SMED30016375

Overview
NameSMED30016375
Smed IDSMED30016375
Length (bp)501
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30016375 (SMED30016375) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30016375 (SMED30016375) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30016375

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30016375 dd_Smed_v4_18038_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30016375 dd_Smed_v4_18038_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
X1 cellSMED30016375 SmedASXL_007448SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30016375 dd_Smed_v4_18038_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30016375 dd_Smed_v4_18038_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30016375 dd_Smed_v4_18038_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30016375 dd_Smed_v4_18038_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30016375 dd_Smed_v6_18038_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
pharynx musculatureSMED30016375 dd_Smed_v4_18038_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30016375 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005291:8121..8621 -2469
v31.019732:2394..2822 -988
v31.019732:2387..2800 -694
Homology
[Close]
BLAST of SMED30016375 vs. Ensembl Xenopus
Match: ENSXETT00000043220.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:36411169:36451375:1 gene:ENSXETG00000018966.1 transcript:ENSXETT00000043220.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 65.855 bits (159), Expect = 1.347e-12
Identity = 41/91 (45.05%), Postives = 58/91 (63.74%), Query Frame = -3 
Query:   41 CSWFDSVLWKDIFPNLRISLQILL--AILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKINL 307
            C         D +PN+  + +I L   + + SC+RSFSKLKLI ++LR+S+ Q  LS+L +LS+E E   K DFD VID+F  +K+RKI L
Sbjct:  632 CQMIQDYSLADCYPNIDTAFRIFLTSPVTVASCKRSFSKLKLIKNYLRSSMEQERLSNLSILSIEHEMARKIDFDDVIDHFAAMKARKIAL 722
[Close]
BLAST of SMED30016375 vs. TrEMBL
Match: A0A087TT59 (Zinc finger MYM-type protein 1 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_17981 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 4.074e-24
Identity = 48/82 (58.54%), Postives = 60/82 (73.17%), Query Frame = -3 
Query:   41 KDIFPNLRISLQILLAI--LIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKINL 280
            +D+FPNLR+ LQILL I   I SCERSFSKLK ILS+LR S+ Q  LS L LLSVE++  +  +FD +ID F   K+RKI+L
Sbjct:  595 EDVFPNLRVGLQILLTIGTSIASCERSFSKLKHILSYLRLSMAQERLSALALLSVEQQVAQSINFDELIDKFAAAKARKISL 676

HSP 2 Score: 62.7734 bits (151), Expect = 4.074e-24
Identity = 32/78 (41.03%), Postives = 47/78 (60.26%), Query Frame = -1 
Query:  268 TKFGFLLDVKTLLSDTYVNAIRLNCKYFENFYDMDINDHEIFTEIEDRRMLISNRNEVLPETPLDVLGLILFCGKTYF 501
            + FGFLLDV  L++    ++++  C   ENFYD D++  E++TEI D RML   R +V   TP D+L  I+  G+  F
Sbjct:  521 SNFGFLLDVNGLMATGNESSLKQCCISMENFYDTDLDGLEMYTEIMDCRMLFKTRADVKLSTPEDLLRFIVQYGEDVF 598
[Close]
BLAST of SMED30016375 vs. TrEMBL
Match: T1G3L0 (Dimer_Tnp_hAT domain-containing protein OS=Helobdella robusta OX=6412 GN=20215658 PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 6.417e-23
Identity = 58/82 (70.73%), Postives = 65/82 (79.27%), Query Frame = -3 
Query:   38 DIFPNLRISLQILL--AILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKINLL 277
            D+FPNLRISLQILL  A+ I SCERSFSKLKL LS+L AS+GQ  LSDL  LSVERET E TD + +ID F  +KSRKINLL
Sbjct:    1 DVFPNLRISLQILLTTAVSIASCERSFSKLKLFLSYLSASMGQHRLSDLAFLSVERETLETTDLNNIIDQFATMKSRKINLL 82
[Close]
BLAST of SMED30016375 vs. TrEMBL
Match: A0A0J7JUM9 (52 kDa repressor of the inhibitor of the protein kinase-like protein OS=Lasius niger OX=67767 GN=RF55_25156 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.949e-22
Identity = 47/79 (59.49%), Postives = 63/79 (79.75%), Query Frame = -3 
Query:   50 KDIFPNLRISLQILLAIL--IVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRK 280
            +D+FPNLR+++QILL I   I SCERSFSKLKLIL++LR+++ Q  LSDL LL +E+ETFE  +FD +ID F  +K+RK
Sbjct:   99 EDVFPNLRVAIQILLTISVSIASCERSFSKLKLILTYLRSTMEQDRLSDLALLCIEKETFENVNFDNIIDEFATIKARK 177

