SMED30016382

Overview
NameSMED30016382
Smed IDSMED30016382
Length (bp)531
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016382 (SMED30016382) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016382 (SMED30016382) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30016382 (SMED30016382) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30016382 (SMED30016382) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016382

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30016382SMESG000029089.1 dd_Smed_v4_2873_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016382SMESG000029089.1 dd_Smed_v4_2873_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016382SMESG000029089.1 Contig29454newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016382SMESG000029089.1 Contig29454uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016382SMESG000029259.1 SMESG000029089.1 Contig31494newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30016382SMESG000029259.1 SMESG000029089.1 Contig31494uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30016382SMESG000029089.1 SMED30016382smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30016382SMESG000029089.1 SMED30016382smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
Category 3 cellSMED30016382SMESG000029089.1 dd_Smed_v6_2873_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30016382SMESG000029089.1 dd_Smed_v4_2873_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016382 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.008090:15602..16122 -2560
Homology
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BLAST of SMED30016382 vs. Planmine SMEST
Match: SMESG000029089.1 (SMESG000029089.1)

HSP 1 Score: 209.92 bits (533), Expect = 1.155e-70
Identity = 103/104 (99.04%), Postives = 103/104 (99.04%), Query Frame = 2 
Query:  110 MKQFTISSICLIVFLIVYAKASRNSDKCINILTPASKNCDVKYELNDKLCSNEINRINNFCKGVDSVECGRQYKRVETICKERSEYFWKICGSIYKMYSQECAS 421
            MKQFTI SICLIVFLIVYAKASRNSDKCINILTPASKNCDVKYELNDKLCSNEINRINNFCKGVDSVECGRQYKRVETICKERSEYFWKICGSIYKMYSQECAS
Sbjct:    1 MKQFTIWSICLIVFLIVYAKASRNSDKCINILTPASKNCDVKYELNDKLCSNEINRINNFCKGVDSVECGRQYKRVETICKERSEYFWKICGSIYKMYSQECAS 104
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BLAST of SMED30016382 vs. Planmine SMEST
Match: SMESG000028420.1 (SMESG000028420.1)

HSP 1 Score: 100.138 bits (248), Expect = 1.983e-27
Identity = 45/104 (43.27%), Postives = 65/104 (62.50%), Query Frame = 2 
Query:  110 MKQFTISSICLIVFLIVYAKASRNSDKCINILTPASKNCDVKYELNDKLCSNEINRINNFCKGVDSVECGRQYKRVETICKERSEYFWKICGSIYKMYSQECAS 421
            M + ++ ++  +V ++   K   + +KC+ ILTPA+K CD +Y   D  CS     I+  CKG DSVECG QY + +  C E+ +YFWK+CG +YK YS ECAS
Sbjct:    1 MNKISVYALFAVVVIVNSTKWVPSPEKCLKILTPAAKGCDSRYTETDLFCSVGYKHIDKSCKGHDSVECGVQYAKHKDDCTEKQDYFWKVCGPVYKTYSDECAS 104
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BLAST of SMED30016382 vs. Planmine SMEST
Match: SMESG000022139.1 (SMESG000022139.1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.925e-23
Identity = 42/104 (40.38%), Postives = 60/104 (57.69%), Query Frame = 2 
Query:  110 MKQFTISSICLIVFLIVYAKASRNSDKCINILTPASKNCDVKYELNDKLCSNEINRINNFCKGVDSVECGRQYKRVETICKERSEYFWKICGSIYKMYSQECAS 421
            MK+F+I +  + V L V       S +C N+L PASK CD  Y+  D+ C+    ++N  C G D+VECG  YK   T+CKE+ + FW  C  +Y  Y +ECA+
Sbjct:    1 MKKFSIIAFSIFVTLAVADVWIPPSARCKNVLVPASKQCDKNYKETDEFCNGGFAKLNKVCYGKDTVECGDAYKAFTTLCKEKQDNFWDECDPVYTAYRKECAT 104
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BLAST of SMED30016382 vs. Planmine SMEST
Match: SMESG000029095.1 (SMESG000029095.1)

HSP 1 Score: 88.1965 bits (217), Expect = 6.014e-23
Identity = 41/97 (42.27%), Postives = 59/97 (60.82%), Query Frame = 2 
Query:  131 SICLIVFLIVYAKASRNSDKCINILTPASKNCDVKYELNDKLCSNEINRINNFCKGVDSVECGRQYKRVETICKERSEYFWKICGSIYKMYSQECAS 421
            S+  IV+L+ Y   S   ++C   L PA+K+CD KY   D  C+    +IN  CKGVD++EC  +YK+    C+ER +YFWK CG +YK+Y   C+ 
Sbjct:    5 SLFAIVYLVAYCHCS---EQCDRELVPAAKDCDRKYAETDNFCNGGYAKINKVCKGVDTIECALKYKKFGEACRERQDYFWKNCGPVYKIYQTLCSG 98
[Close]
BLAST of SMED30016382 vs. Planmine SMEST
Match: SMESG000020501.1 (SMESG000020501.1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.228e-20
Identity = 43/97 (44.33%), Postives = 53/97 (54.64%), Query Frame = 2 
Query:  131 SICLIVFLIVYAKASRNSDKCINILTPASKNCDVKYELNDKLCSNEINRINNFCKGVDSVECGRQYKRVETICKERSEYFWKICGSIYKMYSQECAS 421
            SI  ++F+I Y   S    +C   L PA+K CD KY   D  C+     IN  CKGVD++ECG +YKR    CKER   FW  C  +YK Y   CAS
Sbjct:    5 SIFALLFVIAYCHCS---PRCDLELAPAAKRCDKKYAETDAFCNGGYAAINKVCKGVDTIECGLEYKRFAVKCKERQNNFWNDCDQVYKTYQTVCAS 98
The following BLAST results are available for this feature:
BLAST of SMED30016382 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016382 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029089.11.155e-7099.04SMESG000029089.1[more]
SMESG000028420.11.983e-2743.27SMESG000028420.1[more]
SMESG000022139.12.925e-2340.38SMESG000022139.1[more]
SMESG000029095.16.014e-2342.27SMESG000029095.1[more]
SMESG000020501.11.228e-2044.33SMESG000020501.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016382 ID=SMED30016382|Name=SMED30016382|organism=Schmidtea mediterranea sexual|type=transcript|length=531bp
CTTCCGATCTGGCTTGCGGTAGCTAATACTTTATCTGCCTTCAAGCACTA
CTACACATATATTTACTCTTATAAATTATAACATTATAAATGAGAATTCA
GATTGTACAATGAAGCAGTTTACTATTTCGTCTATTTGTTTGATAGTTTT
TTTGATAGTCTATGCCAAAGCCTCTCGAAACTCTGACAAATGTATCAACA
TTCTAACCCCAGCATCCAAAAATTGTGATGTGAAATATGAATTAAATGAT
AAATTATGTTCAAATGAAATCAACAGAATTAACAATTTCTGTAAAGGTGT
TGATTCAGTTGAGTGCGGTCGTCAATACAAGAGAGTAGAGACAATATGTA
AAGAAAGATCGGAATATTTTTGGAAAATATGTGGCTCAATTTACAAAATG
TATAGCCAAGAATGCGCCAGTGCTGCTGAAATCAAAGAAGAACATAAAGA
AATCGCTGAAGAAGCTAAAGACCAATAAACAATAAAATTGGTAATTTTAT
AGAGTACAAATGTCTTTAAATATATTGCAAT
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protein sequence of SMED30016382-orf-1

>SMED30016382-orf-1 ID=SMED30016382-orf-1|Name=SMED30016382-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=123bp
MKQFTISSICLIVFLIVYAKASRNSDKCINILTPASKNCDVKYELNDKLC
SNEINRINNFCKGVDSVECGRQYKRVETICKERSEYFWKICGSIYKMYSQ
ECASAAEIKEEHKEIAEEAKDQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
PLANA:0002142parenchyma
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 102..122
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.859
NoneNo IPR availableTMHMMTMhelixcoord: 4..21