SMED30016440

Overview
NameSMED30016440
Smed IDSMED30016440
Length (bp)2789
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016440 (SMED30016440) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016440 (SMED30016440) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016440 (SMED30016440) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016440 (SMED30016440) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016440

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30016440SMESG000079970.1 dd_Smed_v4_10005_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016440SMESG000079970.1 dd_Smed_v4_10005_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30016440 vs. TrEMBL
Match: K7JS43 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 8.677e-17
Identity = 61/219 (27.85%), Postives = 108/219 (49.32%), Query Frame = -1 
Query:  201 GENKILLSENKISSFGNLDAMLRARNNILNLNYSAYHLKNNIGTKYRLCHIDEETQAYVFNHCQAKPNARRIKHENVLISIVAYLEKIGFEIDVEKSPKYLPNP-TKLKPDMTIRSRRNKDIHVLDLKVPYDSR-EGFDKAR-GNYVKYKDLALEIGQAFNEKATVSAIVIGCLGSWDKKNNAPLAKIGLTKAEIQSLARIACAKAVTACYHIYREHVS 848
            G++   L     + F +   + RAR ++L LN +A   +  +  + R C  D ET  +V +HC     AR+ +H N+   +V    +    I V ++   +  P   L+PD+ +R   N+ + ++D+ VP+++R E FD  R     KY  LA ++  +     TV A V+G LG+WD +N   L+ + +++     + R+  +  +     IY EHVS
Sbjct:  136 GQSNHFLRSGSFTRFCDWRFIHRARLDVLPLN-AAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDS-GYTVTVEAFVVGALGAWDPRNERVLSLLSISRYYAILMRRLMVSDTIRWSRDIYVEHVS 352
[Close]
BLAST of SMED30016440 vs. TrEMBL
Match: K7JS44 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 9.003e-17
Identity = 61/219 (27.85%), Postives = 108/219 (49.32%), Query Frame = -1 
Query:  201 GENKILLSENKISSFGNLDAMLRARNNILNLNYSAYHLKNNIGTKYRLCHIDEETQAYVFNHCQAKPNARRIKHENVLISIVAYLEKIGFEIDVEKSPKYLPNP-TKLKPDMTIRSRRNKDIHVLDLKVPYDSR-EGFDKAR-GNYVKYKDLALEIGQAFNEKATVSAIVIGCLGSWDKKNNAPLAKIGLTKAEIQSLARIACAKAVTACYHIYREHVS 848
            G++   L     + F +   + RAR ++L LN +A   +  +  + R C  D ET  +V +HC     AR+ +H N+   +V    +    I V ++   +  P   L+PD+ +R   N+ + ++D+ VP+++R E FD  R     KY  LA ++  +     TV A V+G LG+WD +N   L+ + +++     + R+  +  +     IY EHVS
Sbjct:  136 GQSNHFLRSGSFTRFCDWRFIHRARLDVLPLN-AAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDS-GYTVTVEAFVVGALGAWDPRNERVLSLLSISRYYAILMRRLMVSDTIRWSRDIYVEHVS 352
[Close]
BLAST of SMED30016440 vs. TrEMBL
Match: K7JWY8 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.006e-16
Identity = 61/219 (27.85%), Postives = 108/219 (49.32%), Query Frame = -1 
Query:  201 GENKILLSENKISSFGNLDAMLRARNNILNLNYSAYHLKNNIGTKYRLCHIDEETQAYVFNHCQAKPNARRIKHENVLISIVAYLEKIGFEIDVEKSPKYLPNP-TKLKPDMTIRSRRNKDIHVLDLKVPYDSR-EGFDKAR-GNYVKYKDLALEIGQAFNEKATVSAIVIGCLGSWDKKNNAPLAKIGLTKAEIQSLARIACAKAVTACYHIYREHVS 848
            G++   L     + F +   + RAR ++L LN +A   +  +  + R C  D ET  +V +HC     AR+ +H N+   +V    +    I V ++   +  P   L+PD+ +R   N+ + ++D+ VP+++R E FD  R     KY  LA ++  +     TV A V+G LG+WD +N   L+ + +++     + R+  +  +     IY EHVS
Sbjct:  136 GQSNHFLRSGSFTRFCDWRFIHRARLDVLPLN-AAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDS-GYTVTVEAFVVGALGAWDPRNERVLSLLSISRYYAILMRRLMVSDTIRWSRDIYVEHVS 352
[Close]
BLAST of SMED30016440 vs. TrEMBL
Match: B4P1Z7 (Uncharacterized protein OS=Drosophila yakuba OX=7245 GN=Dyak\GE12380 PE=4 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 2.888e-15
Identity = 64/223 (28.70%), Postives = 106/223 (47.53%), Query Frame = -1 
Query:  174 LLSENKISSFGNLDAMLRARNNILNLNYSAYHLKNNIGTKY-RLCHIDEETQAYVFNHCQAKPNARRIKHENVLISIVAYLEKIGFEIDVEKSPKYLPNPTKLKPDMTIRSRRNKDIHVLDLKVPYD-SREGFDKARGN-YVKYKDLALEIGQAFNEKATVSAIVIGCLGSWDKKNNAPLAKIGLTKAEIQSLARIACAKAVTACYHIYREHVSYNISGFALP 833
             + +   + F +   + RAR  +L LN +   ++   G K  R+C    E+  +V  HC    NA + +H  V+  +     ++G      +      +   L+PD+  R  R++ I ++D+ VP++   E FD ARG    KY+ LA E  +A   +  + A ++G LGSWD KN   L  +G+++     + R+  A  +     IY EHVS  I  F LP
Sbjct:  141 FIRDGSFTRFADWRFIHRARLGVLPLNGA---IRWGSGDKRCRVCGYQLESVPHVLCHCMHHSNAMQQRHNAVMDRLAKAGSRLGTPRVNCRVEGVAEDMAALRPDLVWRDERSRKIVIVDVTVPFENGAEAFDNARGEKEEKYRPLA-EALRAMGYQVKLEAFIVGALGSWDPKNERVLKTLGVSRFYAGLMRRLMVADTIRWSRDIYVEHVS-GIRQFTLP 358
[Close]
BLAST of SMED30016440 vs. TrEMBL
Match: B4NX04 (Uncharacterized protein OS=Drosophila yakuba OX=7245 GN=Dyak\GE18776 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.405e-14
Identity = 63/223 (28.25%), Postives = 106/223 (47.53%), Query Frame = -1 
Query:  174 LLSENKISSFGNLDAMLRARNNILNLNYSAYHLKNNIGTKY-RLCHIDEETQAYVFNHCQAKPNARRIKHENVLISIVAYLEKIGFEIDVEKSPKYLPNPTKLKPDMTIRSRRNKDIHVLDLKVPYD-SREGFDKARGN-YVKYKDLALEIGQAFNEKATVSAIVIGCLGSWDKKNNAPLAKIGLTKAEIQSLARIACAKAVTACYHIYREHVSYNISGFALP 833
             + +   + F +   + RAR  +L +N +   ++   G K  R+C    E+  +V  HC    NA + +H  V+  +     ++G      +      +   L+PD+  R  R++ I ++D+ VP++   E FD ARG    KY+ LA E  +A   +  + A ++G LGSWD KN   L  +G+++     + R+  A  +     IY EHVS  I  F LP
Sbjct:  242 FMRDGSFTRFADWRFIHRARLGVLPVNGA---IRWGSGDKRCRVCGYQLESVPHVLCHCMHHSNAMQQRHNAVMDRLAKAGSRLGTPRVNCRVEGVAEDMAALRPDLVWRDERSRKIVIVDVTVPFENGAEAFDNARGEKEEKYRPLA-EALRAMGYQVKLEAFIVGALGSWDPKNERVLKTLGVSRFYAGLMRRLMVADTIRWSRDIYVEHVS-GIRQFTLP 459
[Close]
BLAST of SMED30016440 vs. Planmine SMEST
Match: SMESG000052713.1 (SMESG000052713.1)

HSP 1 Score: 394.815 bits (1013), Expect = 5.266e-131
Identity = 248/285 (87.02%), Postives = 255/285 (89.47%), Query Frame = -1 
Query: 1410 MSGLSNLPRIPRLSPSVIAASQRLESLEREFATLREIYQRVKSTWSSPESTKSLFQKISRLTRARDDLIIENRGQRLTIAEQSDALRKIFTENELRARLSSRSWTREIDIIPAQRLTNNPETVPSIAPTVSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNQESVRTNDPPPAYSEFEFQTTDPDILYAPTITINQAPPQVYIDLVADEIELPDVARALESLLLTPEEIAVLTGRLPSTTCPEEHSMSASGAINPNPPSSNPRQLKRRRFPVDIDSNN 2264
            MSGLSNLPRIPRLSPSVIAASQRLESLEREFATLRE YQRVKSTWSSPESTKSLFQKISRLTRARDDLIIENRGQRLTIAEQSDALRKIFTENELRARLSSRSWTREIDIIPAQRLTNNPETVPSIAPTVSATTANPTNPTRRRPNPNRRRCERRRAEANLL                P YSEFEFQTTDPDILYAPTITIN+ PP VYIDLVADEIELPDV+RALESLLLTPEEIA+LT +LPST+ PEE S SAS AI+P  PSSN RQ KRRR PVDIDS+N
Sbjct:    1 MSGLSNLPRIPRLSPSVIAASQRLESLEREFATLREKYQRVKSTWSSPESTKSLFQKISRLTRARDDLIIENRGQRLTIAEQSDALRKIFTENELRARLSSRSWTREIDIIPAQRLTNNPETVPSIAPTVSATTANPTNPTRRRPNPNRRRCERRRAEANLL----------------PIYSEFEFQTTDPDILYAPTITINRVPPPVYIDLVADEIELPDVSRALESLLLTPEEIAILTRQLPSTSRPEEPSTSASRAISPALPSSNSRQPKRRRLPVDIDSDN 269
[Close]
BLAST of SMED30016440 vs. Planmine SMEST
Match: SMESG000069750.1 (SMESG000069750.1)

HSP 1 Score: 391.734 bits (1005), Expect = 1.573e-129
Identity = 238/285 (83.51%), Postives = 250/285 (87.72%), Query Frame = -1 
Query: 1410 MSGLSNLPRIPRLSPSVIAASQRLESLEREFATLREIYQRVKSTWSSPESTKSLFQKISRLTRARDDLIIENRGQRLTIAEQSDALRKIFTENELRARLSSRSWTREIDIIPAQRLTNNPETVPSIAPTVSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNQESVRTNDPPPAYSEFEFQTTDPDILYAPTITINQAPPQVYIDLVADEIELPDVARALESLLLTPEEIAVLTGRLPSTTCPEEHSMSASGAINPNPPSSNPRQLKRRRFPVDIDSNN 2264
            M GLSNLPRIPRLSPSV A SQRLESLEREFATL E YQRVKSTWSSPE+TK LFQKI+RLTRARDDLIIENRGQRLTIAEQS ALRKIFTENELRARLSSRSW R+IDIIPA+RLTN+ E+  SI P VS TT  PTNP+RRRPN N R  ERRRAEANLLRANER Q S   ND PPAY+EFEFQTTDPDILYAPTITIN+APPQVYIDLVADEIELPDV RALE LLLTPEEIAVLTGRLPSTT PEE SMSASG I+P P +SNPRQ KRRR PVDIDS+N
Sbjct:    1 MLGLSNLPRIPRLSPSVTAVSQRLESLEREFATLWEKYQRVKSTWSSPETTKGLFQKITRLTRARDDLIIENRGQRLTIAEQSVALRKIFTENELRARLSSRSWARDIDIIPAKRLTNDSESTSSIVPPVSVTTVTPTNPSRRRPNRNSRHRERRRAEANLLRANERVQGSSPMNDLPPAYTEFEFQTTDPDILYAPTITINRAPPQVYIDLVADEIELPDVGRALERLLLTPEEIAVLTGRLPSTTGPEEPSMSASGEISPTPSNSNPRQPKRRRLPVDIDSDN 285
[Close]
BLAST of SMED30016440 vs. Planmine SMEST
Match: SMESG000061002.1 (SMESG000061002.1)

HSP 1 Score: 334.724 bits (857), Expect = 3.191e-107
Identity = 204/310 (65.81%), Postives = 233/310 (75.16%), Query Frame = -1 
Query: 1419 SEFA*QFEISRLSSVTFYQSNISIPKAMSGLSNLPRIPRLSPSVIAASQRLESLEREFATLREIYQRVKSTWSSPESTKSLFQKISRLTRARDDLIIENRGQRLTIAEQSDALRKIFTENELRARLSSRSWTREIDIIPAQRLTNNPETV-PSIAPTVSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNQESVRTNDPPPAYSEFEFQTTDPDILYAPTITINQAPPQVYIDLVADEIELPDVARALESLLLTPEEIAVLTGRLPSTTCPEEHSMSASGAINPNPPSSNPRQLKRRRFPVDID 2345
            SE   +FEI  L  V   +  +S  K M GLS+LPRIPRL PS    SQRLE LER+FATL+E YQRVKSTWSSPE+TKSLFQKISRL+RA+DDL+IEN+GQR+TIAEQS ALR+IFTENELRARL SRS  REID+IPAQRLTN+P ++ P I P  +A     T      P     R  RR AEANLLRANER Q  V T+DP PAY+EF+F T DPDILYAP+ITIN+APPQVYIDLV DE ELPDV+R LE+ LLTPEEIAVLTG+LPSTT PEE S S   A++PN     PR+ KRR  PVDID
Sbjct:   11 SEVTQRFEILLLLFVISLKLIVSSSKMMPGLSSLPRIPRLLPSATVTSQRLEILERDFATLKEKYQRVKSTWSSPEATKSLFQKISRLSRAKDDLVIENQGQRITIAEQSAALRRIFTENELRARLPSRSCAREIDVIPAQRLTNDPGSISPIILPANTA-----TKNQTGAPRRRPNRNRRRLAEANLLRANERIQGLVPTSDPSPAYTEFDFPTADPDILYAPSITINRAPPQVYIDLVTDETELPDVSRVLETQLLTPEEIAVLTGQLPSTTHPEEPSTSIQEAVHPNSSDPIPRRPKRRCLPVDID 315
[Close]
BLAST of SMED30016440 vs. Planmine SMEST
Match: SMESG000002079.1 (SMESG000002079.1)

HSP 1 Score: 333.954 bits (855), Expect = 3.860e-105
Identity = 168/196 (85.71%), Postives = 176/196 (89.80%), Query Frame = -1 
Query:  192 NNILN-LNYSAYHLKNNIGTKYRLCHIDEETQAYVFNHCQAKPNARRIKHENVLISIVAYLEKIGFEIDVEKSPKYLPNPTKLKPDMTIRSRRNKDIHVLDLKVPYDSREGFDKAR-GNYVKYKDLALEIGQAFNEKATVSAIVIGCLGSWDKKNNAPLAKIGLTKAEIQSLARIACAKAVTACYHIYREHVSYNI 773
            ++ILN   Y+   LKNNI TK RLCH+DEETQA+VFNH  AKPNARRIKHENVLISIV YLEKIGFEID+EKS KYL N TKLKPDMTIRS+RNKDIHVLDLKVPYDS EGFDKAR GNYVKYKDLALEIGQAFNEKATVSAIVIGCLG+WDKKNNAPLAKIGL KAEIQSLA IAC KAV ACYHIYREHVSYNI
Sbjct:   23 SDILNDAQYNFLRLKNNISTKCRLCHLDEETQAHVFNHWLAKPNARRIKHENVLISIVTYLEKIGFEIDLEKSLKYLSNTTKLKPDMTIRSKRNKDIHVLDLKVPYDSGEGFDKAREGNYVKYKDLALEIGQAFNEKATVSAIVIGCLGTWDKKNNAPLAKIGLNKAEIQSLAWIACPKAVIACYHIYREHVSYNI 218

HSP 2 Score: 198.364 bits (503), Expect = 3.280e-55
Identity = 120/222 (54.05%), Postives = 154/222 (69.37%), Query Frame = -1 
Query: 1638 FYQSNIS--IPKAMSGLSNLPRIPRLSPSVIAASQRLESLEREFATLREIYQRVKSTWSSPESTKSLFQKISRLTRARDDLIIENRGQRLTIAEQSDALRKIFTENELRARLSSRSWTREIDIIPAQRLTNNPETVPSIAPTVSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNQESVRTNDPPPAYSEFEFQTTDPDILYAPTITINQAPPQVY 2297
             Y+ ++S  IP   +  + LP+IPRLS S+IA  QRLE LE++ A L+  YQ++K TW SPE+T+ LFQKISRL+RARDDL++E+R QR+ IAEQSDALR+IFTE ELRAR+   SW + +DIIPAQ L +NP   P+I P+ +    +     +RRPN N RR +RRRAEA LL A ER     R NDPPPAY EFEF   D D +YAP+I  +  PP +Y
Sbjct:  209 IYREHVSYNIPHRTTNTTELPKIPRLSRSIIATRQRLEQLEKDHAVLQTKYQKLKGTWRSPEATRKLFQKISRLSRARDDLVVESRSQRILIAEQSDALRRIFTEAELRARVPGCSWAQSLDIIPAQHLFSNPIPPPTIEPSQNIRAESRPGIAQRRPNRNHRRHKRRRAEAYLLSATERMGTGSRENDPPPAYQEFEFPEFDQDNIYAPSIINHPQPPSIY 430
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BLAST of SMED30016440 vs. Planmine SMEST
Match: SMESG000050283.1 (SMESG000050283.1)

HSP 1 Score: 328.946 bits (842), Expect = 5.214e-105
Identity = 195/293 (66.55%), Postives = 218/293 (74.40%), Query Frame = -1 
Query: 1419 FYQSNISIPKAMSGLSNLPRIPRLSPSVIAASQRLESLEREFATLREIYQRVKSTWSSPESTKSLFQKISRLTRARDDLIIENRGQRLTIAEQSDALRKIFTENELRARLSSRSWTREIDIIPAQRLTNNPETVPSIAPTVSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNQESVRTNDPPPAYSEFEFQTTDPDILYAPTITINQAPPQVYIDLVADEIELPDVARALESLLLTPEEIAVLTGRLPSTTCPEEHSMSASGAINPNPPSSNPRQLKRRRFPVDID 2297
            F +  +S    M  LS+LPRIPRL PS IAASQRLE LER+FATL+E YQRVK+TWSSPE+TKSLFQ ISRL+RARDDLIIENRGQR+TIAEQS A RKIFTENELRARLSSRSW REIDIIPAQRL N+P++   I P  SA                    +   +     R   R +   RTNDPPPAYSEFEFQTTD DILYAPTITIN+APP VYIDLVADE+ELPDV+RALESLLLTPEEI VLTG+LPST  PE+ S SA G  +PN  SS+ R+ KRR  PVDID
Sbjct:   34 FLKLILSSYNVMPELSSLPRIPRLLPSAIAASQRLEVLERKFATLQEKYQRVKNTWSSPETTKSLFQNISRLSRARDDLIIENRGQRITIAEQSVASRKIFTENELRARLSSRSWAREIDIIPAQRLANDPDSTSPIVPPNSA----------------TTGNQANTSRRRPNRNRRRRERRPRTNDPPPAYSEFEFQTTDSDILYAPTITINRAPPHVYIDLVADEMELPDVSRALESLLLTPEEITVLTGQLPSTNRPEKPSASAQGVTSPNSSSSSLRRPKRRCLPVDID 310
The following BLAST results are available for this feature:
BLAST of SMED30016440 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K7JS438.677e-1727.85C2H2-type domain-containing protein OS=Nasonia vit... [more]
K7JS449.003e-1727.85C2H2-type domain-containing protein OS=Nasonia vit... [more]
K7JWY81.006e-1627.85C2H2-type domain-containing protein OS=Nasonia vit... [more]
B4P1Z72.888e-1528.70Uncharacterized protein OS=Drosophila yakuba OX=72... [more]
B4NX041.405e-1428.25Uncharacterized protein OS=Drosophila yakuba OX=72... [more]
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BLAST of SMED30016440 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016440 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052713.15.266e-13187.02SMESG000052713.1[more]
SMESG000069750.11.573e-12983.51SMESG000069750.1[more]
SMESG000061002.13.191e-10765.81SMESG000061002.1[more]
SMESG000002079.13.860e-10585.71SMESG000002079.1[more]
SMESG000050283.15.214e-10566.55SMESG000050283.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016440 ID=SMED30016440|Name=SMED30016440|organism=Schmidtea mediterranea sexual|type=transcript|length=2789bp
TTTTTTTTTCAAAATTTTATTTTAAACAACATACGGCCTTTCGGCTTAAA
ATATGCGAGCATAGCTCAGCATCTGATTCTACTGTTTCTTTACACACATT
CACAAATCTGCAACGCCGATGTAATTACCAGCATTGCTGCATAACAAACA
CATAATATTCATTTCAAGGTAAAAGGTAAGGCAAATCCACTTATGTTGTA
TGATACGTGCTCCCTGTATATGTGATAACATGCAGTCACCGCCTTTGCGC
ATGCTATCCTTGCAAGAGATTGGATCTCGGCCTTAGTCAATCCAATCTTA
GCAAGAGGTGCATTGTTTTTCTTATCCCATGATCCCAGGCATCCAATCAC
AATGGCCGATACAGTTGCTTTCTCGTTGAACGCCTGTCCGATTTCTAATG
CCAAATCTTTGTATTTAACATAGTTACCTCGCGCTTTATCAAAGCCTTCC
CTTGAATCATACGGCACTTTCAGATCAAGGACATGTATATCTTTGTTCCT
TCTTGATCTGATTGTCATGTCAGGTTTCAGCTTTGTAGGATTTGGGAGGT
ATTTTGGTGATTTCTCAACATCGATCTCAAATCCTATCTTCTCTAAGTAG
GCAACTATGCTTATTAAGACATTTTCATGCTTAATTCTTCTCGCATTAGG
CTTGGCTTGGCAGTGATTAAACACATAGGCCTGAGTTTCTTCATCAATGT
GACACAGTCTGTATTTTGTGCCAATATTGTTTTTTAGGTGATAAGCACTG
TAATTGAGATTAAGAATGTTGTTCCTAGCACGTAGCATTGCATCCAGGTT
TCCAAAGGAACTGATCTTATTTTCCGACAATAAAATCTTATTTTCTCCAA
CACCAATGGAAACCATTCAGTCAGCACTTCTATCGATCTAGGGACACCTT
TTCACGAACACCTCATGAGTTGAGATTACTGCTACGCACCCATGGCCGAA
ACCATGTTGGTACTGCGCGTAACAGCCGAAAGCTGTTCGAGCAGGTGAGC
ACTGTTTCCAGTACTACTCCTCGAAGAACCTTCGTTCCACAGTTCCTTGC
TGCCGATAGAAGGTCTTACGCTTTCTATTCCGTTTTCCCTTTCTCTAGAC
GATGTTATTTTCTGATGCTGGAGAAAAGAGTGTTAGAGTATTCCTTAAAC
ACTATTTCGATCACTCGTTGTCGACGGAGATTAACGTTGAAGTATAGGTT
TTAATTAAACATGTAATCAATTATTACTAGATACCGTTATTTTAAGTAAA
AATCAAATGAAATTAGAAAAATTTAACACTATGACAATTATATTTTTATC
CCACAGTTATTATCACCCAACGTCAAATCATTTTGTCACGTAGAGATGTC
AGTAAATAAGTTTGTTCGAGACGATGCCGGCTATTTGCTGCGCCTAGCCC
GGTGAATTAATTATTCGAGTCAATATCGACTGGGAAGCGTCGGCGTTTCA
GTTGTCTTGGGTTTGAACTTGGTGGATTTGGGTTAATTGCTCCTGAAGCT
GACATGGAATGTTCCTCGGGACAGGTGGTTGATGGCAGCCTTCCCGTCAG
AACAGCGATTTCTTCGGGGGTGAGCAGGAGACTCTCTAAGGCTCTAGCCA
CATCTGGCAGTTCTATCTCATCAGCTACCAAATCAATATACACCTGTGGC
GGTGCTTGGTTTATCGTTATTGTCGGAGCATATAGGATGTCCGGATCAGT
TGTCTGAAATTCAAATTCGGAATATGCTGGAGGTGGGTCATTTGTCCGAA
CTGATTCTTGGTTCCTTTCGTTTGCGCGCAATAAATTGGCTTCCGCACGA
CGTCTCTCGCAACGTCGGCGATTCGGATTTGGACGTCTTCTCGTGGGGTT
AGTTGGATTGGCAGTTGTCGCGCTTACGGTTGGTGCGATTGATGGCACTG
TTTCTGGGTTATTAGTGAGACGTTGGGCGGGAATGATGTCGATTTCCCGA
GTCCAACTTCGACTCGACAGACGAGCTCGGAGCTCGTTTTCCGTAAATAT
TTTCCGTAATGCATCACTTTGCTCCGCGATGGTGAGGCGTTGCCCTCTAT
TCTCGATGATTAAGTCATCTCTAGCTCTGGTCAGACGAGATATTTTCTGA
AAAAGACTCTTGGTCGATTCGGGTGAGCTCCAGGTGCTCTTCACCCGTTG
GTATATCTCTCGTAGTGTTGCAAATTCTCTCTCCAGACTTTCTAGTCGTT
GGGATGCAGCTATCACAGATGGGGACAGTCGGGGTATCCGAGGTAAGTTC
GACAATCCTGACATCGCTTTGGGGATGGATATATTTGATTGATAGAAGGT
TACTGATGATAATCGCGATATTTCAAATTGTTAGGCGAACTCCGAAAACT
GTGTTGGTCACGAATGTTTTCTGTAATAAGGAAAGATAGCACATTATAGG
GTTTATGAGTAGTATCATGGGTCTATTATCGTTATCTTTAGACGGCGTCG
TTTTGGAGTGATTAAATGGTCGGGTATTTCTGGGGTTTAACACATCTATA
CGTAATTAAAGTTTACTAGGTTTTCGTCTAGGATTCTTGTCAATTTTTGG
CGTCGTTCAAACCTTTGAACGTTAGATCTCGTCACTCTAGACCAATTTAA
TTTTCTGATGGGATTTCTGTTGACATCTCATTACATAATTTAGTCGTTCC
ACTGTTGCAACTTAACTCAATTGAATAATAGTGATTTACCATAGAATTTA
ATTACATTATGACTTGGATTTAATAGATCTGGATTTTTTACTACTAAGTT
TTATTATAGTATTCACTCGAATTAATTTATATTGTTTAC
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protein sequence of SMED30016440-orf-1

>SMED30016440-orf-1 ID=SMED30016440-orf-1|Name=SMED30016440-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=236bp
MVSIGVGENKILLSENKISSFGNLDAMLRARNNILNLNYSAYHLKNNIGT
KYRLCHIDEETQAYVFNHCQAKPNARRIKHENVLISIVAYLEKIGFEIDV
EKSPKYLPNPTKLKPDMTIRSRRNKDIHVLDLKVPYDSREGFDKARGNYV
KYKDLALEIGQAFNEKATVSAIVIGCLGSWDKKNNAPLAKIGLTKAEIQS
LARIACAKAVTACYHIYREHVSYNISGFALPFTLK*
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protein sequence of SMED30016440-orf-2

>SMED30016440-orf-2 ID=SMED30016440-orf-2|Name=SMED30016440-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=286bp
MSGLSNLPRIPRLSPSVIAASQRLESLEREFATLREIYQRVKSTWSSPES
TKSLFQKISRLTRARDDLIIENRGQRLTIAEQSDALRKIFTENELRARLS
SRSWTREIDIIPAQRLTNNPETVPSIAPTVSATTANPTNPTRRRPNPNRR
RCERRRAEANLLRANERNQESVRTNDPPPAYSEFEFQTTDPDILYAPTIT
INQAPPQVYIDLVADEIELPDVARALESLLLTPEEIAVLTGRLPSTTCPE
EHSMSASGAINPNPPSSNPRQLKRRRFPVDIDSNN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell