Zinc finger CCHC domain-containing protein 2

Overview
NameZinc finger CCHC domain-containing protein 2
Smed IDSMED30016670
Length (bp)2127
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Zinc finger CCHC domain-containing protein 2 (SMED30016670) t-SNE clustered cells

Violin plots show distribution of expression levels for Zinc finger CCHC domain-containing protein 2 (SMED30016670) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Zinc finger CCHC domain-containing protein 2 (SMED30016670) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Zinc finger CCHC domain-containing protein 2 (SMED30016670) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016670

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30016670SMESG000029166.1 Contig5006newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016670SMESG000029166.1 Contig5006uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016670SMESG000021482.1 Contig5006newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016670SMESG000021482.1 Contig5006uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
parenchymal cellSMED30016670SMESG000029166.1 dd_Smed_v4_9698_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30016670SMESG000029166.1 dd_Smed_v4_9698_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016670SMESG000029166.1 dd_Smed_v4_9698_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Cavefish
Match: zcchc14 (zinc finger, CCHC domain containing 14 [Source:ZFIN;Acc:ZDB-GENE-060503-319])

HSP 1 Score: 49.6766 bits (117), Expect = 9.866e-6
Identity = 22/49 (44.90%), Postives = 29/49 (59.18%), Query Frame = 2 
Query: 1490 NLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLIT 1636
             +LDWL++ RLHKY+   + L   EF  L+E+ LN      GA KKL T
Sbjct:  437 GILDWLRKLRLHKYYPVFKQLTMEEFLALTEEDLNKYDLTQGAKKKLKT 485
[Close]
BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Medaka
Match: zcchc14 (zinc finger CCHC-type containing 14 [Source:NCBI gene;Acc:101163975])

HSP 1 Score: 50.8322 bits (120), Expect = 5.724e-6
Identity = 22/49 (44.90%), Postives = 29/49 (59.18%), Query Frame = 2 
Query: 1490 NLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLIT 1636
             +LDWL++ RLHKY+   + L   EF  L+E+ LN      GA KKL T
Sbjct:  540 GILDWLRRLRLHKYYPVFKQLTMEEFLALTEEDLNKYDLTQGAKKKLKT 588
[Close]
BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Medaka
Match: zcchc14 (zinc finger CCHC-type containing 14 [Source:NCBI gene;Acc:101163975])

HSP 1 Score: 50.8322 bits (120), Expect = 5.760e-6
Identity = 22/49 (44.90%), Postives = 29/49 (59.18%), Query Frame = 2 
Query: 1490 NLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLIT 1636
             +LDWL++ RLHKY+   + L   EF  L+E+ LN      GA KKL T
Sbjct:  540 GILDWLRRLRLHKYYPVFKQLTMEEFLALTEEDLNKYDLTQGAKKKLKT 588
[Close]
BLAST of Zinc finger CCHC domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000029166.1 (SMESG000029166.1)

HSP 1 Score: 1086.63 bits (2809), Expect = 0.000e+0
Identity = 603/610 (98.85%), Postives = 605/610 (99.18%), Query Frame = 2 
Query:   68 MKLESYDRLNIIXXXXXXXXXXXXXXXXTIIGSILQYQKLTFDALKEELNHDDNIMFLKNRETIDSHTRSKLISTISMLKVSNVSGATHIFEFLRNNFDKFKEIAHNNTNALEEILLIYQLANDHPAFNFSHCKEIYDWLKEIKSLHVLKDCTEIQLQDELIYDLEVVLIEKNDQQQYIYTIKVKWDRFECCIKRTLEDVYKFYSKLYQMFTTETKAGEICSLYLSYSDNLELSKSLMSKFLKDFLNSPQYVLNCDFVRQFFKPRITDKQICNCDSCRKNFNKLILSQDNRFENIXXXXXXXXXXDNEEYTQSFDIKTEIXXXXXXXXXXXXVVHASNLPLGVSINHNIGSDNASNTSIQRNRSKNRTLHSSGSSPINTPSDESEICMKNDSCKKIYRKISKEINKTNILNTKDLRTENWICQSSNSLAEQTKKKSAIQPYKKSDLEEFDENNVFEEMPVSSREMGPFYSCQIGNLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLITLIRDLKPTDIXXXXXXXXXXXXXXXXXXXXXXXXTFGSCSNISNQNSDLFPDFTTSSAIEPLPPNMIIPQLYYQHFMSMPNLIVLSP 1897
            MKLESYDRLNIISDLLKFSSLFELE+FSTIIGSILQYQKLTFDALKEELNHDDNIMFLKNRETIDSHTRSKLISTISMLKVSNVSGATHIFEFLRNNFDKFKEIAHNNTNALEEILLIYQLANDHPAFNFSHCKEIYDWLKEIKSLHVLKDCTEIQLQDELIYDLEVVLIEKNDQQQYIYTIKVKWDRFECCIKRTLEDVYKFYSKLYQMFTTETKAGEICSLYLSYSDNLELSKSLMSKFLKDFLNSPQYVLNCDFVRQFFKPRITDKQICNCDSCRKNFNKLILSQDNRFENISSCSSPNNPSDNEEYTQSFDIKTEISSYSNSNYSSNSVVHASNLPLGVSINHNIGSDNASNTSIQRNRSKNRTLHSSGSSPINTPSDESEICMKNDSCKKIYRKISKEINKTNILNTKDLRTENWICQSSNSLAEQTKKKSAIQPYKKSDLEEFDEN+VFEEMPVSS EM PFYSCQIGNLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLITLIRDLKPTDINVTNNNVVNLVSRNRSNSNDSSRSTFGSCSNISNQNSDLFPDFTTSSAIEPLPPNMIIPQLYYQHFMSMPNLI   P
Sbjct:    1 MKLESYDRLNIISDLLKFSSLFELEYFSTIIGSILQYQKLTFDALKEELNHDDNIMFLKNRETIDSHTRSKLISTISMLKVSNVSGATHIFEFLRNNFDKFKEIAHNNTNALEEILLIYQLANDHPAFNFSHCKEIYDWLKEIKSLHVLKDCTEIQLQDELIYDLEVVLIEKNDQQQYIYTIKVKWDRFECCIKRTLEDVYKFYSKLYQMFTTETKAGEICSLYLSYSDNLELSKSLMSKFLKDFLNSPQYVLNCDFVRQFFKPRITDKQICNCDSCRKNFNKLILSQDNRFENISSCSSPNNPSDNEEYTQSFDIKTEISSYSNSNYSSNSVVHASNLPLGVSINHNIGSDNASNTSIQRNRSKNRTLHSSGSSPINTPSDESEICMKNDSCKKIYRKISKEINKTNILNTKDLRTENWICQSSNSLAEQTKKKSAIQPYKKSDLEEFDENSVFEEMPVSSGEMSPFYSCQIGNLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLITLIRDLKPTDINVTNNNVVNLVSRNRSNSNDSSRSTFGSCSNISNQNSDLFPDFTTSSAIEPLPPNMIIPQLYYQHFMSMPNLIFHKP 610

HSP 2 Score: 111.694 bits (278), Expect = 2.551e-25
Identity = 52/53 (98.11%), Postives = 52/53 (98.11%), Query Frame = 3 
Query: 1932 FHKPSSRLFVRITPIPDSSCKSPRYPSHNWNQQYVLMRVELKHKLVNVVLFYK 2090
            FHKPSSRLFVRIT IPDSSCKSPRYPSHNWNQQYVLMRVELKHKLVNVVLFYK
Sbjct:  607 FHKPSSRLFVRITLIPDSSCKSPRYPSHNWNQQYVLMRVELKHKLVNVVLFYK 659
[Close]
BLAST of Zinc finger CCHC domain-containing protein 2 vs. Planmine SMEST
Match: SMESG000029166.1 (SMESG000029166.1)

HSP 1 Score: 1059.28 bits (2738), Expect = 0.000e+0
Identity = 596/621 (95.97%), Postives = 598/621 (96.30%), Query Frame = 2 
Query:   35 MVNKDVLFQTFMKLESYDRLNIIXXXXXXXXXXXXXXXXTIIGSILQYQKLTFDALKEELNHDDNIMFLKNRETIDSHTRSKLISTISMLKVSNVSGATHIFEFLRNNFDKFKEIAHNNTNALEEILLIYQLANDHPAFNFSHCKEIYDWLKEIKSLHVLKDCTEIQLQDELIYDLEVVLIEKNDQQQYIYTIKVKWDRFECCIKRTLEDVYKFYSKLYQMFTTETKAGEICSLYLSYSDNLELSKSLMSKFLKDFLNSPQYVLNCDFVRQFFKPRITDKQICNCDSCRKNFNKLILSQDNRFENIXXXXXXXXXXDNEEYTQSFDIKTEIXXXXXXXXXXXXVVHASNLPLGVSINHNIGSDNASNTSIQRNRSKNRTLHSSGSSPINTPSDESEICMKNDSCKKIYRKISKEINKTNILNTKDLRTENWICQSSNSLAEQTKKKSAIQPYKKSDLEEFDENNVFEEMPVSSREMGPFYSCQIGNLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLITLIRDLKPTDIXXXXXXXXXXXXXXXXXXXXXXXXTFGSCSNISNQNSDLFPDFTTSSAIEPLPPNMIIPQLYYQHFMSMPNLIVLSP 1897
            MVNKDVLFQTFMKLESYDRLNIISDLLKFSSLFELE+FSTIIGSILQYQKLTFDALKEELNHDDNIMFLKNRETIDSHTRSKLISTISMLKVSNVSGATHIFEFLRNNFDKFKEIAHNNTNALEEILLIYQLANDHPAFNFSHCKEIYDWLKEIKSLHVLKDCTEIQLQDELIYDLEVVLIEKNDQQQYIYTIKVKWDRFECCIKRTLEDVYKFYSKLYQMFTTETKAGEICSLYLSYSDNLELSKSLMSKFLKDFLNSPQYVLNCDFVRQFFKPRITDKQI                  NRFENISSCSSPNNPSDNEEYTQSFDIKTEISSYSNSNYSSNSVVHASNLPLGVSINHNIGSDNASNTSIQRNRSKNRTLHSSGSSPINTPSDESEICMKNDSCKKIYRKISKEINKTNILNTKDLRTENWICQSSNSLAEQTKKKSAIQPYKKSDLEEFDEN+VFEEMPVSS EM PFYSCQIGNLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLITLIRDLKPTDINVTNNNVVNLVSRNRSNSNDSSRSTFGSCSNISNQNSDLFPDFTTSSAIEPLPPNMIIPQLYYQHFMSMPNLI   P
Sbjct:    1 MVNKDVLFQTFMKLESYDRLNIISDLLKFSSLFELEYFSTIIGSILQYQKLTFDALKEELNHDDNIMFLKNRETIDSHTRSKLISTISMLKVSNVSGATHIFEFLRNNFDKFKEIAHNNTNALEEILLIYQLANDHPAFNFSHCKEIYDWLKEIKSLHVLKDCTEIQLQDELIYDLEVVLIEKNDQQQYIYTIKVKWDRFECCIKRTLEDVYKFYSKLYQMFTTETKAGEICSLYLSYSDNLELSKSLMSKFLKDFLNSPQYVLNCDFVRQFFKPRITDKQIY-----------------NRFENISSCSSPNNPSDNEEYTQSFDIKTEISSYSNSNYSSNSVVHASNLPLGVSINHNIGSDNASNTSIQRNRSKNRTLHSSGSSPINTPSDESEICMKNDSCKKIYRKISKEINKTNILNTKDLRTENWICQSSNSLAEQTKKKSAIQPYKKSDLEEFDENSVFEEMPVSSGEMSPFYSCQIGNLLDWLKQSRLHKYHSFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLITLIRDLKPTDINVTNNNVVNLVSRNRSNSNDSSRSTFGSCSNISNQNSDLFPDFTTSSAIEPLPPNMIIPQLYYQHFMSMPNLIFHKP 604

HSP 2 Score: 112.079 bits (279), Expect = 2.249e-25
Identity = 52/53 (98.11%), Postives = 52/53 (98.11%), Query Frame = 3 
Query: 1932 FHKPSSRLFVRITPIPDSSCKSPRYPSHNWNQQYVLMRVELKHKLVNVVLFYK 2090
            FHKPSSRLFVRIT IPDSSCKSPRYPSHNWNQQYVLMRVELKHKLVNVVLFYK
Sbjct:  601 FHKPSSRLFVRITLIPDSSCKSPRYPSHNWNQQYVLMRVELKHKLVNVVLFYK 653
The following BLAST results are available for this feature:
BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zcchc149.866e-644.90zinc finger, CCHC domain containing 14 [Source:ZFI... [more]
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
zcchc145.724e-644.90zinc finger CCHC-type containing 14 [Source:NCBI g... [more]
zcchc145.760e-644.90zinc finger CCHC-type containing 14 [Source:NCBI g... [more]
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BLAST of Zinc finger CCHC domain-containing protein 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000029166.12.551e-2598.85SMESG000029166.1[more]
SMESG000029166.12.249e-2595.97SMESG000029166.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016670 ID=SMED30016670|Name=Zinc finger CCHC domain-containing protein 2|organism=Schmidtea mediterranea sexual|type=transcript|length=2127bp
TATAAATCTTATATGATAAATTTATTATAGAGAGATGGTCAATAAAGATG
TTTTATTTCAAACCTTTATGAAATTAGAAAGTTATGACAGACTTAACATT
ATATCAGATTTACTAAAATTTTCCAGTTTATTTGAGTTAGAATTTTTTTC
AACTATTATTGGAAGTATTTTACAATATCAAAAATTAACATTCGATGCTC
TGAAAGAAGAATTGAATCACGATGACAATATTATGTTTTTAAAAAACAGA
GAAACTATTGATTCACACACCAGATCCAAATTAATATCTACTATTTCTAT
GCTCAAAGTGTCAAATGTATCAGGGGCAACTCATATATTTGAATTTTTAC
GAAACAACTTTGATAAATTTAAAGAAATAGCTCACAATAACACAAATGCT
CTTGAAGAAATTCTTCTAATTTATCAATTAGCAAATGATCATCCTGCTTT
TAATTTCAGTCACTGTAAGGAAATATATGACTGGTTAAAAGAAATTAAAT
CCCTACATGTTCTGAAAGATTGTACAGAAATTCAGCTTCAAGATGAACTC
ATATATGATCTCGAGGTGGTACTCATTGAAAAGAACGACCAGCAACAGTA
TATTTATACAATAAAAGTCAAATGGGACCGATTTGAGTGTTGCATAAAAC
GAACCCTGGAAGATGTGTATAAATTTTATTCAAAGCTTTATCAAATGTTT
ACTACCGAAACGAAAGCCGGTGAAATTTGCTCTCTATATCTCAGTTATTC
TGATAATCTCGAATTGAGTAAATCACTAATGTCGAAATTTCTAAAAGACT
TTTTGAATTCACCCCAGTACGTGTTGAATTGCGATTTCGTTCGGCAATTC
TTCAAGCCAAGAATCACAGATAAACAAATTTGTAATTGTGATTCATGCAG
AAAAAACTTTAATAAATTGATTTTGTCACAAGACAATCGATTTGAAAATA
TTAGCAGTTGCAGCAGCCCTAATAACCCATCAGACAATGAAGAATACACT
CAGTCTTTTGATATTAAAACTGAAATTTCATCATACTCAAACTCAAATTA
CTCCTCCAATTCTGTGGTTCACGCTTCCAATTTACCTCTTGGCGTTTCAA
TCAATCACAATATAGGTTCTGATAACGCAAGCAATACTTCAATCCAGCGT
AATAGATCTAAAAATCGAACGCTTCATAGTTCCGGTTCTTCTCCAATTAA
CACACCGTCAGACGAATCTGAGATATGCATGAAAAATGACTCATGTAAGA
AAATTTACCGAAAAATCAGTAAAGAAATTAACAAAACCAATATTTTAAAC
ACTAAAGATCTCAGAACAGAAAATTGGATTTGTCAGTCTTCAAATTCACT
TGCAGAACAAACTAAAAAAAAATCGGCTATTCAGCCATACAAAAAATCAG
ATTTAGAAGAATTTGATGAAAACAATGTATTCGAAGAAATGCCAGTTTCG
TCAAGAGAAATGGGCCCGTTCTATTCTTGTCAAATTGGTAATTTACTCGA
TTGGCTTAAACAAAGTCGATTGCATAAATATCACTCATTTATCCAAAATT
TGAATTTCGGAGAGTTCCACAATCTCTCTGAAGACCAACTAAACAATCTC
GGATTCGTCGACGGCGCATCAAAAAAATTAATCACACTTATTCGCGACCT
AAAACCAACGGATATTAACGTCACAAATAATAACGTGGTGAATTTAGTGT
CTAGAAATCGATCGAATTCAAACGATTCCTCTCGGTCAACTTTTGGATCA
TGTAGTAATATTTCTAATCAGAATTCCGATCTGTTTCCTGATTTCACTAC
ATCAAGCGCGATTGAACCACTCCCTCCGAATATGATTATTCCACAATTAT
ACTATCAACATTTCATGTCTATGCCCAATCTAATAGTGCTTTCACCAAAT
TTTATGTCGAATCCACAAAGTTTGTATTCAGTTTCACAAGCCAAGCAGCA
GATTATTCGTTCGCATAACACCAATTCCTGATTCCAGCTGTAAATCTCCC
CGCTATCCTTCTCATAACTGGAATCAGCAATATGTGCTCATGCGTGTTGA
ACTGAAACACAAATTAGTTAATGTTGTATTATTTTATAAATAAATCAATA
ATTCCAGTTCCAAAGAAGGATGTGTAT
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protein sequence of SMED30016670-orf-1

>SMED30016670-orf-1 ID=SMED30016670-orf-1|Name=SMED30016670-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=649bp
MVNKDVLFQTFMKLESYDRLNIISDLLKFSSLFELEFFSTIIGSILQYQK
LTFDALKEELNHDDNIMFLKNRETIDSHTRSKLISTISMLKVSNVSGATH
IFEFLRNNFDKFKEIAHNNTNALEEILLIYQLANDHPAFNFSHCKEIYDW
LKEIKSLHVLKDCTEIQLQDELIYDLEVVLIEKNDQQQYIYTIKVKWDRF
ECCIKRTLEDVYKFYSKLYQMFTTETKAGEICSLYLSYSDNLELSKSLMS
KFLKDFLNSPQYVLNCDFVRQFFKPRITDKQICNCDSCRKNFNKLILSQD
NRFENISSCSSPNNPSDNEEYTQSFDIKTEISSYSNSNYSSNSVVHASNL
PLGVSINHNIGSDNASNTSIQRNRSKNRTLHSSGSSPINTPSDESEICMK
NDSCKKIYRKISKEINKTNILNTKDLRTENWICQSSNSLAEQTKKKSAIQ
PYKKSDLEEFDENNVFEEMPVSSREMGPFYSCQIGNLLDWLKQSRLHKYH
SFIQNLNFGEFHNLSEDQLNNLGFVDGASKKLITLIRDLKPTDINVTNNN
VVNLVSRNRSNSNDSSRSTFGSCSNISNQNSDLFPDFTTSSAIEPLPPNM
IIPQLYYQHFMSMPNLIVLSPNFMSNPQSLYSVSQAKQQIIRSHNTNS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000478pharynx musculature
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036871PX_dom_sf
IPR042344ZCCHC14
IPR001683Phox
IPR013761SAM/pointed_sf
Vocabulary: molecular function
TermDefinition
GO:0035091phosphatidylinositol binding
GO:0003676nucleic acid binding
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036871PX domain superfamilyGENE3DG3DSA:3.30.1520.10coord: 169..291
e-value: 7.0E-11
score: 44.2
IPR036871PX domain superfamilySUPERFAMILYSSF64268PX domaincoord: 185..290
IPR013761Sterile alpha motif/pointed domain superfamilyGENE3DG3DSA:1.10.150.50coord: 481..540
e-value: 1.4E-11
score: 46.3
IPR013761Sterile alpha motif/pointed domain superfamilySUPERFAMILYSSF47769SAM/Pointed domaincoord: 484..540
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..394
IPR042344Zinc finger CCHC domain-containing protein 14PANTHERPTHR16195ZINC FINGER CCHC DOMAIN CONTAINING PROTEINcoord: 1..533