SMED30016453
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016453 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of SMED30016453 vs. TrEMBL
Match: K1QJE9 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10014990 PE=4 SV=1) HSP 1 Score: 185.652 bits (470), Expect = 5.351e-46 Identity = 168/638 (26.33%), Postives = 282/638 (44.20%), Query Frame = 2 Query: 92 MGIRGLYSY-FAFDMKAYTIFNLVQKAKVVKNMEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDLG-NVISRAYSL--TTLENLKASGVEVIKLLQGGSDEFIASEFH-RRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNY-SQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVD-HLDESVIGMIQDLENEIL 1984 MGIRGL S+ + +LV AK +EI+VDF SF + + + + +N FL I E F +F ++ I V+I D KG E +K +TW R +K ++ ++ C+ + + +L + +R L +E L + G E+I+ + G +D IA H R +++AI+SND+DF VF CR I P L DIY + E P +P + C I++ V L F + ++ LSI+ GND T Y S L + I + + A+++K N+ ++ ++ N F V FY L+ N + G + L K+S ++ L H Y E ++E+R+ IY VL + V E+ R + + I V + NL P +++ + + ++ F I SH E + + ++G + GF+ Y LRY + G N+++ +EF A+ A+VF R R ++ + ++I PT +C++I +WF+++Y A F G L L +FP E + S +V+ FY +D + V+ IQD N ++ Sbjct: 1 MGIRGLLSHCLRRREECVDEVDLVALAKSRGGIEIIVDFYSFEHMVVPKFWKGLSSLRNNQFLKILGGEYKSLETFIKRFIEIFRQLKISFVFIKDATKGCSEANSQQKLDTWMKRHHKEVENLNEVINVCRGRKEMSNLDEQMFTRPVCLEIQIVETLMSCGCEIIQSVTGEADFMIAKALHDREKAFAIWSNDSDFCVFDKCRFI-PNDLFDIYNGLQMGLPIEVP----VKPESVWCGIISSEKVRFSLGFQSPHLMVELSIIAGNDFTSQYVSTGLNRQIDIRGRIG----VETFAEWVKRYRGIENHPM----LNREMSRNVSFARAVEHSRMFYCLQS----------NPEEIVHKGYFSKLLAEKISSLKYPSHLMAMHNNFYWHRLLQEDTTRGQPCAEEALTELRALIYRIVLPRRENLVNEYGRSPWDTLRIEGVLAIDDPNL----PALHKIQEDKIFWNLNSFHYIMSHQEPVERPKWFDRYGRKNGFIVYCLRYFLLLNWGRNLQLQQQEFLALCALVFARPR------------EEHYQQIQIRPTPRCVSIGNWFLDVYRHAFLFLGKLLYLTHEFPLPEEIYSGSVWTCFYMCCKDDTYYAASRQTTQEVLSWIQDQMNAVI 599
BLAST of SMED30016453 vs. TrEMBL
Match: A0A1S3HAE6 (uncharacterized protein LOC106153553 OS=Lingula unguis OX=7574 GN=LOC106153553 PE=4 SV=1) HSP 1 Score: 168.703 bits (426), Expect = 3.322e-40 Identity = 153/613 (24.96%), Postives = 264/613 (43.07%), Query Frame = 2 Query: 92 MGIRGLYSYFAFDM-KAYTIFNLVQKAKVVKNMEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDLG---NVISRAYSLTTLENLKASGVEVIKLLQGGSDEFIASEF-HRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGI--AESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKR---RDAEGILINVVHS--PFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFY 1894 MG+RGL SY + + ++L+++A +EIL+D+ SF++ + + + N +L + E + + +GI +V+ DG KG +K ETW R K + L + CK M ++ L NV + L LK E+I+ G +D IA HR +++A+ SND+DF + ++ R I P L D+ + ++P L ++T V + F +H ++ LSI+ GND T +Y ++L L + A+ ++ + R K I+++ + ++ L NP+F V FYTL D + L+ +L++ + + ++ H Y F +E + +R+ IY VL ++V E+ R D + I ++ H+ P SN+ KPD E + +F I H E LNF ++G + GF+ ILRY + G N+ +T EF ++ A F + + + I P+ +C+TI +WF +Y A F G L L ++FP + + + AFY Sbjct: 1 MGVRGLLSYCLCNQEQCVEYYDLIEEANRKGGLEILIDYYSFQQMILTNFWKSLRAVKHNPYLRLEGGEYSTLDAYLSKLVQDLQSLGIHMVFYIDGAKGSSYFSTQQKLETWKSRHYKELRTLKQLTDVCKGVMDIQALPDDVNVRPVLLEVQVLGTLKKCNCEIIQNSCGEADFVIAEGLIHRPKAFAVLSNDSDFCIMQNSRFI-PNELFDLDNA----IQLGQASLLPQKPISLKVGVITTERVLQLFGFRSHRLLVDLSIVNGNDFTAHYMKSLYNLLDVRGAKKVENFAGWMRHYKGIENHPI----------LARELQYNPQFATAVMTSRQFYTLTAPP----------DRPTMKCPLSEYLEQAVQHGALPSNIMAMHSNFYWHRFLLEDSTPGLPCAEVALQSLRTFIYRMVLPRYENYVYEYGRTPDSDLKTIQVHAAHASLPPVSNI---KPDRTFE-------SLKLFHRIMMHQEPDFELNFFQRYGRKSGFIFCILRYFLVLNWGQNLYITPVEFLSVLATAF----------GWEHHKDQWYQNIAIRPSSRCVTIGNWFQALYRYAYNFLGQMLYLNQEFPPPHEIFAGARWTAFY 568
BLAST of SMED30016453 vs. TrEMBL
Match: A0A0L8GFX0 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22034150mg PE=4 SV=1) HSP 1 Score: 166.777 bits (421), Expect = 1.605e-39 Identity = 165/660 (25.00%), Postives = 295/660 (44.70%), Query Frame = 2 Query: 92 MGIRGLYSYFAFDMKAYTIF-NLVQKAKVVKNMEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKD-LGNVISRAY--SLTTLENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNY---SQNLLYALGIAESLDRWPHISRVAKYIKS-NHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLE----SQD-SLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFY-----ENNVNDINKRVDHLDESVIGMIQDL---ENEILECL-DGPY 2005 MG++GL+SY + K F +LV+ A+ K +EILVD+ F + + + ++N N +L + E S + + I L ++ DG +G + + TW R K + +L N++ ++D L + I R + L G +V+ G +D+ +A + +++AIFS D+DF +FKDC I P++ DI+ D M+ E G K+P L +++ V S+ +E+I L+I+ GND T +Y +Q LLY L I E R + + ++KS + + L D + + N EF V FY L L+ + GSLT + +L + + +H G Y E + + I T++S +RS IY +L + V ++ + I+ + V L P E ++ + + + IF +I + E +QD N+ ++G GF+ Y LRY + N+ +T +EF + ++F T+ + + + PT +C+TI +W +I+ A + G L L ++FP+ + S + ++ +Y EN+ +N L E + D+ + +++CL DG + Sbjct: 1 MGVQGLFSYCLKNQKQCCTFVDLVEVAREQKGIEILVDYYCFANWVYDKFMTNLLNINKNKYLLLYGGEYSSLSLYVTALIKNLQSVDIHLTFLIDGARGSCKETTKRRLNTWLSRSQKDMRKIKNMLSVFYNEISIEDLLCDDIVRPVLRDMIFTSALTECGCKVVN-CSGEADDMMAKQLLDSKAFAIFSYDSDFCLFKDCCCIPPKLF-DIHND----MELEYCGEVPKKPLHLQIGLISTERVSHMFGLSS-DELIELAIVCGNDLTSHYMRNNQTLLYKLDIRE---RKNQLENIVAWVKSYKRIENHPLLDNE-----MRNNSEFALAVQLSRSFYKLE---------LVQDYDDNNKGSLTELVIERLKTKTLHPKMLGAHHGLYWQKVLFEDLYNYWPGIETLLSNLRSCIYRILLPLNEMGVIDYGWDQSLTIMPHTVSVAKWKWL----PQFEEIKKENISHNLKIFHKIITQQEPGWKTQDWPRNYFIRYGRHNGFIFYCLRYFLLLNWQHNLFITEREFLTLICLLF------------THKEEKQYQRYPLTPTSRCITINNWMQDIFHHAYGYLGTLLFLTEEFPRPATLFSGSTWVVYYWCFCSENSPAQLNSEQRKLFEETRHKLNDIIYEKRHMIKCLVDGVF 620
BLAST of SMED30016453 vs. TrEMBL
Match: A0A210QAR5 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT01511 PE=4 SV=1) HSP 1 Score: 164.466 bits (415), Expect = 7.375e-39 Identity = 156/610 (25.57%), Postives = 265/610 (43.44%), Query Frame = 2 Query: 92 MGIRGLYSYFAFDMKAYTI-FNLVQKAKVVKNMEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVE---CDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDLG-NVISRAYSLTTLENLKASGVEVIKLLQGGSDEFIASEFHRRQ-SYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLD-RWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQ--DSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFY 1894 MG+RGL S A +L+Q A+ +EILVD+ SF + E + + ND+L I E D++ K ++ +GI+LV+ DG +G EK +TW R ++ + ++ C MP+ +L NV + L+ EV + G +D IA H+R+ +YA+FSND+DF +F+DC I PR D+ D + P +P +L ++T V L H+ ++ LSI+ GND T Y ++ G+ + L+ + I VA +I+ + V + + + N F+ V +FY L + P S G L+ L++ + + + H Y +E + ++ +R+ +Y VL V E+ R E + V + SN+ P + + + + + F I SH+E + + ++G + GF Y+LRY + G N+ +T EF A+ AM S+ P + G+ + PT +C+T+ SW+ ++Y A F G L++ +FP S V+ FY Sbjct: 1 MGVRGLLSACMRQRNACVHEVDLIQVARERGGIEILVDYYSFELFVMEQFWFSLCHHQHNDYLWILGGEYASLDVYLKKLIQH---LKALGIQLVFFVDGARGASTETTREKLDTWIERHHRELGKIRDIMSVCCGFMPITELQENVRATLQEEQFKHTLRQCDCEVYQDPAGEADCVIAQALHQREKAYAVFSNDSDFCIFRDCTFI-PRTQFDLMGDLQLGAVCDMP----IKPERLMVGVITTDRVMAILGLPRHDLLVELSIVGGNDFTGQY----IHKGGLQQKLEIKGRGIEGVADWIRDHTVPETHPV----FAQEMRHNMGFRGAVVHSRNFYNL---------GVPPLKP-SKTGHLSQILEKGIREGHFVARVMGMHNNFYWHRAVVEDLSEGQPCAEVALAPLRAHVYRIVLQRHESMVEEYGRSPWEPMRTAGVLAVEDSNI----PSIHRIQPDKIFWNLKHFHYIMSHMERRTGSESKWFERYGRKTGFSVYLLRYFLLLNWGRNLFVTENEFLALLAM-------SLGRPSAAK-----YQGICVRPTPRCVTLGSWYQDLYYHAYCFLGKLLSISHEFPLPCEAYSGAVWTVFY 568
BLAST of SMED30016453 vs. TrEMBL
Match: A0A2T7PUB9 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_04002 PE=4 SV=1) HSP 1 Score: 149.828 bits (377), Expect = 6.993e-34 Identity = 158/619 (25.53%), Postives = 265/619 (42.81%), Query Frame = 2 Query: 92 MGIRGLYSYFA-FDMKAYTIFNLVQKAKVVKNMEILVDFSSFRRDLDEIILRKIINDFD-NDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDL---GNVISRAYSLTTLENLKASGVEVIKLLQGGSDEFIASEF-HRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLD--RWPHISRVAKYIKSN-HVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHF-GRYSCVFWIEKPID---AFATITTVMSEIRSRIYPYVLHADLDHVTEFK---RRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVY--QFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFY 1894 MGIRGL S + NL++ A +E++VDF SF + L K + F N+ L I E + +GI V++ DG KG K +TW R +LE C + +L + + ++ L+ +G EVI++ +G +D IA +F R ++YA+ SND+DF +FK+ I P+ L DI D P ++PT+L C +V V + L+F E ++ LSI+ GND T + + G A+ L + I A +++ + V N +F + M N N V FY+L LI DG + + + + + Y ++F Y C +W +D A +++ +R+ +Y +L VTE+ R+D + + V+ NL P R+N + + +F I + LE + + ++G + GF+ YILRY + G + +T EF A+ A F + + + +++CP +CLTI + F +IY A F G L+L +FP + + S +++ Y Sbjct: 1 MGIRGLMSVIQKHSSECSQTVNLIEIAHEKGGIELVVDFYSFEHLVITDSLWKSLGSFKMNESLRIMGAEYATIDAYISKLITDLKSLGISFVFVLDGSKGSSYADTRHKIDTWKRRHRNDVQRKRDILEMCSGHKLMTELQEDAAIRPVLLEIQVVQALRDAGCEVIQVAEGEADLIIAKQFAEREKAYAVLSNDSDFCIFKNTCFI-PKCLFDINGDLGLGQSLALP----QKPTQLICGVVASQRVQQFLKFPAVEMLVELSIVAGNDFTGPHMPH-----GFADRLQVANYRSILSYADWVRQHVQVENNQVFKQEMM-----RNRNLANAVEHSRKFYSL-------DADLIG--EAGKDGYIFNLIVDR-----VKSGQYPANFVSIYRCFYWYRYILDDKYLCAPAEELLAPLRADLYAILLPRHKAFVTEYGQTMRQDFQQMQNYAVND---KNL----PSLNHIRENQIFRNMRLFHYIMTRLEPGQAPEKTWFDKYGRKTGFIFYILRYFLLLNWGQCLNVTEAEFLALVATAFGASK------------ESSWQELRVCPDPRCLTIAALFQDIYRHAFLFLGSVLHLKHEFPLPKEIFSGSMWTVLY 571
BLAST of SMED30016453 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1) HSP 1 Score: 1243.8 bits (3217), Expect = 0.000e+0 Identity = 601/612 (98.20%), Postives = 605/612 (98.86%), Query Frame = 2 Query: 185 MEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDLGNVISRAYSLTTLENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK 2020 MEILVDFSSFRR LDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSN VD +LECCKNKMPVK+ +VIS AYSLT LENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYS+NLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK Sbjct: 1 MEILVDFSSFRRGLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNSVDAVLECCKNKMPVKNFKDVISWAYSLTALENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSKNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK 612
BLAST of SMED30016453 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1) HSP 1 Score: 1231.47 bits (3185), Expect = 0.000e+0 Identity = 598/612 (97.71%), Postives = 603/612 (98.53%), Query Frame = 2 Query: 185 MEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDLGNVISRAYSLTTLENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK 2020 MEILVDFSSFRR LDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSN VD +LECCKNKMPVK+ +VIS AYSLT LENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYS+NLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLE +SLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK Sbjct: 1 MEILVDFSSFRRGLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNSVDAVLECCKNKMPVKNFKDVISWAYSLTALENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSKNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLE--NSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAITK 610
BLAST of SMED30016453 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1) HSP 1 Score: 1082.01 bits (2797), Expect = 0.000e+0 Identity = 523/534 (97.94%), Postives = 527/534 (98.69%), Query Frame = 2 Query: 185 MEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDLGNVISRAYSLTTLENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFM 1786 MEILVDFSSFRR LDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSN VD +LECCKNKMPVK+ +VIS AYSLT LENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYS+NLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFM Sbjct: 1 MEILVDFSSFRRGLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNSVDAVLECCKNKMPVKNFKDVISWAYSLTALENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSKNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFM 534
BLAST of SMED30016453 vs. Planmine SMEST
Match: SMESG000033476.1 (SMESG000033476.1) HSP 1 Score: 1045.8 bits (2703), Expect = 0.000e+0 Identity = 508/569 (89.28%), Postives = 529/569 (92.97%), Query Frame = 2 Query: 92 MGIRGLYSYFAFDMKAYTIFNLVQKAKVVKNMEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDLGNVISRAYSLTTLENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYS 1798 MGIRGLYSYFAFDMKAYTIFNLVQKAKVVK+MEILVDF SFRRDLD++ILRKII DFDN++LSISCVECDIF KAYLEFH+MFAEIGIKLVWIYDGI LDEV+ +KSETW RI YSNLVD LECC+NKMP KDLGNV S LT LENLKASG EVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIY D+LPFMDTENPG FFK+PTK +CPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLS MEI D LY SHFG Y+ IEKPI A T+ V+SEIRS+IYPYVL+ADL+H+TEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVT+GLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFM Y+ Sbjct: 1 MGIRGLYSYFAFDMKAYTIFNLVQKAKVVKDMEILVDFRSFRRDLDKLILRKIIKDFDNNYLSISCVECDIFGKAYLEFHKMFAEIGIKLVWIYDGINSLDEVMCYQKSETWDCRIKNYSNLVDKFLECCQNKMPFKDLGNVRSMFNVLTALENLKASGAEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYHDKLPFMDTENPGKFFKKPTKFDCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSNMEILDPLYGSHFGMYTYKLVIEKPIVASLTVGAVLSEIRSKIYPYVLNADLNHITEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTNGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMVRYN 569
BLAST of SMED30016453 vs. Planmine SMEST
Match: SMESG000033476.1 (SMESG000033476.1) HSP 1 Score: 1018.45 bits (2632), Expect = 0.000e+0 Identity = 495/556 (89.03%), Postives = 516/556 (92.81%), Query Frame = 2 Query: 131 MKAYTIFNLVQKAKVVKNMEILVDFSSFRRDLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNLVDTLLECCKNKMPVKDLGNVISRAYSLTTLENLKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYS 1798 MKAYTIFNLVQKAKVVK+MEILVDF SFRRDLD++ILRKII DFDN++LSISCVECDIF KAYLEFH+MFAEIGIKLVWIYDGI LDEV+ +KSETW RI YSNLVD LECC+NKMP KDLGNV S LT LENLKASG EVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIY D+LPFMDTENPG FFK+PTK +CPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLS MEI D LY SHFG Y+ IEKPI A T+ V+SEIRS+IYPYVL+ADL+H+TEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVT+GLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFM Y+ Sbjct: 1 MKAYTIFNLVQKAKVVKDMEILVDFRSFRRDLDKLILRKIIKDFDNNYLSISCVECDIFGKAYLEFHKMFAEIGIKLVWIYDGINSLDEVMCYQKSETWDCRIKNYSNLVDKFLECCQNKMPFKDLGNVRSMFNVLTALENLKASGAEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYHDKLPFMDTENPGKFFKKPTKFDCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSNMEILDPLYGSHFGMYTYKLVIEKPIVASLTVGAVLSEIRSKIYPYVLNADLNHITEFKRRDAEGILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTNGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMVRYN 556 The following BLAST results are available for this feature:
BLAST of SMED30016453 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30016453 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30016453 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30016453 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30016453 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30016453 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016453 ID=SMED30016453|Name=SMED30016453|organism=Schmidtea mediterranea sexual|type=transcript|length=2498bpback to top protein sequence of SMED30016453-orf-1 >SMED30016453-orf-1 ID=SMED30016453-orf-1|Name=SMED30016453-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=644bp MGIRGLYSYFAFDMKAYTIFNLVQKAKVVKNMEILVDFSSFRRDLDEIILback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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