39S ribosomal protein L14, mitochondrial

Overview
Name39S ribosomal protein L14, mitochondrial
Smed IDSMED30016559
Length (bp)621
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 39S ribosomal protein L14, mitochondrial (SMED30016559) t-SNE clustered cells

Violin plots show distribution of expression levels for 39S ribosomal protein L14, mitochondrial (SMED30016559) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 39S ribosomal protein L14, mitochondrial (SMED30016559) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 39S ribosomal protein L14, mitochondrial (SMED30016559) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016559

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30016559h1SMcG0004876 SmedASXL_019234SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Human
Match: MRPL14 (mitochondrial ribosomal protein L14 [Source:HGNC Symbol;Acc:HGNC:14279])

HSP 1 Score: 102.064 bits (253), Expect = 4.137e-27
Identity = 56/121 (46.28%), Postives = 78/121 (64.46%), Query Frame = 3 
Query:  204 GSIWLLSKMT---IVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPGV-PRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            GS+  + KMT   +VDNS +G       +  + IHVY K   G +GD++LL ++G+ KK  IVG     P + PRFDSNNVVL+ ++GNP+GTRI  PIP+ LR   G+ +K+LAIA  F+
Sbjct:   27 GSLSAIQKMTRVRVVDNSALGNSP--YHRAPRCIHVYKKNGVGKVGDQILLAIKGQKKKALIVGHCMPGPRMTPRFDSNNVVLIEDNGNPVGTRIKTPIPTSLRKREGEYSKVLAIAQNFV 145
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Celegans
Match: mrpl-14 (Mitochondrial Ribosomal Protein, Large [Source:UniProtKB/TrEMBL;Acc:Q20430])

HSP 1 Score: 102.449 bits (254), Expect = 2.879e-27
Identity = 55/123 (44.72%), Postives = 77/123 (62.60%), Query Frame = 3 
Query:  222 SKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRG------NLGDKVLLTVRGEMKKGYIVGLRQH----EPGVPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDI--NKILAIATKFI 554
            +++ +VDNS +GKEA   GK    IHVY +  R        LGDK+L+ +RG+M+K Y+VG   H    + GVP  D N +VL+ E+GNPLG RI  PIP+ L    G++  +K+LA+A KFI
Sbjct:   57 TRLNVVDNSALGKEADTTGKLPYCIHVYKQGRRAKHMPHAELGDKILVAIRGQMRKAYVVGANTHVHYRKHGVPVTDQNMIVLLDEEGNPLGNRITAPIPTKLMANRGNVQFSKVLALANKFI 179
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Fly
Match: mRpL14 (gene:FBgn0040389 transcript:FBtr0333730)

HSP 1 Score: 123.635 bits (309), Expect = 1.399e-35
Identity = 60/125 (48.00%), Postives = 92/125 (73.60%), Query Frame = 3 
Query:  207 SIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHE-PGVPRFDSNNVVLVAEDGNPLGTRILVPIPSMLR--------GYPGDINKILAIATKFI 554
             I  L+++ +VDNS++GK+A   G+P + IHVYNK+  G +GDKVL+ ++G+MKKG +VGL+Q++ P  P+FDSNN+VL+ ++G+PLGTRI VPIP++LR            D  K+LAIA++++
Sbjct:   37 EIRKLARLRVVDNSDLGKKAMAEGRPPRCIHVYNKRGVGFIGDKVLVAIKGQMKKGILVGLKQNQKPKQPKFDSNNLVLIDDNGSPLGTRIHVPIPTILRTILKEKTLAKGADYTKVLAIASRYV 161
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Fly
Match: mRpL14 (gene:FBgn0040389 transcript:FBtr0070405)

HSP 1 Score: 123.635 bits (309), Expect = 1.399e-35
Identity = 60/125 (48.00%), Postives = 92/125 (73.60%), Query Frame = 3 
Query:  207 SIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHE-PGVPRFDSNNVVLVAEDGNPLGTRILVPIPSMLR--------GYPGDINKILAIATKFI 554
             I  L+++ +VDNS++GK+A   G+P + IHVYNK+  G +GDKVL+ ++G+MKKG +VGL+Q++ P  P+FDSNN+VL+ ++G+PLGTRI VPIP++LR            D  K+LAIA++++
Sbjct:   37 EIRKLARLRVVDNSDLGKKAMAEGRPPRCIHVYNKRGVGFIGDKVLVAIKGQMKKGILVGLKQNQKPKQPKFDSNNLVLIDDNGSPLGTRIHVPIPTILRTILKEKTLAKGADYTKVLAIASRYV 161
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Zebrafish
Match: mrpl14 (mitochondrial ribosomal protein L14 [Source:NCBI gene;Acc:394122])

HSP 1 Score: 109.768 bits (273), Expect = 2.170e-30
Identity = 62/133 (46.62%), Postives = 88/133 (66.17%), Query Frame = 3 
Query:  165 RSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG---VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            R+ S S  ++    +I  L+++ +VDNS +G   A   +P KVIHVYNK   G +GD+VLL ++G+ KK  IVG +   PG    PRFDSNNVVL+ ++GNP GTRI  P+P+ LR   G+ +K+LAIA +F+
Sbjct:   17 RAFSVSSAVS----AIQKLTRVRVVDNSTLGN--AHHHRPPKVIHVYNKNGVGKVGDRVLLAIKGQKKKAIIVGHKM--PGARMTPRFDSNNVVLIEDNGNPTGTRIKAPLPTHLRKLEGEYSKLLAIAQRFV 141
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Xenopus
Match: fat4 (FAT atypical cadherin 4 [Source:Xenbase;Acc:XB-GENE-981052])

HSP 1 Score: 113.235 bits (282), Expect = 7.896e-32
Identity = 59/125 (47.20%), Postives = 85/125 (68.00%), Query Frame = 3 
Query:  189 LNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG---VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            L+   G+I  L+++ +VDNS +G  +    +P K IHVYNK   G +GD++LL ++G+ KK  IVG +   PG    PRFDSNNVVL+ ++GNP+GTRI  PIP+ LR + G+ +K+LAIA  F+
Sbjct:    2 LSAPCGAIQKLTRVRVVDNSTLG--STPYHRPPKCIHVYNKSGVGKVGDRILLAIKGQKKKALIVGHKM--PGASMTPRFDSNNVVLIEDNGNPVGTRIKTPIPTSLRRHDGEFSKVLAIAQNFV 122
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Mouse
Match: Mrpl14 (mitochondrial ribosomal protein L14 [Source:MGI Symbol;Acc:MGI:1333864])

HSP 1 Score: 112.464 bits (280), Expect = 2.469e-31
Identity = 65/139 (46.76%), Postives = 88/139 (63.31%), Query Frame = 3 
Query:  156 FHFRSLSTSVVLNKKYGSIWLLSKMT---IVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG---VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            F  R  STS       GS+  + KMT   +VDNS +G  +    +P + IHVYNK   G +GD++LL +RG+ KK  IVG R   PG    P+FDSNNVVL+ ++GNP+GTRI +PIP+ LR   G+ +K+LAIA  F+
Sbjct:   18 FSQRCFSTS-------GSLSAVQKMTRVRVVDNSALG--STPYHRPPRCIHVYNKSGVGKVGDQILLAIRGQKKKALIVGHRM--PGSRMTPKFDSNNVVLIEDNGNPVGTRIKIPIPTSLRRREGEYSKVLAIAQNFV 145
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. UniProt/SwissProt
Match: sp|Q1JQ99|RM14_BOVIN (39S ribosomal protein L14, mitochondrial OS=Bos taurus OX=9913 GN=MRPL14 PE=1 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.610e-31
Identity = 61/134 (45.52%), Postives = 87/134 (64.93%), Query Frame = 3 
Query:  156 FHFRSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPGV-PRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            F  R  ST+  L    G+I  ++++ +VDNS +G       +P + IHVYNK   G +GD++LL ++G+ KK  IVG R   P + PRFDSNNVVL+ ++GNP+GTRI  PIP+ LR   G+ +K+LAIA  F+
Sbjct:   18 FSQRCFSTTGSL----GAIQKMTRVRVVDNSALGNTP--YHRPPRCIHVYNKNGVGKVGDRILLAIKGQKKKALIVGHRMPGPTMTPRFDSNNVVLIEDNGNPVGTRIKTPIPTSLRQREGEFSKVLAIAQNFV 145
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. UniProt/SwissProt
Match: sp|Q9D1I6|RM14_MOUSE (39S ribosomal protein L14, mitochondrial OS=Mus musculus OX=10090 GN=Mrpl14 PE=1 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.727e-30
Identity = 65/139 (46.76%), Postives = 88/139 (63.31%), Query Frame = 3 
Query:  156 FHFRSLSTSVVLNKKYGSIWLLSKMT---IVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG---VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            F  R  STS       GS+  + KMT   +VDNS +G  +    +P + IHVYNK   G +GD++LL +RG+ KK  IVG R   PG    P+FDSNNVVL+ ++GNP+GTRI +PIP+ LR   G+ +K+LAIA  F+
Sbjct:   18 FSQRCFSTS-------GSLSAVQKMTRVRVVDNSALG--STPYHRPPRCIHVYNKSGVGKVGDQILLAIRGQKKKALIVGHRM--PGSRMTPKFDSNNVVLIEDNGNPVGTRIKIPIPTSLRRREGEYSKVLAIAQNFV 145
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. UniProt/SwissProt
Match: sp|Q7M0E7|RM14_RAT (39S ribosomal protein L14, mitochondrial OS=Rattus norvegicus OX=10116 GN=Mrpl14 PE=1 SV=2)

