Protein big brother

Overview


Name	Protein big brother
Smed ID	SMED30016591
Length (bp)	870

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Protein big brother (SMED30016591) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein big brother (SMED30016591) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein big brother (SMED30016591) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein big brother (SMED30016591) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30016591

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 15

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pigment cell	SMED30016591	h1SMcG0016233	SmedASXL_018544	SmedAsxl_ww_GCZZ01	PMID:29158443 He et al., 2017
X1 cell	SMED30016591	h1SMcG0016233	SmedASXL_018544	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30016591	h1SMcG0016233	SmedASXL_018544	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
pharynx	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30016591	h1SMcG0016233	dd_Smed_v4_2875_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30016591	h1SMcG0016233	SMED_19615_V2_1	GPL14150	PMID:22385657 Wagner et al., 2012
neoblast	SMED30016591	h1SMcG0016233	SMED_19615_V2_1	GPL14150_gene_models	PMID:22385657 Wagner et al., 2012

Note: Hover over icons to view figure legend

Homology

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BLAST of Protein big brother vs. Ensembl Human
Match: CBFB (core-binding factor subunit beta [Source:HGNC Symbol;Acc:HGNC:1539])

HSP 1 Score: 87.0409 bits (214), Expect = 8.159e-20
Identity = 51/135 (37.78%), Postives = 75/135 (55.56%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLS------DNENIRFDDEER----VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEE 585
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L     S         +  + D ER    V+L++ +ILNGVCV W G I+L+ L+G   +EFD + AQ+E
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQE 135

[Close]

BLAST of Protein big brother vs. Ensembl Human
Match: CBFB (core-binding factor subunit beta [Source:HGNC Symbol;Acc:HGNC:1539])

HSP 1 Score: 85.8853 bits (211), Expect = 1.002e-19
Identity = 51/135 (37.78%), Postives = 75/135 (55.56%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLS------DNENIRFDDEER----VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEE 585
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L     S         +  + D ER    V+L++ +ILNGVCV W G I+L+ L+G   +EFD + AQ+E
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQE 135

[Close]

BLAST of Protein big brother vs. Ensembl Human
Match: CBFB (core-binding factor subunit beta [Source:HGNC Symbol;Acc:HGNC:1539])

HSP 1 Score: 84.7297 bits (208), Expect = 2.607e-19
Identity = 51/137 (37.23%), Postives = 75/137 (54.74%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLS------DNENIRFDDEER----VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L     S         +  + D ER    V+L++ +ILNGVCV W G I+L+ L+G   +EFD + AQ+E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Human
Match: CBFB (core-binding factor subunit beta [Source:HGNC Symbol;Acc:HGNC:1539])

HSP 1 Score: 65.4698 bits (158), Expect = 4.194e-13
Identity = 40/125 (32.00%), Postives = 61/125 (48.80%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEE 585
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  I                             V+L++ +ILNGVCV W G I+L+ L+G   +EFD + AQ+E
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEI-----------------------------VYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQE 96

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BLAST of Protein big brother vs. Ensembl Celegans
Match: bro-1 (BROther (Drosophila tx factor partner) homolog [Source:UniProtKB/TrEMBL;Acc:Q95ZL9])

HSP 1 Score: 45.0542 bits (105), Expect = 8.706e-6
Identity = 29/116 (25.00%), Postives = 52/116 (44.83%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSGE--NAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIE 558
            M +T  DQ+  F+ D    +LS     +++  +       +  F   ++  ++ + F  +GIN+ + +     N N      E  F    L+ NG+ VK +G +N + LEG  R E
Sbjct:    1 MKRTTTDQQSSFQTDYFLTQLSHFTEAKFSLFEHAPLSERRERFRVHVERDEMPLTFCKTGINIPVKLEWSQTNGN------EISFRSRPLVFNGIWVKVIGAMNSESLEGRVRFE 110

[Close]

BLAST of Protein big brother vs. Ensembl Fly
Match: Bro (gene:FBgn0013755 transcript:FBtr0072760)

HSP 1 Score: 101.679 bits (252), Expect = 1.137e-25
Identity = 56/148 (37.84%), Postives = 86/148 (58.11%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITG----LSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYN 621
            P F  KMP+ V DQ  KF  DE+F+RLS ++ +RYTG +  + E  +  F+ D + G   I+ V SG NL+L         +  ++  F+ E  +V L+S  I+NGVCV++ G ++L  L+G+A +EFD + AQ+E      Q+  YN
Sbjct:   31 PRFIFKMPRVVPDQRSKFDSDELFRRLSRESEVRYTGYRERAMEERRMRFVNDCRKGYAEISMVASGTNLQLYFNANHNPYAQEQDCDFERERGKVHLRSSFIMNGVCVRFRGWVDLDRLDGAACLEFDEQRAQQEDAQLQEQIQSYN 178

[Close]

BLAST of Protein big brother vs. Ensembl Fly
Match: Bgb (gene:FBgn0013753 transcript:FBtr0072758)

HSP 1 Score: 97.0561 bits (240), Expect = 6.139e-24
Identity = 58/162 (35.80%), Postives = 89/162 (54.94%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGC--KSGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEE--------RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNK 654
            P F  KMP+ V DQ+ KF+ DE+F+RLS ++ +RYTG   +S E  +  FM   + G    +FV SG NL+L       N+N    D+E        +V ++S+ I+NGVCV++ G I+L+ L+G   +E+D + A  E     +Q+  YN      ED  +
Sbjct:   24 PRFIFKMPRVVPDQKSKFESDELFRRLSRESEVRYTGYRERSIEERQVRFMNGCREGHTEASFVASGTNLQLVFNA---NQNPYLHDKECDFDKEHGKVHIKSYFIMNGVCVRFRGWIDLERLDGVGCLEYDERRAMHEDAILRDQIDRYNQRLREFEDTKR 182

[Close]

BLAST of Protein big brother vs. Ensembl Fly
Match: Bgb (gene:FBgn0013753 transcript:FBtr0330180)

HSP 1 Score: 97.0561 bits (240), Expect = 1.663e-23
Identity = 58/162 (35.80%), Postives = 89/162 (54.94%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGC--KSGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEE--------RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNK 654
            P F  KMP+ V DQ+ KF+ DE+F+RLS ++ +RYTG   +S E  +  FM   + G    +FV SG NL+L       N+N    D+E        +V ++S+ I+NGVCV++ G I+L+ L+G   +E+D + A  E     +Q+  YN      ED  +
Sbjct:   24 PRFIFKMPRVVPDQKSKFESDELFRRLSRESEVRYTGYRERSIEERQVRFMNGCREGHTEASFVASGTNLQLVFNA---NQNPYLHDKECDFDKEHGKVHIKSYFIMNGVCVRFRGWIDLERLDGVGCLEYDERRAMHEDAILRDQIDRYNQRLREFEDTKR 182

[Close]

BLAST of Protein big brother vs. Ensembl Zebrafish
Match: cbfb (core-binding factor subunit beta [Source:ZFIN;Acc:ZDB-GENE-980526-440])

HSP 1 Score: 83.1889 bits (204), Expect = 5.965e-19
Identity = 49/137 (35.77%), Postives = 74/137 (54.01%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRL---------DITGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L         D       E + F+ E  +V+L++ +ILNGVCV W G ++L  L+G   +E+D + AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPANLHGDQRQAPTREYVDFERETGKVYLKAPMILNGVCVIWRGWLDLHRLDGMGCLEYDDERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Zebrafish
Match: cbfb (core-binding factor subunit beta [Source:ZFIN;Acc:ZDB-GENE-980526-440])

HSP 1 Score: 83.1889 bits (204), Expect = 7.736e-19
Identity = 49/137 (35.77%), Postives = 74/137 (54.01%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRL---------DITGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L         D       E + F+ E  +V+L++ +ILNGVCV W G ++L  L+G   +E+D + AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPANLHGDQRQAPTREYVDFERETGKVYLKAPMILNGVCVIWRGWLDLHRLDGMGCLEYDDERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Zebrafish
Match: cbfb (core-binding factor subunit beta [Source:ZFIN;Acc:ZDB-GENE-980526-440])

HSP 1 Score: 83.1889 bits (204), Expect = 8.071e-19
Identity = 49/137 (35.77%), Postives = 74/137 (54.01%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRL---------DITGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L         D       E + F+ E  +V+L++ +ILNGVCV W G ++L  L+G   +E+D + AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPANLHGDQRQAPTREYVDFERETGKVYLKAPMILNGVCVIWRGWLDLHRLDGMGCLEYDDERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Zebrafish
Match: cbfb (core-binding factor subunit beta [Source:ZFIN;Acc:ZDB-GENE-980526-440])

HSP 1 Score: 83.1889 bits (204), Expect = 8.071e-19
Identity = 49/137 (35.77%), Postives = 74/137 (54.01%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRL---------DITGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L         D       E + F+ E  +V+L++ +ILNGVCV W G ++L  L+G   +E+D + AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPANLHGDQRQAPTREYVDFERETGKVYLKAPMILNGVCVIWRGWLDLHRLDGMGCLEYDDERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Zebrafish
Match: cbfb (core-binding factor subunit beta [Source:ZFIN;Acc:ZDB-GENE-980526-440])

HSP 1 Score: 82.4185 bits (202), Expect = 1.214e-18
Identity = 49/137 (35.77%), Postives = 74/137 (54.01%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRL---------DITGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L         D       E + F+ E  +V+L++ +ILNGVCV W G ++L  L+G   +E+D + AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPANLHGDQRQAPTREYVDFERETGKVYLKAPMILNGVCVIWRGWLDLHRLDGMGCLEYDDERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Xenopus
Match: or1s1 (olfactory receptor family 1 subfamily S member 1 (gene/pseudogene) [Source:Xenbase;Acc:XB-GENE-5904137])

HSP 1 Score: 82.8037 bits (203), Expect = 1.231e-18
Identity = 49/137 (35.77%), Postives = 74/137 (54.01%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRL---------DITGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++L+ ++ I+YTG +    E  +  F    + G+  IAFV +G NL L         +       E + F+ E  +V L++ +ILNGVCV W G I+L+ L+G   +EFD   AQ E  
Sbjct:    1 MPRVVPDQRAKFENEEFFRKLNRESEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPANWQGEPRQAPTREYVDFEREPGKVHLKAPMILNGVCVLWKGWIDLQRLDGMGCLEFDDDRAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Mouse
Match: Cbfb (core binding factor beta [Source:MGI Symbol;Acc:MGI:99851])

HSP 1 Score: 85.8853 bits (211), Expect = 8.111e-20
Identity = 51/135 (37.78%), Postives = 74/135 (54.81%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLS------DNENIRFDDEER----VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEE 585
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L     S         +  + D ER    V+L++ +ILNGVCV W G I+L  L+G   +EFD + AQ+E
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQQE 135

[Close]

BLAST of Protein big brother vs. Ensembl Mouse
Match: Cbfb (core binding factor beta [Source:MGI Symbol;Acc:MGI:99851])

HSP 1 Score: 84.7297 bits (208), Expect = 2.067e-19
Identity = 51/137 (37.23%), Postives = 74/137 (54.01%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLS------DNENIRFDDEER----VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L     S         +  + D ER    V+L++ +ILNGVCV W G I+L  L+G   +EFD + AQ+E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQQEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Mouse
Match: Cbfb (core binding factor beta [Source:MGI Symbol;Acc:MGI:99851])

HSP 1 Score: 83.1889 bits (204), Expect = 3.904e-19
Identity = 50/133 (37.59%), Postives = 72/133 (54.14%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLS------DNENIRFDDEER----VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQ 579
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L     S         +  + D ER    V+L++ +ILNGVCV W G I+L  L+G   +EFD + AQ
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQ 133

[Close]

BLAST of Protein big brother vs. Ensembl Mouse
Match: Cbfb (core binding factor beta [Source:MGI Symbol;Acc:MGI:99851])

HSP 1 Score: 65.0846 bits (157), Expect = 1.195e-12
Identity = 40/127 (31.50%), Postives = 60/127 (47.24%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  I                             V+L++ +ILNGVCV W G I+L  L+G   +EFD + AQ+E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEI-----------------------------VYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQQEDA 98

[Close]

BLAST of Protein big brother vs. UniProt/SwissProt
Match: sp|Q24039|BRO_DROME (Protein brother OS=Drosophila melanogaster OX=7227 GN=Bro PE=1 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 1.140e-24
Identity = 56/148 (37.84%), Postives = 86/148 (58.11%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITG----LSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYN 621
            P F  KMP+ V DQ  KF  DE+F+RLS ++ +RYTG +  + E  +  F+ D + G   I+ V SG NL+L         +  ++  F+ E  +V L+S  I+NGVCV++ G ++L  L+G+A +EFD + AQ+E      Q+  YN
Sbjct:   31 PRFIFKMPRVVPDQRSKFDSDELFRRLSRESEVRYTGYRERAMEERRMRFVNDCRKGYAEISMVASGTNLQLYFNANHNPYAQEQDCDFERERGKVHLRSSFIMNGVCVRFRGWVDLDRLDGAACLEFDEQRAQQEDAQLQEQIQSYN 178

[Close]

BLAST of Protein big brother vs. UniProt/SwissProt
Match: sp|Q24040|BGB_DROME (Protein big brother OS=Drosophila melanogaster OX=7227 GN=Bgb PE=2 SV=3)

HSP 1 Score: 97.0561 bits (240), Expect = 6.154e-23
Identity = 58/162 (35.80%), Postives = 89/162 (54.94%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGC--KSGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEE--------RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNK 654
            P F  KMP+ V DQ+ KF+ DE+F+RLS ++ +RYTG   +S E  +  FM   + G    +FV SG NL+L       N+N    D+E        +V ++S+ I+NGVCV++ G I+L+ L+G   +E+D + A  E     +Q+  YN      ED  +
Sbjct:   24 PRFIFKMPRVVPDQKSKFESDELFRRLSRESEVRYTGYRERSIEERQVRFMNGCREGHTEASFVASGTNLQLVFNA---NQNPYLHDKECDFDKEHGKVHIKSYFIMNGVCVRFRGWIDLERLDGVGCLEYDERRAMHEDAILRDQIDRYNQRLREFEDTKR 182

[Close]

BLAST of Protein big brother vs. UniProt/SwissProt
Match: sp|Q13951|PEBB_HUMAN (Core-binding factor subunit beta OS=Homo sapiens OX=9606 GN=CBFB PE=1 SV=2)

HSP 1 Score: 85.8853 bits (211), Expect = 4.811e-19
Identity = 51/135 (37.78%), Postives = 75/135 (55.56%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLS------DNENIRFDDEER----VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEE 585
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L     S         +  + D ER    V+L++ +ILNGVCV W G I+L+ L+G   +EFD + AQ+E
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQE 135

[Close]

BLAST of Protein big brother vs. UniProt/SwissProt
Match: sp|Q08024|PEBB_MOUSE (Core-binding factor subunit beta OS=Mus musculus OX=10090 GN=Cbfb PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.446e-18
Identity = 51/137 (37.23%), Postives = 74/137 (54.01%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLS------DNENIRFDDEER----VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L     S         +  + D ER    V+L++ +ILNGVCV W G I+L  L+G   +EFD + AQ+E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQQEDA 137

[Close]

BLAST of Protein big brother vs. TrEMBL
Match: E2BIL2 (Protein big brother OS=Harpegnathos saltator OX=610380 GN=EAI_13149 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.228e-23
Identity = 61/149 (40.94%), Postives = 85/149 (57.05%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIR-----FDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYN 621
            P    KMP+ V DQ+ KF+ DE+F+RLS ++ +RYTG +    E  +  F    + G   IAF  +G NL+L   G S N  +      FD E  +V L+SHLI+NGVCV+W G I+L+ L+G   +E+D + A EE      QL  YN
Sbjct:   28 PRLVFKMPRVVPDQKAKFESDELFRRLSRESEVRYTGYRDRPMEERQVRFQNGCREGHTEIAFAATGTNLQLVFGGASGNYPVDPCDCDFDKEHGKVHLRSHLIMNGVCVRWRGWIDLERLDGVGCLEYDEERAAEEDALLREQLERYN 176

[Close]

BLAST of Protein big brother vs. TrEMBL
Match: A0A158NKA4 (Uncharacterized protein OS=Atta cephalotes OX=12957 GN=105621100 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.783e-23
Identity = 60/149 (40.27%), Postives = 85/149 (57.05%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSG--ENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIR-----FDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYN 621
            P    KMP+ V DQ+ KF+ DE+F+RLS ++ +RYTG +    E  +  F    + G   IAF  +G NL+L     S N  +      FD E  +V L+SHLI+NGVCV+W G I+L+ L+G   +E+D + A EE     +QL  YN
Sbjct:   28 PRLVFKMPRVVPDQKAKFESDELFRRLSRESEVRYTGYRDRPIEERQVRFQNGCREGHTEIAFAATGTNLQLVFGNASGNYPVDPNDCDFDKEHGKVHLRSHLIMNGVCVRWRGWIDLERLDGVGCLEYDEEHAAEEDALLRDQLERYN 176

[Close]

BLAST of Protein big brother vs. TrEMBL
Match: A0A151WIS2 (Protein big brother OS=Trachymyrmex zeteki OX=64791 GN=ALC60_13246 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.783e-23
Identity = 60/149 (40.27%), Postives = 85/149 (57.05%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSG--ENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIR-----FDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYN 621
            P    KMP+ V DQ+ KF+ DE+F+RLS ++ +RYTG +    E  +  F    + G   IAF  +G NL+L     S N  +      FD E  +V L+SHLI+NGVCV+W G I+L+ L+G   +E+D + A EE     +QL  YN
Sbjct:   28 PRLVFKMPRVVPDQKAKFESDELFRRLSRESEVRYTGYRDRPIEERQVRFQNGCREGHTEIAFAATGTNLQLVFGNASGNYPVDPNDCDFDKEHGKVHLRSHLIMNGVCVRWRGWIDLERLDGVGCLEYDEEHAAEEDALLRDQLERYN 176

[Close]

BLAST of Protein big brother vs. TrEMBL
Match: A0A195EIT1 (Protein big brother OS=Trachymyrmex cornetzi OX=471704 GN=ALC57_02833 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.783e-23
Identity = 60/149 (40.27%), Postives = 85/149 (57.05%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSG--ENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIR-----FDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYN 621
            P    KMP+ V DQ+ KF+ DE+F+RLS ++ +RYTG +    E  +  F    + G   IAF  +G NL+L     S N  +      FD E  +V L+SHLI+NGVCV+W G I+L+ L+G   +E+D + A EE     +QL  YN
Sbjct:   28 PRLVFKMPRVVPDQKAKFESDELFRRLSRESEVRYTGYRDRPIEERQVRFQNGCREGHTEIAFAATGTNLQLVFGNASGNYPVDPNDCDFDKEHGKVHLRSHLIMNGVCVRWRGWIDLERLDGVGCLEYDEEHAAEEDALLRDQLERYN 176

[Close]

BLAST of Protein big brother vs. TrEMBL
Match: A0A195BAT4 (Protein big brother OS=Atta colombica OX=520822 GN=ALC53_08098 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.783e-23
Identity = 60/149 (40.27%), Postives = 85/149 (57.05%), Query Frame = 1

Query:  199 PDFHLKMPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSG--ENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIR-----FDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYN 621
            P    KMP+ V DQ+ KF+ DE+F+RLS ++ +RYTG +    E  +  F    + G   IAF  +G NL+L     S N  +      FD E  +V L+SHLI+NGVCV+W G I+L+ L+G   +E+D + A EE     +QL  YN
Sbjct:   28 PRLVFKMPRVVPDQKAKFESDELFRRLSRESEVRYTGYRDRPIEERQVRFQNGCREGHTEIAFAATGTNLQLVFGNASGNYPVDPNDCDFDKEHGKVHLRSHLIMNGVCVRWRGWIDLERLDGVGCLEYDEEHAAEEDALLRDQLERYN 176

[Close]

BLAST of Protein big brother vs. Ensembl Cavefish
Match: cbfb (core-binding factor subunit beta [Source:NCBI gene;Acc:103026750])

HSP 1 Score: 84.7297 bits (208), Expect = 1.247e-19
Identity = 49/137 (35.77%), Postives = 75/137 (54.74%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITG---------LSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L             +   E + F+ E  +V+L++ +ILNGVCV W G I+L+ L+G   +E+D + AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPANLHGEQRQMPTREYVDFERETGKVYLKAPMILNGVCVIWRGWIDLQRLDGMGCLEYDDERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Cavefish
Match: cbfb (core-binding factor subunit beta [Source:NCBI gene;Acc:103026750])

HSP 1 Score: 83.9593 bits (206), Expect = 2.798e-19
Identity = 49/137 (35.77%), Postives = 75/137 (54.74%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDI---------TGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  IAFV +G NL L             +   E + F+ E  +V+L++ +ILNGVCV W G I+L+ L+G   +E+D + AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPANLHGEQRQMPTREYVDFERETGKVYLKAPMILNGVCVIWRGWIDLQRLDGMGCLEYDDERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000610.1 (pep scaffold:Pmarinus_7.0:GL478173:5823:25505:-1 gene:ENSPMAG00000000551.1 transcript:ENSPMAT00000000610.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.4394 bits (106), Expect = 8.006e-7
Identity = 20/43 (46.51%), Postives = 32/43 (74.42%), Query Frame = 1

Query:  463 VFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            V L++ +ILNGVCV W G ++L+ L+G A +EFD + A++E +
Sbjct:    2 VHLKAPMILNGVCVIWRGWVDLQRLDGMACLEFDEERARQEDL 44

[Close]

BLAST of Protein big brother vs. Ensembl Nematostella
Match: CBFBETA (Core-binding factor subunit beta; Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV13])

HSP 1 Score: 90.1225 bits (222), Expect = 4.449e-22
Identity = 50/130 (38.46%), Postives = 82/130 (63.08%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSD----NENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEE 585
            MP+ V +Q+ KF+ DE+F++L+ ++ I+YTG +  S E     F  +++ G+ N+A+V SG NL L      D    +E + FD E  +V ++SH ILNGVC+ + G I+L+ L+G   IE+D + A++E
Sbjct:    1 MPRVVPEQKQKFENDEMFRKLARESEIKYTGYRDRSHEERVVRFQTEIRDGQSNVAYVASGTNLTLHFPKAEDGFIRSEFLDFDREPGKVHIKSHFILNGVCIIFKGWIDLQRLDGIGYIEYDEEKARKE 130

[Close]

BLAST of Protein big brother vs. Ensembl Medaka
Match: cbfb (core-binding factor subunit beta [Source:NCBI gene;Acc:101157051])

HSP 1 Score: 87.0409 bits (214), Expect = 1.477e-20
Identity = 49/137 (35.77%), Postives = 77/137 (56.20%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRL---------DITGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  +AFV +G NL L         D   +   E + F+ E  +V+L++ +ILNGVCV W G I+L+ L+G   +E+D++ AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEVAFVATGTNLSLQFFPANLHGDQRQVPTREYVDFERETGKVYLKAPMILNGVCVIWRGWIDLQRLDGMGYLEYDQERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Ensembl Medaka
Match: cbfb (core-binding factor subunit beta [Source:NCBI gene;Acc:101157051])

HSP 1 Score: 87.0409 bits (214), Expect = 2.508e-20
Identity = 49/137 (35.77%), Postives = 77/137 (56.20%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCK--SGENAKNNFMQDLQSGKLNIAFVDSGINLRL---------DITGLSDNENIRFDDEE-RVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESV 591
            MP+ V DQ  KF+ +E F++LS +  I+YTG +    E  +  F    + G+  +AFV +G NL L         D   +   E + F+ E  +V+L++ +ILNGVCV W G I+L+ L+G   +E+D++ AQ E  
Sbjct:    1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEVAFVATGTNLSLQFFPANLHGDQRQVPTREYVDFERETGKVYLKAPMILNGVCVIWRGWIDLQRLDGMGYLEYDQERAQHEDA 137

[Close]

BLAST of Protein big brother vs. Planmine SMEST
Match: SMESG000068819.1 (SMESG000068819.1)

HSP 1 Score: 304.294 bits (778), Expect = 8.922e-106
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 1

Query:  217 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNKIE 660
            MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNKIE
Sbjct:    1 MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSGENAKNNFMQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNKIE 148

[Close]

BLAST of Protein big brother vs. Planmine SMEST
Match: SMESG000068819.1 (SMESG000068819.1)

HSP 1 Score: 217.624 bits (553), Expect = 3.161e-72
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 1

Query:  343 MQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNKIE 660
            MQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNKIE
Sbjct:    1 MQDLQSGKLNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLGHINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNKIE 106

The following BLAST results are available for this feature:

BLAST of Protein big brother vs. Ensembl Human
BLAST of Protein big brother vs. Ensembl Celegans
BLAST of Protein big brother vs. Ensembl Fly
BLAST of Protein big brother vs. Ensembl Zebrafish
BLAST of Protein big brother vs. Ensembl Xenopus
BLAST of Protein big brother vs. Ensembl Mouse
BLAST of Protein big brother vs. UniProt/SwissProt
BLAST of Protein big brother vs. TrEMBL
BLAST of Protein big brother vs. Ensembl Cavefish
BLAST of Protein big brother vs. Ensembl Sea Lamprey
BLAST of Protein big brother vs. Ensembl Yeast
BLAST of Protein big brother vs. Ensembl Nematostella
BLAST of Protein big brother vs. Ensembl Medaka
BLAST of Protein big brother vs. Planmine SMEST

BLAST of Protein big brother vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4


Match Name	E-value	Identity	Description
CBFB	8.159e-20	37.78	core-binding factor subunit beta [Source:HGNC Symb...	[more]
CBFB	1.002e-19	37.78	core-binding factor subunit beta [Source:HGNC Symb...	[more]
CBFB	2.607e-19	37.23	core-binding factor subunit beta [Source:HGNC Symb...	[more]
CBFB	4.194e-13	32.00	core-binding factor subunit beta [Source:HGNC Symb...	[more]

BLAST of Protein big brother vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
bro-1	8.706e-6	25.00	BROther (Drosophila tx factor partner) homolog [S...	[more]

BLAST of Protein big brother vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Bro	1.137e-25	37.84	gene:FBgn0013755 transcript:FBtr0072760	[more]
Bgb	6.139e-24	35.80	gene:FBgn0013753 transcript:FBtr0072758	[more]
Bgb	1.663e-23	35.80	gene:FBgn0013753 transcript:FBtr0330180	[more]

BLAST of Protein big brother vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cbfb	5.965e-19	35.77	core-binding factor subunit beta [Source:ZFIN;Acc:...	[more]
cbfb	7.736e-19	35.77	core-binding factor subunit beta [Source:ZFIN;Acc:...	[more]
cbfb	8.071e-19	35.77	core-binding factor subunit beta [Source:ZFIN;Acc:...	[more]
cbfb	8.071e-19	35.77	core-binding factor subunit beta [Source:ZFIN;Acc:...	[more]
cbfb	1.214e-18	35.77	core-binding factor subunit beta [Source:ZFIN;Acc:...	[more]

BLAST of Protein big brother vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
or1s1	1.231e-18	35.77	olfactory receptor family 1 subfamily S member 1 (...	[more]

BLAST of Protein big brother vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4


Match Name	E-value	Identity	Description
Cbfb	8.111e-20	37.78	core binding factor beta [Source:MGI Symbol;Acc:MG...	[more]
Cbfb	2.067e-19	37.23	core binding factor beta [Source:MGI Symbol;Acc:MG...	[more]
Cbfb	3.904e-19	37.59	core binding factor beta [Source:MGI Symbol;Acc:MG...	[more]
Cbfb	1.195e-12	31.50	core binding factor beta [Source:MGI Symbol;Acc:MG...	[more]

BLAST of Protein big brother vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4


Match Name	E-value	Identity	Description
sp\|Q24039\|BRO_DROME	1.140e-24	37.84	Protein brother OS=Drosophila melanogaster OX=7227...	[more]
sp\|Q24040\|BGB_DROME	6.154e-23	35.80	Protein big brother OS=Drosophila melanogaster OX=...	[more]
sp\|Q13951\|PEBB_HUMAN	4.811e-19	37.78	Core-binding factor subunit beta OS=Homo sapiens O...	[more]
sp\|Q08024\|PEBB_MOUSE	1.446e-18	37.23	Core-binding factor subunit beta OS=Mus musculus O...	[more]

BLAST of Protein big brother vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
E2BIL2	1.228e-23	40.94	Protein big brother OS=Harpegnathos saltator OX=61...	[more]
A0A158NKA4	4.783e-23	40.27	Uncharacterized protein OS=Atta cephalotes OX=1295...	[more]
A0A151WIS2	4.783e-23	40.27	Protein big brother OS=Trachymyrmex zeteki OX=6479...	[more]
A0A195EIT1	4.783e-23	40.27	Protein big brother OS=Trachymyrmex cornetzi OX=47...	[more]
A0A195BAT4	4.783e-23	40.27	Protein big brother OS=Atta colombica OX=520822 GN...	[more]

BLAST of Protein big brother vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
cbfb	1.247e-19	35.77	core-binding factor subunit beta [Source:NCBI gene...	[more]
cbfb	2.798e-19	35.77	core-binding factor subunit beta [Source:NCBI gene...	[more]

BLAST of Protein big brother vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000000610.1	8.006e-7	46.51	pep scaffold:Pmarinus_7.0:GL478173:5823:25505:-1 g...	[more]

BLAST of Protein big brother vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Protein big brother vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
CBFBETA	4.449e-22	38.46	Core-binding factor subunit beta; Predicted protei...	[more]

BLAST of Protein big brother vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
cbfb	1.477e-20	35.77	core-binding factor subunit beta [Source:NCBI gene...	[more]
cbfb	2.508e-20	35.77	core-binding factor subunit beta [Source:NCBI gene...	[more]

BLAST of Protein big brother vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000068819.1	8.922e-106	100.00	SMESG000068819.1	[more]
SMESG000068819.1	3.161e-72	100.00	SMESG000068819.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30016591 ID=SMED30016591|Name=Protein big brother|organism=Schmidtea mediterranea sexual|type=transcript|length=870bp
TTCAAATTCTATAATTATTACAAAAGAATATTGTAGTATGATAATGTGAT
TACTCATCGATACTGGCGCAAATTCGGTTAGACAGACAGAAATCCAATTT
GTAGTTATTCATACTTGAATTAATCATGCTCTGCATGCCTTATCAAAATA
AAAGATACAACTAGATATAATCTACTGCATTGATTCACTTATTTGTGTCC
TGATTTTCACCTCAAAATGCCGAAGACAGTTTTGGATCAAGAAGGAAAGT
TTAAGCAAGATGAAATTTTTAAAAGACTTTCAGAAGATAATAGAATTCGT
TATACAGGTTGCAAATCGGGTGAAAATGCGAAAAACAATTTCATGCAAGA
CCTACAATCAGGAAAACTAAATATAGCATTTGTGGATTCTGGAATCAACT
TGAGGCTGGACATCACCGGTTTATCGGATAATGAGAATATAAGATTCGAT
GATGAAGAACGTGTATTCCTTCAGTCACATTTGATTTTAAATGGCGTTTG
TGTAAAATGGCTTGGTCATATTAATCTTAAAGACTTAGAAGGAAGTGCTC
GAATTGAATTTGATCGGAAAATGGCTCAAGAGGAATCAGTATCGTGTTGG
AACCAATTATACGATTATAATTTCCATTTCGGATTCACAGAGGATAACAA
TAAAATCGAGTAACATTAGGAAAGTACAGTCAACAAAAACTGTTACATTT
AAATTACTATTTCTGTCCGTTTGCGCATTTATAACCGATCCAGATGTAGA
AATTTGATATTTGGATAAATTACTGTATTTGCTGTATACAATTTCTATCT
ATTTATTTTTGATAATTATATAATTGTTATATAAACCATTTTGTAATTTA
ATACCGATGTTTTTTAAAAA

protein sequence of SMED30016591-orf-1

>SMED30016591-orf-1 ID=SMED30016591-orf-1|Name=SMED30016591-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=149bp
MPKTVLDQEGKFKQDEIFKRLSEDNRIRYTGCKSGENAKNNFMQDLQSGK
LNIAFVDSGINLRLDITGLSDNENIRFDDEERVFLQSHLILNGVCVKWLG
HINLKDLEGSARIEFDRKMAQEESVSCWNQLYDYNFHFGFTEDNNKIE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:1903508	positive regulation of nucleic acid-templated transcription
GO:1903506	regulation of nucleic acid-templated transcription

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000101	muscle cell
PLANA:0000429	neoblast
PLANA:0002032	epidermal cell
PLANA:0002075	pigment cell
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036552	CBF_bsu_sf
IPR003417	CBF_beta

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **molecular function**
Term	Definition
GO:0003713	transcription coactivator activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036552	Core-binding factor, beta subunit superfamily	GENE3D	G3DSA:2.40.250.10		coord: 1..128 e-value: 7.3E-36 score: 125.1
IPR036552	Core-binding factor, beta subunit superfamily	SUPERFAMILY	SSF50723	Core binding factor beta, CBF	coord: 5..124
IPR003417	Core-binding factor, beta subunit	PFAM	PF02312	CBF_beta	coord: 1..144 e-value: 1.3E-34 score: 119.3
IPR003417	Core-binding factor, beta subunit	PANTHER	PTHR10276	CORE-BINDING FACTOR, BETA SUBUNIT	coord: 1..145

Main menu

Protein big brother

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 15

Owlery

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