SMED30016710
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016710 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 19
Alignments
SMED30016710 aligns in the following genomic locations:
Homology
BLAST of SMED30016710 vs. Planmine SMEST
Match: SMESG000020037.1 (SMESG000020037.1) HSP 1 Score: 142.51 bits (358), Expect = 4.506e-39 Identity = 73/128 (57.03%), Postives = 92/128 (71.88%), Query Frame = 3 Query: 3690 MNLIRSMSKELRKYRRENSELSAQLFQVSREIQKVKYTWVKPDRTNKIYQQLTATQRGWAEEKQPVQAQRTQIRGLEVVLSACQEDNAITYPLVFAPAQLAHRETHRPDVSLEAKRSRP-RPGKLERA 4070 M + S+EL K RREN+EL QL Q+++EI ++K TWV+P + +YQ++TA QRGWAEEKQ Q Q+ QIR LEV LSACQE NA+TYPLVFAPAQLA+RE + + A + P RPGK ERA Sbjct: 1 MGPYKISSRELEKSRRENAELHQQLIQMTKEIHQIKATWVEPGKVKSLYQKMTAAQRGWAEEKQLHQTQKVQIRSLEVALSACQEGNAVTYPLVFAPAQLAYREA--TETTKPATKPTPRRPGKAERA 126
BLAST of SMED30016710 vs. Planmine SMEST
Match: SMESG000006302.1 (SMESG000006302.1) HSP 1 Score: 123.635 bits (309), Expect = 1.102e-38 Identity = 66/128 (51.56%), Postives = 88/128 (68.75%), Query Frame = 3 Query: 3690 MNLIRSMSKELRKYRRENSELSAQLFQVSREIQKVKYTWVKPDRTNKIYQQLTATQRGWAEEKQPVQAQRTQIRGLEVVLSACQEDNAITYPLVFAPAQLAHRE-THRPDVSLEAKRSRPRPGKLERA 4070 M R++ +EL RR N L AQL Q+S E Q+++ TW++P + +YQ+LTA Q+GW+EEKQ Q+ RTQI+ LEV +SACQE A+TYPLVFAPAQL +RE T P ++ K S PG+ ERA Sbjct: 111 MESARTLQRELDYVRRTNEILRAQLLQLSNETQRIRATWIEPVKMKALYQRLTAAQKGWSEEKQLNQSLRTQIKVLEVAVSACQEGAAVTYPLVFAPAQLVYREATAGPKIT--PKSSGYWPGRKERA 236 HSP 2 Score: 58.151 bits (139), Expect = 1.102e-38 Identity = 41/102 (40.20%), Postives = 59/102 (57.84%), Query Frame = 1 Query: 3358 DDSRITIQISXXXXXXXXQVLWDSGYTISTDRNSDWPIDPRRRNDEIELPAGIIKREPSRNPA*EARQKAELKLASLEAKCKEVQQETATH*STLVKLNGNV 3663 D+SR+TI+ A AA R RQ +WD+G+T+S R+S I R+ +++ELP IKREP N A E R AE LA LE ++ + A L +N ++ Sbjct: 8 DESRVTIRFPAHAADRWRQAMWDAGFTVSQVRDSKSGI--LRQMEDVELPFVAIKREPGPNTAREIRGHAEFLLAHLEEHQRQTGLQVARARLYLANVNEDI 107
BLAST of SMED30016710 vs. Planmine SMEST
Match: SMESG000047732.1 (SMESG000047732.1) HSP 1 Score: 141.739 bits (356), Expect = 1.103e-38 Identity = 67/106 (63.21%), Postives = 85/106 (80.19%), Query Frame = 3 Query: 3684 IKMNLIRSMSKELRKYRRENSELSAQLFQVSREIQKVKYTWVKPDRTNKIYQQLTATQRGWAEEKQPVQAQRTQIRGLEVVLSACQEDNAITYPLVFAPAQLAHRE 4001 I ++ +++SKEL K RREN+EL QL Q++REIQK+K +W++P + + Q+ TATQRGWAEEKQ QAQ+TQIR LEV LSACQE NA+TYPLVF PAQLA+RE Sbjct: 6 IDLDPFKTLSKELVKSRRENAELHRQLLQMTREIQKIKASWMEPTKVKTLSQRFTATQRGWAEEKQLGQAQKTQIRNLEVALSACQEGNAVTYPLVFEPAQLAYRE 111
BLAST of SMED30016710 vs. Planmine SMEST
Match: SMESG000013207.1 (SMESG000013207.1) HSP 1 Score: 140.198 bits (352), Expect = 3.264e-38 Identity = 71/126 (56.35%), Postives = 91/126 (72.22%), Query Frame = 3 Query: 3690 MNLIRSMSKELRKYRRENSELSAQLFQVSREIQKVKYTWVKPDRTNKIYQQLTATQRGWAEEKQPVQAQRTQIRGLEVVLSACQEDNAITYPLVFAPAQLAHRETHRPDVSLEAKRSRPRPGKLER 4067 M +R++S++L K R+EN+EL +QLFQ+SREIQK++ T +P R YQ+LTA QRGW EEKQ Q Q+TQIR LEV LSA QE NA TYPLVFAP Q+A+R+ + + RS+ RPGK ER Sbjct: 1 MESLRTLSRKLVKSRKENTELHSQLFQMSREIQKIRSTSGEPTRMKTTYQRLTAAQRGWTEEKQLAQNQKTQIRSLEVALSASQESNAGTYPLVFAPVQIAYRDAISTTKLVASLRSQRRPGKAER 126
BLAST of SMED30016710 vs. Planmine SMEST
Match: SMESG000036641.1 (SMESG000036641.1) HSP 1 Score: 144.05 bits (362), Expect = 1.125e-37 Identity = 74/127 (58.27%), Postives = 92/127 (72.44%), Query Frame = 3 Query: 3690 MNLIRSMSKELRKYRRENSELSAQLFQVSREIQKVKYTWVKPDRTNKIYQQLTATQRGWAEEKQPVQAQRTQIRGLEVVLSACQEDNAITYPLVFAPAQLAHRETHRPDVSLEAKRSRPRPGKLERA 4070 M +R++SK+L R+EN+EL QL Q+SREIQK++ TW++P R I Q+LTA QRGWAEEKQ VQ QRTQ R LEV L ACQE NA+TY LV APAQ+A+R+ + AKR + RPGK ERA Sbjct: 157 MQSLRTLSKKLMNSRKENTELHNQLIQMSREIQKIRATWIEPARAKAINQRLTAAQRGWAEEKQLVQNQRTQNRSLEVALLACQEGNAVTYQLVIAPAQIAYRDASSTTKPVTAKRPQQRPGKTERA 283 HSP 2 Score: 58.5362 bits (140), Expect = 1.080e-8 Identity = 37/83 (44.58%), Postives = 49/83 (59.04%), Query Frame = 1 Query: 3418 LWDSGYTISTDRNSDWPIDPRR-RNDEIELPAGIIKREPSRNPA*EARQKAELKLASLEAKCKEVQQETATH*STLVKLNGNV 3663 +WD+GY+IS R S + R NDE+ELP IK+EP RNPA EARQ+AE L LE K+ + A L K+N ++ Sbjct: 1 MWDAGYSISPVRESR---ESRHFNNDEVELPYVAIKQEPGRNPAREARQRAESTLDCLEIHQKQTNWQVAMARQWLDKVNADI 80 The following BLAST results are available for this feature:
BLAST of SMED30016710 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30016710 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30016710 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30016710 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30016710 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016710 ID=SMED30016710|Name=SMED30016710|organism=Schmidtea mediterranea sexual|type=transcript|length=4160bpback to top protein sequence of SMED30016710-orf-1 >SMED30016710-orf-1 ID=SMED30016710-orf-1|Name=SMED30016710-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=140bp MNLIRSMSKELRKYRRENSELSAQLFQVSREIQKVKYTWVKPDRTNKIYQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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