SMED30016487

Overview
NameSMED30016487
Smed IDSMED30016487
Length (bp)465
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016487 (SMED30016487) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016487 (SMED30016487) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016487 (SMED30016487) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016487 (SMED30016487) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016487

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30016487h1SMcG0022777 dd_Smed_v4_4464_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016487h1SMcG0022777 dd_Smed_v4_4464_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016487h1SMcG0022777 dd_Smed_v4_4464_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30016487h1SMcG0022777 dd_Smed_v4_4464_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30016487h1SMcG0022777 dd_Smed_v4_4464_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016487 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.012418:6640..15295 -2297
Homology
[Close]
BLAST of SMED30016487 vs. Ensembl Human
Match: PRKCA (protein kinase C alpha [Source:HGNC Symbol;Acc:HGNC:9393])

HSP 1 Score: 203.371 bits (516), Expect = 1.979e-65
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GPDT + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   20 FARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 133
[Close]
BLAST of SMED30016487 vs. Ensembl Human
Match: PRKCA (protein kinase C alpha [Source:HGNC Symbol;Acc:HGNC:9393])

HSP 1 Score: 204.912 bits (520), Expect = 1.296e-62
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GPDT + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   20 FARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 133
[Close]
BLAST of SMED30016487 vs. Ensembl Human
Match: PRKCB (protein kinase C beta [Source:HGNC Symbol;Acc:HGNC:9395])

HSP 1 Score: 200.29 bits (508), Expect = 6.509e-61
Identity = 92/120 (76.67%), Postives = 102/120 (85.00%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            + S   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KCD
Sbjct:   14 EESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCD 133

HSP 2 Score: 46.9802 bits (110), Expect = 3.925e-6
Identity = 21/50 (42.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   VHKRC   V   C
Sbjct:  102 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLC 151
[Close]
BLAST of SMED30016487 vs. Ensembl Human
Match: PRKCB (protein kinase C beta [Source:HGNC Symbol;Acc:HGNC:9395])

HSP 1 Score: 200.29 bits (508), Expect = 7.432e-61
Identity = 92/120 (76.67%), Postives = 102/120 (85.00%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            + S   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KCD
Sbjct:   14 EESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCD 133

HSP 2 Score: 46.9802 bits (110), Expect = 4.003e-6
Identity = 21/50 (42.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   VHKRC   V   C
Sbjct:  102 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLC 151
[Close]
BLAST of SMED30016487 vs. Ensembl Human
Match: PRKCG (protein kinase C gamma [Source:HGNC Symbol;Acc:HGNC:9402])

HSP 1 Score: 185.267 bits (469), Expect = 3.361e-55
Identity = 84/113 (74.34%), Postives = 95/113 (84.07%), Query Frame = -1 
Query:    4 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 339
            F RKGALRQK VHEVK HKF ARFFKQPTFC HC DFIWG GKQG QCQ+C+F VH+RCHE VTF+CPGA  GP T + +N HKF+ H+Y++PTFCDHCGSLLYGL HQG+KC
Sbjct:   19 FCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKC 131

HSP 2 Score: 46.595 bits (109), Expect = 5.889e-6
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = -1 
Query:  127 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGAD 288
            HKF    +  PTFC HC   ++G   QG +C  C   VH+RC   V   C G D
Sbjct:  101 HKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLC-GVD 153
[Close]
BLAST of SMED30016487 vs. Ensembl Celegans
Match: pkc-2 (Protein kinase C-like 2 [Source:UniProtKB/Swiss-Prot;Acc:P90980])

HSP 1 Score: 198.749 bits (504), Expect = 1.171e-59
Identity = 82/115 (71.30%), Postives = 94/115 (81.74%), Query Frame = -1 
Query:    4 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 345
            K F R+GALRQKNVHE+K HKF+ARFFKQPTFC HCKDF+WG  KQGFQCQ+C   VHKRCHE V F CPGAD G DT + +  HK+K  TY++PTFCDHCGSLLYG+ HQG+KC
Sbjct:  160 KAFVRRGALRQKNVHEIKSHKFIARFFKQPTFCSHCKDFLWGITKQGFQCQVCTLVVHKRCHEFVNFACPGADKGVDTDDPRQQHKWKVQTYSSPTFCDHCGSLLYGILHQGMKC 274

HSP 2 Score: 50.447 bits (119), Expect = 8.818e-8
Identity = 25/72 (34.72%), Postives = 37/72 (51.39%), Query Frame = -1 
Query:   79 KDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPH 294
            + HK+  + +  PTFC HC   ++G   QG +CQ C   VH RC + V   C G D   +TE +   + + H
Sbjct:  242 QQHKWKVQTYSSPTFCDHCGSLLYGILHQGMKCQSCDTNVHHRCVKNVPNMC-GTD---NTEKRGRLRIEAH 309
[Close]
BLAST of SMED30016487 vs. Ensembl Celegans
Match: pkc-2 (Protein kinase C-like 2 [Source:UniProtKB/Swiss-Prot;Acc:P90980])

HSP 1 Score: 187.193 bits (474), Expect = 2.047e-56
Identity = 82/115 (71.30%), Postives = 94/115 (81.74%), Query Frame = -1 
Query:    4 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEY-QNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 345
            K F R+GALRQKNVHE+K HKF+ARFFKQPTFC HCKDF+WG  KQGFQCQ+C   VHKRCHE V F CPGAD G DT+  +  HK+K  TY++PTFCDHCGSLLYG+ HQG+KC
Sbjct:   20 KAFVRRGALRQKNVHEIKSHKFIARFFKQPTFCSHCKDFLWGITKQGFQCQVCTLVVHKRCHEFVNFACPGADKGVDTDDPRQQHKWKVQTYSSPTFCDHCGSLLYGILHQGMKC 134

HSP 2 Score: 46.2098 bits (108), Expect = 3.122e-6
Identity = 25/70 (35.71%), Postives = 36/70 (51.43%), Query Frame = -1 
Query:   79 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPH 288
            HK+  + +  PTFC HC   ++G   QG +CQ C   VH RC + V   C G D   +TE +   + + H
Sbjct:  104 HKWKVQTYSSPTFCDHCGSLLYGILHQGMKCQSCDTNVHHRCVKNVPNMC-GTD---NTEKRGRLRIEAH 169
[Close]
BLAST of SMED30016487 vs. Ensembl Celegans
Match: pkc-2 (Protein kinase C-like 2 [Source:UniProtKB/Swiss-Prot;Acc:P90980])

HSP 1 Score: 187.193 bits (474), Expect = 2.300e-56
Identity = 82/115 (71.30%), Postives = 94/115 (81.74%), Query Frame = -1 
Query:    4 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 345
            K F R+GALRQKNVHE+K HKF+ARFFKQPTFC HCKDF+WG  KQGFQCQ+C   VHKRCHE V F CPGAD G DT + +  HK+K  TY++PTFCDHCGSLLYG+ HQG+KC
Sbjct:   20 KAFVRRGALRQKNVHEIKSHKFIARFFKQPTFCSHCKDFLWGITKQGFQCQVCTLVVHKRCHEFVNFACPGADKGVDTDDPRQQHKWKVQTYSSPTFCDHCGSLLYGILHQGMKC 134

HSP 2 Score: 45.8246 bits (107), Expect = 3.338e-6
Identity = 25/70 (35.71%), Postives = 36/70 (51.43%), Query Frame = -1 
Query:   79 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPH 288
            HK+  + +  PTFC HC   ++G   QG +CQ C   VH RC + V   C G D   +TE +   + + H
Sbjct:  104 HKWKVQTYSSPTFCDHCGSLLYGILHQGMKCQSCDTNVHHRCVKNVPNMC-GTD---NTEKRGRLRIEAH 169
[Close]
BLAST of SMED30016487 vs. Ensembl Celegans
Match: pkc-2 (Protein kinase C-like 2 [Source:UniProtKB/Swiss-Prot;Acc:P90980])

HSP 1 Score: 187.578 bits (475), Expect = 2.487e-56
Identity = 82/115 (71.30%), Postives = 94/115 (81.74%), Query Frame = -1 
Query:    4 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 345
            K F R+GALRQKNVHE+K HKF+ARFFKQPTFC HCKDF+WG  KQGFQCQ+C   VHKRCHE V F CPGAD G DT + +  HK+K  TY++PTFCDHCGSLLYG+ HQG+KC
Sbjct:   65 KAFVRRGALRQKNVHEIKSHKFIARFFKQPTFCSHCKDFLWGITKQGFQCQVCTLVVHKRCHEFVNFACPGADKGVDTDDPRQQHKWKVQTYSSPTFCDHCGSLLYGILHQGMKC 179

HSP 2 Score: 46.2098 bits (108), Expect = 2.919e-6
Identity = 25/70 (35.71%), Postives = 36/70 (51.43%), Query Frame = -1 
Query:   79 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPH 288
            HK+  + +  PTFC HC   ++G   QG +CQ C   VH RC + V   C G D   +TE +   + + H
Sbjct:  149 HKWKVQTYSSPTFCDHCGSLLYGILHQGMKCQSCDTNVHHRCVKNVPNMC-GTD---NTEKRGRLRIEAH 214
[Close]
BLAST of SMED30016487 vs. Ensembl Celegans
Match: pkc-1 (Protein kinase C-like 1B [Source:UniProtKB/Swiss-Prot;Acc:P34885])

HSP 1 Score: 146.747 bits (369), Expect = 1.162e-41
Identity = 70/131 (53.44%), Postives = 88/131 (67.18%), Query Frame = -1 
Query:    4 NSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPG--ADTGPDT--EYQNN----------HKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 354
            N+     R+GA+R+K +HEV  HKF+A F +QPTFC HCK+FIWG GKQG+QCQIC   VHKRCHE V ++CPG  AD   +   E Q            H+F  H+Y  PTFCDHCGS+LYGL +QG++C
Sbjct:   63 NAFNDRQRRGAMRRK-IHEVTGHKFMALFLRQPTFCAHCKEFIWGIGKQGYQCQICTVVVHKRCHEDVVWKCPGNKADAVEELGKEIQETGAGRFNINMPHRFSVHSYKRPTFCDHCGSMLYGLINQGLQC 192

HSP 2 Score: 53.9138 bits (128), Expect = 6.486e-9
Identity = 22/50 (44.00%), Postives = 27/50 (54.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            H+F    +K+PTFC HC   ++G   QG QC  C   VHKRC   V   C
Sbjct:  162 HRFSVHSYKRPTFCDHCGSMLYGLINQGLQCSTCKLNVHKRCQRNVANNC 211
[Close]
BLAST of SMED30016487 vs. Ensembl Fly
Match: Pkc53E (gene:FBgn0003091 transcript:FBtr0331960)

HSP 1 Score: 184.496 bits (467), Expect = 2.278e-55
Identity = 83/124 (66.94%), Postives = 99/124 (79.84%), Query Frame = -1 
Query:    4 GDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEY-QNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 372
            G+   +N +K   RKGAL++KNV  VKDH F+ARFFKQPTFC HCKDFIWGFGKQGFQCQ+C++ VHKRCHE VTF CPG D G D++  +  H F+P TY  PTFCDHCGSLLYG++HQG+KC
Sbjct:   18 GEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFIWGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKC 141
[Close]
BLAST of SMED30016487 vs. Ensembl Fly
Match: Pkc53E (gene:FBgn0003091 transcript:FBtr0087049)

HSP 1 Score: 184.496 bits (467), Expect = 2.278e-55
Identity = 83/124 (66.94%), Postives = 99/124 (79.84%), Query Frame = -1 
Query:    4 GDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEY-QNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 372
            G+   +N +K   RKGAL++KNV  VKDH F+ARFFKQPTFC HCKDFIWGFGKQGFQCQ+C++ VHKRCHE VTF CPG D G D++  +  H F+P TY  PTFCDHCGSLLYG++HQG+KC
Sbjct:   18 GEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFIWGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKC 141
[Close]
BLAST of SMED30016487 vs. Ensembl Fly
Match: Pkc53E (gene:FBgn0003091 transcript:FBtr0087048)

HSP 1 Score: 172.94 bits (437), Expect = 5.362e-51
Identity = 83/133 (62.41%), Postives = 99/133 (74.44%), Query Frame = -1 
Query:    4 GDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFI--------W-GFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEY-QNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 372
            G+   +N +K   RKGAL++KNV  VKDH F+ARFFKQPTFC HCKDFI        W GFGKQGFQCQ+C++ VHKRCHE VTF CPG D G D++  +  H F+P TY  PTFCDHCGSLLYG++HQG+KC
Sbjct:   18 GEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKC 150
[Close]
BLAST of SMED30016487 vs. Ensembl Fly
Match: Pkc53E (gene:FBgn0003091 transcript:FBtr0112819)

HSP 1 Score: 167.933 bits (424), Expect = 4.411e-49
Identity = 80/132 (60.61%), Postives = 99/132 (75.00%), Query Frame = -1 
Query:    4 GDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFI--------W-GFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 372
            G+   +N +K   RKGAL++KNV  VKDH F+ARFFKQPTFC HCKDFI        W GFGKQGFQCQ+C++ VHKRCHE VTF CPG D G D+ +Q  HK++  T+ +PTFCDHCGSLL+G+ H+G+KC
Sbjct:   18 GEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSAHQMPHKWQNVTFLSPTFCDHCGSLLHGITHKGLKC 149
[Close]
BLAST of SMED30016487 vs. Ensembl Fly
Match: Pkc53E (gene:FBgn0003091 transcript:FBtr0305070)

HSP 1 Score: 167.933 bits (424), Expect = 4.411e-49
Identity = 80/132 (60.61%), Postives = 99/132 (75.00%), Query Frame = -1 
Query:    4 GDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFI--------W-GFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 372
            G+   +N +K   RKGAL++KNV  VKDH F+ARFFKQPTFC HCKDFI        W GFGKQGFQCQ+C++ VHKRCHE VTF CPG D G D+ +Q  HK++  T+ +PTFCDHCGSLL+G+ H+G+KC
Sbjct:   18 GEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSAHQMPHKWQNVTFLSPTFCDHCGSLLHGITHKGLKC 149
[Close]
BLAST of SMED30016487 vs. Ensembl Zebrafish
Match: prkcba (protein kinase C, beta a [Source:ZFIN;Acc:ZDB-GENE-040426-1354])

HSP 1 Score: 196.052 bits (497), Expect = 1.195e-64
Identity = 91/128 (71.09%), Postives = 104/128 (81.25%), Query Frame = -1 
Query:    4 MSEYGDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 384
            M+E  D+      +GFAR+GALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE V F CPG+D GP + + +  H FK HTYT+PTFCDHCGSLLYGL HQG++C
Sbjct:    1 MTESSDSDGKEGARGFARQGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVHFSCPGSDKGPASDDPRKYHSFKVHTYTSPTFCDHCGSLLYGLIHQGMRC 128
[Close]
BLAST of SMED30016487 vs. Ensembl Zebrafish
Match: prkcaa (protein kinase C, alpha, a [Source:ZFIN;Acc:ZDB-GENE-050208-100])

HSP 1 Score: 204.142 bits (518), Expect = 8.043e-63
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEY-QNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHELVTF CPGAD GPDT+  ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   18 FARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHELVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 131
[Close]
BLAST of SMED30016487 vs. Ensembl Zebrafish
Match: prkcaa (protein kinase C, alpha, a [Source:ZFIN;Acc:ZDB-GENE-050208-100])

HSP 1 Score: 203.756 bits (517), Expect = 2.005e-62
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHELVTF CPGAD GPDT + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   18 FARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHELVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 131
[Close]
BLAST of SMED30016487 vs. Ensembl Zebrafish
Match: prkcbb (protein kinase C, beta b [Source:NCBI gene;Acc:393953])

HSP 1 Score: 202.216 bits (513), Expect = 6.867e-62
Identity = 90/117 (76.92%), Postives = 104/117 (88.89%), Query Frame = -1 
Query:    1 LKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 348
            ++GFAR+GALRQKNVHEVK+HKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG++CD
Sbjct:   16 MRGFARQGALRQKNVHEVKNHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCD 132

HSP 2 Score: 45.4394 bits (106), Expect = 8.056e-6
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   +HKRC   V   C
Sbjct:  101 HKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCDHCMMNIHKRCVANVPSLC 150
[Close]
BLAST of SMED30016487 vs. Ensembl Zebrafish
Match: prkcbb (protein kinase C, beta b [Source:NCBI gene;Acc:393953])

HSP 1 Score: 202.216 bits (513), Expect = 8.200e-62
Identity = 90/117 (76.92%), Postives = 104/117 (88.89%), Query Frame = -1 
Query:    1 LKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 348
            ++GFAR+GALRQKNVHEVK+HKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG++CD
Sbjct:   16 MRGFARQGALRQKNVHEVKNHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCD 132

HSP 2 Score: 45.4394 bits (106), Expect = 8.611e-6
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   +HKRC   V   C
Sbjct:  101 HKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCDHCMMNIHKRCVANVPSLC 150
[Close]
BLAST of SMED30016487 vs. Ensembl Xenopus
Match: prkcb (protein kinase C beta [Source:NCBI gene;Acc:100038043])

HSP 1 Score: 198.364 bits (503), Expect = 2.594e-60
Identity = 92/123 (74.80%), Postives = 105/123 (85.37%), Query Frame = -1 
Query:    1 TFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 366
            T +D + + FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KC+
Sbjct:    5 TDIDTTTR-FARKGALRQKNVHEVKEHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCE 126

HSP 2 Score: 47.7506 bits (112), Expect = 1.739e-6
Identity = 21/50 (42.00%), Postives = 26/50 (52.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C+ C   VHKRC   V   C
Sbjct:   95 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCETCMMNVHKRCVMNVPSLC 144
[Close]
BLAST of SMED30016487 vs. Ensembl Xenopus
Match: prkcb (protein kinase C beta [Source:NCBI gene;Acc:100038043])

HSP 1 Score: 198.364 bits (503), Expect = 3.536e-60
Identity = 91/121 (75.21%), Postives = 103/121 (85.12%), Query Frame = -1 
Query:    1 VDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 360
            V ++   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KC+
Sbjct:   11 VADTTTRFARKGALRQKNVHEVKEHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCE 131

HSP 2 Score: 47.3654 bits (111), Expect = 2.407e-6
Identity = 21/50 (42.00%), Postives = 26/50 (52.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C+ C   VHKRC   V   C
Sbjct:  100 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCETCMMNVHKRCVMNVPSLC 149
[Close]
BLAST of SMED30016487 vs. Ensembl Xenopus
Match: prkcb (protein kinase C beta [Source:NCBI gene;Acc:100038043])

HSP 1 Score: 197.208 bits (500), Expect = 4.786e-60
Identity = 90/120 (75.00%), Postives = 103/120 (85.83%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            +++   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KC+
Sbjct:   11 EDTTTRFARKGALRQKNVHEVKEHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCE 130

HSP 2 Score: 47.3654 bits (111), Expect = 2.319e-6
Identity = 21/50 (42.00%), Postives = 26/50 (52.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C+ C   VHKRC   V   C
Sbjct:   99 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCETCMMNVHKRCVMNVPSLC 148
[Close]
BLAST of SMED30016487 vs. Ensembl Xenopus
Match: prkcb (protein kinase C beta [Source:NCBI gene;Acc:100038043])

HSP 1 Score: 197.593 bits (501), Expect = 6.052e-60
Identity = 90/120 (75.00%), Postives = 103/120 (85.83%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            +++   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KC+
Sbjct:   11 EDTTTRFARKGALRQKNVHEVKEHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCE 130

HSP 2 Score: 47.7506 bits (112), Expect = 2.188e-6
Identity = 21/50 (42.00%), Postives = 26/50 (52.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C+ C   VHKRC   V   C
Sbjct:   99 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCETCMMNVHKRCVMNVPSLC 148
[Close]
BLAST of SMED30016487 vs. Ensembl Xenopus
Match: prkcb (protein kinase C beta [Source:NCBI gene;Acc:100038043])

HSP 1 Score: 197.593 bits (501), Expect = 6.927e-60
Identity = 90/120 (75.00%), Postives = 103/120 (85.83%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            +++   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KC+
Sbjct:    8 NDTTTRFARKGALRQKNVHEVKEHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCE 127

HSP 2 Score: 47.3654 bits (111), Expect = 2.271e-6
Identity = 21/50 (42.00%), Postives = 26/50 (52.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C+ C   VHKRC   V   C
Sbjct:   96 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCETCMMNVHKRCVMNVPSLC 145
[Close]
BLAST of SMED30016487 vs. Ensembl Mouse
Match: Prkca (protein kinase C, alpha [Source:MGI Symbol;Acc:MGI:97595])

HSP 1 Score: 204.912 bits (520), Expect = 8.713e-63
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GPDT + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   20 FARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 133
[Close]
BLAST of SMED30016487 vs. Ensembl Mouse
Match: Prkcb (protein kinase C, beta [Source:MGI Symbol;Acc:MGI:97596])

HSP 1 Score: 200.29 bits (508), Expect = 5.446e-61
Identity = 92/120 (76.67%), Postives = 102/120 (85.00%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            + S   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KCD
Sbjct:   14 EESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCD 133

HSP 2 Score: 46.9802 bits (110), Expect = 2.881e-6
Identity = 21/50 (42.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   VHKRC   V   C
Sbjct:  102 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLC 151
[Close]
BLAST of SMED30016487 vs. Ensembl Mouse
Match: Prkcb (protein kinase C, beta [Source:MGI Symbol;Acc:MGI:97596])

HSP 1 Score: 199.904 bits (507), Expect = 6.758e-61
Identity = 92/120 (76.67%), Postives = 102/120 (85.00%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            + S   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KCD
Sbjct:   14 EESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCD 133

HSP 2 Score: 46.9802 bits (110), Expect = 3.052e-6
Identity = 21/50 (42.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   VHKRC   V   C
Sbjct:  102 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLC 151
[Close]
BLAST of SMED30016487 vs. Ensembl Mouse
Match: Prkcb (protein kinase C, beta [Source:MGI Symbol;Acc:MGI:97596])

HSP 1 Score: 199.904 bits (507), Expect = 6.758e-61
Identity = 92/120 (76.67%), Postives = 102/120 (85.00%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            + S   FARKGALRQKNVHEVK+HKF ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KCD
Sbjct:   14 EESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCD 133

HSP 2 Score: 46.9802 bits (110), Expect = 3.052e-6
Identity = 21/50 (42.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   VHKRC   V   C
Sbjct:  102 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLC 151
[Close]
BLAST of SMED30016487 vs. Ensembl Mouse
Match: Prkcg (Protein kinase C gamma type [Source:UniProtKB/Swiss-Prot;Acc:P63318])

HSP 1 Score: 186.037 bits (471), Expect = 7.956e-56
Identity = 84/113 (74.34%), Postives = 95/113 (84.07%), Query Frame = -1 
Query:    4 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 339
            F RKGALRQK VHEVK HKF ARFFKQPTFC HC DFIWG GKQG QCQ+C+F VH+RCHE VTF+CPGA  GP T + +N HKF+ H+Y++PTFCDHCGSLLYGL HQG+KC
Sbjct:   19 FCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKC 131

HSP 2 Score: 46.595 bits (109), Expect = 3.368e-6
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = -1 
Query:  127 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGAD 288
            HKF    +  PTFC HC   ++G   QG +C  C   VH+RC   V   C G D
Sbjct:  101 HKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLC-GVD 153
[Close]
BLAST of SMED30016487 vs. UniProt/SwissProt
Match: sp|P05696|KPCA_RAT (Protein kinase C alpha type OS=Rattus norvegicus OX=10116 GN=Prkca PE=1 SV=3)

HSP 1 Score: 204.912 bits (520), Expect = 6.031e-62
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GPDT + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   20 FARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 133
[Close]
BLAST of SMED30016487 vs. UniProt/SwissProt
Match: sp|P20444|KPCA_MOUSE (Protein kinase C alpha type OS=Mus musculus OX=10090 GN=Prkca PE=1 SV=3)

HSP 1 Score: 204.912 bits (520), Expect = 6.095e-62
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GPDT + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   20 FARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 133
[Close]
BLAST of SMED30016487 vs. UniProt/SwissProt
Match: sp|P17252|KPCA_HUMAN (Protein kinase C alpha type OS=Homo sapiens OX=9606 GN=PRKCA PE=1 SV=4)

HSP 1 Score: 204.912 bits (520), Expect = 6.223e-62
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GPDT + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   20 FARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 133
[Close]
BLAST of SMED30016487 vs. UniProt/SwissProt
Match: sp|P10102|KPCA_RABIT (Protein kinase C alpha type OS=Oryctolagus cuniculus OX=9986 GN=PRKCA PE=2 SV=3)

HSP 1 Score: 204.912 bits (520), Expect = 6.289e-62
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GPDT + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   20 FARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCD 133
[Close]
BLAST of SMED30016487 vs. UniProt/SwissProt
Match: sp|Q16974|KPC1_APLCA (Calcium-dependent protein kinase C OS=Aplysia californica OX=6500 GN=PRKC1 PE=1 SV=2)

HSP 1 Score: 204.142 bits (518), Expect = 9.408e-62
Identity = 91/115 (79.13%), Postives = 100/115 (86.96%), Query Frame = -1 
Query:    1 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 345
            K  AR+GALRQKNVHEVK+HKFLARFFKQPTFC HCKDFIWGFGKQGFQCQ+C+  VHKRCHE V F CPGAD GPD++  N HKFK H+Y +PTFCDHCGSLLYGL HQG+KCD
Sbjct:    3 KRVARRGALRQKNVHEVKNHKFLARFFKQPTFCSHCKDFIWGFGKQGFQCQVCSLVVHKRCHEFVCFICPGADKGPDSDATNLHKFKLHSYGSPTFCDHCGSLLYGLLHQGLKCD 117

HSP 2 Score: 48.521 bits (114), Expect = 5.915e-6
Identity = 23/63 (36.51%), Postives = 30/63 (47.62%), Query Frame = -1 
Query:  139 GALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 327
            GA +  +      HKF    +  PTFC HC   ++G   QG +C  C   VHKRC + V   C
Sbjct:   73 GADKGPDSDATNLHKFKLHSYGSPTFCDHCGSLLYGLLHQGLKCDSCDMNVHKRCEKNVPLLC 135
[Close]
BLAST of SMED30016487 vs. TrEMBL
Match: A0A1I8IRL8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 2.570e-71
Identity = 99/126 (78.57%), Postives = 113/126 (89.68%), Query Frame = -1 
Query:    1 EYGDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 378
            E  D  V++ +K  ARKGALRQKN HE+KDHKF+ARFFKQPTFCGHC DFIWGFGKQGFQC+IC+FAVHKRCHELVTF CPGAD GPDT++QN+HKFK  +YT+PTFCDHCGSLLYGLFHQG+KC+
Sbjct: 1665 ENEDNEVEHQVKVVARKGALRQKNEHEIKDHKFVARFFKQPTFCGHCTDFIWGFGKQGFQCKICSFAVHKRCHELVTFACPGADKGPDTDFQNDHKFKVTSYTSPTFCDHCGSLLYGLFHQGLKCE 1790

HSP 2 Score: 58.9214 bits (141), Expect = 4.518e-7
Identity = 29/76 (38.16%), Postives = 37/76 (48.68%), Query Frame = -1 
Query:  127 NSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGAD 354
            + L  FA  GA +  +     DHKF    +  PTFC HC   ++G   QG +C+ C   VHKRC   V   C G D
Sbjct: 1737 HELVTFACPGADKGPDTDFQNDHKFKVTSYTSPTFCDHCGSLLYGLFHQGLKCESCDINVHKRCQNNVPKLC-GVD 1811
[Close]
BLAST of SMED30016487 vs. TrEMBL
Match: A0A1I8H4R2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 7.268e-68
Identity = 96/115 (83.48%), Postives = 110/115 (95.65%), Query Frame = -1 
Query:    1 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 345
            KGFAR+GALRQKNVHEVK+HKF+ARFFKQPTFCGHC DFIWGFGKQGFQC+IC+FAVHKRCHELVTF CPGAD GPD++YQN+H+F+  +YT+PTFCDHCGSLLYGLFHQG+KC+
Sbjct:   12 KGFARRGALRQKNVHEVKNHKFVARFFKQPTFCGHCTDFIWGFGKQGFQCKICSFAVHKRCHELVTFACPGADRGPDSDYQNSHRFRVISYTSPTFCDHCGSLLYGLFHQGLKCE 126
[Close]
BLAST of SMED30016487 vs. TrEMBL
Match: A0A267EY79 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027843g1 PE=4 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 2.924e-66
Identity = 96/115 (83.48%), Postives = 110/115 (95.65%), Query Frame = -1 
Query:    1 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 345
            KGFAR+GALRQKNVHEVK+HKF+ARFFKQPTFCGHC DFIWGFGKQGFQC+IC+FAVHKRCHELVTF CPGAD GPD++YQN+H+F+  +YT+PTFCDHCGSLLYGLFHQG+KC+
Sbjct:   12 KGFARRGALRQKNVHEVKNHKFVARFFKQPTFCGHCTDFIWGFGKQGFQCKICSFAVHKRCHELVTFACPGADRGPDSDYQNSHRFRVISYTSPTFCDHCGSLLYGLFHQGLKCE 126
[Close]
BLAST of SMED30016487 vs. TrEMBL
Match: A0A267GSY1 (Protein kinase C OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032341g1 PE=3 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 7.924e-66
Identity = 99/126 (78.57%), Postives = 113/126 (89.68%), Query Frame = -1 
Query:    1 EYGDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 378
            E  D  V++ +K  ARKGALRQKN HE+KDHKF+ARFFKQPTFCGHC DFIWGFGKQGFQC+IC+FAVHKRCHELVTF CPGAD GPDT++QN+HKFK  +YT+PTFCDHCGSLLYGLFHQG+KC+
Sbjct:    2 ENEDNEVEHQVKVVARKGALRQKNEHEIKDHKFVARFFKQPTFCGHCTDFIWGFGKQGFQCKICSFAVHKRCHELVTFACPGADKGPDTDFQNDHKFKVTSYTSPTFCDHCGSLLYGLFHQGLKCE 127
[Close]
BLAST of SMED30016487 vs. TrEMBL
Match: C3YC98 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_88030 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.206e-65
Identity = 97/120 (80.83%), Postives = 107/120 (89.17%), Query Frame = -1 
Query:    1 DNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 357
            D   +GFARKGALRQKNVHEVKDHKF+ARFFKQPTFC HCKDFIWGFGKQGFQCQ+C+F VHKRCHE VTFQCPGAD GPD+ + ++ HKFK HTY +PTFCDHCGSLLYGL HQG+KCD
Sbjct:    6 DTQPRGFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCKDFIWGFGKQGFQCQVCSFVVHKRCHEYVTFQCPGADAGPDSDDPRSKHKFKVHTYGSPTFCDHCGSLLYGLLHQGMKCD 125
[Close]
BLAST of SMED30016487 vs. Ensembl Cavefish
Match: prkcba (protein kinase C beta [Source:NCBI gene;Acc:103036012])

HSP 1 Score: 206.838 bits (525), Expect = 1.336e-63
Identity = 94/125 (75.20%), Postives = 106/125 (84.80%), Query Frame = -1 
Query:    1 GDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 372
            GD   +   +GFAR+GALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KCD
Sbjct:    8 GDAESEGGARGFARQGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSPTFCDHCGSLLYGLIHQGMKCD 132

HSP 2 Score: 46.595 bits (109), Expect = 2.737e-6
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   +HKRC   V   C
Sbjct:  101 HKFKVHTYSSPTFCDHCGSLLYGLIHQGMKCDNCMMNIHKRCVTNVPSLC 150
[Close]
BLAST of SMED30016487 vs. Ensembl Cavefish
Match: prkcab (protein kinase C, alpha, b [Source:ZFIN;Acc:ZDB-GENE-131121-412])

HSP 1 Score: 201.06 bits (510), Expect = 4.775e-62
Identity = 91/114 (79.82%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVK+HKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE V+F CPGAD GPDT + ++ HKFK H+Y +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   18 FARKGALRQKNVHEVKNHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVSFSCPGADKGPDTDDPRSKHKFKIHSYGSPTFCDHCGSLLYGLIHQGMKCD 131
[Close]
BLAST of SMED30016487 vs. Ensembl Cavefish
Match: prkcbb (protein kinase C beta type [Source:NCBI gene;Acc:103025973])

HSP 1 Score: 201.445 bits (511), Expect = 1.023e-61
Identity = 90/116 (77.59%), Postives = 103/116 (88.79%), Query Frame = -1 
Query:    1 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEY-QNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 345
            +GFAR+GALRQKNVHEVK+HKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP ++  ++ HKFK HTY++PTFCDHCGSLLYGL HQG++CD
Sbjct:   16 RGFARQGALRQKNVHEVKNHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCD 131

HSP 2 Score: 45.4394 bits (106), Expect = 6.541e-6
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   +HKRC   V   C
Sbjct:  100 HKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCDHCMMNIHKRCVANVPSLC 149
[Close]
BLAST of SMED30016487 vs. Ensembl Cavefish
Match: prkcbb (protein kinase C beta type [Source:NCBI gene;Acc:103025973])

HSP 1 Score: 201.06 bits (510), Expect = 2.213e-61
Identity = 90/116 (77.59%), Postives = 103/116 (88.79%), Query Frame = -1 
Query:    1 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 345
            +GFAR+GALRQKNVHEVK+HKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG++CD
Sbjct:   16 RGFARQGALRQKNVHEVKNHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCD 131

HSP 2 Score: 45.4394 bits (106), Expect = 7.030e-6
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   +HKRC   V   C
Sbjct:  100 HKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCDHCMMNIHKRCVANVPSLC 149
[Close]
BLAST of SMED30016487 vs. Ensembl Cavefish
Match: prkcg (protein kinase C, gamma [Source:ZFIN;Acc:ZDB-GENE-090206-1])

HSP 1 Score: 201.06 bits (510), Expect = 4.373e-60
Identity = 84/113 (74.34%), Postives = 94/113 (83.19%), Query Frame = -1 
Query:    4 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTE-YQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 339
            F RKGAL+ K + EVKDH+F ARFFKQPTFC HC DFIWG GKQGFQCQ+C+F VHKRCHE VTF CPGA TGP  E  ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KC
Sbjct:  627 FQRKGALKHKRIVEVKDHQFTARFFKQPTFCSHCTDFIWGIGKQGFQCQVCSFVVHKRCHEFVTFTCPGAITGPKAEDLKSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKC 739

HSP 2 Score: 53.5286 bits (127), Expect = 1.161e-8
Identity = 25/68 (36.76%), Postives = 32/68 (47.06%), Query Frame = -1 
Query:  139 FARKGALRQKNVHEVKD-HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 339
            F   GA+      ++K  HKF    +  PTFC HC   ++G   QG +C  C   VHKRC   V   C
Sbjct:  691 FTCPGAITGPKAEDLKSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCASCDMNVHKRCESSVPSLC 758
[Close]
BLAST of SMED30016487 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002458.1 (pep scaffold:Pmarinus_7.0:GL479588:3:23579:-1 gene:ENSPMAG00000002237.1 transcript:ENSPMAT00000002458.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 124.79 bits (312), Expect = 1.820e-36
Identity = 57/119 (47.90%), Postives = 80/119 (67.23%), Query Frame = -1 
Query:    1 RKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPG-ADTGPDTEYQNN-------HKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 333
            R+GA++Q  VH VK H+F A FF QPTFC  CK++  G  KQG+QC++C  A+HKRC ++V   C G A+   +T+           H+F+ ++Y +PTFCDHCG+LL+GL  QG+KCD
Sbjct:  103 RRGAIKQAKVHHVKGHQFTATFFPQPTFCSVCKEYFKGLNKQGYQCRVCNAAIHKRCIDIVLANCTGSAENSSETQIMKERFKLDVPHRFRVNSYMSPTFCDHCGTLLWGLARQGLKCD 221

HSP 2 Score: 51.2174 bits (121), Expect = 1.073e-8
Identity = 19/50 (38.00%), Postives = 26/50 (52.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            H+F    +  PTFC HC   +WG  +QG +C  CA  +H +C   V   C
Sbjct:  190 HRFRVNSYMSPTFCDHCGTLLWGLARQGLKCDECAMNIHHKCKGKVANMC 239
[Close]
BLAST of SMED30016487 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002460.1 (pep scaffold:Pmarinus_7.0:GL479588:3:23579:-1 gene:ENSPMAG00000002237.1 transcript:ENSPMAT00000002460.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 124.405 bits (311), Expect = 1.936e-36
Identity = 57/119 (47.90%), Postives = 80/119 (67.23%), Query Frame = -1 
Query:    1 RKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPG-ADTGPDTEYQNN-------HKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 333
            R+GA++Q  VH VK H+F A FF QPTFC  CK++  G  KQG+QC++C  A+HKRC ++V   C G A+   +T+           H+F+ ++Y +PTFCDHCG+LL+GL  QG+KCD
Sbjct:  102 RRGAIKQAKVHHVKGHQFTATFFPQPTFCSVCKEYFKGLNKQGYQCRVCNAAIHKRCIDIVLANCTGSAENSSETQIMKERFKLDVPHRFRVNSYMSPTFCDHCGTLLWGLARQGLKCD 220

HSP 2 Score: 51.2174 bits (121), Expect = 1.088e-8
Identity = 19/50 (38.00%), Postives = 26/50 (52.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            H+F    +  PTFC HC   +WG  +QG +C  CA  +H +C   V   C
Sbjct:  189 HRFRVNSYMSPTFCDHCGTLLWGLARQGLKCDECAMNIHHKCKGKVANMC 238
[Close]
BLAST of SMED30016487 vs. Ensembl Sea Lamprey
Match: PRKCD (protein kinase C delta [Source:HGNC Symbol;Acc:HGNC:9399])

HSP 1 Score: 120.553 bits (301), Expect = 5.092e-33
Identity = 59/119 (49.58%), Postives = 77/119 (64.71%), Query Frame = -1 
Query:    1 RKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPG-ADTGPDTEYQN-------NHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 333
            R+GA++Q  V  VK H F+  FF QPTFC  CK+F+WG  KQGFQC+ C  + HKRC + V  +C G ADT   TE          +H FK H+Y +PTFCDHCG+LL+G   QG++C+
Sbjct:    2 RRGAIKQAKVKFVKGHAFVTTFFPQPTFCSVCKEFVWGLNKQGFQCKECNASSHKRCVDNVLARCTGSADTNRQTEIVRESFNLHMSHSFKVHSYRSPTFCDHCGTLLWGFARQGLQCE 120

HSP 2 Score: 51.6026 bits (122), Expect = 1.023e-8
Identity = 23/57 (40.35%), Postives = 30/57 (52.63%), Query Frame = -1 
Query:  139 NVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 309
            N+H    H F    ++ PTFC HC   +WGF +QG QC+ C   VH +C   V   C
Sbjct:   84 NLH--MSHSFKVHSYRSPTFCDHCGTLLWGFARQGLQCEACDANVHHKCKGKVANLC 138
[Close]
BLAST of SMED30016487 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000354.1 (pep scaffold:Pmarinus_7.0:GL488232:2313:7448:1 gene:ENSPMAG00000000317.1 transcript:ENSPMAT00000000354.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 71.2478 bits (173), Expect = 1.479e-17
Identity = 30/65 (46.15%), Postives = 42/65 (64.62%), Query Frame = -1 
Query:  139 RKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 333
            R+GA R + ++ V  H F A+ F +  FC +C D IWG G+QG++C  C   VHKRCH+LV  +C
Sbjct:    5 RRGARRWRKLYLVNGHTFQAKRFNRRAFCAYCTDRIWGLGRQGYKCINCKLLVHKRCHKLVAVEC 69
[Close]
BLAST of SMED30016487 vs. Ensembl Sea Lamprey
Match: PRKCE (protein kinase C epsilon [Source:HGNC Symbol;Acc:HGNC:9401])

HSP 1 Score: 76.6406 bits (187), Expect = 1.487e-17
Identity = 34/63 (53.97%), Postives = 41/63 (65.08%), Query Frame = -1 
Query:    4 AVHKRCHELVTFQCPGADTGPDTEYQNN----HKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 180
             VHKRCHEL+  +CPG       EY ++    HKF  H Y  PTFCDHCGSLL+GL  QG++C
Sbjct:    2 VVHKRCHELIITKCPGMKKEAQDEYPSSVNVPHKFSIHNYKVPTFCDHCGSLLWGLLRQGLQC 64

HSP 2 Score: 59.6918 bits (143), Expect = 1.698e-11
Identity = 35/100 (35.00%), Postives = 45/100 (45.00%), Query Frame = -1 
Query:   28 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGAD------------TGPDTEYQNN-HKFKPHTYTTPTFCDHCGSLLYG 288
            HKF    +K PTFC HC   +WG  +QG QC++C   VH+RC + V   C G D              PD  +Q+   K KP  Y          SL+ G
Sbjct:   34 HKFSIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCEQNVAPSC-GVDARGIAKVLAEIGVTPDKIHQSTIKKKKPAIYPRNKTAQISISLVQG 132
[Close]
BLAST of SMED30016487 vs. Ensembl Yeast
Match: PKC1 (Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) [Source:SGD;Acc:S000000201])

HSP 1 Score: 69.3218 bits (168), Expect = 5.091e-15
Identity = 41/123 (33.33%), Postives = 59/123 (47.97%), Query Frame = -1 
Query:    4 LKGFARKGAL--RQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC---PGADTGPDTEYQNN---HKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 348
            + G  R GA+  R++ + E   H F+ + F     C +C DF+      GFQCQ C F  HK+C+  V  +C      DT PD    N+   H+F P +     +C HCG +L    H+  KC
Sbjct:  393 MGGLHRHGAIINRKEEIFEQHGHHFVQKSFYNIMCCAYCGDFL---RYTGFQCQDCKFLCHKKCYTNVVTKCIAKTSTDTDPDEAKLNHRIPHRFLPTSNRGTKWCCHCGYILPWGRHKVRKC 512
[Close]
BLAST of SMED30016487 vs. Ensembl Nematostella
Match: EDO35585 (Protein kinase C [Source:UniProtKB/TrEMBL;Acc:A7SL27])

HSP 1 Score: 122.865 bits (307), Expect = 2.805e-33
Identity = 54/112 (48.21%), Postives = 73/112 (65.18%), Query Frame = -1 
Query:    4 VHEVKDHKFLARFFKQPTFCGHCKDFIWG-FGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT----------EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 306
            +H++  HKF+A + +QPTFC HC DFIWG  GKQG+QCQ+C   VHKRCH++V  +CPG     +T          +    HKF+  +Y  PTFC+ CGSLLYG+  QG++C
Sbjct:  160 IHQINSHKFMATYLRQPTFCSHCNDFIWGVIGKQGYQCQVCTCVVHKRCHKMVVTKCPGTRQDAETMPLGGGSLRFKMDVPHKFEKKSYNRPTFCELCGSLLYGIVRQGMQC 271

HSP 2 Score: 54.299 bits (129), Expect = 2.542e-9
Identity = 21/50 (42.00%), Postives = 30/50 (60.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF  + + +PTFC  C   ++G  +QG QC +C   VH+RCH+ V   C
Sbjct:  241 HKFEKKSYNRPTFCELCGSLLYGIVRQGMQCTVCKMNVHRRCHQNVANNC 290
[Close]
BLAST of SMED30016487 vs. Ensembl Nematostella
Match: EDO44926 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUL9])

HSP 1 Score: 95.9005 bits (237), Expect = 4.315e-27
Identity = 42/66 (63.64%), Postives = 57/66 (86.36%), Query Frame = -1 
Query:  151 KGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWG-FGKQGFQCQICAFAVHKRCHELV 345
            +GF R+GAL++K+V +V+DH+F+A++FKQPTFC HCKDFIWG F  QG++C +C+F VHKRCHE V
Sbjct:    1 RGFNRRGALKKKHVFKVRDHQFIAKYFKQPTFCAHCKDFIWGLFDAQGYKCTVCSFVVHKRCHEFV 66
[Close]
BLAST of SMED30016487 vs. Ensembl Nematostella
Match: EDO44938 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RU22])

HSP 1 Score: 75.8702 bits (185), Expect = 6.605e-17
Identity = 29/80 (36.25%), Postives = 44/80 (55.00%), Query Frame = -1 
Query:  139 EYGDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 378
            +Y     +   +   R+GA R + +H V  H F+A+ F +   C  C D IWG G+ G++C  C   VHK+CH+L+  QC
Sbjct:   93 QYPGQLCNGETRNIYRRGAKRWRKIHIVNGHSFVAKRFSRLAACAFCSDRIWGLGRSGYKCLECKLVVHKKCHKLIKIQC 172
[Close]
BLAST of SMED30016487 vs. Ensembl Nematostella
Match: EDO34591 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNX6])

HSP 1 Score: 69.707 bits (169), Expect = 1.209e-14
Identity = 25/55 (45.45%), Postives = 32/55 (58.18%), Query Frame = -1 
Query:  139 HEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 303
            H    H+F+ + F +PT+C HC D +WGF  QGF C +C F  H RC   VT  C
Sbjct:   30 HGQSGHRFVRKTFTRPTYCHHCTDMLWGFTNQGFMCDVCNFVTHDRCMIFVTIPC 84

HSP 2 Score: 45.0542 bits (105), Expect = 3.243e-6
Identity = 18/46 (39.13%), Postives = 25/46 (54.35%), Query Frame = -1 
Query:    1 PGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 138
            P  D       Q+ H+F   T+T PT+C HC  +L+G  +QG  CD
Sbjct:   21 PILDEAFQAHGQSGHRFVRKTFTRPTYCHHCTDMLWGFTNQGFMCD 66
[Close]
BLAST of SMED30016487 vs. Ensembl Nematostella
Match: EDO41241 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4Z5])

HSP 1 Score: 60.4622 bits (145), Expect = 1.995e-11
Identity = 39/149 (26.17%), Postives = 51/149 (34.23%), Query Frame = -1 
Query:    4 KDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC-------------------PGAD--TGPDTEYQNN-------------------------------HKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 294
            + H+     +K PTFC  C   ++G  +QG +C  C    HKRC   +   C                   PG D  T P  +  N+                               H F  H+Y  PT C  C  LL GLF QG +C
Sbjct:   99 RSHQLAVHSYKSPTFCDFCGQMLYGLVRQGLKCDGCGGNFHKRCAYRIRNNCSDSKHRISGHFNTLPRNVAPGLDLCTPPSLQPINSWNEGGHNLRDRRATWNGDSLMWSKPGRIQVPHTFAVHSYKKPTMCQICKRLLRGLFRQGFQC 247

HSP 2 Score: 53.9138 bits (128), Expect = 3.193e-9
Identity = 20/39 (51.28%), Postives = 26/39 (66.67%), Query Frame = -1 
Query:    1 DTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 117
            D     +H+   H+Y +PTFCD CG +LYGL  QG+KCD
Sbjct:   94 DETIARSHQLAVHSYKSPTFCDFCGQMLYGLVRQGLKCD 132

HSP 3 Score: 51.9878 bits (123), Expect = 1.330e-8
Identity = 21/52 (40.38%), Postives = 28/52 (53.85%), Query Frame = -1 
Query:  133 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPG 288
            H F    +K+PT C  CK  + G  +QGFQC+ C F  H++C E     C G
Sbjct:  217 HTFAVHSYKKPTMCQICKRLLRGLFRQGFQCKDCKFNCHRKCAEKAPKNCLG 268
[Close]
BLAST of SMED30016487 vs. Ensembl Medaka
Match: prkcba (protein kinase C beta type [Source:NCBI gene;Acc:101161464])

HSP 1 Score: 203.371 bits (516), Expect = 2.493e-62
Identity = 92/119 (77.31%), Postives = 104/119 (87.39%), Query Frame = -1 
Query:    1 NSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 354
             S +GFAR+GALRQKNVHEVK+HKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KCD
Sbjct:   12 ESARGFARQGALRQKNVHEVKNHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSPTFCDHCGSLLYGLIHQGMKCD 130

HSP 2 Score: 45.8246 bits (107), Expect = 5.394e-6
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   +HKRC   V   C
Sbjct:   99 HKFKVHTYSSPTFCDHCGSLLYGLIHQGMKCDHCMMNIHKRCVANVPSLC 148
[Close]
BLAST of SMED30016487 vs. Ensembl Medaka
Match: prkcba (protein kinase C beta type [Source:NCBI gene;Acc:101161464])

HSP 1 Score: 202.986 bits (515), Expect = 3.375e-62
Identity = 92/119 (77.31%), Postives = 104/119 (87.39%), Query Frame = -1 
Query:    1 NSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 354
             S +GFAR+GALRQKNVHEVK+HKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE VTF CPGAD GP + + ++ HKFK HTY++PTFCDHCGSLLYGL HQG+KCD
Sbjct:   12 ESARGFARQGALRQKNVHEVKNHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSPTFCDHCGSLLYGLIHQGMKCD 130

HSP 2 Score: 45.8246 bits (107), Expect = 4.905e-6
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   +HKRC   V   C
Sbjct:   99 HKFKVHTYSSPTFCDHCGSLLYGLIHQGMKCDHCMMNIHKRCVANVPSLC 148
[Close]
BLAST of SMED30016487 vs. Ensembl Medaka
Match: PRKCA (protein kinase C alpha [Source:NCBI gene;Acc:101160465])

HSP 1 Score: 203.371 bits (516), Expect = 4.415e-62
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = -1 
Query:    1 FARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEY-QNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 339
            FARKGALRQKNVHEVKDHKF+ARFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHELVTF CPGAD GPDT+  +  HKFK HT+ +PTFCDHCGSLLYGL HQG+KCD
Sbjct:   18 FARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHELVTFCCPGADKGPDTDDPRTKHKFKIHTFGSPTFCDHCGSLLYGLIHQGMKCD 131
[Close]
BLAST of SMED30016487 vs. Ensembl Medaka
Match: PRKCB (protein kinase C beta [Source:NCBI gene;Acc:105353642])

HSP 1 Score: 199.519 bits (506), Expect = 6.230e-61
Identity = 89/117 (76.07%), Postives = 101/117 (86.32%), Query Frame = -1 
Query:    1 LKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 348
            ++GFAR+GALRQKNVHEVKDHKF+ RFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE V F CPGAD GP + + +  HKFK HTY++PTFCDHCGSLLYGL HQG++CD
Sbjct:   19 MRGFARQGALRQKNVHEVKDHKFIPRFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVAFSCPGADKGPASDDPRAKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMRCD 135

HSP 2 Score: 45.4394 bits (106), Expect = 7.467e-6
Identity = 20/50 (40.00%), Postives = 25/50 (50.00%), Query Frame = -1 
Query:  139 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 288
            HKF    +  PTFC HC   ++G   QG +C  C   +HKRC   V   C
Sbjct:  104 HKFKIHTYSSPTFCDHCGSLLYGLIHQGMRCDHCMMNIHKRCVANVPSLC 153
[Close]
BLAST of SMED30016487 vs. Ensembl Medaka
Match: PRKCB (protein kinase C beta [Source:NCBI gene;Acc:105353642])

HSP 1 Score: 199.134 bits (505), Expect = 1.020e-60
Identity = 89/117 (76.07%), Postives = 101/117 (86.32%), Query Frame = -1 
Query:    1 LKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDT-EYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 348
            ++GFAR+GALRQKNVHEVKDHKF+ RFFKQPTFC HC DFIWGFGKQGFQCQ+C F VHKRCHE V F CPGAD GP + + +  HKFK HTY++PTFCDHCGSLLYGL HQG++CD
Sbjct:   19 MRGFARQGALRQKNVHEVKDHKFIPRFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVAFSCPGADKGPASDDPRAKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMRCD 135
[Close]
BLAST of SMED30016487 vs. Planmine SMEST
Match: SMESG000071570.1 (SMESG000071570.1)

HSP 1 Score: 278.87 bits (712), Expect = 2.638e-91
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = -1 
Query:    1 MSEYGDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 384
            MSEYGDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD
Sbjct:    1 MSEYGDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 128
[Close]
BLAST of SMED30016487 vs. Planmine SMEST
Match: SMESG000039854.1 (SMESG000039854.1)

HSP 1 Score: 207.223 bits (526), Expect = 3.258e-69
Identity = 99/129 (76.74%), Postives = 111/129 (86.05%), Query Frame = -1 
Query:    1 MSEYGDTFVDNSLK-GFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 384
            MSE      + S K GF R+GALRQKNVHE+KDH+F+ARFFKQ TFCGHCKDFIWGFGKQGFQC+IC FAVHKRCHELVTFQCPG D GPDT+++ NH FK HTYT+PTFCDHCGSLLYGL HQG+KC+
Sbjct:    1 MSEQAIEEGNTSAKAGFTRRGALRQKNVHEIKDHQFVARFFKQFTFCGHCKDFIWGFGKQGFQCKICLFAVHKRCHELVTFQCPGKDDGPDTDFKTNHDFKVHTYTSPTFCDHCGSLLYGLLHQGLKCE 129

HSP 2 Score: 44.2838 bits (103), Expect = 7.310e-6
Identity = 19/51 (37.25%), Postives = 27/51 (52.94%), Query Frame = -1 
Query:  139 DHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQC 291
            +H F    +  PTFC HC   ++G   QG +C+ C   VHK+C + V   C
Sbjct:   97 NHDFKVHTYTSPTFCDHCGSLLYGLLHQGLKCETCDTNVHKKCEKNVPKLC 147
[Close]
BLAST of SMED30016487 vs. Planmine SMEST
Match: SMESG000042308.1 (SMESG000042308.1)

HSP 1 Score: 126.716 bits (317), Expect = 2.149e-34
Identity = 61/119 (51.26%), Postives = 78/119 (65.55%), Query Frame = -1 
Query:    1 RKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGA-DTGPDTEYQNN-------HKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 333
            R GALR++ +H++K HKF    FKQ TFC  C  FIWG   QG+QCQ+C   VHKRC++ V  +CPG  DT   +E Q N       HKF+ HT+  P FCDHCGSLL+GL  QG++C+
Sbjct:  157 RAGALRRR-IHQIKGHKFQVTLFKQFTFCSLCNSFIWGLWNQGYQCQVCTCVVHKRCYQNVIVRCPGVKDTYSSSEAQLNRFNINVPHKFRVHTFMRPAFCDHCGSLLFGLMRQGLQCE 274

HSP 2 Score: 52.7582 bits (125), Expect = 1.722e-8
Identity = 23/55 (41.82%), Postives = 31/55 (56.36%), Query Frame = -1 
Query:  124 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADT 288
            HKF    F +P FC HC   ++G  +QG QC+ C   +HKRC + V   C G +T
Sbjct:  243 HKFRVHTFMRPAFCDHCGSLLFGLMRQGLQCEACKLNIHKRCEKNVAPHC-GVNT 296
[Close]
BLAST of SMED30016487 vs. Planmine SMEST
Match: SMESG000042308.1 (SMESG000042308.1)

HSP 1 Score: 126.331 bits (316), Expect = 3.204e-34
Identity = 61/119 (51.26%), Postives = 78/119 (65.55%), Query Frame = -1 
Query:    1 RKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGA-DTGPDTEYQNN-------HKFKPHTYTTPTFCDHCGSLLYGLFHQGIKCD 333
            R GALR++ +H++K HKF    FKQ TFC  C  FIWG   QG+QCQ+C   VHKRC++ V  +CPG  DT   +E Q N       HKF+ HT+  P FCDHCGSLL+GL  QG++C+
Sbjct:  157 RAGALRRR-IHQIKGHKFQVTLFKQFTFCSLCNSFIWGLWNQGYQCQVCTCVVHKRCYQNVIVRCPGVKDTYSSSEAQLNRFNINVPHKFRVHTFMRPAFCDHCGSLLFGLMRQGLQCE 274

HSP 2 Score: 52.7582 bits (125), Expect = 1.722e-8
Identity = 23/55 (41.82%), Postives = 31/55 (56.36%), Query Frame = -1 
Query:  124 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADT 288
            HKF    F +P FC HC   ++G  +QG QC+ C   +HKRC + V   C G +T
Sbjct:  243 HKFRVHTFMRPAFCDHCGSLLFGLMRQGLQCEACKLNIHKRCEKNVAPHC-GVNT 296
[Close]
BLAST of SMED30016487 vs. Planmine SMEST
Match: SMESG000066024.1 (SMESG000066024.1)

HSP 1 Score: 119.013 bits (297), Expect = 1.222e-31
Identity = 55/118 (46.61%), Postives = 69/118 (58.47%), Query Frame = -1 
Query:    4 RKGALRQKNVHEVKDHKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQN--------NHKFKPHTYTTPTFCDHCGSLLYGLFHQGIKC 333
            R+GA+R++ VH+ K HKF+   FKQ TFC  CK+FIWG   QG+QC +C   +HKRC   +   CPG       E            H F PH Y  PTFCDHCGSLL+G   QG++C
Sbjct:  241 RRGAMRKQRVHQTKGHKFMVTMFKQFTFCSLCKEFIWGLWYQGYQCHVCTCVIHKRCLSNMITWCPGVKESSRNEENQLARFSINVPHHFHPHNYKWPTFCDHCGSLLFGFIRQGLQC 358

HSP 2 Score: 54.299 bits (129), Expect = 4.924e-9
Identity = 33/98 (33.67%), Postives = 43/98 (43.88%), Query Frame = -1 
Query:   34 HKFLARFFKQPTFCGHCKDFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTG-------------PDTEYQNNHKFKPHTYTTPTFCDHCGSLL 288
            H F    +K PTFC HC   ++GF +QG QC++C   +HKRC + V   C G  T               D E +        T  TPTF  H   L+
Sbjct:  328 HHFHPHNYKWPTFCDHCGSLLFGFIRQGLQCKVCKLNIHKRCEKNVAKHC-GVKTKMLVERMQACGLSPADLEPRRTQPNITETPYTPTFSKHDSGLM 424
The following BLAST results are available for this feature:
BLAST of SMED30016487 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PRKCA1.979e-6582.46protein kinase C alpha [Source:HGNC Symbol;Acc:HGN... [more]
PRKCA1.296e-6282.46protein kinase C alpha [Source:HGNC Symbol;Acc:HGN... [more]
PRKCB6.509e-6176.67protein kinase C beta [Source:HGNC Symbol;Acc:HGNC... [more]
PRKCB7.432e-6176.67protein kinase C beta [Source:HGNC Symbol;Acc:HGNC... [more]
PRKCG3.361e-5574.34protein kinase C gamma [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of SMED30016487 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pkc-21.171e-5971.30Protein kinase C-like 2 [Source:UniProtKB/Swiss-P... [more]
pkc-22.047e-5671.30Protein kinase C-like 2 [Source:UniProtKB/Swiss-P... [more]
pkc-22.300e-5671.30Protein kinase C-like 2 [Source:UniProtKB/Swiss-P... [more]
pkc-22.487e-5671.30Protein kinase C-like 2 [Source:UniProtKB/Swiss-P... [more]
pkc-11.162e-4153.44Protein kinase C-like 1B [Source:UniProtKB/Swiss-... [more]
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BLAST of SMED30016487 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pkc53E2.278e-5566.94gene:FBgn0003091 transcript:FBtr0331960[more]
Pkc53E2.278e-5566.94gene:FBgn0003091 transcript:FBtr0087049[more]
Pkc53E5.362e-5162.41gene:FBgn0003091 transcript:FBtr0087048[more]
Pkc53E4.411e-4960.61gene:FBgn0003091 transcript:FBtr0112819[more]
Pkc53E4.411e-4960.61gene:FBgn0003091 transcript:FBtr0305070[more]
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BLAST of SMED30016487 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prkcba1.195e-6471.09protein kinase C, beta a [Source:ZFIN;Acc:ZDB-GENE... [more]
prkcaa8.043e-6382.46protein kinase C, alpha, a [Source:ZFIN;Acc:ZDB-GE... [more]
prkcaa2.005e-6282.46protein kinase C, alpha, a [Source:ZFIN;Acc:ZDB-GE... [more]
prkcbb6.867e-6276.92protein kinase C, beta b [Source:NCBI gene;Acc:393... [more]
prkcbb8.200e-6276.92protein kinase C, beta b [Source:NCBI gene;Acc:393... [more]
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BLAST of SMED30016487 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prkcb2.594e-6074.80protein kinase C beta [Source:NCBI gene;Acc:100038... [more]
prkcb3.536e-6075.21protein kinase C beta [Source:NCBI gene;Acc:100038... [more]
prkcb4.786e-6075.00protein kinase C beta [Source:NCBI gene;Acc:100038... [more]
prkcb6.052e-6075.00protein kinase C beta [Source:NCBI gene;Acc:100038... [more]
prkcb6.927e-6075.00protein kinase C beta [Source:NCBI gene;Acc:100038... [more]
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BLAST of SMED30016487 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Prkca8.713e-6382.46protein kinase C, alpha [Source:MGI Symbol;Acc:MGI... [more]
Prkcb5.446e-6176.67protein kinase C, beta [Source:MGI Symbol;Acc:MGI:... [more]
Prkcb6.758e-6176.67protein kinase C, beta [Source:MGI Symbol;Acc:MGI:... [more]
Prkcb6.758e-6176.67protein kinase C, beta [Source:MGI Symbol;Acc:MGI:... [more]
Prkcg7.956e-5674.34Protein kinase C gamma type [Source:UniProtKB/Swi... [more]
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BLAST of SMED30016487 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P05696|KPCA_RAT6.031e-6282.46Protein kinase C alpha type OS=Rattus norvegicus O... [more]
sp|P20444|KPCA_MOUSE6.095e-6282.46Protein kinase C alpha type OS=Mus musculus OX=100... [more]
sp|P17252|KPCA_HUMAN6.223e-6282.46Protein kinase C alpha type OS=Homo sapiens OX=960... [more]
sp|P10102|KPCA_RABIT6.289e-6282.46Protein kinase C alpha type OS=Oryctolagus cunicul... [more]
sp|Q16974|KPC1_APLCA9.408e-6279.13Calcium-dependent protein kinase C OS=Aplysia cali... [more]
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BLAST of SMED30016487 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8IRL82.570e-7178.57Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8H4R27.268e-6883.48Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267EY792.924e-6683.48Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GSY17.924e-6678.57Protein kinase C OS=Macrostomum lignano OX=282301 ... [more]
C3YC981.206e-6580.83Uncharacterized protein OS=Branchiostoma floridae ... [more]
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BLAST of SMED30016487 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prkcba1.336e-6375.20protein kinase C beta [Source:NCBI gene;Acc:103036... [more]
prkcab4.775e-6279.82protein kinase C, alpha, b [Source:ZFIN;Acc:ZDB-GE... [more]
prkcbb1.023e-6177.59protein kinase C beta type [Source:NCBI gene;Acc:1... [more]
prkcbb2.213e-6177.59protein kinase C beta type [Source:NCBI gene;Acc:1... [more]
prkcg4.373e-6074.34protein kinase C, gamma [Source:ZFIN;Acc:ZDB-GENE-... [more]
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BLAST of SMED30016487 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000002458.11.820e-3647.90pep scaffold:Pmarinus_7.0:GL479588:3:23579:-1 gene... [more]
ENSPMAT00000002460.11.936e-3647.90pep scaffold:Pmarinus_7.0:GL479588:3:23579:-1 gene... [more]
PRKCD5.092e-3349.58protein kinase C delta [Source:HGNC Symbol;Acc:HGN... [more]
ENSPMAT00000000354.11.479e-1746.15pep scaffold:Pmarinus_7.0:GL488232:2313:7448:1 gen... [more]
PRKCE1.487e-1753.97protein kinase C epsilon [Source:HGNC Symbol;Acc:H... [more]
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BLAST of SMED30016487 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
PKC15.091e-1533.33Protein serine/threonine kinase; essential for cel... [more]
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BLAST of SMED30016487 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO355852.805e-3348.21Protein kinase C [Source:UniProtKB/TrEMBL;Acc:A7S... [more]
EDO449264.315e-2763.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO449386.605e-1736.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO345911.209e-1445.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412411.995e-1126.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30016487 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prkcba2.493e-6277.31protein kinase C beta type [Source:NCBI gene;Acc:1... [more]
prkcba3.375e-6277.31protein kinase C beta type [Source:NCBI gene;Acc:1... [more]
PRKCA4.415e-6282.46protein kinase C alpha [Source:NCBI gene;Acc:10116... [more]
PRKCB6.230e-6176.07protein kinase C beta [Source:NCBI gene;Acc:105353... [more]
PRKCB1.020e-6076.07protein kinase C beta [Source:NCBI gene;Acc:105353... [more]
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BLAST of SMED30016487 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000071570.12.638e-91100.00SMESG000071570.1[more]
SMESG000039854.13.258e-6976.74SMESG000039854.1[more]
SMESG000042308.12.149e-3451.26SMESG000042308.1[more]
SMESG000042308.13.204e-3451.26SMESG000042308.1[more]
SMESG000066024.11.222e-3146.61SMESG000066024.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016487 ID=SMED30016487|Name=SMED30016487|organism=Schmidtea mediterranea sexual|type=transcript|length=465bp
ATCGCATTTGATGCCTTGATGGAACAATCCGTAGAGTAGAGATCCACAGT
GGTCACAGAAAGTTGGAGTAGTATAAGTATGTGGTTTAAATTTGTGGTTG
TTCTGATACTCTGTATCTGGACCAGTATCAGCACCTGGACATTGGAATGT
AACCAATTCATGGCATCTTTTATGGACGGCAAAAGCACATATTTGGCATT
GAAATCCCTGTTTGCCAAATCCCCAAATAAAATCTTTACAATGCCCACAG
AAAGTTGGTTGTTTGAAAAACCGAGCTAGAAATTTGTGATCTTTCACTTC
GTGTACATTTTTTTGTCGTAAAGCTCCTTTTCTTGCAAAACCTTTGAGAG
AATTATCAACAAAAGTATCTCCATATTCACTCATTAGAATTTCAATTTAT
CTCGAACTTTATTAATTTTAAAAATACAAATTTTCAAAATAGAGTTGAAA
ATTTTTACAAAAAGA
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protein sequence of SMED30016487-orf-1

>SMED30016487-orf-1 ID=SMED30016487-orf-1|Name=SMED30016487-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=128bp
MSEYGDTFVDNSLKGFARKGALRQKNVHEVKDHKFLARFFKQPTFCGHCK
DFIWGFGKQGFQCQICAFAVHKRCHELVTFQCPGADTGPDTEYQNNHKFK
PHTYTTPTFCDHCGSLLYGLFHQGIKCD
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002056medial muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR020454DAG/PE-bd
IPR002219PE/DAG-bd
Vocabulary: biological process
TermDefinition
GO:0035556intracellular signal transduction
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020454Diacylglycerol/phorbol-ester bindingPRINTSPR00008DAGPEDOMAINcoord: 71..83
score: 39.76
coord: 46..55
score: 56.83
coord: 30..44
score: 47.33
coord: 59..70
score: 50.08
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainSMARTSM00109c1_12coord: 97..127
e-value: 1.5
score: 4.2
coord: 33..82
e-value: 3.5E-15
score: 66.4
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPFAMPF00130C1_1coord: 33..84
e-value: 5.5E-18
score: 64.6
coord: 97..128
e-value: 1.6E-8
score: 34.3
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS00479ZF_DAG_PE_1coord: 33..82
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 32..82
score: 16.313
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 96..128
score: 10.668
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainCDDcd00029C1coord: 33..82
e-value: 5.42739E-14
score: 59.8132
NoneNo IPR availableGENE3DG3DSA:3.30.60.20coord: 90..128
e-value: 1.1E-14
score: 56.0
NoneNo IPR availableGENE3DG3DSA:3.30.60.20coord: 17..86
e-value: 2.7E-26
score: 93.1
NoneNo IPR availablePANTHERPTHR24356:SF158PROTEIN KINASE C BETA TYPEcoord: 11..106
NoneNo IPR availablePANTHERPTHR24356SERINE/THREONINE-PROTEIN KINASEcoord: 11..106
NoneNo IPR availableSUPERFAMILYSSF57889Cysteine-rich domaincoord: 21..83
NoneNo IPR availableSUPERFAMILYSSF57889Cysteine-rich domaincoord: 90..127