N + + I++ N+P A + V PGH I + L N+LPC++ + +K + + + P + + Q+ V L+SFP C LLI + ++ V +RLYD + RLL L +++ R G + ++IS+ YW++N+T LPL++ Q +G+ + AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRS+ L F Q Q I LP S + FHWPR+D DQLL V+ ++ + SGGF+++ SFH+ +R N LR ITL+GA Y++ FT+AD LP P +++NL+ + + F+Q+ + R H+ + P S + YA DEPTLPP + + ++G S D+N LYY+NF+YIA ++F + LV DV ++V L +K RSQLW + L HEGS P + K R D+ L+LD+ LA + S ++L + D RR Q W+F+ G + + + A ++ G E AP + F RPGSG + V V+ DGPTRV++ + +L ++ + N F +E +V EG G+ L+N PEEL++A VD+ + R + L + + IQVDNQ + + ++ +P+SS + E G V+ P ++L E K L+ T Q F E E+L+E SL + + G ++ Y NLK+ I LSV T++ LP DLK +K FP L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D+T G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ S+ T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A SS R +PRC LL
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRE-FDLPYEVKAGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPAPPQEYDEEKEREDENERKKHLLKALEDKFKCECEQKGES------YWYSVTASLVTRIVENIELKIQDVHLRFEDDSSNLENPFSFGVCINNVSAQNPAKEMVQKL-------LRQKQLEIQDFSVYWDTECEMLGKLPANQIQDAMTQCMLNR--EHQYIFEPVCASALVRRNASKEPLRSRSTPRIEGLVQLEPMSLRLSQVQYLQIMTFLKELDRRERGMFYRKWRPKVPIHTNCRQWWMFAI-EANLNVIREQRRRCSWIFSLQRARDARTYTSLYFTRLRGIPLSPLEELKR-VEEEQTFEELHTLREIVYDWF-RQQEDSTEPSVECQPELSATTIQKSGSSGMIQYLQSWFPGWGGWYGDSENLVRPE----------------EPLPSPSSWSILGQTEDLFDPLE--DSHTLNTFTRRDHMFARLEFLLEKGTIMLFEQDRRGPVLHESGVIHLEFSVVKVIV--ESLPRSESSLLSVKLGNLFLRDLT--TQGSIFPVLMSTK---TVSGSERELVESTSPVFEMIYERNPAKS----KFERRLEVRTSPLNIIYNPQAIKKVTEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKRWTMKLDICAPQVIFPD-DFQSGDPVLVVVDLGRILLTNSQDE---STISSKSAEP--EREDMSDDEYQTPLATPPESPPPELDLPLKAQLKNPEVPSLKIPESTQAYSWKLYEKYCLSFMDLQIMVGRYKDNWKHLQEGEVGPTH-------VVEKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHFNLEKMTALQSCLARLSSPAPSEHKYFFRDDSSLKLQGSAK--NLTQSVMTLEQHTREVLVESRLLLAEFNVAYMQLGVESEGRYISVLKVFGTKAHFVKRPYDAEVSLTVHGLLLVDTMQTYGSDFDLLVASHKHLGFDLPTGILKESQPSSPDLCNNTSEHEERLPEFSSNLSMGNTSPFSSIFKDEDALIKLEYQFVSSDCPSMNLDSSLQVTSIQVNNLDIILNPETIVELLKFLQKSFPREDSSSVQQQPTHLHNEDEDRTYQATYDQNKELTVEIHRLNLLLLRTVSTGTTLGAEKKAMKIATASIAGTKVNVSM---GSTFDVSGSLGLMQLGDLTQEGNRSQFVVSIGSVEGNSLGLD-GLTFLSDTCGSQTDALNFRLL-EQSRGECSLKLHMASLHYNHSAKFLRELSLSANELEDSFRSMLKN-AATKVSTVLASKTAEYSGMVSLFETPLKKSRTFSHSWPYSFEEEEEVPVEEPESTLDDFSVKLNLNIHIESPVVSIPRKFGHPELLVGHLGSIRICNCLNSQDLPGETLQVLVKDIRLYSLNMSHLIPKRAPRWERVDDVLRYLINQEEAPFTRNDFFESLSRGQAFHILKDTTIQFTVEKLPLDEDAEQSLSTTEESYRSVGQLRIQGEFVTPVQVFLCKPVYEQVLQTLDNLFLIEEQHTMPSQPPTPPPPTPSSVRLHWFPDPQGGLFARDPPSSQKPAPYSASFTQLKVNLHVAELQVQLSADLTQGSQG-LVSVRFQDLEGDFTKDHPNSLRVQLALRAVLMEDLLEQNPESKYKYLMVSRGAPKPSTFSPKEYLSQSCPS---QSNAFYPDMPRSLPAHMEEAQNVFQLYQRHSSTPSNSNRKSKKDPECPITPPPSPLHCKASLQPSDFDDSLVHINVLLVDQNHPEFKTRYGSVGRSVDIDFNCLDVLITLQTWVVILDFFGIGSTASN-HAVKEEIP-PQAVPDFPL-YDPDVDETVQPVNTKVDLKVHSLSLVLNKK---LNELARASVSKLSAHLEML--DGDMALHGSLRSLSLSDLTPHGSLYGER-FTTQGGDALV-FNILKYGQPDPHLERECDIMVSLQMA-SVQYVHTQRFQAELVAFIHHFTQLQDILGRQRAAMEGQTVRDCPQRASRVLLDIDAGAPVIVIPESSQSPRVIVANFGQLRVKNRFLPAGACGTFSLREKVEKLTMHLMMCCQRHKYLPLPLWHISISSELKLTLNSCQTLLVLKSLQNKPHLMMFFCAACLLDCMALDLQEMDIFAAERFPSCLWDIGNNTESSDIVFPSYFVRRTGDNLLKDCCRLKLQVERNLDRELSHAVPDMSIHGSLSSVHCSLDRKHYQLIRGLLENNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVYTNLSFLMNMMDVSLELL------ESSGQTQHRQFDFVKSKLLFESFSNGSKSVNLVSHSLLANDTRYSE-PNRRTDGSRPNVFDCILQPSKTGTNRA-----SLQLELHYRSTRESSCFTVLLNNLRVFLIFDWLQLVRDF----------LQMPDEKRSS-------GSKSRQRGSSFSSTDSGALGTVMPKTVKSGVVTKRSTVPVTQDHC--LEVKINVTGTEFVVVEDRSSLESNAIILKGTTVLTYKPRLLDR--PFSGSLAGVEVFSCRLGSEQETALSIIDPVNVQV----DLC---GSPTYQSSSGLLDAFNVEDIPPL-LEIQFPALDIRLSYNDIQLFLAIAKSF-----PTASSIL----------SPDTDRVDASPPLSDGFSQKTNTISEPQLSHLLDLGFRREDCKRALIHCKGQLDLAATWLLENAEHVVGHSRSTADPGAGSHSAPLSGVEIKAENLCICFIDDCLDCDIPLAELTFSRFCVLQRIGSTQEGNASFTLSGDYYNRELSGWEPFIEPWPCSLSWQQQAAGRLHPPRFKMGVRSKQRLDVNITSVLLEQYKTTKSSWMADYCREEEHTPSSSSSPPWMGSSVDPPSFGQSAPLAHLRTRSAVNMVNLQQQVHYTGAVMSKRRQPFVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISDIHSSGVDDSHNVSQWREVLPGEELPFEFEAREKLRHRH--THELKLHQLLVRVCGWEQVKPVSVDKVGIFFRYAAPDRNSSSSTFSALPPVRVVFAITMEGSARKVITVRSSLMVKNRLDVLMEVRLDSPSAPDKPMVLPPILPGQALAIPLHLTSWRLQARPKGMG------------LFFCKVPI-----HWTSVERPEEISSSKRECQS--ADYDDSHKQNFRFCV-VIKKENYPDQLPAKRQVSGSTQEIYRQPGHTIYLLPTMVLVNQLPCELNYYIK---------GVHIQGSLNPGKEAVLHLADTSQNLELGVLLESFPVCKELLIPPGTQ-NYVVRMRLYDTEKRLLCLTIRIILRAQGALKILISAPYWLINKTGLPLIFRQDNGKAD--AAGQFEEHELARSLSPLLFCYTDKEQPAMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGISVRKGKGQYRDTHIVTFAPRYLLDNRSTHKLAFAQREFARGQGTSNPEGYISTLPGSSVVFHWPRNDFDQLLCVRLMEVTN---CTWSGGFEVNKPKSFHINMRDTLGN-CFFLRTEITLKGATYQISFTNADQLPPPLRIDNLSEVPIQFWQH-GVADLRLHTEVKPMSVLDYACDEPTLPPYLTITVKGAGSSDVTADMNFFKEFNKLYYENFIYIAATHTFSQTAETRPVGKKWLMSWSELVLDVDTKTQRVILRKKEPGKRSQLWRMTGTGMLCHEGSSPPQN-KPTQPRPLDSSLVLDIAGLA---AVSDNSFEPLMLRRPDSRRSTTQTWYFSSGMLTCGLPRLVVQVKGGI--AGLYDGAEVVLGPDSGLAPPGPEQQFINQKMRPGSGVLSVQVIPDGPTRVLQKRISNELLLLCRNSVLENDTGFCFFRLET-VTVNLEEGLGISLVNKSPEELVFATLFGVDIHFTRTVTNEVLELSVQNIQVDNQLLGSTQPVML-----CVTPNSSDCNLN-----ESGPALQVNSVK------VPSTLMLTELFKHLMVTAQRFTIIIEEKLLLKLLSFFGYGEAEAELEMLDE---SLPERPSEEAGPPKRYYFENLKISLPQIKLSVFTSQKLPPDLKALKGTLGFP-LIRFEDAVINMYPFTRVHPYETQEIIFNDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSERECIRYHGATSGEH---LVAGIHGLAHGIIGGMTSIITSTVEGVKMEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSSHRLGVQRVRKPRCCKGPQDLL 4136
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Medaka
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:NCBI gene;Acc:101163959]) HSP 1 Score: 1081.63 bits (2796), Expect = 0.000e+0 Identity = 1141/4353 (26.21%), Postives = 1915/4353 (43.99%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS-NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVL-QEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR-------ERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAP------QQSG----------W---WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLE-GIKLKLLKDTEIPIL--------EVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGE---------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLE-NLSMATDCQASKETKPVTNI-----------YDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFM---NNYRNADSSSVKYKKQTRMDNLNKSP-EINQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSES--------------------------IG------------DPIIEFTYKISQKSGSS---DIQRQINLI-FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKP---------ETPNTTEFKEFDVSIACKAVSILLIKISSN---LASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDIS----------ILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEESTRIGFLN--------------------------------------SVKLHL--RMLSPILALPRHKTSNQIILVNLGDIEITS-------DTQSLQVAFSDA---------------------------IVISKELPIENG--------GYDIGICTDNNAKF-----PLVNKIDLMLKFSK--LHDPPKILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDE---------------------IPKPFIEFEDENMQEDQNPDP-SENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIP----PPSPFYKYSKRKKSLSNS----VDIQNCAAFELKLPKNTVKSTEP----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEII---NTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKI-----------SCQAPLLL-------------LPVSA------------FSDHMLI---------VDLGKFSLVNEFKFENNDR--------------------SRCLLDEMKIDLHEMDVYSAIRITDSILKDG------DLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANK----RLNVFPIIISPDK-NTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP-----------------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKP------------SQPNLTALQKLS---KFVDRISIHHLDDYEFVSVCLID----------------------------------------DCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQE-------------------------RNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP---LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETF---KEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSV---EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEE--KMLLKTMQMFL------------------SPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLL 11341
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L + F+ P VK+G IG++TL+IP+ R S W I + + +I GP EKE ++EN KK LL ++E K++ + Q+G++ W S ASL RI+ N+++++++VH+R+ED+S+ F+ G+ I+ S ++ E K + KQ+++ S+Y DT+C+++ ++ I+ + Q LN + I P+ +++RN + PL PRI +Q+E + + LSQ QY + E +R +R + K+RP+V + + ++WW+FAI E ++ + R +RAR+ Y Y L + E + +E + + L LRE+ + R+ D + +V Q+SG W WG +G PE + LP S+ S G +D+ + L D +N FTR D +F R EF LE G + +D P+L E + +IV ES P+ S + + L +LF+RDL + F ++S++ V G E +F + +E NP S +R L + + PL I++ P I + E+F + + + AA + K +T I++ +++ + RW + L I AP++I P+ ++ +P V++DLGR+ ++N D+ +I + EP D+ +++ TP ++P S L + Q P I Y++Y + ++ +VG NW L E E+ ++E FN +L L+++ Q P ++ + L + + +K+ + CL +++ + Y D SS+K + + NL +S + Q+ + V + F + + +ES R ++ L + T+ + +S+ + L+L D GS D ++ASHK L D P L ES +G D +I+ Y+ S D Q+ I ++LD+I N ET+ ELL F++ F + Q+P T T + ++++ +++LL++ S L +E + ++ I+ +++N+ M G+ + + + + + D++ + S+ N L L G D +N ++ E + G + + + Y HS +FL + +E + F + N AA + +L+S+ + G ++ SVKL+L + SP++++PR ++++ +LG I I + ++LQV D ++ +E P G I D +F PL + L ++ ++ I+ EF + + + K VYEQVL+++DNL +E P P + Q P P S + + + ++++++++L D S+ ++ + Q + +F + + ++LA+ + + +L+ + K L+ S + + +E S SCP Q + Y ++ +L + F Y + + SNS C P + S +P + L I + L+D+ F T S+ R VD DFN L I+++ WV++LDFFG+GS + VK+ + + +P F YD +++E + NTK+++ + SL+L + +N A VS + L+ DG+M L GSL L DL+P GS+Y +R F D ++ F++ KYG PDP L RE D+ + +++ + Y+HTQRF EL F +F Q QD L R G+ V QR++R+ + I S Q+P ++ LP A + H+++ + L S+ +E K N + CLLD M +DL EMD+++A R + G D+ F +R L + C L L V RNL+ +SH PD I+G LSSV S+ + Y+ IRG+L +NLGE + L+ Y Q DP +T L+G Y + + + DV L L+ ++ + +F +SKL +ESFS+ SK++ LV + D RYS P + R NVF I+ P K T R+ + QL + Y S + + F V LN ++ +IFDW+Q+ +++ L DE R SK + R SF V+ ++ + Q+ E+ +NV + F ++E+ S L ++A+IL +YK R +R PFS S++ + SC + S+ LSII+PV + + +C G P+ L D + ++ L ++I A IRLS++DIQL I +SF P A+ S + D V+ S P S+P L+ L L + R IH + + L++ DC+D D+PL ++ F+ V +G+++ +F LS DYYN GW P IEPWP ++ W K + S+ L+ +I+S LL+ K T W ++ +NV R+ FVP+ + N GC + + L+ + ++ + V DS S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+GIF+R+ D + V + I R+V I +GS + I +RS L + N L++ +E + V+ + P + IPL + +Q RP LF+ + + W +V P + ++ C S + ++ S ++N + + I++ N+P A + V PGH I + L N+LPC++ + +K + + + P + + Q+ V L+SFP C LLI + ++ V +RLYD + RLL L +++ R G + ++IS+ YW++N+T LPL++ Q +G+ + AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRS+ L F Q Q I LP S + FHWPR+D DQLL V+ ++ + SGGF+++ SFH+ +R N LR ITL+GA Y++ FT+AD LP P +++NL+ + + F+Q+ + R H+ + P S + YA DEPTLPP + + ++G S D+N LYY+NF+YIA ++F + LV DV ++V L +K RSQLW + L HEGS P + K R D+ L+LD+ LA + S ++L + D RR Q W+F+ G + + + A ++ G E AP + F RPGSG + V V+ DGPTRV++ + +L ++ + N F +E +V EG G+ L+N PEEL++A VD+ + R + L + + IQVDNQ + + ++ +P+SS + E G V+ P ++L E K L+ T Q F E E+L+E SL + + G ++ Y NLK+ I LSV T++ LP DLK +K FP L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D+T G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ S+ T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A SS R +PRC LL
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRE-FDLPYEVKAGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPAPPQEYDEEKEREDENERKKHLLKALEDKFKCECEQKGES------YWYSVTASLVTRIVENIELKIQDVHLRFEDDSSNLENPFSFGVCINNVSAQNPAKEMVQKL-------LRQKQLEIQDFSVYWDTECEMLGKLPANQIQDAMTQCMLNR--EHQYIFEPVCASALVRRNASKEPLRSRSTPRIEGLVQLEPMSLRLSQVQYLQIMTFLKELDRRERGMFYRKWRPKVPIHTNCRQWWMFAI-EANLNVIREQRRRCSWIFSLQRARDARTYTSLYFTRLRGIPLSPLEELKR-VEEEQTFEELHTLREIVYDWF-RQQEDSTEPSVECQPELSATTIQKSGSSGMIQYLQSWFPGWGGWYGDSENLVRPE----------------EPLPSPSSWSILGQTEDLFDPLE--DSHTLNTFTRRDHMFARLEFLLEKGTIMLFEQDRRGPVLHESGVIHLEFSVVKVIV--ESLPRSESSLLSVKLGNLFLRDLT--TQGSIFPVLMSTK---TVSGSERELVESTSPVFEMIYERNPAKS----KFERRLEVRTSPLNIIYNPQAIKKVTEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKRWTMKLDICAPQVIFPD-DFQSGDPVLVVVDLGRILLTNSQDE---STISSKSAEP--EREDMSDDEYQTPLATPPESPPPELDLPLKAQLKNPEVPSLKIPESTQAYSWKLYEKYCLSFMDLQIMVGRYKDNWKHLQEGEVGPTH-------VVEKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHFNLEKMTALQSCLARLSSPAPSEHKYFFRDDSSLKLQGSAK--NLTQSVMTLEQHTREVLVESRLLLAEFNVAYMQLGVESEGRYISVLKVFGTKAHFVKRPYDAEVSLTVHGLLLVDTMQTYGSDFDLLVASHKHLGFDLPTGILKESQPSSPDLCNNTSEHEERLPEFSSNLSMGNTSPFSSIFKDEDALIKLEYQFVSSDCPSMNLDSSLQVTSIQVNNLDIILNPETIVELLKFLQKSFPREDSSSVQQQPTHLHNEDEDRTYQATYDQNKELTVEIHRLNLLLLRTVSTGTTLGAEKKAMKIATASIAGTKVNVSM---GSTFDVSGSLGLMQLGDLTQEGNRSQFVVSIGSVEGNSLGLD-GLTFLSDTCGSQTDALNFRLL-EQSRGECSLKLHMASLHYNHSAKFLRELSLSANELEDSFRSMLKN-AATKVSTVLASKTAEYSGMVSLFETPLKKSRTFSHSWPYSFEEEEEVPVEEPESTLDDFSVKLNLNIHIESPVVSIPRKFGHPELLVGHLGSIRICNCLNSQDLPGETLQVLVKDIRLYSLNMSHLIPKRAPRWERVDDVLRYLINQEEAPFTRNDFFESLSRGQAFHILKDTTIQFTVEKLPLDEDAEQSLSTTEESYRSVGQLRIQGEFVTPVQVFLCKPVYEQVLQTLDNLFLIEEQHTMPSQPPTPPPPTPSSVRLHWFPDPQGGLFARDPPSSQKPAPYSASFTQLKVNLHVAELQVQLSADLTQGSQG-LVSVRFQDLEGDFTKDHPNSLRVQLALRAVLMEDLLEQNPESKYKYLMVSRGAPKPSTFSPKEYLSQSCPS---QSNAFYPDMPRSLPAHMEEAQNVFQLYQRHSSTPSNSNRKSKKDPECPITPPPSPLHCKASLQPSDFDDSLVHINVLLVDQNHPEFKTRYGSVGRSVDIDFNCLDVLITLQTWVVILDFFGIGSTASN-HAVKEEIP-PQAVPDFPL-YDPDVDETVQPVNTKVDLKVHSLSLVLNKK---LNELARASVSKLSAHLEML--DGDMALHGSLRSLSLSDLTPHGSLYGER-FTTQGGDALV-FNILKYGQPDPHLERECDIMVSLQMA-SVQYVHTQRFQAELVAFIHHFTQLQDILGRQRAAMEGQTVRDCPQRASRVLLDIDAGAPVIVIPESSQSPRVIVANFGQLRVKNRFLPAGACGTFSLREKVEKLTMHLMMCCQRHKYLPLPLWHISISSELKLTLNSCQTLLVLKSLQNKPHLMMFFCAACLLDCMALDLQEMDIFAAERFPSCLWDIGNNTESSDIVFPSYFVRRTGDNLLKDCCRLKLQVERNLDRELSHAVPDMSIHGSLSSVHCSLDRKHYQLIRGLLENNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVYTNLSFLMNMMDVSLELL------ESSGQTQHRQFDFVKSKLLFESFSNGSKSVNLVSHSLLANDTRYSE-PNRRTDGSRPNVFDCILQPSKTGTNRA-----SLQLELHYRSTRESSCFTVLLNNLRVFLIFDWLQLVRDF----------LQMPDEKRSS-------GSKSRQRGSSFSSTDSGALGTVMPKTVKSGVVTKRSTVPVTQDHC--LEVKINVTGTEFVVVEDRSSLESNAIILKGTTVLTYKPRLLDR--PFSGSLAGVEVFSCRLGSEQETALSIIDPVNVQV----DLC---GSPTYQSSSGLLDAFNVEDIPPL-LEIQFPALDIRLSYNDIQLFLAIAKSF-----PTASSIL----------SPDTDRVDASPPLSDGFSQKTNTISEPQLSHLLDLGFRREDCKRALIHCKGQLDLAATWLLENAEHVVGHSRSTADPGAGSHSAPLSGVEIKAENLCICFIDDCLDCDIPLAELTFSRFCVLQRIGSTQEGNASFTLSGDYYNRELSGWEPFIEPWPCSLSWQQQAAGRLHPPRFKMGVRSKQRLDVNITSVLLEQYKTTKSSWMADYCREEEHTPSSSSSPPWMGSSVDPPSFGQNVRMSKRRQP----FVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISDIHSSGVDDSHNVSQWREVLPGEELPFEFEAREKLRHRH--THELKLHQLLVRVCGWEQVKPVSVDKVGIFFRYAAPDRNSSSSTVGSPISRTNIIHPHVYFSALPPVRVVFAITMEGSARKVITVRSSLMVKNRLDVLMEVRLDSPSAPDKPMVLPPILPGQALAIPLHLTSWRLQARPKGMG------------LFFCKVPI-----HWTSVERPEEISSSKRECQS--ADYDDSHKQNFRFCV-VIKKENYPDQLPAKRQVSGSTQEIYRQPGHTIYLLPTMVLVNQLPCELNYYIK---------GVHIQGSLNPGKEAVLHLADTSQNLELGVLLESFPVCKELLIPPGTQ-NYVVRMRLYDTEKRLLCLTIRIILRAQGALKILISAPYWLINKTGLPLIFRQDNGKAD--AAGQFEEHELARSLSPLLFCYTDKEQPAMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGISVRKGKGQYRDTHIVTFAPRYLLDNRSTHKLAFAQREFARGQGTSNPEGYISTLPGSSVVFHWPRNDFDQLLCVRLMEVTN---CTWSGGFEVNKPKSFHINMRDTLGN-CFFLRTEITLKGATYQISFTNADQLPPPLRIDNLSEVPIQFWQH-GVADLRLHTEVKPMSVLDYACDEPTLPPYLTITVKGAGSSDVTADMNFFKEFNKLYYENFIYIAATHTFSQTAETRPVGKKWLMSWSELVLDVDTKTQRVILRKKEPGKRSQLWRMTGTGMLCHEGSSPPQN-KPTQPRPLDSSLVLDIAGLA---AVSDNSFEPLMLRRPDSRRSTTQTWYFSSGMLTCGLPRLVVQVKGGI--AGLYDGAEVVLGPDSGLAPPGPEQQFINQKMRPGSGVLSVQVIPDGPTRVLQKRISNELLLLCRNSVLENDTGFCFFRLET-VTVNLEEGLGISLVNKSPEELVFATLFGVDIHFTRTVTNEVLELSVQNIQVDNQLLGSTQPVML-----CVTPNSSDCNLN-----ESGPALQVNSVK------VPSTLMLTELFKHLMVTAQRFTIIIEEKLLLKLLSFFGYGEAEAELEMLDE---SLPERPSEEAGPPKRYYFENLKISLPQIKLSVFTSQKLPPDLKALKGTLGFP-LIRFEDAVINMYPFTRVHPYETQEIIFNDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSERECIRYHGATSGEH---LVAGIHGLAHGIIGGMTSIITSTVEGVKMEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSSHRLGVQRVRKPRCCKGPQDLL 4127
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Medaka
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:NCBI gene;Acc:101163959]) HSP 1 Score: 1080.86 bits (2794), Expect = 0.000e+0 Identity = 1132/4323 (26.19%), Postives = 1909/4323 (44.16%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS-NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVL-QEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR-------ERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQ----------------NAVNIPAPQQSG-------W---WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLE-GIKLKLLKDTEIPIL--------EVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLN------PDYKNDS----FRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLE-NLSMATDCQASKETKPVTNI-----------YDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTN--------FM--------------NNYRNADSSSVKYKKQTRMDNLNKSP-EINQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSES--------------------------IG------------DPIIEFTYKISQKSGSS---DIQRQINLI-FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKP---------ETPNTTEFKEFDVSIACKAVSILLIKISSN---LASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDIS----------ILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEESTRIGFLN--------------------------------------SVKLHL--RMLSPILALPRHKTSNQIILVNLGDIEITS-------DTQSLQVAFSDAIVIS---------------------------KELPIENG--------GYDIGICTDNNAKF-----PLVNKIDLMLKFSK--LHDPPKILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDEI-----------------------PK--------PFIEFEDENMQED-----QNPDP-SENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIP----PPSPFYKYSKRKKSLSNS----VDIQNCAAFELKLPKNTVKSTEP----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEII---NTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEF-------KFENNDRSR-------------------------CLLDEMKIDLHEMDVYSAIRITDSILKDG------DLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANK----RLNVFPIIISPDK-NTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP-----------------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRE-NRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPA--------NLAYQTGYL--RHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQE-------------------------RNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP---LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETF---KEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNI-----EMHFSV---EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEE--KMLLKTMQMFL------------------SPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLL 11341
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L + F+ P VK+G IG++TL+IP+ R S W I + + +I GP EKE ++EN KK LL ++E K++ + Q+G++ W S ASL RI+ N+++++++VH+R+ED+S+ F+ G+ I+ S ++ E K + KQ+++ S+Y DT+C+++ ++ I+ + Q LN + I P+ +++RN + PL PRI +Q+E + + LSQ QY + E +R +R + K+RP+V + + ++WW+FAI E ++ + R +RAR+ Y Y L S LE +E + + L LRE+ + R+ D + +A I SG W WG +G PE + LP S+ S +D+ + L D +N FTR D +F R EF LE G + +D P+L E + +IV ES P+ S + + L +LF+RDL P + + +++ S +F + +E NP S +R L + + PL I++ P I + E+F + + + AA + K +T I++ +++ + RW + L I AP++I P+ ++ +P V++DLGR+ ++N D+ +I + EP D+ +++ TP ++P S L + Q P I Y++Y + ++ +VG NW L E E+ ++E FN +L L+++ Q P ++ + L + + +K+ + CL +++ FM + Y D SS+K + + NL +S + Q+ + V + F + + +ES R ++ L + T+ + +S+ + L+L D GS D ++ASHK L D P L ES +G D +I+ Y+ S D Q+ I ++LD+I N ET+ ELL F++ F + Q+P T T + ++++ +++LL++ S L +E + ++ I+ +++N+ M G+ + + + + + D++ + S+ N L L G D +N ++ E + G + + + Y HS +FL + +E + F + N AA + +L+S+ + G ++ SVKL+L + SP++++PR ++++ +LG I I + ++LQV D + S +E P G I D +F PL + L ++ ++ I+ EF + + + K VYEQVL+++DNL +E P+ P + F ++ Q P P S + + + ++++++++L D S+ ++ + Q + +F + + ++LA+ + + +L+ + K L+ S + + +E S SCP Q + Y ++ +L + F Y + + SNS C P + S +P + L I + L+D+ F T S+ R VD DFN L I+++ WV++LDFFG+GS + VK+ + + +P F YD +++E + NTK+++ + L + + + S + +Y DG+M L GSL L DL+P GS+Y +R F D ++ F++ KYG PDP L RE D+ + +++ + Y+HTQRF EL F +F Q QD L R G+ V QR++R+ + I AP++++P S+ S +++ + G+ + N F F ++ CLLD M +DL EMD+++A R + G D+ F +R L + C L L V RNL+ +SH PD I+G LSSV S+ + Y+ IRG+L +NLGE + L+ Y Q DP +T L+G Y + + + DV L L+ G + +L R +F +SKL +ESFS+ SK++ LV + D RYS P + R NVF I+ P K T R+ + QL + Y S + + F V LN ++ +IFDW+Q+ +++ L DE R SK + R SF V+ ++ + Q+ E+ +NV + F ++E+ S L ++A+IL +YK R +RP FS S++ + SC + S+ LSII+PV + + +C G P+ L D + ++ L +++ + F R SF + L ++ R QN + L + G L W N ++V S + A S + + I E + +C IDDC+D D+PL ++ F+ V +G+++ +F LS DYYN GW P IEPWP ++ W K + S+ L+ +I+S LL+ K T W ++ +NV R+ FVP+ + N GC + + L+ + ++ + V DS S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+GIF+R+ D + V + I R+V I +GS + I +RS L + N L++ +E + V+ + P + IPL + +Q RP LF+ + + W +V P + ++ C S+ ++ S ++N + + I++ N+P A + V PGH I + L N+LPC++ + +K + + + P + + Q+ V L+SFP C LLI + ++ V +RLYD + RLL L +++ R G + ++IS+ YW++N+T LPL++ Q +G+ + AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRS+ L F Q Q I LP S + FHWPR+D DQLL V+ ++ + SGGF+++ SFH+ +R N LR ITL+GA Y++ FT+AD LP P +++NL+ + + F+Q+ + R H+ + P S + YA DEPTLPP + + ++G S D+N LYY+NF+YIA ++F + LV DV ++V L +K RSQLW + L HEGS P + K R D+ L+LD+ LA + S ++L + D RR Q W+F+ G + + + A ++ G E AP + F RPGSG + V V+ DGPTRV+++ D I+ S + + +S+ + E+ +V EG G+ L+N PEEL++A VD+ + R + L + + IQVDNQ + + ++ +P+SS + E G V+ P ++L E K L+ T Q F E E+L+E SL + + G ++ Y NLK+ I LSV T++ LP DLK +K FP L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D+T G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ S+ T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A SS R +PRC LL
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRE-FDLPYEVKAGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPAPPQEYDEEKEREDENERKKHLLKALEDKFKCECEQKGES------YWYSVTASLVTRIVENIELKIQDVHLRFEDDSSNLENPFSFGVCINNVSAQNPAKEMVQKL-------LRQKQLEIQDFSVYWDTECEMLGKLPANQIQDAMTQCMLNR--EHQYIFEPVCASALVRRNASKEPLRSRSTPRIEGLVQLEPMSLRLSQVQYLQIMTFLKELDRRERGMFYRKWRPKVPIHTNCRQWWMFAI-EANLNVIREQRRRCSWIFSLQRARDARTYTSLYFTRLRGIPLSPLEESELKRVEEEQTFEELHTLREIVYDWF-RQQEDSTEVCFNKELYCSHSSPELSATTIQKSGSSGMIQYLQSWFPGWGGWYGDSENLVRPE----------------EPLPSPSSWSILAETEDLFDPLE--DSHTLNTFTRRDHMFARLEFLLEKGTIMLFEQDRRGPVLHESGVIHLEFSVVKVIV--ESLPRSESSLLSVKLGNLFLRDLTTQGSIFPVLMSTKTPCLYNFLLYSNPFKVESTSPVFEMIYERNPAKS----KFERRLEVRTSPLNIIYNPQAIKKVTEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKRWTMKLDICAPQVIFPD-DFQSGDPVLVVVDLGRILLTNSQDE---STISSKSAEP--EREDMSDDEYQTPLATPPESPPPELDLPLKAQLKNPEVPSLKIPESTQAYSWKLYEKYCLSFMDLQIMVGRYKDNWKHLQEGEVGPTH-------VVEKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHFNLEKMTALQSCLARLSSPAPSEADKFMEKDPNVRSPDPIPQHKYFFRDDSSLKLQGSAK--NLTQSVMTLEQHTREVLVESRLLLAEFNVAYMQLGVESEGRYISVLKVFGTKAHFVKRPYDAEVSLTVHGLLLVDTMQTYGSDFDLLVASHKHLGFDLPTGILKESQPSSPDLCNNTSEHEERLPEFSSNLSMGNTSPFSSIFKDEDALIKLEYQFVSSDCPSMNLDSSLQVTSIQVNNLDIILNPETIVELLKFLQKSFPREDSSSVQQQPTHLHNEDEDRTYQATYDQNKELTVEIHRLNLLLLRTVSTGTTLGAEKKAMKIATASIAGTKVNVSM---GSTFDVSGSLGLMQLGDLTQEGNRSQFVVSIGSVEGNSLGLD-GLTFLSDTCGSQTDALNFRLL-EQSRGECSLKLHMASLHYNHSAKFLRELSLSANELEDSFRSMLKN-AATKVSTVLASKTAEYSGMVSLFETPLKKSRTFSHSWPYSFEEEEEVPVEEPESTLDDFSVKLNLNIHIESPVVSIPRKFGHPELLVGHLGSIRICNCLNSQDLPGETLQVLVKDIRLYSLNMSHLIPKRAPRWERVDDVLRYLINQEEAPFTRNDFFESLSRGQAFHILKDTTIQFTVEKLPLDEDAEQSLSTTEESYRSVGQLRIQGEFVTPVQVFLCKPVYEQVLQTLDNLFLIEEQHTMPSQPPTPPPPTPSSVRLHWFPDPQGGLFARDPPSVNFSHSSLSSPLSLQSQKPAPYSASFTQLKVNLHVAELQVQLSADLTQGSQG-LVSVRFQDLEGDFTKDHPNSLRVQLALRAVLMEDLLEQNPESKYKYLMVSRGAPKPSTFSPKEYLSQSCPS---QSNAFYPDMPRSLPAHMEEAQNVFQLYQRHSSTPSNSNRKSKKDPECPITPPPSPLHCKASLQPSDFDDSLVHINVLLVDQNHPEFKTRYGSVGRSVDIDFNCLDVLITLQTWVVILDFFGIGSTASN-HAVKEEIP-PQAVPDFPL-YDPDVDETVQPVNTKVDLKVGNSCLNIVTQMNPCIKSCF---------IMSYIADGDMALHGSLRSLSLSDLTPHGSLYGER-FTTQGGDALV-FNILKYGQPDPHLERECDIMVSLQMA-SVQYVHTQRFQAELVAFIHHFTQLQDILGRQRAAMEGQTVRDCPQRASRVLLDIDAGAPVIVIPESSQSPRVIVANFGQLRVKNRFLPAGACGTFSLREKVEKLTMVHAFFPSLVSHTAGSSKFLLSCLLDCMALDLQEMDIFAAERFPSCLWDIGNNTESSDIVFPSYFVRRTGDNLLKDCCRLKLQVERNLDRELSHAVPDMSIHGSLSSVHCSLDRKHYQLIRGLLENNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVYTNLSFLMNMMDVSLELLES-SGQTQHRQ-SLGRFDFVKSKLLFESFSNGSKSVNLVSHSLLANDTRYSE-PNRRTDGSRPNVFDCILQPSKTGTNRA-----SLQLELHYRSTRESSCFTVLLNNLRVFLIFDWLQLVRDF----------LQMPDEKRSSG-------SKSRQRGSSFSSTDSGALGTVMPKTVKSGVVTKRSTVPVTQDHC--LEVKINVTGTEFVVVEDRSSLESNAIILKGTTVLTYKPRLLDRP--FSGSLAGVEVFSCRLGSEQETALSIIDPVNVQVD----LC---GSPTYQSSSGLLDAFNVEDIPPL-LEVRIIFFFERFSF--LPSLLQVWRQTQNHLLDLGFRREDCKRALIHCKGQLDLAATWLLENAEHVVGHSRSTADPGAGSH-SAPLSGVEI----KAENLCICFIDDCLDCDIPLAELTFSRFCVLQRIGSTQEGNASFTLSGDYYNRELSGWEPFIEPWPCSLSWQQQAAGRLHPPRFKMGVRSKQRLDVNITSVLLEQYKTTKSSWMADYCREEEHTPSSSSSPPWMGSSVDPPSFGQNVRMSKRRQP----FVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISDIHSSGVDDSHNVSQWREVLPGEELPFEFEAREKLRHRH--THELKLHQLLVRVCGWEQVKPVSVDKVGIFFRYAAPDRNSSSSTVGSPISRTNIIHPHVYFSALPPVRVVFAITMEGSARKVITVRSSLMVKNRLDVLMEVRLDSPSAPDKPMVLPPILPGQALAIPLHLTSWRLQARPKGMG------------LFFCKVPIH-----WTSVERPEEISSSKRECQSA--DYDDSHKQNFRFCV-VIKKENYPDQLPAKRQVSGSTQEIYRQPGHTIYLLPTMVLVNQLPCELNYYIK---------GVHIQGSLNPGKEAVLHLADTSQNLELGVLLESFPVCKELLIPPGTQ-NYVVRMRLYDTEKRLLCLTIRIILRAQGALKILISAPYWLINKTGLPLIFRQDNGKAD--AAGQFEEHELARSLSPLLFCYTDKEQPAMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGISVRKGKGQYRDTHIVTFAPRYLLDNRSTHKLAFAQREFARGQGTSNPEGYISTLPGSSVVFHWPRNDFDQLLCVRLMEVTN---CTWSGGFEVNKPKSFHINMRDTLGNC-FFLRTEITLKGATYQISFTNADQLPPPLRIDNLSEVPIQFWQH-GVADLRLHTEVKPMSVLDYACDEPTLPPYLTITVKGAGSSDVTADMNFFKEFNKLYYENFIYIAATHTFSQTAETRPVGKKWLMSWSELVLDVDTKTQRVILRKKEPGKRSQLWRMTGTGMLCHEGSSPPQN-KPTQPRPLDSSLVLDIAGLA---AVSDNSFEPLMLRRPDSRRSTTQTWYFSSGMLTCGLPRLVVQVKGGI--AGLYDGAEVVLGPDSGLAPPGPEQQFINQKMRPGSGVLSVQVIPDGPTRVLQISDFNQRRIIRSSASNEHEESAEDTKSRPHRQELEVTVNLEEGLGISLVNKSPEELVFATLFGVDIHFTRTVTNEVLELSVQNIQVDNQLLGSTQPVML-----CVTPNSSDCNLN-----ESGPALQVNSVK------VPSTLMLTELFKHLMVTAQRFTIIIEEKLLLKLLSFFGYGEAEAELEMLDE---SLPERPSEEAGPPKRYYFENLKISLPQIKLSVFTSQKLPPDLKALKGTLGFP-LIRFEDAVINMYPFTRVHPYETQEIIFNDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSERECIRYHGATSGEH---LVAGIHGLAHGIIGGMTSIITSTVEGVKMEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSSHRLGVQRVRKPRCCKGPQDLL 4112
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Medaka
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:NCBI gene;Acc:101163959]) HSP 1 Score: 1080.09 bits (2792), Expect = 0.000e+0 Identity = 1134/4329 (26.20%), Postives = 1910/4329 (44.12%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS-NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVL-QEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR-------ERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQ----------------NAVNIPAPQQSG-------W---WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLE-GIKLKLLKDTEIPIL--------EVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYK-------------NDSFRY-VVSSREHSKV--PGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLE-NLSMATDCQASKETKPVTNI-----------YDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTN--------FM--------------NNYRNADSSSVKYKKQTRMDNLNKSP-EINQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSES--------------------------IG------------DPIIEFTYKISQKSGSS---DIQRQINLI-FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKP---------ETPNTTEFKEFDVSIACKAVSILLIKISSN---LASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDIS----------ILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEESTRIGFLN--------------------------------------SVKLHL--RMLSPILALPRHKTSNQIILVNLGDIEITS-------DTQSLQVAFSDA---------------------------IVISKELPIENG--------GYDIGICTDNNAKF-----PLVNKIDLMLKFSK--LHDPPKILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDEI-----------------------PK--------PFIEFEDENMQED-----QNPDP-SENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIP----PPSPFYKYSKRKKSLSNS----VDIQNCAAFELKLPKNTVKSTEP----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEII---NTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEF-------KFENNDRSR-------------------------CLLDEMKIDLHEMDVYSAIRITDSILKDG------DLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANK----RLNVFPIIISPDK-NTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP-----------------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRE-NRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPA--------NLAYQTGYL--RHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQE-------------------------RNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP---LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETF---KEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNI-----EMHFSV---EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEE--KMLLKTMQMFL------------------SPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLL 11341
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L + F+ P VK+G IG++TL+IP+ R S W I + + +I GP EKE ++EN KK LL ++E K++ + Q+G++ W S ASL RI+ N+++++++VH+R+ED+S+ F+ G+ I+ S ++ E K + KQ+++ S+Y DT+C+++ ++ I+ + Q LN + I P+ +++RN + PL PRI +Q+E + + LSQ QY + E +R +R + K+RP+V + + ++WW+FAI E ++ + R +RAR+ Y Y L S LE +E + + L LRE+ + R+ D + +A I SG W WG +G PE + LP S+ S +D+ + L D +N FTR D +F R EF LE G + +D P+L E + +IV ES P+ S + + L +LF+RDL + +FR + S S V +F + +E NP S +R L + + PL I++ P I + E+F + + + AA + K +T I++ +++ + RW + L I AP++I P+ ++ +P V++DLGR+ ++N D+ +I + EP D+ +++ TP ++P S L + Q P I Y++Y + ++ +VG NW L E E+ ++E FN +L L+++ Q P ++ + L + + +K+ + CL +++ FM + Y D SS+K + + NL +S + Q+ + V + F + + +ES R ++ L + T+ + +S+ + L+L D GS D ++ASHK L D P L ES +G D +I+ Y+ S D Q+ I ++LD+I N ET+ ELL F++ F + Q+P T T + ++++ +++LL++ S L +E + ++ I+ +++N+ M G+ + + + + + D++ + S+ N L L G D +N ++ E + G + + + Y HS +FL + +E + F + N AA + +L+S+ + G ++ SVKL+L + SP++++PR ++++ +LG I I + ++LQV D ++ +E P G I D +F PL + L ++ ++ I+ EF + + + K VYEQVL+++DNL +E P+ P + F ++ Q P P S + + + ++++++++L D S+ ++ + Q + +F + + ++LA+ + + +L+ + K L+ S + + +E S SCP Q + Y ++ +L + F Y + + SNS C P + S +P + L I + L+D+ F T S+ R VD DFN L I+++ WV++LDFFG+GS + VK+ + + +P F YD +++E + NTK+++ + L + + + S + +Y DG+M L GSL L DL+P GS+Y +R F D ++ F++ KYG PDP L RE D+ + +++ + Y+HTQRF EL F +F Q QD L R G+ V QR++R+ + I AP++++P S+ S +++ + G+ + N F F ++ CLLD M +DL EMD+++A R + G D+ F +R L + C L L V RNL+ +SH PD I+G LSSV S+ + Y+ IRG+L +NLGE + L+ Y Q DP +T L+G Y + + + DV L L+ G + +L R +F +SKL +ESFS+ SK++ LV + D RYS P + R NVF I+ P K T R+ + QL + Y S + + F V LN ++ +IFDW+Q+ +++ L DE R SK + R SF V+ ++ + Q+ E+ +NV + F ++E+ S L ++A+IL +YK R +RP FS S++ + SC + S+ LSII+PV + + +C G P+ L D + ++ L +++ + F R SF + L ++ R QN + L + G L W N ++V S + A S + + I E + +C IDDC+D D+PL ++ F+ V +G+++ +F LS DYYN GW P IEPWP ++ W K + S+ L+ +I+S LL+ K T W ++ +NV R+ FVP+ + N GC + + L+ + ++ + V DS S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+GIF+R+ D + V + I R+V I +GS + I +RS L + N L++ +E + V+ + P + IPL + +Q RP LF+ + + W +V P + ++ C S+ ++ S ++N + + I++ N+P A + V PGH I + L N+LPC++ + +K + + + P + + Q+ V L+SFP C LLI + ++ V +RLYD + RLL L +++ R G + ++IS+ YW++N+T LPL++ Q +G+ + AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRS+ L F Q Q I LP S + FHWPR+D DQLL V+ ++ + SGGF+++ SFH+ +R N LR ITL+GA Y++ FT+AD LP P +++NL+ + + F+Q+ + R H+ + P S + YA DEPTLPP + + ++G S D+N LYY+NF+YIA ++F + LV DV ++V L +K RSQLW + L HEGS P + K R D+ L+LD+ LA + S ++L + D RR Q W+F+ G + + + A ++ G E AP + F RPGSG + V V+ DGPTRV+++ D I+ S + + +S+ + E+ +V EG G+ L+N PEEL++A VD+ + R + L + + IQVDNQ + + ++ +P+SS + E G V+ P ++L E K L+ T Q F E E+L+E SL + + G ++ Y NLK+ I LSV T++ LP DLK +K FP L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D+T G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ S+ T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A SS R +PRC LL
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRE-FDLPYEVKAGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPAPPQEYDEEKEREDENERKKHLLKALEDKFKCECEQKGES------YWYSVTASLVTRIVENIELKIQDVHLRFEDDSSNLENPFSFGVCINNVSAQNPAKEMVQKL-------LRQKQLEIQDFSVYWDTECEMLGKLPANQIQDAMTQCMLNR--EHQYIFEPVCASALVRRNASKEPLRSRSTPRIEGLVQLEPMSLRLSQVQYLQIMTFLKELDRRERGMFYRKWRPKVPIHTNCRQWWMFAI-EANLNVIREQRRRCSWIFSLQRARDARTYTSLYFTRLRGIPLSPLEESELKRVEEEQTFEELHTLREIVYDWF-RQQEDSTEVCFNKELYCSHSSPELSATTIQKSGSSGMIQYLQSWFPGWGGWYGDSENLVRPE----------------EPLPSPSSWSILAETEDLFDPLE--DSHTLNTFTRRDHMFARLEFLLEKGTIMLFEQDRRGPVLHESGVIHLEFSVVKVIV--ESLPRSESSLLSVKLGNLFLRDLTTQGSIFPVLMSTKTTPLHVTFRLQIFGSSYTSGVVESTSPVFEMIYERNPAKS----KFERRLEVRTSPLNIIYNPQAIKKVTEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKRWTMKLDICAPQVIFPD-DFQSGDPVLVVVDLGRILLTNSQDE---STISSKSAEP--EREDMSDDEYQTPLATPPESPPPELDLPLKAQLKNPEVPSLKIPESTQAYSWKLYEKYCLSFMDLQIMVGRYKDNWKHLQEGEVGPTH-------VVEKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHFNLEKMTALQSCLARLSSPAPSEADKFMEKDPNVRSPDPIPQHKYFFRDDSSLKLQGSAK--NLTQSVMTLEQHTREVLVESRLLLAEFNVAYMQLGVESEGRYISVLKVFGTKAHFVKRPYDAEVSLTVHGLLLVDTMQTYGSDFDLLVASHKHLGFDLPTGILKESQPSSPDLCNNTSEHEERLPEFSSNLSMGNTSPFSSIFKDEDALIKLEYQFVSSDCPSMNLDSSLQVTSIQVNNLDIILNPETIVELLKFLQKSFPREDSSSVQQQPTHLHNEDEDRTYQATYDQNKELTVEIHRLNLLLLRTVSTGTTLGAEKKAMKIATASIAGTKVNVSM---GSTFDVSGSLGLMQLGDLTQEGNRSQFVVSIGSVEGNSLGLD-GLTFLSDTCGSQTDALNFRLL-EQSRGECSLKLHMASLHYNHSAKFLRELSLSANELEDSFRSMLKN-AATKVSTVLASKTAEYSGMVSLFETPLKKSRTFSHSWPYSFEEEEEVPVEEPESTLDDFSVKLNLNIHIESPVVSIPRKFGHPELLVGHLGSIRICNCLNSQDLPGETLQVLVKDIRLYSLNMSHLIPKRAPRWERVDDVLRYLINQEEAPFTRNDFFESLSRGQAFHILKDTTIQFTVEKLPLDEDAEQSLSTTEESYRSVGQLRIQGEFVTPVQVFLCKPVYEQVLQTLDNLFLIEEQHTMPSQPPTPPPPTPSSVRLHWFPDPQGGLFARDPPSVNFSHSSLSSPLSLQSQKPAPYSASFTQLKVNLHVAELQVQLSADLTQGSQG-LVSVRFQDLEGDFTKDHPNSLRVQLALRAVLMEDLLEQNPESKYKYLMVSRGAPKPSTFSPKEYLSQSCPS---QSNAFYPDMPRSLPAHMEEAQNVFQLYQRHSSTPSNSNRKSKKDPECPITPPPSPLHCKASLQPSDFDDSLVHINVLLVDQNHPEFKTRYGSVGRSVDIDFNCLDVLITLQTWVVILDFFGIGSTASN-HAVKEEIP-PQAVPDFPL-YDPDVDETVQPVNTKVDLKVGNSCLNIVTQMNPCIKSCF---------IMSYIADGDMALHGSLRSLSLSDLTPHGSLYGER-FTTQGGDALV-FNILKYGQPDPHLERECDIMVSLQMA-SVQYVHTQRFQAELVAFIHHFTQLQDILGRQRAAMEGQTVRDCPQRASRVLLDIDAGAPVIVIPESSQSPRVIVANFGQLRVKNRFLPAGACGTFSLREKVEKLTMVHAFFPSLVSHTAGSSKFLLSCLLDCMALDLQEMDIFAAERFPSCLWDIGNNTESSDIVFPSYFVRRTGDNLLKDCCRLKLQVERNLDRELSHAVPDMSIHGSLSSVHCSLDRKHYQLIRGLLENNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVYTNLSFLMNMMDVSLELLES-SGQTQHRQ-SLGRFDFVKSKLLFESFSNGSKSVNLVSHSLLANDTRYSE-PNRRTDGSRPNVFDCILQPSKTGTNRA-----SLQLELHYRSTRESSCFTVLLNNLRVFLIFDWLQLVRDF----------LQMPDEKRSSG-------SKSRQRGSSFSSTDSGALGTVMPKTVKSGVVTKRSTVPVTQDHC--LEVKINVTGTEFVVVEDRSSLESNAIILKGTTVLTYKPRLLDRP--FSGSLAGVEVFSCRLGSEQETALSIIDPVNVQVD----LC---GSPTYQSSSGLLDAFNVEDIPPL-LEVRIIFFFERFSF--LPSLLQVWRQTQNHLLDLGFRREDCKRALIHCKGQLDLAATWLLENAEHVVGHSRSTADPGAGSH-SAPLSGVEI----KAENLCICFIDDCLDCDIPLAELTFSRFCVLQRIGSTQEGNASFTLSGDYYNRELSGWEPFIEPWPCSLSWQQQAAGRLHPPRFKMGVRSKQRLDVNITSVLLEQYKTTKSSWMADYCREEEHTPSSSSSPPWMGSSVDPPSFGQNVRMSKRRQP----FVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISDIHSSGVDDSHNVSQWREVLPGEELPFEFEAREKLRHRH--THELKLHQLLVRVCGWEQVKPVSVDKVGIFFRYAAPDRNSSSSTVGSPISRTNIIHPHVYFSALPPVRVVFAITMEGSARKVITVRSSLMVKNRLDVLMEVRLDSPSAPDKPMVLPPILPGQALAIPLHLTSWRLQARPKGMG------------LFFCKVPIH-----WTSVERPEEISSSKRECQSA--DYDDSHKQNFRFCV-VIKKENYPDQLPAKRQVSGSTQEIYRQPGHTIYLLPTMVLVNQLPCELNYYIK---------GVHIQGSLNPGKEAVLHLADTSQNLELGVLLESFPVCKELLIPPGTQ-NYVVRMRLYDTEKRLLCLTIRIILRAQGALKILISAPYWLINKTGLPLIFRQDNGKAD--AAGQFEEHELARSLSPLLFCYTDKEQPAMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGISVRKGKGQYRDTHIVTFAPRYLLDNRSTHKLAFAQREFARGQGTSNPEGYISTLPGSSVVFHWPRNDFDQLLCVRLMEVTN---CTWSGGFEVNKPKSFHINMRDTLGNC-FFLRTEITLKGATYQISFTNADQLPPPLRIDNLSEVPIQFWQH-GVADLRLHTEVKPMSVLDYACDEPTLPPYLTITVKGAGSSDVTADMNFFKEFNKLYYENFIYIAATHTFSQTAETRPVGKKWLMSWSELVLDVDTKTQRVILRKKEPGKRSQLWRMTGTGMLCHEGSSPPQN-KPTQPRPLDSSLVLDIAGLA---AVSDNSFEPLMLRRPDSRRSTTQTWYFSSGMLTCGLPRLVVQVKGGI--AGLYDGAEVVLGPDSGLAPPGPEQQFINQKMRPGSGVLSVQVIPDGPTRVLQISDFNQRRIIRSSASNEHEESAEDTKSRPHRQELEVTVNLEEGLGISLVNKSPEELVFATLFGVDIHFTRTVTNEVLELSVQNIQVDNQLLGSTQPVML-----CVTPNSSDCNLN-----ESGPALQVNSVK------VPSTLMLTELFKHLMVTAQRFTIIIEEKLLLKLLSFFGYGEAEAELEMLDE---SLPERPSEEAGPPKRYYFENLKISLPQIKLSVFTSQKLPPDLKALKGTLGFP-LIRFEDAVINMYPFTRVHPYETQEIIFNDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSERECIRYHGATSGEH---LVAGIHGLAHGIIGGMTSIITSTVEGVKMEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSSHRLGVQRVRKPRCCKGPQDLL 4118
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Medaka
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:NCBI gene;Acc:101163959]) HSP 1 Score: 1072.77 bits (2773), Expect = 0.000e+0 Identity = 1145/4361 (26.26%), Postives = 1917/4361 (43.96%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNS-NWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVL-QEKLNEMPKSDCIVNPISLHGIMKRNIN--PLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALR-------ERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAV------NIPAPQQSG----------W---WGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTR-DKIFLRGEFSLEGIKLKLLKD-----TEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGE---------IFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTEN-------------IKAAAEKHIRNAKARTSESIKKILEKG------SSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLE-NLSMATDCQASKETKPVTNI-----------YDEYSIKLENVMALVG----NWNEL-ESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFM---NNYRNADSSSVKYKKQTRMDNLNKSP-EINQNYKSVSIS-------FVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQP---LSES--------------------------IG------------DPIIEFTYKISQKSGSS---DIQRQINLI-FSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKP---------ETPNTTEFKEFDVSIACKAVSILLIKISSN---LASENR-FAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDIS----------ILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEESTRIGFLN--------------------------------------SVKLHL--RMLSPILALPRHKTSNQIILVNLGDIEITS-------DTQSLQVAFSDA---------------------------IVISKELPIENG--------GYDIGICTDNNAKF-----PLVNKIDLMLKFSK--LHDPPKILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDE---------------------IPKPFIEFEDENMQEDQNPDP-SENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEK-SVSCPDLLEQLESSYSNLTSTLIP----PPSPFYKYSKRKKSLSNS----VDIQNCAAFELKLPKNTVKSTEP----NKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEII---NTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKI-----------SCQAPLLL-------------LPVSA---FS-----------------DHMLIVDLGKFSLVNEFKFENNDR--------------------SRCLLDEMKIDLHEMDVYSAIRITDSILKDG------DLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDH-LKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANK----RLNVFPIIISPDK-NTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQP-----------------VIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTR-ENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPS------LDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKP----SQPNLTALQKLS---KFVDRISIHH-----------LDDYEFV---------------------------SVCL--IDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAF-LSVDYYNTNSFGWTPVIEPWPLTICWFWLTTK-------KFSLTSEVTLNADISSHLLQIAKNTHKKWSTEF---------------------------QERNVAFCDCRKRHN--NL-----------------FVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDM--------ETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYI------------------RIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVV--LKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFP---LAPKMV----------PGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQ-SGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQ--NRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKP-----IMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQS--CYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNL------------LVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETF---KEFYAPLVYKNWF-----RPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSV---EGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEE--KMLLKTMQMFL------------------SPSKEAELLEEDFSSLQHQSHFDEG--RKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKL--FPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGP-SGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIAR-SSKEGQPPRRYEPRCLSSSWKLL 11341
M+ L+ ++L+ +GKYV N +T + L KG + LENLPL+ L + F+ P VK+G IG++TL+IP+ R S W I + + +I GP EKE ++EN KK LL ++E K++ + Q+G++ W S ASL RI+ N+++++++VH+R+ED+S+ F+ G+ I+ S ++ E K + KQ+++ S+Y DT+C+++ ++ I+ + Q LN + I P+ +++RN + PL PRI +Q+E + + LSQ QY + E +R +R + K+RP+V + + ++WW+FAI E ++ + R +RAR+ Y Y L + E + +E + + L LRE+ + R+ D + +V + Q+SG W WG +G PE + LP S+ +E +D+ + L D +N FTR D +F R EF LE + L + + L K ++ ES P+ S + + L +LF+RDL + F ++S++ V G E +F + +E NP S +R L + + PL I++ P I + E+F + + + AA + K +T I++ +++ + RW + L I AP++I P+ ++ +P V++DLGR+ ++N D+ +I + EP D+ +++ TP ++P S L + Q P I Y++Y + ++ +VG NW L E E+ ++E FN +L L+++ Q P ++ + L + + +K+ + CL +++ + Y D SS+K + + NL +S + Q+ + V + F + + +ES R ++ L + T+ + +S+ + L+L D GS D ++ASHK L D P L ES +G D +I+ Y+ S D Q+ I ++LD+I N ET+ ELL F++ F + Q+P T T + ++++ +++LL++ S L +E + ++ I+ +++N+ M G+ + + + + + D++ + S+ N L L G D +N ++ E + G + + + Y HS +FL + +E + F + N AA + +L+S+ + G ++ SVKL+L + SP++++PR ++++ +LG I I + ++LQV D ++ +E P G I D +F PL + L ++ ++ I+ EF + + + K VYEQVL+++DNL +E P P + Q P P S + + + ++++++++L D S+ ++ + Q + +F + + ++LA+ + + +L+ + K L+ S + + +E S SCP Q + Y ++ +L + F Y + + SNS C P + S +P + L I + L+D+ F T S+ R VD DFN L I+++ WV++LDFFG+GS + VK+ + + +P F YD +++E + NTK+++ + SL+L + +N A VS + L+ DG+M L GSL L DL+P GS+Y +R F D ++ F++ KYG PDP L RE D+ + +++ + Y+HTQRF EL F +F Q QD L R G+ V QR++R+ + I S Q+P ++ LP A FS L + L S+ +E K N + CLLD M +DL EMD+++A R + G D+ F +R L + C L L V RNL+ +SH PD I+G LSSV S+ + Y+ IRG+L +NLGE + L+ Y Q DP +T L+G Y + + + DV L L+ ++ + +F +SKL +ESFS+ SK++ LV + D RYS P + R NVF I+ P K T R+ + QL + Y S + + F V LN ++ +IFDW+Q+ +++ L DE R SK + R SF V+ ++ + Q+ E+ +NV + F ++E+ S L ++A+IL +YK R +R PFS S++ + SC + S+ LSII+PV + + +C G P+ L D + ++ L ++I A IRLS++DIQL I +SF P A+ S + D V+ S P +P L+ L L + R IH L++ E V ++C+ IDDC+D D+PL ++ F+ V +G+++ +F LS DYYN GW P IEPWP ++ W K + S+ L+ +I+S LL+ K T W ++ Q +A R N NL FVP+ + N GC + + L+ + ++ + V DS S + ++ E AR+K+R+ +H H++ +++ GW + +PV+VDK+GIF+R+ D + V + I R+V I +GS + I +RS L + N L++ +E + V+ + P + IPL + +Q RP LF+ + + W +V P + ++ C S + ++ S ++N + + I++ N+P A + V PGH I + L N+LPC++ + +K + + + P + + Q+ V L+SFP C LLI + ++ V +RLYD + RLL L +++ R G + ++IS+ YW++N+T LPL++ Q +G+ + AAG E+E A S P F +++K +R+G + P W + SL+ +RA V Q NRP L+YNIG+ +R G+G++ +T +V RYLLDNRS+ L F Q Q I LP S + FHWPR+D DQLL V+ ++ + SGGF+++ SFH+ +R N LR ITL+GA Y++ FT+AD LP P +++NL+ + + F+Q+ + R H+ + P S + YA DEPTLPP + + ++G S D+N LYY+NF+YIA ++F + LV DV ++V L +K RSQLW + L HEGS P + K R D+ L+LD+ LA + S ++L + D RR Q W+F+ G + + + A ++ G E AP + F RPGSG + V V+ DGPTRV++ + +L ++ + N F +E +V EG G+ L+N PEEL++A VD+ + R + L + + IQVDNQ + + ++ +P+SS + E G V+ P ++L E K L+ T Q F E E+L+E SL + + G ++ Y NLK+ I LSV T++ LP DLK +K FP L+ F +A I + + R H +ET +I I H+R +L SQA +I GSVDFLGNP+G ++D+T G+ L +V ++++ HG+ +S AK + S + + TMD ++ +R+ + + SG + + AG LA+GI GG+ S+ T +G K EG SG F G KG+VG VTKPV G LD A+ +A + R++A SS R +PRC LL
Sbjct: 1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRE-FDLPYEVKAGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPAPPQEYDEEKEREDENERKKHLLKALEDKFKCECEQKGES------YWYSVTASLVTRIVENIELKIQDVHLRFEDDSSNLENPFSFGVCINNVSAQNPAKEMVQKL-------LRQKQLEIQDFSVYWDTECEMLGKLPANQIQDAMTQCMLNR--EHQYIFEPVCASALVRRNASKEPLRSRSTPRIEGLVQLEPMSLRLSQVQYLQIMTFLKELDRRERGMFYRKWRPKVPIHTNCRQWWMFAI-EANLNVIREQRRRCSWIFSLQRARDARTYTSLYFTRLRGIPLSPLEELKR-VEEEQTFEELHTLREIVYDWF-RQQEDSTEPSVECQPELSATTIQKSGSSGMIQYLQSWFPGWGGWYGDSENLVRPE----------------EPLPSPSSWTET---EDLFDPLE--DSHTLNTFTRRDHMFARLEFLLEKGTIMLFEQDRREKNSVFFLSSVVKVIV---ESLPRSESSLLSVKLGNLFLRDLT--TQGSIFPVLMSTK---TVSGSERELFESTSPVFEMIYERNPAKS----KFERRLEVRTSPLNIIYNPQAIKKVTEFFYKGRVHTSGFGYQSELELRVAEAARRQYNKLKMQTKAEIRQTIDQLLVGEFIENSKRWTMKLDICAPQVIFPD-DFQSGDPVLVVVDLGRILLTNSQDE---STISSKSAEP--EREDMSDDEYQTPLATPPESPPPELDLPLKAQLKNPEVPSLKIPESTQAYSWKLYEKYCLSFMDLQIMVGRYKDNWKHLQEGEVGPTH-------VVEKFNVLLQLEQRLRYTSDPQLPGAVLSGTLPDLKVHFNLEKMTALQSCLARLSSPAPSEHKYFFRDDSSLKLQGSAK--NLTQSVMTLEQHTREVLVESRLLLAEFNVAYMQLGVESEGRYISVLKVFGTKAHFVKRPYDAEVSLTVHGLLLVDTMQTYGSDFDLLVASHKHLGFDLPTGILKESQPSSPDLCNNTSEHEERLPEFSSNLSMGNTSPFSSIFKDEDALIKLEYQFVSSDCPSMNLDSSLQVTSIQVNNLDIILNPETIVELLKFLQKSFPREDSSSVQQQPTHLHNEDEDRTYQATYDQNKELTVEIHRLNLLLLRTVSTGTTLGAEKKAMKIATASIAGTKVNVSM---GSTFDVSGSLGLMQLGDLTQEGNRSQFVVSIGSVEGNSLGLD-GLTFLSDTCGSQTDALNFRLL-EQSRGECSLKLHMASLHYNHSAKFLRELSLSANELEDSFRSMLKN-AATKVSTVLASKTAEYSGMVSLFETPLKKSRTFSHSWPYSFEEEEEVPVEEPESTLDDFSVKLNLNIHIESPVVSIPRKFGHPELLVGHLGSIRICNCLNSQDLPGETLQVLVKDIRLYSLNMSHLIPKRAPRWERVDDVLRYLINQEEAPFTRNDFFESLSRGQAFHILKDTTIQFTVEKLPLDEDAEQSLSTTEESYRSVGQLRIQGEFVTPVQVFLCKPVYEQVLQTLDNLFLIEEQHTMPSQPPTPPPPTPSSVRLHWFPDPQGGLFARDPPSSQKPAPYSASFTQLKVNLHVAELQVQLSADLTQGSQG-LVSVRFQDLEGDFTKDHPNSLRVQLALRAVLMEDLLEQNPESKYKYLMVSRGAPKPSTFSPKEYLSQSCPS---QSNAFYPDMPRSLPAHMEEAQNVFQLYQRHSSTPSNSNRKSKKDPECPITPPPSPLHCKASLQPSDFDDSLVHINVLLVDQNHPEFKTRYGSVGRSVDIDFNCLDVLITLQTWVVILDFFGIGSTASN-HAVKEEIP-PQAVPDFPL-YDPDVDETVQPVNTKVDLKVHSLSLVLNKK---LNELARASVSKLSAHLEML--DGDMALHGSLRSLSLSDLTPHGSLYGER-FTTQGGDALV-FNILKYGQPDPHLERECDIMVSLQMA-SVQYVHTQRFQAELVAFIHHFTQLQDILGRQRAAMEGQTVRDCPQRASRVLLDIDAGAPVIVIPESSQSPRVIVANFGQLRVKNRFLPAGACGTFSLREKETLKHSLRKLRRKHKYLPLPLWHISISSELKLTLNSCQTLLVLKSLQNKPHLMMFFCAACLLDCMALDLQEMDIFAAERFPSCLWDIGNNTESSDIVFPSYFVRRTGDNLLKDCCRLKLQVERNLDRELSHAVPDMSIHGSLSSVHCSLDRKHYQLIRGLLENNLGEPVEEFLRPYNLQ----DPSSYTVLSGDVYTNLSFLMNMMDVSLELL------ESSGQTQHRQFDFVKSKLLFESFSNGSKSVNLVSHSLLANDTRYSE-PNRRTDGSRPNVFDCILQPSKTGTNRA-----SLQLELHYRSTRESSCFTVLLNNLRVFLIFDWLQLVRDF----------LQMPDEKRSS-------GSKSRQRGSSFSSTDSGALGTVMPKTVKSGVVTKRSTVPVTQDHC--LEVKINVTGTEFVVVEDRSSLESNAIILKGTTVLTYKPRLLDR--PFSGSLAGVEVFSCRLGSEQETALSIIDPVNVQV----DLC---GSPTYQSSSGLLDAFNVEDIPPL-LEIQFPALDIRLSYNDIQLFLAIAKSF-----PTASSIL----------SPDTDRVDASPPLSDGFKPQLSHLLDLGFRREDCKRALIHCKGQLDLAATWLLENAEHVVGHSRSTADPGAGSHSAPLSGVEIKAENLCICFIDDCLDCDIPLAELTFSRFCVLQRIGSTQEGNASFTLSGDYYNRELSGWEPFIEPWPCSLSWQQQAAGRLHPPRFKMGVRSKQRLDVNITSVLLEQYKTTKSSWMADYCREEEHTPSSSSSPPWMGSSVDPPSFGQSAPLAHLRTRSAVNMVNLQQQVHYTGAVMSKRRQPFVPYALRNHTGCTMWFATLTTTPTRVALSHSSSADSISDIHSSGVDDSHNVSQWREVLPGEELPFEFEAREKLRHRH--THELKLHQLLVRVCGWEQVKPVSVDKVGIFFRYAAPDRNSSSSTVGSPISRTNIIHPHVYFSALPPVRVVFAITMEGSARKVITVRSSLMVKNRLDVLMEVRLDSPSAPDKPMVLPPILPGQALAIPLHLTSWRLQARPKGMG------------LFFCKVPI-----HWTSVERPEEISSSKRECQS--ADYDDSHKQNFRFCV-VIKKENYPDQLPAKRQVSGSTQEIYRQPGHTIYLLPTMVLVNQLPCELNYYIK---------GVHIQGSLNPGKEAVLHLADTSQNLELGVLLESFPVCKELLIPPGTQ-NYVVRMRLYDTEKRLLCLTIRIILRAQGALKILISAPYWLINKTGLPLIFRQDNGKAD--AAGQFEEHELARSLSPLLFCYTDKEQPAMCTMRIGKGIHPDGV----PGWCQGFSLDGGSG----VRAVKVIQHGNRPGLIYNIGISVRKGKGQYRDTHIVTFAPRYLLDNRSTHKLAFAQREFARGQGTSNPEGYISTLPGSSVVFHWPRNDFDQLLCVRLMEVTN---CTWSGGFEVNKPKSFHINMRDTLGN-CFFLRTEITLKGATYQISFTNADQLPPPLRIDNLSEVPIQFWQH-GVADLRLHTEVKPMSVLDYACDEPTLPPYLTITVKGAGSSDVTADMNFFKEFNKLYYENFIYIAATHTFSQTAETRPVGKKWLMSWSELVLDVDTKTQRVILRKKEPGKRSQLWRMTGTGMLCHEGSSPPQN-KPTQPRPLDSSLVLDIAGLA---AVSDNSFEPLMLRRPDSRRSTTQTWYFSSGMLTCGLPRLVVQVKGGI--AGLYDGAEVVLGPDSGLAPPGPEQQFINQKMRPGSGVLSVQVIPDGPTRVLQKRISNELLLLCRNSVLENDTGFCFFRLET-VTVNLEEGLGISLVNKSPEELVFATLFGVDIHFTRTVTNEVLELSVQNIQVDNQLLGSTQPVML-----CVTPNSSDCNLN-----ESGPALQVNSVK------VPSTLMLTELFKHLMVTAQRFTIIIEEKLLLKLLSFFGYGEAEAELEMLDE---SLPERPSEEAGPPKRYYFENLKISLPQIKLSVFTSQKLPPDLKALKGTLGFP-LIRFEDAVINMYPFTRVHPYETQEIIFNDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIKYGNVGGLIRNVTHGVSNSAAKFAGTLSDGLGK-TMDNRHQSERECIRYHGATSGEH---LVAGIHGLAHGIIGGMTSIITSTVEGVKMEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSSHRLGVQRVRKPRCCKGPQDLL 4135
BLAST of Vacuolar protein sorting 13 homolog D vs. Planmine SMEST
Match: SMESG000021175.1 (SMESG000021175.1) HSP 1 Score: 7952.82 bits (20634), Expect = 0.000e+0 Identity = 3921/3931 (99.75%), Postives = 3926/3931 (99.87%), Query Frame = 2
Query: 23 MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVGNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLSESIGDPIIEFTYKISQKSGSSDIQRQINLIFSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKEFDVSIACKAVSILLIKISSNLASENRFAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEESTRIGFLNSVKLHLRMLSPILALPRHKTSNQIILVNLGDIEITSDTQSLQVAFSDAIVISKELPIENGGYDIGICTDNNAKFPLVNKIDLMLKFSKLHDPPKILIECEXXXXXXXXXXKAVYEQVLKSMDNLVYDDEDEIPKPFIEFEDENMQEDQNPDPSENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEKSVSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNSVDIQNCAAFELKLPKNTVKSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRXXXXXXXXXXXXXXYQDRLSHIRVTSAGRIVDKSAQRSARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFSFSVSQFHLASCVMXXXXXXXXXXXXPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIXXXXXXXXXXXVVLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSFESDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRMILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPLVYKNWFRPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPYQTSYSNNLRRTPLSDLTQAQHXXXXXXXXXXXVETVVGFLIIQNSKESQLKVIVSDASIHIIKTVSGESDLLKHYPLSKLSIPPISVHSKSFIQSSPYCKNSVYVNFNYLRDMREHQITPKPPFKCDTVLIAKQLVKLVCEAHCDYLERQMTYSAG 11815
MINQLITYLLD+VIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILV+SGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMS+VSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIPILEVNA+NLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFR VVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKT EPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVGNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLSESIGDPIIEFTYKISQKSGSSDIQRQINLIFSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKEFDVSIACKAVSILLIKISSNLASENRFAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEESTRIGFLNSVKLHLRMLSPILALPRHKTSNQIILVNLGDIEITSDTQSLQVAFSDAIVISKELPIENGGYDIGICTDNNAKFPLVNKIDLMLKFSKLHDPPKILIECEFSSSLSLSFSKAVYEQVLKSMDNLVYDDEDEIPKPFIEFEDENMQEDQNPDPSENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEKSVSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNSVDIQNCAAFELKLPKNTVKSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRFLQELNQFFQNFLQYQDRLSHIRVTSAGRIVDKSAQRSARIRMKI CQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFSFSVSQFHLASCVMSSDSSNLLSIINPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIFSEKFSSFSSSVVLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISS YWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNS ESDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHR+ILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPLVYKNWFRPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPYQTSYSNNLRRTPLSDLTQAQHLLALFSASSSSVETVVGFLIIQNSKESQLKVIVSDASIHIIKTVSGESDLLKHYPLSKLSIPPISVHSKSFIQSSPYCKNSVYVNFNYLRDMREHQITPKPPFKCDTVLIAKQLVKLVCEAHCDYLERQMTYSAG
Sbjct: 1 MINQLITYLLDKVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVRSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLFEKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWESKTELTTELDVLNKQVDLSGISIYLDTKCKIMSQVSVENIKSVLQEKLNEMPKSDCIVNPISLHGIMKRNINPLPMNHQPRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFAITEHTRHIFKGAALRERARNLVKYVQSYEDYLLNKDSNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQGLEQDITELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDTEIPILEVNAENLIVGFESRPQYSSWCIRIGLRSLFVRDLNPDYKNDSFRDVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTENIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLKISAPKIILPNFRLKTDEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALVGNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLELTKGCLESVTNFMNNYRNADSSSVKYKKQTRMDNLNKSPEINQNYKSVSISFVLHSFIIQLESGSRPLAELSLTKTQFKLSRYSSMTIISVQLLNLILADARANLGSKLDFILASHKCLHSDQPLSESIGDPIIEFTYKISQKSGSSDIQRQINLIFSDLDVIANQETLTELLSFMRLLFSNDSLQIFDQKPETPNTTEFKEFDVSIACKAVSILLIKISSNLASENRFAVSVGRISLSQMNLLMHKNGTALNSTLRIRSINVEDISILDSLHKNILTLTTGSNETKMEISENEDVINISVIQENANGPFFVSGTLPHIIYVHSPEFLHSVGQCISEFQIYFSRLKHNIAAVARTAILSSEESTRIGFLNSVKLHLRMLSPILALPRHKTSNQIILVNLGDIEITSDTQSLQVAFSDAIVISKELPIENGGYDIGICTDNNAKFPLVNKIDLMLKFSKLHDPPKILIECEFSSSLSLSFSKAVYEQVLKSMDNLVYDDEDEIPKPFIEFEDENMQEDQNPDPSENEIPIVLQSNISQIKMELYPDSFDSSEDPMLRLLLQGITLEFVRANKYIKDIELAVDQISLYQVLDSRQKRIEKLLIYSFSSSNIGQENLQEKSVSCPDLLEQLESSYSNLTSTLIPPPSPFYKYSKRKKSLSNSVDIQNCAAFELKLPKNTVKSTEPNKLASIKIRLIDKKFHGFSTECDSISRFVDADFNQLTFRISMEAWVILLDFFGLGSRFYDPKDVKDLLDVSKVLPSFNRRYDGEIEEIINTKLNIHMKRFSLILENETHYINHSNLAGVSTIDFDLQAYTKDGNMTLSGSLSDFQLLDLSPTGSIYNQRLFPASNQDRILEFHLFKYGLPDPSLLREEDMRLRAKISVPIVYIHTQRFLQELNQFFQNFLQYQDRLSHIRVTSAGRIVDKSAQRSARIRMKIFCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKSYKFQAFQTDPILHTDLTGIAYPAMKIELCLNDVQLNLMSRLQGIDNYANINLCRLNFYQSKLFYESFSDSSKTIELVCQQITVEDMRYSSFPANKRLNVFPIIISPDKNTLRSEDEIDNCQLRIKYSSDNDKNSFIVHLNKMKAIMIFDWVQITKNWIFTAPYGIVSLATDDENRFDQHVAFYIQSKPQSRSISFQPVIMRKHYQEIIQEEFKPTELTLNVQESRFFILENSSQLHTHALILIVAGTCSYKTRENRPGPFSFSVSQFHLASCVMSSDSSNLLSIINPVKIIIKSEEKICVIHGKPSLDDVLHDKESTKLGVDIAVSAFKIRLSFSDIQLLGKIIRSFQNQTIPPANLAYQTGYLRHRWSSLNFDNVNTSKPSQPNLTALQKLSKFVDRISIHHLDDYEFVSVCLIDDCIDADVPLFDIKFNNVTVDIELGNSKGLMKAFLSVDYYNTNSFGWTPVIEPWPLTICWFWLTTKKFSLTSEVTLNADISSHLLQIAKNTHKKWSTEFQERNVAFCDCRKRHNNLFVPFLIVNELGCPIEYRKLSLDNPPMYNEIWNANKCGDENKWISVPDSTPASLFFDMETTARDKIRYDKLGSHFNTSHRIELKIEGWSKCQPVTVDKLGIFYRHLQSDTDDGLDLVSTYIRIVIEIVRQGSVQNKIIIRSPLQLCNSLNIPLEAIFSEKFSSFSSSVVLKPNTFIPIPLLYQTHDIQIRPLSPTHDKPVSPSSIDCLFYSPIELSENIFSWCTVNAPGQKIETEVFCVSSKSSFNASQEKNKVYLICTIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSCQCTVLTTSMKQSTYFSVYLDSFPRCDPLLIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSLYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQLSNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNEDQNKPIMALPASFLTFHWPRDDLDQLLTVQKLDSNHQPISKVSGGFQIDLCNSFHVYLRGINNNPPSQLRVTITLQGARYRVVFTDADDLPSPYKLENLTNLQVFFYQNVSESSCRKHSVLPPKSCIPYALDEPTLPPIINLCIEGGTQSCYDINKVGPGQNLYYDNFLYIAIRNSLESDLDNLLVFDVAPNCKKVFLNQKSSVSRSQLWYYSSNCELFHEGSIAPHDPKQKLDRSADNHLLLDVDILAPCPSSSQKQMHRIILAKADPRREKYQRWFFTEGGILVNKLGMKCYINASCKNAAVFGGFETFKEFYAPLVYKNWFRPGSGEVLVSVVTDGPTRVMRLIDKEDESILSQSQLDFNADSSTFSNIEMHFSVEGFGVFLINHIPEELLYAVCNNVDVVWRRQSNKDNLLVIIGKIQVDNQQMDAGYACVMYEENKSESPSSSQNAFSLKFQRELGHPYDVHVFSFIRMHIAPMCIILEEKMLLKTMQMFLSPSKEAELLEEDFSSLQHQSHFDEGRKIYIGNLKLLTGVISLSVITAESLPSDLKQMKKLFPSLMSFTNAQIIIDVYDRNHVFETGNLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQDVYNMVKHFAHGIGDSTAKICSSASRVISEITMDPEYEEKRQRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEGQPPRRYEPRCLSSSWKLLPYQTSYSNNLRRTPLSDLTQAQHLLALFSASSSSVETVVGFLIIQNSKESQLKVIVSDASIHIIKTVSGESDLLKHYPLSKLSIPPISVHSKSFIQSSPYCKNSVYVNFNYLRDMREHQITPKPPFKCDTVLIAKQLVKLVCEAHCDYLERQMTYSAG 3931
BLAST of Vacuolar protein sorting 13 homolog D vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1) HSP 1 Score: 171.4 bits (433), Expect = 1.239e-41 Identity = 216/876 (24.66%), Postives = 385/876 (43.95%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWES---KTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLN----EMPKSDCIVNPISLHGIMKRNINPLPMNHQ-PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFA----ITEHTRHIFKG------AALRERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQG-LEQDI--TELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDT--EIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-NPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTE----NIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLK--ISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALV----GNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLE 2539
++ L+ L+++ +G YV +K GL G+ LENL +K +D F+ P+ V+ G I ++TL+IP+ S+ ++ +Y++ PNS K EKE K+ L+ IE K+++ + E K S+ L +I++NLQI + N+H+RYED ST P++ FA G+ + S ++ D S K + T E K V + +++Y ++ K S + +K++++ ++ ++ ++ PIS +M ++ P N PR+SL L++EQ+ + ++ QY D +R + KYRP V + + WW F + E R + A R R R Y +E LL+K S E + E +L+L + R+ A E +K R L + GW+ +G+ ETS S S+ S L+K + + E+ L Q I +EL + D + + L+ L+ +K + ++ E ++++ ++ + R + + + + ++ V PD S +VS++ SK+ + + FETNPL + I I +EPL I++ + +I ++ + E I I K T+ ++ + +D R + +K I ILP T + +L+DLG + + + M P + ++DL FG L + KET YD++ I L + AL+ +W +L S+ +SKN ++ PF F +L KKCV+ + P I ++ + L + ISD+ L+
Sbjct: 1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDD-FDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIE---ENKLKRNDPKKETETKD--SFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTRE---FFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCR---LYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQA-EMKAKKMRKKLNQKTS-----SGGWFSGWFGKSDTETS------SSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPI------EYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPD---GSVPTLVSTQAQSKLMN--LLIVDFETNPLDKSANQKIN----IRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHL-----ADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSM-----------PGSGDTDL-----------IFGKDGKL----NYDVMKETA-----YDKFHIILSTIQALMIEQGDDWRKLRSQ---ESSKNH---ILRPFGFEFNL-KKCVITNDANLPKIFVKGCLPHLGVIISDRVLQ 794 HSP 2 Score: 69.3218 bits (168), Expect = 8.463e-11 Identity = 62/207 (29.95%), Postives = 99/207 (47.83%), Query Frame = 2
Query: 10742 NLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ------DVYNMVKHFAHGIGD-----------STAKICSSASRVISEITMDPEYEEKR-QRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEG---QPPR 11299
N + SI HY +Q Q + +D LGNP G I + G++ L+ + V+ A+G+ + ++I + + I+ +T+D +Y+ KR ++L + G AQ G K L G G+ V T+ G K +G G F G KG+VGVVT+PV G++D A+ S R +A ++ E +PPR
Sbjct: 2977 NQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQ---GGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPR 3180 HSP 3 Score: 63.1586 bits (152), Expect = 6.887e-9 Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query: 5318 SARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKS 5791
S+R ++ I+ +AP++ +P ++ S + L+VDLG + N+F + N + +L+E KI L S +R++ SIL + +K N K + N+ + ++RNL+ N HT P I ++S+ +S Y + VL N E + + S
Sbjct: 1295 SSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQN--TSVVLEESKITL------SNLRVSRSILLENTVK---------NEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPS 1435 HSP 4 Score: 62.003 bits (149), Expect = 1.369e-8 Identity = 76/316 (24.05%), Postives = 146/316 (46.20%), Query Frame = 2
Query: 8156 TIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSC---QCTVLTTSMKQSTYFSVYLDSFPRCDPL-LIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQL-SNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNE-DQNKPIMALPASFLTFHWPRDDLDQLLTVQ 9085
+IE + + + + + + I+ P +L N+LP ++ + +SQ S + E LVR +C Q + T Q + LD P+ + L +I+ AK +S ++L V + + +G +V I S YW++N+T L Y + + +A ++ F+F +K+ F+ + S+A + ++++ LWS+K SL+ VG+ + V + + Y +G++I L TK+V Y++ N+SS LY CNE +N + +P WPR ++ + + ++
Sbjct: 2217 SIEEVQIVNSSETLRTYKLDINVPLALHNQLP--IKISYQSQIFSHDIKPGCLE-LVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYS---------ESFMDLGVHLAIK-NGQANVTIYSPYWMLNKTGYTLTYKAGDDCQV--------EHDAANTDAVLFSFLSKS-IFSKKKETISQAIVKAHLKINDSLWSDKFSLDTVGS------SGKVMCKKKPVTYEVGVKINLSSSGL--TKIVTFLPYYMILNQSSIVLY-----CNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMR 2497
BLAST of Vacuolar protein sorting 13 homolog D vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1) HSP 1 Score: 170.629 bits (431), Expect = 1.735e-41 Identity = 216/876 (24.66%), Postives = 385/876 (43.95%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWES---KTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLN----EMPKSDCIVNPISLHGIMKRNINPLPMNHQ-PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFA----ITEHTRHIFKG------AALRERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQSGWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQG-LEQDI--TELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDT--EIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-NPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTE----NIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLK--ISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALV----GNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLE 2539
++ L+ L+++ +G YV +K GL G+ LENL +K +D F+ P+ V+ G I ++TL+IP+ S+ ++ +Y++ PNS K EKE K+ L+ IE K+++ + E K S+ L +I++NLQI + N+H+RYED ST P++ FA G+ + S ++ D S K + T E K V + +++Y ++ K S + +K++++ ++ ++ ++ PIS +M ++ P N PR+SL L++EQ+ + ++ QY D +R + KYRP V + + WW F + E R + A R R R Y +E LL+K S E + E +L+L + R+ A E +K R L + GW+ +G+ ETS S S+ S L+K + + E+ L Q I +EL + D + + L+ L+ +K + ++ E ++++ ++ + R + + + + ++ V PD S +VS++ SK+ + + FETNPL + I I +EPL I++ + +I ++ + E I I K T+ ++ + +D R + +K I ILP T + +L+DLG + + + M P + ++DL FG L + KET YD++ I L + AL+ +W +L S+ +SKN ++ PF F +L KKCV+ + P I ++ + L + ISD+ L+
Sbjct: 1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDD-FDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIE---ENKLKRNDPKKETETKD--SFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTRE---FFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCR---LYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQA-EMKAKKMRKKLNQKTS-----SGGWFSGWFGKSDTETS------SSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPI------EYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPD---GSVPTLVSTQAQSKLMN--LLIVDFETNPLDKSANQKIN----IRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHL-----ADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSM-----------PGSGDTDL-----------IFGKDGKL----NYDVMKETA-----YDKFHIILSTIQALMIEQGDDWRKLRSQ---ESSKNH---ILRPFGFEFNL-KKCVITNDANLPKIFVKGCLPHLGVIISDRVLQ 794 HSP 2 Score: 69.3218 bits (168), Expect = 8.256e-11 Identity = 62/207 (29.95%), Postives = 99/207 (47.83%), Query Frame = 2
Query: 10742 NLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ------DVYNMVKHFAHGIGD-----------STAKICSSASRVISEITMDPEYEEKR-QRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEG---QPPR 11299
N + SI HY +Q Q + +D LGNP G I + G++ L+ + V+ A+G+ + ++I + + I+ +T+D +Y+ KR ++L + G AQ G K L G G+ V T+ G K +G G F G KG+VGVVT+PV G++D A+ S R +A ++ E +PPR
Sbjct: 2992 NQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQ---GGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPR 3195 HSP 3 Score: 63.1586 bits (152), Expect = 6.607e-9 Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query: 5318 SARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKS 5791
S+R ++ I+ +AP++ +P ++ S + L+VDLG + N+F + N + +L+E KI L S +R++ SIL + +K N K + N+ + ++RNL+ N HT P I ++S+ +S Y + VL N E + + S
Sbjct: 1295 SSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQN--TSVVLEESKITL------SNLRVSRSILLENTVK---------NEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPS 1435 HSP 4 Score: 62.003 bits (149), Expect = 1.428e-8 Identity = 76/316 (24.05%), Postives = 146/316 (46.20%), Query Frame = 2
Query: 8156 TIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSC---QCTVLTTSMKQSTYFSVYLDSFPRCDPL-LIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQL-SNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNE-DQNKPIMALPASFLTFHWPRDDLDQLLTVQ 9085
+IE + + + + + + I+ P +L N+LP ++ + +SQ S + E LVR +C Q + T Q + LD P+ + L +I+ AK +S ++L V + + +G +V I S YW++N+T L Y + + +A ++ F+F +K+ F+ + S+A + ++++ LWS+K SL+ VG+ + V + + Y +G++I L TK+V Y++ N+SS LY CNE +N + +P WPR ++ + + ++
Sbjct: 2232 SIEEVQIVNSSETLRTYKLDINVPLALHNQLP--IKISYQSQIFSHDIKPGCLE-LVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYS---------ESFMDLGVHLAIK-NGQANVTIYSPYWMLNKTGYTLTYKAGDDCQV--------EHDAANTDAVLFSFLSKS-IFSKKKETISQAIVKAHLKINDSLWSDKFSLDTVGS------SGKVMCKKKPVTYEVGVKINLSSSGL--TKIVTFLPYYMILNQSSIVLY-----CNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMR 2512
BLAST of Vacuolar protein sorting 13 homolog D vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1) HSP 1 Score: 167.548 bits (423), Expect = 1.714e-40 Identity = 213/879 (24.23%), Postives = 386/879 (43.91%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWES---KTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLN----EMPKSDCIVNPISLHGIMKRNINPLPMNHQ-PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFA----ITEHTRHIFKG------AALRERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQS---GWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQG-LEQDI--TELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDT--EIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-NPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTE----NIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLK--ISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALV----GNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLE 2539
++ L+ L+++ +G YV +K GL G+ LENL +K +D F+ P+ V+ G I ++TL+IP+ S+ ++ +Y++ PNS K EKE K+ L+ IE K+++ + E K S+ L +I++NLQI + N+H+RYED ST P++ FA G+ + S ++ D S K + T E K V + +++Y ++ K S + +K++++ ++ ++ ++ PIS +M ++ P N PR+SL L++EQ+ + ++ QY D +R + KYRP V + + WW F + E R + A R R R Y +E LL+K S E + E +L+L + R+ A + + + + Q++ GW+ +G+ ETS S S+ S L+K + + E+ L Q I +EL + D + + L+ L+ +K + ++ E ++++ ++ + R + + + + ++ V PD S +VS++ SK+ + + FETNPL + I I +EPL I++ + +I ++ + E I I K T+ ++ + +D R + +K I ILP T + +L+DLG + + + M P + ++DL FG L + KET YD++ I L + AL+ +W +L S+ +SKN ++ PF F +L KKCV+ + P I ++ + L + ISD+ L+
Sbjct: 1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDD-FDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIE---ENKLKRNDPKKETETKD--SFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTRE---FFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCR---LYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKVSKLKANKKAKKMRKKLNQKTSSGGWFSGWFGKSDTETS------SSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPI------EYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPD---GSVPTLVSTQAQSKLMN--LLIVDFETNPLDKSANQKIN----IRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHL-----ADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSM-----------PGSGDTDL-----------IFGKDGKL----NYDVMKETA-----YDKFHIILSTIQALMIEQGDDWRKLRSQ---ESSKNH---ILRPFGFEFNL-KKCVITNDANLPKIFVKGCLPHLGVIISDRVLQ 803 HSP 2 Score: 69.3218 bits (168), Expect = 7.986e-11 Identity = 62/207 (29.95%), Postives = 99/207 (47.83%), Query Frame = 2
Query: 10742 NLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ------DVYNMVKHFAHGIGD-----------STAKICSSASRVISEITMDPEYEEKR-QRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEG---QPPR 11299
N + SI HY +Q Q + +D LGNP G I + G++ L+ + V+ A+G+ + ++I + + I+ +T+D +Y+ KR ++L + G AQ G K L G G+ V T+ G K +G G F G KG+VGVVT+PV G++D A+ S R +A ++ E +PPR
Sbjct: 3001 NQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQ---GGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPR 3204 HSP 3 Score: 63.1586 bits (152), Expect = 6.777e-9 Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query: 5318 SARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKS 5791
S+R ++ I+ +AP++ +P ++ S + L+VDLG + N+F + N + +L+E KI L S +R++ SIL + +K N K + N+ + ++RNL+ N HT P I ++S+ +S Y + VL N E + + S
Sbjct: 1304 SSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQN--TSVVLEESKITL------SNLRVSRSILLENTVK---------NEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPS 1444 HSP 4 Score: 62.003 bits (149), Expect = 1.465e-8 Identity = 76/316 (24.05%), Postives = 146/316 (46.20%), Query Frame = 2
Query: 8156 TIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSC---QCTVLTTSMKQSTYFSVYLDSFPRCDPL-LIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQL-SNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNE-DQNKPIMALPASFLTFHWPRDDLDQLLTVQ 9085
+IE + + + + + + I+ P +L N+LP ++ + +SQ S + E LVR +C Q + T Q + LD P+ + L +I+ AK +S ++L V + + +G +V I S YW++N+T L Y + + +A ++ F+F +K+ F+ + S+A + ++++ LWS+K SL+ VG+ + V + + Y +G++I L TK+V Y++ N+SS LY CNE +N + +P WPR ++ + + ++
Sbjct: 2241 SIEEVQIVNSSETLRTYKLDINVPLALHNQLP--IKISYQSQIFSHDIKPGCLE-LVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYS---------ESFMDLGVHLAIK-NGQANVTIYSPYWMLNKTGYTLTYKAGDDCQV--------EHDAANTDAVLFSFLSKS-IFSKKKETISQAIVKAHLKINDSLWSDKFSLDTVGS------SGKVMCKKKPVTYEVGVKINLSSSGL--TKIVTFLPYYMILNQSSIVLY-----CNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMR 2521
BLAST of Vacuolar protein sorting 13 homolog D vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1) HSP 1 Score: 167.548 bits (423), Expect = 1.801e-40 Identity = 213/879 (24.23%), Postives = 386/879 (43.91%), Query Frame = 2
Query: 20 VMINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLFEWPILVKSGLIGRVTLRIPYTRFRSQAWAIMIEDIYIIAGPNSNWKLF-EKEFQNENMMKKLLLDSIEVKWRKKIRQQGDNDKVEGKIWLSYVASLGYRILRNLQIEVRNVHVRYEDNSTLPSQIFATGLLIDAFSTKSSDFEWES---KTELTTELDVLNKQVDLSGISIYLDTKCKIMSEVSVENIKSVLQEKLN----EMPKSDCIVNPISLHGIMKRNINPLPMNHQ-PRISLHLQMEQVEISLSQGQYKIAVALSDEFERFDRQKHHMKYRPRVSVMESPKEWWVFA----ITEHTRHIFKG------AALRERARNLVKYVQSYEDYLLNKD-SNLEAEFRNNLESDLELDCLLQLREVAMEHIGRKSRDLLQNAVNIPAPQQS---GWWGYLWGRRSKETSPEILDQSQYNSETSQILEKLLPRDSNTSEQG-LEQDI--TELLNEVDFEAVNFFTRDKIFLRGEFSLEGIKLKLLKDT--EIPILEVNAKNLIVGFESRPQYSSWCIRIGLRSLFVRDL-NPDYKNDSFRYVVSSREHSKVPGGEIFALAFETNPLPSTCPPNIKRILAISSEPLTIVHCPLLIGNIQEYFSRSTE----NIKAAAEKHIRNAKARTSESIKKILEKGSSDIRWWVSLK--ISAPKIILPNFRLKTHEPSCVLLDLGRLQVSNCSDQMIDRSIKVTAPEPMNYESDLESEDFVTPCSSPFGSLENLSMATDCQASKETKPVTNIYDEYSIKLENVMALV----GNWNELESELSKNTSKNPKLLLMEPFNFILDLKKKCVMEKSSQSPNIIMESNISRLLISISDQKLE 2539
++ L+ L+++ +G YV +K GL G+ LENL +K +D F+ P+ V+ G I ++TL+IP+ S+ ++ +Y++ PNS K EKE K+ L+ IE K+++ + E K S+ L +I++NLQI + N+H+RYED ST P++ FA G+ + S ++ D S K + T E K V + +++Y ++ K S + +K++++ ++ ++ ++ PIS +M ++ P N PR+SL L++EQ+ + ++ QY D +R + KYRP V + + WW F + E R + A R R R Y +E LL+K S E + E +L+L + R+ A + + + + Q++ GW+ +G+ ETS S S+ S L+K + + E+ L Q I +EL + D + + L+ L+ +K + ++ E ++++ ++ + R + + + + ++ V PD S +VS++ SK+ + + FETNPL + I I +EPL I++ + +I ++ + E I I K T+ ++ + +D R + +K I ILP T + +L+DLG + + + M P + ++DL FG L + KET YD++ I L + AL+ +W +L S+ +SKN ++ PF F +L KKCV+ + P I ++ + L + ISD+ L+
Sbjct: 1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDD-FDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIE---ENKLKRNDPKKETETKD--SFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTRE---FFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCR---LYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKVSKLKANKKAKKMRKKLNQKTSSGGWFSGWFGKSDTETS------SSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPI------EYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPD---GSVPTLVSTQAQSKLMN--LLIVDFETNPLDKSANQKIN----IRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHL-----ADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSM-----------PGSGDTDL-----------IFGKDGKL----NYDVMKETA-----YDKFHIILSTIQALMIEQGDDWRKLRSQ---ESSKNH---ILRPFGFEFNL-KKCVITNDANLPKIFVKGCLPHLGVIISDRVLQ 803 HSP 2 Score: 69.3218 bits (168), Expect = 8.257e-11 Identity = 62/207 (29.95%), Postives = 99/207 (47.83%), Query Frame = 2
Query: 10742 NLIAQSIAYHYRNQLRSQAMKIFGSVDFLGNPIGFISDITTGMKRLYSDQ------DVYNMVKHFAHGIGD-----------STAKICSSASRVISEITMDPEYEEKR-QRLFENARISGSNTAQVKAGFKSLAYGIWGGIKSVPTQLDKGYKDEGPSGLFIGALKGIVGVVTKPVGGMLDLATGSATSFREIARSSKEG---QPPR 11299
N + SI HY +Q Q + +D LGNP G I + G++ L+ + V+ A+G+ + ++I + + I+ +T+D +Y+ KR ++L + G AQ G K L G G+ V T+ G K +G G F G KG+VGVVT+PV G++D A+ S R +A ++ E +PPR
Sbjct: 2994 NQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQ---GGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPR 3197 HSP 3 Score: 63.1586 bits (152), Expect = 6.948e-9 Identity = 45/158 (28.48%), Postives = 81/158 (51.27%), Query Frame = 2
Query: 5318 SARIRMKISCQAPLLLLPVSAFSDHMLIVDLGKFSLVNEFKFENNDRSRCLLDEMKIDLHEMDVYSAIRITDSILKDGDLKFSYLNIRPINPKPLSEKCNLNLTVSRNLNCNISHTSPDWQIYGILSSVIFSVSVEQYRTIRGVLAHNLGENTDHLKS 5791
S+R ++ I+ +AP++ +P ++ S + L+VDLG + N+F + N + +L+E KI L S +R++ SIL + +K N K + N+ + ++RNL+ N HT P I ++S+ +S Y + VL N E + + S
Sbjct: 1297 SSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQN--TSVVLEESKITL------SNLRVSRSILLENTVK---------NEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPS 1437 HSP 4 Score: 62.003 bits (149), Expect = 1.302e-8 Identity = 76/316 (24.05%), Postives = 146/316 (46.20%), Query Frame = 2
Query: 8156 TIERLNFPLAPKMVPGHLITISAPFSLENRLPCDVRFAMKSQTPSSSMPAITNEALVRPSC---QCTVLTTSMKQSTYFSVYLDSFPRCDPL-LIHGDAKLSHSVLIRLYDDQNRLLELEVKVDSRPSGPVSVVISSFYWIVNRTSLPLVYAQSGETETLAAGHVPENEEACSSMPFPFNFSNKNDSFTFRVRLGSKAQL-SNMENFKPLWSEKVSLEKVGTTTLQLRAASVYQNRPNLLYNIGLEIRLGRGKHINTKMVILTARYLLDNRSSFPLYFIQYNCNE-DQNKPIMALPASFLTFHWPRDDLDQLLTVQ 9085
+IE + + + + + + I+ P +L N+LP ++ + +SQ S + E LVR +C Q + T Q + LD P+ + L +I+ AK +S ++L V + + +G +V I S YW++N+T L Y + + +A ++ F+F +K+ F+ + S+A + ++++ LWS+K SL+ VG+ + V + + Y +G++I L TK+V Y++ N+SS LY CNE +N + +P WPR ++ + + ++
Sbjct: 2234 SIEEVQIVNSSETLRTYKLDINVPLALHNQLP--IKISYQSQIFSHDIKPGCLE-LVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYS---------ESFMDLGVHLAIK-NGQANVTIYSPYWMLNKTGYTLTYKAGDDCQV--------EHDAANTDAVLFSFLSKS-IFSKKKETISQAIVKAHLKINDSLWSDKFSLDTVGS------SGKVMCKKKPVTYEVGVKINLSSSGL--TKIVTFLPYYMILNQSSIVLY-----CNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMR 2514 The following BLAST results are available for this feature:
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Vacuolar protein sorting 13 homolog D vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016802 ID=SMED30016802|Name=Vacuolar protein sorting 13 homolog D|organism=Schmidtea mediterranea sexual|type=transcript|length=11864bpback to top protein sequence of SMED30016802-orf-1 >SMED30016802-orf-1 ID=SMED30016802-orf-1|Name=SMED30016802-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=3933bp MINQLITYLLDRVIGKYVENFSTQNVKTGLFKGDIRLENLPLKNTVLEDLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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