HSP 2 Score: 51.2174 bits (121), Expect = 1.949e-22
Identity = 29/78 (37.18%), Postives = 45/78 (57.69%), Query Frame = -1 
Query:  268 KFGFLLDVKTLLSDTYVNA-IRLNCKYFENFYDMDINDHEIFTEIEDRRMLISNRNEVLPETPLDVLGLILFCGKTYF 498
            KFGFLLDV+ LL +  V++ +   C     FY+ D N  E++ EI D +ML+ +R E   + P ++L  I+  G+  F
Sbjct:   25 KFGFLLDVENLLKEDTVDSNLEKKCVELGEFYETDFNGAELYAEICDCKMLLRSRTESKLKIPSELLAFIISYGEDVF 102
[Close]
BLAST of SMED30016375 vs. TrEMBL
Match: T1EL67 (Dimer_Tnp_hAT domain-containing protein OS=Helobdella robusta OX=6412 GN=20197317 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 3.014e-22
Identity = 54/80 (67.50%), Postives = 66/80 (82.50%), Query Frame = -3 
Query:   44 DIFPNLRISLQILL--AILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKIN 277
            D+FPNLR++LQILL  A+ I SCERSFSKLK+ILS+LRAS+GQ  LSDL L+SVERE  E+ DFD +I+ F  VK+RKIN
Sbjct:    1 DVFPNLRVALQILLTIAVSIASCERSFSKLKIILSYLRASMGQDRLSDLALMSVEREVLEQIDFDGIINQFAAVKARKIN 80
[Close]
BLAST of SMED30016375 vs. TrEMBL
Match: T1EL66 (Dimer_Tnp_hAT domain-containing protein OS=Helobdella robusta OX=6412 GN=20197316 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 4.407e-22
Identity = 54/80 (67.50%), Postives = 66/80 (82.50%), Query Frame = -3 
Query:   44 DIFPNLRISLQILL--AILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKIN 277
            D+FPNLR++LQILL  A+ I SCERSFSKLK+ILS+LRAS+GQ  LSDL L+SVERE  E+ DFD +I+ F  VK+RKIN
Sbjct:    1 DVFPNLRVALQILLTIAVSIASCERSFSKLKIILSYLRASMGQDKLSDLALMSVEREVLEQIDFDDIINQFAAVKARKIN 80
[Close]
BLAST of SMED30016375 vs. Ensembl Cavefish
Match: ENSAMXT00000030311.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:30949094:30950197:-1 gene:ENSAMXG00000039955.1 transcript:ENSAMXT00000030311.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 4.035e-8
Identity = 32/82 (39.02%), Postives = 50/82 (60.98%), Query Frame = -3 
Query:   38 DIFPNLRISLQILLAILI--VSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKINLL 277
            D+FP L+ ++Q  L I +   SCERSFS L+ + + LR ++GQ  L  L ++S+E+E  +    D VID F  +K R+  L+
Sbjct:  283 DMFPTLKATVQAALTIPVSSCSCERSFSALRRLHTWLRRTMGQNRLHHLAVMSIEKELLDSISPDIVIDRFANLKVRRHRLV 364
[Close]
BLAST of SMED30016375 vs. Ensembl Cavefish
Match: ENSAMXT00000036272.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:11935414:11938921:1 gene:ENSAMXG00000041670.1 transcript:ENSAMXT00000036272.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 4.531e-8
Identity = 32/79 (40.51%), Postives = 52/79 (65.82%), Query Frame = -3 
Query:   50 KDIFPNLRISLQILLA--ILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRK 280
            ++++PNL I+L+I L   + + S ERSFSKLKLI ++LR+++GQ  LS L ++S+  E       D +I +F   K+R+
Sbjct:  740 QEVYPNLWIALRIALTLPVTVASAERSFSKLKLIKTYLRSTMGQERLSGLAVISINAEVAHSLSLDDLISDFAARKARR 818
[Close]
BLAST of SMED30016375 vs. Ensembl Cavefish
Match: ENSAMXT00000040801.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000046.1:3031619:3038346:1 gene:ENSAMXG00000043357.1 transcript:ENSAMXT00000040801.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 4.711e-8
Identity = 32/82 (39.02%), Postives = 50/82 (60.98%), Query Frame = -3 
Query:   38 DIFPNLRISLQILLAILI--VSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKINLL 277
            D+FP L+ ++Q  L I +   SCERSFS L+ + + LR ++GQ  L  L ++S+E+E  +    D VID F  +K R+  L+
Sbjct:  639 DMFPTLKATVQAALTIPVSSCSCERSFSALRRLHTWLRRTMGQNRLHHLAVMSIEKELLDSISPDIVIDRFANLKVRRHRLV 720
[Close]
BLAST of SMED30016375 vs. Ensembl Cavefish
Match: ENSAMXT00000035531.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000029.1:6285841:6288423:1 gene:ENSAMXG00000041958.1 transcript:ENSAMXT00000035531.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 4.511e-7
Identity = 32/79 (40.51%), Postives = 51/79 (64.56%), Query Frame = -3 
Query:   50 KDIFPNLRISLQILLAILIVSCE--RSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRK 280
            +  FPN+ I+L++ L + I +CE  RSFSKL  I + LR  + Q  L+ L LL++E +   + +FD ++D+F + KSRK
Sbjct:  779 QSTFPNVYIALRLFLTVPITNCEGERSFSKLARIKNELRIRMRQNRLNSLSLLAIESDLVRQLNFDELVDDFARKKSRK 857
[Close]
BLAST of SMED30016375 vs. Ensembl Cavefish
Match: ENSAMXT00000042715.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:11855083:11857132:-1 gene:ENSAMXG00000035113.1 transcript:ENSAMXT00000042715.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 4.682e-7
Identity = 32/79 (40.51%), Postives = 51/79 (64.56%), Query Frame = -3 
Query:   50 KDIFPNLRISLQILLAILIVSCE--RSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRK 280
            +  FPN+ I+L++ L + I +CE  RSFSKL  I + LR  + Q  L+ L LL++E +   + +FD ++D+F + KSRK
Sbjct:  577 QSTFPNVYIALRLFLTVPITNCEGERSFSKLARIKNELRIRMRQNRLNSLSLLAIESDLVRQLNFDELVDDFARKKSRK 655
[Close]
BLAST of SMED30016375 vs. Ensembl Medaka
Match: ENSORLT00000028133.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:111948955])

HSP 1 Score: 54.6842 bits (130), Expect = 5.663e-9
Identity = 32/79 (40.51%), Postives = 51/79 (64.56%), Query Frame = -3 
Query:   47 DIFPNLRISLQI--LLAILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKI 277
            +I+PN   +L+I   L + +   ERSFSKLKLI S+LR+++ Q  L++L ++S+     E+  +D VID F   K+RK+
Sbjct:  771 EIYPNFWTALRIALTLPVTVAQAERSFSKLKLIKSYLRSTMSQERLTNLAVVSINHSVGEQISYDDVIDEFASRKARKV 849
[Close]
BLAST of SMED30016375 vs. Ensembl Medaka
Match: ENSORLT00000042294.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:110016639])

HSP 1 Score: 54.6842 bits (130), Expect = 5.663e-9
Identity = 32/79 (40.51%), Postives = 51/79 (64.56%), Query Frame = -3 
Query:   47 DIFPNLRISLQI--LLAILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKI 277
            +I+PN   +L+I   L + +   ERSFSKLKLI S+LR+++ Q  L++L ++S+     E+  +D VID F   K+RK+
Sbjct:  771 EIYPNFWTALRIALTLPVTVAQAERSFSKLKLIKSYLRSTMSQERLTNLAVVSINHSVGEQISYDDVIDEFASRKARKV 849
[Close]
BLAST of SMED30016375 vs. Ensembl Medaka
Match: ENSORLT00000045044.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:111948193])

HSP 1 Score: 54.6842 bits (130), Expect = 5.663e-9
Identity = 32/79 (40.51%), Postives = 51/79 (64.56%), Query Frame = -3 
Query:   47 DIFPNLRISLQI--LLAILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKI 277
            +I+PN   +L+I   L + +   ERSFSKLKLI S+LR+++ Q  L++L ++S+     E+  +D VID F   K+RK+
Sbjct:  771 EIYPNFWTALRIALTLPVTVAQAERSFSKLKLIKSYLRSTMSQERLTNLAVVSINHSVGEQISYDDVIDEFASRKARKV 849
[Close]
BLAST of SMED30016375 vs. Planmine SMEST
Match: SMESG000070554.1 (SMESG000070554.1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.193e-28
Identity = 51/84 (60.71%), Postives = 64/84 (76.19%), Query Frame = -3 
Query:   47 VLW-KDIFPNLRISLQILL--AILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKI 289
            VL+ +D+FPNLRI+ Q+LL  A+ I SCERSFSKLKLILS+LR S+GQ  L  L L S+ER+T +  +FD VID F  VK+RKI
Sbjct:  113 VLYGEDVFPNLRIAQQMLLTIAVSIASCERSFSKLKLILSYLRESMGQERLDSLALFSIERDTLDSINFDDVIDTFAAVKARKI 196

HSP 2 Score: 56.225 bits (134), Expect = 4.193e-28
Identity = 31/77 (40.26%), Postives = 47/77 (61.04%), Query Frame = -1 
Query:  268 KFGFLLDVKTLLSDTYVNAIRLNCKYFENFYDMDINDHEIFTEIEDRRMLISNRNEVLPETPLDVLGLILFCGKTYF 498
            KFGFLL++  LLS   V+ +   C    +FYD D+N  E+F EI D ++L+  R + L  +P+D+L LI+  G+  F
Sbjct:   45 KFGFLLNINNLLSMKKVD-LEEKCLNLSSFYDSDVNGTELFNEICDTKILMKTRCDTLLSSPVDLLKLIVLYGEDVF 120
[Close]
BLAST of SMED30016375 vs. Planmine SMEST
Match: SMESG000078061.1 (SMESG000078061.1)

HSP 1 Score: 83.1889 bits (204), Expect = 5.828e-27
Identity = 50/83 (60.24%), Postives = 63/83 (75.90%), Query Frame = -3 
Query:   38 KDIFPNLRISLQILL--AILIVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKINLL 280
            +D+FPNL+I+LQ+ L  A+ I SCERSFSKLKLILS+LR S+GQ  L  L LLSVERET +  +FD VI+ F  V+ RKI L+
Sbjct:  294 EDVFPNLKIALQMFLTIAVSIASCERSFSKLKLILSYLRESMGQERLDSLALLSVERETLDSINFDDVINTFAGVEPRKIFLM 376

HSP 2 Score: 54.6842 bits (130), Expect = 5.828e-27
Identity = 31/77 (40.26%), Postives = 46/77 (59.74%), Query Frame = -1 
Query:  268 KFGFLLDVKTLLSDTYVNAIRLNCKYFENFYDMDINDHEIFTEIEDRRMLISNRNEVLPETPLDVLGLILFCGKTYF 498
            KFGFLL++  LLS   V+ +   C    +FYD D+N  E+F EI D +ML+  R + L  +P+ +L LI+  G+  F
Sbjct:  222 KFGFLLNINNLLSMKKVD-LEEKCLNLASFYDNDVNGTELFNEICDTKMLMKTRCDTLLSSPVHLLKLIVLYGEDVF 297
[Close]
BLAST of SMED30016375 vs. Planmine SMEST
Match: SMESG000000523.1 (SMESG000000523.1)

HSP 1 Score: 72.7886 bits (177), Expect = 4.468e-24
Identity = 50/83 (60.24%), Postives = 60/83 (72.29%), Query Frame = -3 
Query:   38 KDIFPNLRISLQILLAIL--IVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRKINLL 280
            +D+F  LRI+ QILL I   I SCERSFSKLKLILS+LRA +GQ  L+DL LLS++R   EK DFD V   F  VKSRK +L+
Sbjct:  101 EDVFLYLRIAPQILLTISVSIASCERSFSKLKLILSYLRALMGQARLTDLALLSIKRNEMEKRDFDEVTAKFATVKSRKRDLI 183

HSP 2 Score: 55.8398 bits (133), Expect = 4.468e-24
Identity = 26/60 (43.33%), Postives = 38/60 (63.33%), Query Frame = -1 
Query:  268 NAIRLNCKYFENFYDMDINDHEIFTEIEDRRMLISNRNEVLPETPLDVLGLILFCGKTYF 447
            N++R +C+   N YD DI   ++ TEI D +ML+  RN+ LP TPLD+L  I+  G+  F
Sbjct:   45 NSLRQHCRDLGNLYDTDIQGKKLCTEISDCQMLLRVRNDALPATPLDLLPFIISYGEDVF 104
[Close]
BLAST of SMED30016375 vs. Planmine SMEST
Match: SMESG000065712.1 (SMESG000065712.1)

HSP 1 Score: 78.9518 bits (193), Expect = 6.321e-23
Identity = 49/83 (59.04%), Postives = 62/83 (74.70%), Query Frame = -3 
Query:   50 VLW-KDIFPNLRISLQILLAIL--IVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDFDCVIDNFGKVKSRK 289
            VL+ +D FPNLRI+LQ+LL I+  I SCERSFSKLKL+L +LR S+GQ  L  L LLS+E +T +  +FD VID F  VK+RK
Sbjct:   56 VLYGEDDFPNLRIALQMLLTIVVSIASCERSFSKLKLMLFYLRESMGQERLDSLALLSIESDTLDSINFDDVIDTFAAVKARK 138

HSP 2 Score: 45.8246 bits (107), Expect = 6.321e-23
Identity = 22/55 (40.00%), Postives = 33/55 (60.00%), Query Frame = -1 
Query:  268 NCKYFENFYDMDINDHEIFTEIEDRRMLISNRNEVLPETPLDVLGLILFCGKTYF 432
             C    +FYD D+N  E+F EI D +ML+  R + L  +P+D+L LI+  G+  F
Sbjct:    9 KCLNLASFYDSDVNGTELFNEICDTKMLMKTRCDTLLSSPVDLLKLIVLYGEDDF 63
[Close]
BLAST of SMED30016375 vs. Planmine SMEST
Match: SMESG000029155.1 (SMESG000029155.1)

HSP 1 Score: 67.781 bits (164), Expect = 6.350e-22
Identity = 41/65 (63.08%), Postives = 51/65 (78.46%), Query Frame = -3 
Query:   92 KDIFPNLRISLQILLAIL--IVSCERSFSKLKLILSHLRASIGQXXXXXXXXXXVERETFEKTDF 280
            +D+FPNLRI+LQ+LL I+  I SC+RSFSKLKLILS+LR S+GQ  L  L LLSVER+T +   F
Sbjct:  327 EDVFPNLRIALQMLLTIVVSIASCQRSFSKLKLILSYLRESMGQERLDSLALLSVERDTLDSIGF 391

HSP 2 Score: 53.5286 bits (127), Expect = 6.350e-22
Identity = 30/77 (38.96%), Postives = 45/77 (58.44%), Query Frame = -1 
Query:  268 KFGFLLDVKTLLSDTYVNAIRLNCKYFENFYDMDINDHEIFTEIEDRRMLISNRNEVLPETPLDVLGLILFCGKTYF 498
            KFGFLL++  LL    V+ +   C    +FYD D+N  E+F EI D +ML+  R + L  +P+D+L L +  G+  F
Sbjct:  255 KFGFLLNINNLLRMKKVD-LEEKCLNLASFYDSDVNGIELFNEICDTKMLMKTRCDTLLSSPVDLLKLTVLYGEDVF 330
The following BLAST results are available for this feature:
BLAST of SMED30016375 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000043220.11.347e-1245.05pep primary_assembly:Xenopus_tropicalis_v9.1:7:364... [more]
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BLAST of SMED30016375 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A087TT594.074e-2458.54Zinc finger MYM-type protein 1 (Fragment) OS=Stego... [more]
T1G3L06.417e-2370.73Dimer_Tnp_hAT domain-containing protein OS=Helobde... [more]
A0A0J7JUM91.949e-2259.4952 kDa repressor of the inhibitor of the protein k... [more]
T1EL673.014e-2267.50Dimer_Tnp_hAT domain-containing protein OS=Helobde... [more]
T1EL664.407e-2267.50Dimer_Tnp_hAT domain-containing protein OS=Helobde... [more]
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BLAST of SMED30016375 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000030311.14.035e-839.02pep primary_assembly:Astyanax_mexicanus-2.0:15:309... [more]
ENSAMXT00000036272.14.531e-840.51pep primary_assembly:Astyanax_mexicanus-2.0:20:119... [more]
ENSAMXT00000040801.14.711e-839.02pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000035531.14.511e-740.51pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000042715.14.682e-740.51pep primary_assembly:Astyanax_mexicanus-2.0:16:118... [more]
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BLAST of SMED30016375 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30016375 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000028133.15.663e-940.51zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
ENSORLT00000042294.15.663e-940.51zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
ENSORLT00000045044.15.663e-940.51zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
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BLAST of SMED30016375 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000070554.14.193e-2860.71SMESG000070554.1[more]
SMESG000078061.15.828e-2760.24SMESG000078061.1[more]
SMESG000000523.14.468e-2460.24SMESG000000523.1[more]
SMESG000065712.16.321e-2359.04SMESG000065712.1[more]
SMESG000029155.16.350e-2263.08SMESG000029155.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30016375 ID=SMED30016375|Name=SMED30016375|organism=Schmidtea mediterranea sexual|type=transcript|length=501bp
CACTAAGTCACTATCGTAGACTGGTTTACATACTCTACAAAAGATTTATT
TTTCTTGATTTCACTTTACCAAAATTGTCAATTACGCAATCAAAATCTGT
TTTTTCGAATGTTTCTCTTTCAACGCTAAGAAGTACAAGATCGCTGAGAC
TAACCTGTCCAATTGATGCACGCAAATGCGACAAAATAAGTTTCAATTTG
CTGAACGACCGCTCACAGCTTACAATTAAAATTGCTAATAAAATTTGCAG
TGAAATTCGCAGATTCGGAAATATGTCTTTCCACAAAACAGAATCAAACC
AAGAACATCCAACGGAGTTTCTGGAAGAACTTCATTCCGGTTACTGATCA
GCATTCTGCGATCTTCAATTTCTGTGAAAATTTCATGGTCATTGATGTCC
ATGTCGTAGAAATTTTCAAAATATTTGCAATTTAAGCGAATTGCATTGAC
ATATGTGTCTGATAGAAGAGTCTTAACATCCAGCAGAAACCCAAATTTTG
T
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protein sequence of SMED30016375-orf-1

>SMED30016375-orf-1 ID=SMED30016375-orf-1|Name=SMED30016375-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
LSHYRRLVYILYKRFIFLDFTLPKLSITQSKSVFSNVSLSTLRSTRSLRL
TCPIDARKCDKISFNLLNDRSQLTIKIANKICSEIRRFGNMSFHKTESNQ
EHPTEFLEELHSGY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0000419prepharyngeal region
PLANA:0000421tail
PLANA:0000478pharynx musculature
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR008906HATC_C_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity