SMED30016491

Overview
NameSMED30016491
Smed IDSMED30016491
Length (bp)633
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016491 (SMED30016491) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016491 (SMED30016491) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016491 (SMED30016491) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016491 (SMED30016491) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016491

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 72

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30016491h1SMcG0020223 SmedASXL_023006SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30016491h1SMcG0020223 SmedASXL_003310SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig10023uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig10023newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig45334newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig45334uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig15727uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig15727newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig38190newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig38190uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig18341newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig18341uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig20965newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig20965uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig9118uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig9118newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig45585newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig45585uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig7809uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig7809newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig48632newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016491h1SMcG0020223 Contig48632uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30016491h1SMcG0020223 dd_Smed_v4_3767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30016491h1SMcG0020223 dd_Smed_v4_3767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016491h1SMcG0020223 dd_Smed_v4_3767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016491h1SMcG0020223 dd_Smed_v4_3767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016491h1SMcG0020223 dd_Smed_v4_3767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016491h1SMcG0020223 dd_Smed_v4_3767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30016491h1SMcG0020223 dd_Smed_v4_3767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30016491h1SMcG0020223 dd_Smed_v4_655_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30016491h1SMcG0020223 dd_Smed_v6_655_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
enteric muscle cellSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30016491h1SMcG0020223 dd_Smed_v6_655_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
oocyteSMED30016491h1SMcG0020223 Contig20965newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30016491h1SMcG0020223 Contig20965uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30016491h1SMcG0020223 Contig20965newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30016491h1SMcG0020223 Contig20965uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
non-ciliated epidermisSMED30016491h1SMcG0020223 dd_Smed_v4_19738_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
non-ciliated epidermisSMED30016491h1SMcG0020223 dd_Smed_v4_1205_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016491h1SMcG0020223 SMED_02081_V2_1rGPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016491 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.007745:3803..4435 -3129
Homology
[Close]
BLAST of SMED30016491 vs. Planmine SMEST
Match: SMESG000043799.1 (SMESG000043799.1)

HSP 1 Score: 351.673 bits (901), Expect = 7.762e-112
Identity = 207/210 (98.57%), Postives = 210/210 (100.00%), Query Frame = 3 
Query:    3 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAKTGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLXXXXXXXXXXXXEEIMKNKRQININKKVEKQLETSYXXXXXXXXXXXXXXXSQSDMDNRGQNISALLTNEKERI 632
            IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMA+TGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYN+NRLKYEERKRKRKSQSDMDNRGQNISALLT+EKERI
Sbjct: 2752 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAETGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYNENRLKYEERKRKRKSQSDMDNRGQNISALLTSEKERI 2961
[Close]
BLAST of SMED30016491 vs. Planmine SMEST
Match: SMESG000043799.1 (SMESG000043799.1)

HSP 1 Score: 351.673 bits (901), Expect = 7.917e-112
Identity = 207/210 (98.57%), Postives = 210/210 (100.00%), Query Frame = 3 
Query:    3 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAKTGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLXXXXXXXXXXXXEEIMKNKRQININKKVEKQLETSYXXXXXXXXXXXXXXXSQSDMDNRGQNISALLTNEKERI 632
            IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMA+TGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYN+NRLKYEERKRKRKSQSDMDNRGQNISALLT+EKERI
Sbjct: 2752 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAETGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYNENRLKYEERKRKRKSQSDMDNRGQNISALLTSEKERI 2961
[Close]
BLAST of SMED30016491 vs. Planmine SMEST
Match: SMESG000043799.1 (SMESG000043799.1)

HSP 1 Score: 351.673 bits (901), Expect = 7.994e-112
Identity = 207/210 (98.57%), Postives = 210/210 (100.00%), Query Frame = 3 
Query:    3 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAKTGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLXXXXXXXXXXXXEEIMKNKRQININKKVEKQLETSYXXXXXXXXXXXXXXXSQSDMDNRGQNISALLTNEKERI 632
            IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMA+TGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYN+NRLKYEERKRKRKSQSDMDNRGQNISALLT+EKERI
Sbjct: 2752 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAETGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYNENRLKYEERKRKRKSQSDMDNRGQNISALLTSEKERI 2961
[Close]
BLAST of SMED30016491 vs. Planmine SMEST
Match: SMESG000043799.1 (SMESG000043799.1)

HSP 1 Score: 351.673 bits (901), Expect = 8.229e-112
Identity = 207/210 (98.57%), Postives = 210/210 (100.00%), Query Frame = 3 
Query:    3 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAKTGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLXXXXXXXXXXXXEEIMKNKRQININKKVEKQLETSYXXXXXXXXXXXXXXXSQSDMDNRGQNISALLTNEKERI 632
            IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMA+TGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYN+NRLKYEERKRKRKSQSDMDNRGQNISALLT+EKERI
Sbjct: 2752 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAETGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYNENRLKYEERKRKRKSQSDMDNRGQNISALLTSEKERI 2961
[Close]
BLAST of SMED30016491 vs. Planmine SMEST
Match: SMESG000043799.1 (SMESG000043799.1)

HSP 1 Score: 351.673 bits (901), Expect = 8.468e-112
Identity = 207/210 (98.57%), Postives = 210/210 (100.00%), Query Frame = 3 
Query:    3 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAKTGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLXXXXXXXXXXXXEEIMKNKRQININKKVEKQLETSYXXXXXXXXXXXXXXXSQSDMDNRGQNISALLTNEKERI 632
            IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMA+TGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYN+NRLKYEERKRKRKSQSDMDNRGQNISALLT+EKERI
Sbjct: 2752 IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIEQTEKSERKINLIKAMKEKLINPEKMTIKMAETGIIKCLYEALEENLIDEEQTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEEIMKNKRQININKKVEKQLETSYNENRLKYEERKRKRKSQSDMDNRGQNISALLTSEKERI 2961
The following BLAST results are available for this feature:
BLAST of SMED30016491 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016491 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000043799.17.762e-11298.57SMESG000043799.1[more]
SMESG000043799.17.917e-11298.57SMESG000043799.1[more]
SMESG000043799.17.994e-11298.57SMESG000043799.1[more]
SMESG000043799.18.229e-11298.57SMESG000043799.1[more]
SMESG000043799.18.468e-11298.57SMESG000043799.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016491 ID=SMED30016491|Name=SMED30016491|organism=Schmidtea mediterranea sexual|type=transcript|length=633bp
ATATAATTAGTGATAAATATAAATTATTAAATAAACAAAAAATGAAAGAA
AGTGAAAATATTTTATCATCTTTTTTATATTCATATATTCCGATCCAAGA
CACTTCAGCGGAAATATTATTTGCTAATGAAGCTCCAAATATAATTATTG
AGCAAACCGAAAAATCGGAAAGGAAAATAAACTTAATAAAGGCTATGAAA
GAAAAACTAATAAATCCAGAAAAAATGACGATCAAAATGGCTAAAACTGG
AATAATAAAATGTTTATATGAAGCATTAGAGGAAAATCTTATTGATGAAG
AGCAAACATTATTTCGAGAAATACGAAGTGGAAATATTGTCTCATTACAA
ATGTTATTAGGCAACAATATACCTTTCAGAGACATTATAATGTTTACACA
TGACTACTTAAAAAAAGATAATAAAAGCAAACAAAATAAAAATCAAGAAG
AAATAATGAAAAATAAAAGACAAATAAATATAAACAAAAAGGTCGAAAAA
CAATTAGAAACAAGCTACAACAAAAACAGATTAAAATATGAAGAAAGAAA
AAGAAAAAGAAAATCTCAATCTGATATGGATAATCGAGGACAAAATATAA
GTGCTTTGCTAACAAATGAAAAAGAACGTATAG
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protein sequence of SMED30016491-orf-1

>SMED30016491-orf-1 ID=SMED30016491-orf-1|Name=SMED30016491-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=210bp
IISDKYKLLNKQKMKESENILSSFLYSYIPIQDTSAEILFANEAPNIIIE
QTEKSERKINLIKAMKEKLINPEKMTIKMAKTGIIKCLYEALEENLIDEE
QTLFREIRSGNIVSLQMLLGNNIPFRDIIMFTHDYLKKDNKSKQNKNQEE
IMKNKRQININKKVEKQLETSYNKNRLKYEERKRKRKSQSDMDNRGQNIS
ALLTNEKERI
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0000231vitelline gland
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR035915Plakin_repeat_sf
IPR001101Plectin_repeat
Vocabulary: cellular component
TermDefinition
GO:0005856cytoskeleton
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..210
IPR035915Plakin repeat superfamilySUPERFAMILYSSF75399Plakin repeatcoord: 54..122