HSP 1 Score: 110.923 bits (276), Expect = 5.974e-30
Identity = 64/136 (47.06%), Postives = 86/136 (63.24%), Query Frame = 3 
Query:  165 RSLSTSVVLNKKYGSIWLLSKMT---IVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG---VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            R  STS       GS+  + KMT   +VDNS +G       +P + IHVYNK   G +GD++LL +RG+ KK  IVG R   PG    P+FDSNNVVL+ ++GNP+GTRI +PIP+ LR   G+ +K+LAIA  F+
Sbjct:   21 RCFSTS-------GSLSAIQKMTRVRVVDNSALGNTP--YHRPPRCIHVYNKSGVGKVGDQILLAIRGQKKKALIVGHRM--PGSRMTPKFDSNNVVLIEDNGNPVGTRIKIPIPTSLRRREGEYSKVLAIAQNFV 145
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. UniProt/SwissProt
Match: sp|Q7ZUH5|RM14_DANRE (39S ribosomal protein L14, mitochondrial OS=Danio rerio OX=7955 GN=mrpl14 PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.638e-29
Identity = 62/133 (46.62%), Postives = 88/133 (66.17%), Query Frame = 3 
Query:  165 RSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG---VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            R+ S S  ++    +I  L+++ +VDNS +G   A   +P KVIHVYNK   G +GD+VLL ++G+ KK  IVG +   PG    PRFDSNNVVL+ ++GNP GTRI  P+P+ LR   G+ +K+LAIA +F+
Sbjct:   17 RAFSVSSAVS----AIQKLTRVRVVDNSTLGN--AHHHRPPKVIHVYNKNGVGKVGDRVLLAIKGQKKKAIIVGHKM--PGARMTPRFDSNNVVLIEDNGNPTGTRIKAPLPTHLRKLEGEYSKLLAIAQRFV 141
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. UniProt/SwissProt
Match: sp|Q6P1L8|RM14_HUMAN (39S ribosomal protein L14, mitochondrial OS=Homo sapiens OX=9606 GN=MRPL14 PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.987e-26
Identity = 56/121 (46.28%), Postives = 78/121 (64.46%), Query Frame = 3 
Query:  204 GSIWLLSKMT---IVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPGV-PRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            GS+  + KMT   +VDNS +G       +  + IHVY K   G +GD++LL ++G+ KK  IVG     P + PRFDSNNVVL+ ++GNP+GTRI  PIP+ LR   G+ +K+LAIA  F+
Sbjct:   27 GSLSAIQKMTRVRVVDNSALGNSP--YHRAPRCIHVYKKNGVGKVGDQILLAIKGQKKKALIVGHCMPGPRMTPRFDSNNVVLIEDNGNPVGTRIKTPIPTSLRKREGEYSKVLAIAQNFV 145
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. TrEMBL
Match: A0A0B6YXP9 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF38986 PE=3 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 3.718e-46
Identity = 82/140 (58.57%), Postives = 105/140 (75.00%), Query Frame = 3 
Query:  138 IFKNINFHFRSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQ-HEPGVPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            + + I     S STS  L++    I LL++M IVDNS +GK AA +GKP +VIHVYN++  G LGDKVL+TV+GEMK+ YIVGLRQ  +P VPR D+NN+VLV ++GNPLG RI VPIPS LRG  GD+ KIL++ATKF+
Sbjct:   12 VIQYIGTSCCSFSTSQNLHE----IRLLARMRIVDNSAIGKAAAQIGKPPRVIHVYNRRRVGYLGDKVLVTVKGEMKRAYIVGLRQKQKPNVPRMDTNNIVLVDDNGNPLGNRITVPIPSALRGRGGDLTKILSLATKFV 147
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. TrEMBL
Match: A0A2C9KVT4 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106056930 PE=3 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 4.496e-46
Identity = 80/130 (61.54%), Postives = 100/130 (76.92%), Query Frame = 3 
Query:  168 SLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHE-PGVPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
              STS  L++    +  L +M IVDNS +GK AA +GKP KVIHVYNK+  G LGDKVL+TV+GEMKK YIVGLRQ + P VPR D+NN+VLV + G+PLGTR++VPIPS LRG  GD+ KIL++ATKF+
Sbjct:   26 QFSTSQTLHE----VRKLMRMRIVDNSAIGKAAAQIGKPPKVIHVYNKRQVGFLGDKVLVTVKGEMKKAYIVGLRQKQLPNVPRMDTNNIVLVDDKGDPLGTRVMVPIPSALRGKGGDLTKILSLATKFV 151
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. TrEMBL
Match: A0A3S1A0K8 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_012602 PE=3 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.154e-42
Identity = 74/122 (60.66%), Postives = 97/122 (79.51%), Query Frame = 3 
Query:  192 NKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG-VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            +K +  I  L++M IVDNS +GK AA +GKP +VI+VYN+++ G LGDKVL+TV+GEM++ YIVGLRQ +   VPR D+NN+VLV ++GNPLG RI VPIPS LRG  GDI KIL++ATKF+
Sbjct:   29 SKTHYEIRNLARMRIVDNSAIGKAAAEIGKPPRVINVYNRKNVGYLGDKVLVTVKGEMRRAYIVGLRQKQKANVPRMDTNNIVLVDDNGNPLGNRIHVPIPSCLRGKGGDITKILSLATKFV 150
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. TrEMBL
Match: T1F6R9 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20204518 PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 1.099e-41
Identity = 74/131 (56.49%), Postives = 99/131 (75.57%), Query Frame = 3 
Query:  165 RSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG-VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            R  ST+ V ++    +  L+++ +VDNS +G+ A L GKP ++IHVYNK   G LGDKVLL ++G+ K+GY+VGL+Q++   VP++DSNNVVLV ++GNP GTRI VPIPS LRG  GDI+KILA ATKFI
Sbjct:   12 RYFSTTAVRHE----VQKLTRLRVVDNSLIGRAAELAGKPGRIIHVYNKTGIGKLGDKVLLAIQGQKKRGYLVGLKQNQDRMVPKYDSNNVVLVEDNGNPTGTRIRVPIPSCLRGKKGDISKILACATKFI 138
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. TrEMBL
Match: A0A2T7P050 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_14467 PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.901e-40
Identity = 69/131 (52.67%), Postives = 97/131 (74.05%), Query Frame = 3 
Query:  165 RSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQ-HEPGVPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            +S ST+  L +    +  L+++ +VDNS +G+ A  +GKPA++IHVYNK+  G LGDKVLL ++G+ K  YIVGL+Q  +P +PRFDSNNVVLV E+G P+GTR+  PIPS+LR   GD  KIL++AT+F+
Sbjct:   18 QSFSTTQCLQE----LRKLARVRVVDNSALGRAAVAIGKPARIIHVYNKREVGYLGDKVLLAIKGQKKHAYIVGLKQKQKPNIPRFDSNNVVLVEENGTPVGTRLTAPIPSVLRNKEGDFTKILSLATRFV 144
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Cavefish
Match: mrpl14 (mitochondrial ribosomal protein L14 [Source:NCBI gene;Acc:103025862])

HSP 1 Score: 105.145 bits (261), Expect = 1.516e-28
Identity = 59/118 (50.00%), Postives = 78/118 (66.10%), Query Frame = 3 
Query:  210 IWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG---VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            I  L+++ +VDNS +G       +P KVIHVYNK   G +GD VLL ++G+ KK  IVG +   PG     RFDSNNVVL+ ++GNP GTRI  PIP+ LR   GD +K+LAIA +F+
Sbjct:   32 IQKLTRVRVVDNSTLGNTP--YHRPPKVIHVYNKNGVGKVGDVVLLAIKGQKKKALIVGHKM--PGKRMTARFDSNNVVLIEDNGNPTGTRIKAPIPTHLRRLEGDYSKLLAIAQRFV 145
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Sea Lamprey
Match: mrpl14 (mitochondrial ribosomal protein L14 [Source:NCBI gene;Acc:394122])

HSP 1 Score: 105.145 bits (261), Expect = 3.234e-29
Identity = 56/125 (44.80%), Postives = 82/125 (65.60%), Query Frame = 3 
Query:  186 VLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPGV--PRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            +++    +I  ++++ +VDNS +  +     +P +VIHVY K   G +GD VLL +RGE KK  IVG +    GV  PRFDSNNVVL+ ++G+P GTRI VPIP+ LR      +K+LAIA++F+
Sbjct:   27 LMSPASAAIQKMTRVRVVDNSPMATKP--YHRPPRVIHVYTKNSVGKVGDMVLLAIRGEKKKALIVGHKMSS-GVMNPRFDSNNVVLLEDNGSPTGTRIRVPIPTSLRSSERQYSKVLAIASRFV 148
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Nematostella
Match: EDO46559 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPT1])

HSP 1 Score: 81.6481 bits (200), Expect = 2.677e-20
Identity = 45/113 (39.82%), Postives = 70/113 (61.95%), Query Frame = 3 
Query:  219 LSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQ-HEPGVPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            L++  +VDNS +G+      KP  +I  Y K+    +GD + + V G+  K  +VG R+     VPR+D+NN+VLV ++  PLGTRI  PIP+++R      +K+LAIA++FI
Sbjct:    1 LTRFNVVDNSALGQRTKRNRKPY-LIGFYRKRKTAEVGDVIRVAVGGKTNKAIVVGTRKPKHHSVPRYDNNNIVLVDDNLAPLGTRIKGPIPTIIRRRQAKYSKVLAIASRFI 112
[Close]
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Medaka
Match: mrpl14 (mitochondrial ribosomal protein L14 [Source:NCBI gene;Acc:101164087])

HSP 1 Score: 115.161 bits (287), Expect = 7.444e-32
Identity = 67/148 (45.27%), Postives = 92/148 (62.16%), Query Frame = 3 
Query:  120 SFCKLDIFKNINFHFRSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPG---VPRFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI 554
            SF  L +  +     R+ S SVV      +I  ++++ +VDNS +G       +P +VIHVY K   G +GDKVLL ++G+ KK  IVG +   PG    PRFDSNNVVL+ ++GNP GTRI VPIP+ LR   GD +K+LAIA+ F+
Sbjct:   62 SFSGLIVQNSSKILQRAFSVSVVA----AAIQKMTRVRVVDNSSLGNTP--YHRPPRVIHVYTKNGVGKVGDKVLLAIKGQKKKALIVGHKM--PGDRMTPRFDSNNVVLIEDNGNPTGTRIKVPIPTHLRQMEGDFSKVLAIASSFV 201
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Planmine SMEST
Match: SMESG000005180.1 (SMESG000005180.1)

HSP 1 Score: 262.692 bits (670), Expect = 6.591e-91
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 3 
Query:  114 MRSFCKLDIFKNINFHFRSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPGVPRFDSNNVVLVAEDGNPLGTRILVPIPSM 497
            MRSFCKLDIFKNINFHFRSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPGVPRFDSNNVVLVAEDGNPLGTRILVPIPSM
Sbjct:    1 MRSFCKLDIFKNINFHFRSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKEAALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPGVPRFDSNNVVLVAEDGNPLGTRILVPIPSM 128
The following BLAST results are available for this feature:
BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPL144.137e-2746.28mitochondrial ribosomal protein L14 [Source:HGNC S... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl-142.879e-2744.72Mitochondrial Ribosomal Protein, Large [Source:Un... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mRpL141.399e-3548.00gene:FBgn0040389 transcript:FBtr0333730[more]
mRpL141.399e-3548.00gene:FBgn0040389 transcript:FBtr0070405[more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl142.170e-3046.62mitochondrial ribosomal protein L14 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fat47.896e-3247.20FAT atypical cadherin 4 [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Mrpl142.469e-3146.76mitochondrial ribosomal protein L14 [Source:MGI Sy... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q1JQ99|RM14_BOVIN3.610e-3145.5239S ribosomal protein L14, mitochondrial OS=Bos ta... [more]
sp|Q9D1I6|RM14_MOUSE1.727e-3046.7639S ribosomal protein L14, mitochondrial OS=Mus mu... [more]
sp|Q7M0E7|RM14_RAT5.974e-3047.0639S ribosomal protein L14, mitochondrial OS=Rattus... [more]
sp|Q7ZUH5|RM14_DANRE1.638e-2946.6239S ribosomal protein L14, mitochondrial OS=Danio ... [more]
sp|Q6P1L8|RM14_HUMAN1.987e-2646.2839S ribosomal protein L14, mitochondrial OS=Homo s... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B6YXP93.718e-4658.57Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A2C9KVT44.496e-4661.54Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A3S1A0K81.154e-4260.66Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
T1F6R91.099e-4156.49Uncharacterized protein OS=Helobdella robusta OX=6... [more]
A0A2T7P0502.901e-4052.67Uncharacterized protein OS=Pomacea canaliculata OX... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl141.516e-2850.00mitochondrial ribosomal protein L14 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl143.234e-2944.80mitochondrial ribosomal protein L14 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO465592.677e-2039.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl147.444e-3245.27mitochondrial ribosomal protein L14 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L14, mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000005180.16.591e-91100.00SMESG000005180.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016559 ID=SMED30016559|Name=39S ribosomal protein L14, mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=621bp
TTTTTTTAAATTTTGTATTTTGTCCCTCTGTTAAATTATCATTAATAATA
ACTTTTTATTTGTTAATAAAATAAGTTATATAAAGACTGTATACTGTATA
TAACTTTAACACAATGCGTTCATTCTGTAAACTTGATATATTTAAAAACA
TAAATTTTCATTTTCGTTCTTTAAGTACTTCAGTGGTATTGAATAAAAAG
TATGGATCGATATGGTTGCTTTCAAAAATGACAATTGTTGACAACAGTGA
AGTTGGTAAAGAAGCTGCTTTAGTTGGAAAGCCAGCGAAAGTAATTCACG
TTTATAATAAACAGCATCGTGGAAATCTTGGAGATAAAGTACTTCTAACA
GTCAGAGGAGAAATGAAAAAAGGATATATAGTAGGTCTTCGACAACATGA
ACCAGGAGTACCACGTTTTGATTCTAATAATGTGGTTTTGGTTGCTGAAG
ATGGTAATCCACTTGGAACACGCATTTTAGTACCAATCCCATCTATGTTA
CGAGGTTATCCTGGCGATATCAACAAAATTTTGGCTATTGCTACGAAATT
TATTTAAGTTTTTATTTTTAAAATGTATAAATAAATTGCAATCATTGAAC
ATCAGAAGGGCTAATCATCGA
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protein sequence of SMED30016559-orf-1

>SMED30016559-orf-1 ID=SMED30016559-orf-1|Name=SMED30016559-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=148bp
MRSFCKLDIFKNINFHFRSLSTSVVLNKKYGSIWLLSKMTIVDNSEVGKE
AALVGKPAKVIHVYNKQHRGNLGDKVLLTVRGEMKKGYIVGLRQHEPGVP
RFDSNNVVLVAEDGNPLGTRILVPIPSMLRGYPGDINKILAIATKFI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR000218Ribosomal_L14P
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation