SMED30016537

Overview
NameSMED30016537
Smed IDSMED30016537
Length (bp)930
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016537 (SMED30016537) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016537 (SMED30016537) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016537 (SMED30016537) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016537 (SMED30016537) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30016537

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30016537h1SMcG0019890 SmedASXL_001369SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30016537h1SMcG0019890 SmedASXL_001369SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30016537h1SMcG0019890 dd_Smed_v4_16549_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30016537h1SMcG0019890 dd_Smed_v4_16549_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016537h1SMcG0019890 SMED_18010_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30016537h1SMcG0019890 SMED_18010_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016537 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001496:45334..46263 +4650
Homology
BLAST of SMED30016537 vs. Planmine SMEST
Match: SMESG000072339.1 (SMESG000072339.1)

HSP 1 Score: 575.089 bits (1481), Expect = 0.000e+0
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 1
Query:   25 MDINIFDALNYLTGSSKTSARFKLCDFNTITDLLSFNDTSIDKMEEYIQLQCSLMDLAERSIYLDGDDIQNPKCFMISPGLRNRICKAVSTIVNSMNGASLSLSQKLLNHNFIDYGFKDNIVSVKNIKPLSITDNMFMNQKSFNSTNIQTKALATSKNNLANISKLYTPXXXXXXXXXXXXLDNPIARGKKVDQKLIELVTENFYHTIANDQPLNFDNCHLIPKKDFDIKINLNYKNFQNSKFYYVCNLCNGKLVKFNLNKDLKPIQTGIKSHNRTHFPTKLTFIPTNFRRSSLNIEE 918
            MDINIFDALNYLTGSSKTSARFKLCDFNTITDLLSFNDTSIDKMEEYIQLQCSLMDLAERSIYLDGDDIQNPKCFMISPGLRNRICKAVSTIVNSMNGASLSLSQKLLNHNFIDYGFKDNIVSVKNIKPLSITDNMFMNQKSFNSTNIQTKALATSKNNLANISKLYTPINSKKIVKSSNKLDNPIARGKKVDQKLIELVTENFYHTIANDQPLNFDNCHLIPKKDFDIKINLNYKNFQNSKFYYVCNLCNGKLVKFNLNKDLKPIQTGIKSHNRTHFPTKLTFIPTNFRRSSLNIEE
Sbjct:    1 MDINIFDALNYLTGSSKTSARFKLCDFNTITDLLSFNDTSIDKMEEYIQLQCSLMDLAERSIYLDGDDIQNPKCFMISPGLRNRICKAVSTIVNSMNGASLSLSQKLLNHNFIDYGFKDNIVSVKNIKPLSITDNMFMNQKSFNSTNIQTKALATSKNNLANISKLYTPINSKKIVKSSNKLDNPIARGKKVDQKLIELVTENFYHTIANDQPLNFDNCHLIPKKDFDIKINLNYKNFQNSKFYYVCNLCNGKLVKFNLNKDLKPIQTGIKSHNRTHFPTKLTFIPTNFRRSSLNIEE 298          
BLAST of SMED30016537 vs. Planmine SMEST
Match: SMESG000072330.1 (SMESG000072330.1)

HSP 1 Score: 114.775 bits (286), Expect = 8.754e-30
Identity = 97/299 (32.44%), Postives = 145/299 (48.49%), Query Frame = 1
Query:   25 MDINIFDALNYLTGSSKTSARFKLCDFNTITDLLSFNDTSIDKMEEYIQLQCSLMDLAERSIYLDGDDIQNPKCFMISPGLRNRICKAVSTIVNSMNGASL--SLSQKLLNHNFI-------DYG--FKDNIVSVKNIKPLSITDNMFMNQKSFNSTNI-QT----KALATSKNN-----LANISKLYTPXXXXXXXXXXXXLDNPIARGK-KVDQKLIELVTENFYHTIANDQPLNFDNCHLIPKKDFDIKINLNYKNFQNSKFYYVCNLCNGKLVKFNLNKDLKPIQTGIKSHNRTH 855
            M+I+I +A+ YL    K +   +LC F  + D L+FN+ ++DK+E+YIQ+QCSLM +  R +YL+  D+ +P  F+ISPGLRNR+ KAV  +V+     SL  S+ +     N +       D G    +N++   NI  +S  D+     K + STN  QT      L    NN     LAN SK                 D  +   K  +DQ       E+F   +  +   N     L+PK++FD++ + N  +  NS   Y+C  CN K +KF     LK +   IKSH + H
Sbjct:    1 MNIDIPNAIAYLCEFPKVANWLELCKFELMDDFLNFNNNTVDKIEKYIQIQCSLMPINVRQLYLNESDLLSPTKFLISPGLRNRLLKAVDILVSYFEINSLNNSIHKSFCMENMLYQKLSETDSGNNTNNNLMFSANILDISENDSRISAPKHYESTNSSQTLGYESTLEVINNNDSEICLANQSK-----------------DLQVGEFKINIDQYF-----EDFVEVLKRELDFN-----LVPKENFDLEFHFNSDSISNSYGNYICYYCNKKKLKFKFTAMLKVVYGNIKSHLKKH 272          
BLAST of SMED30016537 vs. Planmine SMEST
Match: SMESG000072336.1 (SMESG000072336.1)

HSP 1 Score: 115.931 bits (289), Expect = 1.844e-28
Identity = 92/309 (29.77%), Postives = 149/309 (48.22%), Query Frame = 1
Query:   34 NIFDALNYLTGSSKTSARFKLCDFNTITDLLSFNDTSIDKMEEYIQLQCSLMDLAERSIYLDGDDIQNPKCFMISPGLRNRICKAVSTIVNSMNGASLSLSQKLLNHNFIDYGFKDNIVSVKNIKPLSITDNMFMNQKSFNSTNIQTKALATSKNNLANISKLYTPXXXXXXXXXXXXLDNPIARGKKVDQKLIELVTEN-------FYHTIANDQPLNFDNCHLI-----PKKDFDIKINLNYKNFQNSKFYYVCNLCNGKLVKFNLNKDLKPIQTGIKSHNRTH--FPTKLTFIPTNFRRSSLNIEE 918
            N FD ++ LTG  K     KLC F +I D ++FND SID ++EY+++ CS+M + ER +YLD DDI NP+ + I+PG+RNR+ KAV+ I+ S+ G +   S  L NH                 K   + +N F N    N+         +S   +    +L +     K +   ++L        K     +E  TE+       F+  + N+  L F N H +     PK  FD++ ++   + + S  Y++C  CN K +KF+ +  +      IKSH +TH  +      IP   R++++N E+
Sbjct:    8 NFFDVISILTGRPKLVQCLKLCKFRSILDFINFNDKSIDNVQEYVRIICSIMSVEERKMYLDEDDIPNPQDYFITPGIRNRLLKAVTVILASI-GINKVKSNDLFNHK-------------TPPKRFELENNDFPNS---NALEFSPSKKPSSSPQVKYRKRLISKSIESKEIGYEHRL-------SKASSNCVESTTEDPIDEFRGFFKHVRNNL-LGFYNEHKMESKHKPKIHFDLEFHIE-NDTKKSFGYFICFNCNKKKLKFSFSSKMVISFGSIKSHLKTHHDYVANNNNIPQK-RKNAINGEQ 289          
BLAST of SMED30016537 vs. Planmine SMEST
Match: SMESG000002170.1 (SMESG000002170.1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.071e-16
Identity = 74/281 (26.33%), Postives = 120/281 (42.70%), Query Frame = 1
Query:   37 IFDALNYLTGSSKTSARFKLCDFNTITDLLSFNDTSIDKMEEYIQLQCSLMDLAERSIYLDGDDIQNPKCFMISPGLRNRICKAVSTIVNSMNGASLSLSQKLLNHNFIDYGFKDNIVSVKNIKPLSITDNMFMNQKSFNSTNIQTKALATSKNNLANISKLYTPXXXXXXXXXXXXLDNPIARGKKVDQKLIELVTENFYHTIANDQPLNFDNCHLIPKKDFDIKINLNYKNFQNSKFYYVCNLCN-------GKLVKFNLNKDLKPIQTGIKSHNRTHF 858
            ++   N   GSSK SA   +C +  +    SFN  S+  ME+YI   C LM+  ER  Y+D   I NPK F+  P   + I K V++I   ++  ++ +     N N  +    D++  +  I+      N+      F S         T  N ++  S   +P+                 R K++D+       + FY +I +    +F NC+L P  DF++++        NSK Y++C LCN       G +V+F++      I   IK H   HF
Sbjct:    4 LYKIFNNKLGSSKISACLAICGYTEMQHFASFNTESLANMEKYIGKACFLMNKEERKKYIDEIFIDNPKEFIFPPRCTDEILKIVASIKEYLDMNNVPMK----NTNISNSDLDDSLKLIPKIEKSDYFINLKQQSHLFKSIISPIMLNETISNEMSINSCCSSPVCE--------------ERIKEIDR------LKLFYQSIKDRSNRHFLNCNLEPNIDFEVQLYKVDDVLYNSKAYFICKLCNKIRKGQSGVVVRFDITPSGNVIFRNIKIHLAKHF 260          
BLAST of SMED30016537 vs. Planmine SMEST
Match: SMESG000026581.1 (SMESG000026581.1)

HSP 1 Score: 72.4034 bits (176), Expect = 4.059e-14
Identity = 35/87 (40.23%), Postives = 58/87 (66.67%), Query Frame = 1
Query:   49 LNYLTGSSKTSARFKLCDFNTITDLLSFNDTSIDKMEEYIQLQCSLMDLAERSIYLDGDDIQNPKCFMISPGLRNRI---CKAVSTI 300
            ++ ++GS K  A FK+ +   ++D+++ ++ +I  ME++IQ +CS + L+ERS +LD   + NP+ FMI PGL  RI   C+ VSTI
Sbjct:    9 ISVISGSDKLEACFKVSEIYNVSDVMNIDEKTIQHMEKFIQKKCSNIPLSERSKFLDDSLLNNPEMFMIPPGLTQRIINNCQKVSTI 95          
The following BLAST results are available for this feature:
BLAST of SMED30016537 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016537 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000072339.10.000e+0100.00SMESG000072339.1[more]
SMESG000072330.18.754e-3032.44SMESG000072330.1[more]
SMESG000072336.11.844e-2829.77SMESG000072336.1[more]
SMESG000002170.11.071e-1626.33SMESG000002170.1[more]
SMESG000026581.14.059e-1440.23SMESG000026581.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016537 ID=SMED30016537|Name=SMED30016537|organism=Schmidtea mediterranea sexual|type=transcript|length=930bp
AAATAATCAAATAAAGTTTTTAAAATGGATATTAATATTTTTGATGCATT
AAATTATTTAACCGGCTCCTCAAAAACTAGTGCTAGATTTAAACTTTGCG
ATTTTAATACAATTACAGACTTACTGAGTTTCAATGACACTTCCATTGAT
AAAATGGAAGAATACATACAACTTCAATGTTCGTTGATGGATTTAGCAGA
AAGATCTATTTATTTAGATGGAGATGATATACAGAACCCAAAATGTTTTA
TGATTTCTCCTGGATTACGTAATCGAATATGTAAGGCTGTTTCAACAATT
GTTAATTCTATGAATGGAGCTTCATTGTCTTTATCTCAAAAGTTATTAAA
CCATAATTTCATTGATTATGGGTTCAAGGATAATATTGTTAGTGTGAAAA
ATATCAAACCATTAAGCATTACTGATAATATGTTTATGAATCAGAAGAGT
TTTAATTCCACAAATATTCAGACTAAAGCTTTAGCCACTAGTAAAAATAA
TTTAGCGAATATTTCTAAATTATATACGCCGATCAATTCAAAAAAGATTG
TTAAATCGAGTAATAAATTGGATAATCCAATTGCTCGTGGTAAAAAAGTT
GATCAAAAGCTTATCGAACTTGTAACAGAAAATTTTTATCATACAATTGC
CAATGACCAACCATTAAACTTTGACAATTGTCACCTTATTCCAAAAAAAG
ATTTTGATATTAAGATTAATTTAAATTATAAGAACTTTCAGAATTCGAAA
TTTTATTATGTATGTAATTTGTGCAATGGAAAACTTGTAAAATTTAATTT
GAATAAAGATTTAAAACCAATTCAGACCGGCATTAAATCACATAATCGAA
CTCATTTTCCTACCAAATTGACATTTATTCCGACTAATTTTAGGAGAAGT
AGTTTAAATATTGAAGAATAAATTATCTGG
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protein sequence of SMED30016537-orf-1

>SMED30016537-orf-1 ID=SMED30016537-orf-1|Name=SMED30016537-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=299bp
MDINIFDALNYLTGSSKTSARFKLCDFNTITDLLSFNDTSIDKMEEYIQL
QCSLMDLAERSIYLDGDDIQNPKCFMISPGLRNRICKAVSTIVNSMNGAS
LSLSQKLLNHNFIDYGFKDNIVSVKNIKPLSITDNMFMNQKSFNSTNIQT
KALATSKNNLANISKLYTPINSKKIVKSSNKLDNPIARGKKVDQKLIELV
TENFYHTIANDQPLNFDNCHLIPKKDFDIKINLNYKNFQNSKFYYVCNLC
NGKLVKFNLNKDLKPIQTGIKSHNRTHFPTKLTFIPTNFRRSSLNIEE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell