SMED30016539

Overview
NameSMED30016539
Smed IDSMED30016539
Length (bp)554
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016539 (SMED30016539) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016539 (SMED30016539) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016539 (SMED30016539) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016539 (SMED30016539) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30016539

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 48

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30016539h1SMcG0014569 Contig3842uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539h1SMcG0014569 Contig3842newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539h1SMcG0020710 Contig3842uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539h1SMcG0020710 Contig3842newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539 Contig3109GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539h1SMcG0014569 Contig3842uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539h1SMcG0014569 Contig3842newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539h1SMcG0020710 Contig3842uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539h1SMcG0020710 Contig3842newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30016539 Contig3109GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016539h1SMcG0020697 Contig5979newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016539h1SMcG0020697 Contig5979uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016539h1SMcG0020697 Contig5979newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016539h1SMcG0020697 Contig5979uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30016539h1SMcG0020699 dd_Smed_v6_1245_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30016539h1SMcG0020697 dd_Smed_v6_1245_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30016539h1SMcG0020699 dd_Smed_v6_1245_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30016539h1SMcG0020697 dd_Smed_v6_1245_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020697 dd_Smed_v4_8920_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020710 dd_Smed_v4_2030_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020697 dd_Smed_v4_8920_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016539h1SMcG0020697 dd_Smed_v4_639_2_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016539 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002264:46650..47891 -1757
v31.001800:31369..32630 -1707
v31.041493:3655..4408 -1387
Homology
BLAST of SMED30016539 vs. Ensembl Human
Match: ANKRD28 (ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:HGNC:29024])

HSP 1 Score: 88.9669 bits (219), Expect = 2.313e-20
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+VKLL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +V+ L++   D+N  +     PL +A  N
Sbjct:   85 RTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYN 193          

HSP 2 Score: 62.3882 bits (150), Expect = 4.358e-11
Identity = 37/131 (28.24%), Postives = 58/131 (44.27%), Query Frame = 3
Query:  231 FAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            F  + E V++L++H+ D+ A DK  Q P+H+A                                  + GH E VKLL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   27 FLFYLEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAA 157          

HSP 3 Score: 53.5286 bits (127), Expect = 4.723e-8
Identity = 31/117 (26.50%), Postives = 61/117 (52.14%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   E +  ++ +D     PL +++ N + + V
Sbjct:  497 PLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCV 613          

HSP 4 Score: 49.6766 bits (117), Expect = 9.683e-7
Identity = 37/144 (25.69%), Postives = 60/144 (41.67%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+    +   +++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  114 DKKD---RRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGAIVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPL 254          

HSP 5 Score: 47.7506 bits (112), Expect = 4.362e-6
Identity = 34/115 (29.57%), Postives = 57/115 (49.57%), Query Frame = 3
Query:  192 KDVDLRR-PIHHACFAGHAEIVKLLI---EHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD  L+R PIH A   GH+E ++LLI   E    ++  D   Q P+ L+   GH++ V  L+    +++A DK     +H    +G+ E V+ L++       RD     P+ ++
Sbjct:  556 KDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLS 670          
BLAST of SMED30016539 vs. Ensembl Human
Match: ANKRD28 (ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:HGNC:29024])

HSP 1 Score: 88.9669 bits (219), Expect = 2.811e-20
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+VKLL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +V+ L++   D+N  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYN 249          

HSP 2 Score: 68.5514 bits (166), Expect = 3.456e-13
Identity = 41/144 (28.47%), Postives = 63/144 (43.75%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E VKLL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   70 KDSKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAA 213          

HSP 3 Score: 58.151 bits (139), Expect = 1.362e-9
Identity = 38/146 (26.03%), Postives = 67/146 (45.89%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+ HN  + ++D   + P+ +A   G +  V++L+   + ++   D      +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  802 GAAEMLIDTLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSHNAQVNSVDSTGKTPLMMAAENGQTNTVEMLVSSASAELTLQDNSKNTALHLACSKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLG 947          

HSP 4 Score: 55.0694 bits (131), Expect = 1.218e-8
Identity = 37/129 (28.68%), Postives = 60/129 (46.51%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA----LDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V  L++H       D + + PIHL+   GH   +  L++    M+A     D      +HW C +G+   VELL+E++      +    +PL  A+ N+N+   E+L
Sbjct:  680 DAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPATADNHGYTALHWACYNGHETCVELLLEQEV-FQKTEGNAFSPLHCAVINDNEGAAEML 807          

HSP 5 Score: 53.1434 bits (126), Expect = 5.747e-8
Identity = 31/117 (26.50%), Postives = 61/117 (52.14%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   E +  ++ +D     PL +++ N + + V
Sbjct:  553 PLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCV 669          

HSP 6 Score: 49.2914 bits (116), Expect = 1.165e-6
Identity = 37/144 (25.69%), Postives = 60/144 (41.67%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+    +   +++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  170 DKKD---RRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGAIVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPL 310          

HSP 7 Score: 47.3654 bits (111), Expect = 5.241e-6
Identity = 34/115 (29.57%), Postives = 57/115 (49.57%), Query Frame = 3
Query:  192 KDVDLRR-PIHHACFAGHAEIVKLLI---EHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD  L+R PIH A   GH+E ++LLI   E    ++  D   Q P+ L+   GH++ V  L+    +++A DK     +H    +G+ E V+ L++       RD     P+ ++
Sbjct:  612 KDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLS 726          
BLAST of SMED30016539 vs. Ensembl Human
Match: ANKRD28 (ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:HGNC:29024])

HSP 1 Score: 88.9669 bits (219), Expect = 2.811e-20
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+VKLL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +V+ L++   D+N  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYN 249          

HSP 2 Score: 68.5514 bits (166), Expect = 3.456e-13
Identity = 41/144 (28.47%), Postives = 63/144 (43.75%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E VKLL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   70 KDSKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAA 213          

HSP 3 Score: 58.151 bits (139), Expect = 1.362e-9
Identity = 38/146 (26.03%), Postives = 67/146 (45.89%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+ HN  + ++D   + P+ +A   G +  V++L+   + ++   D      +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  802 GAAEMLIDTLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSHNAQVNSVDSTGKTPLMMAAENGQTNTVEMLVSSASAELTLQDNSKNTALHLACSKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLG 947          

HSP 4 Score: 55.0694 bits (131), Expect = 1.218e-8
Identity = 37/129 (28.68%), Postives = 60/129 (46.51%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA----LDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V  L++H       D + + PIHL+   GH   +  L++    M+A     D      +HW C +G+   VELL+E++      +    +PL  A+ N+N+   E+L
Sbjct:  680 DAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPATADNHGYTALHWACYNGHETCVELLLEQEV-FQKTEGNAFSPLHCAVINDNEGAAEML 807          

HSP 5 Score: 53.1434 bits (126), Expect = 5.747e-8
Identity = 31/117 (26.50%), Postives = 61/117 (52.14%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   E +  ++ +D     PL +++ N + + V
Sbjct:  553 PLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCV 669          

HSP 6 Score: 49.2914 bits (116), Expect = 1.165e-6
Identity = 37/144 (25.69%), Postives = 60/144 (41.67%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+    +   +++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  170 DKKD---RRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGAIVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPL 310          

HSP 7 Score: 47.3654 bits (111), Expect = 5.241e-6
Identity = 34/115 (29.57%), Postives = 57/115 (49.57%), Query Frame = 3
Query:  192 KDVDLRR-PIHHACFAGHAEIVKLLI---EHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD  L+R PIH A   GH+E ++LLI   E    ++  D   Q P+ L+   GH++ V  L+    +++A DK     +H    +G+ E V+ L++       RD     P+ ++
Sbjct:  612 KDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLS 726          
BLAST of SMED30016539 vs. Ensembl Human
Match: ANKRD50 (ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:HGNC:29223])

HSP 1 Score: 77.411 bits (189), Expect = 3.324e-16
Identity = 42/134 (31.34%), Postives = 77/134 (57.46%), Query Frame = 3
Query:  165 YFKEEFS--ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQPIHWEC---ESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            YF E  +  E  D + R  +H +C+ GH E+V++LI ++ D+ A D +K+  +  A +QGH + V+LLIEH   ++    Q     C   + G+I++V++L+E   D N  D+     + +A KN + +++++L
Sbjct:  964 YFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLL 1097          

HSP 2 Score: 58.9214 bits (141), Expect = 7.436e-10
Identity = 40/153 (26.14%), Postives = 77/153 (50.33%), Query Frame = 3
Query:  126 NAIRSGSVELFRRYFKEEFSER-----KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK------QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            NA+R  ++E  R   +  FS       KD D R  ++         + +  +E+  ++EA D + +  +H++C+QGH E V++LI ++ D+ A D       Q   W+   G++++V+LLIE    ++         L IA +  + ++V++L
Sbjct:  915 NALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQ---GHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVL 1064          

HSP 3 Score: 58.151 bits (139), Expect = 1.365e-9
Identity = 35/127 (27.56%), Postives = 63/127 (49.61%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--------ALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            D D R  +  A + GH +IV  L++H  ++   D + +  +  A Y GH E V+ L++H  ++         AL    +      G+  +V LLI+R  +++  DK  M PL +A    + ++V++L
Sbjct:  641 DADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLL 767          

HSP 4 Score: 56.225 bits (134), Expect = 5.007e-9
Identity = 41/135 (30.37%), Postives = 64/135 (47.41%), Query Frame = 3
Query:   96 SMDEEFKLFIN-AIRSGSVELFRRYFKEEFSE-RKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH--NCDMEALD-KQPIHWECESGYIEIVELLIERKCDINARD 485
            S+D E +  ++ A   G+VE+ R        E  +D     P+H A F GH  I + LIE       +D   + P  LA  +GH + V++L+E+  N D    D +  +      G+ +IVELL     D+N +D
Sbjct:  809 SIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKD 943          

HSP 5 Score: 52.7582 bits (125), Expect = 8.322e-8
Identity = 37/128 (28.91%), Postives = 60/128 (46.88%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFA-----GHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +DVD R  +  A        GHA +V LLI+   +++  DK    P+ +A Y+GH + V LL+E   D++  D   + P+      G+  +V  L+     +++ D      L IA    N E+V  L
Sbjct:  706 EDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTL 833          

HSP 6 Score: 48.1358 bits (113), Expect = 3.158e-6
Identity = 33/129 (25.58%), Postives = 62/129 (48.06%), Query Frame = 3
Query:  189 RKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACY-----QGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + D + R  +  A + GH EIV+ L++H  ++   D   +  + +A       +GH+  V LLI+   +++  DK    P+      G++++V+LL+E   D++  D     PL  A    +  +V  L
Sbjct:  672 KADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTL 800          
BLAST of SMED30016539 vs. Ensembl Human
Match: ANKRD50 (ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:HGNC:29223])

HSP 1 Score: 77.0258 bits (188), Expect = 3.862e-16
Identity = 42/134 (31.34%), Postives = 77/134 (57.46%), Query Frame = 3
Query:  165 YFKEEFS--ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQPIHWEC---ESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            YF E  +  E  D + R  +H +C+ GH E+V++LI ++ D+ A D +K+  +  A +QGH + V+LLIEH   ++    Q     C   + G+I++V++L+E   D N  D+     + +A KN + +++++L
Sbjct:  785 YFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLL 918          

HSP 2 Score: 58.5362 bits (140), Expect = 8.632e-10
Identity = 40/153 (26.14%), Postives = 77/153 (50.33%), Query Frame = 3
Query:  126 NAIRSGSVELFRRYFKEEFSER-----KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK------QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            NA+R  ++E  R   +  FS       KD D R  ++         + +  +E+  ++EA D + +  +H++C+QGH E V++LI ++ D+ A D       Q   W+   G++++V+LLIE    ++         L IA +  + ++V++L
Sbjct:  736 NALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQ---GHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVL 885          

HSP 3 Score: 57.7658 bits (138), Expect = 1.497e-9
Identity = 35/127 (27.56%), Postives = 63/127 (49.61%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--------ALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            D D R  +  A + GH +IV  L++H  ++   D + +  +  A Y GH E V+ L++H  ++         AL    +      G+  +V LLI+R  +++  DK  M PL +A    + ++V++L
Sbjct:  462 DADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLL 588          

HSP 4 Score: 56.225 bits (134), Expect = 5.757e-9
Identity = 41/135 (30.37%), Postives = 64/135 (47.41%), Query Frame = 3
Query:   96 SMDEEFKLFIN-AIRSGSVELFRRYFKEEFSE-RKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH--NCDMEALD-KQPIHWECESGYIEIVELLIERKCDINARD 485
            S+D E +  ++ A   G+VE+ R        E  +D     P+H A F GH  I + LIE       +D   + P  LA  +GH + V++L+E+  N D    D +  +      G+ +IVELL     D+N +D
Sbjct:  630 SIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKD 764          

HSP 5 Score: 52.7582 bits (125), Expect = 8.953e-8
Identity = 37/128 (28.91%), Postives = 60/128 (46.88%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFA-----GHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +DVD R  +  A        GHA +V LLI+   +++  DK    P+ +A Y+GH + V LL+E   D++  D   + P+      G+  +V  L+     +++ D      L IA    N E+V  L
Sbjct:  527 EDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTL 654          

HSP 6 Score: 48.1358 bits (113), Expect = 3.430e-6
Identity = 33/129 (25.58%), Postives = 62/129 (48.06%), Query Frame = 3
Query:  189 RKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACY-----QGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + D + R  +  A + GH EIV+ L++H  ++   D   +  + +A       +GH+  V LLI+   +++  DK    P+      G++++V+LL+E   D++  D     PL  A    +  +V  L
Sbjct:  493 KADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTL 621          
BLAST of SMED30016539 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 68.5514 bits (166), Expect = 1.113e-13
Identity = 36/120 (30.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +L+ P+H A   G+ +IV LL++   +  A  +    P+H+A  +G  E   +L++HN D   L K+   P+H   + G +E+V LL+ER   ++   K ++ PL +A    N ++  +L
Sbjct:  490 ELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLL 609          

HSP 2 Score: 65.0846 bits (157), Expect = 2.295e-12
Identity = 36/120 (30.00%), Postives = 60/120 (50.00%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            DL  P+H A  +GH ++V LL+     I A  K    P+H+A    H +  + L+ H   ++ +      P+H     G++ + +LL++R  D N+R      PL IA K    ++VE+L
Sbjct:  292 DLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELL 411          

HSP 3 Score: 58.151 bits (139), Expect = 4.182e-10
Identity = 32/116 (27.59%), Postives = 61/116 (52.59%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+ E+V+LL+E    ++   K +  P+H+A +  + +   LL+E+    +A  K    P+H   +   +EI   L++ K D NA+ +    PL ++ +  +KE+  +L
Sbjct:  560 PLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL 675          

HSP 4 Score: 55.8398 bits (133), Expect = 3.153e-9
Identity = 32/116 (27.59%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A  A   ++V++LI +   ++A  ++ Q P+H+A   G+++ V LL++   +  A  +    P+H   + G  E+  +L++   D     K    PL +A K  N E+V +L
Sbjct:  461 PLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL 576          

HSP 5 Score: 53.1434 bits (126), Expect = 2.321e-8
Identity = 33/116 (28.45%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F G   IV  L++   + +    + + P+HLA     ++ V++LI +   ++A  ++   P+H     G  +IV LL++   + NA  +   +PL IA K   +E+  IL
Sbjct:  428 PLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGIL 543          

HSP 6 Score: 52.373 bits (124), Expect = 4.848e-8
Identity = 32/121 (26.45%), Postives = 59/121 (48.76%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VD   P+H A   GH  + KLL++ + D  +       P+H+AC +   + V+LL+++   +EA  +    P+H     G I IV  L+++  + +        PL +A +    ++V +L
Sbjct:  357 VDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVL 477          

HSP 7 Score: 51.9878 bits (123), Expect = 7.004e-8
Identity = 29/116 (25.00%), Postives = 58/116 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GH  + +LL+E   ++    +    P+H+A   G +    LL+     +++  K    P+H    SG+ ++V+LL+ +   I+A+ K  + PL +A + ++ +    L
Sbjct:  230 PLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTL 345          

HSP 8 Score: 50.447 bits (119), Expect = 2.052e-7
Identity = 26/83 (31.33%), Postives = 42/83 (50.60%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNC---DMEALDKQPIHWECESGYIEIVELL 452
            P+H AC  G   +VK L+E+  D+    +    P+H A  QGH+  V+ L+E+     +  A  + P+      GY+ +VE L
Sbjct:  725 PLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 46.595 bits (109), Expect = 4.685e-6
Identity = 29/116 (25.00%), Postives = 51/116 (43.97%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A      EI   L++   D  A  +    P+HL+  +GH E   LLIE+  D+ A        +H   +  ++ + ++L     +IN++      PL +A       +V+ L
Sbjct:  626 PLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFL 741          

HSP 10 Score: 45.8246 bits (107), Expect = 7.623e-6
Identity = 34/138 (24.64%), Postives = 67/138 (48.55%), Query Frame = 3
Query:  150 ELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ----PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            EL +R  + + + RK       +H A  AG + IV +L+E+  ++         P+++A  + H E VK L++H  + +AL  +    P+    + G+  +V +L+E     +++ K  +  L IA K ++     +L
Sbjct:   84 ELIKRQAQVDAATRKG---NTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGAN-QALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLL 213          

HSP 11 Score: 45.4394 bits (106), Expect = 9.022e-6
Identity = 31/148 (20.95%), Postives = 73/148 (49.32%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH--NCDMEALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            F+ A R+G +E      +        + +    +H A   GH+E+V+ LI+    ++A  ++    +H+A   G S  V +L+E+  N ++++++   P++   +  + E+V+ L++   +     +    PL +A++  +  +V +L
Sbjct:   37 FLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVL 184          
BLAST of SMED30016539 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 68.5514 bits (166), Expect = 1.113e-13
Identity = 36/120 (30.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +L+ P+H A   G+ +IV LL++   +  A  +    P+H+A  +G  E   +L++HN D   L K+   P+H   + G +E+V LL+ER   ++   K ++ PL +A    N ++  +L
Sbjct:  490 ELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLL 609          

HSP 2 Score: 65.0846 bits (157), Expect = 2.295e-12
Identity = 36/120 (30.00%), Postives = 60/120 (50.00%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            DL  P+H A  +GH ++V LL+     I A  K    P+H+A    H +  + L+ H   ++ +      P+H     G++ + +LL++R  D N+R      PL IA K    ++VE+L
Sbjct:  292 DLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELL 411          

HSP 3 Score: 58.151 bits (139), Expect = 4.182e-10
Identity = 32/116 (27.59%), Postives = 61/116 (52.59%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+ E+V+LL+E    ++   K +  P+H+A +  + +   LL+E+    +A  K    P+H   +   +EI   L++ K D NA+ +    PL ++ +  +KE+  +L
Sbjct:  560 PLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL 675          

HSP 4 Score: 55.8398 bits (133), Expect = 3.153e-9
Identity = 32/116 (27.59%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A  A   ++V++LI +   ++A  ++ Q P+H+A   G+++ V LL++   +  A  +    P+H   + G  E+  +L++   D     K    PL +A K  N E+V +L
Sbjct:  461 PLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL 576          

HSP 5 Score: 53.1434 bits (126), Expect = 2.321e-8
Identity = 33/116 (28.45%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F G   IV  L++   + +    + + P+HLA     ++ V++LI +   ++A  ++   P+H     G  +IV LL++   + NA  +   +PL IA K   +E+  IL
Sbjct:  428 PLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGIL 543          

HSP 6 Score: 52.373 bits (124), Expect = 4.848e-8
Identity = 32/121 (26.45%), Postives = 59/121 (48.76%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VD   P+H A   GH  + KLL++ + D  +       P+H+AC +   + V+LL+++   +EA  +    P+H     G I IV  L+++  + +        PL +A +    ++V +L
Sbjct:  357 VDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVL 477          

HSP 7 Score: 51.9878 bits (123), Expect = 7.004e-8
Identity = 29/116 (25.00%), Postives = 58/116 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GH  + +LL+E   ++    +    P+H+A   G +    LL+     +++  K    P+H    SG+ ++V+LL+ +   I+A+ K  + PL +A + ++ +    L
Sbjct:  230 PLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTL 345          

HSP 8 Score: 50.447 bits (119), Expect = 2.052e-7
Identity = 26/83 (31.33%), Postives = 42/83 (50.60%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNC---DMEALDKQPIHWECESGYIEIVELL 452
            P+H AC  G   +VK L+E+  D+    +    P+H A  QGH+  V+ L+E+     +  A  + P+      GY+ +VE L
Sbjct:  725 PLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 46.595 bits (109), Expect = 4.685e-6
Identity = 29/116 (25.00%), Postives = 51/116 (43.97%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A      EI   L++   D  A  +    P+HL+  +GH E   LLIE+  D+ A        +H   +  ++ + ++L     +IN++      PL +A       +V+ L
Sbjct:  626 PLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFL 741          

HSP 10 Score: 45.8246 bits (107), Expect = 7.623e-6
Identity = 34/138 (24.64%), Postives = 67/138 (48.55%), Query Frame = 3
Query:  150 ELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ----PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            EL +R  + + + RK       +H A  AG + IV +L+E+  ++         P+++A  + H E VK L++H  + +AL  +    P+    + G+  +V +L+E     +++ K  +  L IA K ++     +L
Sbjct:   84 ELIKRQAQVDAATRKG---NTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGAN-QALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLL 213          

HSP 11 Score: 45.4394 bits (106), Expect = 9.022e-6
Identity = 31/148 (20.95%), Postives = 73/148 (49.32%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH--NCDMEALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            F+ A R+G +E      +        + +    +H A   GH+E+V+ LI+    ++A  ++    +H+A   G S  V +L+E+  N ++++++   P++   +  + E+V+ L++   +     +    PL +A++  +  +V +L
Sbjct:   37 FLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVL 184          
BLAST of SMED30016539 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 68.5514 bits (166), Expect = 1.359e-13
Identity = 36/120 (30.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +L+ P+H A   G+ +IV LL++   +  A  +    P+H+A  +G  E   +L++HN D   L K+   P+H   + G +E+V LL+ER   ++   K ++ PL +A    N ++  +L
Sbjct:  490 ELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLL 609          

HSP 2 Score: 64.6994 bits (156), Expect = 2.855e-12
Identity = 36/120 (30.00%), Postives = 60/120 (50.00%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            DL  P+H A  +GH ++V LL+     I A  K    P+H+A    H +  + L+ H   ++ +      P+H     G++ + +LL++R  D N+R      PL IA K    ++VE+L
Sbjct:  292 DLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELL 411          

HSP 3 Score: 58.151 bits (139), Expect = 5.004e-10
Identity = 32/116 (27.59%), Postives = 61/116 (52.59%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+ E+V+LL+E    ++   K +  P+H+A +  + +   LL+E+    +A  K    P+H   +   +EI   L++ K D NA+ +    PL ++ +  +KE+  +L
Sbjct:  560 PLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL 675          

HSP 4 Score: 55.4546 bits (132), Expect = 3.735e-9
Identity = 32/116 (27.59%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A  A   ++V++LI +   ++A  ++ Q P+H+A   G+++ V LL++   +  A  +    P+H   + G  E+  +L++   D     K    PL +A K  N E+V +L
Sbjct:  461 PLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL 576          

HSP 5 Score: 53.1434 bits (126), Expect = 2.622e-8
Identity = 33/116 (28.45%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F G   IV  L++   + +    + + P+HLA     ++ V++LI +   ++A  ++   P+H     G  +IV LL++   + NA  +   +PL IA K   +E+  IL
Sbjct:  428 PLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGIL 543          

HSP 6 Score: 51.9878 bits (123), Expect = 5.580e-8
Identity = 32/121 (26.45%), Postives = 59/121 (48.76%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VD   P+H A   GH  + KLL++ + D  +       P+H+AC +   + V+LL+++   +EA  +    P+H     G I IV  L+++  + +        PL +A +    ++V +L
Sbjct:  357 VDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVL 477          

HSP 7 Score: 51.6026 bits (122), Expect = 7.986e-8
Identity = 29/116 (25.00%), Postives = 58/116 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GH  + +LL+E   ++    +    P+H+A   G +    LL+     +++  K    P+H    SG+ ++V+LL+ +   I+A+ K  + PL +A + ++ +    L
Sbjct:  230 PLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTL 345          

HSP 8 Score: 50.0618 bits (118), Expect = 2.498e-7
Identity = 26/83 (31.33%), Postives = 42/83 (50.60%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNC---DMEALDKQPIHWECESGYIEIVELL 452
            P+H AC  G   +VK L+E+  D+    +    P+H A  QGH+  V+ L+E+     +  A  + P+      GY+ +VE L
Sbjct:  725 PLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 46.2098 bits (108), Expect = 5.540e-6
Identity = 29/116 (25.00%), Postives = 51/116 (43.97%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A      EI   L++   D  A  +    P+HL+  +GH E   LLIE+  D+ A        +H   +  ++ + ++L     +IN++      PL +A       +V+ L
Sbjct:  626 PLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFL 741          

HSP 10 Score: 45.4394 bits (106), Expect = 9.014e-6
Identity = 34/138 (24.64%), Postives = 67/138 (48.55%), Query Frame = 3
Query:  150 ELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ----PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            EL +R  + + + RK       +H A  AG + IV +L+E+  ++         P+++A  + H E VK L++H  + +AL  +    P+    + G+  +V +L+E     +++ K  +  L IA K ++     +L
Sbjct:   84 ELIKRQAQVDAATRKG---NTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGAN-QALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLL 213          
BLAST of SMED30016539 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 68.5514 bits (166), Expect = 1.359e-13
Identity = 36/120 (30.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +L+ P+H A   G+ +IV LL++   +  A  +    P+H+A  +G  E   +L++HN D   L K+   P+H   + G +E+V LL+ER   ++   K ++ PL +A    N ++  +L
Sbjct:  490 ELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLL 609          

HSP 2 Score: 64.6994 bits (156), Expect = 2.855e-12
Identity = 36/120 (30.00%), Postives = 60/120 (50.00%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            DL  P+H A  +GH ++V LL+     I A  K    P+H+A    H +  + L+ H   ++ +      P+H     G++ + +LL++R  D N+R      PL IA K    ++VE+L
Sbjct:  292 DLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELL 411          

HSP 3 Score: 58.151 bits (139), Expect = 5.004e-10
Identity = 32/116 (27.59%), Postives = 61/116 (52.59%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+ E+V+LL+E    ++   K +  P+H+A +  + +   LL+E+    +A  K    P+H   +   +EI   L++ K D NA+ +    PL ++ +  +KE+  +L
Sbjct:  560 PLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL 675          

HSP 4 Score: 55.4546 bits (132), Expect = 3.735e-9
Identity = 32/116 (27.59%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A  A   ++V++LI +   ++A  ++ Q P+H+A   G+++ V LL++   +  A  +    P+H   + G  E+  +L++   D     K    PL +A K  N E+V +L
Sbjct:  461 PLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL 576          

HSP 5 Score: 53.1434 bits (126), Expect = 2.622e-8
Identity = 33/116 (28.45%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F G   IV  L++   + +    + + P+HLA     ++ V++LI +   ++A  ++   P+H     G  +IV LL++   + NA  +   +PL IA K   +E+  IL
Sbjct:  428 PLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGIL 543          

HSP 6 Score: 51.9878 bits (123), Expect = 5.580e-8
Identity = 32/121 (26.45%), Postives = 59/121 (48.76%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VD   P+H A   GH  + KLL++ + D  +       P+H+AC +   + V+LL+++   +EA  +    P+H     G I IV  L+++  + +        PL +A +    ++V +L
Sbjct:  357 VDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVL 477          

HSP 7 Score: 51.6026 bits (122), Expect = 7.986e-8
Identity = 29/116 (25.00%), Postives = 58/116 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GH  + +LL+E   ++    +    P+H+A   G +    LL+     +++  K    P+H    SG+ ++V+LL+ +   I+A+ K  + PL +A + ++ +    L
Sbjct:  230 PLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTL 345          

HSP 8 Score: 50.0618 bits (118), Expect = 2.498e-7
Identity = 26/83 (31.33%), Postives = 42/83 (50.60%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNC---DMEALDKQPIHWECESGYIEIVELL 452
            P+H AC  G   +VK L+E+  D+    +    P+H A  QGH+  V+ L+E+     +  A  + P+      GY+ +VE L
Sbjct:  725 PLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 46.2098 bits (108), Expect = 5.540e-6
Identity = 29/116 (25.00%), Postives = 51/116 (43.97%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A      EI   L++   D  A  +    P+HL+  +GH E   LLIE+  D+ A        +H   +  ++ + ++L     +IN++      PL +A       +V+ L
Sbjct:  626 PLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFL 741          

HSP 10 Score: 45.4394 bits (106), Expect = 9.014e-6
Identity = 34/138 (24.64%), Postives = 67/138 (48.55%), Query Frame = 3
Query:  150 ELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ----PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            EL +R  + + + RK       +H A  AG + IV +L+E+  ++         P+++A  + H E VK L++H  + +AL  +    P+    + G+  +V +L+E     +++ K  +  L IA K ++     +L
Sbjct:   84 ELIKRQAQVDAATRKG---NTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGAN-QALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLL 213          
BLAST of SMED30016539 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 68.5514 bits (166), Expect = 1.362e-13
Identity = 36/120 (30.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +L+ P+H A   G+ +IV LL++   +  A  +    P+H+A  +G  E   +L++HN D   L K+   P+H   + G +E+V LL+ER   ++   K ++ PL +A    N ++  +L
Sbjct:  490 ELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLL 609          

HSP 2 Score: 64.6994 bits (156), Expect = 2.861e-12
Identity = 36/120 (30.00%), Postives = 60/120 (50.00%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            DL  P+H A  +GH ++V LL+     I A  K    P+H+A    H +  + L+ H   ++ +      P+H     G++ + +LL++R  D N+R      PL IA K    ++VE+L
Sbjct:  292 DLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELL 411          

HSP 3 Score: 58.151 bits (139), Expect = 5.061e-10
Identity = 32/116 (27.59%), Postives = 61/116 (52.59%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+ E+V+LL+E    ++   K +  P+H+A +  + +   LL+E+    +A  K    P+H   +   +EI   L++ K D NA+ +    PL ++ +  +KE+  +L
Sbjct:  560 PLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL 675          

HSP 4 Score: 55.4546 bits (132), Expect = 3.777e-9
Identity = 32/116 (27.59%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A  A   ++V++LI +   ++A  ++ Q P+H+A   G+++ V LL++   +  A  +    P+H   + G  E+  +L++   D     K    PL +A K  N E+V +L
Sbjct:  461 PLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL 576          

HSP 5 Score: 53.1434 bits (126), Expect = 2.651e-8
Identity = 33/116 (28.45%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F G   IV  L++   + +    + + P+HLA     ++ V++LI +   ++A  ++   P+H     G  +IV LL++   + NA  +   +PL IA K   +E+  IL
Sbjct:  428 PLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGIL 543          

HSP 6 Score: 51.9878 bits (123), Expect = 5.590e-8
Identity = 32/121 (26.45%), Postives = 59/121 (48.76%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VD   P+H A   GH  + KLL++ + D  +       P+H+AC +   + V+LL+++   +EA  +    P+H     G I IV  L+++  + +        PL +A +    ++V +L
Sbjct:  357 VDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVL 477          

HSP 7 Score: 51.6026 bits (122), Expect = 8.000e-8
Identity = 29/116 (25.00%), Postives = 58/116 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GH  + +LL+E   ++    +    P+H+A   G +    LL+     +++  K    P+H    SG+ ++V+LL+ +   I+A+ K  + PL +A + ++ +    L
Sbjct:  230 PLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTL 345          

HSP 8 Score: 50.0618 bits (118), Expect = 2.526e-7
Identity = 26/83 (31.33%), Postives = 42/83 (50.60%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNC---DMEALDKQPIHWECESGYIEIVELL 452
            P+H AC  G   +VK L+E+  D+    +    P+H A  QGH+  V+ L+E+     +  A  + P+      GY+ +VE L
Sbjct:  725 PLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 46.2098 bits (108), Expect = 5.602e-6
Identity = 29/116 (25.00%), Postives = 51/116 (43.97%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A      EI   L++   D  A  +    P+HL+  +GH E   LLIE+  D+ A        +H   +  ++ + ++L     +IN++      PL +A       +V+ L
Sbjct:  626 PLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFL 741          

HSP 10 Score: 45.4394 bits (106), Expect = 9.029e-6
Identity = 34/138 (24.64%), Postives = 67/138 (48.55%), Query Frame = 3
Query:  150 ELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ----PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            EL +R  + + + RK       +H A  AG + IV +L+E+  ++         P+++A  + H E VK L++H  + +AL  +    P+    + G+  +V +L+E     +++ K  +  L IA K ++     +L
Sbjct:   84 ELIKRQAQVDAATRKG---NTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGAN-QALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLL 213          
BLAST of SMED30016539 vs. Ensembl Fly
Match: CG11168 (gene:FBgn0039249 transcript:FBtr0306089)

HSP 1 Score: 72.0182 bits (175), Expect = 4.862e-15
Identity = 41/145 (28.28%), Postives = 74/145 (51.03%), Query Frame = 3
Query:  135 RSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-----NCDMEALDKQ-PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            R G +  FRR         +D++    +HHAC  GH+ IV+LL+ HN  ++  D +   P+ LA + GH + VK+L+ H     N + + ++ + P+H   + G+  +V +L+    D   R+      LD+A +    ++V+ L
Sbjct:   29 RHGPLSSFRR---SPSINCQDMNGYTSLHHACLNGHSNIVRLLLSHNALLDVPDIRGSTPLFLAAWAGHQDIVKMLLMHSPTGANPNAQTIENETPLHSGAQHGHNAVVAILLSYGADPAIRNNSFQTALDLAAQFGRLQVVQTL 170          
BLAST of SMED30016539 vs. Ensembl Fly
Match: CG4393 (gene:FBgn0039075 transcript:FBtr0339617)

HSP 1 Score: 70.8626 bits (172), Expect = 2.368e-14
Identity = 36/118 (30.51%), Postives = 65/118 (55.08%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-----NCDMEALDKQ-PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +HHAC  GH +IV+LL+ H       D +   P+HLA + G +E V+LL+ H     + +++ ++++ P+H   + G+   + LL+    D N R+     PLD+A +    + V++L
Sbjct:   53 LHHACLNGHEDIVRLLLAHEASPNLPDSRGSSPLHLAAWAGETEIVRLLLTHPYRPASANLQTIEQETPLHCAAQHGHTGALALLLHHDADPNMRNSRGETPLDLAAQYGRLQAVQML 170          

HSP 2 Score: 46.9802 bits (110), Expect = 4.262e-6
Identity = 26/87 (29.89%), Postives = 47/87 (54.02%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQ---KQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLI 455
            P+H A +AG  EIV+LL+ H     + + Q   ++ P+H A   GH+  + LL+ H+ D    + +   P+    + G ++ V++LI
Sbjct:   85 PLHLAAWAGETEIVRLLLTHPYRPASANLQTIEQETPLHCAAQHGHTGALALLLHHDADPNMRNSRGETPLDLAAQYGRLQAVQMLI 171          
BLAST of SMED30016539 vs. Ensembl Fly
Match: CG4393 (gene:FBgn0039075 transcript:FBtr0301085)

HSP 1 Score: 70.8626 bits (172), Expect = 2.368e-14
Identity = 36/118 (30.51%), Postives = 65/118 (55.08%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-----NCDMEALDKQ-PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +HHAC  GH +IV+LL+ H       D +   P+HLA + G +E V+LL+ H     + +++ ++++ P+H   + G+   + LL+    D N R+     PLD+A +    + V++L
Sbjct:   53 LHHACLNGHEDIVRLLLAHEASPNLPDSRGSSPLHLAAWAGETEIVRLLLTHPYRPASANLQTIEQETPLHCAAQHGHTGALALLLHHDADPNMRNSRGETPLDLAAQYGRLQAVQML 170          

HSP 2 Score: 46.9802 bits (110), Expect = 4.262e-6
Identity = 26/87 (29.89%), Postives = 47/87 (54.02%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQ---KQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLI 455
            P+H A +AG  EIV+LL+ H     + + Q   ++ P+H A   GH+  + LL+ H+ D    + +   P+    + G ++ V++LI
Sbjct:   85 PLHLAAWAGETEIVRLLLTHPYRPASANLQTIEQETPLHCAAQHGHTGALALLLHHDADPNMRNSRGETPLDLAAQYGRLQAVQMLI 171          
BLAST of SMED30016539 vs. Ensembl Fly
Match: CG4393 (gene:FBgn0039075 transcript:FBtr0339616)

HSP 1 Score: 70.8626 bits (172), Expect = 2.368e-14
Identity = 36/118 (30.51%), Postives = 65/118 (55.08%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-----NCDMEALDKQ-PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +HHAC  GH +IV+LL+ H       D +   P+HLA + G +E V+LL+ H     + +++ ++++ P+H   + G+   + LL+    D N R+     PLD+A +    + V++L
Sbjct:   53 LHHACLNGHEDIVRLLLAHEASPNLPDSRGSSPLHLAAWAGETEIVRLLLTHPYRPASANLQTIEQETPLHCAAQHGHTGALALLLHHDADPNMRNSRGETPLDLAAQYGRLQAVQML 170          

HSP 2 Score: 46.9802 bits (110), Expect = 4.262e-6
Identity = 26/87 (29.89%), Postives = 47/87 (54.02%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQ---KQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLI 455
            P+H A +AG  EIV+LL+ H     + + Q   ++ P+H A   GH+  + LL+ H+ D    + +   P+    + G ++ V++LI
Sbjct:   85 PLHLAAWAGETEIVRLLLTHPYRPASANLQTIEQETPLHCAAQHGHTGALALLLHHDADPNMRNSRGETPLDLAAQYGRLQAVQMLI 171          
BLAST of SMED30016539 vs. Ensembl Fly
Match: CG42672 (gene:FBgn0261554 transcript:FBtr0302702)

HSP 1 Score: 68.9366 bits (167), Expect = 1.251e-13
Identity = 40/116 (34.48%), Postives = 66/116 (56.90%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+ HA  AGH  +V+ L++ + D++   K ++  I+ A  +GH+  VKLL+  N D+E+  K    P+     +  +EIV LL++RK  + A DK     L IA++  +K +VE L
Sbjct:  273 PLIHAVKAGHRTVVEALLKKHADVDIQGKDRKTAIYTAVEKGHTPIVKLLLATNPDLESATKDGDTPLLRAVRNRNLEIVHLLLDRKAKVTASDKRGDTCLHIAMRARSKTIVEAL 388          

HSP 2 Score: 64.3142 bits (155), Expect = 4.571e-12
Identity = 43/137 (31.39%), Postives = 67/137 (48.91%), Query Frame = 3
Query:  168 FKEEFSER------KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            F  EF  R      +D+D    +  A   GH ++V+LL++H  ++E  D      +  A Y+GH+E V+LL++   D  A        + W    GY +IVELL++R   +N  DK     L  A +  N E+V+ L
Sbjct:   54 FVREFLARGADVQAEDLDNWTALLCASRNGHLDVVQLLLDHGAEVEHRDMGGWTSLMWAAYRGHTELVRLLLDKGADGNAHGNYHLGALLWAAGRGYKDIVELLVQRGAKVNVGDKYGTTALVWACRRGNVEIVDTL 190          

HSP 3 Score: 47.7506 bits (112), Expect = 2.188e-6
Identity = 32/112 (28.57%), Postives = 55/112 (49.11%), Query Frame = 3
Query:  225 ACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQPIHWEC---ESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            AC  G+ EIV  L++   +++        P+ +A   GH++ V  ++E   ++ ALDK  +   C     G+ +I   LI     IN +D+    PL  A+K  ++ +VE L
Sbjct:  178 ACRRGNVEIVDTLLKAGANVDTAGMYSWTPLLVAAAGGHTDCVSSILEKKPNVNALDKDGMTALCIASREGFQDIAASLIAAGAYINIQDRGADTPLIHAVKAGHRTVVEAL 289          
BLAST of SMED30016539 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 88.9669 bits (219), Expect = 1.745e-20
Identity = 45/145 (31.03%), Postives = 83/145 (57.24%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R+G  E+ +   +     + K  D + P+H A   GH E+VKLL+EH  + ++       P+H+A  +GH++  ++L++ N     + K+   P+H  C+ G +++VELL+ER  + NA  K  + PL +A+ + N ++V++L
Sbjct:  477 AARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 621          

HSP 2 Score: 69.3218 bits (168), Expect = 1.280e-13
Identity = 36/116 (31.03%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEAL---DKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F GH  IVK L++      A + + + P+H+A   GH E  + L+++N  ++A    D+ P+H     G+ E+V+LL+E K + ++       PL IA +  + +   IL
Sbjct:  440 PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRIL 555          

HSP 3 Score: 60.8474 bits (146), Expect = 1.054e-10
Identity = 40/145 (27.59%), Postives = 72/145 (49.66%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--ALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R G+V + R         + K  D   P+H A   GH  +V++L++    ++A  K    PIH+A    H + V+ L+++N +++   LD   P+H     G+  +V++L+++    NAR      PL IA K  +   +++L
Sbjct:  279 ASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLL 423          

HSP 4 Score: 60.4622 bits (145), Expect = 1.275e-10
Identity = 34/121 (28.10%), Postives = 62/121 (51.24%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   P+H A   GH  +VK+L++      A       P+H+AC + H   + LL++H+  +EA+ +    P+H     G++ IV+ L++R    NA +     PL +A +  + E+ + L
Sbjct:  369 LDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFL 489          

HSP 5 Score: 53.5286 bits (127), Expect = 2.921e-8
Identity = 30/116 (25.86%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+  +V+LL++    I+A  K +  P+H A   GH   V++L++    ++A  K    PIH   +  +++ V  L++   +I+      + PL +A    +  +V++L
Sbjct:  275 PLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVL 390          

HSP 6 Score: 53.1434 bits (126), Expect = 4.512e-8
Identity = 27/116 (23.28%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A    +  + +LL+    ++    K    P+H+A  +G+   V+LL++    ++A  K    P+H    +G++ +VE+L+++   + A+ K  ++P+ +A + ++ + V  L
Sbjct:  242 PLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQL 357          

HSP 7 Score: 52.7582 bits (125), Expect = 6.047e-8
Identity = 26/102 (25.49%), Postives = 52/102 (50.98%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLD 509
            P+H A   G  ++V LLI    ++   +K    P+HL   +GH     +L++    + A  +    P+H  C  G I++V+ L++++  +N++ +  +  LD
Sbjct:  671 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRVSVLLLD 772          

HSP 8 Score: 49.2914 bits (116), Expect = 9.572e-7
Identity = 34/152 (22.37%), Postives = 74/152 (48.68%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRYFKEEFSERKDVDLRRP-----IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            F+ A RSG++E    + K       D+++        +H A   GH ++V  L+ +  D+E   K+    +H+A   G  + V  LI +  ++ A  ++   P++   +  ++E+V+ L+E   + +   +    PL +A++  ++ +V +L
Sbjct:   49 FLRAARSGNLEKALDHIKNGI----DINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALL 196          

HSP 9 Score: 47.3654 bits (111), Expect = 3.809e-6
Identity = 27/115 (23.48%), Postives = 55/115 (47.83%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A      E+   L+++  +  +   Q   P+HLA  +G  + V LLI    ++   +K    P+H   + G++ I ++L+++   + A  +    PL +A    N ++V+ L
Sbjct:  639 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFL 753          

HSP 10 Score: 46.595 bits (109), Expect = 5.918e-6
Identity = 27/116 (23.28%), Postives = 55/116 (47.41%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC  G  ++V+LL+E   +  A  K    P+H+A +  + + VKLL+       +  +     +H   +   +E+   L++   + N+     + PL +A +    ++V +L
Sbjct:  572 PLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALL 687          
BLAST of SMED30016539 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 88.9669 bits (219), Expect = 1.936e-20
Identity = 45/145 (31.03%), Postives = 83/145 (57.24%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R+G  E+ +   +     + K  D + P+H A   GH E+VKLL+EH  + ++       P+H+A  +GH++  ++L++ N     + K+   P+H  C+ G +++VELL+ER  + NA  K  + PL +A+ + N ++V++L
Sbjct:  385 AARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 529          

HSP 2 Score: 68.9366 bits (167), Expect = 1.354e-13
Identity = 36/116 (31.03%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEAL---DKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F GH  IVK L++      A + + + P+H+A   GH E  + L+++N  ++A    D+ P+H     G+ E+V+LL+E K + ++       PL IA +  + +   IL
Sbjct:  348 PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRIL 463          

HSP 3 Score: 60.4622 bits (145), Expect = 1.158e-10
Identity = 40/145 (27.59%), Postives = 72/145 (49.66%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--ALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R G+V + R         + K  D   P+H A   GH  +V++L++    ++A  K    PIH+A    H + V+ L+++N +++   LD   P+H     G+  +V++L+++    NAR      PL IA K  +   +++L
Sbjct:  187 ASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLL 331          

HSP 4 Score: 60.4622 bits (145), Expect = 1.361e-10
Identity = 34/121 (28.10%), Postives = 62/121 (51.24%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   P+H A   GH  +VK+L++      A       P+H+AC + H   + LL++H+  +EA+ +    P+H     G++ IV+ L++R    NA +     PL +A +  + E+ + L
Sbjct:  277 LDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFL 397          

HSP 5 Score: 57.7658 bits (138), Expect = 9.731e-10
Identity = 29/116 (25.00%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G  ++V LLI    ++   +K    P+HL   +GH     +L++    + A  +    P+H  C  G I++V+ L++++  +N++ +    PL  A +  + ++V +L
Sbjct:  579 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLL 694          

HSP 6 Score: 53.5286 bits (127), Expect = 3.119e-8
Identity = 30/116 (25.86%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+  +V+LL++    I+A  K +  P+H A   GH   V++L++    ++A  K    PIH   +  +++ V  L++   +I+      + PL +A    +  +V++L
Sbjct:  183 PLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVL 298          

HSP 7 Score: 52.7582 bits (125), Expect = 5.099e-8
Identity = 27/116 (23.28%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A    +  + +LL+    ++    K    P+H+A  +G+   V+LL++    ++A  K    P+H    +G++ +VE+L+++   + A+ K  ++P+ +A + ++ + V  L
Sbjct:  150 PLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQL 265          

HSP 8 Score: 51.2174 bits (121), Expect = 1.583e-7
Identity = 29/116 (25.00%), Postives = 56/116 (48.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H     GH  I  +L++    + A  +    P+H+AC+ G+ + VK L++    + +   L   P+H   + G+ +IV LL++     N       +PL IA +     ++++L
Sbjct:  612 PLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 727          

HSP 9 Score: 47.3654 bits (111), Expect = 4.261e-6
Identity = 27/115 (23.48%), Postives = 55/115 (47.83%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A      E+   L+++  +  +   Q   P+HLA  +G  + V LLI    ++   +K    P+H   + G++ I ++L+++   + A  +    PL +A    N ++V+ L
Sbjct:  547 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFL 661          

HSP 10 Score: 46.595 bits (109), Expect = 6.438e-6
Identity = 27/116 (23.28%), Postives = 55/116 (47.41%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC  G  ++V+LL+E   +  A  K    P+H+A +  + + VKLL+       +  +     +H   +   +E+   L++   + N+     + PL +A +    ++V +L
Sbjct:  480 PLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALL 595          
BLAST of SMED30016539 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 88.1965 bits (217), Expect = 3.124e-20
Identity = 45/145 (31.03%), Postives = 83/145 (57.24%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R+G  E+ +   +     + K  D + P+H A   GH E+VKLL+EH  + ++       P+H+A  +GH++  ++L++ N     + K+   P+H  C+ G +++VELL+ER  + NA  K  + PL +A+ + N ++V++L
Sbjct:  458 AARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 602          

HSP 2 Score: 63.1586 bits (152), Expect = 1.342e-11
Identity = 38/120 (31.67%), Postives = 64/120 (53.33%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEH----NCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEAL---DKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F GH  IVK L++     N +I + D   + P+H+A   GH E  + L+++N  ++A    D+ P+H     G+ E+V+LL+E K + ++       PL IA +  + +   IL
Sbjct:  418 PLHVAAFMGHLNIVKSLLQRGASPNSNIFSSD-YVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRIL 536          

HSP 3 Score: 60.077 bits (144), Expect = 1.624e-10
Identity = 40/145 (27.59%), Postives = 72/145 (49.66%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--ALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R G+V + R         + K  D   P+H A   GH  +V++L++    ++A  K    PIH+A    H + V+ L+++N +++   LD   P+H     G+  +V++L+++    NAR      PL IA K  +   +++L
Sbjct:  257 ASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLL 401          

HSP 4 Score: 57.7658 bits (138), Expect = 1.025e-9
Identity = 35/157 (22.29%), Postives = 66/157 (42.04%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---------------------------------------PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   P+H A   GH  +VK+L++      A       P+H+AC + H   + LL++H+  +EA+ +                                        P+H    +G+ E+ + L++    ++A+ K +  PL  A +  +KELV++L
Sbjct:  347 LDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLL 503          

HSP 5 Score: 57.3806 bits (137), Expect = 1.402e-9
Identity = 29/116 (25.00%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G  ++V LLI    ++   +K    P+HL   +GH     +L++    + A  +    P+H  C  G I++V+ L++++  +N++ +    PL  A +  + ++V +L
Sbjct:  652 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLL 767          

HSP 6 Score: 53.1434 bits (126), Expect = 4.043e-8
Identity = 30/116 (25.86%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+  +V+LL++    I+A  K +  P+H A   GH   V++L++    ++A  K    PIH   +  +++ V  L++   +I+      + PL +A    +  +V++L
Sbjct:  253 PLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVL 368          

HSP 7 Score: 52.373 bits (124), Expect = 6.861e-8
Identity = 27/116 (23.28%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A    +  + +LL+    ++    K    P+H+A  +G+   V+LL++    ++A  K    P+H    +G++ +VE+L+++   + A+ K  ++P+ +A + ++ + V  L
Sbjct:  220 PLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQL 335          

HSP 8 Score: 51.2174 bits (121), Expect = 2.149e-7
Identity = 29/116 (25.00%), Postives = 56/116 (48.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H     GH  I  +L++    + A  +    P+H+AC+ G+ + VK L++    + +   L   P+H   + G+ +IV LL++     N       +PL IA +     ++++L
Sbjct:  685 PLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 800          

HSP 9 Score: 46.9802 bits (110), Expect = 5.454e-6
Identity = 27/115 (23.48%), Postives = 55/115 (47.83%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A      E+   L+++  +  +   Q   P+HLA  +G  + V LLI    ++   +K    P+H   + G++ I ++L+++   + A  +    PL +A    N ++V+ L
Sbjct:  620 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFL 734          

HSP 10 Score: 46.2098 bits (108), Expect = 8.084e-6
Identity = 26/115 (22.61%), Postives = 59/115 (51.30%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A   GH ++V  L+ +  D+E   K+    +H+A   G  + V  LI +  ++ A  ++   P++   +  ++E+V+ L+E   + +   +    PL +A++  ++ +V +L
Sbjct:   60 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALL 174          

HSP 11 Score: 46.2098 bits (108), Expect = 8.393e-6
Identity = 27/116 (23.28%), Postives = 55/116 (47.41%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC  G  ++V+LL+E   +  A  K    P+H+A +  + + VKLL+       +  +     +H   +   +E+   L++   + N+     + PL +A +    ++V +L
Sbjct:  553 PLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALL 668          
BLAST of SMED30016539 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 87.8113 bits (216), Expect = 4.014e-20
Identity = 45/145 (31.03%), Postives = 83/145 (57.24%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R+G  E+ +   +     + K  D + P+H A   GH E+VKLL+EH  + ++       P+H+A  +GH++  ++L++ N     + K+   P+H  C+ G +++VELL+ER  + NA  K  + PL +A+ + N ++V++L
Sbjct:  477 AARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 621          

HSP 2 Score: 63.1586 bits (152), Expect = 1.609e-11
Identity = 38/120 (31.67%), Postives = 64/120 (53.33%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEH----NCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEAL---DKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F GH  IVK L++     N +I + D   + P+H+A   GH E  + L+++N  ++A    D+ P+H     G+ E+V+LL+E K + ++       PL IA +  + +   IL
Sbjct:  437 PLHVAAFMGHLNIVKSLLQRGASPNSNIFSSD-YVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRIL 555          

HSP 3 Score: 60.077 bits (144), Expect = 2.022e-10
Identity = 40/145 (27.59%), Postives = 72/145 (49.66%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--ALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R G+V + R         + K  D   P+H A   GH  +V++L++    ++A  K    PIH+A    H + V+ L+++N +++   LD   P+H     G+  +V++L+++    NAR      PL IA K  +   +++L
Sbjct:  276 ASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLL 420          

HSP 4 Score: 57.7658 bits (138), Expect = 1.252e-9
Identity = 35/157 (22.29%), Postives = 66/157 (42.04%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---------------------------------------PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   P+H A   GH  +VK+L++      A       P+H+AC + H   + LL++H+  +EA+ +                                        P+H    +G+ E+ + L++    ++A+ K +  PL  A +  +KELV++L
Sbjct:  366 LDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLL 522          

HSP 5 Score: 57.3806 bits (137), Expect = 1.634e-9
Identity = 29/116 (25.00%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G  ++V LLI    ++   +K    P+HL   +GH     +L++    + A  +    P+H  C  G I++V+ L++++  +N++ +    PL  A +  + ++V +L
Sbjct:  671 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLL 786          

HSP 6 Score: 53.1434 bits (126), Expect = 4.796e-8
Identity = 30/116 (25.86%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+  +V+LL++    I+A  K +  P+H A   GH   V++L++    ++A  K    PIH   +  +++ V  L++   +I+      + PL +A    +  +V++L
Sbjct:  272 PLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVL 387          

HSP 7 Score: 52.373 bits (124), Expect = 7.911e-8
Identity = 27/116 (23.28%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A    +  + +LL+    ++    K    P+H+A  +G+   V+LL++    ++A  K    P+H    +G++ +VE+L+++   + A+ K  ++P+ +A + ++ + V  L
Sbjct:  239 PLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQL 354          

HSP 8 Score: 50.8322 bits (120), Expect = 2.572e-7
Identity = 29/116 (25.00%), Postives = 56/116 (48.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H     GH  I  +L++    + A  +    P+H+AC+ G+ + VK L++    + +   L   P+H   + G+ +IV LL++     N       +PL IA +     ++++L
Sbjct:  704 PLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 819          

HSP 9 Score: 46.595 bits (109), Expect = 6.282e-6
Identity = 27/115 (23.48%), Postives = 55/115 (47.83%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A      E+   L+++  +  +   Q   P+HLA  +G  + V LLI    ++   +K    P+H   + G++ I ++L+++   + A  +    PL +A    N ++V+ L
Sbjct:  639 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFL 753          

HSP 10 Score: 46.2098 bits (108), Expect = 9.137e-6
Identity = 26/115 (22.61%), Postives = 59/115 (51.30%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A   GH ++V  L+ +  D+E   K+    +H+A   G  + V  LI +  ++ A  ++   P++   +  ++E+V+ L+E   + +   +    PL +A++  ++ +V +L
Sbjct:   79 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALL 193          

HSP 11 Score: 46.2098 bits (108), Expect = 9.665e-6
Identity = 27/116 (23.28%), Postives = 55/116 (47.41%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC  G  ++V+LL+E   +  A  K    P+H+A +  + + VKLL+       +  +     +H   +   +E+   L++   + N+     + PL +A +    ++V +L
Sbjct:  572 PLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALL 687          
BLAST of SMED30016539 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 87.8113 bits (216), Expect = 4.165e-20
Identity = 45/145 (31.03%), Postives = 83/145 (57.24%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R+G  E+ +   +     + K  D + P+H A   GH E+VKLL+EH  + ++       P+H+A  +GH++  ++L++ N     + K+   P+H  C+ G +++VELL+ER  + NA  K  + PL +A+ + N ++V++L
Sbjct:  447 AARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 591          

HSP 2 Score: 68.1662 bits (165), Expect = 2.509e-13
Identity = 36/116 (31.03%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEAL---DKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A F GH  IVK L++      A + + + P+H+A   GH E  + L+++N  ++A    D+ P+H     G+ E+V+LL+E K + ++       PL IA +  + +   IL
Sbjct:  410 PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRIL 525          

HSP 3 Score: 59.6918 bits (143), Expect = 2.159e-10
Identity = 40/145 (27.59%), Postives = 72/145 (49.66%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--ALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R G+V + R         + K  D   P+H A   GH  +V++L++    ++A  K    PIH+A    H + V+ L+++N +++   LD   P+H     G+  +V++L+++    NAR      PL IA K  +   +++L
Sbjct:  249 ASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLL 393          

HSP 4 Score: 59.6918 bits (143), Expect = 2.374e-10
Identity = 34/121 (28.10%), Postives = 62/121 (51.24%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   P+H A   GH  +VK+L++      A       P+H+AC + H   + LL++H+  +EA+ +    P+H     G++ IV+ L++R    NA +     PL +A +  + E+ + L
Sbjct:  339 LDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFL 459          

HSP 5 Score: 57.3806 bits (137), Expect = 1.678e-9
Identity = 29/116 (25.00%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G  ++V LLI    ++   +K    P+HL   +GH     +L++    + A  +    P+H  C  G I++V+ L++++  +N++ +    PL  A +  + ++V +L
Sbjct:  641 PLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLL 756          

HSP 6 Score: 52.7582 bits (125), Expect = 5.021e-8
Identity = 30/116 (25.86%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+  +V+LL++    I+A  K +  P+H A   GH   V++L++    ++A  K    PIH   +  +++ V  L++   +I+      + PL +A    +  +V++L
Sbjct:  245 PLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVL 360          

HSP 7 Score: 52.373 bits (124), Expect = 7.975e-8
Identity = 27/116 (23.28%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A    +  + +LL+    ++    K    P+H+A  +G+   V+LL++    ++A  K    P+H    +G++ +VE+L+++   + A+ K  ++P+ +A + ++ + V  L
Sbjct:  212 PLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQL 327          

HSP 8 Score: 50.8322 bits (120), Expect = 2.667e-7
Identity = 29/116 (25.00%), Postives = 56/116 (48.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H     GH  I  +L++    + A  +    P+H+AC+ G+ + VK L++    + +   L   P+H   + G+ +IV LL++     N       +PL IA +     ++++L
Sbjct:  674 PLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 789          

HSP 9 Score: 48.521 bits (114), Expect = 1.751e-6
Identity = 34/152 (22.37%), Postives = 74/152 (48.68%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRYFKEEFSERKDVDLRRP-----IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            F+ A RSG++E    + K       D+++        +H A   GH ++V  L+ +  D+E   K+    +H+A   G  + V  LI +  ++ A  ++   P++   +  ++E+V+ L+E   + +   +    PL +A++  ++ +V +L
Sbjct:   19 FLRAARSGNLEKALDHIKNGI----DINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALL 166          

HSP 10 Score: 46.595 bits (109), Expect = 6.575e-6
Identity = 27/115 (23.48%), Postives = 55/115 (47.83%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A      E+   L+++  +  +   Q   P+HLA  +G  + V LLI    ++   +K    P+H   + G++ I ++L+++   + A  +    PL +A    N ++V+ L
Sbjct:  609 LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFL 723          

HSP 11 Score: 46.2098 bits (108), Expect = 9.836e-6
Identity = 27/116 (23.28%), Postives = 55/116 (47.41%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC  G  ++V+LL+E   +  A  K    P+H+A +  + + VKLL+       +  +     +H   +   +E+   L++   + N+     + PL +A +    ++V +L
Sbjct:  542 PLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALL 657          
BLAST of SMED30016539 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 85.1149 bits (209), Expect = 5.733e-19
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V LL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +++ L++   D+N  +     PL +A  N
Sbjct:  143 RTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYN 251          

HSP 2 Score: 63.5438 bits (153), Expect = 1.536e-11
Identity = 39/119 (32.77%), Postives = 62/119 (52.10%), Query Frame = 3
Query:  204 LRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            ++ P+  A F G  + V+ LI    D+   D +K+ P+H A Y G +E ++LLI     + A D +   P+H    S   + V++L++   D+NARDK    PL IA  N+  +  E L
Sbjct:   10 IQPPLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEAL 128          

HSP 3 Score: 63.1586 bits (152), Expect = 2.427e-11
Identity = 42/148 (28.38%), Postives = 74/148 (50.00%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRY-FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
             + AI +G  +  R   FK+E    +D + R P+H A + G AEI++LLI     + A D +   P+H A      + V++L++H+ D+ A DK    P+H    +  ++  E L+    ++N  D+     L  A  + + E+V +L
Sbjct:   14 LVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLL 161          

HSP 4 Score: 62.3882 bits (150), Expect = 3.945e-11
Identity = 39/144 (27.08%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   + V++L++H+ D+ A DK  Q P+H+A                                  + GH E V LL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   72 KDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAA 215          

HSP 5 Score: 60.077 bits (144), Expect = 2.273e-10
Identity = 37/129 (28.68%), Postives = 63/129 (48.84%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK-------QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V+ L++HN +    D + + PIHLA   GH   +  L++    ++ +          P+HW C +G+   VELL+E++      +    +PL  A+ N+N+   E+L
Sbjct:  687 DAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEV-FQKMEGNSFSPLHCAVINDNEGAAEML 814          

HSP 6 Score: 53.5286 bits (127), Expect = 3.668e-8
Identity = 36/110 (32.73%), Postives = 56/110 (50.91%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            R PIH A   GH+E ++LLI  N D++A     D   Q P+ L+   GH+E V  L+    +++A DK     +H    +G+ E VE L++   +   RD     P+ +A
Sbjct:  625 RTPIHSAAINGHSECLRLLI-GNADVQAAVDIHDGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLA 733          

HSP 7 Score: 51.9878 bits (123), Expect = 1.351e-7
Identity = 37/161 (22.98%), Postives = 62/161 (38.51%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNC-------------------------------------DMEALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   R PIH A   GH  ++  L++    ++ +    D     P+H ACY GH   V+LL+E                                         +++  + P+H    + +IE ++LL+     +NA D     PL +A +N     VE+L
Sbjct:  722 RDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEVFQKMEGNSFSPLHCAVINDNEGAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVL 882          

HSP 8 Score: 51.6026 bits (122), Expect = 1.971e-7
Identity = 31/117 (26.50%), Postives = 60/117 (51.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   + +  ++  D     PL +++ N + E V
Sbjct:  560 PLHLAAYHGHHQALEVLVQSLLDLDVRNSTGRTPLDLAAFKGHVECVDVLINQGASILVKDYVVKRTPIHSAAINGHSECLRLLIGNADVQAAVDIHDGNGQTPLMLSVLNGHTECV 676          

HSP 9 Score: 50.0618 bits (118), Expect = 6.649e-7
Identity = 37/144 (25.69%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+   ++  ++++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  172 DKKD---RRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELIDCGANVNQVNERGFTPLHFAAASTHGALCLELLVCNGADVNIKSKDGKTPL 312          

HSP 10 Score: 49.2914 bits (116), Expect = 1.334e-6
Identity = 33/126 (26.19%), Postives = 57/126 (45.24%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINA----RDKCEMNPLDIAIKNENKELVEIL 551
            D + + P+  +   GH E V  L+    +++A DK  +  +H     GH E V+ L++HN +    D   + PIH     G+I ++  L++    ++      D     PL  A  N +   VE+L
Sbjct:  657 DGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELL 782          

HSP 11 Score: 46.9802 bits (110), Expect = 6.057e-6
Identity = 32/119 (26.89%), Postives = 55/119 (46.22%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARD 485
            G+ E+             D   R P+H A F  H E ++LL+ HN  + A+D   + P+ +A   G +  V++L+     D+   DK     +H  C S Y+ ++ +L  R   + AR+
Sbjct:  809 GAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVLVSSAKADLTLQDKNKNTALHLAC-SKYL-LLGILSCRPLHVAARN 925          
BLAST of SMED30016539 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 85.1149 bits (209), Expect = 5.754e-19
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V LL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +++ L++   D+N  +     PL +A  N
Sbjct:  160 RTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYN 268          

HSP 2 Score: 64.6994 bits (156), Expect = 6.113e-12
Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + L+ P+  A F G  + V+ LI    D+   D +K+ P+H A Y G +E ++LLI     + A D +   P+H    S   + V++L++   D+NARDK    PL IA  N+  +  E L
Sbjct:   25 LSLQPPLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEAL 145          

HSP 3 Score: 63.1586 bits (152), Expect = 2.412e-11
Identity = 42/148 (28.38%), Postives = 74/148 (50.00%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRY-FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
             + AI +G  +  R   FK+E    +D + R P+H A + G AEI++LLI     + A D +   P+H A      + V++L++H+ D+ A DK    P+H    +  ++  E L+    ++N  D+     L  A  + + E+V +L
Sbjct:   31 LVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLL 178          

HSP 4 Score: 62.3882 bits (150), Expect = 3.996e-11
Identity = 39/144 (27.08%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   + V++L++H+ D+ A DK  Q P+H+A                                  + GH E V LL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   89 KDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAA 232          

HSP 5 Score: 60.077 bits (144), Expect = 2.280e-10
Identity = 37/129 (28.68%), Postives = 63/129 (48.84%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK-------QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V+ L++HN +    D + + PIHLA   GH   +  L++    ++ +          P+HW C +G+   VELL+E++      +    +PL  A+ N+N+   E+L
Sbjct:  699 DAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEV-FQKMEGNSFSPLHCAVINDNEGAAEML 826          

HSP 6 Score: 60.077 bits (144), Expect = 2.414e-10
Identity = 41/146 (28.08%), Postives = 67/146 (45.89%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+ HN  + A+D   + P+ +A   G +  V++L+     D+   DK     +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  821 GAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVLVSSAKADLTLQDKNKNTALHLACSKGHETSALLILEQITDRNLINATNSALQTPLHVAARNGLTVVVQELLG 966          

HSP 7 Score: 53.5286 bits (127), Expect = 3.680e-8
Identity = 36/110 (32.73%), Postives = 56/110 (50.91%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            R PIH A   GH+E ++LLI  N D++A     D   Q P+ L+   GH+E V  L+    +++A DK     +H    +G+ E VE L++   +   RD     P+ +A
Sbjct:  637 RTPIHSAAINGHSECLRLLI-GNADVQAAVDIHDGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLA 745          

HSP 8 Score: 51.9878 bits (123), Expect = 1.368e-7
Identity = 37/161 (22.98%), Postives = 62/161 (38.51%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNC-------------------------------------DMEALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   R PIH A   GH  ++  L++    ++ +    D     P+H ACY GH   V+LL+E                                         +++  + P+H    + +IE ++LL+     +NA D     PL +A +N     VE+L
Sbjct:  734 RDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEVFQKMEGNSFSPLHCAVINDNEGAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVL 894          

HSP 9 Score: 51.6026 bits (122), Expect = 1.977e-7
Identity = 31/117 (26.50%), Postives = 60/117 (51.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   + +  ++  D     PL +++ N + E V
Sbjct:  572 PLHLAAYHGHHQALEVLVQSLLDLDVRNSTGRTPLDLAAFKGHVECVDVLINQGASILVKDYVVKRTPIHSAAINGHSECLRLLIGNADVQAAVDIHDGNGQTPLMLSVLNGHTECV 688          

HSP 10 Score: 50.0618 bits (118), Expect = 6.608e-7
Identity = 37/144 (25.69%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+   ++  ++++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  189 DKKD---RRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELIDCGANVNQVNERGFTPLHFAAASTHGALCLELLVCNGADVNIKSKDGKTPL 329          

HSP 11 Score: 48.9062 bits (115), Expect = 1.364e-6
Identity = 33/126 (26.19%), Postives = 57/126 (45.24%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINA----RDKCEMNPLDIAIKNENKELVEIL 551
            D + + P+  +   GH E V  L+    +++A DK  +  +H     GH E V+ L++HN +    D   + PIH     G+I ++  L++    ++      D     PL  A  N +   VE+L
Sbjct:  669 DGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELL 794          

HSP 12 Score: 47.7506 bits (112), Expect = 3.729e-6
Identity = 32/118 (27.12%), Postives = 50/118 (42.37%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKE 536
            +D +   P+H A   GH  ++  LI    D          P+HLA   G S+  + L+    D++  D   +  +H     G +E + LL+    D N +DK    PL  A  N N +
Sbjct:  354 EDKNGNTPLHIAARYGHELLINTLITSRADTSKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTHDDFGRTCLHAAAAGGNLECLNLLLSTGADFNKKDKFGRTPLHYAAANCNYQ 471          
BLAST of SMED30016539 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 84.7297 bits (208), Expect = 6.502e-19
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V LL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +++ L++   D+N  +     PL +A  N
Sbjct:  145 RTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYN 253          

HSP 2 Score: 64.3142 bits (155), Expect = 8.195e-12
Identity = 43/152 (28.29%), Postives = 76/152 (50.00%), Query Frame = 3
Query:  108 EFKLFINAIRSGSVELFRRY-FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +F   + AI +G  +  R   FK+E    +D + R P+H A + G AEI++LLI     + A D +   P+H A      + V++L++H+ D+ A DK    P+H    +  ++  E L+    ++N  D+     L  A  + + E+V +L
Sbjct:   12 DFPPLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLL 163          

HSP 3 Score: 60.077 bits (144), Expect = 2.253e-10
Identity = 37/129 (28.68%), Postives = 63/129 (48.84%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK-------QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V+ L++HN +    D + + PIHLA   GH   +  L++    ++ +          P+HW C +G+   VELL+E++      +    +PL  A+ N+N+   E+L
Sbjct:  687 DAKDKWGRTALHRGVMTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEV-FQKMEGNSFSPLHCAVINDNEGAAEML 814          

HSP 4 Score: 51.9878 bits (123), Expect = 1.458e-7
Identity = 37/161 (22.98%), Postives = 62/161 (38.51%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNC-------------------------------------DMEALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   R PIH A   GH  ++  L++    ++ +    D     P+H ACY GH   V+LL+E                                         +++  + P+H    + +IE ++LL+     +NA D     PL +A +N     VE+L
Sbjct:  722 RDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEVFQKMEGNSFSPLHCAVINDNEGAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVL 882          

HSP 5 Score: 49.6766 bits (117), Expect = 7.664e-7
Identity = 37/144 (25.69%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+   ++  ++++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  174 DKKD---RRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELIDCGANVNQVNERGFTPLHFAAASTHGALCLELLVCNGADVNIKSKDGKTPL 314          

HSP 6 Score: 48.9062 bits (115), Expect = 1.567e-6
Identity = 33/126 (26.19%), Postives = 57/126 (45.24%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINA----RDKCEMNPLDIAIKNENKELVEIL 551
            D + + P+  +   GH E V  L+    +++A DK  +  +H     GH E V+ L++HN +    D   + PIH     G+I ++  L++    ++      D     PL  A  N +   VE+L
Sbjct:  657 DGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGVMTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELL 782          

HSP 7 Score: 47.3654 bits (111), Expect = 5.893e-6
Identity = 32/124 (25.81%), Postives = 54/124 (43.55%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECE-----SGYIEIVELLIERKCDINARD 485
            G+ E+             D   R P+H A F  H E ++LL+ HN  + A+D   + P+ +A   G +  V++L+     D+   DK     +H  C      SG   ++ +L  R   + AR+
Sbjct:  809 GAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVLVSSAKADLTLQDKNKNTALHLACSKVCTLSGKYLLLGILSCRPLHVAARN 932          
BLAST of SMED30016539 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 84.7297 bits (208), Expect = 6.568e-19
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V LL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +++ L++   D+N  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYN 249          

HSP 2 Score: 63.1586 bits (152), Expect = 1.933e-11
Identity = 39/116 (33.62%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+  A F G  + V+ LI    D+   D +K+ P+H A Y G +E ++LLI     + A D +   P+H    S   + V++L++   D+NARDK    PL IA  N+  +  E L
Sbjct:   11 PLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEAL 126          

HSP 3 Score: 62.7734 bits (151), Expect = 2.699e-11
Identity = 42/148 (28.38%), Postives = 74/148 (50.00%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRY-FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
             + AI +G  +  R   FK+E    +D + R P+H A + G AEI++LLI     + A D +   P+H A      + V++L++H+ D+ A DK    P+H    +  ++  E L+    ++N  D+     L  A  + + E+V +L
Sbjct:   12 LVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLL 159          

HSP 4 Score: 62.3882 bits (150), Expect = 4.263e-11
Identity = 39/144 (27.08%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   + V++L++H+ D+ A DK  Q P+H+A                                  + GH E V LL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   70 KDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAA 213          

HSP 5 Score: 60.077 bits (144), Expect = 2.599e-10
Identity = 37/129 (28.68%), Postives = 63/129 (48.84%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK-------QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V+ L++HN +    D + + PIHLA   GH   +  L++    ++ +          P+HW C +G+   VELL+E++      +    +PL  A+ N+N+   E+L
Sbjct:  680 DAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEV-FQKMEGNSFSPLHCAVINDNEGAAEML 807          

HSP 6 Score: 60.077 bits (144), Expect = 2.700e-10
Identity = 41/146 (28.08%), Postives = 67/146 (45.89%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+ HN  + A+D   + P+ +A   G +  V++L+     D+   DK     +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  802 GAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVLVSSAKADLTLQDKNKNTALHLACSKGHETSALLILEQITDRNLINATNSALQTPLHVAARNGLTVVVQELLG 947          

HSP 7 Score: 53.5286 bits (127), Expect = 3.886e-8
Identity = 36/110 (32.73%), Postives = 56/110 (50.91%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            R PIH A   GH+E ++LLI  N D++A     D   Q P+ L+   GH+E V  L+    +++A DK     +H    +G+ E VE L++   +   RD     P+ +A
Sbjct:  618 RTPIHSAAINGHSECLRLLI-GNADVQAAVDIHDGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLA 726          

HSP 8 Score: 51.9878 bits (123), Expect = 1.445e-7
Identity = 37/161 (22.98%), Postives = 62/161 (38.51%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNC-------------------------------------DMEALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   R PIH A   GH  ++  L++    ++ +    D     P+H ACY GH   V+LL+E                                         +++  + P+H    + +IE ++LL+     +NA D     PL +A +N     VE+L
Sbjct:  715 RDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEVFQKMEGNSFSPLHCAVINDNEGAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVL 875          

HSP 9 Score: 51.6026 bits (122), Expect = 2.108e-7
Identity = 31/117 (26.50%), Postives = 60/117 (51.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   + +  ++  D     PL +++ N + E V
Sbjct:  553 PLHLAAYHGHHQALEVLVQSLLDLDVRNSTGRTPLDLAAFKGHVECVDVLINQGASILVKDYVVKRTPIHSAAINGHSECLRLLIGNADVQAAVDIHDGNGQTPLMLSVLNGHTECV 669          

HSP 10 Score: 49.6766 bits (117), Expect = 7.740e-7
Identity = 37/144 (25.69%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+   ++  ++++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  170 DKKD---RRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELIDCGANVNQVNERGFTPLHFAAASTHGALCLELLVCNGADVNIKSKDGKTPL 310          

HSP 11 Score: 48.9062 bits (115), Expect = 1.454e-6
Identity = 33/126 (26.19%), Postives = 57/126 (45.24%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINA----RDKCEMNPLDIAIKNENKELVEIL 551
            D + + P+  +   GH E V  L+    +++A DK  +  +H     GH E V+ L++HN +    D   + PIH     G+I ++  L++    ++      D     PL  A  N +   VE+L
Sbjct:  650 DGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELL 775          

HSP 12 Score: 47.7506 bits (112), Expect = 3.975e-6
Identity = 32/118 (27.12%), Postives = 50/118 (42.37%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKE 536
            +D +   P+H A   GH  ++  LI    D          P+HLA   G S+  + L+    D++  D   +  +H     G +E + LL+    D N +DK    PL  A  N N +
Sbjct:  335 EDKNGNTPLHIAARYGHELLINTLITSRADTSKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTHDDFGRTCLHAAAAGGNLECLNLLLSTGADFNKKDKFGRTPLHYAAANCNYQ 452          
BLAST of SMED30016539 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 84.7297 bits (208), Expect = 6.589e-19
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V LL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +++ L++   D+N  +     PL +A  N
Sbjct:  168 RTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYN 276          

HSP 2 Score: 63.1586 bits (152), Expect = 1.939e-11
Identity = 39/116 (33.62%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+  A F G  + V+ LI    D+   D +K+ P+H A Y G +E ++LLI     + A D +   P+H    S   + V++L++   D+NARDK    PL IA  N+  +  E L
Sbjct:   38 PLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEAL 153          

HSP 3 Score: 62.7734 bits (151), Expect = 2.707e-11
Identity = 42/148 (28.38%), Postives = 74/148 (50.00%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRY-FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
             + AI +G  +  R   FK+E    +D + R P+H A + G AEI++LLI     + A D +   P+H A      + V++L++H+ D+ A DK    P+H    +  ++  E L+    ++N  D+     L  A  + + E+V +L
Sbjct:   39 LVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLL 186          

HSP 4 Score: 62.3882 bits (150), Expect = 4.276e-11
Identity = 39/144 (27.08%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   + V++L++H+ D+ A DK  Q P+H+A                                  + GH E V LL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   97 KDSKWLTPLHRAVASCSEDAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAA 240          

HSP 5 Score: 60.077 bits (144), Expect = 2.607e-10
Identity = 37/129 (28.68%), Postives = 63/129 (48.84%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK-------QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V+ L++HN +    D + + PIHLA   GH   +  L++    ++ +          P+HW C +G+   VELL+E++      +    +PL  A+ N+N+   E+L
Sbjct:  707 DAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEV-FQKMEGNSFSPLHCAVINDNEGAAEML 834          

HSP 6 Score: 60.077 bits (144), Expect = 2.708e-10
Identity = 41/146 (28.08%), Postives = 67/146 (45.89%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+ HN  + A+D   + P+ +A   G +  V++L+     D+   DK     +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  829 GAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVLVSSAKADLTLQDKNKNTALHLACSKGHETSALLILEQITDRNLINATNSALQTPLHVAARNGLTVVVQELLG 974          

HSP 7 Score: 53.5286 bits (127), Expect = 3.898e-8
Identity = 36/110 (32.73%), Postives = 56/110 (50.91%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            R PIH A   GH+E ++LLI  N D++A     D   Q P+ L+   GH+E V  L+    +++A DK     +H    +G+ E VE L++   +   RD     P+ +A
Sbjct:  645 RTPIHSAAINGHSECLRLLI-GNADVQAAVDIHDGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLA 753          

HSP 8 Score: 51.9878 bits (123), Expect = 1.449e-7
Identity = 37/161 (22.98%), Postives = 62/161 (38.51%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEHNC-------------------------------------DMEALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   R PIH A   GH  ++  L++    ++ +    D     P+H ACY GH   V+LL+E                                         +++  + P+H    + +IE ++LL+     +NA D     PL +A +N     VE+L
Sbjct:  742 RDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLEQEVFQKMEGNSFSPLHCAVINDNEGAAEMLIDTLGTSIVNSVDSKGRTPLHAAAFTDHIECLQLLLSHNAQVNAVDSTGKTPLMMAAENGQTSAVEVL 902          

HSP 9 Score: 51.6026 bits (122), Expect = 2.114e-7
Identity = 31/117 (26.50%), Postives = 60/117 (51.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   + +  ++  D     PL +++ N + E V
Sbjct:  580 PLHLAAYHGHHQALEVLVQSLLDLDVRNSTGRTPLDLAAFKGHVECVDVLINQGASILVKDYVVKRTPIHSAAINGHSECLRLLIGNADVQAAVDIHDGNGQTPLMLSVLNGHTECV 696          

HSP 10 Score: 49.6766 bits (117), Expect = 7.762e-7
Identity = 37/144 (25.69%), Postives = 62/144 (43.06%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+   ++  ++++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  197 DKKD---RRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELIDCGANVNQVNERGFTPLHFAAASTHGALCLELLVCNGADVNIKSKDGKTPL 337          

HSP 11 Score: 48.9062 bits (115), Expect = 1.458e-6
Identity = 33/126 (26.19%), Postives = 57/126 (45.24%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINA----RDKCEMNPLDIAIKNENKELVEIL 551
            D + + P+  +   GH E V  L+    +++A DK  +  +H     GH E V+ L++HN +    D   + PIH     G+I ++  L++    ++      D     PL  A  N +   VE+L
Sbjct:  677 DGNGQTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELL 802          

HSP 12 Score: 47.7506 bits (112), Expect = 3.986e-6
Identity = 32/118 (27.12%), Postives = 50/118 (42.37%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKE 536
            +D +   P+H A   GH  ++  LI    D          P+HLA   G S+  + L+    D++  D   +  +H     G +E + LL+    D N +DK    PL  A  N N +
Sbjct:  362 EDKNGNTPLHIAARYGHELLINTLITSRADTSKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTHDDFGRTCLHAAAAGGNLECLNLLLSTGADFNKKDKFGRTPLHYAAANCNYQ 479          
BLAST of SMED30016539 vs. Ensembl Mouse
Match: Ankrd28 (ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MGI:2145661])

HSP 1 Score: 88.9669 bits (219), Expect = 1.736e-20
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+VKLL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +V+ L++   D+N  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYN 249          

HSP 2 Score: 68.9366 bits (167), Expect = 1.603e-13
Identity = 41/144 (28.47%), Postives = 63/144 (43.75%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E VKLL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   70 KDSKWLTPLHRAVASCSEEAVQILLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAA 213          

HSP 3 Score: 56.9954 bits (136), Expect = 1.852e-9
Identity = 39/146 (26.71%), Postives = 66/146 (45.21%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+  N  + + D   + P+ +A   G +  V++L+   + D+   DK     +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  802 GAAEMLIDSLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSQNAQVNSADSTGKTPLMMAAENGQTNTVEMLVSSASADLTLQDKSKNTALHLACGKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLG 947          

HSP 4 Score: 55.4546 bits (132), Expect = 7.322e-9
Identity = 40/133 (30.08%), Postives = 63/133 (47.37%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA----LDKQ---PIHWECESGYIEIVELLIE----RKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V  L++H       D + + PIHL+   GH   +  L++    ++A    +D      +HW C +G+   VELL+E    +K D NA      +PL  A+ N+N+   E+L
Sbjct:  680 DAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSATSVDANPAVVDNHGYTALHWACYNGHETCVELLLEQDVFQKIDGNA-----FSPLHCAVINDNEGAAEML 807          

HSP 5 Score: 53.5286 bits (127), Expect = 2.888e-8
Identity = 31/117 (26.50%), Postives = 61/117 (52.14%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDM----EALDKQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +      L + PIH    +G+ E + LLI   E +  ++ +D     PL +++ N + + V
Sbjct:  553 PLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYVLKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCV 669          

HSP 6 Score: 51.2174 bits (121), Expect = 1.738e-7
Identity = 37/144 (25.69%), Postives = 61/144 (42.36%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+   ++   +++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  170 DKKD---RRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPL 310          

HSP 7 Score: 47.7506 bits (112), Expect = 3.276e-6
Identity = 34/115 (29.57%), Postives = 57/115 (49.57%), Query Frame = 3
Query:  192 KDVDLRR-PIHHACFAGHAEIVKLLI---EHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD  L+R PIH A   GH+E ++LLI   E    ++  D   Q P+ L+   GH++ V  L+    +++A DK     +H    +G+ E V+ L++       RD     P+ ++
Sbjct:  612 KDYVLKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLS 726          
BLAST of SMED30016539 vs. Ensembl Mouse
Match: Ankrd28 (ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MGI:2145661])

HSP 1 Score: 88.9669 bits (219), Expect = 2.112e-20
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+VKLL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +V+ L++   D+N  +     PL +A  N
Sbjct:  171 RTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYN 279          

HSP 2 Score: 68.5514 bits (166), Expect = 2.082e-13
Identity = 41/144 (28.47%), Postives = 63/144 (43.75%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E VKLL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:  100 KDSKWLTPLHRAVASCSEEAVQILLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAA 243          

HSP 3 Score: 56.9954 bits (136), Expect = 2.289e-9
Identity = 39/146 (26.71%), Postives = 66/146 (45.21%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+  N  + + D   + P+ +A   G +  V++L+   + D+   DK     +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  832 GAAEMLIDSLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSQNAQVNSADSTGKTPLMMAAENGQTNTVEMLVSSASADLTLQDKSKNTALHLACGKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLG 977          

HSP 4 Score: 55.0694 bits (131), Expect = 8.878e-9
Identity = 40/133 (30.08%), Postives = 63/133 (47.37%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA----LDKQ---PIHWECESGYIEIVELLIE----RKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V  L++H       D + + PIHL+   GH   +  L++    ++A    +D      +HW C +G+   VELL+E    +K D NA      +PL  A+ N+N+   E+L
Sbjct:  710 DAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSATSVDANPAVVDNHGYTALHWACYNGHETCVELLLEQDVFQKIDGNA-----FSPLHCAVINDNEGAAEML 837          

HSP 5 Score: 53.5286 bits (127), Expect = 3.308e-8
Identity = 31/117 (26.50%), Postives = 61/117 (52.14%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDM----EALDKQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +      L + PIH    +G+ E + LLI   E +  ++ +D     PL +++ N + + V
Sbjct:  583 PLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYVLKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCV 699          

HSP 6 Score: 51.2174 bits (121), Expect = 2.027e-7
Identity = 37/144 (25.69%), Postives = 61/144 (42.36%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+   ++   +++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  200 DKKD---RRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPL 340          

HSP 7 Score: 47.3654 bits (111), Expect = 3.891e-6
Identity = 34/115 (29.57%), Postives = 57/115 (49.57%), Query Frame = 3
Query:  192 KDVDLRR-PIHHACFAGHAEIVKLLI---EHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD  L+R PIH A   GH+E ++LLI   E    ++  D   Q P+ L+   GH++ V  L+    +++A DK     +H    +G+ E V+ L++       RD     P+ ++
Sbjct:  642 KDYVLKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLS 756          
BLAST of SMED30016539 vs. Ensembl Mouse
Match: Gabpb1 (GA repeat binding protein, beta 1 [Source:MGI Symbol;Acc:MGI:95611])

HSP 1 Score: 77.0258 bits (188), Expect = 3.751e-17
Identity = 45/149 (30.20%), Postives = 73/149 (48.99%), Query Frame = 3
Query:  114 KLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            K  + A R+G  +  R           D     P+H A   GH    ++L+      +A  K  + P+H+A  +GH+  V++L++H  D+ A   L    +HW  E  + E+VELLI+   D++ + K      DI+I N N++L EIL
Sbjct:    8 KKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLAAQYGHFSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHANIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELLIKYGADVHTQSKFCKTAFDISIDNGNEDLAEIL 156          

HSP 2 Score: 65.4698 bits (158), Expect = 8.750e-13
Identity = 36/121 (29.75%), Postives = 64/121 (52.89%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VDL + +  A  AG  + V++L+ +       D     P+HLA   GH    ++L+      +A   +D+ P+H     G+  IVE+L++   D+NA+D  +M  L  A ++ ++E+VE+L
Sbjct:    4 VDLGKKLLEAARAGQDDEVRILMANGAPFTT-DWLGTSPLHLAAQYGHFSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHANIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELL 123          
BLAST of SMED30016539 vs. Ensembl Mouse
Match: Gabpb1 (GA repeat binding protein, beta 1 [Source:MGI Symbol;Acc:MGI:95611])

HSP 1 Score: 75.8702 bits (185), Expect = 3.443e-16
Identity = 45/149 (30.20%), Postives = 73/149 (48.99%), Query Frame = 3
Query:  114 KLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            K  + A R+G  +  R           D     P+H A   GH    ++L+      +A  K  + P+H+A  +GH+  V++L++H  D+ A   L    +HW  E  + E+VELLI+   D++ + K      DI+I N N++L EIL
Sbjct:    8 KKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLAAQYGHFSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHANIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELLIKYGADVHTQSKFCKTAFDISIDNGNEDLAEIL 156          

HSP 2 Score: 63.5438 bits (153), Expect = 8.441e-12
Identity = 36/121 (29.75%), Postives = 64/121 (52.89%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VDL + +  A  AG  + V++L+ +       D     P+HLA   GH    ++L+      +A   +D+ P+H     G+  IVE+L++   D+NA+D  +M  L  A ++ ++E+VE+L
Sbjct:    4 VDLGKKLLEAARAGQDDEVRILMANGAPFTT-DWLGTSPLHLAAQYGHFSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHANIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELL 123          
BLAST of SMED30016539 vs. Ensembl Mouse
Match: Gabpb1 (GA repeat binding protein, beta 1 [Source:MGI Symbol;Acc:MGI:95611])

HSP 1 Score: 75.8702 bits (185), Expect = 3.902e-16
Identity = 45/149 (30.20%), Postives = 73/149 (48.99%), Query Frame = 3
Query:  114 KLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            K  + A R+G  +  R           D     P+H A   GH    ++L+      +A  K  + P+H+A  +GH+  V++L++H  D+ A   L    +HW  E  + E+VELLI+   D++ + K      DI+I N N++L EIL
Sbjct:    8 KKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLAAQYGHFSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHANIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELLIKYGADVHTQSKFCKTAFDISIDNGNEDLAEIL 156          

HSP 2 Score: 63.5438 bits (153), Expect = 8.801e-12
Identity = 36/121 (29.75%), Postives = 64/121 (52.89%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VDL + +  A  AG  + V++L+ +       D     P+HLA   GH    ++L+      +A   +D+ P+H     G+  IVE+L++   D+NA+D  +M  L  A ++ ++E+VE+L
Sbjct:    4 VDLGKKLLEAARAGQDDEVRILMANGAPFTT-DWLGTSPLHLAAQYGHFSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHANIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELL 123          
BLAST of SMED30016539 vs. UniProt/SwissProt
Match: sp|Q505D1|ANR28_MOUSE (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A OS=Mus musculus OX=10090 GN=Ankrd28 PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.214e-19
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+VKLL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +V+ L++   D+N  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYN 249          

HSP 2 Score: 68.9366 bits (167), Expect = 1.122e-12
Identity = 41/144 (28.47%), Postives = 63/144 (43.75%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E VKLL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   70 KDSKWLTPLHRAVASCSEEAVQILLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAA 213          

HSP 3 Score: 56.9954 bits (136), Expect = 1.295e-8
Identity = 39/146 (26.71%), Postives = 66/146 (45.21%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+  N  + + D   + P+ +A   G +  V++L+   + D+   DK     +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  802 GAAEMLIDSLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSQNAQVNSADSTGKTPLMMAAENGQTNTVEMLVSSASADLTLQDKSKNTALHLACGKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLG 947          

HSP 4 Score: 55.4546 bits (132), Expect = 5.122e-8
Identity = 40/133 (30.08%), Postives = 63/133 (47.37%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA----LDKQ---PIHWECESGYIEIVELLIE----RKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V  L++H       D + + PIHL+   GH   +  L++    ++A    +D      +HW C +G+   VELL+E    +K D NA      +PL  A+ N+N+   E+L
Sbjct:  680 DAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSATSVDANPAVVDNHGYTALHWACYNGHETCVELLLEQDVFQKIDGNA-----FSPLHCAVINDNEGAAEML 807          

HSP 5 Score: 53.5286 bits (127), Expect = 2.020e-7
Identity = 31/117 (26.50%), Postives = 61/117 (52.14%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDM----EALDKQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +      L + PIH    +G+ E + LLI   E +  ++ +D     PL +++ N + + V
Sbjct:  553 PLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYVLKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCV 669          

HSP 6 Score: 51.2174 bits (121), Expect = 1.216e-6
Identity = 37/144 (25.69%), Postives = 61/144 (42.36%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+   ++   +++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  170 DKKD---RRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPL 310          
BLAST of SMED30016539 vs. UniProt/SwissProt
Match: sp|O15084|ANR28_HUMAN (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A OS=Homo sapiens OX=9606 GN=ANKRD28 PE=1 SV=5)

HSP 1 Score: 88.9669 bits (219), Expect = 1.350e-19
Identity = 44/109 (40.37%), Postives = 63/109 (57.80%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+VKLL+    +I A DK+ +R IH A Y GH E VKLL+ H  ++   DK+   P+H    SG I +V+ L++   D+N  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYN 249          

HSP 2 Score: 68.5514 bits (166), Expect = 1.659e-12
Identity = 41/144 (28.47%), Postives = 63/144 (43.75%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E VKLL+    ++ A DK+    IHW    G+IE+V+LL+    ++  +DK    PL  A
Sbjct:   70 KDSKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAA 213          

HSP 3 Score: 58.151 bits (139), Expect = 6.541e-9
Identity = 38/146 (26.03%), Postives = 67/146 (45.89%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            G+ E+             D   R P+H A F  H E ++LL+ HN  + ++D   + P+ +A   G +  V++L+   + ++   D      +H  C  G+     L++E+  D   INA +     PL +A +N    +V E+LG
Sbjct:  802 GAAEMLIDTLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSHNAQVNSVDSTGKTPLMMAAENGQTNTVEMLVSSASAELTLQDNSKNTALHLACSKGHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLG 947          

HSP 4 Score: 55.0694 bits (131), Expect = 5.848e-8
Identity = 37/129 (28.68%), Postives = 60/129 (46.51%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA----LDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + KD   R  +H     GH E V  L++H       D + + PIHL+   GH   +  L++    M+A     D      +HW C +G+   VELL+E++      +    +PL  A+ N+N+   E+L
Sbjct:  680 DAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPATADNHGYTALHWACYNGHETCVELLLEQEV-FQKTEGNAFSPLHCAVINDNEGAAEML 807          

HSP 5 Score: 53.1434 bits (126), Expect = 2.760e-7
Identity = 31/117 (26.50%), Postives = 61/117 (52.14%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A + GH + +++L++   D++  +   + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   E +  ++ +D     PL +++ N + + V
Sbjct:  553 PLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCV 669          

HSP 6 Score: 49.2914 bits (116), Expect = 5.594e-6
Identity = 37/144 (25.69%), Postives = 60/144 (41.67%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQK---------------------------------QRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            ++KD   RR IH A + GH E+VKLL+ H  ++   DK+                                    P+H+ACY G    V  LI+    +   +++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  170 DKKD---RRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGAIVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPL 310          
BLAST of SMED30016539 vs. UniProt/SwissProt
Match: sp|Q9ULJ7|ANR50_HUMAN (Ankyrin repeat domain-containing protein 50 OS=Homo sapiens OX=9606 GN=ANKRD50 PE=1 SV=4)

HSP 1 Score: 77.411 bits (189), Expect = 1.596e-15
Identity = 42/134 (31.34%), Postives = 77/134 (57.46%), Query Frame = 3
Query:  165 YFKEEFS--ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQPIHWEC---ESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            YF E  +  E  D + R  +H +C+ GH E+V++LI ++ D+ A D +K+  +  A +QGH + V+LLIEH   ++    Q     C   + G+I++V++L+E   D N  D+     + +A KN + +++++L
Sbjct:  964 YFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLL 1097          

HSP 2 Score: 58.9214 bits (141), Expect = 3.571e-9
Identity = 40/153 (26.14%), Postives = 77/153 (50.33%), Query Frame = 3
Query:  126 NAIRSGSVELFRRYFKEEFSER-----KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK------QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            NA+R  ++E  R   +  FS       KD D R  ++         + +  +E+  ++EA D + +  +H++C+QGH E V++LI ++ D+ A D       Q   W+   G++++V+LLIE    ++         L IA +  + ++V++L
Sbjct:  915 NALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQ---GHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVL 1064          

HSP 3 Score: 58.151 bits (139), Expect = 6.557e-9
Identity = 35/127 (27.56%), Postives = 63/127 (49.61%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--------ALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            D D R  +  A + GH +IV  L++H  ++   D + +  +  A Y GH E V+ L++H  ++         AL    +      G+  +V LLI+R  +++  DK  M PL +A    + ++V++L
Sbjct:  641 DADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLL 767          

HSP 4 Score: 56.225 bits (134), Expect = 2.405e-8
Identity = 41/135 (30.37%), Postives = 64/135 (47.41%), Query Frame = 3
Query:   96 SMDEEFKLFIN-AIRSGSVELFRRYFKEEFSE-RKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH--NCDMEALD-KQPIHWECESGYIEIVELLIERKCDINARD 485
            S+D E +  ++ A   G+VE+ R        E  +D     P+H A F GH  I + LIE       +D   + P  LA  +GH + V++L+E+  N D    D +  +      G+ +IVELL     D+N +D
Sbjct:  809 SIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKD 943          

HSP 5 Score: 52.7582 bits (125), Expect = 3.996e-7
Identity = 37/128 (28.91%), Postives = 60/128 (46.88%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFA-----GHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +DVD R  +  A        GHA +V LLI+   +++  DK    P+ +A Y+GH + V LL+E   D++  D   + P+      G+  +V  L+     +++ D      L IA    N E+V  L
Sbjct:  706 EDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTL 833          
BLAST of SMED30016539 vs. UniProt/SwissProt
Match: sp|Q1RMI3|GABP1_BOVIN (GA-binding protein subunit beta-1 OS=Bos taurus OX=9913 GN=GABPB1 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.293e-15
Identity = 45/149 (30.20%), Postives = 73/149 (48.99%), Query Frame = 3
Query:  114 KLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            K  + A R+G  +  R           D     P+H A   GH    ++L+      +A  K  + P+H+A  +GH+  V++L++H  D+ A   L    +HW  E  + E+VELLI+   D++ + K      DI+I N N++L EIL
Sbjct:    8 KKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLAAQYGHYSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHASIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELLIKYGADVHTQSKFCKTAFDISIDNGNEDLAEIL 156          

HSP 2 Score: 64.6994 bits (156), Expect = 2.511e-11
Identity = 36/121 (29.75%), Postives = 64/121 (52.89%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VDL + +  A  AG  + V++L+ +       D     P+HLA   GH    ++L+      +A   +D+ P+H     G+  IVE+L++   D+NA+D  +M  L  A ++ ++E+VE+L
Sbjct:    4 VDLGKKLLEAARAGQDDEVRILMANGAPF-TTDWLGTSPLHLAAQYGHYSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHASIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELL 123          
BLAST of SMED30016539 vs. UniProt/SwissProt
Match: sp|Q06547|GABP1_HUMAN (GA-binding protein subunit beta-1 OS=Homo sapiens OX=9606 GN=GABPB1 PE=1 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 2.676e-15
Identity = 45/149 (30.20%), Postives = 73/149 (48.99%), Query Frame = 3
Query:  114 KLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            K  + A R+G  +  R           D     P+H A   GH    ++L+      +A  K  + P+H+A  +GH+  V++L++H  D+ A   L    +HW  E  + E+VELLI+   D++ + K      DI+I N N++L EIL
Sbjct:    8 KKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLAAQYGHYSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHASIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELLIKYGADVHTQSKFCKTAFDISIDNGNEDLAEIL 156          

HSP 2 Score: 64.6994 bits (156), Expect = 2.642e-11
Identity = 36/121 (29.75%), Postives = 64/121 (52.89%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            VDL + +  A  AG  + V++L+ +       D     P+HLA   GH    ++L+      +A   +D+ P+H     G+  IVE+L++   D+NA+D  +M  L  A ++ ++E+VE+L
Sbjct:    4 VDLGKKLLEAARAGQDDEVRILMANGAPF-TTDWLGTSPLHLAAQYGHYSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHASIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELL 123          
BLAST of SMED30016539 vs. TrEMBL
Match: V4K3J3 (Ankyrin repeat OS=uncultured Acidilobus sp. JCHS OX=1410576 GN=JCHSAcid_12510 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.027e-23
Identity = 48/116 (41.38%), Postives = 70/116 (60.34%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GHAE+  LLIE+  D  A D++ + P+H A  +GH+E VK+L+    D+ A D     P+HW  E G+ E+V LL++R  D NARD C + PLD A    ++ +V +L
Sbjct:  105 PLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADRGHAEVVKVLLREGADVNARDDHGVTPLHWAAERGHAEVVLLLLQRGADPNARDNCGLTPLDRAAAGGHEGVVRML 220          

HSP 2 Score: 85.1149 bits (209), Expect = 9.753e-17
Identity = 41/116 (35.34%), Postives = 66/116 (56.90%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A + G A+ V+ L++   D  A D     P+H A  +GH+E   LLIE+  D  A D++   P+HW  + G+ E+V++L+    D+NARD   + PL  A +  + E+V +L
Sbjct:   72 PLHWAAWGGRADNVRALLKAGADPNARDDNGFTPLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADRGHAEVVKVLLREGADVNARDDHGVTPLHWAAERGHAEVVLLL 187          

HSP 3 Score: 73.1738 bits (178), Expect = 2.775e-12
Identity = 43/154 (27.92%), Postives = 68/154 (44.16%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            M  + +    AI+ G         K      +D     P+H A   G  E+VK L++   D    +     P+H A + G ++ V+ L++   D  A D     P+H   + G+ E+  LLIE   D NARD+    PL  A    + E+V++L
Sbjct:    1 MGADVEALAEAIKGGDAAKALALLKSVDPSSRDRFGNTPLHWAAAYGPEELVKSLLDMRADPNVRNSNNVTPLHWAAWGGRADNVRALLKAGADPNARDDNGFTPLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADRGHAEVVKVL 154          
BLAST of SMED30016539 vs. TrEMBL
Match: V4JNT4 (Ankyrin repeat OS=uncultured Acidilobus sp. OSP8 OX=1410575 GN=OSP8Acid_01540 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.963e-23
Identity = 47/116 (40.52%), Postives = 71/116 (61.21%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GHAE+  LLIE+  D  A D++ + P+H A  +GH+E VK+L+    D+ A D     P+HW  E G+ E+V LL++R  D NA+D C + PLD A    ++ +V++L
Sbjct:  105 PLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADKGHAEVVKVLLREGADINARDDHSVTPLHWAAERGHAEVVWLLLQRGADPNAKDNCGLTPLDRAAAGGHEGIVKML 220          

HSP 2 Score: 87.0409 bits (214), Expect = 1.631e-17
Identity = 43/123 (34.96%), Postives = 69/123 (56.10%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            ++ D   P+H A + G A+ V+ L++   D  A D     P+H A  +GH+E   LLIE+  D  A D++   P+HW  + G+ E+V++L+    DINARD   + PL  A +  + E+V +L
Sbjct:   65 RNSDGVTPLHWAAWGGRADNVRALLKAGADPNARDDNGVTPLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADKGHAEVVKVLLREGADINARDDHSVTPLHWAAERGHAEVVWLL 187          

HSP 3 Score: 74.3294 bits (181), Expect = 1.014e-12
Identity = 43/154 (27.92%), Postives = 69/154 (44.81%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            M  + +  + A+R G         K      +D     P+H A   G  E+VK L++   D    +     P+H A + G ++ V+ L++   D  A D     P+H   + G+ E+  LLIE   D NARD+    PL  A    + E+V++L
Sbjct:    1 MGADVEALVEAMRRGDAAKALALLKSVDPSSRDGLGNTPLHWAAAYGPEELVKSLLDMRADPNVRNSDGVTPLHWAAWGGRADNVRALLKAGADPNARDDNGVTPLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADKGHAEVVKVL 154          
BLAST of SMED30016539 vs. TrEMBL
Match: V4JZM1 (Ankyrin repeat OS=uncultured Acidilobus sp. MG OX=1410573 GN=MGAcid_05560 PE=4 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.324e-22
Identity = 46/116 (39.66%), Postives = 69/116 (59.48%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GHAE+  LLIE+  D  A D++ + P+H A  +GH+E VK+L+    D+ A D     P+HW  E G+ E+V LL++R  D  A+D C + PLD A    ++ +V +L
Sbjct:  105 PLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADKGHAEVVKVLLREGADVNARDDHGVTPLHWAAERGHAEVVWLLLQRGADPKAKDNCGLTPLDRAAAGGHEGVVRML 220          

HSP 2 Score: 86.2705 bits (212), Expect = 3.576e-17
Identity = 42/123 (34.15%), Postives = 69/123 (56.10%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            ++ D   P+H A + G A+ V+ L++   D  A D     P+H A  +GH+E   LLIE+  D  A D++   P+HW  + G+ E+V++L+    D+NARD   + PL  A +  + E+V +L
Sbjct:   65 RNSDGVTPLHWAAWGGRADNVRALLKAGADPNARDDNGFTPLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADKGHAEVVKVLLREGADVNARDDHGVTPLHWAAERGHAEVVWLL 187          

HSP 3 Score: 75.485 bits (184), Expect = 3.104e-13
Identity = 45/154 (29.22%), Postives = 69/154 (44.81%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            M  + +    AIR G         K      +D     P+H A   G  E+VK L++   D  A +     P+H A + G ++ V+ L++   D  A D     P+H   + G+ E+  LLIE   D NARD+    PL  A    + E+V++L
Sbjct:    1 MGADVEALAEAIRRGDATKALALLKSVDPSSRDRFGNTPLHWAAAYGPEELVKSLLDMRADPNARNSDGVTPLHWAAWGGRADNVRALLKAGADPNARDDNGFTPLHRAADRGHAEVAWLLIENGADPNARDREGRTPLHWAADKGHAEVVKVL 154          

HSP 4 Score: 63.929 bits (154), Expect = 7.117e-9
Identity = 32/107 (29.91%), Postives = 54/107 (50.47%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNP 503
            +D + R P+H A   GHAE+VK+L+    D+ A D     P+H A  +GH+E V LL++   D +A D     P+      G+  +V +L++       + +  + P
Sbjct:  131 RDREGRTPLHWAADKGHAEVVKVLLREGADVNARDDHGVTPLHWAAERGHAEVVWLLLQRGADPKAKDNCGLTPLDRAAAGGHEGVVRMLLDAGASPQRQGQVGLEP 237          
BLAST of SMED30016539 vs. TrEMBL
Match: A0A1V4WVK3 (Ankyrin repeat protein OS=Syntrophorhabdus sp. PtaB.Bin047 OX=1811700 GN=A4E60_01960 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 2.972e-22
Identity = 55/145 (37.93%), Postives = 82/145 (56.55%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIK 521
            M++E ++FI AI  GSV+  R   +       ++   R P+H AC  GH +IV+LLIE   D+     +   P+H AC QGH +  +LL+ H  D  A  ++   P+HW CE  + ++V LLIE   D+ ARD  +  PLD+A+K
Sbjct:    1 MNQE-QIFI-AIDHGSVDAVREVLEAGADVNARNEHGRTPLHWACGQGHTDIVRLLIEAGADVNERSWKMVTPLHEACGQGHPDVARLLLSHGADPNARHRRGHTPLHWACEQRHPDVVRLLIEHGADVEARDDYDHTPLDVAMK 143          

HSP 2 Score: 64.3142 bits (155), Expect = 1.805e-9
Identity = 33/108 (30.56%), Postives = 58/108 (53.70%), Query Frame = 3
Query:  237 GHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            G  + V+ ++E   D+ A ++  + P+H AC QGH++ V+LLIE   D+     +   P+H  C  G+ ++  LL+    D NAR +    PL  A +  + ++V +L
Sbjct:   13 GSVDAVREVLEAGADVNARNEHGRTPLHWACGQGHTDIVRLLIEAGADVNERSWKMVTPLHEACGQGHPDVARLLLSHGADPNARHRRGHTPLHWACEQRHPDVVRLL 120          
BLAST of SMED30016539 vs. TrEMBL
Match: A0A497XRJ5 (Ankyrin repeat protein OS=Hydrogenivirga caldilitoris OX=246264 GN=BCF55_0017 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 5.572e-22
Identity = 56/166 (33.73%), Postives = 87/166 (52.41%), Query Frame = 3
Query:   63 LFVLIGEIFS*SMDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +F++I  I S  +     LF  A   G++E  R+         K+ +    +H A  AGH +IVK L+E   D+ A D  K  P+H A   GH E V  L+EH   + A +     P+H    +G+++IV+LL+ER   +N R+   + PL +A  N +KE+VE+L
Sbjct:    1 MFLII-FILSVGLSFSTDLFKTA-EGGNLETLRKLVNSSNVNMKNHEGESLLHVAAEAGHTDIVKFLLEQGADVNARDNYKATPLHFAANNGHYEIVLSLLEHGAKVNARNLNGWTPLHLASRNGHLKIVKLLLERGAKVNVRNGAGLTPLHVAAMNGHKEVVELL 164          

HSP 2 Score: 74.7146 bits (182), Expect = 4.226e-13
Identity = 42/130 (32.31%), Postives = 66/130 (50.77%), Query Frame = 3
Query:  186 ERKDVDLR-----RPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +  DV+ R      P+H A   GH EIV  L+EH   + A +     P+HLA   GH + VKLL+E    +   +     P+H    +G+ E+VELL++   D+ ++D   +  LD A    + E+V +L
Sbjct:   68 QGADVNARDNYKATPLHFAANNGHYEIVLSLLEHGAKVNARNLNGWTPLHLASRNGHLKIVKLLLERGAKVNVRNGAGLTPLHVAAMNGHKEVVELLLKAGADVKSKDNAGLTALDFAKDYGHPEVVNLL 197          
BLAST of SMED30016539 vs. Ensembl Cavefish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 84.7297 bits (208), Expect = 4.960e-19
Identity = 40/125 (32.00%), Postives = 73/125 (58.40%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + K  D + P+H A   GH E+VKLL+EH  + ++       P+H+A  +GH+   ++L++ N     + K+   P+H  C+ G +++ ELL+ER  + NA  K  + PL +A+ + N ++V++L
Sbjct:  439 DAKAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLL 563          

HSP 2 Score: 69.3218 bits (168), Expect = 9.801e-14
Identity = 34/116 (29.31%), Postives = 64/116 (55.17%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC   H   + LL++H+  +EA+ +    P+H+A + GH   VK L++      A +   + P+H    +G+ E+ + L++    ++A+ K +  PL  A +  +KELV++L
Sbjct:  349 PLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLL 464          

HSP 3 Score: 58.5362 bits (140), Expect = 5.105e-10
Identity = 38/145 (26.21%), Postives = 71/145 (48.97%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--ALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R G+V + R         + +  D   P+H A   GH  ++++L++    I+A  K    PIH+A    H + V+ L+++N +++   LD   P+H     G+  + ++L+++    NAR      PL IA K  +   +++L
Sbjct:  221 AARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLL 365          

HSP 4 Score: 58.151 bits (139), Expect = 5.831e-10
Identity = 33/121 (27.27%), Postives = 61/121 (50.41%), Query Frame = 3
Query:  198 VDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   P+H A   GH  + K+L++      A       P+H+AC + H   + LL++H+  +EA+ +    P+H     G++ IV+ L++R    NA +     PL +A +  + E+ + L
Sbjct:  311 LDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFL 431          

HSP 5 Score: 53.5286 bits (127), Expect = 2.946e-8
Identity = 27/116 (23.28%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A    +  + +LL+    ++    K    P+H+A  +G+   V+LL++    ++A  K    P+H    +G++ ++E+L+++   I A+ K  ++P+ +A + ++ + V  L
Sbjct:  184 PLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQL 299          

HSP 6 Score: 50.8322 bits (120), Expect = 1.891e-7
Identity = 28/116 (24.14%), Postives = 58/116 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+  +V+LL++    I+A  K +  P+H A   GH   +++L++    ++A  K    PIH   +  +++ V  L++   +I+      + PL +A    +  + ++L
Sbjct:  217 PLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVL 332          

HSP 7 Score: 49.2914 bits (116), Expect = 7.136e-7
Identity = 26/115 (22.61%), Postives = 59/115 (51.30%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A   GH ++V  L+ H  D+E   K+    +H+A   G  + V  L+ +  ++ A  ++   P++   +  ++E+V+ L+E   + +   +    PL +A++  ++ +V +L
Sbjct:   24 LHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALL 138          

HSP 8 Score: 47.7506 bits (112), Expect = 2.586e-6
Identity = 27/116 (23.28%), Postives = 55/116 (47.41%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC  G  ++ +LL+E   +  A  K    P+H+A +  + + VKLL+       +  +    P+H   +   +E+   L++   + N+     + PL +A +    ++V +L
Sbjct:  514 PLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALL 629          
BLAST of SMED30016539 vs. Ensembl Cavefish
Match: gabpb1 (GA binding protein transcription factor subunit beta 1 [Source:ZFIN;Acc:ZDB-GENE-061027-46])

HSP 1 Score: 81.6481 bits (200), Expect = 2.668e-18
Identity = 47/149 (31.54%), Postives = 76/149 (51.01%), Query Frame = 3
Query:  114 KLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEA---LDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            K  + A RSG  +  R           D     P+H +   GH    ++L+      +A  K  + P+H+A  +GH+  V++L++H  D+ A   L    +HW  E G+ ++VELL+    D++A+ K   + LDIA+ N N+EL EIL
Sbjct:    8 KKLLEAARSGQDDEVRILMANGAPFTTDWLGTSPLHLSAQYGHYSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHARIVEVLLQHGADVNAKDMLKMTALHWAAEHGHRDVVELLLRFGVDVHAQSKFCKSALDIALDNNNEELAEIL 156          
BLAST of SMED30016539 vs. Ensembl Cavefish
Match: psmd10 (proteasome 26S subunit, non-ATPase 10 [Source:NCBI gene;Acc:103037594])

HSP 1 Score: 79.337 bits (194), Expect = 3.951e-18
Identity = 42/126 (33.33%), Postives = 64/126 (50.79%), Query Frame = 3
Query:  183 SERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + R D D R  +H AC AGHA IV+ L++   +++  D     P+H+A   G  E V+ LI     + ++++    P+H+       EI  LL+E   D NA DK E  PL  A    N  L+++L
Sbjct:   33 ASRTDQDHRTALHWACSAGHANIVQFLLDVGAEVDLKDDASWTPLHIAASAGREEIVRSLISKGAQLNSVNQNGCTPLHYAASKNQYEIALLLLENGADPNATDKLESTPLHRASAKGNYRLIQLL 158          

HSP 2 Score: 56.6102 bits (135), Expect = 8.934e-10
Identity = 32/104 (30.77%), Postives = 52/104 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            P+H+A      EI  LL+E+  D  A DK +  P+H A  +G+   ++LL++ +      D +    +H  C+   +E  +LL+E    I   +K E  PL IA
Sbjct:  109 PLHYAASKNQYEIALLLLENGADPNATDKLESTPLHRASAKGNYRLIQLLLKQSASTNIQDSEGNTALHLACDEERVEAAKLLVEHGASIYIENKEEKTPLQIA 212          
BLAST of SMED30016539 vs. Ensembl Cavefish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 79.7221 bits (195), Expect = 2.521e-17
Identity = 39/125 (31.20%), Postives = 71/125 (56.80%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + K  D +  +H A   GH E+VKLL+EH  +  +       P+H+A  +GH++ V++L++ N     + K+   P+H   + G +++ ELL+ER  + NA  K  + PL +A+ + N ++V +L
Sbjct:  436 DAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLL 560          

HSP 2 Score: 70.8626 bits (172), Expect = 3.174e-14
Identity = 34/116 (29.31%), Postives = 65/116 (56.03%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC   H  ++ LL++H+  +EA+ +    P+H+A + GH   VK+L++      A +   + P+H    +G+ E+ E L++    ++A+ K +   L  A +  +KELV++L
Sbjct:  346 PLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLL 461          

HSP 3 Score: 60.4622 bits (145), Expect = 1.149e-10
Identity = 30/116 (25.86%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G A++V LLI    ++   +K    P+HL   +GH     +L++    + A  +    P+H  C  G I++V+ L++++ ++N + +    PL  A +  + ++V +L
Sbjct:  610 PLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLL 725          

HSP 4 Score: 59.3066 bits (142), Expect = 2.679e-10
Identity = 39/145 (26.90%), Postives = 72/145 (49.66%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--ALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R G+V + R       + + K  D   P+H A   GH  I+++L++    I+A  K    PIH+A    H + V+ L+++N +++   LD   P+H     G+  + ++L+++    N R      PL IA K  +  ++++L
Sbjct:  218 ASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLL 362          

HSP 5 Score: 53.5286 bits (127), Expect = 2.284e-8
Identity = 28/116 (24.14%), Postives = 62/116 (53.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A    +  + +LL+    ++    K    P+H+A  +G+   V+LL++    ++A  K    P+H    +G++ I+E+L+++   I A+ K  ++P+ +A + ++ + V  L
Sbjct:  181 PLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQL 296          

HSP 6 Score: 51.6026 bits (122), Expect = 1.026e-7
Identity = 29/116 (25.00%), Postives = 56/116 (48.28%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A      E+   L+++     A   Q   P+HLA  +G ++ V LLI    ++   +K    P+H   + G++ I ++L+++   + A  +    PL +A    N ++V+ L
Sbjct:  577 PLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFL 692          

HSP 7 Score: 50.447 bits (119), Expect = 2.760e-7
Identity = 28/116 (24.14%), Postives = 58/116 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   G+  +V+LL++    I+A  K +  P+H A   GH   +++L++    ++A  K    PIH   +  +++ V  L++   +I+      + PL +A    +  + ++L
Sbjct:  214 PLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVL 329          

HSP 8 Score: 48.9062 bits (115), Expect = 1.050e-6
Identity = 23/91 (25.27%), Postives = 45/91 (49.45%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDIN 476
            P+H     GH  I  +L++    + A  +    P+H+AC+ G+ + VK L++   ++    +    P+H   + G+ +IV LL++     N
Sbjct:  643 PLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKHGAQPN 733          
BLAST of SMED30016539 vs. Ensembl Cavefish
Match: psmd10 (proteasome 26S subunit, non-ATPase 10 [Source:NCBI gene;Acc:103037594])

HSP 1 Score: 75.8702 bits (185), Expect = 1.014e-16
Identity = 40/122 (32.79%), Postives = 62/122 (50.82%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            + D R  +H AC AGHA IV+ L++   +++  D     P+H+A   G  E V+ LI     + ++++    P+H+       EI  LL+E   D NA DK E  PL  A    N  L+++L
Sbjct:   51 NSDHRTALHWACSAGHANIVQFLLDVGAEVDLKDDASWTPLHIAASAGREEIVRSLISKGAQLNSVNQNGCTPLHYAASKNQYEIALLLLENGADPNATDKLESTPLHRASAKGNYRLIQLL 172          

HSP 2 Score: 56.6102 bits (135), Expect = 8.987e-10
Identity = 32/104 (30.77%), Postives = 52/104 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            P+H+A      EI  LL+E+  D  A DK +  P+H A  +G+   ++LL++ +      D +    +H  C+   +E  +LL+E    I   +K E  PL IA
Sbjct:  123 PLHYAASKNQYEIALLLLENGADPNATDKLESTPLHRASAKGNYRLIQLLLKQSASTNIQDSEGNTALHLACDEERVEAAKLLVEHGASIYIENKEEKTPLQIA 226          
BLAST of SMED30016539 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.1889 bits (204), Expect = 3.555e-19
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F GH+E+V  L+    +I A DK+ +R IH A Y GH+E VK+L+ H  D+   DK+   P+H    SG I +V+LL++   DI+  +      L +A  N
Sbjct:  139 RTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYN 247          

HSP 2 Score: 64.6994 bits (156), Expect = 8.510e-13
Identity = 41/156 (26.28%), Postives = 67/156 (42.95%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            KD     P+H AC +     V+ L++H+ D+ A DK  Q P+H+A                                  + GHSE V  L+    ++ A DK+    IHW    G+ E+V++L+    D+  +DK   +PL  A  +    +V++L
Sbjct:   68 KDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLL 223          

HSP 3 Score: 62.3882 bits (150), Expect = 4.383e-12
Identity = 44/149 (29.53%), Postives = 70/149 (46.98%), Query Frame = 3
Query:  120 FINAIRSGSVELFRR--YFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
             I AI +G  +  R   Y KEE +   D + R P+H A + G AEI++LL+     + + D +   P+H AC       V+ L++H+ D+ A DK    P+H    +  ++  E +I      N  D+     L  A  N + E+V  L
Sbjct:   10 LIQAIFNGDPDEVRSLIYKKEEVNAVDD-EKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFL 157          

HSP 4 Score: 61.2326 bits (147), Expect = 1.214e-11
Identity = 35/111 (31.53%), Postives = 60/111 (54.05%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEH---NCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            D R  +H A   GH E ++LL+E+   N  ++A D  +Q P+ LA   GH++   LL+     + A D +    +H    +G+ E V+LL++R  D++ R++    PL +A
Sbjct:  649 DRRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMA 759          

HSP 5 Score: 54.6842 bits (130), Expect = 2.333e-9
Identity = 32/120 (26.67%), Postives = 64/120 (53.33%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDM---EALDKQ-PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A + GH   +++LI    D++  +     P+ LA  +GH++ V++LI+H   +   E  D++  +H    +G++E + LL+E       ++A D  E  P+ +A+ N + +   +L
Sbjct:  586 PLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKEGPDRRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLL 705          

HSP 6 Score: 51.9878 bits (123), Expect = 1.747e-8
Identity = 37/146 (25.34%), Postives = 63/146 (43.15%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIH---------------------------------LACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPLDI 512
            ++KD   RR IH A + GH E+VK+L+ H  D+   DK+   P+H                                 +ACY G    V  LI+   ++   +++   P+H+   S +  + +ELL+    D+N + K   +PL +
Sbjct:  168 DKKD---RRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQSKDGKSPLHM 310          

HSP 7 Score: 49.2914 bits (116), Expect = 1.206e-7
Identity = 43/175 (24.57%), Postives = 67/175 (38.29%), Query Frame = 3
Query:  153 LFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIE----HNCDIEALDKQKQRPIHLACYQGHSEPVKLLIE---------------H-----------------------NCDMEALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            L +R       ER+    R P+H A   GH  I+  L++        I   D+    P+H ACY GH   V+LL+E               H                       NC  +A  + P+H    + ++E ++LL+     +N  D    +PL +A +N     VE+L
Sbjct:  738 LLQRSVDVSLRERRG---RAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCP-DAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVL 908          

HSP 8 Score: 47.7506 bits (112), Expect = 4.850e-7
Identity = 41/186 (22.04%), Postives = 70/186 (37.63%), Query Frame = 3
Query:  126 NAIRSGSVELFRRYFKE----EFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ----------------------------------------PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +A  +G VE  R   +     E  +  D + + P+  A   GH +   LL+     I A D +    +H     GH E V LL++ + D+   +++                                        P+HW C +G+   VELL+E       ++    +PL  A+ N+N+   E+L
Sbjct:  656 SAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDV-FQKQEGNAFSPLHCAVLNDNESCAEML 840          

HSP 9 Score: 45.8246 bits (107), Expect = 1.889e-6
Identity = 19/57 (33.33%), Postives = 31/57 (54.39%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLI 365
            D   R P+H A F  H E ++LL+ HN  +   D   + P+ +AC  G +  V++L+
Sbjct:  853 DAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLV 909          

HSP 10 Score: 45.8246 bits (107), Expect = 2.154e-6
Identity = 36/139 (25.90%), Postives = 56/139 (40.29%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIE-----------------------HNCD-----------IEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIER-KCDINARDKCEMNPLDIA 515
            P+H AC+ GH   V+LL+E                        +C            I   D + + P+H A +  H E ++LL+ HN  +   D   K P+   CE+G    VE+L+   K D+  +D      L +A
Sbjct:  792 PLHWACYNGHDGCVELLLELDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLVNSTKTDLKLQDANRNTALHLA 930          
BLAST of SMED30016539 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007954.1 (pep scaffold:Pmarinus_7.0:GL479092:583:36301:1 gene:ENSPMAG00000007165.1 transcript:ENSPMAT00000007954.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.9518 bits (193), Expect = 8.878e-18
Identity = 41/118 (34.75%), Postives = 67/118 (56.78%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            R  +HHA F G+ E+V LLI    ++ A+DK+++R +H A Y GH+E VK+L+ +  D+   DK+   P+H    SG++ +V LL+  K ++N  +      L +A  N    +V  L
Sbjct:   48 RTALHHAAFNGNLEMVNLLISKGANVNAMDKRERRAVHWAGYMGHTEVVKMLLSNGADVNWKDKKGYTPLHAAAASGHVTVVRLLLSLKAEMNEFNGFGNTALHVACHNGQDAVVSEL 165          

HSP 2 Score: 55.4546 bits (132), Expect = 1.119e-9
Identity = 33/123 (26.83%), Postives = 61/123 (49.59%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D + + P+H A      +    LI    ++   D+  +  +H A + G+ E V LLI    ++ A+DK   + +HW    G+ E+V++L+    D+N +DK    PL  A  + +  +V +L
Sbjct:   10 RDKNWQTPLHVASANRAIKCADALIPLLSNVNVSDRAGRTALHHAAFNGNLEMVNLLISKGANVNAMDKRERRAVHWAGYMGHTEVVKMLLSNGADVNWKDKKGYTPLHAAAASGHVTVVRLL 132          

HSP 3 Score: 43.8986 bits (102), Expect = 7.945e-6
Identity = 30/120 (25.00%), Postives = 51/120 (42.50%), Query Frame = 3
Query:  201 DLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            D + P+H     G     + LI++  +I+  DK+   P+H+A   GH   +  LI +  D          P+H    SG+ +    L+    D++A D+     L  A    N E + +L
Sbjct:  212 DGKSPLHMTAIHGRFTRSQTLIQNGGEIDCGDKEGNTPLHIAARYGHELLINTLISNGADAARRGSHGMFPLHLAALSGFSDCCRKLLTPGFDVDAVDEFGRTCLHAAAAGGNAECINLL 331          
BLAST of SMED30016539 vs. Ensembl Sea Lamprey
Match: ankrd1a (ankyrin repeat domain 1a (cardiac muscle) [Source:ZFIN;Acc:ZDB-GENE-100405-1])

HSP 1 Score: 72.4034 bits (176), Expect = 1.746e-16
Identity = 41/133 (30.83%), Postives = 62/133 (46.62%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRYFKEEFSERK-DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPL 506
            F+ A   G VE+   Y ++       D   R  +H +  AGH E+V+ LI+    +E  DK K   +H A   GH   ++ L++    ++A D     P+H     G   + E LIER   +NA+DK    PL
Sbjct:    6 FLKAAARGKVEVVEEYLQQGGDPDSCDATHRTALHQSSQAGHLEVVQKLIDKGAQLENKDKLKSTAMHCASRGGHVAVLETLLDSGASIKATDSILSSPLHVAARVGQYNVAEFLIERGASVNAQDKEGDTPL 138          
BLAST of SMED30016539 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 68.9366 bits (167), Expect = 2.750e-14
Identity = 40/145 (27.59%), Postives = 73/145 (50.34%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A R+G  E+ R   +     + K  + + P+H A   G  EIV+LL++H    +A       P+H+A  +GH +   +L+EH   +    K+   P+H   + G +E+  LL++R    +A  K  + PL +A   +N+++  +L
Sbjct:   88 AARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLL 232          

HSP 2 Score: 57.7658 bits (138), Expect = 1.746e-10
Identity = 31/116 (26.72%), Postives = 54/116 (46.55%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A      EI   L+E+  +  A+ +Q   P+HL   +GH++   LL+    D+    K    P+H   +   + + E+L++    +NA  K    PL +A    N ++V  L
Sbjct:  249 PLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFL 364          

HSP 3 Score: 57.3806 bits (137), Expect = 2.132e-10
Identity = 31/115 (26.96%), Postives = 57/115 (49.57%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A  AG  E+V+ L+ +   ++A  +++Q P+H+A   G  E V+LL++H    +A       P+H     G+ ++  +L+E    +    K    PL +A K    E+  +L
Sbjct:   85 LHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLL 199          

HSP 4 Score: 57.3806 bits (137), Expect = 2.152e-10
Identity = 30/116 (25.86%), Postives = 57/116 (49.14%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A    + ++  LL++      A  K    P+H+A  +   E    L+E+  +  A+ +Q   P+H   + G+ ++  LL+ R  D+N   K  ++PL +A + +   + EIL
Sbjct:  216 PLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEIL 331          

HSP 5 Score: 54.6842 bits (130), Expect = 1.940e-9
Identity = 29/116 (25.00%), Postives = 60/116 (51.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC      +++LL+++   IEA+ +    P+H++ + G+   V +L++      A +   +  +H    +G  E+V  L+     ++A+ + E  PL IA +    E+V++L
Sbjct:   18 PLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLL 133          

HSP 6 Score: 50.447 bits (119), Expect = 6.452e-8
Identity = 30/112 (26.79%), Postives = 52/112 (46.43%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKEL 539
            P+H A       + ++L++H   + A  K    P+ +AC+ G+ + V  L++H  D+ +  K    P+H   + G+  IV LL+          KC   P D+ + N N  L
Sbjct:  315 PLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL----------KCGARPNDVTV-NGNTAL 415          
BLAST of SMED30016539 vs. Ensembl Sea Lamprey
Match: fem1c (fem-1 homolog c [Source:NCBI gene;Acc:378965])

HSP 1 Score: 68.5514 bits (166), Expect = 3.264e-14
Identity = 40/116 (34.48%), Postives = 61/116 (52.59%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDM---EALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+  ACF GH +IVK LIEH  D+E  ++     + +ACY+GH E  K L+E   D+          +H   ESG +EI+++L++    +  +D   M PL  A    +  +VE L
Sbjct:  119 PLRAACFDGHLDIVKYLIEHQADLEVANRHGHTCLMIACYKGHKEIAKYLLERGADVNRKSVKGNTALHDCAESGSLEIMKMLLKYSAHME-KDGYGMTPLMAASVTGHTNIVEYL 233          

HSP 2 Score: 60.077 bits (144), Expect = 2.796e-11
Identity = 31/93 (33.33%), Postives = 47/93 (50.54%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQP---IHWECESGYIEIVELLIERKCDINAR 482
            P+  A  AGH  +VK L+ H   +         P+  AC+ GH + VK LIEH  D+E  ++     +   C  G+ EI + L+ER  D+N +
Sbjct:   86 PLWAASAAGHLAVVKSLLAHGASVNNTTLTNSTPLRAACFDGHLDIVKYLIEHQADLEVANRHGHTCLMIACYKGHKEIAKYLLERGADVNRK 178          
BLAST of SMED30016539 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 44.2838 bits (103), Expect = 2.305e-6
Identity = 35/129 (27.13%), Postives = 57/129 (44.19%), Query Frame = 3
Query:  189 RKDVDLRRPIHHACFAGHAEIVKLLIE--HNCDIEAL-DKQKQRPIHLACYQGHSEPVKLLIEHNC--DMEALDKQPI---HWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +KD D R P+H +      EI   L+    N +++   D     P H+AC  G+ E VK L +     D+  +  Q +   H      + E+ + LIE    +  +DK    PL  A    + +L+E+L
Sbjct:   31 QKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELL 159          
BLAST of SMED30016539 vs. Ensembl Nematostella
Match: EDO30889 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZG9])

HSP 1 Score: 64.6994 bits (156), Expect = 6.509e-14
Identity = 42/120 (35.00%), Postives = 65/120 (54.17%), Query Frame = 3
Query:  117 LFINAIRSGSVELFRRYFKEEFSERKDVD--LRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIER 461
            +FI +   G+ ++  R      ++ K VD     P+H A  AG  +I KLLI+   D+ AL+K+ Q P+HL+   G SE  +LL++   D   LD Q   P+    +SG I+++E  IER
Sbjct:    1 MFILSWTFGTADVLVR----RGADVKAVDHHGNTPLHCAAAAGRDDIAKLLIDKWSDVNALNKEDQSPLHLSARVGSSEVTQLLLDKGADPRLLDAQARTPMEMAIDSGRIDVIE-AIER 115          

HSP 2 Score: 57.3806 bits (137), Expect = 4.090e-11
Identity = 28/101 (27.72%), Postives = 52/101 (51.49%), Query Frame = 3
Query:  258 LLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEAL---DKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +L+    D++A+D     P+H A   G  +  KLLI+   D+ AL   D+ P+H     G  E+ +LL+++  D    D     P+++AI +   +++E +
Sbjct:   13 VLVRRGADVKAVDHHGNTPLHCAAAAGRDDIAKLLIDKWSDVNALNKEDQSPLHLSARVGSSEVTQLLLDKGADPRLLDAQARTPMEMAIDSGRIDVIEAI 113          
BLAST of SMED30016539 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57])

HSP 1 Score: 67.0106 bits (162), Expect = 1.837e-13
Identity = 37/112 (33.04%), Postives = 61/112 (54.46%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKEL 539
            P+H A   GHA +VK L+     I+A +     P+  A  +G  E   +L++ +  ++  DK    P+H     G++E+V+LL+ RK +I   D    N LDIAI++ +KE+
Sbjct:  320 PLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEV 431          

HSP 2 Score: 50.8322 bits (120), Expect = 7.225e-8
Identity = 27/102 (26.47%), Postives = 51/102 (50.00%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLI-------------EHNCDMEALDKQPIHWECESGYIEIVELLIERKCDINA 479
            P+H AC  G   + K L+E   D+ +LD+++  P+H AC +G+    KLL              +   D +  ++  +H   E G+ ++ +L +E+  ++NA
Sbjct:    7 PLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNA 108          
BLAST of SMED30016539 vs. Ensembl Nematostella
Match: EDO41101 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5D3])

HSP 1 Score: 53.9138 bits (128), Expect = 4.421e-10
Identity = 34/100 (34.00%), Postives = 49/100 (49.00%), Query Frame = 3
Query:  279 DIEALDKQKQRPIHLACYQGHSEPVKLL-----IEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELV-EIL 551
            D+   D  +  P+H A   GH   V+ L     I  +  +EA D     P+      G   +V++LI+ K D+ AR+   M  LDIAI+N N  +V EIL
Sbjct:    1 DVSTKDSSRWTPLHHAAGAGHVRTVETLMRATNIGFSYSLEAEDWRGNTPLMTAAGRGRDRVVQVLIQHKADVTARNAKGMTALDIAIENANATVVMEIL 100          
BLAST of SMED30016539 vs. Ensembl Nematostella
Match: EDO41104 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5D3])

HSP 1 Score: 53.9138 bits (128), Expect = 4.421e-10
Identity = 34/100 (34.00%), Postives = 49/100 (49.00%), Query Frame = 3
Query:  279 DIEALDKQKQRPIHLACYQGHSEPVKLL-----IEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELV-EIL 551
            D+   D  +  P+H A   GH   V+ L     I  +  +EA D     P+      G   +V++LI+ K D+ AR+   M  LDIAI+N N  +V EIL
Sbjct:    1 DVSTKDSSRWTPLHHAAGAGHVRTVETLMRATNIGFSYSLEAEDWRGNTPLMTAAGRGRDRVVQVLIQHKADVTARNAKGMTALDIAIENANATVVMEIL 100          
BLAST of SMED30016539 vs. Ensembl Nematostella
Match: EDO28023 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T7M5])

HSP 1 Score: 53.5286 bits (127), Expect = 5.600e-10
Identity = 31/96 (32.29%), Postives = 46/96 (47.92%), Query Frame = 3
Query:  279 DIEALDKQKQRPIHLACYQGHSEPVKLL-----IEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELV 542
            D+   D  +  P+H A   GH   V+ L     I  +  +EA D     P+      G   +V++LI+ K D+ AR+   M  LDIAI+N N  +V
Sbjct:    1 DVSTKDSSRWTPLHHAAGAGHVRTVETLMRATNIGFSYSLEAEDWRGNTPLMTAAGRGRDRVVQVLIQHKADVTARNAKGMTALDIAIENANATVV 96          
BLAST of SMED30016539 vs. Ensembl Medaka
Match: ankrd28b (ankyrin repeat domain 28 [Source:NCBI gene;Acc:101159131])

HSP 1 Score: 86.6557 bits (213), Expect = 9.539e-20
Identity = 44/109 (40.37%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V+LL+    +I A DK+ +R IH A Y GH E VKLL  H  ++   DK+   P+H    SG I +V+ L++   DIN  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYN 249          

HSP 2 Score: 64.3142 bits (155), Expect = 4.663e-12
Identity = 39/131 (29.77%), Postives = 66/131 (50.38%), Query Frame = 3
Query:  168 FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            FK+E    +D + R P+H A + G AEI++LLI     + A D +   P+H A      E V++L++H+ D+ A DK    P+H    +  +   E L+    ++N  D+     L  A  + + E+V +L
Sbjct:   29 FKKEDVNAQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLL 159          

HSP 3 Score: 63.929 bits (154), Expect = 6.149e-12
Identity = 42/156 (26.92%), Postives = 67/156 (42.95%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E V+LL+    ++ A DK+    IHW    G+IE+V+LL     ++  +DK    PL  A  +    +V+ L
Sbjct:   70 KDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFL 225          

HSP 4 Score: 63.1586 bits (152), Expect = 1.409e-11
Identity = 40/116 (34.48%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+  A F    + V+ LI    D+ A D +K+ P+H A Y G +E ++LLI     + A D +   P+H    S   E V++L++   D+NARDK    PL IA  N+     E L
Sbjct:   11 PLLKAIFNVDPDEVRSLIFKKEDVNAQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEAL 126          

HSP 5 Score: 61.6178 bits (148), Expect = 4.677e-11
Identity = 37/135 (27.41%), Postives = 62/135 (45.93%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDM---EALDKQPIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKN 524
            G+ E+            KD   R P+H A F  H E ++LL+ HN  +  +D   + P+ +A   G +  V+LL+     D+   +A+    +H  C  G+     L++E+  D   INA +     PL +A +N
Sbjct:  802 GAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELLVSSAKADLTIQDAVKNTALHLACSKGHETSALLILEKISDRNLINATNAALQTPLHVAARN 936          

HSP 6 Score: 55.4546 bits (132), Expect = 5.500e-9
Identity = 36/129 (27.91%), Postives = 61/129 (47.29%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEAL-------DKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E KD   R  +H     GH E ++ L++HN      D + + P+HLA   GH   +  L+     +E L          P+HW C +G+   VE+L+E++   +  +    +PL  A+  +N+   E+L
Sbjct:  680 EAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLEQEV-FHKAEGNPFSPLHCAVIRDNEGAAEML 807          

HSP 7 Score: 54.6842 bits (130), Expect = 1.018e-8
Identity = 33/118 (27.97%), Postives = 62/118 (52.54%), Query Frame = 3
Query:  195 DVDLRRPI---HHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAI 518
            D D+R P+   H A + GH + +++L++   D++  + Q + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   + +  ++ +D     PL +A+
Sbjct:  544 DSDVRAPVSPLHLAAYHGHHQAMEVLVQSLLDLDVRNSQGRTPLDLAAFKGHVECVDVLINQGASILVKDFTLKRTPIHAAATNGHSECLRLLIGNADLQSAVDIQDGNGQTPLMLAV 661          

HSP 8 Score: 50.8322 bits (120), Expect = 2.214e-7
Identity = 32/111 (28.83%), Postives = 54/111 (48.65%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPLDI 512
            KD     P+H A  +G   +VK L++   DI   +     P+H+ACY G    V  LIE    +  ++++   P+H+   S +  + +ELL+    D+N + K    PL +
Sbjct:  202 KDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYNGQDVVVNELIECGAHVNQVNEKGFAPLHFTAASRHGALCLELLVCNGADVNIKSKDGKTPLHM 312          

HSP 9 Score: 48.9062 bits (115), Expect = 9.894e-7
Identity = 35/139 (25.18%), Postives = 58/139 (41.73%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIE----HNCD------------------------------IEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIER-KCDINARDKCEMNPLDIA 515
            P+H AC+ GH   V++L+E    H  +                              + A D + + P+H A +  H E ++LL+ HN  +  +D   K P+    E+G    VELL+   K D+  +D  +   L +A
Sbjct:  759 PLHWACYNGHDTCVEVLLEQEVFHKAEGNPFSPLHCAVIRDNEGAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELLVSSAKADLTIQDAVKNTALHLA 897          

HSP 10 Score: 47.7506 bits (112), Expect = 2.021e-6
Identity = 36/161 (22.36%), Postives = 63/161 (39.13%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEH--------------NCDM-----------------------EALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   R P+H A   GH  ++  L+     +E L    D     P+H ACY GH   V++L+E               +C +                       +  ++ P+H    + ++E ++LL+     +N  D    +PL +A +N     VE+L
Sbjct:  715 RDCRGRTPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLEQEVFHKAEGNPFSPLHCAVIRDNEGAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELL 875          

HSP 11 Score: 46.2098 bits (108), Expect = 7.659e-6
Identity = 32/127 (25.20%), Postives = 55/127 (43.31%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINA----RDKCEMNPLDIAIKNENKELVEIL 551
            +D + + P+  A  +GH + V  L+     +EA DK  +  +H     GH E ++ L++HN      D   + P+H     G+I ++  L+     +       D     PL  A  N +   VE+L
Sbjct:  649 QDGNGQTPLMLAVLSGHTDCVYSLLNKGASVEAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVL 775          
BLAST of SMED30016539 vs. Ensembl Medaka
Match: ankrd28b (ankyrin repeat domain 28 [Source:NCBI gene;Acc:101159131])

HSP 1 Score: 86.6557 bits (213), Expect = 1.048e-19
Identity = 44/109 (40.37%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V+LL+    +I A DK+ +R IH A Y GH E VKLL  H  ++   DK+   P+H    SG I +V+ L++   DIN  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYN 249          

HSP 2 Score: 64.3142 bits (155), Expect = 4.928e-12
Identity = 39/131 (29.77%), Postives = 66/131 (50.38%), Query Frame = 3
Query:  168 FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            FK+E    +D + R P+H A + G AEI++LLI     + A D +   P+H A      E V++L++H+ D+ A DK    P+H    +  +   E L+    ++N  D+     L  A  + + E+V +L
Sbjct:   29 FKKEDVNAQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLL 159          

HSP 3 Score: 63.929 bits (154), Expect = 6.498e-12
Identity = 42/156 (26.92%), Postives = 67/156 (42.95%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E V+LL+    ++ A DK+    IHW    G+IE+V+LL     ++  +DK    PL  A  +    +V+ L
Sbjct:   70 KDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFL 225          

HSP 4 Score: 62.7734 bits (151), Expect = 1.532e-11
Identity = 40/116 (34.48%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+  A F    + V+ LI    D+ A D +K+ P+H A Y G +E ++LLI     + A D +   P+H    S   E V++L++   D+NARDK    PL IA  N+     E L
Sbjct:   11 PLLKAIFNVDPDEVRSLIFKKEDVNAQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEAL 126          

HSP 5 Score: 61.2326 bits (147), Expect = 5.037e-11
Identity = 37/135 (27.41%), Postives = 62/135 (45.93%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDM---EALDKQPIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKN 524
            G+ E+            KD   R P+H A F  H E ++LL+ HN  +  +D   + P+ +A   G +  V+LL+     D+   +A+    +H  C  G+     L++E+  D   INA +     PL +A +N
Sbjct:  784 GAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELLVSSAKADLTIQDAVKNTALHLACSKGHETSALLILEKISDRNLINATNAALQTPLHVAARN 918          

HSP 6 Score: 50.8322 bits (120), Expect = 2.231e-7
Identity = 32/111 (28.83%), Postives = 54/111 (48.65%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPLDI 512
            KD     P+H A  +G   +VK L++   DI   +     P+H+ACY G    V  LIE    +  ++++   P+H+   S +  + +ELL+    D+N + K    PL +
Sbjct:  202 KDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYNGQDVVVNELIECGAHVNQVNEKGFAPLHFTAASRHGALCLELLVCNGADVNIKSKDGKTPLHM 312          

HSP 7 Score: 46.2098 bits (108), Expect = 8.087e-6
Identity = 24/79 (30.38%), Postives = 43/79 (54.43%), Query Frame = 3
Query:  195 DVDLRRPI---HHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIH 410
            D D+R P+   H A + GH + +++L++   D++  + Q + P+ LA ++GH E V +LI     +   D    + PIH
Sbjct:  540 DSDVRAPVSPLHLAAYHGHHQAMEVLVQSLLDLDVRNSQGRTPLDLAAFKGHVECVDVLINQGASILVKDFTLKRTPIH 618          
BLAST of SMED30016539 vs. Ensembl Medaka
Match: ankrd28b (ankyrin repeat domain 28 [Source:NCBI gene;Acc:101159131])

HSP 1 Score: 86.2705 bits (212), Expect = 1.388e-19
Identity = 44/109 (40.37%), Postives = 62/109 (56.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V+LL+    +I A DK+ +R IH A Y GH E VKLL  H  ++   DK+   P+H    SG I +V+ L++   DIN  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYN 249          

HSP 2 Score: 63.929 bits (154), Expect = 6.574e-12
Identity = 39/131 (29.77%), Postives = 66/131 (50.38%), Query Frame = 3
Query:  168 FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            FK+E    +D + R P+H A + G AEI++LLI     + A D +   P+H A      E V++L++H+ D+ A DK    P+H    +  +   E L+    ++N  D+     L  A  + + E+V +L
Sbjct:   29 FKKEDVNAQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLL 159          

HSP 3 Score: 63.5438 bits (153), Expect = 8.423e-12
Identity = 42/156 (26.92%), Postives = 67/156 (42.95%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLAC---------------------------------YQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            KD     P+H A  +   E V++L++H+ D+ A DK  Q P+H+A                                  + GH E V+LL+    ++ A DK+    IHW    G+IE+V+LL     ++  +DK    PL  A  +    +V+ L
Sbjct:   70 KDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFL 225          

HSP 4 Score: 62.7734 bits (151), Expect = 1.930e-11
Identity = 40/116 (34.48%), Postives = 59/116 (50.86%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+  A F    + V+ LI    D+ A D +K+ P+H A Y G +E ++LLI     + A D +   P+H    S   E V++L++   D+NARDK    PL IA  N+     E L
Sbjct:   11 PLLKAIFNVDPDEVRSLIFKKEDVNAQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNKWLTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVRCAEAL 126          

HSP 5 Score: 61.2326 bits (147), Expect = 6.106e-11
Identity = 37/135 (27.41%), Postives = 62/135 (45.93%), Query Frame = 3
Query:  141 GSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH-NCDM---EALDKQPIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKN 524
            G+ E+            KD   R P+H A F  H E ++LL+ HN  +  +D   + P+ +A   G +  V+LL+     D+   +A+    +H  C  G+     L++E+  D   INA +     PL +A +N
Sbjct:  802 GAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELLVSSAKADLTIQDAVKNTALHLACSKGHETSALLILEKISDRNLINATNAALQTPLHVAARN 936          

HSP 6 Score: 55.0694 bits (131), Expect = 7.448e-9
Identity = 36/129 (27.91%), Postives = 61/129 (47.29%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEAL-------DKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E KD   R  +H     GH E ++ L++HN      D + + P+HLA   GH   +  L+     +E L          P+HW C +G+   VE+L+E++   +  +    +PL  A+  +N+   E+L
Sbjct:  680 EAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLEQEV-FHKAEGNPFSPLHCAVIRDNEGAAEML 807          

HSP 7 Score: 54.299 bits (129), Expect = 1.404e-8
Identity = 33/118 (27.97%), Postives = 62/118 (52.54%), Query Frame = 3
Query:  195 DVDLRRPI---HHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLI---ERKCDINARDKCEMNPLDIAI 518
            D D+R P+   H A + GH + +++L++   D++  + Q + P+ LA ++GH E V +LI     +   D    + PIH    +G+ E + LLI   + +  ++ +D     PL +A+
Sbjct:  544 DSDVRAPVSPLHLAAYHGHHQAMEVLVQSLLDLDVRNSQGRTPLDLAAFKGHVECVDVLINQGASILVKDFTLKRTPIHAAATNGHSECLRLLIGNADLQSAVDIQDGNGQTPLMLAV 661          

HSP 8 Score: 50.447 bits (119), Expect = 2.649e-7
Identity = 32/109 (29.36%), Postives = 53/109 (48.62%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEI-VELLIERKCDINARDKCEMNPL 506
            KD     P+H A  +G   +VK L++   DI   +     P+H+ACY G    V  LIE    +  ++++   P+H+   S +  + +ELL+    D+N + K    PL
Sbjct:  202 KDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYNGQDVVVNELIECGAHVNQVNEKGFAPLHFTAASRHGALCLELLVCNGADVNIKSKDGKTPL 310          

HSP 9 Score: 48.521 bits (114), Expect = 1.350e-6
Identity = 35/139 (25.18%), Postives = 58/139 (41.73%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIE----HNCD------------------------------IEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIER-KCDINARDKCEMNPLDIA 515
            P+H AC+ GH   V++L+E    H  +                              + A D + + P+H A +  H E ++LL+ HN  +  +D   K P+    E+G    VELL+   K D+  +D  +   L +A
Sbjct:  759 PLHWACYNGHDTCVEVLLEQEVFHKAEGNPFSPLHCAVIRDNEGAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELLVSSAKADLTIQDAVKNTALHLA 897          

HSP 10 Score: 47.3654 bits (111), Expect = 2.730e-6
Identity = 36/161 (22.36%), Postives = 63/161 (39.13%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEAL----DKQKQRPIHLACYQGHSEPVKLLIEH--------------NCDM-----------------------EALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +D   R P+H A   GH  ++  L+     +E L    D     P+H ACY GH   V++L+E               +C +                       +  ++ P+H    + ++E ++LL+     +N  D    +PL +A +N     VE+L
Sbjct:  715 RDCRGRTPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLEQEVFHKAEGNPFSPLHCAVIRDNEGAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELL 875          

HSP 11 Score: 45.8246 bits (107), Expect = 9.772e-6
Identity = 32/127 (25.20%), Postives = 55/127 (43.31%), Query Frame = 3
Query:  192 KDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINA----RDKCEMNPLDIAIKNENKELVEIL 551
            +D + + P+  A  +GH + V  L+     +EA DK  +  +H     GH E ++ L++HN      D   + P+H     G+I ++  L+     +       D     PL  A  N +   VE+L
Sbjct:  649 QDGNGQTPLMLAVLSGHTDCVYSLLNKGASVEAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVL 775          
BLAST of SMED30016539 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 80.1073 bits (196), Expect = 1.726e-17
Identity = 45/145 (31.03%), Postives = 77/145 (53.10%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A RSG  E+     +     + K  D + P+H A   GH E+VKLL+EH  +  +       P+H+A  +GH + V+LL++       + K+   P+H   + G +++ ELL+ER  + NA  K  + PL +A+ + N ++V +L
Sbjct:  382 ASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLL 526          

HSP 2 Score: 76.2554 bits (186), Expect = 3.508e-16
Identity = 37/116 (31.90%), Postives = 66/116 (56.90%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H AC   H  ++ LL++H+  IEA+ +    P+H+A + GH   VK+L++      A +   + P+H    SG+ E+ E L++    ++A+ K +  PL  A +  +KELV++L
Sbjct:  312 PLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLL 427          

HSP 3 Score: 58.5362 bits (140), Expect = 5.329e-10
Identity = 33/116 (28.45%), Postives = 63/116 (54.31%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDME--ALDK-QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            P+H A   GH  I+++L+++   I+A  K    PIH+A    H + VK L+++N +++   LD   P+H     G+  + ++L+++    N+R      PL IA K  +  ++++L
Sbjct:  213 PLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLL 328          

HSP 4 Score: 54.299 bits (129), Expect = 1.526e-8
Identity = 26/97 (26.80%), Postives = 51/97 (52.58%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCE 494
            P+H A   G  +IV LLI    ++   +K    P+HL   +GH     +L++    + A  +    P+H  C  G I++V+ L++++ ++N++ + E
Sbjct:  576 PLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNSKTRVE 672          

HSP 5 Score: 50.0618 bits (118), Expect = 4.310e-7
Identity = 29/115 (25.22%), Postives = 54/115 (46.96%), Query Frame = 3
Query:  216 IHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +H A      E+   L++H     A   Q   P+HLA  +G  + V LLI    ++   +K    P+H   + G++ I ++L+++   + A  +    PL +A    N ++V+ L
Sbjct:  544 LHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFL 658          
BLAST of SMED30016539 vs. Ensembl Medaka
Match: ENSORLT00000028696.1 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A [Source:NCBI gene;Acc:101168382])

HSP 1 Score: 78.5666 bits (192), Expect = 6.860e-17
Identity = 41/109 (37.61%), Postives = 59/109 (54.13%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKN 524
            R  +HHA F+GH E+V+LL+    +I A DK+ +R IH A Y GH E VKLL+E   +++  DK+   P+H    SG    V  L+     +N  +     PL +A  N
Sbjct:  141 RTALHHAAFSGHTEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHLEVVKLLVESGAEVDCKDKKGYSPLHAAASSGMSSTVHYLLGLGVHVNEANSYGNTPLHLACYN 249          

HSP 2 Score: 65.855 bits (159), Expect = 1.362e-12
Identity = 47/185 (25.41%), Postives = 76/185 (41.08%), Query Frame = 3
Query:  168 FKEEFSERKDVDLRRPIHHACFAGHAEIVKLLI---------------------------------EHNCDIEALDKQKQRPIHL---------------------------------ACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            FK+E    +D + R P+H A + G AEI++LLI                                 +H+ D+ A DK  Q P+H+                                 A + GH+E V+LL+    ++ A DK+    IHW    G++E+V+LL+E   +++ +DK   +PL  A
Sbjct:   29 FKKEDVNIQDSEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNKWLTPLHRAVASCCEDAVAVLLKHSADVNARDKNWQTPLHVAASNKAVRCAEALVPQLSNVNVSDRAGRTALHHAAFSGHTEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHLEVVKLLVESGAEVDCKDKKGYSPLHAA 213          

HSP 3 Score: 56.225 bits (134), Expect = 2.779e-9
Identity = 36/112 (32.14%), Postives = 56/112 (50.00%), Query Frame = 3
Query:  225 ACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A F+   + V+ LI    D+   D +K+ P+H A Y G +E ++LLI     + A D +   P+H    S   + V +L++   D+NARDK    PL +A  N+     E L
Sbjct:   15 AIFSVDPDEVRSLIFKKEDVNIQDSEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNKWLTPLHRAVASCCEDAVAVLLKHSADVNARDKNWQTPLHVAASNKAVRCAEAL 126          

HSP 4 Score: 53.9138 bits (128), Expect = 1.728e-8
Identity = 39/149 (26.17%), Postives = 68/149 (45.64%), Query Frame = 3
Query:  129 AIRSGSVELFRRYFKEEFS-ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIE-------HNCDMEALDKQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            A+ SG +E          S E +D   R  +H     G  + V+ L++    +   D + + P+HLA   GH   +  L++       H    +     P+HW C +GY   VELL+E++   N +     +PL  A+ ++N+ + E+L
Sbjct:  651 AVLSGHIECVYSLLSHGASVEFQDCWGRTALHRGAVTGQEDCVEALLQRQAGVCVKDTRGRTPLHLASACGHVGVLGALLQTAGSSLTHTHLTDNQGYTPLHWACYNGYDACVELLLEQEMVKNIKGN-SFSPLHCAVMSDNEGVAEML 798          

HSP 5 Score: 51.2174 bits (121), Expect = 1.684e-7
Identity = 32/117 (27.35%), Postives = 57/117 (48.72%), Query Frame = 3
Query:  213 PIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD----KQPIHWECESGYIEIVELLIE---RKCDINARDKCEMNPLDIAIKNENKELV 542
            P+H A F GH   +++L+    D++    +   P+ L+C +GH E V LL+ H       D    K  +H    +G+ E + LL+    +  +I+ +D     PL +A+ + + E V
Sbjct:  544 PLHLAAFHGHCGALEVLLSSLLDVDVRSPEGWTPLSLSCSRGHHECVSLLLHHGASPMIHDYMQKKTALHTAAMNGHAECLRLLLSNDNQHMNIDTQDSSGQTPLMLAVLSGHIECV 660          

HSP 6 Score: 50.0618 bits (118), Expect = 3.288e-7
Identity = 37/129 (28.68%), Postives = 59/129 (45.74%), Query Frame = 3
Query:  195 DVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQR-PIHLACYQGHSEPVKLLIEH-NCDMEALDKQ---PIHWECESGYIEIVELLIERKCD---INARDKCEMNPLDIAIKNENKELV-EILG 554
            D   R P+H A F+ H E V LL+ H   +   D Q  R P+ +A   G +  V++L+     D+   D +    +H  C  G+     L++E+  D   +N  +     PL IA +     +V E+LG
Sbjct:  811 DAKGRIPLHAAAFSDHVECVSLLLSHGAQVNVGDVQMHRTPLMMAALNGQTNTVEVLVSSGKADLSLQDTERNTALHLACSKGHETSALLILEKVSDRNLVNCTNAALQTPLHIAARRGLTVVVQELLG 939          
BLAST of SMED30016539 vs. Planmine SMEST
Match: SMESG000017277.1 (SMESG000017277.1)

HSP 1 Score: 213.001 bits (541), Expect = 1.477e-64
Identity = 109/154 (70.78%), Postives = 121/154 (78.57%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            MDEEFKLFINAI+SGS ELFRRYFKEEF ERKD DLRRPIHHAC  GHAEIVKLLIEH CDIEALDK   RPIHLACYQGHSE VKLLIE+ CD+EALDKQ   PIH  C+ G+ E V+LLIE KCDI A DK    P+ ++    + E V++L
Sbjct:    1 MDEEFKLFINAIKSGSAELFRRYFKEEFLERKDSDLRRPIHHACITGHAEIVKLLIEHKCDIEALDKDNWRPIHLACYQGHSEAVKLLIENKCDIEALDKQKQRPIHLACDQGHSEAVKLLIEHKCDIEALDKWNQRPIHLSCIQGHSEAVKLL 154          

HSP 2 Score: 147.132 bits (370), Expect = 6.660e-41
Identity = 77/118 (65.25%), Postives = 87/118 (73.73%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC  GH+E VKLLIEH CDIEALDKQKQRPIHLACYQGHSE VKLLIEH CD+EALDKQ   PIH  C  G+ E V+LLIE KCDI   D+    P+ +A    + E V++L
Sbjct:  235 RRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIENKCDIEVLDEWNRRPIHLACDQGHSEAVKLL 352          

HSP 3 Score: 144.05 bits (362), Expect = 6.990e-40
Identity = 75/125 (60.00%), Postives = 90/125 (72.00%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E  D   +RPIH AC+ GH+E VKLLIEH CDIEALDKQKQRPIHLACYQGHSE VKLLIE+ CD+E LD   ++PIH  C+ G+ E V+LLIE KCDI A DK    P+ +A    +   V++L
Sbjct:  261 EALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIENKCDIEVLDEWNRRPIHLACDQGHSEAVKLLIENKCDIEALDKWNQRPIHLACDQGHSGAVKLL 385          

HSP 4 Score: 142.51 bits (358), Expect = 2.517e-39
Identity = 80/143 (55.94%), Postives = 98/143 (68.53%), Query Frame = 3
Query:  138 SGSVELF--RRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            SG+V+L    +   E   E+K    RRPIHHAC  GH+E VKLLIE+ CDIEALDK  QRPIHLACYQGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A D     P+ +A    + E V++L
Sbjct:  379 SGAVKLLIENKCDIEALDEQK----RRPIHHACIQGHSEAVKLLIENKCDIEALDKWNQRPIHLACYQGHSEAVKLLIENKCDIEALDERNQRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLL 517          

HSP 5 Score: 137.887 bits (346), Expect = 9.454e-38
Identity = 70/117 (59.83%), Postives = 88/117 (75.21%), Query Frame = 3
Query:  210 RPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RPIH +C  GH+E VKLLIEHNCDIEALD + +RPIH+ACYQGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A DK +  P+ +A    + E V++L
Sbjct:  170 RPIHLSCIQGHSEAVKLLIEHNCDIEALDWRNRRPIHIACYQGHSEAVKLLIENKCDIEALDWWNRRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLL 286          

HSP 6 Score: 137.502 bits (345), Expect = 1.314e-37
Identity = 73/118 (61.86%), Postives = 86/118 (72.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC+ GH+E VKLLIE+ CDIEALD   +RPIHLAC QGHSE VKLLIEH CD+EALDKQ   PIH  C  G+ E V+LLIE KCDI A DK +  P+ +A    + E V++L
Sbjct:  202 RRPIHIACYQGHSEAVKLLIENKCDIEALDWWNRRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLL 319          

HSP 7 Score: 132.109 bits (331), Expect = 8.860e-36
Identity = 72/118 (61.02%), Postives = 84/118 (71.19%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC  GH+E VKLLIE+ CDIEALDK  QRPIHLAC QGHS  VKLLIE+ CD+EALD+Q   PIH  C  G+ E V+LLIE KCDI A DK    P+ +A    + E V++L
Sbjct:  334 RRPIHLACDQGHSEAVKLLIENKCDIEALDKWNQRPIHLACDQGHSGAVKLLIENKCDIEALDEQKRRPIHHACIQGHSEAVKLLIENKCDIEALDKWNQRPIHLACYQGHSEAVKLL 451          

HSP 8 Score: 132.109 bits (331), Expect = 1.162e-35
Identity = 69/118 (58.47%), Postives = 87/118 (73.73%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH AC+ GH+E VKLLIE+ CDIEALD++ QRPIHLAC QGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A D+    P+ +A      E V++L
Sbjct:  433 QRPIHLACYQGHSEAVKLLIENKCDIEALDERNQRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLLIENKCDIEALDERNQRPIHLACYYGRSEAVKLL 550          

HSP 9 Score: 130.954 bits (328), Expect = 2.354e-35
Identity = 73/125 (58.40%), Postives = 85/125 (68.00%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E  D   +RPIH AC  GH+E VKLLIEH CDIEALDK  QRPIHL+C QGHSE VKLLIE+ CD+EALDK   +PIH  C  G+ E V+LLIE  CDI A D     P+ IA    + E V++L
Sbjct:   96 EALDKQKQRPIHLACDQGHSEAVKLLIEHKCDIEALDKWNQRPIHLSCIQGHSEAVKLLIENKCDIEALDKRNWRPIHLSCIQGHSEAVKLLIEHNCDIEALDWRNRRPIHIACYQGHSEAVKLL 220          

HSP 10 Score: 130.568 bits (327), Expect = 3.854e-35
Identity = 68/118 (57.63%), Postives = 89/118 (75.42%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH AC  GH+E VKLLIE+ CDIEALD++ QRPIHLACY G SE VKLLIE+ C +EALDK+   PIH  C  G+ +  +LLIE KC+I+ARD+   +P++ A+K  N E+++IL
Sbjct:  499 QRPIHLACDQGHSEAVKLLIENKCDIEALDERNQRPIHLACYYGRSEAVKLLIENKCYIEALDKRNQRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKIL 616          

HSP 11 Score: 122.094 bits (305), Expect = 3.680e-32
Identity = 66/106 (62.26%), Postives = 77/106 (72.64%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            +RPIH AC  GH+E VKLLIE+ CDIEALD + QRPIHLAC QGHSE VKLLIE+ CD+EALD   ++PIH  C  G  E V+LLIE KC I A DK    P+ +A
Sbjct:  466 QRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLLIENKCDIEALDERNQRPIHLACYYGRSEAVKLLIENKCYIEALDKRNQRPIHLA 571          

HSP 12 Score: 94.3597 bits (233), Expect = 1.521e-22
Identity = 48/87 (55.17%), Postives = 64/87 (73.56%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIE 458
            +RPIH AC+ G +E VKLLIE+ C IEALDK+ QRPIHLACY GHS+  KLLIEH C+++A D+    P+ +  + G  EI+++L E
Sbjct:  532 QRPIHLACYYGRSEAVKLLIENKCYIEALDKRNQRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKILNE 618          

HSP 13 Score: 54.299 bits (129), Expect = 1.030e-8
Identity = 31/80 (38.75%), Postives = 48/80 (60.00%), Query Frame = 3
Query:  135 RSGSVELF--RRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIE 368
            RS +V+L    + + E   +R     +RPIH AC+ GH++  KLLIEH C+I+A D+    P+  A   G++E +K+L E
Sbjct:  543 RSEAVKLLIENKCYIEALDKRN----QRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKILNE 618          
BLAST of SMED30016539 vs. Planmine SMEST
Match: SMESG000017277.1 (SMESG000017277.1)

HSP 1 Score: 213.001 bits (541), Expect = 2.015e-64
Identity = 109/154 (70.78%), Postives = 121/154 (78.57%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            MDEEFKLFINAI+SGS ELFRRYFKEEF ERKD DLRRPIHHAC  GHAEIVKLLIEH CDIEALDK   RPIHLACYQGHSE VKLLIE+ CD+EALDKQ   PIH  C+ G+ E V+LLIE KCDI A DK    P+ ++    + E V++L
Sbjct:    1 MDEEFKLFINAIKSGSAELFRRYFKEEFLERKDSDLRRPIHHACITGHAEIVKLLIEHKCDIEALDKDNWRPIHLACYQGHSEAVKLLIENKCDIEALDKQKQRPIHLACDQGHSEAVKLLIEHKCDIEALDKWNQRPIHLSCIQGHSEAVKLL 154          

HSP 2 Score: 146.747 bits (369), Expect = 7.250e-41
Identity = 77/118 (65.25%), Postives = 87/118 (73.73%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC  GH+E VKLLIEH CDIEALDKQKQRPIHLACYQGHSE VKLLIEH CD+EALDKQ   PIH  C  G+ E V+LLIE KCDI   D+    P+ +A    + E V++L
Sbjct:  235 RRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIENKCDIEVLDEWNRRPIHLACDQGHSEAVKLL 352          

HSP 3 Score: 144.05 bits (362), Expect = 7.500e-40
Identity = 75/125 (60.00%), Postives = 90/125 (72.00%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E  D   +RPIH AC+ GH+E VKLLIEH CDIEALDKQKQRPIHLACYQGHSE VKLLIE+ CD+E LD   ++PIH  C+ G+ E V+LLIE KCDI A DK    P+ +A    +   V++L
Sbjct:  261 EALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIENKCDIEVLDEWNRRPIHLACDQGHSEAVKLLIENKCDIEALDKWNQRPIHLACDQGHSGAVKLL 385          

HSP 4 Score: 142.51 bits (358), Expect = 2.518e-39
Identity = 80/143 (55.94%), Postives = 98/143 (68.53%), Query Frame = 3
Query:  138 SGSVELF--RRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            SG+V+L    +   E   E+K    RRPIHHAC  GH+E VKLLIE+ CDIEALDK  QRPIHLACYQGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A D     P+ +A    + E V++L
Sbjct:  379 SGAVKLLIENKCDIEALDEQK----RRPIHHACIQGHSEAVKLLIENKCDIEALDKWNQRPIHLACYQGHSEAVKLLIENKCDIEALDERNQRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLL 517          

HSP 5 Score: 137.887 bits (346), Expect = 1.007e-37
Identity = 70/117 (59.83%), Postives = 88/117 (75.21%), Query Frame = 3
Query:  210 RPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RPIH +C  GH+E VKLLIEHNCDIEALD + +RPIH+ACYQGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A DK +  P+ +A    + E V++L
Sbjct:  170 RPIHLSCIQGHSEAVKLLIEHNCDIEALDWRNRRPIHIACYQGHSEAVKLLIENKCDIEALDWWNRRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLL 286          

HSP 6 Score: 137.502 bits (345), Expect = 1.386e-37
Identity = 73/118 (61.86%), Postives = 86/118 (72.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC+ GH+E VKLLIE+ CDIEALD   +RPIHLAC QGHSE VKLLIEH CD+EALDKQ   PIH  C  G+ E V+LLIE KCDI A DK +  P+ +A    + E V++L
Sbjct:  202 RRPIHIACYQGHSEAVKLLIENKCDIEALDWWNRRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLL 319          

HSP 7 Score: 132.88 bits (333), Expect = 5.159e-36
Identity = 69/118 (58.47%), Postives = 89/118 (75.42%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH AC+ G +E VKLLIE+ C IEALDK  QRPIHLAC QGHSE VKLLIE+ CD+EALDK   +PIH  C  G+ +  +LLIE KC+I+ARD+   +P++ A+K  N E+++IL
Sbjct:  532 QRPIHLACYYGRSEAVKLLIENKCYIEALDKWNQRPIHLACIQGHSEAVKLLIENKCDIEALDKRNQRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKIL 649          

HSP 8 Score: 132.109 bits (331), Expect = 9.395e-36
Identity = 72/118 (61.02%), Postives = 84/118 (71.19%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC  GH+E VKLLIE+ CDIEALDK  QRPIHLAC QGHS  VKLLIE+ CD+EALD+Q   PIH  C  G+ E V+LLIE KCDI A DK    P+ +A    + E V++L
Sbjct:  334 RRPIHLACDQGHSEAVKLLIENKCDIEALDKWNQRPIHLACDQGHSGAVKLLIENKCDIEALDEQKRRPIHHACIQGHSEAVKLLIENKCDIEALDKWNQRPIHLACYQGHSEAVKLL 451          

HSP 9 Score: 130.954 bits (328), Expect = 2.494e-35
Identity = 73/125 (58.40%), Postives = 85/125 (68.00%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E  D   +RPIH AC  GH+E VKLLIEH CDIEALDK  QRPIHL+C QGHSE VKLLIE+ CD+EALDK   +PIH  C  G+ E V+LLIE  CDI A D     P+ IA    + E V++L
Sbjct:   96 EALDKQKQRPIHLACDQGHSEAVKLLIEHKCDIEALDKWNQRPIHLSCIQGHSEAVKLLIENKCDIEALDKRNWRPIHLSCIQGHSEAVKLLIEHNCDIEALDWRNRRPIHIACYQGHSEAVKLL 220          

HSP 10 Score: 125.561 bits (314), Expect = 2.005e-33
Identity = 69/118 (58.47%), Postives = 83/118 (70.34%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH AC  GH+E VKLLIE+ CDIEALD + QRPIHLAC QGHSE VKLLIE+ CD+EALD   ++PIH  C  G  E V+LLIE KC I A DK    P+ +A    + E V++L
Sbjct:  466 QRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLLIENKCDIEALDERNQRPIHLACYYGRSEAVKLLIENKCYIEALDKWNQRPIHLACIQGHSEAVKLL 583          

HSP 11 Score: 53.9138 bits (128), Expect = 1.216e-8
Identity = 25/54 (46.30%), Postives = 37/54 (68.52%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIE 368
            +RPIH AC+ GH++  KLLIEH C+I+A D+    P+  A   G++E +K+L E
Sbjct:  598 QRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKILNE 651          
BLAST of SMED30016539 vs. Planmine SMEST
Match: SMESG000017277.1 (SMESG000017277.1)

HSP 1 Score: 213.001 bits (541), Expect = 2.211e-64
Identity = 109/154 (70.78%), Postives = 121/154 (78.57%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            MDEEFKLFINAI+SGS ELFRRYFKEEF ERKD DLRRPIHHAC  GHAEIVKLLIEH CDIEALDK   RPIHLACYQGHSE VKLLIE+ CD+EALDKQ   PIH  C+ G+ E V+LLIE KCDI A DK    P+ ++    + E V++L
Sbjct:    1 MDEEFKLFINAIKSGSAELFRRYFKEEFLERKDSDLRRPIHHACITGHAEIVKLLIEHKCDIEALDKDNWRPIHLACYQGHSEAVKLLIENKCDIEALDKQKQRPIHLACDQGHSEAVKLLIEHKCDIEALDKWNQRPIHLSCIQGHSEAVKLL 154          

HSP 2 Score: 147.132 bits (370), Expect = 6.434e-41
Identity = 77/118 (65.25%), Postives = 87/118 (73.73%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC  GH+E VKLLIEH CDIEALDKQKQRPIHLACYQGHSE VKLLIEH CD+EALDKQ   PIH  C  G+ E V+LLIE KCDI   D+    P+ +A    + E V++L
Sbjct:  235 RRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIENKCDIEVLDEWNRRPIHLACDQGHSEAVKLL 352          

HSP 3 Score: 144.05 bits (362), Expect = 6.447e-40
Identity = 75/125 (60.00%), Postives = 90/125 (72.00%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E  D   +RPIH AC+ GH+E VKLLIEH CDIEALDKQKQRPIHLACYQGHSE VKLLIE+ CD+E LD   ++PIH  C+ G+ E V+LLIE KCDI A DK    P+ +A    +   V++L
Sbjct:  261 EALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIENKCDIEVLDEWNRRPIHLACDQGHSEAVKLLIENKCDIEALDKWNQRPIHLACDQGHSGAVKLL 385          

HSP 4 Score: 142.51 bits (358), Expect = 2.247e-39
Identity = 80/143 (55.94%), Postives = 98/143 (68.53%), Query Frame = 3
Query:  138 SGSVELF--RRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            SG+V+L    +   E   E+K    RRPIHHAC  GH+E VKLLIE+ CDIEALDK  QRPIHLACYQGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A D     P+ +A    + E V++L
Sbjct:  379 SGAVKLLIENKCDIEALDEQK----RRPIHHACIQGHSEAVKLLIENKCDIEALDKWNQRPIHLACYQGHSEAVKLLIENKCDIEALDERNQRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLL 517          

HSP 5 Score: 137.887 bits (346), Expect = 8.960e-38
Identity = 70/117 (59.83%), Postives = 88/117 (75.21%), Query Frame = 3
Query:  210 RPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RPIH +C  GH+E VKLLIEHNCDIEALD + +RPIH+ACYQGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A DK +  P+ +A    + E V++L
Sbjct:  170 RPIHLSCIQGHSEAVKLLIEHNCDIEALDWRNRRPIHIACYQGHSEAVKLLIENKCDIEALDWWNRRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLL 286          

HSP 6 Score: 137.502 bits (345), Expect = 1.245e-37
Identity = 73/118 (61.86%), Postives = 86/118 (72.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC+ GH+E VKLLIE+ CDIEALD   +RPIHLAC QGHSE VKLLIEH CD+EALDKQ   PIH  C  G+ E V+LLIE KCDI A DK +  P+ +A    + E V++L
Sbjct:  202 RRPIHIACYQGHSEAVKLLIENKCDIEALDWWNRRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLL 319          

HSP 7 Score: 132.494 bits (332), Expect = 8.344e-36
Identity = 72/118 (61.02%), Postives = 84/118 (71.19%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC  GH+E VKLLIE+ CDIEALDK  QRPIHLAC QGHS  VKLLIE+ CD+EALD+Q   PIH  C  G+ E V+LLIE KCDI A DK    P+ +A    + E V++L
Sbjct:  334 RRPIHLACDQGHSEAVKLLIENKCDIEALDKWNQRPIHLACDQGHSGAVKLLIENKCDIEALDEQKRRPIHHACIQGHSEAVKLLIENKCDIEALDKWNQRPIHLACYQGHSEAVKLL 451          

HSP 8 Score: 132.109 bits (331), Expect = 1.032e-35
Identity = 69/118 (58.47%), Postives = 87/118 (73.73%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH AC+ GH+E VKLLIE+ CDIEALD++ QRPIHLAC QGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A D+    P+ +A      E V++L
Sbjct:  433 QRPIHLACYQGHSEAVKLLIENKCDIEALDERNQRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLLIENKCDIEALDERNQRPIHLACYYGRSEAVKLL 550          

HSP 9 Score: 131.339 bits (329), Expect = 2.214e-35
Identity = 73/125 (58.40%), Postives = 85/125 (68.00%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E  D   +RPIH AC  GH+E VKLLIEH CDIEALDK  QRPIHL+C QGHSE VKLLIE+ CD+EALDK   +PIH  C  G+ E V+LLIE  CDI A D     P+ IA    + E V++L
Sbjct:   96 EALDKQKQRPIHLACDQGHSEAVKLLIEHKCDIEALDKWNQRPIHLSCIQGHSEAVKLLIENKCDIEALDKRNWRPIHLSCIQGHSEAVKLLIEHNCDIEALDWRNRRPIHIACYQGHSEAVKLL 220          

HSP 10 Score: 130.568 bits (327), Expect = 3.166e-35
Identity = 68/118 (57.63%), Postives = 89/118 (75.42%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH AC  GH+E VKLLIE+ CDIEALD++ QRPIHLACY G SE VKLLIE+ C +EALDK+   PIH  C  G+ +  +LLIE KC+I+ARD+   +P++ A+K  N E+++IL
Sbjct:  499 QRPIHLACDQGHSEAVKLLIENKCDIEALDERNQRPIHLACYYGRSEAVKLLIENKCYIEALDKRNQRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKIL 616          

HSP 11 Score: 122.094 bits (305), Expect = 3.071e-32
Identity = 66/106 (62.26%), Postives = 77/106 (72.64%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIA 515
            +RPIH AC  GH+E VKLLIE+ CDIEALD + QRPIHLAC QGHSE VKLLIE+ CD+EALD   ++PIH  C  G  E V+LLIE KC I A DK    P+ +A
Sbjct:  466 QRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLLIENKCDIEALDERNQRPIHLACYYGRSEAVKLLIENKCYIEALDKRNQRPIHLA 571          

HSP 12 Score: 94.7449 bits (234), Expect = 1.152e-22
Identity = 48/87 (55.17%), Postives = 64/87 (73.56%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIE 458
            +RPIH AC+ G +E VKLLIE+ C IEALDK+ QRPIHLACY GHS+  KLLIEH C+++A D+    P+ +  + G  EI+++L E
Sbjct:  532 QRPIHLACYYGRSEAVKLLIENKCYIEALDKRNQRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKILNE 618          

HSP 13 Score: 54.299 bits (129), Expect = 8.771e-9
Identity = 31/80 (38.75%), Postives = 48/80 (60.00%), Query Frame = 3
Query:  135 RSGSVELF--RRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIE 368
            RS +V+L    + + E   +R     +RPIH AC+ GH++  KLLIEH C+I+A D+    P+  A   G++E +K+L E
Sbjct:  543 RSEAVKLLIENKCYIEALDKRN----QRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKILNE 618          
BLAST of SMED30016539 vs. Planmine SMEST
Match: SMESG000017277.1 (SMESG000017277.1)

HSP 1 Score: 212.616 bits (540), Expect = 3.855e-64
Identity = 109/154 (70.78%), Postives = 121/154 (78.57%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            MDEEFKLFINAI+SGS ELFRRYFKEEF ERKD DLRRPIHHAC  GHAEIVKLLIEH CDIEALDK   RPIHLACYQGHSE VKLLIE+ CD+EALDKQ   PIH  C+ G+ E V+LLIE KCDI A DK    P+ ++    + E V++L
Sbjct:    1 MDEEFKLFINAIKSGSAELFRRYFKEEFLERKDSDLRRPIHHACITGHAEIVKLLIEHKCDIEALDKDNWRPIHLACYQGHSEAVKLLIENKCDIEALDKQKQRPIHLACDQGHSEAVKLLIEHKCDIEALDKWNQRPIHLSCIQGHSEAVKLL 154          

HSP 2 Score: 146.747 bits (369), Expect = 8.707e-41
Identity = 77/118 (65.25%), Postives = 87/118 (73.73%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC  GH+E VKLLIEH CDIEALDKQKQRPIHLACYQGHSE VKLLIEH CD+EALDKQ   PIH  C  G+ E V+LLIE KCDI   D+    P+ +A    + E V++L
Sbjct:  235 RRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIENKCDIEVLDEWNRRPIHLACDQGHSEAVKLL 352          

HSP 3 Score: 143.665 bits (361), Expect = 8.791e-40
Identity = 75/125 (60.00%), Postives = 90/125 (72.00%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E  D   +RPIH AC+ GH+E VKLLIEH CDIEALDKQKQRPIHLACYQGHSE VKLLIE+ CD+E LD   ++PIH  C+ G+ E V+LLIE KCDI A DK    P+ +A    +   V++L
Sbjct:  261 EALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIENKCDIEVLDEWNRRPIHLACDQGHSEAVKLLIENKCDIEALDKWNQRPIHLACDQGHSGAVKLL 385          

HSP 4 Score: 142.124 bits (357), Expect = 3.061e-39
Identity = 80/143 (55.94%), Postives = 98/143 (68.53%), Query Frame = 3
Query:  138 SGSVELF--RRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            SG+V+L    +   E   E+K    RRPIHHAC  GH+E VKLLIE+ CDIEALDK  QRPIHLACYQGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A D     P+ +A    + E V++L
Sbjct:  379 SGAVKLLIENKCDIEALDEQK----RRPIHHACIQGHSEAVKLLIENKCDIEALDKWNQRPIHLACYQGHSEAVKLLIENKCDIEALDERNQRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLL 517          

HSP 5 Score: 137.502 bits (345), Expect = 1.206e-37
Identity = 70/117 (59.83%), Postives = 88/117 (75.21%), Query Frame = 3
Query:  210 RPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RPIH +C  GH+E VKLLIEHNCDIEALD + +RPIH+ACYQGHSE VKLLIE+ CD+EALD   ++PIH  C+ G+ E V+LLIE KCDI A DK +  P+ +A    + E V++L
Sbjct:  170 RPIHLSCIQGHSEAVKLLIEHNCDIEALDWRNRRPIHIACYQGHSEAVKLLIENKCDIEALDWWNRRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLL 286          

HSP 6 Score: 137.117 bits (344), Expect = 1.709e-37
Identity = 73/118 (61.86%), Postives = 86/118 (72.88%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC+ GH+E VKLLIE+ CDIEALD   +RPIHLAC QGHSE VKLLIEH CD+EALDKQ   PIH  C  G+ E V+LLIE KCDI A DK +  P+ +A    + E V++L
Sbjct:  202 RRPIHIACYQGHSEAVKLLIENKCDIEALDWWNRRPIHLACDQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLLIEHKCDIEALDKQKQRPIHLACYQGHSEAVKLL 319          

HSP 7 Score: 132.88 bits (333), Expect = 5.704e-36
Identity = 69/118 (58.47%), Postives = 89/118 (75.42%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH AC+ G +E VKLLIE+ C IEALDK  QRPIHLAC QGHSE VKLLIE+ CD+EALDK   +PIH  C  G+ +  +LLIE KC+I+ARD+   +P++ A+K  N E+++IL
Sbjct:  532 QRPIHLACYYGRSEAVKLLIENKCYIEALDKWNQRPIHLACIQGHSEAVKLLIENKCDIEALDKRNQRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKIL 649          

HSP 8 Score: 132.109 bits (331), Expect = 1.133e-35
Identity = 72/118 (61.02%), Postives = 84/118 (71.19%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDKQ---PIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            RRPIH AC  GH+E VKLLIE+ CDIEALDK  QRPIHLAC QGHS  VKLLIE+ CD+EALD+Q   PIH  C  G+ E V+LLIE KCDI A DK    P+ +A    + E V++L
Sbjct:  334 RRPIHLACDQGHSEAVKLLIENKCDIEALDKWNQRPIHLACDQGHSGAVKLLIENKCDIEALDEQKRRPIHHACIQGHSEAVKLLIENKCDIEALDKWNQRPIHLACYQGHSEAVKLL 451          

HSP 9 Score: 130.954 bits (328), Expect = 3.034e-35
Identity = 73/125 (58.40%), Postives = 85/125 (68.00%), Query Frame = 3
Query:  186 ERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK---QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            E  D   +RPIH AC  GH+E VKLLIEH CDIEALDK  QRPIHL+C QGHSE VKLLIE+ CD+EALDK   +PIH  C  G+ E V+LLIE  CDI A D     P+ IA    + E V++L
Sbjct:   96 EALDKQKQRPIHLACDQGHSEAVKLLIEHKCDIEALDKWNQRPIHLSCIQGHSEAVKLLIENKCDIEALDKRNWRPIHLSCIQGHSEAVKLLIEHNCDIEALDWRNRRPIHIACYQGHSEAVKLL 220          

HSP 10 Score: 125.561 bits (314), Expect = 2.298e-33
Identity = 69/118 (58.47%), Postives = 83/118 (70.34%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH AC  GH+E VKLLIE+ CDIEALD + QRPIHLAC QGHSE VKLLIE+ CD+EALD   ++PIH  C  G  E V+LLIE KC I A DK    P+ +A    + E V++L
Sbjct:  466 QRPIHLACDQGHSEAVKLLIENKCDIEALDDRNQRPIHLACDQGHSEAVKLLIENKCDIEALDERNQRPIHLACYYGRSEAVKLLIENKCYIEALDKWNQRPIHLACIQGHSEAVKLL 583          

HSP 11 Score: 53.9138 bits (128), Expect = 1.239e-8
Identity = 25/54 (46.30%), Postives = 37/54 (68.52%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIE 368
            +RPIH AC+ GH++  KLLIEH C+I+A D+    P+  A   G++E +K+L E
Sbjct:  598 QRPIHLACYYGHSKAFKLLIEHKCEIDARDESGNSPMEFAMKFGNTEIIKILNE 651          
BLAST of SMED30016539 vs. Planmine SMEST
Match: SMESG000017261.1 (SMESG000017261.1)

HSP 1 Score: 205.297 bits (521), Expect = 1.981e-63
Identity = 105/154 (68.18%), Postives = 117/154 (75.97%), Query Frame = 3
Query:   99 MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALD---KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            MDEEFKLFINAI+SGS  LFRRYFKEE+ ERKD DL  PIHHACFAGHAEIVKLLIEHNCDIEALDK   RPIH ACYQGHSE VKLLIEH CD+EA D   ++PIH+ C  G  E V+LLIE KCDI A D+    P+  A    + E V++L
Sbjct:    1 MDEEFKLFINAIKSGSAVLFRRYFKEEYLERKDADLWSPIHHACFAGHAEIVKLLIEHNCDIEALDKWNLRPIHYACYQGHSEAVKLLIEHKCDIEAFDWWNQRPIHYACYQGRSEAVKLLIEHKCDIEAIDEWNQRPIHHACDQGHSEAVKLL 154          

HSP 2 Score: 133.65 bits (335), Expect = 1.332e-36
Identity = 76/170 (44.71%), Postives = 97/170 (57.06%), Query Frame = 3
Query:  207 RRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEH----------------------------------------NCDMEALD---------------KQPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEIL 551
            +RPIH+AC+ G +E VKLLIEH CDIEA+D+  QRPIH AC QGHSE VKLLIE+                                        N D++ +                ++PIHW CESG I++V+ LI+ KCDI+ARDK  ++PLDIAIKN+NKELVE+L
Sbjct:  103 QRPIHYACYQGRSEAVKLLIEHKCDIEAIDEWNQRPIHHACDQGHSEAVKLLIENKCDIEALDESGDSPMEIAVKSHNERIIHILNGPCLLRACKNGDLKKVSTYLNGAAIEYCDKDQRRPIHWACESGNIKVVKSLIKTKCDISARDKSNLSPLDIAIKNQNKELVELL 272          

HSP 3 Score: 55.0694 bits (131), Expect = 5.401e-9
Identity = 30/81 (37.04%), Postives = 44/81 (54.32%), Query Frame = 3
Query:  120 FINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAEIVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLL 362
             + A ++G ++    Y      E  D D RRPIH AC +G+ ++VK LI+  CDI A DK    P+ +A    + E V+LL
Sbjct:  192 LLRACKNGDLKKVSTYLNGAAIEYCDKDQRRPIHWACESGNIKVVKSLIKTKCDISARDKSNLSPLDIAIKNQNKELVELL 272          
The following BLAST results are available for this feature:
BLAST of SMED30016539 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANKRD282.313e-2040.37ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:H... [more]
ANKRD282.811e-2040.37ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:H... [more]
ANKRD282.811e-2040.37ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:H... [more]
ANKRD503.324e-1631.34ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:H... [more]
ANKRD503.862e-1631.34ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of SMED30016539 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
unc-441.113e-1330.00AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.113e-1330.00AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.359e-1330.00AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.359e-1330.00AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.362e-1330.00AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
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BLAST of SMED30016539 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG111684.862e-1528.28gene:FBgn0039249 transcript:FBtr0306089[more]
CG43932.368e-1430.51gene:FBgn0039075 transcript:FBtr0339617[more]
CG43932.368e-1430.51gene:FBgn0039075 transcript:FBtr0301085[more]
CG43932.368e-1430.51gene:FBgn0039075 transcript:FBtr0339616[more]
CG426721.251e-1334.48gene:FBgn0261554 transcript:FBtr0302702[more]
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BLAST of SMED30016539 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1b1.745e-2031.03ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b1.936e-2031.03ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b3.124e-2031.03ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b4.014e-2031.03ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b4.165e-2031.03ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of SMED30016539 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rev15.733e-1938.53REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
rev15.754e-1938.53REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
rev16.502e-1938.53REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
rev16.568e-1938.53REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
rev16.589e-1938.53REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
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BLAST of SMED30016539 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ankrd281.736e-2040.37ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MG... [more]
Ankrd282.112e-2040.37ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MG... [more]
Gabpb13.751e-1730.20GA repeat binding protein, beta 1 [Source:MGI Symb... [more]
Gabpb13.443e-1630.20GA repeat binding protein, beta 1 [Source:MGI Symb... [more]
Gabpb13.902e-1630.20GA repeat binding protein, beta 1 [Source:MGI Symb... [more]
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BLAST of SMED30016539 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q505D1|ANR28_MOUSE1.214e-1940.37Serine/threonine-protein phosphatase 6 regulatory ... [more]
sp|O15084|ANR28_HUMAN1.350e-1940.37Serine/threonine-protein phosphatase 6 regulatory ... [more]
sp|Q9ULJ7|ANR50_HUMAN1.596e-1531.34Ankyrin repeat domain-containing protein 50 OS=Hom... [more]
sp|Q1RMI3|GABP1_BOVIN2.293e-1530.20GA-binding protein subunit beta-1 OS=Bos taurus OX... [more]
sp|Q06547|GABP1_HUMAN2.676e-1530.20GA-binding protein subunit beta-1 OS=Homo sapiens ... [more]
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BLAST of SMED30016539 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V4K3J31.027e-2341.38Ankyrin repeat OS=uncultured Acidilobus sp. JCHS O... [more]
V4JNT41.963e-2340.52Ankyrin repeat OS=uncultured Acidilobus sp. OSP8 O... [more]
V4JZM12.324e-2239.66Ankyrin repeat OS=uncultured Acidilobus sp. MG OX=... [more]
A0A1V4WVK32.972e-2237.93Ankyrin repeat protein OS=Syntrophorhabdus sp. Pta... [more]
A0A497XRJ55.572e-2233.73Ankyrin repeat protein OS=Hydrogenivirga caldilito... [more]
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BLAST of SMED30016539 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1b4.960e-1932.00ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
gabpb12.668e-1831.54GA binding protein transcription factor subunit be... [more]
psmd103.951e-1833.33proteasome 26S subunit, non-ATPase 10 [Source:NCBI... [more]
ank1a2.521e-1731.20ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
psmd101.014e-1632.79proteasome 26S subunit, non-ATPase 10 [Source:NCBI... [more]
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BLAST of SMED30016539 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000003991.13.555e-1938.53pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1... [more]
ENSPMAT00000007954.18.878e-1834.75pep scaffold:Pmarinus_7.0:GL479092:583:36301:1 gen... [more]
ankrd1a1.746e-1630.83ankyrin repeat domain 1a (cardiac muscle) [Source:... [more]
ENSPMAT00000007449.12.750e-1427.59pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1... [more]
fem1c3.264e-1434.48fem-1 homolog c [Source:NCBI gene;Acc:378965][more]
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BLAST of SMED30016539 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
NAS62.305e-627.13Evolutionarily conserved 19S regulatory particle a... [more]
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BLAST of SMED30016539 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO308896.509e-1435.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387021.837e-1333.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO411014.421e-1034.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO411044.421e-1034.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO280235.600e-1032.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30016539 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ankrd28b9.539e-2040.37ankyrin repeat domain 28 [Source:NCBI gene;Acc:101... [more]
ankrd28b1.048e-1940.37ankyrin repeat domain 28 [Source:NCBI gene;Acc:101... [more]
ankrd28b1.388e-1940.37ankyrin repeat domain 28 [Source:NCBI gene;Acc:101... [more]
ank1a1.726e-1731.03ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ENSORLT00000028696.16.860e-1737.61serine/threonine-protein phosphatase 6 regulatory ... [more]
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BLAST of SMED30016539 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017277.11.477e-6470.78SMESG000017277.1[more]
SMESG000017277.12.015e-6470.78SMESG000017277.1[more]
SMESG000017277.12.211e-6470.78SMESG000017277.1[more]
SMESG000017277.13.855e-6470.78SMESG000017277.1[more]
SMESG000017261.11.981e-6368.18SMESG000017261.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016539 ID=SMED30016539|Name=SMED30016539|organism=Schmidtea mediterranea sexual|type=transcript|length=554bp
ACTAGTGAAATATCAAATTTCGGAATTATTTTTGTTGTTTAGACATTATT
TGTTGAAATTTACTATTTGTTCTAATTGGAGAAATATTTTCGTAATCGAT
GGATGAGGAATTCAAATTATTTATCAACGCAATCAGATCTGGATCCGTAG
AATTATTCCGCAGATATTTCAAAGAGGAATTTTCGGAGCGAAAGGATGTC
GATTTGAGGCGTCCGATTCATCACGCATGCTTTGCAGGACACGCTGAAAT
TGTAAAGCTGCTGATTGAGCACAATTGCGATATCGAGGCGCTTGATAAGC
AGAAACAGCGGCCGATTCATCTCGCATGCTATCAAGGGCACTCAGAGCCA
GTCAAACTGCTGATTGAGCACAATTGTGATATGGAGGCGCTTGATAAGCA
GCCAATTCATTGGGAATGTGAGAGCGGCTATATTGAAATTGTCGAATTGC
TGATTGAAAGAAAATGTGATATTAATGCAAGGGATAAATGTGAGATGAAC
CCTCTGGACATCGCTATAAAGAATGAAAATAAGGAGCTAGTTGAGATCCT
CGGG
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protein sequence of SMED30016539-orf-1

>SMED30016539-orf-1 ID=SMED30016539-orf-1|Name=SMED30016539-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=152bp
MDEEFKLFINAIRSGSVELFRRYFKEEFSERKDVDLRRPIHHACFAGHAE
IVKLLIEHNCDIEALDKQKQRPIHLACYQGHSEPVKLLIEHNCDMEALDK
QPIHWECESGYIEIVELLIERKCDINARDKCEMNPLDIAIKNENKELVEI
LG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000035Category 3 cell
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003824catalytic activity
Vocabulary: biological process
TermDefinition
GO:0009116nucleoside metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 35..64
e-value: 1.2E-5
score: 34.7
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 35..67
score: 11.487
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 68..88
score: 9.084
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 31..88
e-value: 2.2E-12
score: 47.4
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 35..88
score: 19.067
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 2..88
e-value: 6.5E-21
score: 76.3
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 8..88
NoneNo IPR availablePANTHERPTHR24198:SF165ANKYRIN REPEAT FAMILY PROTEINcoord: 31..87
coord: 27..70
NoneNo IPR availablePANTHERPTHR24198ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEINcoord: 31..87
coord: 27..70