SMED30016425
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30016425 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 27
Alignments
SMED30016425 aligns in the following genomic locations:
Homology
BLAST of SMED30016425 vs. Planmine SMEST
Match: SMESG000064314.1 (SMESG000064314.1) HSP 1 Score: 409.068 bits (1050), Expect = 5.185e-131 Identity = 218/247 (88.26%), Postives = 227/247 (91.90%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 778
V+EG+ E+ N E +++ D S DPN+SEL E +IIEIDQ E NI+D VNVDEVSKVEETD T NLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE
Sbjct: 2761 VEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQVEKNISDPVNVDEVSKVEETDRTANLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 3007 HSP 2 Score: 261.922 bits (668), Expect = 1.077e-79 Identity = 183/340 (53.82%), Postives = 203/340 (59.71%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINEL--GGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISE-----------------------------------ESNVNE---------------LSKIDEIDRSVYPNESKLAEHYI-----------------------------------------IEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 778
VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVD GEPKQ+EQVN EEVSKVNE S D N SE + NI + G E+ N+ E+SK E D SV NE ++ + I +D+GE N+++ ESN+NE LSK+ E D S NES+ +E YI +EIDQ KNNITDPVNLNEISKVDE+ HSADLNENEPSE NINEVDE
Sbjct: 3087 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAEHYIIEIDQGENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNLNEISKVDEEDHSADLNENEPSESNINEVDE 3426 HSP 3 Score: 250.366 bits (638), Expect = 1.132e-75 Identity = 167/300 (55.67%), Postives = 188/300 (62.67%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNG-----------------------------------------SEPDDSNINEL--GGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKV----------DEKGHSADLNENEPSEYNINEVDE 778
VDEGEPKQQEQ NPE V KVN+KDNS DPN+ EL + +I EIDQGENNIAD VNVDEVSKV+E H+ + ESEP E NI EVD GEPKQ+EQ N EEV KVN+ D S DPN SEP +SNI E+ G E+ N EEVSK E DS D NE EIV+PNIK + EGET+ISEE NVNELSKIDEIDRSVY NESKLAEHYIIEIDQ +NN+ DPV ++E S +E HSAD E EP+E NINE E
Sbjct: 3005 VDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAEHYIIEIDQGENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGE 3304 HSP 4 Score: 234.572 bits (597), Expect = 3.134e-70 Identity = 151/288 (52.43%), Postives = 183/288 (63.54%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSV------------------------------------------DPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVD 775
VDEGEPKQQEQ NPEEV KVN+KDNS DPN+S L E +I+EIDQG+NNI D VN++E+SKV+E DH+ +LNE+EP ESNI EVD GEPKQ+EQVN E VSKVNE D S DP+ EP +SNINE GG+MT+EKFNL E SK E DDS DPNE E+V+P+IK + EGE NISEESNV EL+K V+ + + IE + + NITD VN ++S VDE H+ D NE+EP+E N NEVD
Sbjct: 3342 VDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNLNEISKVDEEDHSADLNENEPSESNINEVDEGEPKQQEQVNPEGVSKVNEKDNSNDPSEIEPAESNINEFGGEMTNEKFNLVEASKIFEKDDSFDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISEESNVKELTK-------VFGKQHSFDQ---IEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFNEVD 3619 HSP 5 Score: 207.223 bits (526), Expect = 1.080e-60 Identity = 129/238 (54.20%), Postives = 176/238 (73.95%), Query Frame = 2
Query: 62 QEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVD 775
Q + E LK ++ + S D + E ES+I E+D+ E + N+ EVSKV+ETDH+ + NESEP SNI E+ GE ++VN+++VSK +E+D+S DPN SEP S INE+ G++TH+K NL+EV K EN+DSVDPNE EIV+ NI+ ++EG+TN+SEESN+NE S IDE D SV PN+S+L+EHYIIEIDQ + NI+DPVN++E+SKV+E +A+LNE+EP E NI EVD
Sbjct: 2608 QTDYVKSEELKADETEYSVDQIERESAESNIDEVDE-EEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQVEKNISDPVNVDEVSKVEETDRTANLNESEPYESNIKEVD 2844 HSP 6 Score: 191.43 bits (485), Expect = 3.173e-55 Identity = 127/249 (51.00%), Postives = 167/249 (67.07%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINEL--GGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 778
+DEGE E+ N E+ K+++ D S PN+S+L E +IIEIDQ +NNI D VN++E+SKV+E H+ +LNE+EP E NI EVD GEPKQ+EQVN E VSKVNE D S DPN E NI E+ G + N++EVSK E S D E E + NI +DEGE E+ N E+ K+++ D S PN+S+L E +IIEIDQ +NNI D VN++E+SKV+E H+ +LNE+EP E NI EVDE
Sbjct: 2923 LDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDE 3171 HSP 7 Score: 176.407 bits (446), Expect = 5.281e-50 Identity = 133/288 (46.18%), Postives = 172/288 (59.72%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVS-----------------------------------------KVNENDYSRDPNGSEPDDSNINELGGKMT--HEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEV 772
VDE E KQ EQ N EEV V+ D+S +PN+ EL E +IIE+D + NI++ VN+DEVSKV+E D + NESE ESNI +VD GE KQ+EQVN EEVS KV+E D+S PN SEP +SNIN++ + T E+ N +EV D SV+P+E E+ +PN + E + NISEESNV EL K+ E + SV NE +L E IIEID+S+ NI+D VN++E+SKVDE HSAD NE+E + NINEV
Sbjct: 2191 VDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEV 2478 HSP 8 Score: 162.155 bits (409), Expect = 5.369e-45 Identity = 116/249 (46.59%), Postives = 158/249 (63.45%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINEL--GGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 778
VD GEPKQQEQ N +EV KV+ KD S D N+SEL E ++IEID+ E NI++ NV E+ V ETDH+ + N+ E + I E+D E +QVN+ V+E D+S + N SEP +S INE+ +K +L+ VSK + D S++ NE E+ + NI +DE ET E+ N E+S +D D S PNE +LAE IIE+D K NI++ VNL+E+SKVDE D NE+E +E NIN+VDE
Sbjct: 2032 VDNGEPKQQEQVNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNVKELLNVNETDHSDDQNDGELAKQIIIEIDEVEKNILDQVNV-----VDETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDE 2275 HSP 9 Score: 156.377 bits (394), Expect = 5.478e-43 Identity = 124/289 (42.91%), Postives = 163/289 (56.40%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEV-----------------------------------------SKVNENDYSRDPNGSEPDDSNINELGGKMT--HEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVD 775
VDEGE KQ EQ N EEV V+ D+S +PN+ EL E +II +D + NI++ VN+DEVSKV+E D + NESE ESNI +VD GE KQ+EQVN EEV SKV+E D+S PN SEP +SNIN++ + T ++ N E+V + E +S DPN E + PN+K +DE E NI EESNV LS +DE + S +S+LAE I +D+ + NIT NL E SKVD+ HS +LNE +E E+D
Sbjct: 1465 VDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNVKSLSNVDEKEHSTDQIKSELAEPNIRNVDEEERNITADANLKEESKVDKIDHSVNLNEKTLTEPITKEID 1753 HSP 10 Score: 150.214 bits (378), Expect = 7.366e-41 Identity = 117/248 (47.18%), Postives = 161/248 (64.92%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMT--HEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVD 775
VDE E KQQEQ N +EV+ V+ KD S +P++SEL E + IEI + + NI++ NV E+ KV ET+H+ + NE E E NI E+D E +QVN++E+SKV+E D+S DPN SE +SNINE+ K T E N +E+S+ E D+S +PN++E V+PNIK +DE E NI EES + LS ID SV NE +LAE I +++ + ++ NL E+SKVDE +S D NEN +E EVD
Sbjct: 2355 VDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVD 2602 HSP 11 Score: 147.132 bits (370), Expect = 9.664e-40 Identity = 112/247 (45.34%), Postives = 155/247 (62.75%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELG-GKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVD 775
+D+ E Q + EE K+++K S DPN+SE ES+I E+D+ E ++++EVS V++TDH+ + NES+ E NI EVDVGE EQ NLEEVS V E D S D N +EP +SNIN++ + T E+ EEVSK E D SVDPNE + V+ NIK +DE E ++ N+ ++SK+DE D SV NESK E IIE+D + + VN E+S VD+ HS D NE+E + NI ++D
Sbjct: 1752 IDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDEEETTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINL-DVSKLDETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESELANQNIIKID 1997 HSP 12 Score: 144.436 bits (363), Expect = 6.658e-39 Identity = 118/279 (42.29%), Postives = 158/279 (56.63%), Query Frame = 2
Query: 38 VDEGEPKQQEQ-FNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEES-------------------------------NVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 778
VDE EPKQQ+Q FN EE KV++KDNSDDPN++ELVE HI I +GE NI++ NV E++KV H+ + E E NI + VN ++VS V+E D++ D N SEP +SN NE+ + + + E SK E D+S DP + E+V+P IK + GE NIS ES N+ +LS++DEID SV NE L +IE+D+ + N +P NL E+SKVDE HSA+ NE+E E NI EVDE
Sbjct: 3504 VDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISEESNVKELTKVFGKQHSFDQIEGNSEERNITD----------DVNYKDVSHVDETDHADDSNESEPAESNFNEVDEEEGNSE----EESKVDEKDNSDDPYKNELVEPLIKNFNVGEKNISGESCLKDLLKVDKTEHSVNQMKCKIEERNITDDANLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDE 3768 HSP 13 Score: 144.05 bits (362), Expect = 8.966e-39 Identity = 122/288 (42.36%), Postives = 163/288 (56.60%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDP-----------------------------------------NEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 778
VDE E QEQ EEV KV +KD S DPN+S+ VE +I ++D+ E +N+D VSK++ETDH+ + NES+P ESNI EVD GE Q+EQVN EEVS V++ +S D N SE + NI ++ EK L++V+ + D SVDP NE E+ +PN+ +DE E NISE+SNV EL ++E D S N+ +LA+ IIEID+ + NI D VN+ VDE HSA+ N++EPSE INEVDE
Sbjct: 1875 VDE-EETTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINLD-VSKLDETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESELANQNIIKIDKG---EKNILDQVNVLYKTDHSVDPNKIKLAEFNINEVDNGEPKQQEQVNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNVKELLNVNETDHSDDQNDGELAKQIIIEIDEVEKNILDQVNV-----VDETDHSANSNKSEPSESKINEVDE 2152 HSP 14 Score: 138.658 bits (348), Expect = 8.474e-37 Identity = 116/289 (40.14%), Postives = 156/289 (53.98%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINEL--GGKMTHEKFNLEEV-----------------------------------------SKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVD 775
VDE + KQQ++ + + V KV+KKD S + N+SEL ES+I E+D+ E + VN +EVS V+ TDH+G NE E E NI EVDV + E+VNL+EVSKV+E D DPN SE +SNIN++ G E+ N EEV SK E D S+ PN+ E + NI +DE ET E+ N +E+ +D D+SV P+ES+L E IEI + NI++ N+ E+ KV E HS D NE E E NI E+D
Sbjct: 2150 VDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEID 2438 HSP 15 Score: 135.576 bits (340), Expect = 9.776e-36 Identity = 107/240 (44.58%), Postives = 149/240 (62.08%), Query Frame = 2
Query: 65 EQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGG--KMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 778
E FN + K+++KDNS DP +S +E+ I +ID+ E VN++EV KV+ET+ N N SE + NI +V+ E K++EQ+NLEEV+ V+E DYS D N + + E+ + ++ N EE SK E D SVD NE E+ + NI +DEGET E+ N E+S +D D S PNE +LAE II +D K NI++ VNL+E+SKVDE D NE+E +E NIN+VDE
Sbjct: 1312 ETFNLYVMTKMDQKDNSVDPYESAHIEAKINKIDE-ETIKQKQVNLEEVPKVDETEEFINPNRSELADHNIIKVE-EETKKQEQINLEEVTNVDERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDE 1549 HSP 16 Score: 134.42 bits (337), Expect = 2.295e-35 Identity = 110/248 (44.35%), Postives = 150/248 (60.48%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINE--LGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVD 775
VDE E N +E KV+K D+S + N+ L E EID+ E DHV +E SK++E ++ + NESEP ESNI EVD E KQ+++++LEEVS V++ D+S D N S+ + NI E +G + E+ NLEEVS E D SVD NE E + NI +DE ET E+ E+SK+ E D SV PNES EH I ++D+ + +NL ++SK+DE HS D NE++P E NI EVD
Sbjct: 1711 VDEEERNITADANLKEESKVDKIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDEEETT-QEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINL-DVSKLDETDHSVDTNESKPVESNIIEVD 1956 HSP 17 Score: 120.553 bits (301), Expect = 1.286e-30 Identity = 101/235 (42.98%), Postives = 150/235 (63.83%), Query Frame = 2
Query: 83 EVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGG--KMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEES-NVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDE 778
EV KV++ S+DPN++ L E E+D EN D+V +E+ K +ET+++ + E E ESNI EVD E Q E+ NL+EVSKV+E D+S DPN SEP SNINE+G K +K N+++VSK E+D SVDPN+ E P ++E E I+ + N++E+ K+DE + SV PNE ++ E I +++ K N+++ N+NE S +DE S D N++E SE+ I E+D+
Sbjct: 2575 EVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEEL-KADETEYSVDQIERESAESNIDEVDEEEITQ-EKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSE---PGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQ 2804 HSP 18 Score: 106.301 bits (264), Expect = 1.222e-25 Identity = 101/274 (36.86%), Postives = 137/274 (50.00%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVN-----------------------------------------LEEVSKVNENDYSRDPNGSEPDDSNINEL--GGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSAD 730
VDEGE KQQEQ N EEV V + +NS N++ L E E+D EN H N +E SKV+ETDH+ N+SEP ESNI +VD E KQ+EQVN ++E+ KV E ++S D N E D+ NI E+ K ++ N++E+SK E D S DPNE E NI +++ ET E N ELS++ E D S PN+ + E I +D+ + NI + L +S +D HS D
Sbjct: 2273 VDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVD 2546 HSP 19 Score: 82.4185 bits (202), Expect = 1.781e-17 Identity = 74/211 (35.07%), Postives = 104/211 (49.29%), Query Frame = 2
Query: 92 KVNKKDNSDDPNQSELVESHIIEIDQGENNIA-------------------------------DHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELG-GKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLA 628
KV++KDNSDDP ++ELVE I + GE NI+ D N+ ++S+V+E DH+ N NE N+ EVD G+ Q E NLEE+SKV+E +S +PN SE D+SNI E+ G+ +K V+PNE ++ N +D GE +E N+ E S +D+ID V P ES
Sbjct: 3630 KVDEKDNSDDPYKNELVEPLIKNFNVGEKNISGESCLKDLLKVDKTEHSVNQMKCKIEERNITDDANLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQK--------------QVNPNESTPIECNTNEVDGGEKYQTEHVNLEEESMVDKIDNFVDPKESPFT 3826 HSP 20 Score: 82.0333 bits (201), Expect = 2.452e-17 Identity = 62/162 (38.27%), Postives = 92/162 (56.79%), Query Frame = 2
Query: 74 NPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGG--KMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISE 553
N ++ +V++ D+S + N+ L ++IE+D+G+ N + N++E+SKV+E H+ N NESE ESNI EVD GE Q++QVN PN S P + N NE+ G K E NLEE S + D+ VDP E +P++ + EGE N +E
Sbjct: 3696 NLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVN---------------PNESTPIECNTNEVDGGEKYQTEHVNLEEESMVDKIDNFVDPKESPFTKPDVNKVSEGEKNKAE 3842 HSP 21 Score: 63.1586 bits (152), Expect = 4.362e-11 Identity = 50/114 (43.86%), Postives = 63/114 (55.26%), Query Frame = 2
Query: 38 VDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGS 379
VD+G+ Q E N EE+ KV++ +S +PN+SEL ES+I E+D+GE N VN NES P E N EVD GE Q E VNLEE S V++ D DP S
Sbjct: 3725 VDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVNP---------------NESTPIECNTNEVDGGEKYQTEHVNLEEESMVDKIDNFVDPKES 3823 The following BLAST results are available for this feature:
BLAST of SMED30016425 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30016425 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30016425 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30016425 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30016425 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30016425 ID=SMED30016425|Name=SMED30016425|organism=Schmidtea mediterranea sexual|type=transcript|length=779bpback to top protein sequence of SMED30016425-orf-1 >SMED30016425-orf-1 ID=SMED30016425-orf-1|Name=SMED30016425-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=259bp NENEPSESNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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