SMED30016771

Overview
NameSMED30016771
Smed IDSMED30016771
Length (bp)560
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016771 (SMED30016771) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016771 (SMED30016771) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016771

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30016771 dd_Smed_v4_46708_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30016771 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851])

HSP 1 Score: 62.003 bits (149), Expect = 5.996e-11
Identity = 50/182 (27.47%), Postives = 86/182 (47.25%), Query Frame = -3 
Query:    1 VHW--QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVT-HLEKLIRDMERRFADLVQLNIPD-WVLS--LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVS--STWLHFHDRNPKLFVIAQPLLLKFPTSYIA 522
            V+W  ++ Y+SD+F  FN+ N  +QG+  T       V    +K   +++ I+   +  F  L   + E     D   L   ++ HL  L+   E  F       I + W+ +  L + D +N    +Q+ L+K+  DE L++  +N  S  S W+   +  P+L  IA  LLL FP++Y+ 
Sbjct:  945 VNWTARLAYLSDIFSIFNDLNASMQGKNATYFSMADKVEGQKQKLEAWKNRISTDCYDMFHNLTTIINEVGNDLDIAHLRKVISEHLTNLLECFEFYFPSKEDPRIGNLWIQNPFLSSKDNLNLTVTLQDKLLKLATDEGLKISFENTASLPSFWIKAKNDYPELAEIALKLLLLFPSTYLC 1126
[Close]
BLAST of SMED30016771 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851])

HSP 1 Score: 62.003 bits (149), Expect = 6.676e-11
Identity = 50/182 (27.47%), Postives = 86/182 (47.25%), Query Frame = -3 
Query:    1 VHW--QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVT-HLEKLIRDMERRFADLVQLNIPD-WVLS--LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVS--STWLHFHDRNPKLFVIAQPLLLKFPTSYIA 522
            V+W  ++ Y+SD+F  FN+ N  +QG+  T       V    +K   +++ I+   +  F  L   + E     D   L   ++ HL  L+   E  F       I + W+ +  L + D +N    +Q+ L+K+  DE L++  +N  S  S W+   +  P+L  IA  LLL FP++Y+ 
Sbjct: 1096 VNWTARLAYLSDIFSIFNDLNASMQGKNATYFSMADKVEGQKQKLEAWKNRISTDCYDMFHNLTTIINEVGNDLDIAHLRKVISEHLTNLLECFEFYFPSKEDPRIGNLWIQNPFLSSKDNLNLTVTLQDKLLKLATDEGLKISFENTASLPSFWIKAKNDYPELAEIALKLLLLFPSTYLC 1277
[Close]
BLAST of SMED30016771 vs. Ensembl Human
Match: ZBED5 (zinc finger BED-type containing 5 [Source:HGNC Symbol;Acc:HGNC:30803])

HSP 1 Score: 50.0618 bits (118), Expect = 7.198e-7
Identity = 27/84 (32.14%), Postives = 44/84 (52.38%), Query Frame = -3 
Query:  262 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKL 513
            ++ Y++D+F + NE NL +QG+ VT+      + SL++K   + S++    F  FP L  F+ E +   D D     V HL  L
Sbjct:  473 RLAYLADIFTKLNEVNLSMQGKNVTVFTVFDKMSSLLRKLEFWASSVEEENFDCFPTLSDFLTEINSTVDKDICSAIVQHLRGL 556
[Close]
BLAST of SMED30016771 vs. Ensembl Human
Match: ZBED5 (zinc finger BED-type containing 5 [Source:HGNC Symbol;Acc:HGNC:30803])

HSP 1 Score: 50.0618 bits (118), Expect = 7.198e-7
Identity = 27/84 (32.14%), Postives = 44/84 (52.38%), Query Frame = -3 
Query:  262 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKL 513
            ++ Y++D+F + NE NL +QG+ VT+      + SL++K   + S++    F  FP L  F+ E +   D D     V HL  L
Sbjct:  473 RLAYLADIFTKLNEVNLSMQGKNVTVFTVFDKMSSLLRKLEFWASSVEEENFDCFPTLSDFLTEINSTVDKDICSAIVQHLRGL 556
[Close]
BLAST of SMED30016771 vs. Ensembl Mouse
Match: Zbed5 (zinc finger, BED type containing 5 [Source:MGI Symbol;Acc:MGI:1919220])

HSP 1 Score: 53.1434 bits (126), Expect = 4.795e-8
Identity = 46/178 (25.84%), Postives = 76/178 (42.70%), Query Frame = -3 
Query:    1 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESS-EKSDADELMIYVT-HLEKLIRDMERRFADLVQLNIPDWVLSLE----NLDLMNYPADIQEALIKVHNDEELRVEMKN-DVSSTWLHFHDRNPKLFVIAQPLLLKFPTSYIA 513
            ++ Y+ D+F   NE NL LQG   T +D    + S   K  L++  +    F   P L  F  E   E+     ++I V  HL+ L  ++   F      N+P+   +L     ++   + P   QE   ++ N +  R +     V+  W+      P L  +   LLL FPT+Y+ 
Sbjct:  536 KVAYLVDIFTILNELNLSLQGPNSTCLDLSEKIHSFQMKLQLWQKKLDENKFYMLPTLSAFFEEHDIEQHKRITVVISVKEHLDMLASEISWYFP-----NLPEIPFALARSPFSVKAEDVPETAQEDFTRLTNSDAARADFSTMPVTQFWVKCLQSYPVLSEMVLRLLLPFPTTYLC 708
[Close]
BLAST of SMED30016771 vs. UniProt/SwissProt
Match: sp|Q6R2W3|SCND3_HUMAN (SCAN domain-containing protein 3 OS=Homo sapiens OX=9606 GN=ZBED9 PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 3.206e-10
Identity = 50/182 (27.47%), Postives = 86/182 (47.25%), Query Frame = -3 
Query:    1 VHW--QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVT-HLEKLIRDMERRFADLVQLNIPD-WVLS--LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVS--STWLHFHDRNPKLFVIAQPLLLKFPTSYIA 522
            V+W  ++ Y+SD+F  FN+ N  +QG+  T       V    +K   +++ I+   +  F  L   + E     D   L   ++ HL  L+   E  F       I + W+ +  L + D +N    +Q+ L+K+  DE L++  +N  S  S W+   +  P+L  IA  LLL FP++Y+ 
Sbjct: 1096 VNWTARLAYLSDIFSIFNDLNASMQGKNATYFSMADKVEGQKQKLEAWKNRISTDCYDMFHNLTTIINEVGNDLDIAHLRKVISEHLTNLLECFEFYFPSKEDPRIGNLWIQNPFLSSKDNLNLTVTLQDKLLKLATDEGLKISFENTASLPSFWIKAKNDYPELAEIALKLLLLFPSTYLC 1277
[Close]
BLAST of SMED30016771 vs. UniProt/SwissProt
Match: sp|Q49AG3|ZBED5_HUMAN (Zinc finger BED domain-containing protein 5 OS=Homo sapiens OX=9606 GN=ZBED5 PE=2 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 3.457e-6
Identity = 27/84 (32.14%), Postives = 44/84 (52.38%), Query Frame = -3 
Query:  262 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKL 513
            ++ Y++D+F + NE NL +QG+ VT+      + SL++K   + S++    F  FP L  F+ E +   D D     V HL  L
Sbjct:  473 RLAYLADIFTKLNEVNLSMQGKNVTVFTVFDKMSSLLRKLEFWASSVEEENFDCFPTLSDFLTEINSTVDKDICSAIVQHLRGL 556
[Close]
BLAST of SMED30016771 vs. UniProt/SwissProt
Match: sp|A4Z944|ZBED5_CANLF (Zinc finger BED domain-containing protein 5 OS=Canis lupus familiaris OX=9615 GN=ZBED5 PE=4 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 3.490e-6
Identity = 28/92 (30.43%), Postives = 47/92 (51.09%), Query Frame = -3 
Query:  238 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRF 513
            ++ Y++D+F + NE NL +QG+ VT+      + SL++K   + S++    F  FP L  F+ E +   D D     V HL  L   + + F
Sbjct:  474 RLAYLADIFTKLNEVNLSMQGKNVTVFTVFDKMSSLLRKLEFWASSVEEENFDCFPTLSDFLTEINSTVDKDICSAIVQHLRGLRSTLLKYF 565
[Close]
BLAST of SMED30016771 vs. UniProt/SwissProt
Match: sp|A4Z943|ZBED5_BOVIN (Zinc finger BED domain-containing protein 5 OS=Bos taurus OX=9913 GN=ZBED5 PE=4 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 3.523e-6
Identity = 44/176 (25.00%), Postives = 81/176 (46.02%), Query Frame = -3 
Query:    1 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVLSLENLDLMNYPADI----QEALIKVHNDEELRVEMKN-DVSSTWLHFHDRNPKLFVIAQPLLLKFPTSYIA 513
            ++ Y++D+F + NE NL +QG+ VT+      + SL++K   + S++    F  FP L  F+ E +   D +     V HL  L   + + F   V  +I  WV +   + +   PA +     E+LI + +D +++       ++  W       P +   A  +LL F T ++ 
Sbjct:  474 RLAYLADIFTKLNEVNLSMQGKNVTVFTVFDKMSSLLRKLEFWASSVEEENFDCFPTLSDFLTEINSTVDKNICSAIVQHLRGLRSTLLKYFP--VTNDIHAWVRNPFTVTVK--PASLVARDYESLIDLTSDSQVKQSFSELSLNDFWSSLIQEYPSVARRAVRVLLPFATMHLC 645
[Close]
BLAST of SMED30016771 vs. TrEMBL
Match: A0A087UJJ1 (SCAN domain-containing protein 3 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_22956 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 9.517e-30
Identity = 65/173 (37.57%), Postives = 101/173 (58.38%), Query Frame = -3 
Query:    1 ICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVLS-LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVSSTWLH--FHDRNPKLFVIAQPLLLKFPTSYIA 510
            I Y++D++  FN+ NLQLQG+ + LI  K  + + + K  L++ NI RR F  FP L      S+     D+L++Y  HLE L  D + RF D++ ++IPDWVL    N++    P+ ++E LI++  +EEL+++ KN     WL        P+L+ I Q  LL FP+SY+A
Sbjct:  125 IAYLADLYKLFNDVNLQLQGEDLNLIKTKNSIAAFVSKLLLYKRNIGRREFHTFPCL------SAVSFKHDDLLVYCQHLENLYNDFKERFEDILNMDIPDWVLDPFSNVNTAG-PSQLEEELIELTTNEELKIKFKNGYQEFWLQKSISQLYPRLWSIVQRFLLAFPSSYLA 290
[Close]
BLAST of SMED30016771 vs. TrEMBL
Match: A0A087U6F5 (SCAN domain-containing protein 3 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_19689 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.435e-28
Identity = 65/173 (37.57%), Postives = 101/173 (58.38%), Query Frame = -3 
Query:    1 ICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVLS-LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVSSTWLH--FHDRNPKLFVIAQPLLLKFPTSYIA 510
            I Y++D++  FN+ NLQLQG+ + LI  K  + + + K  L++ NI RR F  FP L      S+     D+L++Y  HLE L  D + RF D++ ++IPDWVL    N++    P+ ++E LI++  +EEL+++ KN     WL        P+L+ I Q  LL FP+SY+A
Sbjct:  310 IAYLADLYKLFNDVNLQLQGEDLNLIKTKNSIAAFVSKLLLYKRNIGRREFHTFPCL------SAVSFKHDDLLVYCQHLENLYNDFKERFEDILNMDIPDWVLDPFSNVNTAG-PSQLEEELIELTTNEELKIKFKNGYQEFWLQKSISQLYPRLWSIVQRFLLAFPSSYLA 475
[Close]
BLAST of SMED30016771 vs. TrEMBL
Match: A0A087T171 (SCAN domain-containing protein 3 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_26257 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.546e-28
Identity = 65/173 (37.57%), Postives = 101/173 (58.38%), Query Frame = -3 
Query:    1 ICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVLS-LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVSSTWLH--FHDRNPKLFVIAQPLLLKFPTSYIA 510
            I Y++D++  FN+ NLQLQG+ + LI  K  + + + K  L++ NI RR F  FP L      S+     D+L++Y  HLE L  D + RF D++ ++IPDWVL    N++    P+ ++E LI++  +EEL+++ KN     WL        P+L+ I Q  LL FP+SY+A
Sbjct:  374 IAYLADLYKLFNDVNLQLQGEDLNLIKTKNSIAAFVSKLLLYKRNIGRREFHTFPCL------SAVSFKHDDLLVYCQHLENLYNDFKERFEDILNMDIPDWVLDPFSNVNTAG-PSQLEEELIELTTNEELKIKFKNGYQEFWLQKSISQLYPRLWSIVQRFLLAFPSSYLA 539
[Close]
BLAST of SMED30016771 vs. TrEMBL
Match: A0A087T1H3 (SCAN domain-containing protein 3 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_23972 PE=4 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.991e-28
Identity = 65/173 (37.57%), Postives = 101/173 (58.38%), Query Frame = -3 
Query:    1 ICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVLS-LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVSSTWLH--FHDRNPKLFVIAQPLLLKFPTSYIA 510
            I Y++D++  FN+ NLQLQG+ + LI  K  + + + K  L++ NI RR F  FP L      S+     D+L++Y  HLE L  D + RF D++ ++IPDWVL    N++    P+ ++E LI++  +EEL+++ KN     WL        P+L+ I Q  LL FP+SY+A
Sbjct:  374 IAYLADLYKLFNDVNLQLQGEDLNLIKTKNSIAAFVSKLLLYKRNIGRREFHTFPCL------SAVSFKHDDLLVYCQHLENLYNDFKERFEDILNMDIPDWVLDPFSNVNTAG-PSQLEEELIELTTNEELKIKFKNGYQEFWLQKSISQLYPRLWSIVQRFLLAFPSSYLA 539
[Close]
BLAST of SMED30016771 vs. TrEMBL
Match: A0A087T9G6 (SCAN domain-containing protein 3 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_16128 PE=4 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 2.387e-28
Identity = 65/173 (37.57%), Postives = 101/173 (58.38%), Query Frame = -3 
Query:    1 ICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVLS-LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVSSTWLH--FHDRNPKLFVIAQPLLLKFPTSYIA 510
            I Y++D++  FN+ NLQLQG+ + LI  K  + + + K  L++ NI RR F  FP L      S+     D+L++Y  HLE L  D + RF D++ ++IPDWVL    N++    P+ ++E LI++  +EEL+++ KN     WL        P+L+ I Q  LL FP+SY+A
Sbjct:  331 IAYLADLYKLFNDVNLQLQGEDLNLIKTKNSIAAFVSKLLLYKRNIGRREFHTFPCL------SAVSFKHDDLLVYCQHLENLYNDFKERFEDILNMDIPDWVLDPFSNVNTAG-PSQLEEELIELTTNEELKIKFKNGYQEFWLQKSISQLYPRLWSIVQRFLLAFPSSYLA 496
[Close]
BLAST of SMED30016771 vs. Ensembl Cavefish
Match: ENSAMXT00000057314.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:4224188:4227217:-1 gene:ENSAMXG00000029268.1 transcript:ENSAMXT00000057314.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 2.710e-7
Identity = 30/104 (28.85%), Postives = 56/104 (53.85%), Query Frame = -3 
Query:  199 YMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMI-YVTHLEKLIRDMERRFADLVQLNIPD-WVL 504
            Y++D+F  +NE+N ++QG   T+I+ K  V + ++K    R+ + +     FP+L     + S     D L   +  H+++L  +M  RF+D+ +L   D WV+
Sbjct:  343 YLADIFSLYNEANKRMQGADGTVIECKETVDAFVRKVEFRRNKLKKGDLQHFPLL----LQQSGGVLPDALTTQFACHMDRLQEEMHSRFSDIHELVSKDAWVM 442
[Close]
BLAST of SMED30016771 vs. Ensembl Medaka
Match: ENSORLT00000031208.1 (pep primary_assembly:ASM223467v1:14:26692610:26696672:-1 gene:ENSORLG00000026199.1 transcript:ENSORLT00000031208.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.707 bits (169), Expect = 6.192e-14
Identity = 47/176 (26.70%), Postives = 90/176 (51.14%), Query Frame = -3 
Query:    1 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIP-DWVLS---LENLDLMNYPADIQEALIKVHNDEELRVEM-KNDVSSTWLHFHDRNPKLFVIAQPLLLKFPTSYIA 513
            ++CY++D+F + NE N+ LQG+  ++++    V   +KK  +++    +  F  FP LD F+  + + + A   ++ V HL  LI      F D+ + ++  DWV +   L   +    P   QE L++V +D  L+++   + ++  WL     +P+L   A   LL F ++Y+ 
Sbjct:  382 KLCYLADIFRKLNELNMSLQGKDTSILNLYDKVGGFLKKVEMWKRACDQEDFTCFPQLDVFL-SNEDVATAPVKLVIVGHLANLISGFHSYFPDMDEKSVQLDWVRNPFLLSEANRSKLPVTHQEKLMEVSSDRGLQMKFGASTLTQFWLCVRQEHPELGQKALEQLLPFASTYLC 556
[Close]
BLAST of SMED30016771 vs. Ensembl Medaka
Match: ENSORLT00000035574.1 (pep primary_assembly:ASM223467v1:10:5595259:5596581:-1 gene:ENSORLG00000022539.1 transcript:ENSORLT00000035574.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.3218 bits (168), Expect = 8.957e-14
Identity = 47/176 (26.70%), Postives = 90/176 (51.14%), Query Frame = -3 
Query:    1 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIP-DWVLS---LENLDLMNYPADIQEALIKVHNDEELRVEM-KNDVSSTWLHFHDRNPKLFVIAQPLLLKFPTSYIA 513
            ++CY++D+F + NE N+ LQG+  ++++    V   +KK  +++    +  F  FP LD F+  + + + A   ++ V HL  LI      F D+ + ++  DWV +   L   +    P   QE L++V +D  L+++   + ++  WL     +P+L   A   LL F ++Y+ 
Sbjct:  218 KLCYLADIFRKLNELNMSLQGKDTSILNLYDKVGGFLKKAEMWKRACDQEDFTCFPQLDVFL-SNEDVATAPVKLVIVGHLANLISGFHSYFPDMDEKSVQLDWVRNPFLLSEANRSKLPVTHQEKLMEVSSDRGLQMKFGASTLTQFWLCVRQEHPELGQKALEQLLPFASTYLC 392
[Close]
BLAST of SMED30016771 vs. Ensembl Medaka
Match: ENSORLT00000030788.1 (pep primary_assembly:ASM223467v1:17:2264135:2274164:1 gene:ENSORLG00000023031.1 transcript:ENSORLT00000030788.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 67.3958 bits (163), Expect = 4.689e-13
Identity = 47/176 (26.70%), Postives = 89/176 (50.57%), Query Frame = -3 
Query:    1 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIP-DWVLS---LENLDLMNYPADIQEALIKVHNDEELRVEM-KNDVSSTWLHFHDRNPKLFVIAQPLLLKFPTSYIA 513
            ++CY++D+F + NE N+ LQG+  ++++    V   +KK  +++    +  F  FP LD F+  + + + A   ++ V HL  LI      F D  + ++  DWV +   L   +    P   QE L++V +D  L+++   + ++  WL     +P+L   A   LL F ++Y+ 
Sbjct:  359 KLCYLADIFRKLNELNMSLQGKNTSILNLYDKVGGFLKKAEMWKRACDQEDFTCFPQLDVFL-SNEDVATAPVKLVIVGHLANLISGFHSYFPDTDEKSVQLDWVRNPFLLSEANRSKLPVTHQEKLMEVSSDRGLQMKFGASTLTQFWLCERQEHPELGQKALEQLLPFASTYLC 533
[Close]
BLAST of SMED30016771 vs. Ensembl Medaka
Match: ENSORLT00000028946.1 (pep primary_assembly:ASM223467v1:20:802231:804233:-1 gene:ENSORLG00000029187.1 transcript:ENSORLT00000028946.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 66.2402 bits (160), Expect = 1.014e-12
Identity = 50/178 (28.09%), Postives = 90/178 (50.56%), Query Frame = -3 
Query:    1 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIP-DWVLS---LENLDLMNYPADIQEALIKVHNDEELRVEMKNDVSST---WLHFHDRNPKLFVIAQPLLLKFPTSYIA 513
            ++CY++D+F + +E N+ LQG+  ++++    V   +KK  +++    +  F  FP LD F+  + + + A   ++ V HL  LI      F D+ + ++  DWV +   L   +    P   QE L++V +D  L  +MK  VS+    WL     +P+L   A   LL F ++Y+ 
Sbjct:  241 KLCYLADIFRKLSELNMSLQGKDTSILNLYDKVGGFLKKAEMWKRACDQEDFTCFPQLDVFL-SNEDVATAPVKLVIVGHLANLISGFHSYFPDMDEKSVQLDWVRNPFLLSEANRSKLPVTHQEKLMEVASDRGL--QMKFGVSTLTQFWLCERQEHPELGQKALEQLLPFASTYLC 415
[Close]
BLAST of SMED30016771 vs. Ensembl Medaka
Match: ENSORLT00000040762.1 (pep primary_assembly:ASM223467v1:1:8553592:8557739:1 gene:ENSORLG00000022190.1 transcript:ENSORLT00000040762.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 64.3142 bits (155), Expect = 4.760e-12
Identity = 47/177 (26.55%), Postives = 90/177 (50.85%), Query Frame = -3 
Query:    1 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHL-EKLIRDMERRFADLVQLNIP-DWVLS---LENLDLMNYPADIQEALIKVHNDEELRVEM-KNDVSSTWLHFHDRNPKLFVIAQPLLLKFPTSYIA 513
            ++CY++D+F + NE N+ LQG+  ++++    V   +KK  +++    +  F  FP LD F+  + + + A   ++ V HL   LI      F D+ + ++  DWV +   L   +    P   QE L++V +D  L+++   + ++  WL     +P+L   A   LL F ++Y+ 
Sbjct:  432 KLCYLADIFRKLNELNMSLQGKDTSILNLYDKVGGFLKKAEMWKRACDQEDFTCFPQLDVFL-SNEDVATAPVKLVIVGHLAANLISGFHSYFPDMDEKSVQLDWVRNPFLLSEANRSKLPVTHQEKLMEVSSDRGLQMKFGASTLTQFWLCVRQEHPELGQKALEQLLPFASTYLC 607
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BLAST of SMED30016771 vs. Planmine SMEST
Match: SMESG000000520.1 (SMESG000000520.1)

HSP 1 Score: 138.272 bits (347), Expect = 4.930e-42
Identity = 71/95 (74.74%), Postives = 77/95 (81.05%), Query Frame = -3 
Query:  274 SDGLKEKLAIPSVHWQICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTH 558
            SDG+KEK  IPSVH  ICYMSDVF+RFNESNLQLQGQKVTLIDGKRVV SL+K FTLF+SN   R F QFPVL GFV E S +   DEL+IYVTH
Sbjct:   27 SDGIKEKFVIPSVHRLICYMSDVFNRFNESNLQLQGQKVTLIDGKRVVSSLIKTFTLFKSN---RSFQQFPVLVGFVPEGSVEPSDDELIIYVTH 118
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BLAST of SMED30016771 vs. Planmine SMEST
Match: SMESG000068927.1 (SMESG000068927.1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.676e-22
Identity = 53/145 (36.55%), Postives = 81/145 (55.86%), Query Frame = -3 
Query:   79 ICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVL-SLENLDLMNYPADIQEALIKVHNDEELRVEMKNDVSSTWL 510
            I Y++D++   N  NLQLQ   + LI  K VV + + K  L +++I RR F  FP L       S   + D+L+ Y  HLE L  D   RF D+++L IP+WVL S  N+ +   P  ++E LI++  +EE++++ KN     WL
Sbjct:   12 IAYLTDLYTLLNHMNLQLQSDDLNLIKTKNVVAAFVAKLLLHKNSIGRREFHNFPNL-------SVSCNNDDLLAYCQHLENLHSDFIERFQDILKLEIPNWVLDSSLNVKIAISP-QLEEELIELTTNEEIKIKFKNGYQQFWL 148
[Close]
BLAST of SMED30016771 vs. Planmine SMEST
Match: SMESG000024660.1 (SMESG000024660.1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.198e-16
Identity = 49/157 (31.21%), Postives = 77/157 (49.04%), Query Frame = -3 
Query:    4 NLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVLSLENLDLMNYPADIQEALIKVHNDEELRVEMKNDVSSTWL--HFHDRNPKLFVIAQPLLLKFPTSYI 468
            NL LQG  + LI  K ++ + +    + + NI R  F QF  L      S      +++  YV HL  L  D E RF D++ + IP W+++    D+      IQE L ++  + EL+V+ K+     WL  +     P L  IA+  L+ FP+SY+
Sbjct:    3 NLHLQGNSLNLIKTKSILSAFLAIVKIIKQNIGRGKFSQFSNL------SQTNCQENDVSTYVQHLNALYSDFETRFEDVLTMVIPPWIINPYG-DIEETNVIIQEELTEISTNGELKVKFKHGYQHFWLKKNIPITYPVLRNIARKFLISFPSSYL 152
[Close]
BLAST of SMED30016771 vs. Planmine SMEST
Match: SMESG000029634.1 (SMESG000029634.1)

HSP 1 Score: 65.855 bits (159), Expect = 6.354e-13
Identity = 31/105 (29.52%), Postives = 56/105 (53.33%), Query Frame = -3 
Query:  199 QICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVL 513
             I Y++D++  FN +  +LQ + + L+D K ++ S      LF S + R+ F  F  L        + + + ++++Y THL+ LI D   RF D+  L +P W++
Sbjct:  184 HIFYLADIYGLFNATQNKLQRRNINLVDAKTIIFSFRSNINLFISTLNRKEFRYFENLMQLSVIEKDCNSSQDIILYTTHLKNLIEDFNVRFRDMENLCVPAWII 288
[Close]
BLAST of SMED30016771 vs. Planmine SMEST
Match: SMESG000066855.1 (SMESG000066855.1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.393e-12
Identity = 32/94 (34.04%), Postives = 51/94 (54.26%), Query Frame = -3 
Query:  199 FNESNLQLQGQKVTLIDGKRVVLSLMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLIRDMERRFADLVQLNIPDWVL 480
            FN+    LQ  ++ LI  K V+ + + K  L + N  R  F QFP L G       K+  ++++ Y  HL+ L  D  +RF ++ ++NIPDW+L
Sbjct:  205 FNKGQFILQSYELNLIKTKSVISAFVIKLLLIKRNFGRGEFCQFPNLSG------AKTKDEDILSYCEHLDALYLDFNQRFDNIFKMNIPDWIL 292
The following BLAST results are available for this feature:
BLAST of SMED30016771 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ZBED95.996e-1127.47zinc finger BED-type containing 9 [Source:HGNC Sym... [more]
ZBED96.676e-1127.47zinc finger BED-type containing 9 [Source:HGNC Sym... [more]
ZBED57.198e-732.14zinc finger BED-type containing 5 [Source:HGNC Sym... [more]
ZBED57.198e-732.14zinc finger BED-type containing 5 [Source:HGNC Sym... [more]
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BLAST of SMED30016771 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016771 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016771 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016771 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016771 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Zbed54.795e-825.84zinc finger, BED type containing 5 [Source:MGI Sym... [more]
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BLAST of SMED30016771 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q6R2W3|SCND3_HUMAN3.206e-1027.47SCAN domain-containing protein 3 OS=Homo sapiens O... [more]
sp|Q49AG3|ZBED5_HUMAN3.457e-632.14Zinc finger BED domain-containing protein 5 OS=Hom... [more]
sp|A4Z944|ZBED5_CANLF3.490e-630.43Zinc finger BED domain-containing protein 5 OS=Can... [more]
sp|A4Z943|ZBED5_BOVIN3.523e-625.00Zinc finger BED domain-containing protein 5 OS=Bos... [more]
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BLAST of SMED30016771 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A087UJJ19.517e-3037.57SCAN domain-containing protein 3 (Fragment) OS=Ste... [more]
A0A087U6F51.435e-2837.57SCAN domain-containing protein 3 (Fragment) OS=Ste... [more]
A0A087T1711.546e-2837.57SCAN domain-containing protein 3 (Fragment) OS=Ste... [more]
A0A087T1H31.991e-2837.57SCAN domain-containing protein 3 (Fragment) OS=Ste... [more]
A0A087T9G62.387e-2837.57SCAN domain-containing protein 3 (Fragment) OS=Ste... [more]
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BLAST of SMED30016771 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000057314.12.710e-728.85pep primary_assembly:Astyanax_mexicanus-2.0:1:4224... [more]
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BLAST of SMED30016771 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016771 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016771 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016771 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000031208.16.192e-1426.70pep primary_assembly:ASM223467v1:14:26692610:26696... [more]
ENSORLT00000035574.18.957e-1426.70pep primary_assembly:ASM223467v1:10:5595259:559658... [more]
ENSORLT00000030788.14.689e-1326.70pep primary_assembly:ASM223467v1:17:2264135:227416... [more]
ENSORLT00000028946.11.014e-1228.09pep primary_assembly:ASM223467v1:20:802231:804233:... [more]
ENSORLT00000040762.14.760e-1226.55pep primary_assembly:ASM223467v1:1:8553592:8557739... [more]
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BLAST of SMED30016771 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000000520.14.930e-4274.74SMESG000000520.1[more]
SMESG000068927.11.676e-2236.55SMESG000068927.1[more]
SMESG000024660.11.198e-1631.21SMESG000024660.1[more]
SMESG000029634.16.354e-1329.52SMESG000029634.1[more]
SMESG000066855.11.393e-1234.04SMESG000066855.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016771 ID=SMED30016771|Name=SMED30016771|organism=Schmidtea mediterranea sexual|type=transcript|length=560bp
CGCTATATATGACGTTGGAAATTTCAACAAGAGAGGTTGTGCAATCACAA
ATAATTTTGGGTTACGATCATGAAAATGCAGCCATGTAGAAGATACGTCA
TTCTTCATTTCAACCCTTAACTCTTCATCATTATGTACTTTAATTAACGC
TTCCTGAATATCAGCAGGATAATTCATGAGGTCCAAATTCTCAAGCGAAA
GCACCCAGTCAGGAATATTCAGCTGAACCAAGTCTGCAAAGCGCCTTTCC
ATGTCTCTTATCAATTTTTCCAAGTGAGTCACATAAATCATCAACTCATC
AGCATCAGATTTTTCACTGCTCTCTCGCACAAATCCATCTAATACTGGAA
ACTGCAGAAATAATCGGCGAGCTATGTTGGACCTAAATAGGGTGAACTTC
TTCATAAGACTTAACACAACACGTTTGCCATCTATCAGTGTGACTTTTTG
ACCTTGCAACTGCAGGTTAGACTCATTGAAACGATCAAATACATCAGACA
TGTAGCATATCTGCCAGTGCACAGATGGAATTGCAAGCTTTTCTTTTAAC
CCATCTGAAA
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protein sequence of SMED30016771-orf-1

>SMED30016771-orf-1 ID=SMED30016771-orf-1|Name=SMED30016771-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=186bp
SDGLKEKLAIPSVHWQICYMSDVFDRFNESNLQLQGQKVTLIDGKRVVLS
LMKKFTLFRSNIARRLFLQFPVLDGFVRESSEKSDADELMIYVTHLEKLI
RDMERRFADLVQLNIPDWVLSLENLDLMNYPADIQEALIKVHNDEELRVE
MKNDVSSTWLHFHDRNPKLFVIAQPLLLKFPTSYIA
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